Entomology Commons^™

Egg Plastron Of The Bog Copper Butterfly Tharsalea (Epidemia) Epixanthe (Bsd. & Le C. [1835]) (Lycaenidae: Lycaeninae), David M. Wright

The Taxonomic Report of the International Lepidoptera Survey

The egg of the Bog Copper butterfly, Tharsalea (Epidemia) epixanthe, has a prominent highly-sculptured chorionic surface. Trapped within the chorion is a labyrinth of air spaces which has been proposed as a plastron for gas exchange while the egg is submerged in water. Data derived from scanning electron microscopy (SEM) confirms the plastron should function as predicted. Furthermore, the insulating air spaces should prevent water loss of the diapausing first instar larvae while overwintering.

Molecular Characterization Of Western Corn Rootworm Pyrethroid Resistance, Dariane Souza, Blair D. Siegfried, Lance Meinke, Nicholas J. Miller

Department of Entomology: Faculty Publications

Background: Western corn rootworm (WCR) pyrethroid resistance has been confirmed in the western US Corn Belt. Toxicological and biochemical studies indicated that multiple mechanisms of resistance might be involved in the resistance trait, such as enhanced metabolism and/or kdr target-site mutation(s) in the voltage-gated sodium channels. To characterize the mechanisms of WCR pyrethroid resistance at the molecular level, pairwise comparisons were made between RNA-Seq data collected from pyrethroid-resistant and -susceptible WCR populations. Gene expression levels and sodium channel sequences were evaluated.

Results: Seven transcripts exhibited significantly different expression (q ≤ 0.05) when comparing field-collected pyrethroidresistant (R-Field) and -susceptible (S-Field) WCR …

Integration Of Invasive Tree, Black Locust, Into Agro-Ecological Flower Visitor Networks Induces Competition For Pollination Services, Gerald Chikowore, Sandy Lynn Steenhuisen, Reyard Mutamiswa, Grant D. Martin, Frank Chidawanyika

All Peer-Reviewed Publications

Invasion of ecosystems by alien species is a global concern. Robinia pseudoacacia L. (Fabaceae) is one such species which impacts on native diversity and ecosystem functioning. We assessed how R. pseudoacacia affects flower visitation by arthropods in commercial apple orchards in eastern Free State, South Africa. We used paired observations to compare the abundance and diversity of flower visitors and their interactions with the two plant species. Our results showed that R. pseudoacacia flowers attract African honey bees (Apis mellifera) more than apple flowers. However, flower visitation by Diptera, particularly March flies (Bibionidae) was higher in apple flowers. Arthropods of …

Neurotoxic Zanthoxylum Chalybeum Root Constituents Invoke Mosquito Larval Growth Retardation Through Ecdysteroidogenic Cyp450s Transcriptional Perturbations, Jackson M. Muema, Joel L. Bargul, James M. Mutunga, Meshack A. Obonyo, George O. Asudi, Sospeter N. Njeru

All Peer-Reviewed Publications

Intracellular effects exerted by phytochemicals eliciting insect growth-retarding responses during vector control intervention remain largely underexplored. We studied the effects of Zanthoxylum chalybeum Engl. (Rutaceae) (ZCE) root derivatives against malaria (Anopheles gambiae) and arbovirus vector (Aedes aegypti) larvae to decipher possible molecular targets. We report dose-dependent biphasic effects on larval response, with transient exposure to ZCE and its bioactive fraction (ZCFr.5) inhibiting acetylcholinesterase (AChE) activity, inducing larval lethality and growth retardation at sublethal doses. Half-maximal lethal concentrations (LC50) for ZCE against An. gambiae and Ae. aegypti larvae after 24-h exposure were 9.00 ppm and 12.26 ppm, respectively. The active fraction …

Odor Composition Of Field Versus Laboratory Desert Locust Populations, Baldwyn Torto, Hillary Kirwa, Ruth Kihika, Saliou Niassy

All Peer-Reviewed Publications

Olfaction plays an important role in the behavioural ecology of the desert locust Schistocerca gregaria (Orthoptera:Acrididae). Different locust life stages and sexes use olfactory cues for different behaviours such as grouping, mating, oviposition, feeding, maturation and gregarization, which can be exploited for management of the desert locust. However, the full spectrum of the chemistry of volatiles released by the desert locust remains unknown. Here, we compared the volatile emissions of different life stages of a natural wild population reared in the laboratory for one generation with those of a population that has been reared in the laboratory for seven generations. …

Effect Of Moisture Stress On The Development Of False Codling Moth Thaumatotibia Leucotreta (Lepidoptera: Tortricidae) On Two Tomato Varieties, Esther Museo Patrick, Irene Koomen, Shepard Ndlela, John Mwibanda Wesonga

All Peer-Reviewed Publications

False codling moth Thaumatotibia leucotreta (Meyrick) is a pest native to sub-Saharan Africa infesting over 100 plant species including tomato. Its survival and performance are influenced by changes in precipitation and stress-related biochemical changes in the host plant. Water availability for agricultural production continues to decline due to climate change affecting drought-sensitive crops like tomatoes. Little is known on host plant–insect interactions of T. leucotreta and tomato as influenced by moisture stress. Hence, our study tested the impact of different water holding capacities (WHC) (40%, 50%, 60%, 70%, and 90%) of plant growing media on the growth of Anna F1 …

Functional Land Cover Scale For Three Insect Pests With Contrasting Dispersal Strategies In A Fragmented Coffee-Based Landscape In Central Kenya, Gladys Mosomtai, Abdelmutalab G.A. Azrag, Régis Babin, Elfatih M. Abdel-Rahman, John Odindi, Onisimo Mutanga, Henri E.Z. Tonnang, Tobias Landmann, Guillaume David

All Peer-Reviewed Publications

In the Eastern Africa highlands, the gradual transformation of natural ecosystems to smallholding coffee-based agrosystems has resulted in more fragmented landscapes. Major pests of coffee find appropriate living conditions leading to high infestation rates and the need for smallholder farmers to implement pest control measures. This study aims to understand the influence of landscapes on the ecology of three major coffee pests: the coffee berry borer (CBB), Hypothenemus hampei, and the Antestia bugs Antestiopsis thunbergii (ABT) and A. facetoides (ABF). The study was conducted on a typical smallholder coffee-based landscape in central Kenya. The pest abundance was assessed monthly for …

Mitochondrial Genomes Reveal Diverse Lineages Of Diaphorina Citri Kuwayama (Hemiptera: Sternorrhyncha: Psyllidae) In Kenya And La Réunion, Yanjing Wang, Susan Halbert, Samira Mohamed, Hélène Delatte, Bernard Reynaud, George A.C. Beattie, Paul Holford, Jinming Lu, Yijing Cen

All Peer-Reviewed Publications

The Asian citrus psyllid, Diaphorina citri, is a major threat to citrus production worldwide, as it transmits ‘Candidatus Liberibacter asiaticus’, the pathogen associated with the devastating Asian form of huanglongbing. The psyllid has spread widely from its native South Asia during the past 300 years, most recently to Africa. We report the first population genomic analyses of D. citri in Kenya and La Réunion based on next-generation sequencing of their mitochondrial genomes. We analyzed mitochondrial sequences of 264 D. citri individuals to establish genetic relationships among accessions from D. citri populations from Asia, North America and Africa. Haplotype network analysis …

Model Application Of Entomopathogenic Fungi As Alternatives To Chemical Pesticides: Prospects, Challenges, And Insights For Next-Generation Sustainable Agriculture, Bamisope Steve Bamisile, Komivi Senyo Akutse, Junaid Ali Siddiqui, Yijuan Xu

All Peer-Reviewed Publications

In the past few decades, the control of pests and diseases of cultivated plants using natural and biological measures has drawn increasing attention in the quest to reduce the level of dependence on chemical products for agricultural production. The use of living organisms, predators, parasitoids, and microorganisms, such as viruses, bacteria, and fungi, has proven to be a viable and sustainable pest management technique. Among the aforementioned, fungi, most importantly the insect-pathogenic species, have been in use for more than 150years. These include the most popular strains belonging to the genera Beauveria, Metarhizium, Isaria, Hirsutella, and Lecanicillium. Their application is …

Gambel Oak Care, Shawn Olsen, Debbie Amundsen, Shital Poudyal

All Current Publications

Gambel oak is commonly called scrub oak, but other common names are Rocky Mountain white oak and Utah white oak. The scientific name is Quercus gambelii, after William Gambel, an American naturalist. This fact sheet reviews Gambel oak's usability in the landscape, its care, growth requirements, diseases and pests, and maintenance.

Redescription Of The Allomyia Renoa (Milne) Female And Association And Description Of The Male And Larva (Trichoptera: Apataniidae), David E. Ruiter, Donald R. Givens, Hiroyuki Nishimoto

Insecta Mundi

Allomyia renoa (Milne, 1935) (Trichoptera: Apataniidae) was described from six females. The male association is verified in this paper. The original type locality information is limited: “Reno, Nev., ‘78, Morrison”. An Allomyia Banks population found at Mount Rose in Washoe County, Nevada, was compared to the A. renoa type material and found to be the conspecific. Figures, descriptions and distribution of male, female, pupal and larval A. renoa are provided.

First Record Of Orsilochides Scurrilis (Stål) (Hemiptera: Heteroptera: Scutelleridae: Pachycorinae) In The United States, With Notes On The Biology And Distribution Of U.S. Species Of Orsilochides Kirkaldy, A. W. Meeds, Joseph E. Eger Jr., S. D. Carnahan

Insecta Mundi

Orsilochides scurrilis (Stål) (Hemiptera: Heteroptera: Scutelleridae) is reported from the United States for the first time based on a specimen collected in Santa Cruz County, Arizona. A key to separate the U.S. species of Orsilochides Kirkaldy is provided. In addition, host plant records and distribution of the other two species of Orsilochides that occur in the U.S., Orsilochides guttata (Herrich-Schäffer) and Orsilochides stictica (Dallas), are analyzed through a combination of digital photo records and museum specimens.

Parasitoids (Hymenoptera: Chalcidoidea) Of The White Peach Scale, Pseudaulacaspis Pentagona (Targioni-Tozzetti) (Hemiptera: Diaspididae) On Prunus Salicina Lindl. (Rosales: Rosaceae) In South Korea, Soo-Jung Suh

Insecta Mundi

Four aphelinid and one encyrtid parasitoid species (Hymenoptera: Chalcidoidea) were collected from Pseudaulacaspis pentagona (Targioni-Tozzetti) infesting twigs and stems of Japanese plum tree (Prunus salicina Lindl.) in a survey conducted in 2020 in South Korea. These were identified as Aphytis proclia (Walker), Encarsia berlesei (Howard), Marietta carnesi (Howard), Pteroptrix orientalis (Silvestri) (Aphelinidae) and Arrhenophagus chionaspidis Aurivillius (Encyrtidae). In this paper, the list of parasitoid species of P. pentagona that occur on Japanese plum trees in South Korea is updated and a brief diagnosis and photographs of each species are provided.

The Rare Beetle Egg Parasitoid Platystasius Transversus (Thomson) (Hymenoptera: Platygastridae: Sceliotrachelinae), A New Genus And Species Record For Germany, Jessica Awad, Cristina Vasilița, Lars Krogmann

Insecta Mundi

Platystasius transversus (Thomson) (Hymenoptera: Platygastridae) is a rarely collected egg parasitoid of Leptura aurulenta Fabricius (Coleoptera: Cerambycidae). Four female specimens were found in Germany, a new country record for the genus and species. Illustrations, DNA barcodes, and an updated distribution are provided. We review its taxonomic history, biology, and ecological associations.

Wildflower Pollen Quality In Roadside Habitats, With Particular Emphasis On Hedera Helix, Aoife Mcmullin, Paul Hamilton

SURE Journal: Science Undergraduate Research Experience Journal

The quality of air is of more significance today than ever before. Human population growth and pollutants emitted through fossil fuel consumption put pressures on air quality. It is widely acknowledged that atmospheric pollutants negatively impact human health; the same is also true for the health of wildlife and plants exposed to these pollutants (Brunekreef & Holgate, 2002; EEA, 2019; Schiavoni, et al., 2017). Pollen and seed formation of flowering plants have been affected when grown in areas of poor air quality (Azzazy, 2016; Sénéchal, et al., 2015). The purpose of this research was to assess pollen of …

A Molecular Survey Of Bacterial Species In The Guts Of Black Soldier Fly Larvae (Hermetia Illucens) Reared On Two Urban Organic Waste Streams In Kenya, Marwa Shumo, Fathiya M. Khamis, Fidelis Levi Ombura, Chrysantus M. Tanga, Komi K.M. Fiaboe, Sevgan Subramanian, Sunday Ekesi, Oliver K. Schlüter, Arnold Van Huis, Christian Borgemeister

All Peer-Reviewed Publications

Globally, the expansion of livestock and fisheries production is severely constrained due to the increasing costs and ecological footprint of feed constituents. The utilization of black soldier fly (BSF) as an alternative protein ingredient to fishmeal and soybean in animal feed has been widely documented. The black soldier fly larvae (BSFL) used are known to voraciously feed and grow in contaminated organic wastes. Thus, several concerns about their safety for inclusion into animal feed remain largely unaddressed. This study evaluated both culture-dependent sequence-based and 16S rDNA amplification analysis to isolate and identify bacterial species associated with BSFL fed on chicken …

Insect Developmental Plasticity: The Role In A Changing Environment, Lindsey A. Barnes

The Cardinal Edge

Climate change has been recognized as a severe threat to biodiversity. In the rapidly growing collection of literature on the consequences of global change, researchers have recently noticed a dramatic decrease in insect populations in a wide range of habitats. Insects are extremely susceptible to climatic change, especially with regard to fluctuations in moisture and temperature. However, insects often exhibit phenotypic plasticity, where organisms will express different phenotypes when presented with a specific environmental stimulus. In developmental plasticity, environmental stimuli at the larval stage can determine adult phenotypes. This review focuses on case studies of developmental plasticity in insects, with …

Geocaching: A New Instructional Tool For Natural Resources Extension And Outreach, Jacob Smith, Dave Coyle, John Thomason, Todd Matthews, John J. Riggins

Journal of Extension

Nonnative pests and pathogens severely affect forest health and are often spread in firewood. Our objectives were to create an extension education program using “Don’t Move Firewood”-themed geocaches and travel bugs which were placed near campgrounds across Mississippi, and to assess the efficiency and effectiveness of this outreach method. Interactions with caches and travel bugs were monitored over nearly one year and summarized to provide a measure of outreach success. With a one-time per-unit cost of $45.95 for caches and $6.75 for travel bugs, these tools provide ongoing, cost-effective educational approaches well suited to augment existing or launch new outreach …

The Regulation Landscape Of Mapk Signaling Cascade For Thwarting Bacillus Thuringiensis Infection In An Insect Host, Zhaojiang Guo, Shi Kang, Qingjun Wu, Shaoli Wang, Neil Crickmore, Xuguo Zhou, Alejandra Bravo, Mario Soberón, Youjun Zhang

Entomology Faculty Publications

Host-pathogen interactions are central components of ecological networks where the MAPK signaling pathways act as central hubs of these complex interactions. We have previously shown that an insect hormone modulated MAPK signaling cascade participates as a general switch to trans-regulate differential expression of diverse midgut genes in the diamondback moth, Plutella xylostella (L.) to cope with the insecticidal action of Cry1Ac toxin, produced by the entomopathogenic bacterium Bacillus thuringiensis (Bt). The relationship between topology and functions of this four-tiered phosphorylation signaling cascade, however, is an uncharted territory. Here, we carried out a genome-wide characterization of all the MAPK orthologs …

Resistance Allele Frequencies To Cry1ab And Vip3aa20, And Performance Of Cry1a.105/Cry2ab2 Susceptible And Single/Dual-Gene Resistant Corn Earworm On Non-Bt And Low Concentrations Of Bt Diet, Shucong Lin

LSU Master's Theses

Corn earworm, Helicoverpa zea (Boddie), is an important agricultural pest and a target species of Bt corn and Bt cotton. Recently, this pest in the U.S. has developed resistance to Cry1/Cry2 corn and cotton. The objectives of this study were to 1) estimate the frequencies of resistance allele to Cry1Ab and Vip3Aa20 proteins in Louisiana populations of H. zea; 2) determine if Cry1A.105/Cry2Ab2 single/dual-gene resistance in H. zea is associated with fitness costs; and 3) investigate the effect of three low concentrations of mixed Cry1A.105/Cry2Ab2 proteins on the growth, development, and reproduction of two known Bt-susceptible strains and six …