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Articles 53791 - 53820 of 255701

Full-Text Articles in Life Sciences

Embryological Development And Mortality Of Danio Rerio In Long Term Exposure To 17Α-Ethynylestradiol, Seong A. Jeon Mar 2022

Embryological Development And Mortality Of Danio Rerio In Long Term Exposure To 17Α-Ethynylestradiol, Seong A. Jeon

University Honors Theses

Synthetic estrogens as well as other chemicals have become an increasing issue in the impact they have on marine species particularly those sensitive to the environment. A broadly used synthetic estrogen, 17α-ethynylestradiol, has become more widely known to become a growing problem to the ecology of marine wildlife due to increased usage and lack of removal in municipal sewage treatment facilities. This study observes the impact of low level EE2 in embryos following generational exposure. Zebrafish embryos, following three generations of exposure, were observed in rate of mortality, lethality, and hatch success. Concentrations as low as 1ng/L of EE2 has …

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A Farm-To-Fork Quantitative Microbial Exposure Assessment Of Β-Lactam-Resistant Escherichia Coli Among U.S. Beef Consumers, Yangjunna Zhang, John W. Schmidt, Terrance M. Arthur, Tommy L. Wheeler, Qi Zhang, Bing Wang Mar 2022

A Farm-To-Fork Quantitative Microbial Exposure Assessment Of Β-Lactam-Resistant Escherichia Coli Among U.S. Beef Consumers, Yangjunna Zhang, John W. Schmidt, Terrance M. Arthur, Tommy L. Wheeler, Qi Zhang, Bing Wang

Department of Food Science and Technology: Faculty Publications

Integrated quantitative descriptions of the transmission of β-lactam-resistant Escherichia coli (BR-EC) from commercial beef products to consumers are not available. Here, a quantitative microbial exposure assessment model was established to simulate the fate of BR-EC in a farm-to-fork continuum and provide an estimate of BR-EC exposure among beef consumers in the U.S. The model compared the per-serving exposures from the consumption of intact beef cuts, non-intact beef cuts, and ground beef. Additionally, scenario analysis was performed to evaluate the relative contribution of antibiotic use during beef cattle production to the level of human exposure to BR-EC. The model predicted mean …

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A Non-Iteration Solution For Solving The Backward-In-Time Two-Dimensional Burgers’ Equation With A Large Reynolds Number, Yen-Shen Chang, Yung-Wei Chen, Chein-Shan Liu, Jiang-Ren Chang Mar 2022

A Non-Iteration Solution For Solving The Backward-In-Time Two-Dimensional Burgers’ Equation With A Large Reynolds Number, Yen-Shen Chang, Yung-Wei Chen, Chein-Shan Liu, Jiang-Ren Chang

Journal of Marine Science and Technology–Taiwan

This article proposes a noniteration solution based on the Lie-group shooting method (LGSM) to solve the backward-in-time two-dimensional Burgers’ equation with a large Reynolds number. The backward problem is famous for seriously ill-posed cases because the solution is generally unstable and highly dependent on the input data. Small perturbations in the input data, such as random errors inherent to the measurements in the analysis, can cause large oscillations in the solution. To handle a large Reynolds number under long time spans, it is very difficult to integrate towards the time direction. To avoid time integration and numerical iteration, the noniteration …

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Polyketides And Terpenes In Marine Microalgae: Ecological Roles And Mechanisms Of Biosynthesis, Yunxuan Xie, Mohammad Elsheikh Mar 2022

Polyketides And Terpenes In Marine Microalgae: Ecological Roles And Mechanisms Of Biosynthesis, Yunxuan Xie, Mohammad Elsheikh

Journal of Marine Science and Technology–Taiwan

Marine microalgae comprise a large number of polyketides and terpenes identified to date with highly diverse molecular scaffolds and the biological activities (Kalaitzis et al., 2010). These compounds play significant roles in marine food web and nutraceutical supplementation in marine animals. Compounds of this class are often synthesized by gene clusters with dedicated domains responsible for carbon chain extension and modification. The unique molecular structure of these compounds encourages people to investigate their biosynthetic mechanisms in-depth. In this review, we summarize relevant articles to unravel the shared mechanisms of these compounds to further understand the variety of domain organizations and …

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Unbaited Light-Emitting Diode Traps Performance For Catching Orange Mud Crabs, Muhamad Naimullah, Kuo-Wei Lan, Mhd Ikhwanuddin, Adnan Amin-Safwan, Wei-Yu Lee Mar 2022

Unbaited Light-Emitting Diode Traps Performance For Catching Orange Mud Crabs, Muhamad Naimullah, Kuo-Wei Lan, Mhd Ikhwanuddin, Adnan Amin-Safwan, Wei-Yu Lee

Journal of Marine Science and Technology–Taiwan

Mud crabs (Scylla spp.) are a vital fishery resource and targeted for a valuable source of income for coastal communities throughout the Indo-Pacific region. Baited traps are a considerable expense in crustacean fishing. Hence, the present study was performed to investigate the catchability of Scylla olivacea in response to light-emitting diodes (LEDs) in captivity and field conditions. We used a new experimental setup in the captivity condition that revealed most S. olivacea were attracted to green, blue, and white LED lights and no attractive effect by red LED lights similar to the controls. Field studies have shown that the catch …

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Catch And Effort Standardization For Taiwanese Swordtip Squid Uroteuthis Edulis Fisheries In The Southern East China Sea, Ke-Yang Chang, Yu-Jie Hsu, Tzu-Yun Ching, Cheng-Hsin Liao, Chih-Shin Chen Mar 2022

Catch And Effort Standardization For Taiwanese Swordtip Squid Uroteuthis Edulis Fisheries In The Southern East China Sea, Ke-Yang Chang, Yu-Jie Hsu, Tzu-Yun Ching, Cheng-Hsin Liao, Chih-Shin Chen

Journal of Marine Science and Technology–Taiwan

The swordtip squid Uroteuthis edulis is a neritic squid species that is widely distributed within the coastal waters of subtropical and tropical Indo-western Pacific regions. This squid is a commercially important species throughout its distribution range and plays a critical role in marine ecosystems. Stock assessments of the squid in the East China Sea (ECS) are scarce, although it is crucial information for the sustainability of the squid and relevant fisheries. The squid was harvested by various fisheries in the southern ECS, which could restrict methods for catch-effort standardization. In this study, catch-effort data of Taiwanese swordtip squid fisheries in …

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Uniform Torsion Analysis Of Composite Shafts Using Point Collocation Method Based On Pascal Polynomials, Chih-Wen Chang, Jyh-Hong Wu, Ying-Ru Lin, Yu-Feng Lin, Nan-Nong Huang Mar 2022

Uniform Torsion Analysis Of Composite Shafts Using Point Collocation Method Based On Pascal Polynomials, Chih-Wen Chang, Jyh-Hong Wu, Ying-Ru Lin, Yu-Feng Lin, Nan-Nong Huang

Journal of Marine Science and Technology–Taiwan

Torsion bars are important elements in the marine structure applications. The usage of the composite torsion bars has increased recently because it takes advantage of the different material property of each constituent. The current research focuses mainly on the uniform warping analysis of composite torsion shafts by using the point collocation method (PCM). The PCM is based on Pascal's triangle polynomial expansion and coupled with subdomain technique that is indispensable for the analysis of composite bars. In addition to satisfying the field equation (Laplace equation for warping function) and traction-free boundary condition for each component, the continuity conditions in both …

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Local Reinforcement Design And Safety Factor Evaluation Of Elliptical Hatch Of Pressure Hull, Guang-Min Luo, Jun-Wei Chen Mar 2022

Local Reinforcement Design And Safety Factor Evaluation Of Elliptical Hatch Of Pressure Hull, Guang-Min Luo, Jun-Wei Chen

Journal of Marine Science and Technology–Taiwan

A pressure hull is the primary pressure-resistant structure of a submarine. To safeguard the compressive strength of such a hull, local structural reinforcement is required for various hatches on the hull. Hatches are commonly reinforced through increasing the thickness of the hull regions around them; alternatively, hatch nozzle walls are connected with stiffened frames to maintain the continuity of reinforced structures. This study focused on a pressure hull with an elliptical hatch. A PD-5500 cylindrical pressure hull was used to design a cylindrical pressure hull structure, and a model of hatch nozzle reinforcement was designed using the Boiler and Pressure …

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Effects Of Dietary Lipid, L-Carnitine And L-Lysine Supplementation On The Growth Performance And Body Composition Of Epinephelus Lanceolatus Larvae, Jen-Hong Chu, Yu-Cong Hong, Angela Chien, Shyn-Shin Sheen Mar 2022

Effects Of Dietary Lipid, L-Carnitine And L-Lysine Supplementation On The Growth Performance And Body Composition Of Epinephelus Lanceolatus Larvae, Jen-Hong Chu, Yu-Cong Hong, Angela Chien, Shyn-Shin Sheen

Journal of Marine Science and Technology–Taiwan

The effects of different combinations of dietary lipid, L-carnitine and L-lysine on the growth performance and fatty acid profiles of larval grouper Epinephalus lanceolatus were evaluated. Eight treatment diets were formulated based on a 2x2x2 factorial design, namely 5 and 14% dietary oil mixture (3:1 fish oil/soybean oil), 0 and 0.5% L-carnitine and 0 and 2.83% L-lysine. Each diet was randomly assigned to triplicate groups of ten 0.08g E. lanceolatus larvae for 42 days. The grouper larvae fed with diets containing 14% lipid had significantly higher weight gain percentages than those fed with diets containing 5% lipid. The weight gain …

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Centralized Project-Specific Metadata Platforms: Toolkit Provides New Perspectives On Open Data Management Within Multi-Institution And Multidisciplinary Research Projects, Andrew Wright Child, Jennifer Hinds, Lucas Sheneman, Sven Buerki Mar 2022

Centralized Project-Specific Metadata Platforms: Toolkit Provides New Perspectives On Open Data Management Within Multi-Institution And Multidisciplinary Research Projects, Andrew Wright Child, Jennifer Hinds, Lucas Sheneman, Sven Buerki

Biology Faculty Publications and Presentations

Open science and open data within scholarly research programs are growing both in popularity and by requirement from grant funding agencies and journal publishers. A central component of open data management, especially on collaborative, multidisciplinary, and multi-institutional science projects, is documentation of complete and accurate metadata, workflow, and source code in addition to access to raw data and data products to uphold FAIR (Findable, Accessible, Interoperable, Reusable) principles. Although best practice in data/metadata management is to use established internationally accepted metadata schemata, many of these standards are discipline-specific making it difficult to catalog multidisciplinary data and data products in a …

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Antimicrobial Activity Of Zinc Oxide Nanoparticles Compared To Silver Nanoparticles Administered Towards Methicillin-Resistant Staphylococcus Aureus Biofilms, Kevin Núño Mar 2022

Antimicrobial Activity Of Zinc Oxide Nanoparticles Compared To Silver Nanoparticles Administered Towards Methicillin-Resistant Staphylococcus Aureus Biofilms, Kevin Núño

USF Tampa Graduate Theses and Dissertations

Zinc oxide nanoparticles (ZnO NPs), silver nanoparticles (AgNPs), and chitosan nanoparticles have strong antibacterial activity as a broad-spectrum antibiotic against multi-drug resistant (MDR) bacteria. MDR bacteria have a mechanism that increases antibiotic resistance through biofilm formations. MDR methicillin-resistant Staphylococcus aureus BAA-1720 was chosen for an in vitro assessment to calculate the biofilm reduction rate and the median effective dose (ED50) by performing biofilm quantification. Dynamic light scattering was chosen to determine the physiochemical characteristics of ZnO NPs, silver nanoparticles (AgNPs), and chitosan NPs. Transmission electron microscopy was conducted to verify each treatment’s physiochemical characteristics. Antimicrobial susceptibility testing of broad-spectrum and …

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Evaluating And Improving A Novel Toolkit For Implementation And Optimization Of Lynch Syndrome Universal Tumor Screening, Tara M. Wolfinger Mar 2022

Evaluating And Improving A Novel Toolkit For Implementation And Optimization Of Lynch Syndrome Universal Tumor Screening, Tara M. Wolfinger

USF Tampa Graduate Theses and Dissertations

Individuals with Lynch syndrome (LS) have a hereditary predisposition to colorectal cancer (CRC), endometrial cancer (EC), and several additional cancer types. Universal tumor screening (UTS) is a systematic approach that increases identification of LS so affected individuals can benefit from targeted therapies and risk-reduction strategies to decrease cancer- related morbidity and mortality. Despite the known benefits of UTS, the practice is widely underutilized, likely due to the complexity of implementation and optimization. We developed a novel evidence-based toolkit to help institutions implement and optimize UTS programs. As part of an iterative evaluation process, we obtained feedback on the toolkit through …

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Western Prairie Fringed Orchid Management, Ecology, And Decline At Mormon Island, Andrew J. Caven Mar 2022

Western Prairie Fringed Orchid Management, Ecology, And Decline At Mormon Island, Andrew J. Caven

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

The western prairie fringed orchid (Platanthera praeclara Sheviak & M. L. Bowles; WPFO) was first detected in a vegetative state on Mormon Island in 1978 and identification was confirmed following a mass flowering event in 1982. From a high count of ~60 plants the WPFO slowly declined and has not been observed since 2000 despite flowering season surveys conducted in 15 of the last 20 years. We explore the natural history of the WPFO in the contexts of Mormon Island to establish potential causes for its apparent disappearance and evaluate the possibility it persists in some capacity. Our investigation …

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Editorial: Genomics-Enabled Triticeae Improvement, Xue-Feng Ma, Xianchun Xia, Shuyu Liu, P. Stephen Baenziger, Hakan Özkan Mar 2022

Editorial: Genomics-Enabled Triticeae Improvement, Xue-Feng Ma, Xianchun Xia, Shuyu Liu, P. Stephen Baenziger, Hakan Özkan

Department of Agronomy and Horticulture: Faculty Publications

No abstract provided.

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Fusionai, A Dna-Sequence-Based Deep Learning Protocol Reduces The False Positives Of Human Fusion Gene Prediction, Pora Kim, Hua Tan, Jiajia Liu, Himansu Kumar, Xiaobo Zhou Mar 2022

Fusionai, A Dna-Sequence-Based Deep Learning Protocol Reduces The False Positives Of Human Fusion Gene Prediction, Pora Kim, Hua Tan, Jiajia Liu, Himansu Kumar, Xiaobo Zhou

Faculty, Staff and Student Publications

Even though there were many tool developments of fusion gene prediction from NGS data, too many false positives are still an issue. Wise use of the genomic features around the fusion gene breakpoints will be helpful to identify reliable fusion genes efficiently. For this aim, we developed FusionAI, a deep learning pipeline predicting human fusion gene breakpoints from DNA sequence. FusionAI is freely available via https://compbio.uth.edu/FusionGDB2/FusionAI. For complete details on the use and execution of this protocol, please refer to Kim et al. (2021b).

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Paradoxical Effects Of Osteoprotegerin On Vascular Function: Inhibiting Inflammation While Promoting Oxidative Stress?, Nhat-Tu Le, Elizabeth A Olmsted-Davis, Jun-Ichi Abe Mar 2022

Paradoxical Effects Of Osteoprotegerin On Vascular Function: Inhibiting Inflammation While Promoting Oxidative Stress?, Nhat-Tu Le, Elizabeth A Olmsted-Davis, Jun-Ichi Abe

Faculty, Staff and Student Publications

Osteoprotegerin (OPG), also known as osteoclastogenesis inhibitory factor or tumor necrosis factor receptor superfamily member 11B, is well known as a modulator of bone remodeling. The contribution of OPG to cardiovascular disease (CVD) has been suggested, but its molecular mechanism is complex and remains unclear. In the present study, Alves-Lopes et al. (Clin. Sci. (Lond.) (2021) 135(20): https://doi.org/10.1042/CS20210643) reported the critical role of syndecan-1 (SDC-1, also known as CD138), a surface protein part of the endothelial glycocalyx, in OPG-induced vascular dysfunction. The authors found that in endothelial cells (ECs), through SDC-1, OPG increased eNOS Thr495 phosphorylation, thereby inhibiting eNOS activity. …

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Analysis Of Sir Epidemic Models With Sociological Phenomenon, Robert F. Allen, Katherine C. Heller, Matthew A. Pons Mar 2022

Analysis Of Sir Epidemic Models With Sociological Phenomenon, Robert F. Allen, Katherine C. Heller, Matthew A. Pons

Spora: A Journal of Biomathematics

We propose two SIR models which incorporate sociological behavior of groups of individuals. It is these differences in behaviors which impose different infection rates on the individual susceptible populations, rather than biological differences. We compute the basic reproduction number for each model, as well as analyze the sensitivity of R0 to changes in sociological parameter values.

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The Large And Small Of It: The Microbiome And Metagenomics, Austin Hopkins, Elaina Gollmar, Jessica Fernandez, Shawn Wolf, Austin Hilverding, Andrew M. Roecker Mar 2022

The Large And Small Of It: The Microbiome And Metagenomics, Austin Hopkins, Elaina Gollmar, Jessica Fernandez, Shawn Wolf, Austin Hilverding, Andrew M. Roecker

Pharmacy and Wellness Review

Metagenomics, the analysis of the microbial genome, permits scientists to understand the influences of external sources including diet, metabolism and antibiotics on the human microbiome. Research has revealed the possibility of a core symbiosis between humans and bacteria. The main role of the human microbiome is to aid in digestion, but identified ancillary roles include immunologic homeostasis and infection prevention. Quantifying the composition and variability of the microbiome will help lead to future treatments or preventive strategies against unhealthy change. A variety of methods may be used to define the microbiome, and 16S amplicon sequencing is primarily utilized today. Probiotics …

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Tackling Protein-Calorie Malnutrition During World Crises, Zahra Ali Padhani, Jai K. Das, Saeed Akhtar, Tariq Ismail, Zulfiqar Ahmed Bhutta Mar 2022

Tackling Protein-Calorie Malnutrition During World Crises, Zahra Ali Padhani, Jai K. Das, Saeed Akhtar, Tariq Ismail, Zulfiqar Ahmed Bhutta

Woman and Child Health

Undernutrition is still highly prevalent in developing countries and leads to a multitude of problems as it weakens the immune system, which leads to increased risk of infections and diet-related diseases. COVID-19 has worsened the existing situation and has resulted in unprecedented health, social, and economic disruptions across the world. Before COVID-19, about 54% children under 5 years were moderately or seriously malnourished, and after the COVID-19 pandemic, early estimates suggest that an additional 2.6 million children were stunted; 9.3 million were wasted, with an addition of 2.1 million maternal anemia cases; 168,000 child deaths; and USD 29.7 billion in …

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Behavioral And Physiological Effects Of Heavy Metals On Fish: A Review And Preliminary Results, Diana V. Morales Mar 2022

Behavioral And Physiological Effects Of Heavy Metals On Fish: A Review And Preliminary Results, Diana V. Morales

Theses and Dissertations

Pollution, specifically heavy metal pollution, in various bodies of water has been a significant issue for decades. Research has been conducted on heavy metals and their effects on the environment, larger fish species, and humans since the 1970s (Skidmore, 1964; Adeniyi et al., 2007; Adeosun et al., 2015; Bawuro et al., 2018). It is known that zinc is a neurotoxin in some species and causes harm to the environment. It is also known that it causes harm to people if it is ingested or if they are exposed to it for long periods of time (Mahurpawar, 2015; Mizuno and Kawahara, …

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Using Fine-Scale Aquatic Habitat Data To Construct Dreissenid Sdms In The Laurentian Great Lakes, Grace C. Henderson Mar 2022

Using Fine-Scale Aquatic Habitat Data To Construct Dreissenid Sdms In The Laurentian Great Lakes, Grace C. Henderson

USF Tampa Graduate Theses and Dissertations

The invasion of the Laurentian Great Lakes by aquatic invasive species (AIS) has been the subject of investigation for decades, due to their dramatic alterations to the ecosystem and high economic costs. Two AIS with the largest impacts are dreissenid zebra and quagga mussels, and though these species have been studied extensively, questions remain about what factors control their distributions, and whether lake warming will alter these distributions. Species distribution models (SDMs) offer a powerful tool to examine the relationship between species presences and environmental variables, which are typically bioclimactic data. The creation of the Aquatic Habitat (AqHab) dataset containing …

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Inaccuracies In Patient Self-Report Of Genetic Testing Results For Hereditary Cancer Risks Could Impact Risk-Management Practices, Brittany Faye Sears Mar 2022

Inaccuracies In Patient Self-Report Of Genetic Testing Results For Hereditary Cancer Risks Could Impact Risk-Management Practices, Brittany Faye Sears

USF Tampa Graduate Theses and Dissertations

Pathogenic variants (PV) or likely pathogenic variants (LPV) in a cancer risk gene increase lifetime risks of developing cancer. National guidelines provide evidence-based recommendations on cancer risk management (CRM) strategies tailored to the cancer risks associated with PV/LPV in different genes. Emotional responses after learning of a PV/LPV have been studied as predictors of patient adherence to CRM, but less attention has been given to whether patients remember their actual genetic test results and the impact this may have on subsequent adherence to CRM. We surveyed a group of 114 participants registered with the Inherited Cancer Registry (ICARE), all of …

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The Collateral Effects Of Covid-19 Pandemic On The Status Of Carbapenemase-Producing Pathogens, Carole Ayoub Moubareck, Dalal Hammoudi Halat Mar 2022

The Collateral Effects Of Covid-19 Pandemic On The Status Of Carbapenemase-Producing Pathogens, Carole Ayoub Moubareck, Dalal Hammoudi Halat

All Works

The serious challenge of antimicrobial resistance continues to threaten public health and lingers in the era of the coronavirus disease 2019 (COVID-19), declared pandemic by the World Health Organization. While the pandemic has triggered the importance of infection control practices and preventive measures such as physical distancing, hand hygiene, travel reduction and quarantine, the ongoing alarm of antimicrobial resistance seems to accompany the pandemic too. Antimicrobial resistance has been fostered during COVID-19, possibly due to high rate of empirical antibiotic utilization in COVID-19 patients, increased use of biocides, and the disruption of proper healthcare for other conditions. Specifically, carbapenemase-producing Gram-negative …

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Prmt5 Interacting Partners And Substrates In Oligodendrocyte Lineage Cells, David K. Dansu, Jialiang Liang, Ipek Selcen, Haiyan Zheng, Dirk F. Moore, Patricia Casaccia Mar 2022

Prmt5 Interacting Partners And Substrates In Oligodendrocyte Lineage Cells, David K. Dansu, Jialiang Liang, Ipek Selcen, Haiyan Zheng, Dirk F. Moore, Patricia Casaccia

Advanced Science Research Center

The protein arginine methyl transferase PRMT5 is an enzyme expressed in oligodendrocyte lineage cells and responsible for the symmetric methylation of arginine residues on histone tails. Previous work from our laboratory identified PRMT5 as critical for myelination, due to its transcriptional regulation of genes involved in survival and early stages of differentiation. However, besides its nuclear localization, PRMT5 is found at high levels in the cytoplasm of several cell types, including oligodendrocyte progenitor cells (OPCs) and yet, its interacting partners in this lineage, remain elusive. By using mass spectrometry on protein eluates from extracts generated from primary oligodendrocyte lineage cells …

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Assessing Reported Use Of Clinical Genetic Counseling Skills, Nicole M. Wright Mar 2022

Assessing Reported Use Of Clinical Genetic Counseling Skills, Nicole M. Wright

USF Tampa Graduate Theses and Dissertations

Genetic Counseling (GC) is striving to become a more evidence-based field, but the absence of a clearly defined taxonomy of clinical skills is impeding efforts to systematically study and compare GC processes. Several frameworks such as the Reciprocal Engagement Model (REM) and FOCUS-GC have outlined strategies and behaviors used to meet patient needs, but neither include comprehensive descriptions of existing skills or delineate which are actually being used most commonly in clinical practice. The purpose of this study was to compile a large list of demonstrable genetic counseling skills and determine how often clinical genetic counselors (GCs) report using them …

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Acute And Chronic Bone Marker And Endocrine Responses To Resistance Exercise With And Without Blood Flow Restriction In Young Men, Debra A. Bemben, Vanessa D. Sherk, Samuel R. Buchanan, Sojung Kim, Kyle Sherk, Michael G. Bemben Mar 2022

Acute And Chronic Bone Marker And Endocrine Responses To Resistance Exercise With And Without Blood Flow Restriction In Young Men, Debra A. Bemben, Vanessa D. Sherk, Samuel R. Buchanan, Sojung Kim, Kyle Sherk, Michael G. Bemben

Health & Human Performance Faculty Publications

In this study, we compared acute and chronic bone marker and hormone responses to 6 weeks of low intensity (20% 1RM) blood flow restriction (BFR20) resistance training to high intensity (70% 1RM) traditional resistance training (TR70) and moderate intensity (45% 1RM) traditional resistance training (TR45) in young men (18–35 years). Participants were randomized to one of the training groups or to a control group (CON). The following training programs were performed 3 days per week for 6 weeks for knee extension and knee flexion exercises: BFR20, 20%1RM, 4 sets (30, 15, 15, 15 reps) wearing blood flow restriction cuffs around …

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Proteomic Profiling Based Classification Of Cll Provides Prognostication For Modern Therapy And Identifies Novel Therapeutic Targets, Ti'ara L Griffen, Fieke W Hoff, Yihua Qiu, James W Lillard, Alessandra Ferrajoli, Philip Thompson, Endurance Toro, Kevin Ruiz, Jan Burger, William Wierda, Steven M Kornblau Mar 2022

Proteomic Profiling Based Classification Of Cll Provides Prognostication For Modern Therapy And Identifies Novel Therapeutic Targets, Ti'ara L Griffen, Fieke W Hoff, Yihua Qiu, James W Lillard, Alessandra Ferrajoli, Philip Thompson, Endurance Toro, Kevin Ruiz, Jan Burger, William Wierda, Steven M Kornblau

Faculty, Staff and Student Publications

Protein expression for 384 total and post-translationally modified proteins was assessed in 871 CLL and MSBL patients and was integrated with clinical data to identify strategies for improving diagnostics and therapy, making this the largest CLL proteomics study to date. Proteomics identified six recurrent signatures that were highly prognostic of survival and time to first or second treatment at three levels: individual proteins, when grouped into 40 functionally related groups (PFGs), and systemically in signatures (SGs). A novel SG characterized by hairy cell leukemia like proteomics but poor therapy response was discovered. SG membership superseded other prognostic factors (Rai Staging, …

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The Use Of Atmospheric-Pressure Chemical Ionization For Pesticide Analysis Using Liquid Chromatography Mass Spectrometry, Pei-Ting Jiang, Kuan-Lu Wu, Hsueh-Ting Wang, Sung-Fang Chen Mar 2022

The Use Of Atmospheric-Pressure Chemical Ionization For Pesticide Analysis Using Liquid Chromatography Mass Spectrometry, Pei-Ting Jiang, Kuan-Lu Wu, Hsueh-Ting Wang, Sung-Fang Chen

Journal of Food and Drug Analysis

Based on the regulations of the Ministry of Health and Welfare (MOHW) of Taiwan in 2017, an analysis of 373 pesticides in food was conducted using the MOHW official method. The analyses involved the use of either liquid chromatography mass spectrometry (LC-MS) with electrospray ionization (ESI) or gas chromatography mass spectrometry (GC-MS) with electron ionization (EI). In this study, the applicability of detecting pesticides using atmospheric pressure chemical ionization (APCI) was investigated and evaluated. The pesticides were separated using an aqueous solution of ammonium formate with methanol as the mobile phase, and ionization efficiency was compared between APCI, ESI, and …

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A Substructure-Based Screening Approach To Uncover N-Nitrosamines In Drug Substances, Yu-Ting Kao, Shu-Fen Wang, Meng-Hsiu Wu, Shwu-Huey Her, Yi-Hsuan Yang, Chung-Hsien Lee, Hsiao-Feng Lee, An-Rong Lee, Li-Chien Chang, Li-Heng Pao Mar 2022

A Substructure-Based Screening Approach To Uncover N-Nitrosamines In Drug Substances, Yu-Ting Kao, Shu-Fen Wang, Meng-Hsiu Wu, Shwu-Huey Her, Yi-Hsuan Yang, Chung-Hsien Lee, Hsiao-Feng Lee, An-Rong Lee, Li-Chien Chang, Li-Heng Pao

Journal of Food and Drug Analysis

Drug substances are at risk of contamination with N-nitrosamines (NAs), well-known carcinogenic agents, during synthesis processes and/or long-term storage. Therefore, in this study, we developed an efficient data-based screening approach to systemically assess marketed products and investigated its scalability for benefiting both regulatory agencies and pharmaceutical industries. A substructure-based screening method employing DataWarrior, an open-source software, was established to evaluate the risks of NA impurities in drug substances. Eight NA substructures containing susceptible amino sources for N-nitrosation have been identified as screening targets: dimethylamine (DMA), diethylamine, isopropylethylamine, diisopropylamine, N-methyl-2-pyrrolidone, dibutylamine, methylphenylamine, and tetrazoles. Our method detected 192 drug …

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Repurposing Lansoprazole And Posaconazole To Treat Leishmaniasis: Integration Of In Vitro Testing, Pharmacological Corroboration, And Mechanisms Of Action, Yash Gupta, Steven Goicoechea, Jesus G. Romero, Raman Mathur, Thomas R. Caulfield, Daniel P. Becker, Ravi Durvasula, Prakasha Kempaiah Mar 2022

Repurposing Lansoprazole And Posaconazole To Treat Leishmaniasis: Integration Of In Vitro Testing, Pharmacological Corroboration, And Mechanisms Of Action, Yash Gupta, Steven Goicoechea, Jesus G. Romero, Raman Mathur, Thomas R. Caulfield, Daniel P. Becker, Ravi Durvasula, Prakasha Kempaiah

Journal of Food and Drug Analysis

Leishmaniasis remains a serious public health problem in many tropical regions of the world. Among neglected tropical diseases, the mortality rate of leishmaniasis is second only to malaria. All currently approved therapeutics have toxic side effects and face rapidly increasing resistance. To identify existing drugs with antileishmanial activity and predict the mechanism of action, we designed a drug-discovery pipeline utilizing both in-silico and in-vitro methods. First, we screened compounds from the Selleckchem Bio-Active Compound Library containing ~1,622 FDA-approved drugs and narrowed these down to 96 candidates based on data mining for possible anti-parasitic properties. Next, we completed preliminary in-vitro testing …

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