Life Sciences Commons^™

Genetics Of Aluminum Tolerance And Diversity In United States Vigna Unguiculata Germplasm, Max Carl Miller

Tennessee State University Alumni Theses and Dissertations

Cowpea (Vigna unguiculata L. Walp) is an annual legume of immense importance across the world. However, knowledge of its ability to tolerate aluminum (Al) at toxic levels is not as comprehensive. This stress is an issue globally and especially in the southeastern United States, which has many Al-toxic soils. Here we determined multiple cultivars both tolerant and susceptible to Al toxicity. This was expounded upon by employing RNA sequencing on two cultivars from the United States with significantly different tolerance levels to characterize genes and pathways associated with Al toxicity and response. Multiple well-established gene orthologs like STOP, STAR, and …

Exploring The Autofermented Cyanobacterial Crude Extract As Antimicrobial Animal Feed Additives., Mohana Krishnan Neelakrishnan

Tennessee State University Alumni Theses and Dissertations

Overexploitation of antibiotics in the animal industry has driven the need for reduced usage, but this reduction has intensified the challenge posed by Salmonella enterica and Campylobacter jejuni to livestock systems and public health. This situation underlines the need for sustainable antimicrobial alternatives. Although cyanobacteria are promising in this respect, since they may produce a variety of bioactive metabolites, the amounts of these metabolites in their natural state are insufficient to elicit potent antibacterial action. In this study, autofermentation was investigated as a means of enhancing antibacterial efficacy in Fischerella ambigua and Nostoc muscorum by promoting the synthesis of organic …

Acute Effects Of Multi-Joint Eccentric Exercise On Lower-Extremity Muscle Activation Measured During Land And Water Walking, Brayden Worley, Brennan J. Thompson, Jon Carey, Talin Louder

Kinesiology and Health Science Student Research

Falls are a leading cause of injury and loss of independence in older adults, often linked to deficits in lower-limb muscle function and gait mechanics. Eccentric exercise can improve muscular resilience, while aquatic walking offers a safe, supportive environment to retrain gait; however, little is known about how these modalities interact at the neuromuscular level. This study compared lower-limb muscle activation during gait on land and in water, before and after an acute bout of eccentric exercise, in healthy young adults. Surface electromyography was collected from the tibialis anterior (TA), gastrocnemius medialis (GM), vastus lateralis (VL), and biceps femoris (BF) …

The Chemistry Of Color: A Comparative Analysis Of Natural And Synthetic Dyes In Textile Application, Caitlyn Pinaga Cpht

Honors Program Theses and Research Projects

The Chemistry of Color: A Comparative Analysis of Natural and Synthetic Dyes in Textile Application examines how color functions at the intersection of chemistry, culture, and environmental responsibility. This project traces the evolution of dyeing from ancient natural pigment traditions, rooted in ecological knowledge, agricultural practices, and cultural identity, to the industrial rise of synthetic dyes, whose laboratory precision enabled unprecedented consistency, brightness, and global textile expansion. Through experimental swatch analysis, the study compares the behavior of natural dyes derived from plants and food sources with modern fiber-reactive and synthetic dye classes on a range of fibers including cotton, bamboo …

Time Series Modeling Of Land Use And Land Cover Classification Using Remote Sensing, Ahmed A. F. Abd El Hamed, Fawzi H. Zarzoura, Mahmoud El-Mewafi

Mansoura Engineering Journal

Understanding land use / land cover changes is essential for planning sustainable development. It represents the transformation of the land due to human activities, which is related to sustainable development that aims to achieve the needs of the present generations without affecting the future. Land use / Land cover is studied in Al Alamein region, northern Egypt, with supervised classification methods using satellite images from Landsat 8 and Sentinel - 2A in 2023 and analyzes the accuracy assessment, which shows Maximum likelihood is better in both sensors achieving overall accuracy (91.80% and 91.02%) and kappa coefficient (0.814 and 0.8799). Then …

Evaluating The Sna1 Gene Using Crispr Mediated Knockdown In Ewing Sarcoma Cells, Rosanna Jees, Sonia Cerrillo, Terry Jo Shackleford

Mechanisms of Disease

Ewing sarcoma is a pediatric cancer with limited therapeutic options and poor long-term survival. CRISPR–Cas9–mediated gene editing provides a powerful tool for investigating tumor molecular behavior and identifying potential therapeutic targets. In this study, we worked with CRISPR–Cas9 in Ewing sarcoma ES8 cells to evaluate the roles of the transcription factors SNAI1 and SNAI2, both implicated in epithelial–mesenchymal transition and cancer progression. Using guide RNAs targeting each gene, we assessed proliferation, apoptosis, migration, and long-term survival through Incucyte live-cell imaging and colony-formation assays. Knockout of SNAI1 resulted in decreased cell proliferation and reduced migratory capacity compared with controls, suggesting a …

Effects Of Ptk2 Knockdown On Key Cellular Processes In Es8 Cells, Adrian Cruz, Emiliano Rueda, Dylan Andrews, Terry Jo Shackleford

Mechanisms of Disease

Ewing sarcoma is an aggressive form of pediatric cancer characterized by rapid growth and metastatic potential, with limited treatment options available. PTK2 is a key regulator of many pathways involved in tumor behavior, including integrin-mediated adhesion, survival, and migration. This research lab investigated how CRISPR-Cas9 knockdown of PTK2 can affect cellular processes in ES8 Ewing sarcoma cells. To assess the effectiveness of reducing PTK2 expression, we utilized live-cell imaging, wound-healing assays, measurements of caspase activity, colony formation, and qRT-PCR. Our results showed that incomplete knockdown decreased cell proliferation and colony formation, while also increasing apoptotic activity. Additionally, migratory ability was …

How Cxcr4 Knockdown Affects Tumorigenic Properties In Es8 Cells, Gabriella Galdeano, Damon James, Terry Jo Shackleford

Mechanisms of Disease

C-X-C chemokine receptor type 4 (CXCR4) is a seven-transmembrane G-protein–coupled receptor (GPCR). When activated by its ligand SDF-1 (CXCL12), CXCR4 triggers signaling pathways that promote cell survival, proliferation, angiogenesis, and chemotaxis. Many cancers exploit the CXCR4/SDF-1 axis to support tumor growth and metastasis. Ewing sarcoma (ES8) is an aggressive pediatric bone and soft-tissue cancer, and elevated CXCR4 signaling has been linked to increased migration and metastatic behavior. ES8 cells, a well-established ES8 cell line, provided a model to study how CXCR4contributes to tumor progression. CRISPR-Cas9 genome editing was used to knock down CXCR4 in ES8 cells, delivered through lipid-based transfection. …

Investigating The Role Of Twist1 In Cancer Progression Using Crispr Knockdown, Abby Guerrero, Sofia Perez, Terry J. Shackleford

Mechanisms of Disease

TWIST1 is a transcription factor that plays a critical role in epithelial-mesenchymal transition (EMT), a process that promotes cancer cell migration, invasion, and metastasis. This study investigates the functional role of TWIST1 in cancer progression by using CRISPR/Cas9-mediated knockdown to examine its effects on apoptosis, proliferation, migration, and invasion. TWIST1 was knocked down in cancer cell lines using CRISPR/Cas9 assay. Knockdown efficiency and changes in genes were confirmed by qRT-PCR. Functional assays included caspase assays for apoptosis, MTT assays for proliferation, wound-healing assays for migration, Matrigel invasion assays, and colony formation assays to assess long-term growth and invasive potential. Across …

Itgb1 Knockdown Alters Apoptosis And Proliferation In Ewing Sarcoma Cells, Aditi Kumar, Magdalena Saad, Terry J. Shackleford

Mechanisms of Disease

Integrin beta 1 (ITGB1) is an adhesion receptor that link cells to the extracellular matrix and regulates pathways involved in survival, proliferation, and migration. In order to understand its role in Ewing Sarcoma, we applied CRISPR-Cas9 with two guide RNAs to knock down ITGB1 in ES8 cells. We then assessed changes in cell growth, death, movement, and gene expression using Incucyte live-cell imaging, colony formation assays, wound healing assays, and qRT-PCR. qRT-PCR showed partial ITGB1 reduction, with ITGB1_gRNA2 producing the strongest decrease. ITGB1_gRNA2 also led to a small increase in caspase activity and a 20-30% increase in proliferation at the …

Microplastic Exposure Alters Tubulin Leading To Alterations In Insulin Secretion By Pancreatic Beta Cells, Anakaren Garcia

Honors Program Theses and Research Projects

Abstract

Diabetes mellitus is a metabolic disorder characterized by elevated blood glucose levels due to impaired insulin secretion or diminished cellular response to insulin (resistance). Pancreatic β-cells depend on a well-organized cytoskeleton, particularly microtubules composed of α- and β-tubulin heterodimers, to transport insulin granules to the plasma membrane during glucose-stimulated insulin secretion (GSIS). Disruption of microtubule organization can impair granule trafficking and reduce insulin release. However, emerging environmental toxicants such as microplastics that interfere with β-cell cytoskeletal function remains largely unexplored.

Microplastics (MPs) are small plastic particles (< 5 mm) that have become widespread environmental contaminants and can enter biological systems. Previous studies have shown that MPs can induce cellular stress and protein misfolding, but their effects on microtubules in pancreatic β-cells are not well characterized. This study investigates whether exposure to fluorescent polystyrene microplastics alters microtubule organization and insulin secretion in βTC-6 cells.

βTC-6 cells were treated with 10 µg/mL of fluorescent polystyrene microplastics for …

Predicting, Culturing, And Characterizing Beneficial And Detrimental Strains Associated With Plant Phenotypes., Anastasiia Onyshchenko

Arts & Sciences Graduate Student Theses and Dissertations

Modern agriculture’s reliance on chemical fertilizers has greatly increased crop yields but has also contributed to soil degradation, environmental pollution, and unsustainable resource use. Plant growth–promoting rhizobacteria offer a sustainable alternative, enhancing plant growth through mechanisms such as nitrogen fixation, phosphate solubilization, siderophore production, and phytohormone modulation, while also protecting plants against pathogens and abiotic stresses. However, soil microbial communities are highly diverse and structurally complex, making the isolation of individual beneficial strains challenging. Traditional approaches rely on selective media and labor-intensive phenotypic screening, which often capture only a small fraction of soil diversity. To overcome these limitations, we combined …

Oregon State Rank Assessment For Plumed Clover (Trifolium Plumosum Var. Plumosum), Nora Dunkirk

Institute for Natural Resources Publications

Oregon state conservation status assessment for Plumed Clover (Trifolium plumosum var. plumosum) using NatureServe methodology, 2025.

High School Science Experience For Students With Cognitive Disabilities: A Pilot Study, Cassandra Wychers

Honors Theses

Although education has become more adaptive, inclusive of all students who learn differently including for people with cognitive disabilities, closer examination may reveal that high school science classrooms are still challenging learning spaces. Science is an important part of the school curriculum as it teaches students about engaging in the world around them while also encouraging skills involving evidence, inquiries, and critical thinking. All students are entitled to learn science in their classrooms even if they have a cognitive disability (Apanasionok, et al. 2019). Although there are many complexities to defining disability in the world and in education, this study …

Seed Amplification Of Msa Alpha-Synuclein Aggregates Preserves The Biological And Structural Properties Of Brain-Derived Aggregates, Fei Wang, Victor Banerjee, Carla Barria, Santiago Ramirez, Tyler Allison, Damian Gorski, Haley Evans, Quynh Nguyen, Danielle Harrison, Rabab Al-Lahham, Nicole De Gregorio Carbonell, Michelle Pinho, Sanne Kaalund, Jonas Folke, Susana Aznar, Luis Concha-Marambio, Mohd Ishtikhar, Venkata Kps Mallampalli, Sandra Pritzkow, Mohammad Shahnawaz, Matthew L Baker, Irina Serysheva, Claudio Soto

Faculty, Staff and Student Publications

Parkinson's disease (PD), Dementia with Lewy bodies (DLB), and multiple system atrophy (MSA), are characterized by the misfolding and aggregation of alpha-synuclein (αSyn). Compelling evidence showed that αSyn aggregates exist as distinct conformational strains in different synucleinopathies. Recently, we reported that the αSyn Seed Amplification Assay (αSyn-SAA) can amplify and distinguish αSyn strains from PD and MSA. In this study, we investigate whether MSA-seeded, SAA-amplified αSyn fibrils retain the biological and structural properties of the αSyn seeds present in MSA brains. We study the biological activities of both brain-derived and SAA-amplified αSyn aggregates using an αSyn "biosensor" cell model and …

Patent Searching With Uspto, Derwent Innovation And Lens.Org, Ibis Anette Moreno-Lozano

Day Family Research Lab Workshop Series

No abstract provided.

Disentangling Complex Relationships And Disjunctions In Western Camassia: Integrating Multiple Criteria To Resolve Taxonomic Boundaries, Susan R. Kephart, Theresa M. Culley, Jenny K. Archibald, Robert Tunison, Kathryn E. Theiss, James E. Kephart

Michigan Tech Publications

Premise: Understanding genetic and morphological variability helps efforts to sustain landscapes and develop effective species concepts for resolving difficult groups. To unravel puzzling relationships and range disjunctions, we applied morphometrics, phenology, phylogenetics and population genetics in Camassia species with cultural, ecological, and conservation value, asking: Do the unusual Camassia populations in northeastern California represent previously unrecognized, disjunct C. howellii? Do C. howellii, C. leichtlinii, and C. quamash maintain diagnostic features in allopatry or sympatry? Are C. quamash subsp. breviflora and subsp. linearis taxonomically distinct?. Methods: We evaluated 34 Camassia populations in situ for morphometric traits, phenology, and habitat type, collecting …

Ifnγ-Driven Skewing Towards Th1 Over Th17 Differentiation Underlies Crs And Neutropenia In Car-T Therapy, Payal Goala

USF Tampa Graduate Theses and Dissertations

CAR-T therapy has improved overall and complete response rates, andprolonged progression free survival of patients with relapsed/refractory hematological malignancies. The short-term and long-term impacts of anti-CD19 CAR-T products on the immune landscape of B cell malignancies has garnered considerable attention in recent years. Most notably, a subset of patients experience high-grade cytokine toxicities, including cytokine release syndrome and immune cell-associated hematologic toxicity that manifest as prolonged cytopenia. Whereas etiology of CRS is more well established, the mechanisms leading to CAR-T associated cytopenia remains elusive. Rejeski et. al. showed that severe cytopenia in patients post CAR-T treatment led to frequent infections …

Retrospective Review Of Metastatic Hormone Receptor-Positive Inflammatory Breast Cancer Patients Reveals Poor Responses To Cyclin Dependent Kinase 4/6 Inhibition, Azadeh Nasrazadani, Rebecca S Tidwell, Megumi Kai, Bora Lim, Vicente Valero, Debu Tripathy, Sadia Saleem, Bisrat G Debeb, Anthony Lucci, Wendy A Woodward, Rachel M Layman

Faculty, Staff and Student Publications

Background: Patients with inflammatory breast cancer (IBC) have aggressive biology and relatively inferior responses to standard-of-care (SOC) therapies. Understanding the efficacy of SOC therapies in IBC is critical to optimize outcomes. Our objective was to assess the progression-free survival (PFS) of metastatic hormone receptor-positive HER2-negative/low (HR+HER2-) IBC patients treated with CDK4/6 inhibitors (CDKIs) and hormonal therapy (HT).

Methods: Data from 58 IBC patients with metastatic HR + /HER2- IBC from a single institution were reviewed. The medians (95% confidence intervals) of overall survival (OS), PFS, and time on treatment (ToT) from the time of CDKI initiation were reported via the …

Development Of Microcrystalline Tests For Fentanyl, Clonazepam, And Diazepam Identification, Karis French

Honors Projects

Microcrystalline tests are chemical tests that involve the mixing of a substance with a specific reagent resulting in the formation of a crystal structure. Currently, microcrystalline tests are used as presumptive tests in forensic drug chemistry laboratories to provide the suspected identity of unknown substances. There is, however, a limited amount of research on this use of microcrystalline structures. Additionally, this existing research is outdated and does not include some of the newer commonly encountered controlled substances including fentanyl, clonazepam, and diazepam. This literature-based review attempts to identify reagents that could be used in microcrystalline tests for the identification of …

White Nose Syndrome In Bats And Infant Mortality In Georgia, Rhys Medcalfe, Sean Medcalfe, Simon Medcalfe

Journal of the Georgia Public Health Association

Background: White nose syndrome (WNS) is a disease in bats caused by the fungus Pseudogymnoascus destructans that can result in bat mortality rates of over 90%. WNS was discovered in Georgia in 2012 in 3 counties but has continued to spread to 14 counties as of 2023, with P. destructans detected in a further seven. Biodiversity loss has been linked to human health outcomes including infant mortality. Understanding the link between WNS, biodiversity loss, and infant mortality in Georgia is important because Georgia ranks 42^nd out of the 50 states for infant mortality rate.

Methods: Using panel data from …

Elucidating The Mechanism Of Neprilysin-Regulated Triple-Negative Breast Cancer Invasion, Kai Lam, Ellie Griner

Student Scholar Symposium

Triple-negative breast cancer (TNBC) is a highly invasive and difficult-to-target form of cancer. It is characterized by a lack of estrogen receptors, progesterone receptors, and human epidermal growth factor 2 receptors, making current targeted therapies ineffective and leaving chemotherapy as the primary treatment option. Previous studies in this lab have linked reduced expression of neprilysin (NEP), a membrane-bound protease that cleaves and inactivates mitogenic peptides, to a highly invasive phenotype in TNBC. Our data indicates that while NEP promotes phosphoinositol 3-kinase (PI3K) signaling, it simultaneously inhibits cellular invasion. Here, we begin to explain a possible mechanism behind the anti-invasive role …

Dietary Supplementation In Lambs: Evaluating The Effects Of Depth Charge On Meat Composition, Alianna Koesel

Student Scholar Symposium

Often, animals destined for consumption are fed supplements to help speed up or slow down their growth rate, enhance aesthetics, or standardize the product. Herein, we examined the supplement Depth Charge and its effects on the fat and protein content in lambs of the same age and lineage. Depth Charge increases fiber consumption to add spring of rib or an outward rib cage curvature to an animal's appearance while restricting feed consumption. Since the supplement targets the rib-loin area, we chose to analyze loin chop, loin roast, and hamburger cuts from each lamb and conducted experiments to determine how each …

Δ-Valerobetaine As A Novel Inhibitor Of Breast Cancer Cell Migration: Hinders Cellular Migration Of Mcf-7 Cells Through Reducing Atp Concentrations And Fak Activation, Braxton Ivie, Lincoln Brown, Bryson Lovorn

Student Scholar Symposium

δ-valerobetaine as a Novel Inhibitor of Breast Cancer Cell Migration: Hinders Cellular Migration of MCF-7 Cells Through Reducing ATP Concentrations and FAK Activation

Lincoln Brown, Braxton Ivie, Bryson Lovorn, Joshua Owens

Based on female breast cancer diagnoses from 2014-2020, the CDC reports that the 5-year relative survival rate for local and regional breast cancers was 98.9% and 86.3%, respectively, while the survival rate for metastasized cancers was reportedly 32.4%. These discrepancies in survival highlight an increased mortality rate when cancer metastasizes. Interestingly, aggressive tumors have been shown to metabolize fats at higher rates than low-metastatic tumors. Recently, our group has …

Exploring The Role Of Faecalibacterium Prausnitzii Metabolites In Modulating Fatty Acid Metabolism And Protecting Hepg2 Cells During Induced Steatosis, Morgan Martin, Emerald Cole

Student Scholar Symposium

The obesity epidemic has risen to astronomical numbers with over 650 million adults being classified as obese (BMI≥30). Obesity is of critical concern as excess body fat storage is a comorbidity to various diseases including type-2 diabetes, cardiovascular disease, and cancer. Although obesity has classically been attributed to genetics and unhealthy lifestyle choices, there is a growing interest in other factors affecting obesity, including the gut microbiome. For example, mice lacking a microbiome are resistant to weight gain even when placed on a high-calorie diet. It can then be concluded that bacteria and the metabolites they produce can dramatically affect …

Effect Of Ovarian Characteristics And Propylene Glycol Supplementation On Pregnancy Rates Per Ai In Suckled Beef Cows Subjected To A 7-Day Co-Synch Fixed-Time Ai Protocol Across Seasons, Body Condition Scores, And Postpartum Intervals, Pachara Pearodwong, Sarawanee Khunmanee, Kajohn Nitiwararuk, Thuwanon Boonkerd, Thananon Junsuri, Andrew A. Ponter, Winai Kaewlamun

The Thai Journal of Veterinary Medicine

The present study evaluated the independent effects of ovarian characteristics and oral propylene glycol (PG) supplementation on pregnancy rates in suckled beef cows subjected to the 7-day CO-Synch + CIDR fixed-time AI (FTAI) protocol across different seasons, body condition scores (BCS; 5-point scale), and parturition-to-FTAI intervals. Cows received a CIDR insert and 10 μg GnRH on day -10, followed by 25 mg PGF2α at CIDR removal on day -3, and another 10 μg GnRH 72 h later (day 0). In Study 1, the ovarian characteristics of 36 cows were assessed transrectally on days -10 and 0 relative to the FTAI. …

Temperature Predicts Myotis Lucifugus Declines And Persistence After White-Nose Syndrome Introduction, Tanner M. Barnes, Jared D. Wolfe, Allen Kurta, Steve M. Smith, John E. Depue

Michigan Tech Research Data

No abstract provided.

Book Of Abstracts, Seton Hall University

2025 Augustine Undergraduate Research Symposium

No abstract provided.

Interview With Orca Award Winner John J. Janovy, Jr., Unl Biological Sciences; Parasitologist And Author, Mark Griep, John J. Janovy Jr.

UNL ORCA Interview Series

Professor John Janovy, Jr., was awarded the ORCA in 1998. John was born in Louisiana, but his family moved to Oklahoma, where he grew up in Tulsa and then Oklahoma City. Something in his youth must have sparked an interest in science because he earned three degrees from the University of Oklahoma in Norman: a bachelor of science in math in 1959, a master of science in zoology in 1962, and doctoral degree in zoology in 1965. Next, he ventured 1400 miles east to Rutgers University in New Brunswick, New Jersey, where he did post-doctoral work for about a year. …

Identifying Stable Parental Lines For Stripe Rust Resistance In Soft Red Winter Wheat, Ehsan Shakiba, David Moon

Crop, Soil and Environmental Sciences Faculty Publications and Presentations

Stripe rust, caused by Puccinia striiformis f. sp. tritici, continues to be a major challenge in soft red winter wheat (SRWW) production, especially in the southern United States. In this area, mild winters and wet springs frequently lead to early and severe disease outbreaks. Although many lines demonstrate resistance in a single season, that resistance often does not last across multiple years. For breeding programs, this variability makes it hard to identify truly reliable lines. In this study, we evaluated 111 SRWW breeding lines over three consecutive growing seasons (2021-2024) under artificial inoculation in field conditions in Fayetteville, Arkansas. Stripe …