Life Sciences Commons^™

Navigating The “Covid Hangover” In Physiology Courses, Jennifer E. Schaefer

Biology Faculty Publications

Undergraduate educators and students must navigate lingering aftereffects of the COVID pandemic on education in the 2021–2022 academic year even as COVID continues to impact delivery of undergraduate science education. This article describes ongoing difficulties for undergraduate science, technology, engineering, and mathematics (STEM) students and educators and suggests strategies and easy-to-use resources that may help educators navigate the “COVID hangover” and ongoing COVID-related disruptions.

Relationships Between Reported Pandemic Impacts And Well-Being Of Rural Nebraskans, Rebecca J. Vogt

Cornhusker Economics

Since March of 2020, the coronavirus pandemic has impacted Nebraskans in many ways, including their physical health, mental health, work, and socialization. The 2021 Nebraska Rural Poll examined to what extent various elements of life were affected or disrupted by the pandemic as well as respondents’ general assessments of their well-being. Did rural Nebraskans’ assessments of the impacts the pandemic has had on their lives correlate with their assessment of their well-being?

Bovine Respiratory Microbiota Of Feedlot Cattle And Its Association With Disease, Jianmin Chai, Sarah F. Capik, Beth Kegley, John T. Richeson, Jeremy G. Powell, Jiangchao Zhao

Animal Science Faculty Publications and Presentations

Bovine respiratory disease (BRD), as one of the most common and costly diseases in the beef cattle industry, has significant adverse impacts on global food security and the economic stability of the industry. The bovine respiratory microbiome is strongly associated with health and disease and may provide insights for alternative therapy when treating BRD. The niche-specific microbiome communities that colonize the inter-surface of the upper and the lower respiratory tract consist of a dynamic and complex ecological system. The correlation between the disequilibrium in the respiratory ecosystem and BRD has become a hot research topic. Hence, we summarize the pathogenesis …

Invertebrate Grazing On Live Turtlegrass (Thalassia Testudinum): A Common Interaction That May Facilitate Fungal Growth, Anjali D. Boyd, Nia S. Walker, Stephanie R. Valdez, Y. Stacy Zhang, Andrew H. Altieri, Vladislav Gulis, Caitlin Crain, Brian Silliman

Biology

In coastal wetlands and tropical reefs, snails can regulate foundation species by feeding on marsh grasses and hard corals. In many cases, their impacts are amplified because they facilitate microbial infection in grazer-induced wounds. Whether snails commonly graze live plants and facilitate microbial growth on plants in tropical seagrass systems is less explored. On a Belizean Caye, we examined patterns in snail-generated grazer scars on the abundant turtlegrass (Thalassia testudinum). Our initial survey showed the occurrence of snail-induced scarring on live turtlegrass blades was common, with 57% of live leaves scarred. Feeding trials demonstrated that two of five common snails …

Creating An Interdisciplinary Curriculum Within The Undergraduate Arts And Sciences Through Agar Art, Julie Torruellas Garcia, Kandy Lopez, Véronique Côté, Katie E. Crump

Biology Faculty Articles

Evidence-based studies on the benefits of integrating STEM into the arts are limited; however, some suggest that it can lead to improved scientific literacy and new approaches for artistic scholarship. Unfortunately, undergraduate education often creates disciplinary silos where the two are not integrated. Here, we discuss a unique collaboration between professors in the art and biology departments. Our goal was to integrate science into art courses using an agar art activity. We hypothesized that art students could effectively learn microbiology laboratory techniques and use them as novel tools for artistic practice. The activity was integrated into two to four sessions …

Improved Chromosome-Level Genome Assembly Of The Glanville Fritillary Butterfly (Melitaea Cinxia) Integrating Pacific Biosciences Long Reads And A High-Density Linkage Map, Olli-Pekka Smolander, Daniel Blande, Virpi Ahola, Pasi Rastas, Jaakko Tanskanen, Juhana Kammonen, Vicencio Oostra, Lorenzo Pellegrini, Suvi Ikonen, Tad Dallas, Michelle F. Dileo, Anne Duplouy, Iihan Cem Duru, Pauliina Halimaa, Aapo Kahilainen, Suyog S. Kuwar, Sirpa O. Kärenlampi, Elvira Lafuente, Shiqi Luo, Jenny Makkonen, Abhilash Nair, Narua De La Paz Celorio-Mancera, Ville Pennanen, Annukka Ruokolainen, Tarja Sundell, Arja I. Tervahauta, Victoria Twort, Erik Van Bergen, Janina Österman-Udd, Lars Paulin, Mikko J. Frilander, Petri Auvinen, Marjo Saastamoinen

Faculty Publications

Background

The Glanville fritillary (Melitaea cinxia) butterfly is a model system for metapopulation dynamics research in fragmented landscapes. Here, we provide a chromosome-level assembly of the butterfly's genome produced from Pacific Biosciences sequencing of a pool of males, combined with a linkage map from population crosses.

Results

The final assembly size of 484 Mb is an increase of 94 Mb on the previously published genome. Estimation of the completeness of the genome with BUSCO indicates that the genome contains 92–94% of the BUSCO genes in complete and single copies. We predicted 14,810 genes using the MAKER pipeline and …

Evaluating Greenhouse Gas Emissions And Climate Mitigation Goals Of The Global Food And Beverage Sector, Megan Reavis, Jenny Ahlen, Joe Rudek, Kusum Naithani

Biological Sciences Faculty Publications and Presentations

The dramatic increase in greenhouse gas (GHG) emissions by humans over the past century and a half has created an urgency for monitoring, reporting, and verifying GHG emissions as a first step toward mitigating the effects of climate change. Fifteen percent of global GHG emissions come from agriculture, and companies in the food and beverage industry are starting to set climate goals. We examined the GHG emissions reporting practices and climate goals of the top 100 global food and beverage companies (as ranked by Food Engineering) and determined whether their goals are aligned with the science of keeping climate warming …

Grasslands Classification In Yobe State Nigeria Using Integrated Orderly Classification System Of Grassland (Iocsg), S. Usman, M. G. Bala, Y. Shen

IGC Proceedings (1977-2023)

Integrated Orderly Classification System of Grassland (IOCSG) was initiated by Professor Ji-Zhou Ren in the 1960s and it has satisfactorily served to direct theory and practice of grassland classification for the last several decades in China. In this study IOCSG is adopted to classify the grasslands in Yobe state Nigeria, using 30 years (1984-2013) data on precipitation and temperature from 12 satellite stations across the state. Humidity (moisture index) was estimated as the ratio of precipitation per annum to annual temperature. The results revealed a trend of decline in precipitation and moisture index with latitude; from 772.63 mm and 0.72 …

Forb Counts In Grassland Are Sensitive To Analytical Method, Gene A. Fults

IGC Proceedings (1977-2023)

An analysis of forbs from 9 years of Plant Census protocol (NRI 2019) is compared to data from the Line Point Intercept (LPI) protocol. Both protocols were conducted on the same geospatially separate macroplots (N=1230). The macroplots locations were stratified by NRI based on a land hierarchy which has had long-term use in the United States (Salley etal 2015). This ties vegetation and land together in the data. The analysis showed that the 15-minute plant census method increased forb species data by 29 percent over the LPI method which takes 40-60 minutes. Both methods captured similar total ratios and rank …

The Relationship Between Grassland Composition On Stemborer Abundance On Grasses Surrounding Maize Farms, Nelima Maryselah, Mutyambai M. Daniel, Kuyah Shem, Netondo Godfrey, Mattias Jonsons

IGC Proceedings (1977-2023)

Grasslands are known to host crop pests and may act as the reservoir of pests affecting maize production. This research aimed at determining the effect of grassland composition on abundance of stemborers on grasses sorrouding maize farms in western Kenya. The experimental set up followed a complete random block design (CRBD); with four elevations at Lambwe, Homabay, Luanda and Mt. Elgon assigned as blocks based on different altitudes. Sampling was done in two seasons, during the short rain season of 2019 and the long rain season of 2020. Grasslands surrounding four pairs of push-pull and non-push-pull maize farms were assessed. …

Describing Cattle Grazing Distribution In Communal Rangelands Of The Eastern Cape South Africa: Towards Improving Livestock Rangeland Use, B. Gusha, A. R. Palmer

IGC Proceedings (1977-2023)

This study used global positioning systems (GPS) to understand grazing distribution of three cattle in the communal rangeland of Eastern Cape, South Africa. Free ranging cattle were randomly selected from participating households and were fitted with neck GPS collars, which recorded geographic position every five-minute interval during wet and dry season. The study identified three production domains where cattle were reported to be spending most of their time grazing, separated by bound polygons on google earth Pro to further extract MODIS EVI???. The recorded GPS points were loaded into google earth Pro to analyse the distribution of cattle grazing in …

Degradation Issue Of Fescue-Forbs Rangeland In The Mountain Steppe Of Mongolia, Otgontuya Lkhagvajav, Lkhagvajav Namdag, Munkhbat Chimed

IGC Proceedings (1977-2023)

The total area of Fescue-forbs rangeland in Mongolia is 10 million hectares, accounting for 8.5% of total rangeland area. The Fescue-forbs rangeland is the main type of rangeland in the mountain steppe. The Mongolian rangeland has degraded in the last two decades mainly due to overgrazing and climate change. The main objective of this study was to determine the change of plant community and reduction of frequency and productivity of the dominant species Festuca lenensis in different degradation states of rangeland. The dominant species and main plant functional groups have completely changed in Fescue-forbs rangeland in all three states of …

Observations On Regeneration Of The Pedipalp And Legs Of Scorpions, Martin Watz, Jason A. Dunlop

Euscorpius

An Opisthacanthus asper (Peters, 1861) (Hormuridae) shows a relatively rare example of pedipalp regeneration in which the lost tibia and tarsus was replaced by a smaller, curved element of uncertain homology to either the fixed or free finger. A comparable abnormal palp described in the literature hints that pedipalps can only regenerate a structure of this form, regardless of the site of amputation. An Olivierus caucasicus (Nordmann, 1840) (Buthidae) is described in which claws (pretarsus) of leg III regenerated directly at the distal end of the tibia, while in leg IV the claws regenerated at the end of a truncated …

Breadth Of Vaccinated Cancer Patient Humoral Response To Sars-Cov-2 Spike Protein And Rbd Variants, Julia Corman, Joey Breckenridge Jr., Adrienne M. Bushau-Sprinkle, Krystal T. Hamorsky, Joshua L. Fuqua

Posters-at-the-Capitol

SARS-CoV-2, the virus responsible for the COVID-19 of which several variants have emerged, such as the B.1.351 SARS-CoV-2 variant. The Receptor Binding Domain (RBD), located within the Spike protein is an immunogenic epitope for potent neutralizing antibodies. Current mRNA vaccines encode for the Spike protein, allowing the body to build antigen-specific antibodies. Assays measuring protective antibodies are essential to manage the COVID-19 pandemic and can be used as a platform for variant screening. RBD-foldon 2.2 is a novel antigen produced by fusing RBD with the trimerization domain Fibritin from Bacteriophage T4. Its amino acid sequence is based on the original …

The Effect Of A Vegetation Buffer On Sound Levels, Madison Cicha

Posters-at-the-Capitol

Noise pollution is a growing concern in the environmental health community. Unlike other common exposures, such as air and water pollution, noise is uniquely difficult for an individual to protect themselves from. Studies have shown multiple adverse health outcomes resulting from exposure to noise pollution, including hearing impairment, negative social behavior and annoyance, sleep disturbance, along with a strong association between noise pollution and high cardiovascular risk. It is important, then, to look for solutions to this exposure. This study is designed to assess the effects of a tree barrier in reducing traffic noise. Noise monitoring was conducted at Saint …

Decomposition Rates Of Organic Material Across Herbivore Treatments In A Nutrient-Rich Semi-Arid Sodic Savanna, L. L. Erasmus, H. Van Coller, S. Claassens, F. Siebert

IGC Proceedings (1977-2023)

Decomposition is a major determinant of terrestrial nutrient cycling and therefore an important regulator of ecosystem structure and function. It has been widely documented that large mammalian herbivores (LMH) act as a significant driver of changes to aboveground structure and modifications to edaphic properties. Little is known about the role of herbivory, and particularly the loss thereof, in mediating essential ecological processes in a herbivore-adapted system. The Nkuhlu exclosures, a large-scale, long-term exclusion experiment in the Kruger National Park, South Africa, provided an opportunity to explore the effects of herbivory and/or its long-term exclusion on decomposition and stabilisation of detrital …

Comparative Anatomical Study Of The Grasses In The Range Lands Of Kovilpatti, Tamil Nadu Of India, B. Makesh Kumar, J. Stephan, P. Kumar

IGC Proceedings (1977-2023)

Grasses are the crucial component of the rural rangeland ecology. Besides the morphological studies, micro-morphological characters such as stem anatomy were also investigated in this study. So as to get detailed understanding about variations during the summer and monsoon within species and also differentiating the species with the help of supplementary characters such as number of cortical layers and vascular bundles, xylem vessel length etc. In grass anatomy the characters are rather constant for some species and highly variable for others probably often due to the climatic variation. Still the characters are very helpful to differentiate and determine the genus. …

Stride-Level Analysis Of Mouse Open Field Behavior Using Deep-Learning-Based Pose Estimation., Keith Sheppard, Justin Gardin, Gautam S Sabnis, Asaf Peer, Megan Darrell, Sean P Deats, Brian Q Geuther, Cathleen Lutz, Vivek Kumar

Faculty Research 2022

Gait and posture are often perturbed in many neurological, neuromuscular, and neuropsychiatric conditions. Rodents provide a tractable model for elucidating disease mechanisms and interventions. Here, we develop a neural-network-based assay that adopts the commonly used open field apparatus for mouse gait and posture analysis. We quantitate both with high precision across 62 strains of mice. We characterize four mutants with known gait deficits and demonstrate that multiple autism spectrum disorder (ASD) models show gait and posture deficits, implying this is a general feature of ASD. Mouse gait and posture measures are highly heritable and fall into three distinct classes. We …

A Call For The Library Community To Deploy Best Practices Toward A Database For Biocultural Knowledge Relating To Climate Change, Martha B. Lerski

Publications and Research

Abstract

Purpose – In this paper, a call to the library and information science community to support documentation and conservation of cultural and biocultural heritage has been presented.

Design/methodology/approach – Based in existing Literature, this proposal is generative and descriptive— rather than prescriptive—regarding precisely how libraries should collaborate to employ technical and ethical best practices to provide access to vital data, research and cultural narratives relating to climate.

Findings – COVID-19 and climate destruction signal urgent global challenges. Library best practices are positioned to respond to climate change. Literature indicates how libraries preserve, share and cross-link cultural and scientific knowledge. …

Identifying Conifer Tree Vs. Deciduous Shrub And Tree Regeneration Trajectories In A Space-For-Time Boreal Peatland Fire Chronosequence Using Multispectral Lidar, Humaira Enayetullah, Laura Chasmer, Christopher Hopkinson, Dan Thompson, Danielle Cobbaert

Aspen Bibliography

Wildland fires and anthropogenic disturbances can cause changes in vegetation species composition and structure in boreal peatlands. These could potentially alter regeneration trajectories following severe fire or through cumulative impacts of climate-mediated drying, fire, and/or anthropogenic disturbance. We used lidar-derived point cloud metrics, and site-specific locational attributes to assess trajectories of post-disturbance vegetation regeneration in boreal peatlands south of Fort McMurray, Alberta, Canada using a space-for-time-chronosequence. The objectives were to (a) develop methods to identify conifer trees vs. deciduous shrubs and trees using multi-spectral lidar data, (b) quantify the proportional coverage of shrubs and trees to determine environmental conditions driving …

Associations Between Feeding Behaviors Collected From An Automated Milk Feeder And Neonatal Calf Diarrhea In Group Housed Dairy Calves: A Case-Control Study, Meridith H. Conboy, Charlotte B. Winder, Melissa C. Cantor, Joao H. C. Costa, Michael A. Steele, Catalina Medrano-Galarza, Taika E. Von Konigslow, Amanda Kerr, Dave L. Renaud

Animal and Food Sciences Faculty Publications

The objective of this case-control study was to determine if feeding behavior data collected from an automated milk feeder (AMF) could be used to predict neonatal calf diarrhea (NCD) in the days surrounding diagnosis in pre-weaned group housed dairy calves. Data were collected from two research farms in Ontario between 2017 and 2020 where calves fed using an AMF were health scored daily and feeding behavior data (milk intake (mL/d), drinking speed (mL/min), number of rewarded or unrewarded visits) was collected. Calves with NCD were pair matched to healthy controls (31 pairs) by farm, gender, and age at case diagnosis …

Heterologous Production Of Plant Secondary Metabolites Resveratrol And Caffeic Acid In E.Coli And Synechocystis Sp. Pcc 6803, Yong Xue

Theses and Dissertations

Caffeic acid and resveratrol are polyphenol compounds produced by plants as secondary metabolites to protect against biotic and abiotic stresses. In the last decades, numerous studies have demonstrated the beneficial effects of these compounds on human health as anticancer, antioxidant, anti-virus and anti-inflammatory agents. In plants, both caffeic acid and resveratrol are synthesized either from phenylalanine or tyrosine. Recently, heterologous production of phenylpropanoids using genetically engineered bacteria is gaining more and more attentions from industry because it can dramatically improve the yield with reduced costs. The major aim of the work is to explore the possibility of using Synechocystis or …

Pressure-Mediated Reflection Spectroscopy Criterion Validity As A Biomarker Of Fruit And Vegetable Intake: A 2-Site Cross-Sectional Study Of 4 Racial Or Ethnic Groups, Stephanie B Jilcott Pitts, Nancy E Moran, Qiang Wu, Lisa Harnack, Neal E Craft, Neil Hanchard, Ronny Bell, Stacey G Moe, Nevin Johnson, Justice Obasohan, Pamela L Carr-Manthe, Melissa N Laska

Children’s Nutrition Research Center Staff Publications

Background: Valid biomarkers of fruit and vegetable (FV) intake are needed for field-based nutrition research.

Objectives: To examine criterion-related validity of pressure-mediated reflection spectroscopy as a proxy measure of FV intake, using plasma carotenoids and self-reported FV and carotenoid intake as primary and secondary criterion measures, respectively.

Methods: Healthy adults 18-65 y of age, self-identifying as African American/black (n = 61), Asian (n = 53), white (n = 70), or Hispanic (n = 29), in North Carolina and Minnesota were recruited. Skin carotenoids were assessed via pressure-mediated reflection spectroscopy (Veggie Meter), skin melanin via spectrophotometer, and total plasma carotenoid concentration …

The Role Of Endoplasmic Reticulum Modifications In Protein Export In Plasmodium Falciparum, Alexander Polino

Arts & Sciences Graduate Student Theses and Dissertations

Following invasion of a host red blood cell (RBC), the malaria parasite Plasmodium falciparum undertakes a program of dramatic host cell renovation. The parasite exports hundreds of effectors into the RBC, altering its shape and rigidity, increasing its permeability to nutrients, and constituting an intracellular transport network that traffics adhesins to the RBC surface. These adhesins mediate binding of the parasitized RBC to the host vascular endothelium, allowing the parasite to avoid destruction in the spleen, but contributing to the pathogenesis of cerebral malaria. Most of these exported effectors bear a pentameric amino acid sequence that marks them for protein …

Chromomap: An R Package For Interactive Visualization Of Multi-Omics Data And Annotation Of Chromosomes, Lakshay Anand, Carlos M. Rodriguez Lopez

Horticulture Faculty Publications

BACKGROUND: The recent advancements in high-throughput sequencing have resulted in the availability of annotated genomes, as well as of multi-omics data for many living organisms. This has increased the need for graphic tools that allow the concurrent visualization of genomes and feature-associated multi-omics data on single publication-ready plots.

RESULTS: We present chromoMap, an R package, developed for the construction of interactive visualizations of chromosomes/chromosomal regions, mapping of any chromosomal feature with known coordinates (i.e., protein coding genes, transposable elements, non-coding RNAs, microsatellites, etc.), and chromosomal regional characteristics (i.e. genomic feature density, gene expression, DNA methylation, chromatin modifications, etc.) of organisms …

The Parental Relationship Agreement And Its Implications For Family And Community Security In The Emirate Of Sharjah 2022, Abdalla Alhayayi

Journal of Police and Legal Sciences

This study aimed to shed light on the Parental Relationship Agreement issued by His Highness Sheikh Dr. Sultan bin Muhammad Al Qasimi, Ruler of Emirate of Sharjah and a member of the Federal Supreme Council of the United Arab Emirates, the impact of this agreement on family and community security in the Emirate of Sharjah. The study used the descriptive analytical approach, and relied on the electronic questionnaire as a tool for data collection. From the study community, who are the employees of the Department of Social Services and the Family Court in the Emirate of Sharjah, which are charged …

Smokejumper Obituary: Hawley, Hal (North Cascades 1967), National Smokejumper Association

Smokejumper Obituaries

No abstract provided.

Smokejumper Obituary: Boucher, Albert N. “Al” (Cave Junction 1949), National Smokejumper Association

Smokejumper Obituaries

No abstract provided.

Smokejumper Obituary: Siemens, Roger M. (Missoula 1959), National Smokejumper Association

Smokejumper Obituaries

No abstract provided.

Massively Parallel Assessment Of The Functional Effects Of De Novo Autism Spectrum Disorder Patient-Derived Genetic Variants In 3’ Untranslated Regions, Tomas Lagunas Jr

Arts & Sciences Graduate Student Theses and Dissertations

Human genetic studies have identified a large number of patient-derived de novo variants in presumptive regulatory regions of the genome that pose a challenge for interpretation of their effects since the impact of regulatory variants is highly dependent on the cellular context. In particular, current investigations have made significant investments in sequencing the genomes of autism spectrum disorder (ASD) families as a diagnostic method and to further understand the genetic architecture of this complex neurodevelopmental disease. These studies have revealed an enrichment of mutations in the untranslated regions (UTRs) of genes, which are noncoding regulatory regions, in ASD genomes. Assessment …