Life Sciences Commons^™

Development Of The Core Lit Apparatus For Seaweed Hatcheries (Clash) Bioreactor Using A Germplasm Approach, Phillip O. Tahimic

Cal Poly Humboldt theses and projects

In comparison to the Asian aquaculture industry, the United States has minimal seaweed production. There are several drivers of the limited industry in the US, such as regulatory constraints and limited market demand, but the ability to scale is a considerable barrier to commercialization. One possible solution is the development of land-based systems that integrate bioreactors, which utilize fewer resources and do not wholly rely on an oceanic flow-through system. This study assessed the application of a modular bioreactor, known as Core Lit Apparatus for Seaweed Hatcheries (CLASH), as a nursery system and open-source project designed to expand land-based seaweed …

Bacterial Community Function In Pitcher Plant Leaves As An Experimental And Mathematical Model Of Functional Dynamics, Amelia Jackson

Cal Poly Humboldt theses and projects

This work looks at two different sites with differing abiotic factors in measures of altitude, temperature and precipitation. The site with higher altitude, larger temperature range, and lower precipitation, located in Plumas National Forest (Quincy, CA), had a flat BEF trend that suggests functional redundancy The site with lower altitude, more moderate temperatures, and higher precipitation located in Six Rivers National Forest (Gasquet, CA), also had a flat BEF trend. These abiotic factors could correlate to the significantly different bacterial compositions between sites. The second portion of this work is modeling the host antagonist relationship between Eyxra moths and their …

Integrating Multi-Method Monitoring To Characterize Seasonal Habitat Use Of Green Sturgeon (Acipenser Medirostris) In Humboldt County, California, Olivia S. Boeberitz

Cal Poly Humboldt theses and projects

Green sturgeon (Acipenser medirostris) are a long-lived, anadromous, iteroparous species that spawns in large West Coast North American rivers but ranges widely in the coastal ocean. For coastal California Native American Tribes, green sturgeon are a vital part of their cultural heritage and traditions, and central to their food security. Two genetically distinct population segments (DPSs) of green sturgeon are recognized in the United States, Canada, and Mexico; the southern DPS is listed as “threatened” under the Endangered Species Act and the northern DPS is considered of “conservation concern.” Although green sturgeon are known to use large estuaries …

Depth And Soil Physical Context Govern Soil Organic Carbon, While Woody Cover Moderates Native Vegetation Relationships In California Rangelands, Dabid Zuniga Garcia

Cal Poly Humboldt theses and projects

Soil organic carbon (SOC) is central to soil function and an important component of climate mitigation in rangeland ecosystems, but the controls on SOC across California’s working rangelands remain understudied. Using data from Point Blue Conservation Science’s Rangeland Monitoring Network, this study evaluated how soil properties, vegetation structure, and recent climate relate to SOC concentration at depths of 0–10 cm and 10–40 cm across 145 ranches spanning four California regions. Linear mixed-effects models showed that SOC was consistently higher in topsoil than subsoil and highest in coastal sites relative to interior valleys. Bulk density was the strongest negative predictor of …

Evidence Of Personality And Movement Syndromes In A Mobile Predator: How Individual Hunting Strategy Shapes Ecosystem Service Potential By Barn Owls (Tyto Furcata) In An Agroecosystem, Lauren M. Jackson

Cal Poly Humboldt theses and projects

Animal-mediated ecosystem services are delivered through the behavior of individuals, yet most studies evaluate service-providing species at the population level. We tested whether individual variation in plumage, behavior, and movement predicted hunting behavior and vineyard habitat use by adult male American barn owls (Tyto furcata) nesting in winegrape vineyards in Lodi, California, USA. We fitted GPS-accelerometer tags to 42 breeding males, quantified breast-plumage traits, measured neophobia using repeated novel object tests, and derived nightly movement metrics from GPS tracks and hidden Markov model-classified hunting locations. Several behavioral traits were repeatable, but movement traits showed the clearest repeatable individual …

Symmetry In Affiliative Interactions Of North American River Otters (Lontra Canadensis) In A Northern California Estuary, Charlotte E. Norman

Cal Poly Humboldt theses and projects

Patterns of participation in affiliative behaviors may reflect underlying social dynamics. Two affiliative behaviors, allogrooming and social play, were assessed for their symmetry, or relative participation of participants, in a wild group of North American river otters. Behavioral observations were conducted over five months at the Little River estuary in northern California using video recordings and all-occurrence sampling. Observations included all-adult groups, a family group consisting of a breeding female and two pups, and an extended family group with one or two helpers, indicative of cooperative breeding. Individuals engaged in allogrooming and social play were classified by their group membership, …

Proteome-Based Identification And Validation Of Nxpe3 In Childhood Acute Lymphoblastic Leukaemia, Najia Tabassum, Yamna Khurshid, Basir Syed, Aftab Ahmad, Sadia Muhammad, Rehan Imad, Talat Mirza

Pharmacy Faculty Articles and Research

Background: Childhood acute lymphoblastic leukaemia (cALL) tends to metastasize to central nervous system. Treatment with antileukemic agents against CNS leukaemia is an essential component for cure in ALL. Hence, it is essential to identify biomarkers for CNS infiltration. Proteomics, supported by mass spectrometry, is the platform for exploring biomarkers in various biological samples, contributing to translational research. Objectives: This study aimed to identify the plasma proteome profile of children across different risk groups of cALL. Neurexophilin and PC-esterase family, member 3 (NXPE3), was validated. The protein-protein interactions (PPI) of NXPE3 were evaluated with bioinformatics analyses. Methods: Plasma …

Food Recognition With Visual Language Models: Search Re-Ranking Or Retrieval-Augmented Generation?, Kian Yu Gan, Phuong Anh Nguyen, Chong-Wah Ngo

Research Collection School Of Computing and Information Systems

Despite the rapid advances in Visual Language Models (VLMs), these models struggle to recognize culture-specific food items. While VLMs are effective in recognizing popular cultural dishes, their performance is suboptimal for dishes that are unique but not widely known internationally. Specifically, VLMs often generate either generic labels or hallucinated names for dishes that are localized to a particular culture. As a result, retrieval-augmented generation (RAG), which retrieves relevant recipes as references for VLMs, emerges as a promising approach. Nevertheless, recipe retrieval, which is itself imperfect, could mislead VLMs into generating inaccurate or culturally inappropriate dish names. This paper presents a …

Metabolic Syndrome Beyond Diagnostic Criteria: Population-Scale Integrative Metabolomics Characterization, Marwa Talal

Theses and Dissertations

Background: Metabolic syndrome (MetS) is a complex cluster of interrelated metabolic abnormalities associated with elevated cardiometabolic risk. While diagnosis is based on well-established five clinical criteria, these may overlook early or atypical metabolic alterations. Large-scale metabolomic profiling offers an opportunity to identify biochemical signatures of MetS beyond diagnostic bias and to evaluate their relative importance across different presentations of the syndrome.

Methods: Data from 117,147 UK Biobank participants were analyzed in a cross-sectional design. High-throughput NMR quantified 75 circulating metabolites, for. Univariate analyses, MetS subtype stratification, and elastic net models with SHAP interpretation were applied to assess feature …

Data And Analysis Code For Agricultural Risk Perceptions And Willingness To Change Farming Practices In The Rio Grande Valley Of Texas, Owen Temby, Dongkyu Kim, Anthony R. Lima

Datasets

This dataset supports research on farmers’ perceptions of agricultural risk and their stated willingness to use water-management and conservation practices in the Rio Grande Valley of South Texas. Data were collected in 2024–2025 from farm owners in Cameron, Hidalgo, Starr, and Willacy counties. Mailed invitations directed recipients to a Qualtrics questionnaire, followed by telephone outreach through the UTRGV Center for Survey Research and Policy Analysis. Participants could respond online or answer the questionnaire orally during a telephone interview. The released dataset contains 93 records with recorded consent, including partial responses; 72 records support the core regression analyses. Survey measures cover …

Moth Assemblages Of Fort Leonard Wood, Missouri, Kimberly Anne Steese

Masters Theses

"Moths (Lepidoptera) are ecologically important insects that function as herbivores, pollinators, prey resources, and indicators of ecosystem integrity. Despite their ecological significance, moth communities in the Missouri Ozarks remain poorly documented, particularly within military-managed landscapes. This thesis examined patterns of moth diversity, assemblage structure, and environmental drivers at Fort Leonard Wood (FLW), Missouri, while also assessing the distribution and genetic diversity of species within the genus Papaipema.

Field surveys were conducted from 2019 through 2021 across prairie, savanna, forest, and restoration habitats at FLW using UV bucket traps, sheet trapping, and targeted larval surveys. Across all sampling efforts, 29,116 individual …

Acute Lower Leg Transcutaneous Electrical Nerve Stimulation Increases Leg Blood Flow And Exercise Capacity In Patients With Peripheral Arterial Disease, Thomas K. Pellinger, Catherine B. Neighbors, Joshua C. Hefta, Patrick H. Luo, Carl B. Suarez, Erotokritos T. Varlas, Grant H. Simmons, Brett J. Wong

EVMS School of Health Professions Faculty Publications

We tested the hypothesis that two different durations of lower leg transcutaneous electrical nerve stimulation (TENS) increase post-TENS popliteal artery blood flow (PABF), popliteal vascular conductance (PVC), and 6-min walk distance (6MWD) in patients with peripheral artery disease (PAD). Seven subjects (5 male, 2 female) with PAD (67.9 ± 7.4 years; claudication: ankle-brachial index (ABI) < 0.90) participated in three randomized treatment sessions: control or bilateral TENS (3.0 Hz burst mode, 250 µs) for either 15 or 45 min. PABF (Doppler ultrasound) was measured before and after treatment. 6MWD and time/distance to claudication onset were measured 25 min post-treatment during each session. PABF, PVC and 6MWD increased after both 15 and 45 min of TENS (P < 0.05, post-TENS vs. pre-TENS baseline/control). Our preliminary results suggest that as little as 15 min of lower leg TENS increases leg blood flow and exercise capacity in individuals with PAD.

Ecological And Evolutionary Divergence Of A Shared Sympatric Color Polymorphism In Paracirrhites Hawkfish, Samuel Greaves

Graduate Studies Theses and Dissertations 2026

Color polymorphism provides a tractable window into how selection, gene flow, and ecology interact to maintain phenotypic diversity and initiate divergence. I investigate the ecological and genomic basis of sympatric light and melanistic morphs in two reef hawkfishes, Paracirrhites arcatus and P. hemistictus, across Guam and Hawai‘i. This study aims to determine whether the color morphs of these hawkfishes are a stable polymorphism or have begun the process of ecological speciation. For P. arcatus revealed substantially lower density on Guam (0.086 ind/m²) than reported for Hawai‘i (0.292 ind/m²) and a strong skew away from the melanistic morph on Guam …

Club Sport Injury Rates, Injury Characteristics, Functional Movement Screen Relationships, And Impact Of Core Training, Rebecca Carson

Graduate Studies Theses and Dissertations 2026

It is estimated that over 10% of college students take part in club sports at their college or university, yet sport-related injuries are a common consequence of participation. The Functional Movement Screen™ (FMS™) is a movement analysis tool that identifies functional deficits and asymmetries and is widely applied in athletic settings to assess injury risk and inform rehabilitation and injury prevention programs. Core strength and stability play a crucial role in functional movement quality and may even reduce risk of injury and improve sport performance. This dissertation aimed to establish club sport injury rates and characteristics for club men’s ice …

Carbaryl Exposure Alters Gut Microbiome Composition Without Affecting Morphometrics In Eastern Purple Martin (Progne Subis Subis) Nestlings, Stephanie A. Gaspar

Graduate Studies Theses and Dissertations 2026

Eastern purple martins (Progne subis subis) rely entirely on human-provided nesting structures throughout their breeding range in eastern North America. To control haematophagous mites that commonly infest these nests, many landlords apply the carbamate insecticide, carbaryl. Although effective at reducing ectoparasite populations, carbaryl exposure may have unintended effects on the gut microbiome, growth, and condition of developing birds. This study examined the effects of carbaryl exposure on gut microbial communities and morphometric condition in wild nestlings. Nests were experimentally treated with carbaryl, and nestling morphometrics and fecal microbiomes were compared between treated and untreated groups at 18 days …

Exercise-Induced Muscle Swelling And Impedance Changes Of The Elbow Flexor, Sofea Smith

Graduate Studies Theses and Dissertations 2026

Electrical impedance myography (EIM) is a non-invasive method that assesses body fluid distribution using impedance (Z), resistance (R), reactance (Xc), and phase angle (PhA). These values have previously been used to interpret cellular health and fluid shifts. Acute muscle edema known as a “pump,” has been reported during and after resistance training exercise and the extent to which bioelectrical properties of the muscle tissue are affected during this process remains relatively unexplored. Therefore, the purpose of this study was to assess changes in EIM during exercise-induced muscle swelling via resistance training. Thirty-one resistance-trained adults (male=21, female=10; age=23.4±6.5 years, BMI=26.17±3.04 kg/m2) …

Regulation And Localization Of Urease In Sporosarcina Pasteurii: Implications For Coastal Protection, Amar Kosovac

UNF Graduate Theses and Dissertations

Coastal erosion is a persistent problem exacerbated by global warming. As the climate changes, the southeastern coast is likely to experience an increased frequency and intensity of storms, which can significantly damage the coastline. The coast is crucial as it serves as a habitat for numerous animals, supports tourism, and underpins infrastructure. Microbially induced calcite precipitation (MICP) is a novel method to enhance erosion resistance in sandy soils using the non-pathogenic bacterium Sporosarcina pasteurii. S. pasteurii produces urease to catalyze urea into bicarbonate, which drives the precipitation of calcium carbonate. Maximal expression of urease is a limiting factor for …

Assessing The Effects Of Theta Transcranial Alternating Current Stimulation During Working Memory Performance On Theta-Gamma Phase Amplitude Coupling In Healthy Adults, Samantha M. Zimmer

UNF Graduate Theses and Dissertations

Transcranial alternating current stimulation (tACS) is an emerging form of noninvasive brain stimulation with substantial effects on behavior and brain function due to its ability to entrain endogenous neural oscillations. Working memory is a cognitive process that involves holding onto information that is no longer present in the environment and manipulating or reorganizing information for immediate use. Theta oscillations are critical for the functionality of the frontoparietal network and are involved in strengthening communication and coordination between frontal and parietal brain regions, especially during working memory processing. TACS applied at the theta frequency, targets theta oscillations, strengthens network communications, and …

Cryptic Social Mechanisms And Associated Consequences In The Gouldian Finch, Thomas Mateo

UNF Graduate Theses and Dissertations

Many species of birds rely on visual displays to communicate information to their conspecifics, but it can be difficult to determine the specific functions of such displays. In the Gouldian Finch and its relatives, the contexts in which the peering display has been witnessed indicate that it has multiple uses in social learning such as song learning and communicating reproductive intent. Two flocks of finches were housed separately at the University of North Florida aviary and observed for several months while nest boxes were placed in each habitat. In both flocks, peering increased during the summer molt, but did not …

Site Use And Demographic Changes In A Northwest Atlantic Sea Turtle Foraging Ground, Ryan C. Welsh

Graduate Studies Theses and Dissertations 2026

and site fidelity, making mortality difficult to distinguish from redistribution. We developed a behaviorally explicit framework to separate true mortality from changes in availability caused by movement and redistribution. We evaluated this problem for juvenile green turtles (Chelonia mydas) and loggerhead turtles (Caretta caretta) captured at the St. Lucie Nuclear Power Plant intake canal on Florida’s Atlantic coast using a multi-decade capture–mark–recapture record spanning 1977 - 2023. Hidden Markov Models classified individuals into latent habitat-use strategies (Nomadic, Resident, and Transient) based on capture timing and body size. Across both species, these strategies captured consistent differences in site fidelity and recapture …

Interactions Of Complement With Paramyxovirus Persistently Infected Cells, Nasser N. Yousef

Graduate Studies Theses and Dissertations 2026

Persistent RNA virus infections (PI) are often characterized by extended viral shedding and maintained cycles of inflammation. We have an incomplete understanding of interactions between exogenous and cell intrinsic Complement (C') factors and paramyxovirus PI cells. Parainfluenza virus 5 (PIV5) acute infected (AI) cells can be recognized and lysed by exogenous C’ factors, but PI cells become resistant to C’-mediated lysis. We hypothesized that the relative sensitivity of AI and PI cells to C’ lysis is correlated with viral glycoprotein expression on the surface of cells. PI cells display lower levels of viral glycoprotein expression compared to AI cells. Pharmacological …

Evaluating Novel Targeted Combination Therapies For Gastrointestinal Cancers, Joseph A. Goode

Graduate Studies Theses and Dissertations 2026

Gastrointestinal (GI) cancers remain among the leading causes of cancer-related mortality worldwide, with therapeutic resistance continuing to limit the effectiveness of current treatments. Three complementary studies were conducted to identify therapeutic vulnerabilities and evaluate novel combination strategies in pancreatic ductal adenocarcinoma (PDAC) and colorectal cancer (CRC).  First, dual inhibition of fibroblast growth factor receptor 4 (FGFR4) and phosphoinositide 3-kinase (PI3K)/mammalian target of rapamycin (mTOR) signaling was investigated in PDAC models. A combination of targeted drug treatments reduced viability, clonogenic survival, migration, and suppressed signaling pathways involved in translational control. This supported a functional interaction between FGFR4 and PI3K-mTOR co-activation in …

Ex Vivo Sensitivity Assays With Molecular Validation To Guide Preclinical Drug Selection For Schwann Cell Tumors, Ethan W. Hass

Graduate Studies Theses and Dissertations 2026

Schwann cells are the glial cell of the peripheral nervous system. They are dependent on receptor tyrosine kinase-mediated RAS signaling, cell matrix adhesion, and cell-cell contact to proliferate during nerve development and repair. These pathways become deregulated with loss of function of the merlin tumor suppressor encoded by NF2, leading to benign tumors called schwannomas. Patients with NF2-related schwannomatosis (NF2-SWN) develop bilateral vestibular schwannomas that cause hearing loss, tinnitus, and death from brainstem compression if untreated. Severe NF2-SWN causes multiple spinal and peripheral schwannomas and neuropathic pain. Schwannomas require careful monitoring, trial and error use of repurposed anti-cancer …

The Influence Of Limb Dominance On Neural Activation Patterns While Performing Different Motor Control Tasks, Kevin A. Hooks

Graduate Studies Theses and Dissertations 2026

Humans interact with our environment in many ways. One of the most outward-facing aspects of this is limb dominance. The neural underpinnings of limb dominance remain in question today. The objective of this research was to use different methods to analyze neural activation while subjects performed upper extremity motor tasks while recording electroencephalography (EEG). The first experiment used decoding methods to examine whether neural signals collected from the contralateral limb could be used to train a classifier to test on the ipsilateral limb during a reach-to-grasp or reach-to-touch task. It was determined above chance level that within-subject action and direction …

Investigating The Anti-Apoptotic Properties Of The N-Terminus Of The Chlamydia Trachomatis Effector Tarp, Kasey S. Jordan

Graduate Studies Theses and Dissertations 2026

As the most prevalent bacterial STI in the United States and leading cause of infection-related blindness worldwide, the obligate intracellular bacteria, Chlamydia trachomatis (Ct), poses a significant threat to public health. As an obligate intracellular bacteria, invasion of the host cell is a crucial step in Chlamydia’s developmental cycle. To drive invasion, Ct injects prepackaged effectors into the host cell using a T3SS. An early Chlamydial effector, translocated actin recruiting phosphoprotein, or Tarp, interacts with host cell actin to promote invasion, mediated via Tarp’s C-terminal region. Although the function of Tarp’s C-terminus is well defined, the Tarp’s N-terminus …

Homoplasy Of Maxillary Orthognathism In Derived Homo, Jared Jordan

Graduate Studies Theses and Dissertations 2026

The evolution of the orthognathic condition in the genus Homo represents a monumental transition toward the facial morphology of modern humans from that of earlier hominins, but the phylogenetic positioning of this transformation remains poorly understood. This study investigated temporal trends in maxillary and mandibular prognathism in the genus Homo, and whether maxillary and mandibular shortening in East Asian H. erectus and later East Asian Middle Pleistocene Homo (e.g., Homo longi) represent independent derivations of orthognathic morphology, thereby making orthognathism a homoplasy in the genus Homo. Using a sample of roughly 109 cranial and mandibular specimens spanning …

Advances In Active And Passive Integrated Photonic Devices On Thin-Film Lithium Niobate, Pooja S. Kulkarni

Graduate Studies Theses and Dissertations 2026

Thin-film lithium niobate (TFLN) has emerged as a powerful platform for integrated photonics, combining the exceptional electro-optic, nonlinear, and optical properties of bulk lithium niobate with the scalability and compactness of planar nanophotonic technologies. Building on these principles, advanced device architectures such as adiabatic dichroic filters have demonstrated exceptional spectral performance spanning over two octaves of bandwidth on the TFLN platform. Beyond reciprocal devices, it also offers promising pathways toward integrated nonreciprocal components. By leveraging broadband filtering structures and advanced nonlinear photonic design strategies, compact and monolithic optical isolators can be envisioned without relying on traditional magneto-optic materials. In the …

Establishment Of An In Vitro Dss-Induced Epithelial Injury Model To Investigate Il-6 Signaling And Microbial Metabolite Mediated Barrier Recovery, Srijal Kunwar

Graduate Studies Theses and Dissertations 2026

Dextran sodium sulphate (DSS) is widely used in vivo to induce epithelial injury; however, the molecular effects of DSS on intestinal epithelial cells, that investigate inflammatory and metabolic repair mechanisms remain unclear. Here, we establish an in vitro DSS-induced epithelial damage model using differentiated Caco-2 cell monolayer. Exposure to 2.5%(w/v) DSS for 24 hours resulted in significant upregulation of pore forming protein Claudin-2(2.677-fold) and oxidative marker, NOX-1(1.866-fold), accompanied by increased ROS production confirmed via DHE staining. Increased epithelial permeability was observed by TEER analysis, without significant cytotoxicity. Surprisingly, ELISA analysis revealed significant downregulation of IL-6 (p-value …

Multi-Scale Drivers Of Beetle Biodiversity In A Rapidly Urbanizing Region, Alessandra Pandolfi

Graduate Studies Theses and Dissertations 2026

As cities continue to expand, designing urban landscapes that balance ecological resilience with biodiversity conservation has emerged as a pressing challenge. This dissertation examines urban ecosystems as complex and dynamic environments shaping plant and animal communities, with a particular focus on ground-dwelling beetles as ecologically significant indicators of environmental change. Conducted across the Orlando metropolitan area, one of the fastest-growing regions in the United States, this dissertation integrates experimental and observational approaches. Specifically, it aims to (1) test sustainable landscaping practices, (2) compare the ecological outcomes of conventional and alternative residential landscape designs, and (3) assess how gradients of urbanization …

The Biosynthesis Of The Natural Product N-Nitroglycine, Gabriel Padilla

Graduate Studies Theses and Dissertations 2026

N-Nitroglycine (NNG) is a nitramine [R(R′)N–NO2; R′=H or alkyl] natural product (NP) produced by certain actinomycetes, such as Streptomyces noursei. At the beginning of this project, the biosynthesis of this NP was unknown. The goal was to determine how NNG and its nitramine moiety are enzymatically formed. This could lead to the discovery of novel nitramine NPs with diverse applications as well as alternative/green synthesis of nitramine explosives. This work begins by examining diverse biochemical N–N bond formation strategies. Nitrogen cycle enzymes, nitration of NPs, kutzneride biosynthesis, the aspartate/nitrosuccinate superfamily, hydrazinoacetic acid (HAA) biosynthesis, and streptozocin biosynthesis are among the …