Life Sciences Commons^™

Cross-Platform Transcriptomic Data Integration Identifies An Overactive Neuro-Immune Signature In Human Osteoarthritis Synovium, Michael D Newton, Hannah Swahn, Dana E Orange, Joseph B Lesnak, Theodore J Price, Anne-Marie Malfait, Rachel E Miller, Martin K Lotz, Tristan Maerz

Faculty, Staff and Student Publications

Objective: Chronic synovitis is associated with osteoarthritis (OA) pain, but the molecular underpinnings remain unclear. Our objective was to characterize the transcriptional phenotype of OA synovium with a focus on signaling relevant to pain.

Design: Eight publicly-available microarray and RNA-sequencing GEO datasets from human non-OA and OA subjects underwent quality control and re-analysis for differentially-expressed genes (DEGs). Cross-platform statistical integration was performed via a weighted Z-test to combine detection power across datasets. Gene set enrichment, cell type enrichment, and regulon analyses were performed. Human single-cell RNA sequencing data was used to map gene expression to cell types. Ligand-receptor interactions were …

Hubungan Karakteristik Kontak Erat Dengan Pengetahuan, Sikap, Dan Perilaku Pencegahan Tuberkulosis: Studi Potong Lintang Di Puskesmas Kenarilang, Alor, Nusa Tenggara Timur, Hanif Abadi, Ezra Maraben Lily

Jurnal Epidemiologi Kesehatan Indonesia

Indonesia ranks second globally in the number of tuberculosis (TB) cases, accounting for 10% of all global cases. In the working area of Kenarilang Public Health Center, 951 confirmed TB cases were recorded as of October 2024. Close contacts of TB patients are at high risk of infection and may become new sources of transmission if not properly managed. Knowledge, attitudes, and practices (KAP) among close contacts play a crucial role in TB prevention, aligning with the target of TB elimination by 2030. This study aims to determine the relationship between the characteristics of close contacts and their KAP levels …

Deciphering Transcriptomic Changes In Chemobrain: A Comprehensive Review, Tusar Kanta Acharya, Yuan Pan, Peter M Grace, Cobi J Heijnen, Rajasekaran Mahalingam

Faculty, Staff and Student Publications

Chemotherapy-induced cognitive impairments (CICI), colloquially known as "chemobrain," represents a profound and debilitating side effect experienced by a significant number of cancer survivors, impacting their memory, multitasking, and quality of life. This review critically evaluates the molecular mechanisms underlying CICI, with a particular focus on the insights gained from transcriptomic analyses. As cancer incidence rises globally, understanding the complex interplay between chemotherapy agents and their cognitive repercussions becomes increasingly vital. Key mechanisms implicated in CICI include blood-brain barrier disruption, neuroinflammation, and oxidative stress as a result of various chemotherapy treatments, such as doxorubicin, cisplatin, and paclitaxel. We delve into advanced …

Covered Stent Salvage For Iatrogenic Vertebral Artery Injury In Traumatic Cervical Spine Injury: A Case Report And Literature Review, Tzu-Hao Yen, Wei-Liang Chen, Ying-Lin Tseng, You-Pen Chiu, Hui-Ru Ji, Jeng-Hung Guo, Cheng-Di Chiu

BioMedicine

We report the case of a 70-year-old woman who sustained complex traumatic injuries in a motor vehicle accident, including cervical spine fractures and a high suspicion of traumatic vertebral artery injury (VAI). Initial digital subtraction angiography (DSA) revealed no evidence of vertebral artery (VA) involvement. She subsequently underwent anterior cervical discectomy and fusion (ACDF); however, an iatrogenic injury to the right VA occurred intraoperatively, necessitating emergent endovascular stenting for vascular repair. This case underscores the importance of comprehensive preoperative imaging, intraoperative vigilance, and coordinated multidisciplinary management in cervical spine trauma with potential vascular involvement.

Endoscopic Management Of Ureteric Stenosis And Calculi In Bilateral Incomplete Renal Duplication: A Case Study, Hafeez Sohaib Ahmad Warraich, Zirwa Younis, Jawairia Warraich, Khizra Warraich

BioMedicine

Renal duplication anomalies are one of the most frequent congenital urological conditions in the general population all over the world. Bilateral incomplete duplication, however, is exceedingly rare. We present a case of a 29-year-old female with bilateral incomplete duplex kidneys complicated by mid-ureteric stenosis on the right side and calculi in the left ureter and upper moiety of the left duplex kidney. Diagnostic imaging, including ultrasound, computed tomography (CT), and intravenous urography (IVU) confirmed these findings. The patient underwent successful bilateral ureteroscopy (URS) and left-sided retrograde intra-renal surgery (RIRS) for management of the above mentioned complications related to the duplex …

Effect Of Garcinol Against Arsenic-Induced Neurobehavioral Alterations And Liver And Kidney Dysfunction In Albino Mice, Abdulmohsen I. Algefare

BioMedicine

The extended exposure to inorganic arsenic is commonly linked to brain damage through oxidative and apoptotic processes. The compound known as Garcinol (GCL) has garnered significant interest due to its potential advantages for human well-being. Nevertheless, the ability of GCL to protect against toxicity generated by arsenic in the brain has not yet been investigated. Hence, our study aimed to examine the neuroprotective effectiveness of GCL in mitigating the detrimental impacts of sodium arsenite (SA) on behavioral patterns, the molecular mechanisms, apoptotic markers, and oxidative stress parameters in the brain, liver, and kidney of mice. The mice were categorized into …

Investigating The Association Between Hyperthyroidism And The Risk Of Herpes Zoster In A Cohort Study In Taiwan, Shih-Wei Lai, Yu-Hung Kuo, Kuan-Fu Liao

BioMedicine

Background: The aim of this cohort study was to investigate the association between hyperthyroidism and the likelihood of developing herpes zoster in Taiwan.

Methods: Using the National Health Insurance Research Database (NHIRD) in Taiwan, we selected individuals aged 20-84 who were newly diagnosed with hyperthyroidism between 2013 and 2020 as the hyperthyroidism group. These individuals were then matched with a control group without hyperthyroidism in a 1:1 propensity score matching for sex, age, and baseline comorbidities. The occurrence of herpes zoster was tracked in both groups until the end of the study period or until a diagnosis of herpes zoster …

An Investigation Into The Potent Anticancer, Antimicrobial, And Anti-Inflammatory Activities Of A Punica Granatum Nanoemulgel, Ahmad M Eid, Murad Abualhasan, Yara Khaliliya, Zeina Sinan, Aya Khaliliya

BioMedicine

Purpose: The objective of this study is to formulate a nanoemulgel using Punica granatum (P. granatum) seed oil and study the antibacterial, anticancer, and anti-inflammatory properties. Methods: The process to formulate a nanoemulsion from P. granatum seed oil involved using the self-nanoemulsifying technique, with Span 80 and Tween 80 serving as the emulsifying agents. Carbopol hydrogel was combined with the nanoemulsion to produce the nanoemulgel. The particle size, polydispersity index (PDI), rheological behavior, antibacterial, cytotoxic, and anti-inflammatory properties were subsequently examined.

Results: The nanoemulsion formulation with a PDI of 0.229 and a particle size of 189.44 nm was …

Metabolomics In Cancer Detection: A Review Of Techniques, Biomarkers, And Clinical Utility, Kavitha Liegelin Bernhardt, Janita R. T. Pinto

BioMedicine

Cancer poses a significant burden on global public health, contributing to high mortality rates worldwide. Ongoing diagnostic strategies have predominantly relied on imaging techniques, histopathological examination and molecular analyses which have limitations in sensitivity, and specificity. Early cancer detection is a pivotal determinant of successful treatment and patient survival rates. Metabolomic applications involve the comprehensive analysis of metabolites to understand the metabolic profile of an organism, tissue, or cell under different conditions such as lack of oxygen in tumors. The aim of this review is to provide an extensive approach of metabolomic applications in early cancer detection and to provide …

Difference In Knee Joint Structure And Lower Extremity Functioning Among People With Varying Severity Of Knee Osteoarthritis: A Cross-Sectional Study, Shubham Tawade, Rajani Mullerpatan Dr., Bela Agarwal Dr.

International Journal of Exercise Science

International Journal of Exercise Science 18(5): 910-921, 2025. Osteoarthritis (OA) is a highly prevalent musculoskeletal condition. It is reported that knee OA progressively affects lower-extremity functioning. Evidence is lacking on when there is a substantial decline in function and whether this trend differs between males and females and disease severity. This study aimed to examine influence of gender and increasing grade of knee OA on lower-extremity function. Following Institutional Ethical Committee approval and signed-informed consent, 115 participants (57-males, 58-females), aged 40-65 years, with diagnosed knee OA (grade 1-4) were recruited. Lower-extremity function was studied using reliable and valid outcome …

Amplifying Voices In The Midst Of A Substance Use Crisis: Storyvisual Approach, John E. Stewart, Victoria Wagner-Greene

The RAISE Center Journal

The United States is experiencing an unprecedented substance use crisis. Strategies are needed to engage, inform and empower young adults and others affected either directly or indirectly by substance use. Based on visual information processing and storytelling theories, the StoryVisual approach is a visual communication method that combines powerful web-sourced graphics with minimal text to relate valuable information, experiences, perspectives, and opportunities in story form. To date, more than 240 students in substance use education classes at a public university in Texas have created and shared StoryVisual products, addressing such drug-related topics as vaping, fentanyl, heroin, LSD, marijuana, and substance …

Animal Science News (August 29, 2025), Department Of Animal Science

Animal Science Newsletters

No abstract provided.

Reconstruction Of Water Storage Variability In The Aral Sea Region, Nikita Murzintcev, Sahibjamal Nietullaeva, Timur Berdimbetov, Buddhi Pushpawela, Asiya Tureniyazova, Sherly Shelton, Bakbergen Aytmuratov, Khusen Gafforov, Kanat Parakhatov, Alimjan Erdashov, Abdul-Aziz Makhamatdinov, Timur Allamuratov

Plants, Soils and Climate Student Research

The Gravity Recovery and Climate Experiment (GRACE) mission, operational from 2002 to 2017, provided critical measurements of Earth's gravity field anomalies which have been extensively used to study groundwater and terrestrial water storage (TWS) dynamics. In this research, we utilize GRACE data to identify, model, and analyze potential climate parameters contributing to the reconstruction of TWS variability in the Aral Sea Basin region (ASB). We assess the impact of climate change and anthropogenic nature management on TWS change using a quantitative method. Our analysis reveals a significant decline in the TWS at a rate of 0.44 cm year^-1 during …

Impact Of Temperature On Larval Mortality And Performance Of The Acorn Barnacle Chthamalus Fissus In San Diego, Ca, Alberto Rivera

Theses

Rising ocean temperatures and increasingly frequent marine heatwaves threaten the physiological performance and survival of marine larvae. This study quantifies the thermal tolerance, respiration, and swimming behavior of Chthamalus fissus cyprid larvae to establish a baseline for understanding their vulnerability under future climate scenarios. Larvae were collected from the Scripps Pier in La Jolla, CA and exposed to a range of temperatures (12–50°C, dependent on the experiment) to assess oxygen consumption, mortality, and swimming speeds. Oxygen consumption rates increased significantly with temperature, peaking at 30°C, with cyprids exposed to temperatures above 26°C showing significantly higher respiration rates than those below …

Low Economic Level And The Risk Of Overweight Among Indonesian Junior And Senior High School Students, Ariyanto Nugroho, Purwo Setiyo Nugroho, Amarin Yudhana, Sri Sunarti

Kesmas

Overweight and obesity among Indonesian adolescents have emerged as a pressing public health issue, reflecting global trends. This study examines the relationship between economic status and overweight prevalence among junior and senior high school students in Indonesia, using secondary data from the Global School-based Health Survey (GSHS). This study analyzed data from 9,977 students aged 11–18 years through a cross-sectional design and binary logistic regression, adjusting for dietary habits, physical activity, and sedentary behavior. Overall, 14.7% of students were overweight; the prevalence was notably higher among low-income students (27.4%) compared to high-income groups (14.2%). Students from lower economic backgrounds were …

Adults With Learning Disabilities Navigating The Transition From School To Work, Shatrece L. Jenkins

Doctoral Dissertations and Projects

The purpose of this qualitative case study was to explore the experiences of adults with learning disabilities when transitioning from academics to the workplace, with an emphasis on emotional intelligence. The two research questions asked how adults with learning disabilities described their lived experiences and experiences of emotions and emotional regulation in their transition from academic environments to the professional workplace. The theory guiding this study was the emotional intelligence theory developed by Salovey and Mayer, which was used as a determinant in building relationships and upholding social interactions. The sample consisted of 13 adults with learning disabilities working at …

Ras-Membrane Interaction And Oligomerization: There Is More Than Meets The Eye, Abraham C Sianoya, Vijay K Bhardwaj, Alemayehu A Gorfe

Faculty, Staff and Student Publications

Membrane association is fundamental to Rat sarcoma (RAS) function, driving both its physiologic signaling and oncogenic transformation. This review consolidates recent advances in the study of RAS-membrane interactions, emphasizing the molecular mechanisms underlying its membrane engagement and oligomerization. We first discuss the roles of RAS lipid modification and conformational diversity of its intrinsically disordered C-terminus in these processes, and we then examine the debate surrounding RAS dimerization and its potential role in the formation of higher-order oligomers. By integrating emerging insights into these issues, we offer our own perspectives on the driving forces of RAS oligomerization and propose potential new …

Epigenetic Modulation Of Bard1 To Enhance Anti-Vegf Therapy, Emine Bayraktar, Cristian Rodriguez-Aguayo, Elaine Stur, Sudhir Kumar, Lingegowda S Mangala, Nicholas B Jennings, Nazende Nur Bayram, Sara Corvigno, Amma Asare, Cristina Ivan, Mark S Kim, Thanh Chung Vu, Pahul Hanjra, Sangbae Kim, Adrian Lankenau Ahumada, Weichem Wu, Sanghoon Lee, Anna Szymanowska, Hulya Oztatlici, Marcos R Estecio, Ju-Seog Lee, Abhinav K Jain, Nidhi Sahni, John P Hagan, Stephen Baylin, Jinsong Liu, Gabriel Lopez-Berestein, Sunila Pradeep, Anil K Sood

Faculty, Staff and Student Publications

Despite the clinical use of anti-vascular endothelial growth factor (VEGF) antibodies (AVAs) in cancer therapy, resistance frequently develops, leading to disease progression. To address this, we identify a previously unknown role for breast cancer type 1 susceptibility protein (BRCA1)-associated RING domain 1 (BARD1) in modulating AVA sensitivity. Epigenetic modulation-via global and targeted DNA methylation-reveals BARD1 as a key regulator of angiogenesis. Sequential treatment with azacytidine overcomes AVA resistance in vivo. To enable precise epigenetic reactivation, we develop a liposomal CRISPR-deactivated Cas9 (dCas9)-TET1 system guided by BARD1-targeting single-guide RNAs (sgRNAs). This platform achieves CpG-specific demethylation of the BARD1 promoter, restores expression, …

Risk Of Major Depression In Partners Of People With Alzheimer's Disease: A National Cohort Study, Casey Crump, Jingkai Wei, Barbara G Vickrey, Alexis C Edwards, Paul E Schulz, Weiva Sieh, Jan Sundquist, Kristina Sundquist

Faculty, Staff and Student Publications

Background: Alzheimer's disease (AD) may cause significant psychosocial distress not only in the patient but also their partner. However, long-term risks of major depression in partners of AD patients are largely unknown.

Methods: A national cohort study was conducted of all 145 289 partners of people diagnosed with all-cause dementia, including 57 113 partners of people diagnosed with AD, in Sweden during 1998-2017, and 1 300 561 population-based controls. Cox regression was used to compute hazard ratios (HRs) for subsequent risk of major depression identified from nationwide outpatient and inpatient diagnoses through 2018, adjusting for sociodemographic factors and prior mental …

Biddle Physical Ability Test (Bpat) Performance By Attempt In Southern California Structural Firefighter Candidates, Robert G. Lockie, Joseph Dulla, Jennifer Meloni, Kristina A. Ross, Robin (Rob) Marc Orr, J. Jay Dawes, Tomas J. Ruvalcaba

International Journal of Exercise Science

International Journal of Exercise Science 18(8): 895-909, 2025. The Biddle Physical Ability Test (BPAT) is a job task simulation that must be completed in ≤9:34 min:s by structural firefighter candidates to be accepted to a fire training academy. This study investigated the influence of prior attempts on BPAT time. Retrospective analysis was conducted on 1435 male and 72 female candidates. All participants received equivalent instruction on how to complete the BPAT which incorporated: dry hose drag; charged hose drag; halyard raise; roof walk; attic crawl; roof ventilation; victim removal; ladder removal and carry; stair climb with hose bundle; crawling search; …

Nadph Oxidase Isoforms (Nox-2 And Nox-4) In Myocardial Ischemia/Reperfusion Injury: Platelet Rich Plasma (Prp) As A Therapeutic Agent, Barbara Y. Hargrave, Frank Lattanzio

Cardiovascular Research Symposium

Introduction: Blood flow restoration to ischemic cardiac tissue is a critical and necessary intervention that has an associated downside: reperfusion injury. Reperfusion injury is a complicated process mediated in part by a pathological increase in reactive oxygen species (ROS). Although extremely elevated levels of ROS can cause cell damage, a minimum level of ROS is necessary for activation of Hydroxylated-HIF-1a (HIF-1a) and inhibition of Peroxisome Proliferator-Activated Receptor-alpha (PPARa) during myocardial ischemic reperfusion injury (MIRI). MIRI clinical manifestations include arrhythmias, myocardial stunning, decreased ventricular contractility and no-reflow. Platelet rich plasma (PRP) has improved left ventricular (LV) function after MIRI in …

Structural And Functional Effects Of Combination Complement And Hypothermia Therapies In A Rat Model Of Hie, Angie Saadat

Cardiovascular Research Symposium

Structural and Functional Effects of Combination Complement and Hypothermia Therapies in a Rat Model of HIE

Angela Saadat^a,b,c, Haree Pallera^b,d, Frank Lattanzio^e, Richard A. Britten^f,g, Asna Sulaiman^d, Stephanie Newman^d, Tushar Shah^a,b,c,d

^aPediatrics, Macon & Joan Brock Virginia Health Sciences at Old Dominion University, Norfolk, VA, USA. ^bNeonatal Brain Institute, ^cChildren’s Specialty Group, Norfolk, VA, USA. ^dChildren’s Hospital of the King’s Daughters, Norfolk, VA, ^eDepartment of Biomedical and Translational Sciences, Macon & Joan Brock Virginia Health Sciences at Old Dominion University, Norfolk, VA, …

Abundance And Demography Of The Wood Turtle (Glyptemys Insculpta) Across Two River Systems In Michigan’S Lower Peninsula, Marianne Kelso

Masters Theses

Turtles worldwide are declining from habitat loss and fragmentation, predation, and illegal collection, and their slow life histories and sensitivity to environmental change make reliable abundance and demographic data critical for conservation. Wood turtles (Glyptemys insculpta) are declining across much of their range, yet baseline information is lacking in Michigan’s Lower Peninsula. To fill that gap, we performed standardized visual mark–recapture surveys at 27 one-kilometer river reaches in two distinct watershed systems during springs of 2022 and 2023. Using hierarchical N-mixture models with negative binomial mixtures to account for imperfect detection, we estimated site-level latent abundance and evaluated …

Dairy And Food Science Student Newsletter, August 28, 2025, Department Of Dairy And Food Science

Dairy and Food Science Student Newsletter

No abstract provided.

An Antioxidant Approach To Ameliorate The Effects Of Radiation And Chemotherapy On Cognition, Raymond Anan Otoo

Theses and Dissertations

Acute Lymphoblastic Leukemia (ALL) is a hematologic malignancy that primarily affects children and young adults. While advancements in treatment—including chemotherapy and cranial irradiation—have significantly improved 5-year survival rates in children to nearly 90%, radiation-induced cognitive impairment (RICI) remains a persistent and debilitating side effect. The brain, particularly the hippocampus, is highly susceptible to radiation-induced oxidative stress and inflammation. In the treatment of ALL, the combined use of chemotherapy and radiotherapy contributes to a heightened oxidative burden, exacerbating the risk of cognitive dysfunction. Although previous studies have linked radiation exposure to neurocognitive decline, the molecular mechanisms underlying RICI and chemotherapy-induced cognitive …

Dna Double-Stranded Breaks, A Hallmark Of Aging, Defined At The Nucleotide Resolution, Are Increased And Associated With Transcription In The Cardiac Myocytes In Lmna-Cardiomyopathy, Benjamin Cathcart, Sirisha M Cheedipudi, Leila Rouhi, Zhongming Zhao, Priyatansh Gurha, Ali J Marian

Faculty, Staff and Student Publications

Aims: An intrinsic feature of gene transcription is the formation of DNA superhelices near the transcription bubble, which are resolved upon induction of transient double-stranded breaks (DSBs) by topoisomerases. Unrepaired DSBs are pathogenic as they lead to cell cycle arrest, senescence, inflammation, and organ dysfunction. We posit that DSBs would be more prevalent at the genomic sites that are associated with gene expression. The objectives were to identify and characterize genome-wide DSBs at the nucleotide resolution and determine the association of DSBs with transcription in cardiac myocytes.

Methods and results: We identified the genome-wide DSBs in ∼1 million cardiac myocytes …

Alterations In Ether Lipid Metabolism In Obesity Revealed By Systems Genomics Of Multi-Omics Datasets, Yvette Schooneveldt, Sudip Paul, Kevin Huynh, Habtamu B. Beyene, Natalie A. Mellett, Gerald F. Watts, Joseph Hung, Jennie Hui, John Beilby, John Blangero

Human Genetics Publications

Ratios between two metabolites are sensitive indicators of metabolic changes. Lipidomic profiling studies have revealed that plasma ether lipids, a class of glycero- and glycerophospho-lipids with reported health benefits, are negatively associated with obesity. Here, we utilized lipid ratios as surrogate markers of lipid metabolism to explore the processes underlying the inverse relationship between ether lipid metabolism and obesity. Plasma lipidomics data from two independent human cohorts (n = 10,339 and n = 4,492) were integrated to assess the associations between 82 lipid ratios and obesity-related markers in males and females. Results were externally validated using mouse transcriptomics data …

The Immunophenotypic And Genetic Characterization Of Pediatric T -L Ymphoblastic Leukemia With A Mature Immunophenotype, Mahsa Khanlari, Wei Wang, Parastou Tizro, Mohammad K Eldomery

Faculty, Staff and Student Publications

Not available.

Adams Contribute To Triple Negative Breast Cancer Via Mtorc1 Pathway: Targeting Adam-Mtor Axis Improves Efficacy, Shuying Liu, Huiqin Chen, Mihai Gagea, Lorenzo Federico, Fan Zhang, Javier Gomez, Kim-Anh Do, William F Symmans, Gabriel N Hortobagyi, Gordon B Mills, Ana M Gonzalez-Angulo, Debasish Tripathy

Faculty, Staff and Student Publications

Breast cancer is the most frequently diagnosed cancer globally and the second leading cause of cancer-related deaths in American women. Triple-negative breast cancer (TNBC) lacks estrogen receptor, progesterone receptor, and human epidermal growth factor receptor 2. Thus, fewer targeting therapies are available for this most aggressive subtype. The A Disintegrin and Metalloproteinase (ADAM) family plays a vital role in cancer pathophysiology. Previous studies focused on single ADAM members. However, none of these have entered into the clinical arena as diagnostics or therapeutics for breast cancer. In this study, we demonstrate the upregulation of a panel of ADAM members in TNBC, …

Rna Sequencing-Based Transcriptome Analysis Of Liver In Laying Hens Supplemented With Dietary Probiotic Bacillus Species And Prebiotic Yeast (Saccharomyces Cerevisiae) Cell Walls, Ala E. Abudabos, Zafar M. Hakami, Ali R. Al Sulaiman, Riyadh S. Aljumaah, Valentino Palombo, Mashael R. Aljumaah, Mariasilvia D’Andrea, Abdulrahman S. Alharthi, Rashed A. Alhotan

Food and Animal Sciences Faculty Research

To investigate the impacts of dietary Bacillus-based probiotics and yeast-derived prebiotics on the hepatic transcriptome profile, 500 Hisex White laying hens were randomly allotted into five dietary treatments from 37 to 52 weeks of age: control; control + Bacillus subtilis; control + Bacillus subtilis and Bacillus licheniformis; control + Bacillus coagulans; and control + Saccharomyces cerevisiae yeast cell wall. Transcriptome analysis revealed a substantial number of differentially expressed genes exclusively between the control and prebiotic groups, identifying 2221 genes (FDR ≤ 0.05), with 980 genes upregulated (log2 fold change 0.69 to 24.62) and 1241 downregulated (log2 fold change −0.74 to …