Life Sciences Commons^™

Hypoxia-Induced Predation Refuge For Northern Quahogs (Mercenaria Mercenaria) In A Temperate Estuary, Bryan P. Galligan S.J., Yoel E. Stuart, M. Conor Mcmanus, Heather E. Stoffel

Biology: Faculty Publications and Other Works

Oxygen depletion in estuaries and coastal waters is often associated with reduced biodiversity, coastal dead zones, and the loss of important ecosystem services. However, some species can benefit from low oxygen conditions due to the indirect effects these conditions have on trophic relationships. In Narragansett Bay, Rhode Island, U.S.A., northern quahogs (Mercenaria mercenaria) reach their highest densities in the areas of the Bay most prone to oxygen depletion. One line of evidence suggests that suboxic events (hypoxia and anoxia) can aid quahogs by excluding predators. Here, we analyze data from long-term surveys of water quality and quahog abundances to test …

Oxidative Stress In Human Pathology And Aging: Molecular Mechanisms And Perspectives, Younis Ahmad Hajam, Raksha Rani, Shahid Yousuf Ganie, Tariq Ahmad Sheikh, Darakhshan Javaid, Syed Sanober Qadri, Sreepoorna Pramodh, Ahmad Alsulimani, Mustfa F. Alkhanani, Steve Harakeh, Arif Hussain, Shafiul Haque, Mohd Salim Reshi

All Works

Reactive oxygen and nitrogen species (RONS) are generated through various endogenous and exogenous processes; however, they are neutralized by enzymatic and non-enzymatic antioxidants. An imbalance between the generation and neutralization of oxidants results in the progression to oxidative stress (OS), which in turn gives rise to various diseases, disorders and aging. The characteristics of aging include the progressive loss of function in tissues and organs. The theory of aging explains that age-related functional losses are due to accumulation of reactive oxygen species (ROS), their subsequent damages and tissue deformities. Moreover, the diseases and disorders caused by OS include cardiovascular diseases …

Performance Determinants Of Unsupervised Clustering Methods For Microbiome Data, Yushu Shi, Liangliang Zhang, Christine B Peterson, Kim-Anh Do, Robert R Jenq

Faculty, Staff and Student Publications

Background: In microbiome data analysis, unsupervised clustering is often used to identify naturally occurring clusters, which can then be assessed for associations with characteristics of interest. In this work, we systematically compared beta diversity and clustering methods commonly used in microbiome analyses. We applied these to four published datasets where highly distinct microbiome profiles could be seen between sample groups, as well a clinical dataset with less clear separation between groups.

Results: Although no single method outperformed the others consistently, we did identify the key scenarios where certain methods can underperform. Specifically, the Bray Curtis (BC) metric resulted in poor …

Green Canopy Cover Percentage As A Method For Quantifying Andropogon Virginicus (Broomsedge) Reduction Through Fertilizer Applications In A Cool Season Hay Production System, Jordyn A. Bush, Jimmy C. Henning, Christopher D. Teutsch

IGC Proceedings (1977-2023)

Remote sensing has been used to measure green canopy cover for a variety of agronomic purposes. This study explores the use of digital imagery as a method to quantify warm and cool season grasses in a hay production system. Due to alternate growth periods, cool and warm season grasses show greener color in different seasons. These seasonal color shifts provide an opportunity to measure their respective percentages when growing together in a system. This study was conducted in a hay field that was originally dominated by cool season grasses including tall fescue (Festuca arundinacea) and Kentucky bluegrass ( …

Global Assessment Of Grassland Carrying Capacities And Relative Stocking Densities Of Livestock, J. Piipponen, M. Jalava, J. De Leeuw, A. Rizayeva, C. Godde, M. Herrero, M. Kummu

IGC Proceedings (1977-2023)

Although many suggest that future diets should include more plant-based proteins, animal-sourced foods are unlikely to completely disappear from our diet. Grasslands yield a notable part of the world’s animal protein production, but thus far, there is no global insight into the relationship between current livestock stocking densities and the availability of grassland forage resources. This inhibits acting upon concerns over the negative effects of overgrazing in some areas and utilising the potential for increasing production in others. Previous research has examined the potential of sustainable grazing but lacks generic and observation-based methods needed to fully understand the opportunities and …

Genetic Diversity Among And Within Accessions Of A Lablab (Lablab Purpureus) Collection Maintained In The Ilri Forage Genebank, M. S. Muktar, A. M. Sartie, A. T. Negawo, E. Habte, C. S. Jones

IGC Proceedings (1977-2023)

Lablab (Lablab purpureus L.) is an important annual multi-purpose legume used as a vegetable for human consumption, as forage for livestock, and as green manure and a cover crop to improve soil fertility. It has a high feed value with good digestibility and high crude protein content. The International Livestock Research Institute (ILRI) forage genebank holds a diverse set of 340 lablab accessions collected from different regions of the world. A total of 1,843 plants from 142 lablab accessions (1 to 29 plants per accession genotyped individually) were genotyped by the genotyping-by-sequencing (GBS) method of the DArTseq platform. The …

Effect Of Very High Frequency Microwave Light On Bread Properties, Dilrabo Tuychieva, Gulnoza Djakhangirova, Yodgorbek Ismoiljonov

CHEMISTRY AND CHEMICAL ENGINEERING

The purpose of the research was to study the effect of microwave on the baking properties of flour obtained from disinfected wheat grain. Trial laboratory baking from grain decontaminated by microwave was carried out. The main physical and chemical indicators of the quality of bakery products are humidity; acidity and porosity. As the object of the study, we used shaped and hearth bread from flour of the 1st grade, made from grain disinfected by microwave. The most acceptable parameters of neutralization were identified, in particular, the duration of exposure to microwave and the amount of power at which the studied …

Investigation Of The Process Of Cotton Cake Extraction Under The Influence Of Pulsed Electric Current, Fazliddin Kayimov, Kakhramon Majidov, Nargiza Sabirova

CHEMISTRY AND CHEMICAL ENGINEERING

The aim of work is study the process of cotton cake extraction by exposure to pulsed electric current. The kinetic equations determined the dependences of technological parameters on external and internal factors. It is established that the technological parameters determine the choice of appropriate dependencies for calculating the parameters of the kinetic equation. Mathematical processing and planning of the experimental data results conducted for extraction of cotton cake.

Activation Of Water Used In The Preparation Of Wheat Grain For Varietal Milling, Suvankul Ravshanov, Ismoil Isabaev

CHEMISTRY AND CHEMICAL ENGINEERING

The purpose of the study is to change the properties of water in order to achieve the optimum impact on wheat grain in preparation for variety milling. The results of influence on the properties of drinking water by acoustic waves in the frequency range of 10-1000 Hz for 1 minute were determined. It is established that the optimal range of exposure is the interval of 80-100 Hz. At the same time water activation is achieved, leading to changes in its properties, such as specific water resistance, electric conductivity, content of dissolved salts, which positively affects the technological indicator - the …

Mise En Visibilité Des Situations D’Exposition Cutanée Aux Pesticides Et Des Pratiques De Prévention : Développement Méthodologique Réalisé En Collaboration Avec Des Producteurs De Pommes Québécois (2e Édition), Caroline Jolly, Sylvie Beaugrand, Ludovic Tuduri, Élise Ledoux, Alain Garrigou

Rapports de recherche scientifique

Dans une optique de rentabilité visant la survie de l’entreprise, et pour répondre aux attentes de qualité du marché, les agriculteurs utilisent des pesticides. La littérature scientifique documente largement les effets à la santé susceptibles d’être liés à l’utilisation des pesticides. Plusieurs auteurs mentionnent l’importance de la voie cutanée comme voie d’exposition aux pesticides. Ce rapport présente le développement d’une méthodologie mixte alliant ergonomie et expologie visant à documenter l’exposition cutanée, les situations d’exposition et les pratiques de prévention associées.

Il s’appuie sur un devis de recherche par étude de cas qui se décline en quatre étapes : une étude …

Interactions Between Strains Govern The Eco-Evolutionary Dynamics Of Microbial Communities, Akshit Goyal, Leonora S. Bittleston, Gabriel E. Leventhal, Lu Lu, Otto X. Cordero

Biology Faculty Publications and Presentations

Genomic data has revealed that genotypic variants of the same species, that is, strains, coexist and are abundant in natural microbial communities. However, it is not clear if strains are ecologically equivalent, and at what characteristic genetic distance they might exhibit distinct interactions and dynamics. Here, we address this problem by tracking 10 taxonomically diverse microbial communities from the pitcher plant Sarracenia purpurea in the laboratory for more than 300 generations. Using metagenomic sequencing, we reconstruct their dynamics over time and across scales, from distant phyla to closely related genotypes. We find that most strains are not ecologically equivalent and …

Nest Records Of Nebraska Birds, Wayne J. Mollhoff

Zea E-Books Collection

Nebraska Ornithologists' Union Occasional Paper Number 9

This publication is an attempt to provide a synopsis of the breeding information accumulated in the past two centuries. As with any compilation like this, other workers would likely come to different conclusions in choosing which records to accept and which to reject. I have tried to state the reasons for my decisions as clearly as possible. Most difficult to categorize are species which are not well documented. Hopefully by laying out the evidence I could find, others will be prompted to do more research, uncover definitive proof, and put more of our …

A Versatile Contribution Of Both Aminopeptidases N And Abc Transporters To Bt Cry1ac Toxicity In The Diamondback Moth, Dan Sun, Liuhong Zhu, Le Guo, Shaoli Wang, Qingjun Wu, Neil Crickmore, Xuguo Zhou, Alejandra Bravo, Mario Soberón, Zhaojiang Guo, Youjun Zhang

Entomology Faculty Publications

BACKGROUND: Biopesticides and transgenic crops based on Bacillus thuringiensis (Bt) toxins are extensively used to control insect pests, but the rapid evolution of insect resistance seriously threatens their effectiveness. Bt resistance is often polygenic and complex. Mutations that confer resistance occur in midgut proteins that act as cell surface receptors for the toxin, and it is thought they facilitate its assembly as a membrane-damaging pore. However, the mechanistic details of the action of Bt toxins remain controversial.

RESULTS: We have examined the contribution of two paralogous ABC transporters and two aminopeptidases N to Bt Cry1Ac toxicity in the diamondback moth, …

Regional Minimums In The U.S. Beef Complex, Elliott James Dennis, Bradley Lubben

Center for Agricultural Profitability

This report shows how the currently proposed policies differ; shows how these policies have aligned with historical market behavior; provides alternative specifications to regional minimums; and suggests policy alternatives to regional minimums.

The main purpose of this report is to show how current and potential alternative specifications of regional minimums would have historically aligned with observed market behavior. However, the fundamental question in the debate of the validity and effectiveness of regional minimums first rests on whether robust price discovery has historically occurred over time and within each USDA-AMS region. If there has been a lack of price discovery during …

Multiple Approaches Converge On Three Biological Subtypes Of Meningioma And Extract New Insights From Published Studies, Tiemo J Klisch, Akash J Patel

Faculty, Staff and Student Publications

One-fifth of meningiomas classified as benign by World Health Organization (WHO) histopathological grading will behave malignantly. To better diagnose these tumors, several groups turned to DNA methylation, whereas we combined RNA-sequencing (RNA-seq) and cytogenetics. Both approaches were more accurate than histopathology in identifying aggressive tumors, but whether they revealed similar tumor types was unclear. We therefore performed unbiased DNA methylation, RNA-seq, and cytogenetic profiling on 110 primary meningiomas WHO grade I and II). Each technique distinguished the same three groups (two benign and one malignant) as our previous molecular classification; integrating these methods into one classifier further improved accuracy. Computational …

Interplay Between Soluble Cd74 And Macrophage-Migration Inhibitory Factor Drives Tumor Growth And Influences Patient Survival In Melanoma, Yasunari Fukuda, Matias A Bustos, Sung-Nam Cho, Jason Roszik, Suyeon Ryu, Jared K Burks, Elizabeth A Grimm, Dave S B Hoon, Suhendan Ekmekcioglu

Faculty, Staff and Student Publications

Soluble forms of receptors play distinctive roles in modulating signal-transduction pathways. Soluble CD74 (sCD74) has been identified in sera of inflammatory diseases and implicated in their pathophysiology; however, few relevant data are available in the context of cancer. Here we assessed the composition and production mechanisms, as well as the clinical significance and biological properties, of sCD74 in melanoma. Serum sCD74 levels were significantly elevated in advanced melanoma patients compared with normal healthy donors, and the high ratio of sCD74 to macrophage-migration inhibitory factor (MIF) conferred significant predictive value for prolonged survival in these patients (p = 0.0035). Secretion of …

Optimizing Management Of Invasions In An Uncertain World Using Dynamic Spatial Models, Kim M. Pepin, Amy J. Davis, Rebecca S. Epanchin-Niell, Andrew M. Gormley, Joslin L. Moore, Timothy J. Smyser, H. Bradley Shaffer, William L. Kendall, Katriona Shea, Michael C. Runge, Sophie Mckee

United States Department of Agriculture Wildlife Services: Staff Publications

Dispersal drives invasion dynamics of nonnative species and pathogens. Applying knowledge of dispersal to optimize the management of invasions can mean the difference between a failed and a successful control program and dramatically improve the return on investment of control efforts. A common approach to identifying optimal management solutions for invasions is to optimize dynamic spatial models that incorporate dispersal. Optimizing these spatial models can be very challenging because the interaction of time, space, and uncertainty rapidly amplifies the number of dimensions being considered. Addressing such problems requires advances in and the integration of techniques from multiple fields, including ecology, …

Evaluation Of Microbial Biomass In Agroforestry Systems Using Forage Cactus And Leucaena Leucocephala And Gliricidia Sepium, Dayanne Camelo, José C. B. Dubeux Jr., Mario De Andrade Lira Jr., Mércia V. F. Dos Santos, Diana Valadares Pessoa, Erick Santos

IGC Proceedings (1977-2023)

Vegetation type can affect soil microbiology in agroforestry systems. Plants release different organic exudates in the rhizosphere, affecting microbial growth. This study evaluated the effect of forage cactus intercropped with different tree legumes on soil microbial biomass. The research was performed in a tropical semiarid Regosol at Caruaru Experimental Station, Agronomic Institute of Pernambuco, Northeast Brazil. Treatments included: i) Gliricidia sepium (Jacq.) Steud and forage cactus IPA-Sertânia (Nopalea cochenillifera Salm Dyck); and ii) Leucaena leucocephala [Lam.] de Wit.) and forage cactus IPA-Sertânia. Treatments were allocated in a randomized complete block design in a split-split-plot scheme, with four replications. Main …

Elephants Mitigate The Effects Of Cattle On Wildlife And Other Ecosystem Traits: Experimental Evidence, Truman P. Young, Duncan N. Kimuyu, Kari E. Veblen, Corinna Riginos, Judith Sitters, Lauren Porensky, Wilfred O. Odadi

IGC Proceedings (1977-2023)

On rangelands worldwide, cattle interact with many ecosystem components, most obviously with soils, plants, and other large herbivores. Since 1995, we have been manipulating the presence of cattle, mesoherbivores, and megaherbivores (elephants and giraffes) in a series of eighteen 4-ha (10-acre) plots at the Kenya Long-term Exclosure Experiment. We have demonstrated a wide array of cattle effects on this savanna rangeland, including their reduction of grass cover, wildlife use, and soil nitrogen and phosphorus pools, but their increase of primary productivity and termite abundance. Strikingly, we demonstrate that the presence of mega-herbivores (elephants, mainly) reduces the sizes of these cattle. …

Practice Cost And Size Differences In Invasive Plant Management Strategies: An Empirical Analysis Of Us Great Plains States, Mazbahul G. Ahamad

Department of Agronomy and Horticulture: Faculty Publications

Grassland conservation of the Conservation Stewardship Program in the United States (US) is one of the largest cost-sharing initiatives for protecting grazing land from invasive and woody plants. The practice cost and unit size of various invasive and woody plant management strategies, such as mulching, brush management, and pre- scribed burning, are different from state to state. We aimed to compare and examine the association between practice cost ($/acre[ac]) and standard unit size of practice (acre) of mulching, brush management, and pre- scribed burning strategies in nine US Great Plains states, including Kansas, Montana, Nebraska, New Mexico, North Dakota, Oklahoma, …

A Highly Selective And Sensitive Spectrofluorimetric Method For The Assessment Of 3-Nitrotyrosine In Serum Using (Eu(Tta)3phen) Photo Probe, Ekram Hany Mohamed, Mohamed S. S. Attia, Ahmed O. Youssef, Nour El-Din A. Abdel-Sattar, Mohammed A. Amin,, Sarah Alharthi, Safwat A. Mahmoud,, Mona N. Abou-Omar,

Pharmacy

A simple, accurate and fast method was developed for the assessment of 3-nitrotyrosine as a biomarker for the early diagnosis of liver cirrhosis with minimal hepatic encephalopathy (MHE) using a (Eu(TTA)3Phen) photo probe. 3-Nitrotyrosine can remarkably quench the luminescence intensity of the (Eu(TTA)3Phen) complex in DMSO at pH = 9 and λem = 617 nm. The quenching of the luminescence intensity of (Eu(TTA)3Phen) complex particularly the electrical emission band at λem = 617 nm is used for the assessment of 3-nitrotyrosine in different serum samples of patients with liver cirrhosis

Disentangling The Role Of Religiosity In Human Papillomavirus Vaccination Amidst Covid-19 Pandemic, Ayokunle Olagoke, Brenikki Floyd, Rachel Caskey, Jennifer Hebert-Beirne, Andrew D. Boyd, Yamilé Molina

Health and Kinesiology Faculty Publications

Religion is a complex and sociocultural driver of human papillomavirus (HPV) vaccination decisions, but its exact role has been mixed/unclear. We used a cross-sectional study of 342 Christian parents to examine the associations between the three domains of religiosity (organizational, non-organizational, and intrinsic) and the intention to (i) seek HPV information and (ii) receive the HPV vaccine. Organizational religiosity was the only domain that was positively associated with information-seeking intention regardless of the type of covariates included. Mixed findings in the association between religiosity and HPV vaccination decisions may depend on the religiosity domain being assessed.

Apoe Genetics Influence Murine Gut Microbiome, Diana J. Zajac, Stefan J. Green, Lance A. Johnson, Steven Estus

Physiology Faculty Publications

Apolipoprotein E (APOE) alleles impact pathogenesis and risk for multiple human diseases, making them primary targets for disease treatment and prevention. Previously, we and others reported an association between APOE alleles and the gut microbiome. Here, we evaluated effects of APOE heterozygosity and tested whether these overall results extended to mice maintained under ideal conditions for microbiome analyses. To model human APOE alleles, this study used APOE targeted replacement (TR) mice on a C57Bl/6 background. To minimize genetic drift, homozygous APOE3 mice were crossed to homozygous APOE2 or homozygous APOE4 mice prior to the study, and the resulting …

Mapk-Mediated Transcription Factor Gatad Contributes To Cry1ac Resistance In Diamondback Moth By Reducing Pxmalp Expression, Le Guo, Zhouqiang Cheng, Jianying Qin, Dan Sun, Shaoli Wang, Qingjun Wu, Neil Crickmore, Xuguo Zhou, Alejandra Bravo, Mario Soberón, Zhaojiang Guo, Youjun Zhang

Entomology Faculty Publications

The benefits of biopesticides and transgenic crops based on the insecticidal Cry-toxins from Bacillus thuringiensis (Bt) are considerably threatened by insect resistance evolution, thus, deciphering the molecular mechanisms underlying insect resistance to Bt products is of great significance to their sustainable utilization. Previously, we have demonstrated that the down-regulation of PxmALP in a strain of Plutella xylostella (L.) highly resistant to the Bt Cry1Ac toxin was due to a hormone-activated MAPK signaling pathway and contributed to the resistance phenotype. However, the underlying transcriptional regulatory mechanism remains enigmatic. Here, we report that the PxGATAd transcription factor (TF) is responsible for the …

New Opportunities And Untapped Scientific Potential In The Abyssal Ocean, Jeffrey J. Marlowe, Rika E. Anderson, Anna-Louise Reysenbach, Jeffrey S. Seewald, Timothy M. Shank, Andreas P. Teske, V. Dorsey Wanless, S. Adam Soule

Biology Faculty Publications and Presentations

The abyssal ocean covers more than half of the Earth’s surface, yet remains understudied and underappreciated. In this Perspectives article, we mark the occasion of the Deep Submergence Vehicle Alvin’s increased depth range (from 4500 to 6500 m) to highlight the scientific potential of the abyssal seafloor. From a geologic perspective, ultra-slow spreading mid-ocean ridges, Petit Spot volcanism, transform faults, and subduction zones put the full life cycle of oceanic crust on display in the abyss, revealing constructive and destructive forces over wide ranges in time and space. Geochemically, the abyssal pressure regime influences the solubility of constituents such as …

Charcoal Value Chain As A Complimentary Livelihood Activity For Pastoralists In The Rangelands, S. Teyie, Oliver Vivian Wasonga, C. Bergmann, M. Petersen

IGC Proceedings (1977-2023)

Global charcoal production has more than tripled over the past 50 years from 17.3 million tons in 1964 to 53.1 million tons in 2014. 61% of the present global charcoal production occurs in Africa, mostly to satisfy demand for cooking fuel from urban and peri-urban households. Despite the adverse environmental impacts associated with charcoal production, a significant global population relies on it for energy needs, while many producers depend on it as a livelihood source. Whereas this calls for efforts to promote sustainable charcoal production practices, this has to be informed by in-depth understanding of the charcoal value chain to …

Nutritive Evaluation Of Mulberry Leaves Based Apple Pomace Silage, Ngahanyui Kengoo, Rohit Bishist

IGC Proceedings (1977-2023)

The present study was conducted to evaluate the nutritive value of mulberry leaves based apple pomace silage in Dr Y S Parmar University of Horticulture and Forestry, Nauni, Solan, Himachal Pradesh, India. In the study, the silage was prepared by mixing of maize, apple pomace (AP) and mulberry leaves in ratios of 80:10:10 (T1), 70:20:10(T2) and 60:30:10 (T3), and mixing of apple pomace with wheat straw in the ratio of 85:15(T4), and the silage prepared by using 100 per cent chopped maize was taken as control. Silage bags were opened after a period of three months and the proportionate samples …

Dairy Cattle Genetics By Environment Interaction Mismatch Contributes To Poor Mitigation And Adaptation Of Grazing Systems To Climate Change Actions In The Peruvian High Andes: A Review, V. M. Velez Marroquin, J. Manhire, R. Garcia Ticllacuri, J. L. Bernal Madrid, C. S. Pinares-Patino

IGC Proceedings (1977-2023)

The high Andes of Peru includes fragile ecosystems. Nevertheless, it plays important ecosystem functions (e.g., biodiversity, water supply for the lowlands, CO₂ sinks in soil, etc). More than 80% of the livestock population of Peru is farmed in this area, supporting the livelihood of approximately 1’400,000 poor families, who are vulnerable to climate change (CC). Climate change in the high Andes is occurring at accelerated rates, compared to lowlands regions. Prevalent factors in the high Andes, such as hypoxia, high UV radiation, climatic extremes, large variation between maximum and minimum temperatures, seasonality in rainfall (determining highly seasonal forage growth) …

Exploring The Variation In Spatial Landscape Utilisation By Cows In The Pre- And Post-Partum Period Using Gnss Technology, A Z. Chang, M. G. Trotter

IGC Proceedings (1977-2023)

On-animal sensors refer to systems that monitor the location, behaviour, or physiological characteristics of livestock. These technologies provide a method to overcome many of the challenges that are associated with monitoring livestock within an extensive grazing system. A global navigation satellite system (GNSS) could be used to detect the changes in spatial utilisation associated with when a cow has a calf at foot. In this study, forty pregnant Belmont Red heifers were fitted with GNSS collars programmed to provide a location reading at 5 minute intervals. The data was cleaned and mapped in ArcGIS to identify changes in paddock area …

Sustainable Intensification Of Livestock Systems Using Forage Legumes, José C. B. Dubeux Jr., Lynn E. Sollenberger

IGC Proceedings (1977-2023)

Global human population is increasing and expected to reach 9.7 billion people by 2050. Sustainable intensification (SI) of agricultural systems is key to increase food production while minimizing impact on global natural resources. Forage legumes provide a myriad of ecosystem services (ES) and represent an important tool for promoting SI in livestock systems. Forage legumes associate with soil microorganisms to reduce atmospheric N₂. This N input represents a valuable contribution to increase net primary productivity with reduced C footprint. In addition, forage nutritive value generally increases, resulting in greater animal performance. When forage legumes are integrated into livestock …