Life Sciences Commons^™

Greater Numbers Of Antral Follicles In The Ovary Are Associated With Increased Concentrations Of Glucose In Uterine Luminal Fluid Of Beef Heifers, Alexandria P. Snider, Matthew S. Crouse, Shelby L. Rosasco, Kaitlin M. Epperson, Emmalee J. Northrop-Albrecht, Jerica J.J. Rich, Chadwick C. Chase Jr., Jeremy R. Miles, George A. Perry, Adam F. Summers, Robert A. Cushman

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Increased antral follicles are associated with greater fertility and a uterine environment that is more supportive of early embryonic development in beef heifers. Glucose is a primary energy source for embryos, and glucose concentrations are elevated in uterine luminal fluid (ULF) of pregnant heifers. We hypothesized that ULF glucose concentrations and endometrial transcript abundance for glucose transporters on d16 after insemination would be greater in heifers with increased numbers of antral follicles. Heifers classified with either increased or diminished antral follicle counts were artificially inseminated following the CO-Synch protocol (d0). On d16 after insemination, reproductive tracts of heifers were collected …

Smokejumper Obituary: Stillman, Albert Theodore “Al” (Mccall 1959), National Smokejumper Association

Smokejumper Obituaries

No abstract provided.

School Of Culinary Arts And Food Technology Newsletter-Spring Edition 2022, James Murphy

Other resources

The School of Culinary Arts and Food Technology, TU Dublin, Spring Newsletter captured the many events, research, awards, significant contributions and special civic and community activities which the students and staff members of the school have successfully completed up to the Spring period of 2022. The successful completion of these activities would not be possible without the active and on-going support of the 'INSPIRED' friends of Culinary Arts (school supporters) and our school's industry association supporters.

Reduction Of Stabilin-2 Contributes To A Protection Against Atherosclerosis, Yukako Kayashima, Connor A. Clanton, Amanda M. Lewis, Xinghui Sun, Sylvia Hiller, Phillip Huynh, Jennifer Wilder, John Hagaman, Feng Li, Nobuyo Maeda-Smithies, Ed Harris

Department of Biochemistry: Faculty Publications

We have previously identified a novel atherosclerosis quantitative trait locus (QTL), Arch atherosclerosis 5 (Aath5), on mouse chromosome 10 by three-way QTL analyses between Apoe^−/− mice on a DBA/2J, 129S6 and C57BL/6J background. The DBA/2J haplotype at the Aath5 locus was associated with smaller plaque size. One of the candidate genes underlying Aath5 was Stabilin-2 (Stab2), which encodes a clearance receptor for hyaluronan (HA) predominantly expressed in liver sinusoidal endothelial cells (LSECs). However, the role of Stab2 in atherosclerosis is unknown. A congenic line of Apoe^−/− mice carrying Aath5 covering the Stab2 …

The Ability To Classify Patients Based On Gene-Expression Data Varies By Algorithm And Performance Metric, Stephen Piccolo, Avery Mecham, Nathan P. Golightly, Jérémie L. Johnson, Dustin B. Miller

Faculty Publications

By classifying patients into subgroups, clinicians can provide more effective care than using a uniform approach for all patients. Such subgroups might include patients with a particular disease subtype, patients with a good (or poor) prognosis, or patients most (or least) likely to respond to a particular therapy. Transcriptomic measurements reflect the downstream effects of genomic and epigenomic variations. However, high-throughput technologies generate thousands of measurements per patient, and complex dependencies exist among genes, so it may be infeasible to classify patients using traditional statistical models. Machine-learning classification algorithms can help with this problem. However, hundreds of classification algorithms exist—and …

Effects Of High-Volume Versus High-Load Resistance Training On Skeletal Muscle Growth And Molecular Adaptations, Christopher G. Vann, Casey L. Sexton, Shelby C. Osburn, Morgan A. Smith, Cody T. Haun, Melissa N. Rumbley, Petey W. Mumford, Nathan T. Montgomery, Bradley A. Ruple, James Mckendry, Jonathan Mcleod, Adil Bashir, Ronald J. Beyers, Matthew S. Brook, Kenneth Smith, Philip J. Atherton, Darren T. Beck, James R. Mcdonald, Kaeilin C. Young, Stuart M. Phillips, Michael D. Roberts

Faculty Scholarship

We evaluated the effects of higher-load (HL) versus (lower-load) higher-volume (HV) resistance training on skeletal muscle hypertrophy, strength, and muscle-level molecular adaptations. Trained men (n = 15, age: 23 ± 3 years; training experience: 7 ± 3 years) performed unilateral lower-body training for 6 weeks (3× weekly), where single legs were randomly assigned to HV and HL paradigms. Vastus lateralis (VL) biopsies were obtained prior to study initiation (PRE) as well as 3 days (POST) and 10 days following the last training bout (POSTPR). Body composition and strength tests were performed at each testing session, and biochemical assays were …

Reduction Of Stabilin-2 Contributes To A Protection Against Atherosclerosisstabilin, Yukako Kayashima1*,, Conner A. Clanton, Amanda M. Lewis, Xinghui Sun, Sylvia Hiller, Phillip Huynh, Jennifer Wilder, John Hagaman, Feng Li, Nobuyo Maeda-Smithies, Edward N. Harris

Department of Biochemistry: Faculty Publications

We have previously identified a novel atherosclerosis quantitative trait locus (QTL), Arch atherosclerosis 5 (Aath5), on mouse chromosome 10 by three-way QTL analyses between Apoe^−/− mice on a DBA/2J, 129S6 and C57BL/6J background. The DBA/2J haplotype at the Aath5 locus was associated with smaller plaque size. One of the candidate genes underlying Aath5 was Stabilin-2 (Stab2), which encodes a clearance receptor for hyaluronan (HA) predominantly expressed in liver sinusoidal endothelial cells (LSECs). However, the role of Stab2 in atherosclerosis is unknown. A congenic line of Apoe^−/− mice carrying Aath5 covering the Stab2^DBA allele …

Deepreal: A Deep Learning Powered Multi-Scalemodeling Framework For Predicting Out-Of-Distributionligand-Induced Gpcr Activity, Tian Cai, Kyra Alyssa Abbu, Yang Liu, Lei Xie

Publications and Research

Motivation Drug discovery has witnessed intensive exploration of predictive modeling of drug–target physical interactions over two decades. However, a critical knowledge gap needs to be filled for correlating drug–target interactions with clinical outcomes: predicting genome-wide receptor activities or function selectivity, especially agonist versus antagonist, induced by novel chemicals. Two major obstacles compound the difficulty on this task: known data of receptor activity is far too scarce to train a robust model in light of genome-scale applications, and real-world applications need to deploy a model on data from various shifted distributions.

Results To address these challenges, we have developed an end-to-end …

A Novel Proton-Integrating Radiography System Design Using A Monolithic Scintillator Detector: Experimental Studies, Chinmay D Darne, Daniel G Robertson, Fahed Alsanea, Charles-Antoine Collins-Fekete, Sam Beddar

Faculty, Staff and Student Publications

Research on proton-based imaging systems aims to improve treatment planning, internal anatomy visualization, and patient alignment for proton radiotherapy. The purpose of this study was to demonstrate a new proton radiography system design consisting of a monolithic plastic scintillator volume and two optical cameras for use with scanning proton pencil beams. Unlike the thin scintillating plates currently used for proton radiography, the plastic scintillator volume (20 × 20 × 20 cm

Synergy Of Venetoclax And 8-Chloro-Adenosine In Aml: The Interplay Of Rrna Inhibition And Fatty Acid Metabolism, Dinh Hoa Hoang, Corey Morales, Ivan Rodriguez Rodriguez, Melissa Valerio, Jiamin Guo, Min-Hsuan Chen, Xiwei Wu, David Horne, Varsha Gandhi, Lisa S Chen, Bin Zhang, Vinod Pullarkat, Steven T Rosen, Guido Marcucci, Ralf Buettner, Le Xuan Truong Nguyen

Faculty, Staff and Student Publications

It is known that 8-chloro-adenosine (8-Cl-Ado) is a novel RNA-directed nucleoside analog that targets leukemic stem cells (LSCs). In a phase I clinical trial with 8-Cl-Ado in patients with refractory or relapsed (R/R) AML, we observed encouraging but short-lived clinical responses, likely due to intrinsic mechanisms of LSC resistance. LSC homeostasis depends on amino acid-driven and/or fatty acid oxidation (FAO)-driven oxidative phosphorylation (OXPHOS) for survival. We recently reported that 8-Cl-Ado and the BCL-2-selective inhibitor venetoclax (VEN) synergistically inhibit FAO and OXPHOS in LSCs, thereby suppressing acute myeloid leukemia (AML) growth in vitro and in vivo. Herein, we report that 8-Cl-Ado …

Assessment Of Diversity Of Antimicrobial Resistance Phenotypes And Genotypes Of Mannheimia Haemolytica Isolates From Bovine Nasopharyngeal Swabs, Hannah F. Carter, Robert W. Wills, Matthew A. Scott, Alexis C. Thompson, Randall S. Singer, John Dustin Loy, Brandi B. Karisch, William B. Epperson, Amelia R. Woolums

School of Veterinary and Biomedical Sciences: Faculty Publications

The threat of bovine respiratory disease (BRD) for cattle operations is exacerbated by increasing prevalence of antimicrobial resistance (AMR) in Mannheimia haemolytica, a leading cause of BRD. Characterization of AMR in M. haemolytica by culture and susceptibility testing is complicated by uncertainty regarding the number of colonies that must be selected to accurately characterize AMR phenotypes (antibiograms) and genotypes in a culture. The study objective was to assess phenotypic and genotypic diversity of M. haemolytica isolates on nasopharyngeal swabs (NPS) from 28 cattle at risk for BRD or with BRD. NPS were swabbed onto five consecutive blood agar plates; …

Physiological Responses To Acute Cycling With Blood Flow Restriction, Matthew A. Kilgas, Tejin Yoon, John Mcdaniel, Kevin Phillips, Steve Elmer

Michigan Tech Publications, Part 1

Aerobic exercise with blood flow restriction (BFR) can improve muscular function and aerobic capacity. However, the extent to which cuff pressure influences acute physiological responses to aerobic exercise with BFR is not well documented. We compared blood flow, tissue oxygenation, and neuromuscular responses to acute cycling with and without BFR. Ten participants completed four intermittent cycling (6 × 2 min) conditions: low-load cycling (LL), low-load cycling with BFR at 60% of limb occlusion pressure (BFR60), low-load cycling with BFR at 80% of limb occlusion pressure (BFR80), and high-load cycling (HL). Tissue oxygenation, cardiorespiratory, metabolic, and perceptual responses were assessed during …

Iowa Wine Industry And Its Educational Needs, Aude A. Watrelot

Journal of Extension

The grape and wine industry in Iowa is based on non-Vitis vinifera grapes, which are not well-known and come with their own set of challenges. An industry survey revealed that wine business and marketing are the main concerns for the Iowa grape and wine industry members. The educational resources already provided by Extension personnel were highly appreciated and events should be offered in several locations around Iowa. The main topics of roundtables and discussions should be about vineyard management and winemaking practices, specifically practices to avoid microbial spoilage, to reduce wine acidity and to select appropriate yeasts for fermentation.

Thinking Outside The Box Of Evaluation Platforms: Adapting Qualtrics For Virtual 4-H Judging Competitions, Maria Calvert, Ty Peckman

Journal of Extension

Competitive events represent a major component of 4-H programming. These events are typically held in person; however, unforeseen circumstances, like the COVID-19 pandemic, have required Extension professionals to adapt these programs to virtual settings. This paper outlines some unique features of Qualtrics, a web-based tool commonly used to build surveys, that led Missouri 4-H to use the platform to administer agriculture and natural resource science contests.

Water Conservation: Extension Agents’ Perceptions Of Issue Importance, Professional Abilities, And Landowner Needs, Audrey Mccrary, Leslie M. Burger, Laura Downey, Beth H. Baker

Journal of Extension

The Extension Service is one of many agencies charged with increasing awareness and knowledge of research-based agricultural conservation practices. A regional survey of Extension agents with agriculture and natural resources responsibilities was conducted to assess the need for in-service training on 11 water resource conservation topics. The highest priority training needs were for topics related to complex interactions and drivers of agricultural water pollution. This article highlights the implications of these results and offers broader perspective on bringing the Borich model of needs assessment into the agricultural and natural resources realm of subject matter expertise.

A Revision Of The Genus Mesobuthus Vachon, 1950, With A Description Of 14 New Species (Scorpiones: Buthidae), František Kovařík, Victor Fet, Benjamin Gantenbein, Matthew R. Graham, Ersen A. Yağmur, František Šťáhlavský, Nikita M. Poverennyi, Nizami E. Novruzov

Euscorpius

The Asian genus Mesobuthus Vachon, 1950, s. str. (Buthidae) (also known as an informal ‘M. eupeus complex’), which includes the most common scorpion species found from Turkey to China, is revised based on new collections, mainly from Central Asia, Turkey, and Iran, using both morphological and mitochondrial DNA data. Available type specimens of all species were studied; neotypes and lectotypes were designated when necessary. The type species Mesobuthus eupeus (C. L. Koch, 1839), s. str., is restricted to the Caucasus Mts. Currently, the genus includes 29 valid species. Fifteen previously described taxa are recognized as species: Mesobuthus afghanus (Pocock, …

Examining Farm Financial Management: How Do Small Us Farms Meet Their Agricultural Expenses?, Omobolaji Omobitan, Aditya R. Khanal

Agricultural and Environmental Sciences Faculty Research

Small farms in the US have significant challenges in financial management. This study examines how small farmers undertake farm financial management to meet their agricultural and farm-related spending and expenses. Using primary survey data from Tennessee, the study investigates the factors influencing the extent of use of five financing sources to meet the spending and expenses: cash/fund directly generated from the sale of agricultural products, farmer’s past savings, farm household’s off-farm income, income/incentives from government payments, and external loans. Using negative binomial regression estimation of generalized linear models, findings suggest that the decision on the use of financing sources is …

Employment With The Northwest Territories Aquatics Program: A Significant Life Event?, Sebastien Denize, Audrey R. Giles

International Journal of Aquatic Research and Education

For over 60 years, the Northwest Territories Aquatics Program (NWTAP) has employed university students from southern Canada with little to no exposure to northern Canada to run seasonal swimming pools and waterfronts in isolated northern communities. In this article, we examine the impact that working for this program had on nine former employees’ lives and whether or not working for the NWTAP could be categorized as a significant life experience. We used thematic analysis of the data from retrospective semi-structured interviews and open-ended questionnaires and identified four themes: (1) employment by the NWTAP enhanced the employees’ self-confidence, independence, and self-efficacy; …

Content, Construct, And Criterion Validity, Reliability, And Objectivity For Aquatic Readiness Assessment For Brazilian Children, Nadia C. Valentini, Keila R G Pereira, Glauber C. Nobre

International Journal of Aquatic Research and Education

The Aquatic Readiness Assessment (ARA) is an assessment instrument for measuring children's aquatic readiness. The objective of the study was to translate the English version into Portuguese and to investigate the content, construct, and criterion validity as well as the reliability and rater objectivity of the ARA for Brazilian children. Twenty-three professionals and 464 children, newborn to 13 years-old participated in the study. We found strong content (94% to 100% of judges’ agreement) and criterion validity, internal consistency (α from .96 to .97), and inter-rater objectivity (ICC from .81 to .98), and test-retest reliability (ICC from .94 to .98). Appropriate …

Variants Within Genes Edil3 And Adgrb3 Are Associated With Divergent Fecal Egg Counts In Katahdin Sheep At Weaning, Gabrielle M. Becker, Joan M. Burke, Ronald M. Lewis, James E. Miller, James L.M. Morgan, Benjamin D. Rosen, Curtis P. Van Tassell, David R. Notter, Brenda M. Murdoch

Department of Animal Science: Faculty Publications

Gastrointestinal nematodes (GIN) pose a severe threat to sheep production worldwide. Anthelmintic drug resistance coupled with growing concern regarding potential environmental effects of drug use have demonstrated the necessity of implementing other methods of GIN control. The aim of this study was to test for genetic variants associated with resistance or susceptibility to GIN in Katahdin sheep to improve the current understanding of the genetic mechanisms responsible for host response to GIN. Linear regression and casecontrol genome-wide association studies were conducted with high-density genotype data and cube-root transformed weaning fecal egg counts (tFEC) of 583 Katahdin sheep. The casecontrol GWAS …

Ability Of Lifeguards To Detect Submerged Manikins In Public Swimming Pool Environments, Élie Vignac, Pascal Lebihain, Brice Guignard, Natacha Heutte, Loic Le Minor, Bastien Soulé

International Journal of Aquatic Research and Education

To prevent drownings in public swimming pools (PSP), French legislation requires constant surveillance by state-certified lifeguards. While previous research showed that surveillance was not always effective, this article focuses on efficiency of surveillance when it is effective. We evaluated the ability of 4 volunteer professional lifeguards to detect a submerged manikin under controlled conditions. One hundred and eight (108) tests were carried out in 2 PSP. Four variables were controlled (i.e., distance, depth, surveillance station, attendance). Our results showed that rapid drowning detection was not exclusively linked to the individual detection capabilities of a lifeguard, but rather it emerged from …

Towards Economic Growth And Development: The Role Of Development Finance Institutions In Fostering Foreign Direct Investment In Sub-Saharan Africa, Carmen Cagiza

Doctoral Projects

This dissertation contributes to the debate on economic development in developing countries through the analyses of four related strands of literature – the FDI and growth literature, the literature on finance and development, the DFI and growth literature, aid, institutions and economic development. The present research seeks to contribute to the debate on the effect of DFIs on FDI and consequently on economic development in developing economies to bridge the existing funding gap and thus ensure these economies achieve the global shared vision of sustainable development of the United Nations 2030 agenda. We acknowledge that development is triggered by economic …

Plants Used By Nyamwezi Traditional Health Practitioners To Remedy Sexually Transmitted Infections In Sikonge, Tanzania, David S. Kacholi, Halima Amir

Journal of Humanities and Social Sciences

This study aims to document medicinal plants used by Nyamwezi traditional health practitioners (NTHPs) in managing sexually transmitted infections (STIs) in Sikonge District. The data on medicinal plants were collected using a semi-structured questionnaire. A total of 23 NTHPs were interviewed. The findings revealed that 28 medicinal plants, belonging to 16 families and 25 genera, are used for the management of STIs in Sikonge. Most species belonged to family Fabaceae (17.9%), Apocynaceae, Combretaceae and Euphorbiaceae (10.7% each), and Olacaceae and Rutaceae (7.1% each). Out of the recorded plants, 45.7% are used to manage gonorrhoea, 25.7% syphilis, 17.1% vaginal candidiasis, 8.6% …

Application Of Pheromone Is Potential Ecological Approach In Management Of Tuta Absoluta In Tomatoes: The Case Of Ruaha River Basin In Iringa, Tanzania, Theonest Kombe, Nicodemus D. Matojo, Fikira Kimbokota

Journal of Humanities and Social Sciences

Tomato leaf miner, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae), is currently considered to be one of the most destructive insect pests of tomatoes worldwide. The present study was conducted in three study sites along Ruaha River Basin, Iringa, Tanzania, to assess community awareness about T. absoluta infestation and its management. The study also evaluated the susceptibility of three tomato varieties against T. absoluta in a greenhouse setting, as well as the effect of colours of traps and doses of pheromone in attracting T. absoluta in a field environment. The field survey revealed that 97% of the respondents were aware of the …

Nonhistone Lysine Methylation As A Protein Degradation Signal, Nicholas A. Lehning, Brad E. Morrison

Biology Faculty Publications and Presentations

Protein degradation is a fundamental feature of cellular life, and malfunction of this process is implicated in human disease. Ubiquitin tagging is the best characterized mechanism of targeting a protein for degradation; however, there are a growing number of distinct mechanisms which have also been identified that carry out this essential function. For example, covalent tagging of proteins with sequestosome-1 targets them for selective autophagy. Degradation signals are not exclusively polypeptides such as ubiquitin, NEDD8, and sequestosome-1. Phosphorylation, acetylation, and methylation are small covalent additions that can also direct protein degradation. The diversity of substrate sequences and overlap with other …

Developing Activities On Uncharismatic Animals Found At Omaha's Henry Doorly Zoo And Aquarium, Justin Hultquist

Honors Program: Senior Projects (Public)

The Education Department at Omaha’s Henry Doorly Zoo and Aquarium has a number of educational activities based on charismatic species found there. I wished to develop activities the department may use for less charismatic or misunderstood species at the zoo. I began by surveying the exhibits at the zoo and taking note of what species or groups didn’t receive as much visitor engagement, or what comments were made on species that were incorrect. I then began to brainstorm and narrow down potential species that I could develop an educational activity around. As I began to develop and create activities, a …

Role Of Bmi1 In Acute Lung Injury, María Helena Hernández-Cuervo

USF Tampa Graduate Theses and Dissertations

Acute Lung Injury (ALI) is a set of signs and symptoms that lead to acute hypoxemic respiratory failure characterized by bilateral pulmonary infiltrates not attributed to cardiogenic origin. It is caused by a massive innate immune response, with the migration of white blood cells (neutrophils and macrophages principally) and a cytokine storm, followed by alterations in mitochondrial function, increase in reactive oxygen species production, and oxidative stress that in turn induces more mitochondrial damage. Several studies have shown that mitochondrial alterations are key events in the mechanism of ALI and reducing mitochondrial dysfunction could be a possible target in the …

Cell-Free Dna Methylation Signatures In Cancer Detection And Classification, Jinyong Huang

USF Tampa Graduate Theses and Dissertations

Early detection of cancer is believed as one of the best solutions to improve the therapeutic outcomes and overall survival of cancer patients. Analysis of circulating nucleic acids in bodily fluids, referred to as “liquid biopsies”, is rapidly gaining prominence for this purpose. Cell-free DNA (cfDNA) methylation has emerged as a promising biomarker for early cancer detection, tumor type classification, and treatment response monitoring. Currently, most cfDNA methylation profiling technologies are based on bisulfite conversion, while enrichment-based methods such as cfMeDIP-seq are beginning to show potential. To expand the use of enrichment-based methods in cfDNA methylation profiling, here, we report …

Determining Pairwise Interactions To Predict Species Interactions In A Complex Community Of Gut Bacteria., Makenzie Maroney

Honors Program: Senior Projects (Public)

The human gastrointestinal (GI) tract houses a diverse and expansive microbial community known as the microbiota. The relationship between the host and the microbiota is considered to be mutualistic, as trillions of bacteria that reside in the hospitable GI tract can assist in essential host functions, including contributing to metabolism and immunity against disease. An important subpopulation of the GI microbiota is the mucin-associated biofilm that is comprised of a dense layer of bacteria that forms on the GI mucin layer. The mucin layer provides an alternative niche to the GI lumen; the glycans of the mucin and the subsequent …

Genetic Variation Of The Mitochondrial Dna Control Region Across Plains Bison Herds In Usa And Canada, Gaimi Davies, Blake Mccann, Lee Jones, Stefano Liccioli, Maria Cecilia Penedo, Igor V. Ovtchinnikov

Biology Faculty Publications

Once numbering in the tens of millions, bison (Bison bison) have faced a broad range of challenges over the past century, including genetic impacts from the population bottleneck in the 1800s, and significant loss and fragmentation of habitat resulting in little opportunity for population growth on remaining small, geographically isolated reserves. To identify best practices for bison stewardship against this backdrop, managers must understand the genetic composition of existing conservation herds. This study characterized 14 plains bison (Bison bison bison) herds managed by the U.S. Department of Interior and Parks Canada Agency based on complete mtDNA …