Life Sciences Commons^™

Selective Gsk3Β Inhibition Mediates An Nrf2-Independent Anti-Inflammatory Microglial Response, Mohamed H. Yousef

Theses and Dissertations

Glycogen Synthase Kinase 3 (GSK3) is associated with the proinflammatory phenotype of microglia and has been shown to act in concert with Nuclear factor kappa B (NF-κB). . GSK3 is also a suppressor of Nuclear factor erythroid 2-related factor 2 (Nrf2), the principal regulator of redox homeostasis. Agreeing with the oxidative paradigm of aging, Nrf2 is often deregulated in parainflammatory and neurodegenerative diseases. In this study, we aimed to explore a multimodal disease-modifying utility of GSK3 inhibition, beyond neuronal proteopathologies, Furthermore, we aimed to underscore the difference in therapeutic value between the two GSK3 paralogs by isoform-selective chemical inhibition.

The …

Repurposing Metformin And Antifolates For The Treatment Of Hepatocellular Carcinoma, Sherouk Mohamed Tawfik

Theses and Dissertations

Hepatocellular carcinoma (HCC), one of the most prevalent types of cancers worldwide, continues to maintain high levels of resistance to standard therapy. As clinical data revealed poor response rates, the need for developing new methods has increased to improve the overall wellbeing of patients with HCC. Due to its safety, wide availability and previously reported anti-cancer effects, metformin (MET) serves to be a possible therapeutic agent when combined with other well-known anti-cancer agents. The aim of this study was to investigate the potential anti-cancer effects of MET, an anti-diabetic agent, when combined with two antifolate drugs: trimethoprim (TMP) or methotrexate …

Conformational Flexibility And Local Frustration In The Functional States Of The Sars-Cov-2 Spike B.1.1.7 And B.1.351 Variants: Mutation-Induced Allosteric Modulation Mechanism Of Functional Dynamics And Protein Stability, Gennady M. Verkhivker

Mathematics, Physics, and Computer Science Faculty Articles and Research

Structural and functional studies of the SARS-CoV-2 spike proteins have recently determined distinct functional states of the B.1.1.7 and B.1.351 spike variants, providing a molecular framework for understanding the mechanisms that link the effect of mutations with the enhanced virus infectivity and transmissibility. A detailed dynamic and energetic analysis of these variants was undertaken in the present work to quantify the effects of different mutations on functional conformational changes and stability of the SARS-CoV-2 spike protein. We employed the efficient and accurate coarse-grained (CG) simulations of multiple functional states of the D614G mutant, B.1.1.7 and B.1.351 spike variants to characterize …

Gene-Gene Interaction Of Ahrwith And Within The Wntcascade Affects Susceptibility To Lung Cancer, Albert Rosenberger, Nils Muttray, Rayjean J Hung, David C Christiani, Neil E Caporaso, Stig E Bojesen, Demetrios Albanes, Melinda C Aldrich, Adonina Tardon, Guillermo Fernández-Tardón, Gad Rennert, John K Field, Michael P A Davies, Triantafillos Liloglou, Lambertus A Kiemeney, Philip Lazarus, Bernadette Wendel, Aage Haugen, Shanbeh Zienolddiny, Stephen Lam, Matthew B Schabath, Angeline S Andrew, Eric J Duell, Susanne M Arnold, Gary E Goodman, Chu Chen, Jennifer A Doherty, Fiona Taylor, Angela Cox, Penella J Woll, Angela Risch, Thomas R Muley, Mikael Johansson, Paul Brennan, Maria Teresa Landi, Sanjay S Shete, Christopher I Amos, Heike Bickeböller, Integral-Ilcco Consortium

Faculty, Staff and Student Publications

Background: Aberrant Wnt signalling, regulating cell development and stemness, influences the development of many cancer types. The Aryl hydrocarbon receptor (AhR) mediates tumorigenesis of environmental pollutants. Complex interaction patterns of genes assigned to AhR/Wnt-signalling were recently associated with lung cancer susceptibility.

Aim: To assess the association and predictive ability of AhR/Wnt-genes with lung cancer in cases and controls of European descent.

Methods: Odds ratios (OR) were estimated for genomic variants assigned to the Wnt agonist and the antagonistic genes DKK2, DKK3, DKK4, FRZB, SFRP4 and Axin2. Logistic regression models with variable selection were trained, validated and tested to predict lung …

Identifying The Metabolic Signatures Of Ppard-Overexpressing Gastric Tumors, Shivanand Pudakalakatti, Mark Titus, José S Enriquez, Sumankalai Ramachandran, Niki M Zacharias, Yi Liu, Xiangsheng Zuo, Pratip K Bhattacharya

Faculty, Staff and Student Publications

Peroxisome proliferator-activated receptor delta (PPARD) is a nuclear receptor known to play an essential role in regulation of cell metabolism, cell proliferation, inflammation, and tumorigenesis in normal and cancer cells. Recently, we found that a newly generated villin-PPARD mouse model, in which PPARD is overexpressed in villin-positive gastric progenitor cells, demonstrated spontaneous development of large, invasive gastric tumors as the mice aged. However, the role of PPARD in regulation of downstream metabolism in normal gastric and tumor cells is elusive. The aim of the present study was to find PPARD-regulated downstream metabolic changes and to determine the potential significance of …

Chris Burkhardt – Covid 19 Oral History Project, Chris Burkhardt, Institute Of Child Nutrition. Child Nutrition Archives.

Oral History Project (all interviews)

During 2022 the Child Nutrition Archives conducted multiple interviews investigating how child nutrition professionals dealt with the Covid 19 pandemic and its effects on the operations of school feeding programs. This interview examines the experiences of Chris Burkhardt, who is the executive director of school nutrition for the Cleveland (Ohio) Metropolitan School District.

Real-World Applications For Virtual Fences – What Are Potential Benefits For Conservation?, F. Riesch, M. Komainda, J. Horn, J. Isselstein

IGC Proceedings (1977-2023)

Livestock grazing can enhance biodiversity and ecosystem services in agricultural landscapes. In many parts of Europe, however, grazing has lost its importance, especially in the dairy sector. Large proportions of permanent grassland have been converted to arable land or intensified by fertilization and frequent defoliation. The disappearance of large herbivores and extensively grazed pastures contributes to the loss of structural, functional and biological diversity and ecosystem services. Modern technologies, which circumvent the cost- and labour-intensive installation of physical fences, could facilitate a precise spatio-temporal management of livestock and promote grazing. We reviewed the literature on the state-of-the-art of virtual fencing, …

Sahiwal Breed In Pastoral Production Systems In Kenya: Future Roles And Existing Gaps In Knowledge And Supportive Technical Logistics; A Review, Samson I. Mwangi, E. D. Ilatsia

IGC Proceedings (1977-2023)

Sahiwal genetic resources is an integral part of the ASALs economy of Kenya. Sahiwal is the largest single dual purpose cattle breed kept by the pastoral communities in the ASALs where livestock products are the main drivers of food and nutrition security, and a source of income to support other household obligations. It was introduced in Kenya to upgrade the low performing small east African Zebu because of its superior productive and reproductive performance. The promising results of this upgrading programme led to the breed gaining preference over the local Zebu among the Maasai pastoralists. Despite the impressive results from …

Potential Of Croton Megalocarpus Nut As An Alternative Protein Supplement For Feeding Of Ruminants In The Tropics, E. K. Njoroge, C. C. Wambui, C. B. Wasike

IGC Proceedings (1977-2023)

Croton megalocarous is a tree native to the arid and semi-arid rangelands of East Africa that produces nuts reported to contain high crude protein (CP). However, they are often neglected as a potential feed due to lack of information on how best to utilize them. This study was conducted to evaluate the chemical composition and in sacco dry matter (DM) degradability of four forms of croton namely whole nut (WN), peeled nut (PN), De-husked nut (DhN) and Defatted seed (DfS). DM of all forms was above 89%, ash content was highest in WN (5.9%) and least in PN (2.2%). Crude …

Polyphenol Concentration Of Native Plant Species, And Its Effect On Blood Antioxidant Capacity In Grazing Cattle In A Species-Rich Vegetation In Japan, S. Ogura, A. Tamura, H. Kakihara, T. Shishido, K. Shimoda

IGC Proceedings (1977-2023)

It is known that polyphenols in plants have a high antioxidant capacity. However, there is scarce information on its concentration in native plants and the effect of antioxidant capacity to grazing animals. In this study, polyphenol concentration of available plants was investigated in a species-rich grazing area in north-eastern district of Japan. In addition, polyphenol concentration in blood serum of grazing cattle was also measured. Eight beef cows grazed in a grazing area (hill pasture 3.1 ha; forest 16.9 ha) from late spring to mid-autumn (142 days), and four cows among the eight grazed at an orchardgrass pasture in mid-summer …

Evaluation Of Dietary Roughage Inclusion In A Single Or Two-Diet System For Backgrounding And Finishing, Thomas G. Hamilton, Warren Rusche, Zachary K. F. Smith

SDSU Beef Day 2022 Summary Publication

Study Description:

Pre-conditioned crossbred beef steers (n = 46; initial shrunk [4%] BW = 621 ± 89.1 lbs) were used in a 210-d grow-finish experiment at the Ruminant Nutrition Center (RNC) in Brookings, SD. Steers were fed once daily, and bunks were managed according to a slick bunk management system. Cattle were fed in 25 × 25 ft concrete surface pens (n = 10 pens; 5 pens/treatment) with 25 linear ft of bunk space and heated, concrete, continuous flow waterers. Treatments included: 1) A single-diet program (targeted a 59 Mcal/cwt NEg diet fed for 210-d; 1D) or 2) two-diet program …

Genetic Interaction Between Mfrp And Adipor1 Mutations Affect Retinal Disease Phenotypes, Navdeep Gogna, Sonia Weatherly, Fuxin Zhao, Gayle B. Collin, Jai Pinkney, Lisa Stone, Juergen K. Naggert, Gregory W. Carter, Patsy M. Nishina

Faculty Research 2022

Adipor1^tm1Dgen and Mfrp^rd6 mutant mice share similar eye disease characteristics. Previously, studies established a functional relationship of ADIPOR1 and MFRP proteins in maintaining retinal lipidome homeostasis and visual function. However, the independent and/or interactive contribution of both genes to similar disease phenotypes, including fundus spots, decreased axial length, and photoreceptor degeneration has yet to be examined. We performed a gene-interaction study where homozygous Adipor1^tm1Dgen and Mfrp^rd6 mice were bred together and the resulting doubly heterozygous F1 offspring were intercrossed to produce 210 F2 progeny. Four-month-old mice from all nine genotypic combinations obtained in the F2 generation …

Smokejumper Obituary: Bilbao, Julio Bernardo (Idaho City 1964), National Smokejumper Association

Smokejumper Obituaries

No abstract provided.

Performance Of Lora-Wan Sensors For Precision Livestock Tracking And Biosensing Applications, S. Nyamuryekung’E, Andrés F. Cibils, R. E. Estell, M. Funk, M. M. Mcintosh, A. Cox, S. A. Utsumi, H. Cao, L. Boucheron, Q. Gong, H. Chen, S. Spiegal, G. Duff, V. Gouvea, C. B. Brandani

IGC Proceedings (1977-2023)

This study investigated the integration of Long Range Wide Area Network (LoRa WAN) communication technology and sensors for use as Internet of Things (IoT) platform for Precision Livestock-Farming (PLF) applications. The research was conducted at New Mexico State University’s Clayton Livestock Research Centre. The functionality of LoRA WAN communication technology and performance of LoRa WAN motion and GPS sensors were tested using static sensors that were placed either, a) outdoors and at incremental distances from the LoRa WAN gateway antenna (Field, n=6), or b) housed indoors and close to the same LoRa WAN gateway antenna (Indoor, n=5). Accelerometer data, reported …

Pasture Management Relevant Behaviour Of Yaks (Bosgrunniens), O. Kaufmann, A. Tolebekova

IGC Proceedings (1977-2023)

After a decline in yak populations in Central Asia, efforts have been made for several years to rebuild them. In that context, the ecological demands of the alpine landscape must be taken into account. The aim of the studies was to analyse the behaviour of yak cows in short time intervals and to deduce which sub-areas the yaks prefer more or less strongly, which altitudes in the mountains the animals visit in the course of a year, which distances they cover in summer and winter and which factors influence their grazing behaviour. The investigations were carried out on yaks in …

Herbage And Livestock Responses For N-Fertilized And Grass-Legume Grazing Systems, D. M. Jaramillo, José C. B. Dubeux Jr., L. M. D. Queiroz, L. Garcia, E. R. S. Santos

IGC Proceedings (1977-2023)

Forage legumes provide an alternative N source in grazing systems. The objective was to evaluate plant and animal responses in N-fertilized or grass-legume-based systems under continuous stocking during winter and summer, from 2016-2019. The three treatments consisted of year-round forage systems including winter and summer forage components. The first system (Grass+N) included N-fertilized (112 kg N ha^-1 yr^-1) ‘Argentine’ bahiagrass (Paspalum notatum) pastures during the summer, overseeded with a mixture (56 kg ha^-1 of each) of ‘FL 401’ cereal rye (Secale cereale) and ‘RAM’ oat (Avena sativa) during winter with …

New Forms Of Pastoral Mobility Among Sheep Herders In The Algerian Steppe; The Case Of Pastoralists Of Djelfa, Dihia Gaci, Jean-Pierre Boutonnet

IGC Proceedings (1977-2023)

The recent socio-economic, political and climatic changes experienced by the Algerian steppe for the last decades have led to profound changes in pastoral mobility practices. This study describes the new mobility practices as currently adopted by the breeders of the Djelfa region. The realization of 59 semi-directive surveys was conducted between 2014 and 2016 among breeders in different reception areas. Practices related to the distance travelled during an annual cycle, the transhumance calendar and itinerary and the passage or not in the homeland are diverse. These practices of mobility can vary from one year to another or during the life …

The Poultry Industry And Its Environmental Impacts, Mohamed Taha El-Barbary M.A. Omar

Archived Theses and Dissertations

No abstract provided.

Using Single Cell Type Proteomics To Identify Al-Induced Proteomes In Outer Layer Cells And Interior Tissues In The Apical Meristem/Cell Division Regions Of Tomato Root-Tips, Jesse Potts, Hui Li, Yaoguo Qin, Xingbo Wu, Dafeng Hui, Kamal Al Nasr, Suping Zhou, Yang Yong, Tara Fish, Jiping Liu, Theodore W. Thannhauser

Biology Faculty Research

Aluminum (Al) toxicity primarily targets the root tips, inhibiting root growth and function and leading to crop yield losses on acidic soils. Previously we reported using laser capture microdissection (LCM) proteomics to identify Al-induced proteins in the outer layer cells in the transitional zone of tomato root-tips. This study aims to further characterize Al-induced proteomic dynamics from the outer to interior tissues, thus providing a panoramic view reflecting Al resistance in the root tip as a whole in tomatoes. Three types of cells were isolated via LCM from the basal 350–400 μm (below cell elongation regions) of root tips using …

Surveillance Of Rnase P, Pmmov, And Crassphage In Wastewater As Indicators Of Human Fecal Concentration Across Urban Sewer Neighborhoods, Louisville, Kentucky., R. H. Holm, M. Nagarkar, R. A. Yeager, D. Talley, A. C. Chaney, J. P. Rai, A. Mukherjee, S. N. Rai, A. Bhatnagar, T. Smith

Faculty and Staff Scholarship

Wastewater surveillance has been widely used as a supplemental method to track the community infection levels of severe acute respiratory syndrome coronavirus 2. A gap exists in standardized reporting for fecal indicator concentrations, which can be used to calibrate the primary outcome concentrations from wastewater monitoring for use in epidemiological models. To address this, measurements of fecal indicator concentration among wastewater samples collected from sewers and treatment centers in four counties of Kentucky (N = 650) were examined. Results from the untransformed wastewater data over 4 months of sampling indicated that the fecal indicator concentration of human ribonuclease P (RNase …

Dose Effects Of Encapsulated Butyric Acid And Zinc On Beef Feedlot Steer Growth Performance, Carcass Characteristics, And Dietary Net Energy Utilization, Forest L. Francis, Erin R. Gubbels, Thomas G. Hamilton, Warren Rusche, Doug Lafleur, Jerilyn E. Hergenreder, Zachary K. F. Smith

SDSU Beef Day 2022 Summary Publication

Study Description:

Steers (n = 272; shrunk BW = 794 ± 163 pounds) were assigned to one of four dietary treatments in a 143.5 d feedlot finishing trial: 0 g BPZ/ kg diet dry matter (DM) (CON), 1 g BPZ/ kg diet DM (1BPZ), 2 g BPZ/ kg diet DM (2BPZ), or 3 g BPZ/ kg diet DM (3BPZ). Carcass data and liver health outcomes were collected, and feedlot growth performance data and efficiency of dietary net energy utilization were calculated on a carcass-adjusted basis.

A Review Of Landcover Classification With Very-High Resolution Remotely Sensed Optical Images—Analysis Unit, Model Scalability And Transferability, Rongjun Qin, Tao Liu

Michigan Tech Publications, Part 1

As an important application in remote sensing, landcover classification remains one of the most challenging tasks in very-high-resolution (VHR) image analysis. As the rapidly increasing number of Deep Learning (DL) based landcover methods and training strategies are claimed to be the state-of-the-art, the already fragmented technical landscape of landcover mapping methods has been further complicated. Although there exists a plethora of literature review work attempting to guide researchers in making an informed choice of landcover mapping methods, the articles either focus on the review of applications in a specific area or revolve around general deep learning models, which lack a …

A Review Of Landcover Classification With Very-High Resolution Remotely Sensed Optical Images—Analysis Unit, Model Scalability And Transferability, Rongjun Qin, Tao Liu

Michigan Tech Publications, Part 1

As an important application in remote sensing, landcover classification remains one of the most challenging tasks in very-high-resolution (VHR) image analysis. As the rapidly increasing number of Deep Learning (DL) based landcover methods and training strategies are claimed to be the state-of-the-art, the already fragmented technical landscape of landcover mapping methods has been further complicated. Although there exists a plethora of literature review work attempting to guide researchers in making an informed choice of landcover mapping methods, the articles either focus on the review of applications in a specific area or revolve around general deep learning models, which lack a …

Data Validating The Use Of Rubidium As A Non-Radioactive Tracer For The Localised Proliferation Of Wheat Roots In Acidic Or Limed Subsoil, Paul M. Damon, Gaus Azam, Chris Gazey, Craig Scanlan, Zed Rengel

Grain and Other Field Crops Research Articles

Existing technologies for lime (CaCO3) incorporation into acidic field soils result in the heterogeneous distribution of limed and acidic soil sections. In a study characterising the response of wheat (Triticum aestivum L.) to the amendment of an acidic soil profile with vertically limed slots[1], elucidation of the dynamics of root proliferation within the acidic and limed soil sections was a prerequisite to understanding the mechanisms driving the above-ground responses. Rubidium (Rb) has been used widely as a non-radioactive tracer for root activity[2] in soil. However, the contrasting pH in a heterogeneously limed soil profile and related aluminium toxicity effects …

Impact Of Frontline Treatment Approach On Outcomes In Patients With Secondary Aml With Prior Hypomethylating Agent Exposure, Nicholas J Short, Sangeetha Venugopal, Wei Qiao, Tapan M Kadia, Farhad Ravandi, Courtney D Dinardo, Marina Konopleva, Gautam Borthakur, Elizabeth J Shpall, Uday Popat, Richard E Champlin, Rohtesh Mehta, Gheath Al-Atrash, Betul Oran, Elias Jabbour, Guillermo Garcia-Manero, Ghayas C Issa, Guillermo Montalban-Bravo, Musa Yilmaz, Abhishek Maiti, Hagop Kantarjian

Faculty, Staff and Student Publications

BACKGROUND: Treated secondary acute myeloid leukemia (ts-AML)-i.e., AML arising from a previously treated antecedent hematologic disorder-is associated with very poor outcomes. The optimal frontline treatment regimen for these patients is uncertain.

METHODS: We retrospectively analyzed 562 patients who developed AML from preceding myelodysplastic syndrome or chronic myelomonocytic leukemia for which they had received a hypomethylating agent (HMA). Patients with ts-AML were stratified by frontline AML treatment with intensive chemotherapy (IC, n = 271), low-intensity therapy (LIT) without venetoclax (n = 237), or HMA plus venetoclax (n = 54).

RESULTS: Compared with IC or LIT without venetoclax, HMA plus venetoclax resulted …

Pastoralism, Social, Gender, And Policy Issues, Nahid Naghizadeh

IGC Proceedings (1977-2023)

Nearly half of the Earth's land surface is classified as rangelands. Rangeland's health and productivity are directly critical to the livelihoods, cultures, and resilience of more than 500 million people worldwide, many of whom are indigenous peoples who depend on rangelands. Pastoralism is very diverse; it can be found in all continents, from the drylands of Africa and the Arabian Peninsula to the highlands of Asia and Latin America, or the tundra in the circumpolar zones, and in particular where crop cultivation is physically limited. Pastoralism supports several hundred million households worldwide and manages one billion animals, including camels, cattle, …

Ecological Science Infrastructure For Sustainability Transformations In Rangelands, Brandon T. Bestelmeyer, J. E. Herrick, J. R. Brown, D. M. Browning, B. Densambuu, E. Elias, S. E. Mccord, H. R. Peinetti, S. Spiegal, N. P. Webb, J. C. Williamson

IGC Proceedings (1977-2023)

Sustainability transformations—deliberate and radical shifts in values, governance, and management regimes to achieve sustainability—are needed in rangelands as in other components of the Earth system. We review four concepts comprising an ecological science infrastructure to support such transformations. The foundation is standard measurement of rangeland conditions in the field, especially vegetation and soil properties that underpin the environmental aspects of sustainability. Big data resources, especially gridded spatial datasets produced by models and remote sensing, can be combined with field data and computational approaches to upscale information about rangeland conditions and produce additional indicators of ecosystem functions and services. State and …

Annual Ryegrass Pasture For Dairy Cows Receiving Total Mixed Ration, M. Civiero, L. H. Schaitz, C. P. Ferris, Henrique M. N. Ribeiro-Filho

IGC Proceedings (1977-2023)

The inclusion of herbage in the diet of medium yielding dairy cows offered a total mixed ration (TMR) may be beneficial. This study, which involved mid lactation dairy cows, examined the effect of partial replacement of a TMR with annual temperate pasture. Treatments were ad libitum TMR (TMR₁₀₀), 75% ad libitum TMR + ryegrass (Lolium multiflorum ‘Maximus’) (TMR₇₅), and 50% ad libitum TMR + ryegrass (TMR₅₀). Twelve multiparous Holstein and F1 Jersey × Holstein cows were divided into six homogeneous groups, taking account of milk production (26.6 ± 4.5 kg/day), days in milk …

A Guava-Based Hortipasture System For Mitigating Climate Change And Sustaining Fodder & Fruit Supply In Semi-Arid Regions Of India, Kamini Gautam, Sunil Kumar, Amit Kumar Singh, Avijit Ghosh, Manjanagouda S. S., R. V. Kumar

IGC Proceedings (1977-2023)

Hortipasture systems have huge potential to mitigate climate change via sequestering carbon along with sustaining fodder and fruit supply especially in semi-arid regions. Therefore to evaluate climate change mitigation, fruit and fodder production potential of 10 year old rainfed based Hortipasture system (Psidium guajava (Guava) + Cenchrus ciliaris + Stylosanthes hamata) established at Central Research Farm of ICAR-Indian Grassland and Fodder Research Institute (Bundelkhand region, Jhansi, Uttar Pradesh, India), carbon stock of tree and under storey pasture components was quantified along with fruit and forage production. The experimental site is drought prone semi arid region characterized by poor …

Free-Enterprise Farming On Grasslands In Central Nsw, Australia, Des Green, Stuart Green, Ted Wolfe, Jo Richardson, Rob Eccles

IGC Proceedings (1977-2023)

A pathway is described in developing and improving the pastures on family-owned sheep and beef properties at sites near Blayney in central NSW. Initially, the twin approach of sowing perennial grasses, predominantly phalaris (Phalaris aquatica) with subterranean clover (Trifolium subterraneum) plus the recommended addition of superphosphate fertiliser, was closely followed but within a decade ill-thrift in pastures and livestock occurred. Once the core problem of soil acidity was recognised, steps were taken to overcome this constraint with applications of lime. However, an additional modification involving the application of gypsum with lime had to be sorted out …