Life Sciences Commons^™

Role Of Sox18 In Promoting Tumorigenesis In Pediatric Cancer Cell Lines, Cherubina S. Rubannelsonkumar

Honors Program Theses and Research Projects

Sarcomas constitute a high percentage (∼13%) of cancer-related deaths among pediatric patients between 0-19 years of age, with Rhabdomyosarcoma (RMS) being the most common pediatric soft tissue sarcoma and Ewing Sarcoma being the second most common malignant bone tumor in children. Yet, survival for those who develop such metastatic sarcomas remains below 20-30%. Interestingly, SOX family proteins are known to be up regulated in various cancer types and play a role in cancer progression (tumorigenesis, metastasis, etc.). More specifically, targeted knockdown of SOX18 has been shown to suppress various tumorigenic properties in cancer cell lines including osteosarcoma cells, hepatocellular carcinoma …

People That Score High On Psychopathic Traits Are Less Likely To Yawn Contagiously, Andrew Gallup, Mariska E. Kret, Omar T. Eldakar, Julia Folz, Jorg J. M. Massen

Biology Faculty Articles

Considerable variation exists in the contagiousness of yawning, and numerous studies have been conducted to investigate the proximate mechanisms involved in this response. Yet, findings within the psychological literature are mixed, with many studies conducted on relatively small and homogeneous samples. Here, we aimed to replicate and extend upon research suggesting a negative relationship between psychopathic traits and yawn contagion in community samples. In the largest study of contagious yawning to date (N = 458), which included both university students and community members from across 50 nationalities, participants completed an online study in which they self-reported on their yawn contagion …

Descriptive Study Of A Healthy Weight Management Program For Overweight Or Obese Children And Youth, Chad Roberts, Pam Kasyan-Howe, Kristin Domville, Lisa Schubert

Fall 2021 Virtual OTD Capstone Symposium

The rise in obesity among children and youth in the last 20 years is a public health concern. The prevalence of childhood obesity has multiplied worldwide in the last four decades (“The Heavy Burden of Obesity and the Economics of Prevention,” 2019). Obesity is a complex chronic disease in which abnormal or excessive accumulation of body fat impairs health (Wharton et al., 2020). Childhood obesity can profoundly affect children's physical health, social and emotional well-being, and self-esteem (Rankin et al., 2016 & Purnell et al., 2018). The problem is a lack of an occupation-based evaluation tool utilized to link occupational …

A Review Of Transformative Strategies For Climate Mitigation By Grasslands, Nuria Gomez-Casanovas, Elena Blanc-Betes, Caitlin E. Moore, Carl J. Bernacchi, Ilsa Kantola, Evan H. Delucia

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Grasslands can significantly contribute to climate mitigation. However, recent trends indicate that human activities have switched their net cooling effect to a warming effect due to management intensification and land conversion. This indicates an urgent need for strategies directed to mitigate climate warming while enhancing productivity and efficiency in the use of land and natural (nutrients, water) resources. Here, we examine the potential of four innovative strategies to slow climate change including: 1) Adaptive multi-paddock grazing that consists of mimicking how ancestral herds roamed the Earth; 2) Agrivoltaics that consists of simultaneously producing food and energy from solar panels on …

An Investigation Of Chronic Pain As A Much-Neglected Symptom Of Hypermobile Ehlers-Danlos Syndrome, Sydney Collins

Honors Theses

Ehlers Danlos Syndrome is a group of heritable loose connective tissue disorders with 13 distinguished subtypes. The hypermobile type of Ehlers Danlos Syndrome (hEDS) is the most common subtype and is caused by a genetic mutation that leads to defective collagen fibrils. This leads to joint instability and hypermobility, skin elasticity, widespread pain, fatigue, and generalized tissue fragility. Chronic pain is reported to be a symptom in as high as 92% of the hEDS population (Voermans et al., 2010); despite this prevalence, there is a significant lack of research, awareness, and treatment standardization regarding pain in hEDS. This literature review …

Hydrodynamic Assessment Of Natural And Nature-Based Features For Escatawpa River And Grand Bay In The Northern Gulf Of Mexico, Peter Bacopoulos, Karim Alizad, Davina Passeri, Matthew Bilskie, Stephen Medeiros, Scott Hagen

Faculty Publications

This presentation showcases a hydrodynamic assessment of natural and nature-based features (NNBFs) for the Pascagoula River, the Escatawpa River and Grand Bay, located along the Mississippi coast of the northern Gulf of Mexico. Two separate NNBF projects are being considered to: (1) restore the historical footprint (ca. 1848) of Grand Batture Island for coastal protection purposes; and (2) reconnect the hydraulics between the Escatawpa River and Grand Bay for ecosystem services purposes. The intended coastal protection benefits of the first project include buffering agency to wave attack and attenuation of storm surge with the restored island. The intended ecosystem services …

Predation Efficiency Of Lionfish (Pterois Volitans And P. Miles) In Differing Levels Of Habitat Complexity, Abigail Ehlers Kimbrel

All HCAS Student Capstones, Theses, and Dissertations

The invasion of the Indo-Pacific lionfish (Pterois sp.) into the Atlantic and Caribbean has received attention around the globe. Venomous spines, over-sized fanlike pectoral fins, slow movement, and novel feeding strategies have allowed lionfish to become effective apex- predators. Recent research has determined that lionfish use inshore mangrove habitats as foraging grounds, which are also used as nursery habitats for juvenile fish, thus reducing recruitment of native fish populations. The purpose of this study was three-fold: (1) determine the predation efficiency of lionfish in differing mangrove habitat complexities, (2) examine the influence of temperature and behavior of prey commonly found …

Optimizing The Time To Transfer Sexually Produced Corals Of Porites Astreoides, Agaricia Agaricites, And Montastraea Cavernosa To An Offshore Nursery, Rachel Ionata

All HCAS Student Capstones, Theses, and Dissertations

As reefs continue to decline globally and become unable to recover on their own, restoration becomes essential to abate reef degradation and boost reef recovery until the main sources of the degradation are addressed. Sexual propagation is an important restoration technique that still requires optimization. One of the major knowledge gaps is determining the optimal time to transfer newly-settled sexually-produced corals from an aquarium to an offshore nursery without compromising their survival and growth. This study transferred settlers from Porites astreoides, Agaricia agaricites, and Montastraea cavernosa to an offshore nursery at approximately one week, five weeks, and nine weeks post-settlement, …

A Review Of Calcineurin Biophysics With Implications For Cardiac Physiology, Ryan B. Williams

Theses and Dissertations

Calmodulin is a prevalent calcium sensing protein found in all cells. Three genes exist for calmodulin and all three of these genes encode for the exact same protein sequence. Recently mutations in the amino acid sequence of calmodulin have been identified in living human patients. Thus far, patients harboring these mutations in the calmodulin sequence have only displayed an altered cardiac related phenotype. Calcineurin is involved in many key physiological processes and its activity is regulated by calcium and calmodulin. In order to assess whether or not calcineurin contributes to calmodulinopathy (a pathological state arising from dysfunctional calmodulin), a comprehensive …

Examining The Need For Parental Satisfaction With The Msu Extension Equine Assisted Therapy Program, Ashlyn Ann Kiker

Theses and Dissertations

Animal assisted interventions are becoming more prevalent in our society. Animal interventions have become a popular therapy technique that exist to aid a variety of disabilities. Equine therapeutic riding has become one of the most popular forms of animal assisted therapy for both children and adults. While research shows the importance and benefits of animal assisted therapy for children, there is a lack of research concerning parental satisfaction within therapeutic riding programs.

This study tested a newly developed instrument for measuring parental satisfaction for therapeutic riding programs. The Parent/Caregiver Evaluation Tool (Kerr, 2021) was administered to parents/caregivers whose children participated …

Positive Correlation Between A3 Subunit Of Glycinin And Firmness Of Tofu Made From Soybeans Grown In Three Locations Over Two Years, Ruiqi Chen

Theses and Dissertations

Producing desirable firmness is important in manufacturing tofu from soybeans. This study’s objective was to explore the environmental impact (location and year) on soybean chemical components and identify the correlations between chemical composition and the firmness of tofu made from soybeans planted in three locations over two years. Seventeen soybean Plant Introductions (PI) from the USDA Soybean Germplasm Collection and eight check varieties were planted in Mississippi, Virginia and Missouri in 2017 and 2018. Protein subunit composition, protein secondary structure, phytic acid content, Ca²⁺ and Mg²⁺ content were determined. The result showed that A₃ subunit content was …

Initial Characterization Of Prna From Burkholderia Ambifaria: Developing An Nadph-Dependent Activity Assay For Tryptophan Halogenation, Mahmuda Akter

Theses and Dissertations

Some bacteria produce a potent antifungal agent (pyrrolnitrin) from tryptophan using four dioxygen dependent steps to outcompete other microbes. Each step of this process is catalyzed by an oxygenase encoded by the prnABCD cassette. The first enzymatic step in pyrrolnitrin biosynthesis is the regioselective chlorination of tryptophan to form 7-chlorotryptophan. This halogenation is catalyzed by PrnA, a Flavin dependent oxygenase, which has been isolated and characterized from P. fluorescens. The pyrrolnitrin biosynthesis pathway (prnABCD) has been also observed in the Burkholderia genus. This thesis comprises my studies on the expression, purification, and characterization of PrnA from Burkholderia ambifaria. Beyond the …

Genetic Characterization Of Antimicrobial Activities Of Endophytic Bacteria Burkholderia Strains Ms455 And Ms389, Jiayuan Jia

Theses and Dissertations

Strains MS455 and MS389, endophytic bacteria, were isolated from healthy soybean plant growing adjacent to a patch of plants affected by charcoal rot disease, caused by the fungal pathogen Macrophomina phaseolina. The complete genomes of both strains were sequenced and identified as Burkholderia species Strain MS455 exhibits broad-spectrum antifungal activities against economically important pathogens, including Aspergillus flavus. Random and site-specific mutations were employed in discovery of the genes that share high homology to the ocf gene cluster of Burkholderia contaminans strain MS14, which is responsible for production of the antifungal compound occidiofungin. RNA-seq analysis demonstrated ORF1, a …

Dissection Of The Genetic Architecture Of Grain Quality In Rice, Shuai Liu

Theses and Dissertations

Rice is an important human staple food for over half of the world’s population. Amylose content (AC), gelatinization temperature (GT), grain protein content (GPC), percentage grain chalkiness (PGC), and mineral content are important parameters for evaluating rice quality, which attracts customers and breeders. Only limited genes or QTLs (OsAAP6, OsGluA2, OsASN1, Chalk5, OsHMA3, etc.) are reported regulating rice GPC, PGC, and mineral content due to the lack of genetic knowledge and molecular markers. To dissect the genetic architecture of rice grain quality regulation, genome wide association studies (GWAS) were performed using two populations …

Virtual Reality And Postural Control: The Virtual Moving Room Paradigm, Hannah R. Freeman

Theses and Dissertations

Virtual reality (VR) is becoming increasingly important in balance training. However, the influence of VR generated visual perturbation, through the novel virtual moving room paradigm, is unknown. Thirty healthy individuals had their static balance assessed on a BTrackS balance plate under eight different conditions: baseline eyes open and eyes closed with and without VR, unexpected toward and away moving VR perturbation, and expected toward and away moving VR perturbation. Multiple statistical analyses were conducted, and the results revealed significantly higher postural sway variables in the unexpected moving toward trials compared to the other moving room conditions; significantly higher postural sway …

Efficiency Of Weights Matrix Specification In The Spatial Error Model, Cannon Kent

Theses and Dissertations

This study investigates and quantifies the effect of different specifications of the spatial weights matrix (��) on estimates and inferences in the context of a regression model using the lattice perspective with polygon-type data. The study also investigates an alternative to the specification of �� by estimating a spatial variance-covariance matrix based on known features of the spatial data. Previous literature has addressed the a priori construction of �� and selection criteria but assumes point-type data. This study’s primary contribution is the setup of a true and known benchmark that allows the comparison of the different specifications of ��. This …

Effects Of The Inclusion Of Rice Hull Derived Bio-Oil On Wood Pellet Production, Tyler E. Lowe

Theses and Dissertations

Wood pellet production has become an advancing industry for the sake of reducing greenhouse emissions into the atmosphere especially, in European Union countries. Researchers and industry executives seek new methods and materials to improve the pelletization process. Rice hulls or husks has the potential to aid in wood pelletization as they possess high calorific values. This study focuses on using rice hull derived bio-oil from pyrolysis, which will also decrease ash content, as an additive to aid in the wood pelletization process. Using two groups of rice hull derived bio-oil as an additive in wood pelletization: Group 1 uses heavy …

Modulation Of Effector Gene Expression In The Blood-Feeding Arthropods Cimex Lectularius Linnaeus, (Hemiptera: Cimicidae) And Aedes Albopictus Skuse, (Diptera: Culicidae), Travis C. Van Warmerdam

Theses and Dissertations

Hematophagy, or blood-feeding behavior, has independently evolved multiple times in the class Insecta and results in the transmission of vector-borne parasites, bacteria and viruses causing 17% of all infectious diseases and over 700,000 human deaths annually. One of the major challenges in studying the discrete mechanisms involved in host-response to hematophagy or the host seeking behavior of hematophagous insects, is the vast amount of signaling processes and genes that are inextricably tied to these interactions. These experiments used the clustered regularly interspersed short palindromic repeat (CRISPR)/CRISPR-associated (Cas) and dsRNA knock-down technologies to examine mechanisms of immunological reactivity and host-seeking behavior. …

Implementing Food Science-Based Instruction In Career Technical Education Courses, Jasmine D. Hendrix

Theses and Dissertations

Students are exposed to food concepts in agriculture-based career technical education (CTE) courses which provide a gateway for students to become more aware of food science career pathways. Professional development for Mississippi (MS) CTE teachers is needed to effectively implement food science-based instruction since there is not a food science curriculum framework adopted in MS. The objective of this study was to assess a food science professional development training for MS CTE teachers that would increase their self-perceived knowledge of food science, self-perceived ability to conduct food science skills, and their self-efficacy to implement food science-based instruction. Thirty-one teachers participated …

Seasonal Management Strategies For Tarnished Plant Bug, Lygus Lineolaris (Palisot De Beauvois), In Midsouth Cotton Production Systems, Ryan Taylor Mann

Theses and Dissertations

The tarnished plant bug, Lygus lineolaris (Palisot de Beauvois), is considered the most economically important pest of midsouth cotton. This study was designed to understand the impact of tarnished plant bug populations in the later weeks of flowering cotton and residual effects of novaluron with subsequent applications. Experiments evaluated dynamic threshold approaches in the later flowering period of midsouth cotton, the impacts of novaluron plus subsequent applications at the 3^rd week of square, as well as residual properties in a laboratory experiment. Results suggest that a dynamic late season approach can reduce the amount of insecticide applications targeting tarnished …

Molecular, Somatic, And Performance Characteristics Of Broilers Exhibiting Woody Breast Myopathy, And The Effects Of Dietary And Challenge Intervention Strategies, Linan Jia

Theses and Dissertations

Woody breast (WB) is a meat quality problem that has caused significant economic losses for the poultry industry. Ross × Ross 708 chicks were randomly assigned to a 3 (diet) × 2 (cocci challenge) × 2 (sex) factorial arrangement of treatments. The three diets included the control diet (corn-soybean meal basal diet), antibiotic diet (basal diet + 6.075 mg bacitracin /kg feed), and probiotic diet (basal diet + 2.2 × 108 CFU Bacillus subtilis PB6 /kg feed). Birds in the cocci challenge treatment group received 20 × the live cocci vaccine as an inoculum on d 14. Growth performance and …

Minimizing Off-Target Herbicide Movement Using Novel Application Technology, Hayden B. Quick

Theses and Dissertations

Drift is a point of contention with pesticide applications, causing the need to research application methods that provide consistent efficacy while minimizing off-target movement. Experiments were conducted to evaluate eight undiluted herbicides on invasive woody plants, Chinese tallow (Triadica sebifera) and Callery pear (Pyrus calleryana), when applied individual plant treatment (IPT) via hack-and-squirt. Applications of undiluted aminocyclopyrachlor or imazapyr at 1 ml per 7.6 cm of tree diameter at breast height (DBH) made in the spring provided superior control over other herbicides or application timings. CamelBak® hydration reservoirs were evaluated for storage durability with eight undiluted herbicides. A third study …

Physiological Roles Of Selenoprotein H In Mice, Qingzhou Wang

Theses and Dissertations

Low-hierarchy selenoproteins are sensitive to selenium (Se) deficiency and are proposed to confer the protection of body Se against age- and development-related diseases. Selenoprotein H (SELENOH), a low-hierarchy selenoprotein, is greatly downregulated by both dietary Se deficiency and age. To explore physiological roles of SELENOH, Selenoh knockout mice were employed.

Segregation analyses demonstrated reduced frequencies of homozygotes and heterozygotes among the neonates of the breeding combinations of Selenoh^+/- males vs. Selenoh^+/- or Selenoh^-/- females, demonstrating essential roles of SELENOH in embryogenesis. Litter sizes from these two breeding groups were comparable with control, suggesting a role of SELENOH …

Field Durability Test Of Clt Wall Envelope Using Physical Barriers Against Termites And Structural Performance Of Nailed Hold-Down Brackets Connected To Fungus-Exposed Clt Walls, Kamal Neupane

Theses and Dissertations

The effectiveness of using commercial polyethylene flashing and stainless-steel mesh in CLT wall systems as the termite barriers were evaluated in a short-term field test. American Wood Protection Association (AWPA) E21's visual ratings ranged from 10 to 9 in the specimens showing little damage when no physical barriers were used. Termites were able to crawl beyond the physical barriers in few specimens showing the necessity of further research on height and installation method of physical barriers. On the second part, the effect of decay caused due to Postia placenta, a brown-rot fungus, on the structural performance of hold-down brackets connected …

Evaluation Of The Variable Rate Capabilities Of A Sprayer Equipped With Pulse Width Modulation Nozzle Control And Direct Chemical Injection Systems, William Walker

Theses and Dissertations

Variable-rate technologies coupled with broadcast spray systems serve to reduce chemical inputs, misapplication of chemicals, and environmental pollution, thus improving profitability and sustainability. Sprayer variable rate control involves using pulse width modulation (PWM) solenoids and/or direct chemical injection to adjust the application rate. The objectives of this research were to: outfit a conventional broadcast sprayer with PWM and direct inject technologies; evaluate the accuracy of the PWM system to control application rate for strait line and turn segments; and characterize the direct injection system’s transport delay time. For the PWM evaluation, the mean flow rate and coefficient of variation of …

Assessing The Potential For Lightning-Induced Damage In Commercial Broiler Houses In Mississippi And Alabama, Matthew R. Rowland

Theses and Dissertations

Modern broiler houses are expensive to build, maintain, and insure. Protecting them from extreme weather events is a major concern to producers, integrators, and insurance companies. Lightning strikes can cause catastrophic fires and electrical damage and can lead to costly bird losses. A greater understanding of the impact of lightning on the commercial broiler industries of MS and AL is needed. The objectives of this research were collection of baseline resistance data for broiler houses and equipment in both states, and mapping lightning strike densities across MS and AL, evaluating annual, monthly, seasonal, and diurnal patterns. 63.5% of surveyed broiler …

Relationship Of Winter Hair Coat Shedding Ability Of Purebred Angus Dams With Mineral Status, Apparent Forage Digestibility, Uterine Artery Hemodynamics, And Calf Growth And Development, Randy Hunter Burnett

Theses and Dissertations

Winter hair coat shedding ability in southern U.S. cattle production has previously been associated with differences in adjusted birth and weaning weights of calves from dams that shed by June or later (Gray et al., 2011; Cauble et al., 2019). This study was developed to research possible avenues of why these relationships exist with winter hair coat shedding ability. The primary objectives of the following experiments were to: 1) evaluate how mineral status relates to winter hair coat shedding ability, 2) evaluate how winter hair coat shedding ability relates to apparent digestibility of forage, 3) evaluate winter hair coat shedding …

Evaluating The Effects Of Pine And Miscanthus Biochar On Water Activity And Escherichia Coli Populations In Commercial Broiler Litter, Christopher Austin Marty

Theses and Dissertations

The decrease in subtherapeutic antibiotic administration in poultry has increased the need to address production challenges caused by pathogens, such as E. coli. One potential way to improve bird health and reduce bacterial infection is through the addition of litter amendments that absorb moisture. Biochar (BC) has previously been shown to increase water holding capacity in poultry litter, but its effects on E. coli mitigation are unknown. The objectives of this research were to 1) evaluate water activity of poultry litter amended with pine and miscanthus BC, and 2) determine the effects of different BC inclusion rates on litter …

Systematics And Population Structure Of Amblyomma Maculatum Group Ticks And Rickettsia Parkeri, An Emerging Human Pathogen In Southern Arizona, Usa, Michelle E. J. Allerdice

Theses and Dissertations

The recent discovery of Amblyomma maculatum sensu lato (s. l.) ticks in southern Arizona has renewed discussions around species designations for members of the Amblyomma maculatum tick group. Amblyomma maculatum s. l. from Arizona appear to be morphologically intermediate between A. maculatum sensu stricto (s. s.) and A. triste s. s. At present there is no conclusive species designation for the ticks from Arizona. My research focused on analyzing the systematics of both A. maculatum s. l. and Rickettsia parkeri, a common bacterial pathogen transmitted by these ticks.

In the laboratory, A. maculatum s. l. from Arizona and A. maculatum …

Evaluation Of Alternate Wetting And Drying Irrigation Management In Rice, Richard Lee Atwill Ii

Theses and Dissertations

Water level declines in the Mississippi River Valley Alluvial Aquifer (MRVAA) are attributed largely to withdrawals for rice (Oryza sativa L.) irrigation. This study was performed to determine if alternative irrigation strategies for rice could reduce withdrawal from the MRVAA without having an adverse effect on grain yield, grain quality, control of barnyardgrass, and profitability. Research was conducted in Stoneville, MS and 19 on-farm locations across the Delta region of Mississippi from 2014 through 2017 to determine the irrigation threshold for alternate wetting and drying (AWD) rice irrigation, the effect of AWD management on barnyardgrass control, and effects of …