Life Sciences Commons^™

Single-Cell Transcriptome And Network Analyses Unveil Key Transcription Factors Regulating Mesophyll Cell Development In Maize, Shentong Tao, Peng Liu, Yining Shi, Yilong Feng, Jingjing Gao, Lifen Chen, Hairong Wei, Et, Al,

Michigan Tech Publications, Part 1

BACKGROUND: Maize mesophyll (M) cells play important roles in various biological processes such as photosynthesis II and secondary metabolism. Functional differentiation occurs during M-cell development, but the underlying mechanisms for regulating M-cell development are largely unknown. RESULTS: We conducted single-cell RNA sequencing (scRNA-seq) to profile transcripts in maize leaves. We then identified coregulated modules by analyzing the resulting pseudo-time-series data through gene regulatory network analyses. , , , and () families were highly expressed in the early stage, whereas () and families were highly expressed in the late stage of M-cell development. Construction of regulatory networks revealed that these transcript …

Exploring Pathways From Data To Knowledge To Insights In The Pharmaceutical Industry: ‘Introducing The Pharmaceutical Knowledge Ecosystem’, Melanie J. Adams, Paige E. Kane, Anne Greene (Editor), Martin Lipa

Level 3

The ecosystem of how the pharmaceutical industry acquires data, transforms these data into tangible knowledge, and derives valuable insights throughout the process, is highly complex. Data, information, knowledge, and the resulting insights, are necessary to support decision- making, manage risk, problem solve, ensure product realisation, enable continual improvement, and enhance operational effectiveness. Building on the fundamental concepts established in the well-known Data Information Knowledge Wisdom (DIKW) hierarchy, this paper reviews the basic concepts involved in the DIKW pathway and begins to relate these concepts to both established capabilities (e.g., PAT), existing requirements (e.g., data integrity), and emerging trends in the …

Improving Risk-Based Decision Making (Rbdm) Effectiveness Through A Framework Which Integrates Knowledge And Risk, Martin Lipa, Valerie Mulholland, Anne Greene (Editor)

Level 3

This paper explores the emergence of the importance of knowledge in Risk-Based Decision Making (RBDM) and Quality Risk Management (QRM) through a detailed review of ICH Q9(R1) (Step 2) and presents a framework(the RKI cycle) to integrate knowledge and risk and associated six principles to operationalise it.

Rare Coding Variants In Rcn3 Are Associated With Blood Pressure, Karen Y. He, Tanika N. Kelly, Heming Wang, Jingjing Liang, Luke Zhu, Brian E. Cade, Themistocles L. Assimes, Lewis C. Becker, Amber L. Beitelshees, Lawrence F. Bielak, Adam P. Bress, Jennifer A. Brody, Yen-Pei Christy Chang, Yi-Cheng Chang, Paul S. De Vries, Ravindranath Duggirala, Ervin R. Fox, Nora Franceschini, Anna L. Furniss, Yan Gao, Donna K. Arnett

Epidemiology and Environmental Health Faculty Publications

BACKGROUND: While large genome-wide association studies have identified nearly one thousand loci associated with variation in blood pressure, rare variant identification is still a challenge. In family-based cohorts, genome-wide linkage scans have been successful in identifying rare genetic variants for blood pressure. This study aims to identify low frequency and rare genetic variants within previously reported linkage regions on chromosomes 1 and 19 in African American families from the Trans-Omics for Precision Medicine (TOPMed) program. Genetic association analyses weighted by linkage evidence were completed with whole genome sequencing data within and across TOPMed ancestral groups consisting of 60,388 individuals of …

Vaccinia Virus Arrests And Shifts The Cell Cycle, Caroline K. Martin, Jerzy Samolej, Annabel T. Olson, Cosetta Bertoli, Matthew S. Wiebe, Robertus A. M. De Bruin, Jason Mercer

School of Veterinary and Biomedical Sciences: Faculty Publications

Modulation of the host cell cycle is a common strategy used by viruses to create a proreplicative environment. To facilitate viral genome replication, vaccinia virus (VACV) has been reported to alter cell cycle regulation and trigger the host cell DNA damage response. However, the cellular factors and viral effectors that mediate these changes remain unknown. Here, we set out to investigate the effect of VACV infection on cell proliferation and host cell cycle progression. Using a subset of VACV mutants, we characterise the stage of infection required for inhibition of cell proliferation and define the viral effectors required to dysregulate …

The Role Of Improved Forages In Solving The Water Scarcity Issue Of 4 Billion People, S. Douxchamps, A. Notenbaert, J. A. Cardoso, M. Romero, M. Peters

IGC Proceedings (1977-2023)

Global animal production requires about 2422 Gm³ of water per year. Most of this volume is used by cattle, with 30% used in the beef sector and another 19% in the dairy sector. At the same time, four billion people suffer from severe water scarcity, raising the flag on diverting an important part of the water globally to livestock production. Livestock-water interactions have therefore gained a prominent profile and fuelled discussions on the water footprint of livestock. A series of studies on the most important components of the livestock water footprint have shown that globally more than 90% of …

Spring Forage Stash Module To Prevent Forage Crisis On Uruguayan Livestock Systems: An Evaluation Based On Model Simulations, M. Pereira Machín, F. Dieguez Cameroni

IGC Proceedings (1977-2023)

In Uruguay, rangeland cattle production systems support national economy by beef exportation chain and economic inputs to the country. Soil hydric stress episodes results to forage crisis on grassland-based production systems, having high impact on its trajectory and leaving sequels at several farm levels mainly on rearing cattle systems in drought sensitive regions of the country. In that context, the proposal is to create a spring forage stash module, with a simple management rules as a mechanism to build stability to farms and to buffering drought impact. The concept is to differ spring forage (season with low variation coefficient on …

Species Richness Increased Yield Stability In Intensively Managed Grasslands Subjected To Experimental Drought, J. A. Finn, M. Suter, E. Haughey, D. Hofer, A. Lüscher

IGC Proceedings (1977-2023)

Climate change is expected to cause an increase in the frequency and intensity of drought events. Over two years we investigated the effects of experimentally imposed drought on intensively managed grassland communities (5 m x 6 m plots) of varying richness (1, 2 and 4 species), and comprising four species (Lolium perenne L., Cichorium intybus L., Trifolium repens L., Trifolium pratense L.). In each year a summer drought period of nine weeks with complete exclusion of precipitation was simulated, inducing severe drought stress at Reckenholz (Zürich, Switzerland), and extreme drought stress at Wexford (Ireland). Mean yield and plot-to-plot variance …

Soil Nitrous Oxide (N2O) Emission From Integrated Soil Fertility Management In Maize (Zea Mays L.) Cropping Systems, R. Rogito, N. Karanja, L. Merbold, M. Necpalova, J. Six

IGC Proceedings (1977-2023)

Integrated Soil Fertility Management (ISFM) has been recommended to address challenges of low soil fertility by incorporating locally available organic resources (ORs) together with inorganic nitrogen (N) fertilizers. Despite ISFM success in field trials, there is limited information on ORs contribution to atmospheric greenhouse gas concentrations through N₂O emission. A short-term field study was conducted at two sites with different soil types; silt loam (Aludeka) and silty-clay soil (Sidada) to assess the influence of selected ORs on soil N₂O emissions. The ORs treatments included; Calliandra carothyrsus (CL), farmyard manure (FYM) and maize stover (MS) with (+N) …

A Facial Congenital Anomaly In A Mature Male White-Tailed Deer (Odocoileus Virginianus), Travis Cunningham, Al Mead

Georgia Journal of Science

Congenital anomalies are rarely documented in wild ungulates. This study describes a congenital facial malformation in a mature male white-tailed deer harvested in central Georgia in 2020. The skull displays a mediolateral deflection of the rostrum, and the mandibles display similar deflection with posterior rotation at the mandibular symphysis, a maxillofacial malformation commonly called wry face. Based on physical examination and radiographic imagery, there were no signs of neoplasia or healed bone trauma on the skull or jaws, suggesting a congenital origin for the deformity. Studies of domestic horses displaying wry face conclude that the malformation arises from fetal mispositioning …

Attitudes Toward The Environment: How Do The Attitudes Of Conventional, No-Till, And Organic Farmers Compare?, George A. Youngs, David L. Watt

Great Plains Sociologist

One of Che key dynamics in today's increased interest in alternative fanning is concern for the environment (Beus and Dunlap, 1990). Many advocates of alternative farming argue that conventional farming harms the environment and may even destroy the future of agriculture. The implicit, and often explicit, notion associated with this view is that conventional farmers are less concerned about the environment than are alternative farmers. The present study will test this notion by comparing the attitudes toward the environment of conventional farmers with two types of alternative farmers, organic and no-till. By definition, conventional farmers are those farmers who practice …

Response Of Soil Respiration To Precipitation Variation In Alfalfa Grassland On The Western Loess Plateau: Hysteresis And Diel Q10, H. K. Chu, H. Ni, J. Y. Ma, Yuying Shen

IGC Proceedings (1977-2023)

Soil respiration (Rs) plays an important role in the terrestrial carbon cycle, but how precipitation variation affects Rs in alfalfa grassland is poorly understood. To explore the effects of precipitation variation on Rs, this experiment was conducted by using rainfall shelters to simulate 30% precipitation reduction (P-30), normal precipitation (CK) and 30% precipitation increase (P+30) for an alfalfa (Medicago sativa) grassland in the Qingyang city, Gansu province, China. Rs was measured continuously using an automated soil respiration system (model LI-8100A with an LI-8150 multiplexer, LI-COR, Nebraska, USA) during the overwintering preparation period of alfalfa. The results showed that …

Soil Carbon Accumulation Under Perennial Forage Grasses In The Southern Highlands Of Tanzania, S. S. Nyawira, P. Birthe, M. J. Korir, B. Nzogela, A. O. Notenbaert

IGC Proceedings (1977-2023)

Land degradation caused by the loss of SOC in continuously cultivated agricultural systems is a major problem in many sub-Saharan Africa countries. The integration on perennial forage grasses in cropping systems has the potential to enhance SOC sequestration. The main objective of this study is to compare soil organic carbon (SOC) under perennial forages with SOC under annual food crops, specifically maize (Zea mays). A survey was conducted in Njombe district in the Southern Highlands of Tanzania to identity farmers with planted forages that are more than five years old and with neighbouring maize plots. Survey results identified …

Perennial Forage Grasses Response To Deficit Irrigation As An Alternative For Water-Limited Conditions Of Southern Chile, Rafael López-Olivari, Fernando Ortega-Klose

IGC Proceedings (1977-2023)

Perennial grasses are an important pasture forage source for the animal production industry around the world. However, the lack of available water limits the use and efficiency of the water resource for maximizing the forage yield. Furthermore, the highest perennial grasses production is obtained in southern Chile, where the frequency and intensity of the rainfalls has been significantly diminished in the main growing period. Thus, the purpose of this research was to evaluate the responses of forage yield (FY) and irrigation water use efficiency (I_WUE) under different levels of water stress on perennial grasses sown …

Quantification Of Methane Emissions From Indoor-Fed Fogera Dairy Cows Using Laser Methane Detector, N. Kobayashi, F. Hou, A. Tsunekawa, Tianhai Yan, F. Tegegne, A. Tassew, Y. Mekuriaw, S. Mekuriaw, B. Hunegnaw, W. Mekonnen, T. Ichinohe

IGC Proceedings (1977-2023)

Portable laser methane detectors (LMDs) may be an economical means of estimating CH₄ emissions from ruminants. Here, we validated an LMD-based approach and then used that approach to evaluate CH₄ emissions from indigenous dairy cows in a dryland area of Ethiopia. First, we validated our LMD-based approach in Simmental crossbred beef cattle (n = 2) housed in respiration chambers and fed either a high- or low-concentrate diet. We found that the exhaled air CH₄ concentrations measured by LMD were linearly correlated with the CH₄ emissions determined by infrared-absorption-based gas analyzer (r² = 0.55). …

P53-Mediated Neurodegeneration In The Absence Of The Nuclear Protein Akirin2., Stacey L Peek, Peter J Bosch, Ethan Bahl, Brianna J Iverson, Mrutyunjaya Parida, Preeti Bais, J Robert Manak, Jacob J Michaelson, Robert W. Burgess, Joshua A Weiner

Faculty Research 2022

Proper gene regulation is critical for both neuronal development and maintenance as the brain matures. We previously demonstrated that Akirin2, an essential nuclear protein that interacts with transcription factors and chromatin remodeling complexes, is required for the embryonic formation of the cerebral cortex. Here we show that Akirin2 plays a mechanistically distinct role in maintaining healthy neurons during cortical maturation. Restricting Akirin2 loss to excitatory cortical neurons resulted in progressive neurodegeneration via necroptosis and severe cortical atrophy with age. Comparing transcriptomes from Akirin2-null postnatal neurons and cortical progenitors revealed that targets of the tumor suppressor p53, a regulator of both …

Mobility Of Phosphine-Susceptible And -Resistant Rhyzopertha Dominica (Coleoptera: Bostrichidae) And Tribolium Castaneum (Coleoptera: Tenebrionidae) After Exposure To Controlled Release Materials With Existing And Novel Active Ingredients, Sabita Ranabhat, Kun Yan Zhu, Georgina Bingham, William R., Morrison Iii

Department of Entomology: Faculty Publications

There is interest in developing controlled release materials (CRMs) with novel modes of action to improve resistance management. Long-lasting insecticide-incorporated netting (LLIN) with deltamethrin has been effectively used against stored-product pests. Here, we evaluated the efficacy of different CRMs (LLIN or packaging) with each of four active ingredients (AI) (deltamethrin, permethrin, indoxacarb, and dinotefuran) and compared them to control CRMs in reducing movement and increasing mortality of phosphine-susceptible and -resistant Rhyzopertha dominica and Tribolium castaneum. Adults were exposed for 0.5, 2, or 60 min, and movement was assessed immediately or after 24, or 168 h using video-tracking and Ethovision …

Reducing Adverse Impacts Of Amazon Hydropower Expansion, Alexander S. Flecker, Qinru Shi, Rafael M. Almeida, Héctor Angarita, Jonathan M. Gomes-Selman, Roosevelt García-Villacorta, Suresh A. Sethi, Steven A. Thomas, N Leroy Poff, Bruce R. Forsberg

School of Earth, Environmental, & Marine Sciences Faculty Publications

Proposed hydropower dams at more than 350 sites throughout the Amazon require strategic evaluation of trade-offs between the numerous ecosystem services provided by Earth's largest and most biodiverse river basin. These services are spatially variable, hence collective impacts of newly built dams depend strongly on their configuration. We use multiobjective optimization to identify portfolios of sites that simultaneously minimize impacts on river flow, river connectivity, sediment transport, fish diversity, and greenhouse gas emissions while achieving energy production goals. We find that uncoordinated, dam-by-dam hydropower expansion has resulted in forgone ecosystem service benefits. Minimizing further damage from hydropower development requires considering …

Adrenergic Modulation Of Precursor Cells Of Ovarian Cancer, Sweta Dash

USF Tampa Graduate Theses and Dissertations

Ovarian cancer remains one of the most lethal gynecological cancer and ranks eighth in cancer-related mortality among women. The high mortality rate can be attributed to majority of the cases being diagnosed at advanced stages of the disease when the survival rate and overall prognosis are poor. There is lack of effective screening modalities to detect ovarian cancer at early stages. Hence, a better understanding of the molecular mechanisms leading to ovarian cancer initiation is required.

Epidemiological studies have reported that conditions which cause chronic behavioral stress are associated with a higher risk of developing ovarian cancer. The activation of …

Smokejumper Obituary: Palmer, Christopher Richard “Chris” (Redding 1975), National Smokejumper Association

Smokejumper Obituaries

No abstract provided.

Amorphization Of Pseudocapacitive T−Nb2O5 Accelerates Lithium Diffusivity As Revealed Using Tunable Isomorphic Architectures, Wessel Van Den Bergh, Sean Wechsler, Hasala Nadeesini Lokupitiya, Lauren Jarocha, Kwangnam Kim, James Chapman, Kyoung E. Kweon, Brandon C. Wood, Steve Heald, Morgan Stefik Phd

Faculty Publications

Intercalationpseudocapacitancecan combinecapacitor-likepower densitieswith battery-likeenergy densities.Such surface-limitedbehaviorrequiresrapid diffusionwhere amorphizationcan increasesolid-statediffusivity.Here intercalationpseudoca-pacitivematerialswith tailoredextentsof amorphizationin T-Nb2O5are first reported.Amorphizationwas characterizedwithWAXS, XPS, XAFS, and EPR which suggesteda peroxide-rich(O22) surface that was consistentwith DFT predictions.A seriesof tunableisomorphicarchitecturesenabledcomparisonswhileindependentlyvaryingtransportparameters.Throughprocessof elimination,solid-statelithium diffusionwas identifiedas thedominantdiffusive-constraintdictatingthe maximumvoltagesweep rate for surface-limitedkinetics(vSLT), termed the Surface-LimitedThreshold(SLT). ThevSLTincreasedwith amorphizationhoweverstable cycling requiredcrystallineT-Nb2O5. A current-responsemodel using series-impedanceswell-matchedtheseobservations.This perspectiverevealedthat amorphizationof T-Nb2O5enhancedsolid-statediffusionby 12.2% and increasedsurface-limitationsby 17.0% (stablesamples).This approachenabledretaining95% lithiationcapacityat ~800mVs1(1,600C-rate equivalent).

Tissue Losses And Metabolic Adaptations Both Contribute To The Reduction In Resting Metabolic Rate Following Weight Loss, Alexandra Martin, Darius Fox, Chaise A. Murphy, Hande Hofmann, Karsten Koehler

Department of Nutrition and Health Sciences: Faculty Publications

OBJECTIVE: To characterize the contributions of the loss of energy-expending tissues and metabolic adaptations to the reduction in resting metabolic rate (RMR) following weight loss.

METHODS: A secondary analysis was conducted on data from the Comprehensive Assessment of Long-term Effects of Reducing Intake of Energy study. Changes in RMR, body composition, and metabolic hormones were examined over 12 months of calorie restriction in 109 individuals. The contribution of tissue losses to the decline in RMR was determined by weighing changes in the size of energy-expending tissues and organs (skeletal muscle, adipose tissue, bone, brain, inner organs, residual mass) …

A Review Of The Impacts Of Invasive Wild Pigs On Native Vertebrates, Matthew T. Mcdonough, Stephen S. Ditchkoff, Mark D. Smith, Kurt C. Vercauteren

United States Department of Agriculture Wildlife Services: Staff Publications

The wild pig (Sus scrofa) is a successful invasive species that has become well established outside of its native range in Eurasia. The invasive wild pig is the result of released or escaped domesticated livestock becoming feral, or Eurasian boar introduced for hunting purposes. The global spread of wild pigs has recently been exacerbated in some areas, such as the USA, by anthropogenically assisted dispersal. Once established in novel ecosystems, wild pigs have the potential to have significant negative impacts on the ecosystem, and the scientific literature is replete with examples. It is generally accepted that wild pigs …

Seizure Prediction In Epilepsy Patients, Gary Dean Cravens

NSU REACH and IPE Day

Purpose/Objective: Characterize rigorously the preictal period in epilepsy patients to improve the development of seizure prediction techniques. Background/Rationale: 30% of epilepsy patients are not well-controlled on medications and would benefit immensely from reliable seizure prediction. Methods/Methodology: Computational model consisting of in-silico Hodgkin-Huxley neurons arranged in a small-world topology using the Watts-Strogatz algorithm is used to generate synthetic electrocorticographic (ECoG) signals. ECoG data from 18 epilepsy patients is used to validate the model. Unsupervised machine learning is used with both patient and synthetic data to identify potential electrophysiologic biomarkers of the preictal period. Results/Findings: The model has shown states corresponding to …

A Systematic Review To Propose An Evidence-Based Nutrition Protocol In Depression, Camilla A. Mirkin, Nicole Koci

NSU REACH and IPE Day

Background: Rates of depression have been rising steadily over the last several decades with more than 264 million people affected, making it one of the top three burdens globally. The pandemic has brought on a greater risk of depression. Forty-two percent of individuals surveyed in December 2020 by the US Census Bureau reported depressive symptoms. Many factors, including poor diet quality, play a role in the development of depression during traumatic events like the pandemic. A low-quality dietary pattern has been associated with obesity, low-grade chronic inflammation, dysbiosis, increased cortisol levels and monoamine neurotransmitter deficiency. Among others, these factors have …

An Ecophisiological Proposal To Manage Natural Grasslands: A Long Term Trial, F. L. F. De Quadros, D. Roberti, L. Marin, B. Kuinchtner, P. L. Nascimento

IGC Proceedings (1977-2023)

Natural grasslands on Southern Brazil comprise the so called “Rio de La Plata Grasslands” in South America. They are an important fodder source for ruminant pastoral systems and contribute to regional ecosystem services. Strength of these grasslands is its floristic diversity that poses a dilemma to farmers: how to choose management protocols that could be applied for hundreds of species. We propose to use a functional ecophysiological approach based on groups of grasses, the most abundant on aerial biomass of this natural grasslands. We clustered the most frequent grasses in two groups based on its leaf traits (leaf dry matter …

When The Natural Toxin Concentration In Crotalaria Increase In Japan?, M. Kaneko, N. Kato, I. Hattori

IGC Proceedings (1977-2023)

A green manure crop, sunn hemp (Crotalaria juncea L.), legumes, has a nematode-suppressive effect. It is also used as feed in India. However, it is not used as feed in Japan because it contains natural toxins: Pyrrolizidine alkaloids (PAs). PAs has the negative effects for not only livestock but also human health. The PAs, which are abundant in sunn hemp seeds and which are contained only slightly in leaves and stems. Therefore, if sunn hemp harvested before flowering, then no poison problem is posed by PAs. However, if it is harvested at the podding stage, a poisonous concentration of …

Accelerate Scaling Up Forage Intensification Using Novel Digital Extension Approach In Kenya, Donald M. G. Njarui, Mwangi Gatheru, Jessica M. Ndubi, Alice W. Murage, Anne W. Gichangi, Elias M. Gichangi, Udaya S. Nogothu

IGC Proceedings (1977-2023)

Wide scale adoption of diverse forages improves livestock productivity and farmers welfare. However, limited access to information and knowledge on forage production results in slow adoption in Kenya. There is need to enhance information and knowledge exchange among farming communities for efficient and effective adoption and decision-making. An inter-institutional pilot project was initiated in 2017 to scale-up forages in Kenya using a novel extension approach - the village knowledge centre (VKC). A VKC is an information and communication technology (ICT) digital platform-based linking farmers through smart phones and social media as a conduit for faster and effective information and knowledge. …

Evaluation Of Annual Companion Crops For The Establishment Of Perennial Forage Crops In Eastern Canada, Philippe Seguin, Sandrine St-Pierre-Lepage, Céline Georlette, Caroline Halde, Gaëtan F. Tremblay, Huguette Martel, Ayitre Akpakouma

IGC Proceedings (1977-2023)

The use of companion crops when establishing perennial forages is desirable as it often reduces weed growth and increases forage biomass in the seeding year. In eastern Canada, oat (Avena sativa L.) is the main species used as companion crop; although other species are used, they have not been systematically evaluated. A field study was established in 2019 at three sites in Québec, Canada, to contrast the use of six annual species as companion crops for the establishment of lucerne (Medicago sativa L.)-timothy (Phleum pratense L.) mixtures. Species evaluated include berseem clover (Trifolium alexandrinum L.), annual …

Variation Of Pollen Viability In Brachiaria Species And Opportunity In Increased Filled Seed Percentage, Weenaporn Juntasin, Sarayut Thaikua, Rattikan Poungkaew, Pichet Chanpeng, Sompon Waipanya, Yasuhiro Kawamoto

IGC Proceedings (1977-2023)

On pseudogamy process, pollen fertility affects endosperm development in Brachiaria apomictic hybrids. Also, it was well-known that self-incompatibility could affect the fertilization in cross pollination species. Evaluation of pollen viability and percentage of filled seed, and the demonstration of pollen induction technique for increasing filled seed percentage in Brachiaria spp. are presented in this paper. In the first experiment, 8 cultivars/breeding lines of Brachiaria, including 2 open-pollinated cultivars, an apomictic cultivar and 5 hybrids, were planted in randomized completely block design, with 3 replications at northeast Thailand. Each experimental unit consisted of 20 plants, 6 central plants were evaluated. …