Life Sciences Commons^™

Accelerating The Discovery Of The Genetic And Molecular Bases Of Neurodevelopmental Disorders In Vivo Using Breasi-Crispr, Claire Mae Kittock

Dissertations and Theses

Many neurodevelopmental disorders occur due to perturbations in prenatal brain development. More genetic variants associated with neurodevelopmental disorders are continuously being identified. However, there is a lack of understanding of the pathogenic mechanisms. Here, we describe a novel application of the technique Breasi-CRISPR in modeling specific patient variants in the embryonic mouse brain. Breasi-CRISPR is a highly efficient system combining in utero electroporation with CRISPR-CAS9 genome editing. We hypothesized that Breasi-CRISPR would be sufficient at inserting patient analogous variants into endogenous loci to be used as a new paradigm for modeling neurodevelopmental disorders. We aimed to test if Breasi-CRISPR could …

The Effect Of Different Nutrient Concentrations For Hydroponically Grown Pothos, Keaton Roether

A with Honors Projects

An experiment where Pothos plants are grown in three different solutions of water, NPK diluted into water, tap water, and distilled water. Data is recorded for multiple weeks and is then compiled into a comprehensible lab report of a minimum of 1000 words with graphs and data.

Crispr-Cas9: Transforming Biomedical Technology Through Gene Editing, Zaina Issa

A with Honors Projects

The research paper describe the gene editing technique CRISPR-Cas9 and the implications of its use in different fields, including medicine and agriculture.

The Neuroprotective And Synergistic Effects Of Carbamoylated Erythropoietin And Insulin-Like Growth Factor-1 In Alzheimer's Disease, Morgan Jean Rothschadl

Dissertations and Theses

Alzheimer’s disease (AD) is a prominent neurodegenerative disease characterized by a decline in cognitive functioning. While current FDA-approved therapeutics exist, they fall short in creating significant and long-lasting improvements to cognition. A newer approach using neurotrophic factors has shown promising effects on improving cognition in clinical trials. Neurotrophic factors are secreted proteins that have multimodal effects in the brain, including supporting cell survival and functioning. Two neurotrophic factors of interest are carbamoylated erythropoietin (CEPO) and insulin-like growth factor-1 (IGF-1), and the goal of this study was to understand the synergistic and neuroprotective effects of CEPO and IGF-1 in AD. We …

Avian Use Of Early Successional Riparian Forest Along The Missouri River: What Do We Lose By Managing For Plovers And Terns?, Stephanie Nefas

Dissertations and Theses

Riparian ecosystems provide habitat for birds throughout their annual cycle, yet flow regulation along major rivers like the Missouri River has reduced early successional riparian forests. Following historic flooding in 2011, set-aside areas within the Missouri National Recreational River (MNRR) were established to allow natural riparian vegetation succession on newly created sandbars. I evaluated the value of these early successional forests for birds over three breeding seasons (2018–2020) and four migration periods (2020–2021). During migration, standardized point counts documented 7,462 individual birds representing 114 species across 828 surveys. Avian diversity was positively correlated with vertical habitat complexity, with the canopy …

Forage News [2026-04], University Of Kentucky

Forage News

No abstract provided.

Forage News [2026-02], University Of Kentucky

Forage News

No abstract provided.

Forage News [2026-01], University Of Kentucky

Forage News

No abstract provided.

Long Acting Beta2-Adrenergic Receptor Agonists Reverse Diabetic Nephropathy And Liver Steatosis In Murine Models Of Diabetes And Are Associated With Protection In Retrospective Human Studies, Brennan Winkler

MUSC Theses and Dissertations

Diabetes Mellitus (DM) affects 13.9% of men and 14.3% of women worldwide, a total of approximately 828 million people. Patients with diabetes are at risk of several complications including diabetic nephropathy (DN) and metabolic-associated steatohepatitis (MASH). These diseases eventually lead to end stage kidney disease (ESKD) and liver failure requiring dialysis and organ transplantation. In addition, diabetes exacerbates disease severity of autosomal dominant polycystic kidney disease (ADPKD), the most common potentially lethal genetic disease to affect humans with a prevalence of 1 in 500-1000. Therefore, a therapeutic approach that can attenuate organ damage and restore function in patients with DM, …

The Impact Of The Different Vdac Isoforms On Hbv Replication And Hbx Localization, Cody Mcumber

MUSC Theses and Dissertations

Chronic hepatitis B virus (HBV) infection is the leading cause of hepatocellular carcinoma worldwide. Despite the availability of a preventative vaccine, there are approximately 254 million people living with a chronic HBV infection. There are 9 known HBV genotypes, each with their own clinical impacts and manifestation in infected patients. HBx is the only regulatory protein encoded by the HBV genome and is required for efficient viral replication. HBx has many effects in infected hepatocytes besides known effects on HBV replication, such as localization to the mitochondria. HBx is also known to have impacts on mitochondrial membrane potential, mitochondrial permeability …

Effect Of Corn Harvest With A Chopping Head On Performance Of Cattle Grazing Corn Residue, Conner K. Biehler, Mary E. Drewnoski, Jim C. Macdonald

Nebraska Beef Cattle Reports

Summary with Implications

The effects of harvesting corn using a chopping vs a standard, non-chopping combine head on steer performance when grazing corn residue were evaluated. Steers were supplemented with dry distillers grains at 0.6% of initial body weight. The amount of corn residue mass and the quality of the husk did not differ between harvest methods. Steer gains were over twice as high in year 2 (2.10 lb/d) than year 1 (0.76 lb/d), potentially due to greater husk digestibility and milder winter conditions. Corn residue harvested with a standard combine head resulted in a slightly higher average daily gain …

Artificial Insemination Of Beef Heifers With Multi-Sire Semen, Dempster M. Christenson, Jordan M. Thomas, Daniel J. Kelly, Rick N. Funston

Nebraska Beef Cattle Reports

Summary with Implications

This study compared pregnancy rates of beef heifers artificially inseminated with multi-sire semen to single-sire semen. It was hypothesized pregnancy rates resulting from multi-sire semen would be increased compared to single-sire semen. Heifers were artificially inseminated with semen from one of three sires or semen from a combination of the same three sires. Pregnancy rates did not significantly differ. Paternity testing suggests sire parentage can be unequal when semen is mixed from multiple sires. In summary, similar pregnancy rates were observed using single-sire and multi-sire semen, but progeny may have unequal sire representation.

Supplementing Monensin And Rumen Undegradable Protein To Late-Gestation Heifers, Dempster M. Christenson, Rick N. Funston

Nebraska Beef Cattle Reports

Summary with Implications

This study evaluates the effects of supplementing late-gestation heifers with rumen-undegradable protein and monensin on weight gain, feed efficiency, and reproductive performance, and subsequent impact on progeny. We hypothesized that supplementation would enhance growth and feed efficiency, ultimately improving cow and calf performance. Previously synchronized artificially inseminated heifers were fed ad libitum hay for 89 d prior to calving. Feed was top dressed with chelated mineral with or without monensin, dried distillers’ grains, or dried distillers’ grains and monensin. Supplementation with rumen undegradable protein increased growth rate and decreased calving difficulty with no further effects on cow …

Effect Of Feeding Whole Soybeans, Roasted Soybeans, Or Distillers Grains In Silage-Based Growing Beef Diets, Melissa K. Bausch, Rebecca Mcdermott, Jim C. Macdonald, Pablo L. Loza, Galen E. Erickson

Nebraska Beef Cattle Reports

Summary with Implications

An individual feeding experiment was conducted to evaluate the effect of feeding raw whole soybeans (WSB), roasted whole (RSB) soybeans or distillers grains (MDGS) at three inclusions (7%, 14%, or 21% of diet dry matter) on growing performance, compared to a common control diet (0%) with urea. The greater inclusions of roasted soybeans or distillers grains increased gain and efficiency in growing cattle. Cattle fed the 21% inclusion of roasted soybeans were the most efficient, likely due to the protected protein increasing rumen undegradable protein content, and the additional energy from the higher levels of fat and …

Live Biomass Estimation With Canopeo In Rangeland Systems, Hannah K. Allen, Mitchell B. Stephenson, Daniel R. Uden

Nebraska Beef Cattle Reports

Summary with Implications

This project evaluated Canopeo, a free smartphone app that estimates fractional green canopy cover (FGCC), as a tool for monitoring aboveground biomass in Nebraska Sandhills rangelands. Live biomass was significantly higher in August than in June with some variability by year. FGCC showed a moderately strong relationship with current-year live biomass collected in either June or August. These results indicate that, in addition to producers’ field knowledge, Canopeo images taken with a smartphone or digital camera can offer a quick, visual estimate of forage conditions. This method may help reduce the need for labor intensive and destructive …

Effect Of An Accelerated Adaptation Protocol On Performance And Health Of Feedlot Steers Drenched With Lactipro, Rebecca L. Mcdermott, Samuel E. Loy, Isaque F. Vicci, Mark Corrigan, Braden C. Troyer, Galen E. Erickson, Jim C. Macdonald

Nebraska Beef Cattle Reports

Summary with Implications

A finishing study evaluated the effects of an accelerated adaptation protocol in conjunction with Lactipro on performance and health of steers. Five adaptation programs were evaluated with steers adapted to a finishing diet in 21, 14, 7, or 0 days. An additional accelerated adaptation program provided intensive feed delivery with 0-d adaptation. Cattle assigned to the 21-d and 0-d adaptations gained faster and consequently had greater HCW compared to the 7-d and 14-d adaptation, resulting in a quadratic response for ADG and HCW. The 0-d adaptation with intensive feed delivery had similar ADG and HCW compared to …

Energy Value Of High-Moisture Corn In Feedlot Cattle Diets Derived Through Meta-Analysis, Alfredo Dicostanzo

Nebraska Beef Cattle Reports

Summary with Implications

Cattle feeders or their nutritionists who rely on high-moisture corn (HMC) use a standard adjustment to energy content of the diet based on observation or previous research reports. Because moisture content of HMC is the result of many field and harvest factors, a common adjustment to the energy value of HMC applied to all conditions may not be appropriate. The hypothesis that moisture content of HMC determines its energy concentration was tested. Through a meta-analysis, it was determined that feed intake was depressed while energy content of HMC increased at greater moisture content. A simple method to …

Effects Of Feeding Raw Soybeans Or Roasted Soybeans To Feedlot Steers, Melissa K. Bausch, Jim C. Macdonald, Pablo L. Loza, Galen E. Erickson

Nebraska Beef Cattle Reports

Summary with Implications

A feedlot study compared feeding raw or roasted soybeans, soybean meal, or distillers grains to a dry-rolled corn with urea control on finishing cattle performance and carcass traits. Cattle fed roasted soybeans had improved marbling and gained more weight over the feeding period than cattle fed whole soybeans. Feed conversion was improved in the steers fed the roasted and whole soybean containing diets over steers that consumed the distillers grains diet. Cattle fed soybean meal and distillers grains had similar performance and carcass characteristics in this experiment. Cattle fed soybeans, soybean meal, or distillers had better gains …

Effect Of Using Either Synovex Primer Or Synovex Choice Compared To Not Implanting On The Performance Of Growing Steers, Sawyer D. Starrett, Rebecca L. Mcdermott, Sofia Suarez Lorences, Pedro Henrique Jota Fernandes, Dirk Burken, Jessica L. Sperber, Galen E. Erickson, Jim C. Macdonald

Nebraska Beef Cattle Reports

Summary with Implications

A 120-day winter-growing study utilized 576 head of steers to evaluate three dry lot implant strategies on the growth of steers. The three implanting strategies included a non-implanted control, Synovex® Primer™, or Synovex Choice®. While on harvested corn residue fields, steers were supplemented with 4 lb Sweet Bran and 0.375 lb of a vitamin and mineral package. The targeted gain for the non-implanted control was 1.25 lb/d. Both Synovex Primer and Synovex Choice increased ADG over the non-implanted control cattle by 7.5% and 12.7%, respectively. Additionally, Synovex Choice had a 4.8% increase in ADG over Synovex Primer. …

Evaluation Of Ferappease Administered At Arrival And Time Of Reimplant On Feedlot Performance And Carcass Characteristics Of Yearling Beef Steers, Carsten L. Loseke, Galen E. Erickson, Jessica L. Sperber

Nebraska Beef Cattle Reports

Summary with Implications

Cattle experience stress in the feedlot during handling events such as initial processing and reimplant that can negatively impact morbidity and mortality, feedlot performance, and carcass outcomes. Application of a maternal Bovine Appeasing Substance (mBAS) marketed as FerAppease (FERA Diagnostics and Biologicals) has been hypothesized to mitigate stress in cattle. A commercial feedlot study compared the effectiveness of FerAppease applied in 10mL doses to yearling native beef steers at initial processing and reimplant to a placebo product on cattle performance, morbidity and mortality parameters, and carcass traits. Steers administered FerAppease tended to have improved feed conversion on …

Effect Of Feed Access Time On Feedlot Cattle Performance, Carcass Characteristics, Feeding Behavior And Ruminal Ph, Carsten L. Loseke, Rebecca L. Mcdermott, Josh R. Benton, Keara O'Reilly, Galen E. C. Erickson, Jim C. Macdonald

Nebraska Beef Cattle Reports

Summary with Implications

Bunk management is crucial for modern feedlots, and understanding the ideal time to target a slick bunk is important. A feedlot study compared the effect of providing finishing cattle with 12, 15, 18, 21, or 24 hours of access to feed on performance, carcass characteristics, feeding behavior, and ruminal pH. As access time to feed increased, feed intake and day-to-day variation of feed intake increased linearly, while gain and feed conversion were unchanged. Cattle with greater access time to feed spent more time per day at the bunk, and consumed feed more slowly. A cubic effect on …

Evaluation Of Lysoforte Extend On Rumen Fermentation Parameters, Rumen And Total Tract Digestion, And Fatty Acid Digestion, Anna Kobza, Dalton Anderson, Jerilyn Hergenreder, Jim C. Macdonald

Nebraska Beef Cattle Reports

Summary with Implications

Supplemental fat fed in finishing diets in recent years has generally increased in price. This digestibility study evaluated LYSOFORTE Extend, a lysophospholipid based nutritional emulsifier, in beef finishing diets containing tallow or corn oil. Five ruminally and duodenally cannulated steers were used in a 5 × 5 Latin Square design. Treatments followed a 2 × 2 +1 factorial: 1) No Oil (negative control), 2) Corn Oil (3.5%), 3) Tallow (3.5%), 4) Corn Oil + LFE (8 g/d), and 5) Tallow + LFE (8 g/d). Cattle fed the negative control diet had greater dry matter intake than cattle …

Evaluation Of Renergy On Finishing Steer Growth Performance, Carcass Traits, And Liver Abscess Prevalence And Severity, Sofia Suarez Lorences, Jeff Heldt, Pablo L. Loza, Galen E. Erickson

Nebraska Beef Cattle Reports

Summary with Implications

A feedlot study evaluated the effects of Renergy™ (Selko USA) and Rumensin® (Elanco Animal Health) on growth performance, carcass traits, and liver abscess severity in finishing beef cattle. Using a 2 × 2 factorial design, cattle were fed one of four diets: control (no additive), Renergy, Rumensin, or both additives combined. A significant interaction between Renergy and Rumensin was observed for feed conversion, with a tendency for interaction in dry matter intake (DMI). Cattle fed Renergy tended to have lower DMI and lower average daily gain, though DMI did not differ between cattle fed both additives and …

Evaluation Of Enteric Methane From Cattle Adapted Using Ramp Vs. A Traditional Forage Program Stepped Up To Different Finishing Diets, Jack T. Fort, Isaque F. Vicci, Josh R. Benton, Rebecca L. Mcdermott, Jim C. Macdonald, Maggie E. Youngers, Galen E. Erickson

Nebraska Beef Cattle Reports

Summary with Implications

A 93-day finishing experiment evaluated feeding RAMP (Cargill Corn Milling, Blair NE) during diet adaptation and finishing diet containing 20% Sweet Bran compared to a traditional adaptation program using forage with a finishing diet without Sweet Bran on methane and carbon dioxide production, animal performance and carcass characteristics in beef steers. Cattle were measured using a calorimetry emission barn to quantify the production of methane and carbon dioxide during step 1 of adaptation and 1 time during finishing diet. Feeding RAMP did not significantly reduce methane but did reduce the ratio of methane to carbon dioxide during …

Evaluation Of Enteric Methane From Cattle Adapted To The Same Finishing Diet Using Ramp Versus Forage, Jack T. Fort, Isaque F. Vicci, Josh R. Benton, Rebecca L. Mcdermott, Jim C. Macdonald, Maggie E. Youngers, Galen E. Erickson

Nebraska Beef Cattle Reports

Summary with Implications

A 139-day finishing experiment evaluating the effects of feeding RAMP (Cargill Corn Milling, Blair NE) during diet adaptation compared to a traditional forage program on methane and carbon dioxide emissions was done to corroborate a previous study (2025 Nebraska Beef Report, pp. 55-58). Cattle were measured using a calorimetry emission barn to quantify the production of methane and carbon dioxide during step 1 of adaptation and 1 time during a common finishing diet. Feeding RAMP had no statistical reduction in methane or carbon dioxide production during step 1. When cattle were fed the same finishing …

Health Indicators Worsened By Moderate-Intensity Heat Stress Are Only Partially Recovered By Ω-3 Polyunsaturated Fatty Acid Supplementation, Melanie R. White, Shelley A. Curry, Ashley A. Hahn, Jessica L. Petersen, Ty B. Schmidt, Dustin T. Yates

Nebraska Beef Cattle Reports

Summary with Implications

Heat stress throughout the summer and into the fall is a persistent hurdle for growing and finishing cattle in Nebraska. Minimizing the impact of heat on growth and well-being of cattle is critical to the productivity and profitability of beef production. This project aimed to evaluate the effect of an anti-inflammatory dietary supplement, ω-3 polyunsaturated fatty acids (ω-3 PUFA), on physiological indicators of stress during a 4-day moderate-intensity heat challenge. Heat stress elevated core body temperature and respiration rates as expected but did not impact circulating inflammatory biomarkers. Heart rate variability measurements indicative of adrenergic stress and …

Modulation Of Heat Stress Responses In Cattle Supplemented With Dietary Ω-3 Polyunsaturated Fatty Acids, Shelley A. Curry, Melanie R. White, Ashley A. Hahn, Dustin T. Yates

Nebraska Beef Cattle Reports

Summary with Implications

Heat stress in beef cattle triggers physiological and metabolic disturbances that compromise performance, health, and welfare. In this study, Red Angus heifers exposed to heat stress for 5 days exhibited elevated body temperatures, increased heart and respiration rates, and altered hematological and acid–base parameters. A commercial ω-3 polyunsaturated fatty acid (PUFA) dietary supplement failed to improve heat stress-reduced ribeye area or red blood cell parameters but did resolve the reduction in blood chloride concentrations, which is consistent with restoration of electrolyte balance and hydration under heat stressed conditions. These results indicate that ω-3 PUFA supplementation offers some …

Impact Of Storage And Cooking Temperature On Listeria Innocua Growth In Plant-Based And Beef Proteins, Esther Vanoverbeke, Oliver Munezero, Gary A. Sullivan, Samodha C. Fernando

Nebraska Beef Cattle Reports

Summary with Implications

Listeriosis, caused by Listeria monocytogenes and other Listeria spp., is a food-borne illness, that accounts for 260 deaths annually. The objective of this study was to identify how storage method and cooking temperature impacts Listeria innocua growth in ground beef and plant-based protein patties. Patties (162 ground beef; 162 plant-based) were used in 3 replications. For each replication, patties were either inoculated (1×108 CFU) or not inoculated and stored for 26 hr at refrigeration, freezer, or room temperature. Three patties per storage method were cooked to either 145°F or 165°F and were plated before/after cooking. Nine patties …

Inclusion Of Palm Oil Products In Finishing Diets On Ground Beef Quality And Retail Stability, Anna Kobza, Galen E. Erickson, Jim C. Macdonald, Mitch L. Norman, Josh R. Benton, Rebecca L. Mcdermott, Long Zou, Kyra L. Elliott, Anna G. Hilton, Chad A. Russell, Jessie C. Morrill

Nebraska Beef Cattle Reports

Summary with Implications

Rising costs and availability of supplemental fats have increased interest in alternative fat sources in feedlot finishing diets, including palm oils. This experiment evaluated how refined, bleached, and deodorized (RBD) palm oil products included in the diet of feedlot finishing steers affect beef fat color and ground beef shelf-life. Steers were fed diets with no supplemental fat (No Oil), corn oil (Corn Oil), whole palm (Whole Palm), palm stearin (Stearin), or palm olein (Olein) at 4% dietary DM. Subcutaneous fat from cattle fed corn oil was more yellow than that from No Oil or palm oils. Ground …

Development Of A Shelf-Stable Proxy For Camera-Based Beef Grading Systems, Anna G. Hilton, Jessie C. Morrill

Nebraska Beef Cattle Reports

Summary with Implications

The American Meat Science Association's USDA Grading Committee recently expressed the need for a proxy device to verify that various camera grading systems produce consistent readings. To fulfill this need, we developed a proxy device made from 3D printed epoxy resin and other materials. The proxy was designed to have a known fat thickness and ribeye area. The proxy has a ribeye area of approximately 14 square inches and a fat thickness that ranges from 0.4 - 0.6 inches depending on the exact measurement location. The amount of marbling in the proxy can be adjusted incrementally and …