Life Sciences Commons^™

Seasonal Changes In Mitochondrial Bioenergetics And Physiological Performance Of The Bluegill Sunfish, Lepomis Macrochirus, From A Shallow, Midwest River., Derick I. Lamptey, Ryan W. Sparks, Rolando Monte De Oca, Robert Skolik, Michael Menze, Eloy Martinez

Faculty and Staff Scholarship

As global temperature shifts due to anthropogenic impacts, the frequency and magnitude of extreme temperatures in shallow aquatic ecosystems are expected to increase. Previous studies performed on fish under fluctuating temperature regimes have reported increased physiological stresses, compared to stable thermal conditions, leading to morphological, behavioral and biochemical adaptations. Moreover, many studies have reported the effects of warmer temperatures on numerous aquatic species, but seasonal temperature changes experienced in aquatic systems are understudied. We surveyed changes in the bioenergetic machinery of a native fish population ( Lepomis macrochirus) in the temperate zone of North America and observed season-dependent thresholds in …

Kentucky Alfalfa Conference: A Team Approach To Alfalfa Education And Technology Transfer, Garry D. Lacefield, Monroe Rasnake, Christi L. Forsythe, Jimmy C. Henning, Bill Talley

IGC Proceedings (1977-2023)

Alfalfa has played an important role in providing farmers of Kentucky with a high yielding, high quality, persistent forage legume for many years. In 1980, a group of individuals under the leadership of University of Kentucky Forage Extension Specialists organized and implemented the first statewide educational event on alfalfa. This initial meeting gave rise to the Kentucky Alfalfa Conference that celebrated it’s twentieth anniversary in February 2000. This annual event has been co-sponsored by the University of Kentucky, Kentucky Forage and Grassland Council, along with other state and national groups including Certified Alfalfa Seed Council, Kentucky Department of Agriculture, Kentucky …

Developing A National Alfalfa Information System, David B. Hannaway, K. J. Hannaway, P. Sohn, S. Griffith, B. E. Avery, E. Nowick, W. F. Wedin, L. R. Vough, S. C. Bosworth, Garry D. Lacefield, Gary E. Bates, D. Undersander, N. P. Martin, J. Caddel, G. L. Kilgore, S. B. Orloff, A. M. Gray, R. Ditterline, T. Griggs, D. H. Putnam, M. J. Ottman

IGC Proceedings (1977-2023)

Using state-of-the-art telecommunication technologies, this project is developing a comprehensive knowledge resource for alfalfa (Medicago sativa L.); the National Alfalfa Information System (NAIS). This project will serve as an improved model for Extension educational programs. Alfalfa is the most important forage crop in the USA and grown worldwide for feeding millions of livestock and in many cropping systems. As a legume, it is important in sustaining the environment and the productivity of agriculture. Information needs are present in every state and internationally. The NAIS is being developed through national and international cooperation, putting the best science-based alfalfa information and …

Comparison Of Percutaneous Vs Oral Infection Of Hamsters With The Hookworm Ancylostoma Ceylanicum: Parasite Development, Pathology And Primary Immune Response, Richard D. Bungiro, Lisa M. Harrison, Blaise Dondji, Michael Cappello

Biology Faculty Scholarship

Background

Hundreds of millions of people in poor countries continue to suffer from disease caused by bloodfeeding hookworms. While mice and rats are not reliably permissive hosts for any human hookworm species, adult Golden Syrian hamsters are fully permissive for the human and animal pathogen Ancylostoma ceylanicum. Similar to humans, hamsters may be infected with A. ceylanicum third-stage larvae orally or percutaneously. Oral infection typically leads to consistent worm yields in hamsters but may not accurately reflect the clinical and immunological manifestations of human infection resulting from skin penetration.

Methodology/Principal findings

In this study we compared host responses …

Plasma Induced Reactive Oxygen Species-Dependent Cytotoxicity In Glioblastoma 3d Tumourspheres, Janith Wanigasekara, Carlos Barcia, Patrick J. Cullen, Brijesh Tiwari, James F. Curtin

Articles

The aim of this study was to determine the effects of a pin‐to‐plate cold atmospheric plasma (CAP) on U‐251 MG three‐dimensional (3D) glioblastoma spheroids under different conditions. 3D tumorspheres showed higher resistance to the CAP treatment compared to 2D monolayer cells. A single CAP treatment was able to induce cytotoxicity, while multiple CAP treatments augmented this effect. CAP was also able to induce cytotoxicity throughout the tumoursphere, and we identified that reactive oxygen species(ROS) plays a major role, while H2O2plays a partial role in CAP‐induced cytotoxicity in tumour-spheres. We conclude that ROS‐dependent cytotoxicity is induced uniformly throughout glioblastoma and epidermoid …

Insights Into Nebraska Rural Grocery Store Ownership, Marilyn R. Schlake, Shawn Kaskie, Greg Mckee, Charlotte Narjes, Jason Tuller, John Westra

Cornhusker Economics

In Fall 2020, University of Nebraska-Lincoln researchers started a research project to better understand the perceptions and experiences of transitioning a rural grocery store to new ownership. The project is part of the Heartland Challenge program funded by the Ewing Marion Kauffman Foundation. Researchers conducted a survey of Nebraska rural grocers to seek their input and determine future research directions. Sixty-seven of the 216 distributed surveys were completed either electronically or by print. Survey questions pertained to challenges and opportunities owners experienced while operating a rural grocery operation, community support and involvement in store transition, and motivations for continued operation …

Chronic Background Radiation Correlates With Sperm Swimming Endurance In Bank Voles From Chernobyl, Kati Kivisaari, Sara Calhim, Zbyszek Boratyríski, Timothy Mousseau, Anders P. Møller, Tapio Mappes

Faculty Publications

Sperm quantity and quality are key features explaining intra- and interspecific variation in male reproductive success. Spermatogenesis is sensitive to ionizing radiation and laboratory studies investigating acute effects of ionizing radiation have indeed found negative effects of radiation on sperm quantity and quality. In nature, levels of natural background radiation vary dramatically, and chronic effects of low-level background radiation exposure on spermatogenesis are poorly understood. The Chernobyl region offers a unique research opportunity for investigating effects of chronic low-level ionizing radiation on reproductive properties of wild organisms. We captured male bank voles (Myodes glareolus) from 24 locations in the Chernobyl …

Upregulation Of Cd36, A Fatty Acid Translocase, Promotes Colorectal Cancer Metastasis By Increasing Mmp28 And Decreasing E-Cadherin Expression, James Drury, Piotr G. Rychahou, Courtney O. Kelson, Mariah E. Geisen, Yuanyuan Wu, Daheng He, Chi Wang, Eun Y. Lee, B. Mark Evers, Yekaterina Y. Zaytseva

Surgery Faculty Publications

Altered fatty acid metabolism continues to be an attractive target for therapeutic intervention in cancer. We previously found that colorectal cancer (CRC) cells with a higher metastatic potential express a higher level of fatty acid translocase (CD36). However, the role of CD36 in CRC metastasis has not been studied. Here, we demonstrate that high expression of CD36 promotes invasion of CRC cells. Consistently, CD36 promoted lung metastasis in the tail vein model and GI metastasis in the cecum injection model. RNA-Seq analysis of CRC cells with altered expression of CD36 revealed an association between high expression of CD36 and upregulation …

Thrombospondin-1 Expression And Modulation Of Wnt And Hippo Signaling Pathways During The Early Phase Of Trypanosoma Cruzi Infection Of Heart Endothelial Cells, Ashutosh Arun, Kayla J. Rayford, Ayorinde Cooley, Tanu Rana, Girish Rachakonda, Fernando Villalta, Siddharth Pratap, Maria F. Lima, Nader Sheibani, Pius N. Nde

Publications and Research

The protozoan parasite, Trypanosoma cruzi, causes severe morbidity and mortality in afflicted individuals. Approximately 30% of T. cruzi infected individuals present with cardiac pathology. The invasive forms of the parasite are carried in the vascular system to infect other cells of the body. During transportation, the molecular mechanisms by which the parasite signals and interact with host endothelial cells (EC) especially heart endothelium is currently unknown. The parasite increases host thrombospondin-1 (TSP1) expression and activates the Wnt/β-catenin and hippo signaling pathways during the early phase of infection. The links between TSP1 and activation of the signaling pathways and …

Ranging Patterns And Site Fidelity Of Snubfin Dolphins In Yawuru Nagulagun/Roebuck Bay, Western Australia, Alexandra D’Cruz, Chandra Salgado Kent, Kelly Waples, Alexander M. Brown, Sarah A. Marley, Deborah Thiele, Holly C. Raudino, Yawuru Native Title Prescribed Body Corporate, Broome, Wa, Australia

Research outputs 2022 to 2026

For long-lived species such as marine mammals, having sufficient data on ranging patterns and space use in a timescale suitable for population management and conservation can be difficult. Yawuru Nagulagun/Roebuck Bay in the northwest of Western Australia supports one of the largest known populations of Australian snubfin dolphins (Orcaella heinsohni)—a species with a limited distribution, vulnerable conservation status, and high cultural value. Understanding the species’ use of this area will inform management for the long-term conservation of this species. We combined 11 years of data collected from a variety of sources between 2007 and 2020 to assess the ranging patterns …

Map Based Www Tool For Recommending Forage Species, M. H. Hall, D. A. Miller, J. H. Cherney

IGC Proceedings (1977-2023)

Selecting the “best” forage species or species mixture requires a knowledge and understanding of the consuming animal, soil characteristics, and forage species. The multivariate nature of the problem presents challenges to those interested in solving the practical problem of maximizing available resources. Earlier work at Cornell University resulted in a WWW-based interface that allowed the user to provide a soil type or zip code and additional management factors to initiate the species selection. This approach relies on either user knowledge of soil series or an implied relationship between zip code and soil series that may often be wrong. To improve …

Remote Sensing Application To Grassland Monitoring, Z. B. Nan

IGC Proceedings (1977-2023)

Application of remote sensing to the management of grassland resources, the role this plays in developing sustainable grassland farming systems and opportunities for further development are outlined. Use of remote sensing technologies in grassland monitoring has a history of more than 30 years. Both fine- and coarse-grained remote sensing techniques are used to monitor and study grasslands. Fine-grained techniques are used to study landscape scale processes through the use of sensors providing spatial resolution of a few meters, whereas coarse-grained techniques are used to study catchment scale areas, and even entire biomes, using satellite-based sensors with a spatial resolution of …

Modelling Grassland Ecosystems, J. H. M. Thornley

IGC Proceedings (1977-2023)

In this contribution a view of the promise and difficulties of modelling grassland is given. This is largely centred around work with a grassland ecosystem simulator known as the Hurley Pasture Model.

A brief introduction sets forth possible reasons for building a large ecosystem model, and stresses the importance of modelling objectives. It is suggested that a model is de rigeur for any research programme which aims to take a firm grasp of the complex responses of grassland. Mechanistic models are required to provide the understanding needed for intelligent and flexible management of grassland, whatever the prevailing environmental or economic …

Estimating Herbage Mass In Stargrass (Cynodon Nlenfuensis Var Nlenfuensis) Using Sward Surface Height And The Rising Plate Meter, D. E. Oliveira, S. R. Medeiros, L. J. M. Aroeira, L. G. Barioni, D. P. D. Lanna

IGC Proceedings (1977-2023)

Estimation of herbage mass is necessary both in research and farm management. Methods for such estimations should be inexpensive, rapid and reliable. Since estimations through hand clipping are time consuming, a range of indirect methods have been developed and tested in the literature. This study had the aim of comparing precision of two indirect methods, sward surface height (SSH) and the rising plate meter (RPM), to estimate herbage mass in stargrass pastures (Cynodon nlenfuensis var. nlenfuensis). Pre-grazing measurements of SSH and RPM readings were taken and compared with the paddock herbage mass. RPM readings were converted into centimeters. Average …

A Taste For Education And Research, Susan Vernon-Devlin, Robert Seltzer

Rosen Research Review

Pots, pans and commercial kitchen equipment are not the only things found in UCF Rosen College of Hospitality Management’s kitchen labs. A component for hospitality education and research, the college’s labs teach fourth year medical students to practice culinary medicine with a grain of salt, flip the kegs on undergraduates brewing beer, and serve as taste-and-test kitchens for restaurant brands looking to spice up a menu.

Impact Of Edds Dosage On Lead Phytoextraction In Contaminated Urban Residential Soils, Zhiming Zhang, Dibyendu Sarkar, Virinder Sidhu, Manas Warke, Rupali Datta

Michigan Tech Publications, Part 1

Lead (Pb) contamination in soils of residential properties due to peeling and chipping of Pb-based paint can cause human health problems. Phytoextraction is a green technology that has the potential to remediate soil Pb. The efficiency of phytoextraction is dependent on the geochemical forms of Pb in soil. A biodegradable chelating agent, ethylenediaminedisuccinic acid (EDDS), was previously shown to enhance Pb removal by facilitating phytoextraction. In this study, EDDS was tested at various concentrations for its potential in mobilizing Pb in urban residential soils in Jersey City, New Jersey, and San Antonio, Texas. Results show that the concentrations of plant-available …

Role Of Three Bird Species In The Life Cycle Of Two Sarcocystis Spp. (Apicomplexa, Sarcocystidae) In The Czech Republic, Ondřej Máca, David González-Solís

Harold W. Manter Laboratory of Parasitology: Library Materials

Birds are one of the groups involved in the development of Sarcocystis Lankester (1882), serving either as intermediate or definitive hosts. The white-tailed sea eagle Haliaeetus albicilla (Linnaeus, 1758), red kite Milvus milvus (Linnaeus, 1758) (both Accipitriformes) and common starlings Sturnus vulgaris Linnaeus, 1758 (Passeriformes) were examined to elucidate their participation in the development of Sarcocystis, as well as to determine the specific identity of the parasites based on morphological and especially molecular analyses. In 2020–2021, one white-tailed eagle, one red kite and five common starlings were parasitologically examined for the presence of Sarcocystis using flotation centrifugation coprological method …

Sensei: How Many Samples To Tell A Change In Cell Type Abundance?, Shaoheng Liang, Jason Willis, Jinzhuang Dou, Vakul Mohanty, Yuefan Huang, Ken Chen

Faculty, Staff and Student Publications

Cellular heterogeneity underlies cancer evolution and metastasis. Advances in single-cell technologies such as single-cell RNA sequencing and mass cytometry have enabled interrogation of cell type-specific expression profiles and abundance across heterogeneous cancer samples obtained from clinical trials and preclinical studies. However, challenges remain in determining sample sizes needed for ascertaining changes in cell type abundances in a controlled study. To address this statistical challenge, we have developed a new approach, named Sensei, to determine the number of samples and the number of cells that are required to ascertain such changes between two groups of samples in single-cell studies. Sensei expands …

Invited Perspective - Nutritional Needs And Implications For Children In Subsistence Marketplaces, Nagendra Rangavajla

Subsistence Marketplaces

Today, while the number of stunted children is decreasing in all geographies, the progress is not consistent. Moreover, there is an increasing prevalence of overweight and obesity among children and adolescent. Globally, about half of all children under five do not receive essential nutrients, often unnoticed until too late. On the other end of the spectrum, the incidence of overweight and obesity in 5-19 year old has increased from 4% in 1975 to 18% in 2016 ¹. These trends reflect a ‘triple burden of malnutrition’, a burden that impacts the survival, growth, and development of children, and in turn, …

Data From: Glucose Tolerance Of Iguanas Is Affected By High Sugar Diets In The Lab And Supplemental Feeding By Ecotourists In The Wild, Susannah S. French

Browse all Datasets

Physiological responses to both natural and anthropogenic perturbations involve changes in glucose use and metabolism to enable animals to respond to challenges. The responses are at least partially mediated via the glucocorticoids, and the proper functioning of these systems are critical to animal health. Prolonged high-sugar diets in humans can compromise physiological functions and lead to a range of diseases including diabetes. Such outcomes are less common in natural, non-human systems, likely because of evolutionarily based balances between sugar intake and the ability to process it. Due to both landscape changes and human feeding of wildlife, the introduction of unnatural …

Management-Intensive Grazing Impacts On Total Escherichia Coli, E. Coli O157: H7, And Antibiotic Resistance Genes In A Riparian Stream, Laura M. Rubeck, James E. Wells, Kathy Hanford, Lisa M. Durso, Walter H. Schacht, Elaine D. Berry

Department of Agronomy and Horticulture: Faculty Publications

The impacts of management-intensive grazing (MIG) of cattle on concentrations of total Escherichia coli, total suspended solids (TSS), and nitrate-nitrite nitrogen (NO₃+NO₂-N), and occurrence of E. coli O157:H7 and selected antibiotic resistance genes (ARGs) in stream water and/or sediments were evaluated. Cattle were grazed for twoweek periods in May in each of three years. Overall, grazing increased total E. coli in downstream water by 0.89 log10 MPN/100 mL (p < 0.0001), and downstream total E. coli concentrations were higher than upstream over all sampling intervals. Downstream TSS levels also increased (p ≤ 0.0294) during grazing. In contrast, there was …

The Analysis Of Results From Paired Paddock Comparisons, David R. Kemp, P. M. Dowling, D. L. Michalk, G. Millar

IGC Proceedings (1977-2023)

Paired-paddock comparisons are a common way of evaluating new grassland practices at a scale more relevant to farmers. They are also being used to replace or augment designed experiments and can be the only source of information available on a practice. However, it is often uncertain if the differences between paddocks are significant. Importantly, a current trend among funding organisations is to support paddock comparisons. The need for valid procedures to compare unreplicated treatments is increasingly urgent. It is suggested that a range of tools be used to infer statistical significance from using typical error values from related studies or …

Length And Width To Estimate Dry Mass Of Panicum Maximum Cv. Tanzânia Leaves, L. G. Barioni, P. M. Santos, A. Coldebella, M. Corsi

IGC Proceedings (1977-2023)

An analysis of the relationship of leaf length (LL) and leaf width (LW) with leaf dry weight (LDW) in Panicum maximum was carried out with the objective of improving estimations of tissue flow in that plant. Data was collected in a mob grazing experiment with 28 days grazing interval sampled the day before grazing in 9 grazing cycles. Regression analysis revealed highly significant effect (P < 0.001) of both LL and LW on LDW. A lack of fit test gave strong evidence of non-linear relationship of LDW with LL (p < 0.05), fitting the model 1 0 LDW = β₀LL^β₁ , while LW presented a linear relation with LDW. LL was a better predictor of LDW than LW. LL solely or in combination with LW produced equations with …

Bioeconomic Model For Decision-Making On Fattening Beef-Cattle, G. Ferreira, O. Cardozo, J. M. Soares De Lima

IGC Proceedings (1977-2023)

Profitability and competitiveness of agricultural systems it is closely related with the technology used. Economic and financial appraisal of investments on pasture improvements, facilities, feed and management plans, etc. are often required in order to make decisions. A bio-economic simulation model was developed in order to support decision making on beef-cattle grazing fattening. The aim of the model is to show the relationship between the technological alternative analysed in physical terms as well as financial and economic. By this way it is possible to asset and relate the bio-economic impact of the different animal and pasture management alternative technologies. Results …

Sequential Injection Analysis: A Powerful Tool For Routine Soil And Plant Laboratories, A. R. A. Nogueira, F. V. Silva, M. Y. Kamogawa, G. B. Souza, L. F. M. Ferraz

IGC Proceedings (1977-2023)

Sequential injection analysis (SIA) present attractive characteristics for analyses in large scale. In SIA the analytical determination can be performed automatically reducing the number of steps usually involved in a chemical analysis. In order to demonstrate the advantages found in the implementation of a SIA procedure in a laboratory dedicated to routine analyze, the determination of volatile nitrogen in silage and soil samples has been performed. The nitrogen content was determined after NH₃ on-line separation in alkaline conditions by using a gaseous diffusion or a pervaporation unit for liquid-liquid separation. An ammonium tubular selective electrode detector was used for …

Detecting The Feeding Habitat Zone Of Albacore Tuna (Thunnus Alalunga) In The Southern Indian Ocean Using Multisatellite Remote Sensing Data, Sandipan Mondal, Yang-Chi Lan, Ming-An Lee, Yi-Chen Wang, Bambang Semedi, Wan-Ya Su

Journal of Marine Science and Technology–Taiwan

Remote sensing is an effective method for identifying potential feeding habitat zones. Spatial and temporal distribution patterns of albacore (ALB) tuna were studied using Taiwan longline fisheries data from 2009 to 2014. A generalized additive model (GAM) was used to compile a fishery database and statistically explore the relationship between distribution and environmental factors. Sea surface temperature (SST), sea surface chlorophyll-a concentration (SSC), sea surface salinity (SSS), sea surface height (SSH), mixed layer depth (MLD), and eddy kinetic energy (EKE) were examined. The results indicated SST as the largest contributor on ALB distribution, followed by SSS and SSC. Catches per …

Japanese Consumer Preference For Raw Fish: Best–Worst Scaling Method, Takahiro Sajiki, Yu-Heng Lu

Journal of Marine Science and Technology–Taiwan

Given the declining consumption of fish and shellfish in Japan, it is essential to better understand consumer needs to design policies that increase consumption. Therefore, we clarify the relative degree of importance Japanese consumers place on various attributes of raw fish during purchase based on a questionnaire survey employing best–worst scaling. We find that consumers prioritize freshness, price, taste, appearance, safety, seasonality, ease of cooking and eating, nutrition, origin, wild fish, and effect on natural environment and ecosystem, in that order. Wild fish has unexpectedly low importance, although Japanese consumers conventionally purchase captured fishery products rather than cultured ones. The …

Diel Vertical Distribution Patterns Of Pelagic Fish Larvae In Yilan Bay, Taiwan, Yi-Chen Wang, Su-Chen Tsai, Wen-Yu Chen

Journal of Marine Science and Technology–Taiwan

To study the diel migration distribution of larval fish in an area adjacent to the Kuroshio Current, samples were collected from six depths by using a Motoda horizontal net at a fixed station in Yilan Bay on July 30 and 31, 2007. A total of 591 larval fish were collected and assigned to at least 34 families and 57 species. The first five dominant species were Benthosama pterotum (40.95%), Diaphus B type (12.18%), Callionymidae spp. (8.46%), Gobiidae spp. (6.60%), and Apogonidae spp. (4.0%). The density of larval fish varied across time. The maximum larval density was 1220.34 individuals/1000 m3 at …

Long-Term Variation Of Sea Surface Temperature In Relation To Sea Level Pressure And Surface Wind Speed In Southern Indian Ocean, Sandipan Mondal, Ming-An Lee, Yi-Chen Wang, Bambang Semedi

Journal of Marine Science and Technology–Taiwan

Sea surface temperature (SST) is an essential parameter associated with fish habitat and changes in oceanic conditions. Long-term SST variation in relation to sea level pressure (SLP) and surface wind speed (SWS) was observed based on the National Oceanic and Atmospheric Administration (NOAA) data of southern Indian Ocean. Mean monthly time series showed that February and November were the warmest and coolest months, respectively. Spatial distribution showed that 0°S–30°S was warm with SST of 20°–30°C, which was higher than that at all other places throughout the year. SLP (> 1010 millibar) and SWS (> 12 m/s) were high at 20°S–40°S …

Economic Conditions For New Entrants In Coastal Fishing In Miyazaki Prefecture, Japan, Takahiro Sajiki, Yu-Heng Lu, Norie Nishiguchi, Sonomi Akamine, Masaharu Nishiguchi

Journal of Marine Science and Technology–Taiwan

A significant decrease in the number of fishery workers is be-coming a problem in Japan. For new entrants to become estab-lished in the region, they must be profitable as fishing busi-nesses. Using the case of Miyazaki prefecture in Japan, this paper identifies the economic conditions required to make longline fishing and bottom gillnet fishing profitable by ap-plying the net present value (NPV) method. When operating on 200 days, which is the guideline for new entrants in longline fishing, if the value of investment is less than 44.1% of the minimum required, the investment will not provide positive returns. However, the …