Life Sciences Commons^™

Interspecific And Ontogenic Differences In The Molar Occlusal Surfaces Of Manatees (Sirenia: Trichechidae), Lyle Goulbourne

Honors Theses

While much is known regarding limitless horizontal replacement of manatee teeth, the morphology and topography of the teeth have not been formally described, nor have interspecific differences and ontogenic changes in relative tooth size been described in detail. To physically describe the teeth, photographs of the molars from West Indian manatees (Trichechus manatus) and Amazonian manatees (T. inunguis) were taken and the features of the teeth were identified. To compare tooth size differences, the area of the posterior-most molar in occlusion was measured from both the upper and lower molars of the manatee skulls from West Indian and Amazonian manatees. …

Neuroprotective Effect Of Liraglutide In An Experimental Mouse Model Of Multiple Sclerosis: Role Of Ampk/Sirt1 Signaling And Nlrp3 Inflammasome, Reham A. Ammar, Ahmed F. Mohamed, Mohamed M. Kamal, Marwa M. Safar, Noha F. Abdelkader

Pharmacy

The heterogeneous nature of multiple sclerosis (MS) and the unavailability of treatments addressing its intricate network and reversing the disease state is yet an area that needs to be elucidated. Liraglutide, a glucagon-like peptide-1 analogue, recently exhibited intriguing potential neuroprotective effects. The currents study investigated its potential effect against mouse model of MS and the possible underlying mechanisms. Demyelination was induced in C57Bl/6 mice by cuprizone (400 mg/kg/day p.o.) for 5 weeks. Animals received either liraglutide (25 nmol/kg/day i.p.) or dorsomorphin, an AMPK inhibitor, (2.5 mg/ Kg i.p.) 30 min before the liraglutide dose, for 4 weeks (starting from the …

Quantitative Morphometric, Physiological, And Metabolic Characteristics Of Chickens And Mallards For Physiologically Based Kinetic Model Development, Colin G. Scanes, Johannes Witt, Stephan Schaller, Vanessa Baier, Audrey J. Bone, Thomas G. Preuss, David Heckmann

Poultry Science Faculty Publications and Presentations

Physiologically based kinetic (PBK) models are a promising tool for xenobiotic environmental risk assessment that could reduce animal testing by predicting in vivo exposure. PBK models for birds could further our understanding of species-specific sensitivities to xenobiotics, but would require species-specific parameterization. To this end, we summarize multiple major morphometric and physiological characteristics in chickens, particularly laying hens (Gallus gallus) and mallards (Anas platyrhynchos) in a meta-analysis of published data. Where such data did not exist, data are substituted from domesticated ducks (Anas platyrhynchos) and, in their absence, from chickens. The distribution of water …

Quantitative Comparison Of Avian And Mammalian Physiologies For Parameterization Of Physiologically Based Kinetic Models, Colin G. Scanes, Johannes Witt, Markus Ebeling, Stephan Schaller, Vanessa Baier, Audrey J. Bone, Thomas G. Preuss, David Heckmann

Poultry Science Faculty Publications and Presentations

Physiologically based kinetic (PBK) models facilitate chemical risk assessment by predicting in vivo exposure while reducing the need for animal testing. PBK models for mammals have seen significant progress, which has yet to be achieved for avian systems. Here, we quantitatively compare physiological, metabolic and anatomical characteristics between birds and mammals, with the aim of facilitating bird PBK model development. For some characteristics, there is considerable complementarity between avian and mammalian species with identical values for the following: blood hemoglobin and hemoglobin concentrations per unit erythrocyte volume together with relative weights of the liver, heart, and lungs. There are also …

Focused Review: The Role Of Drug Combinations For The Control Of Coccidiosis In Commercially Reared Chickens, H. David Chapman, Thilakar Rathinam

Poultry Science Faculty Publications and Presentations

A survey of drug combinations employed by the poultry industry indicates that they have played an important role in the control of coccidiosis in chickens. The mode of action of their component drugs is described. Advantages that accrue from their use may include a reduction in potential toxicity, a broadening of their spectrum of activity against different species of Eimeria, activity against different stages of the life cycle, and improved efficacy due to synergism between component drugs. Integration of management procedures involving rotation of drug combinations with vaccination is desirable because this has been shown to result in a restoration …

Avian Neuropeptide Y: Beyond Feed Intake Regulation, Elizabeth S. Greene, Nedra Abdelli, Jalila S. Dridi, Sami Dridi

Poultry Science Faculty Publications and Presentations

Neuropeptide Y (NPY) is one of the most abundant and ubiquitously expressed neuropeptides in both the central and peripheral nervous systems, and its regulatory effects on feed intake and appetite- have been extensively studied in a wide variety of animals, including mammalian and non-mammalian species. Indeed, NPY has been shown to be involved in the regulation of feed intake and energy homeostasis by exerting stimulatory effects on appetite and feeding behavior in several species including chickens, rabbits, rats and mouse. More recent studies have shown that this neuropeptide and its receptors are expressed in various peripheral tissues, including the thyroid, …

Strain-Specific Transcriptional Responses Overshadow Salinity Effects In A Marine Diatom Sampled Along The Baltic Sea Salinity Cline, Eveline Pinseel, Teofil Nakov, Koen Van Den Gerge, Kala M. Downey, Kathryn Judy, Olga Kourtchenko, Anke Kremp, Elizabeth C. Ruck, Conny Sjöqvist, Mats Töpel, Anna Godhe, Andrew J. Alverson

Biological Sciences Faculty Publications and Presentations

The salinity gradient separating marine and freshwater environments represents a major ecological divide for microbiota, yet the mechanisms by which marine microbes have adapted to and ultimately diversified in freshwater environments are poorly understood. Here, we take advantage of a natural evolutionary experiment: the colonization of the brackish Baltic Sea by the ancestrally marine diatom Skeletonema marinoi. To understand how diatoms respond to low salinity, we characterized transcriptomic responses of acclimated S. marinoi grown in a common garden. Our experiment included eight strains from source populations spanning the Baltic Sea salinity cline. Gene expression analysis revealed that low salinities …

Investigating The Role Of Fun30, A Chromatin Remodeler, In Dna Repair, Mehwish Iqbal

Dissertations

The repair of DNA double-strand breaks (DSBs) is crucial for maintaining genome stability. DSB repair needs to take place within the complex organization of the chromatin, and this requires changes in the chromatin structure adjacent to DSB sites. These changes occur through covalent histone modifications that alter histone-DNA contacts as well as by the action of ATP-dependent chromatin remodelers. Many chromatin remodelers, including Fun30, are involved in DSB repair. Fun30 facilitates DNA end resection at DSB site during the homologous recombination repair pathway. Apart from its role in DNA repair, Fun30 promotes gene silencing at heterochromatic loci such as telomeres, …

Yield, Nutrient Contents And Antioxidant Levels In Basil (Ocimum Basilicum L.) Grown In Aquaponic And Soil Systems, Maryam Ahmed Albedwawi

Theses

A study was conducted to evaluate the performance of Ocimum basilicum L. in both aquaponic and soil systems in the UAE climatic condition. The experiments were conducted under greenhouse conditions. Plants were raised in aquaponic beds with ornamental fishes in different concentrations. At the same time, a set of plants were raised in pots under a greenhouse set-up. The studied parameters were morphological (total weight and leaf number, shoot and root length, number of branches), biochemical (chlorophyll a, chlorophyll b, total chlorophyll, anthocyanin, xanthophyll, protein, and phenol contents) and antioxidant levels (Ascorbic acid and tocopherol). For analyzing the stress effects …

Elucidation Of The Interaction Between Hemorphins And Targets In The Renin-Angiotensin System, Amie Jobe

Theses

Hemorphins, short bioactive peptides produced by the enzymatic cleavage of the hemoglobin β-chain, exhibit anti-hypertensive effects via the inhibition of the angiotensin-1 converting enzyme (ACE1), a key component of the renin-angiotensin system (RAS) that governs blood pressure regulation. ACE1 and its homolog ACE2, which is also involved in the RAS, share significant similarity in their catalytic domains. The main objective of this work was to identify and compare the molecular mechanisms that underlie the interaction of hemorphins of camels and that of other mammals with ACE1 and ACE2. In silico docking and molecular dynamics simulations were employed, and in vitro …

Mineral Nutritional Status Of Plants Of A Public Park Under Long-Term Irrigation With Treated Sewage Effluent, Chandana Chakraborty

Theses

Treated sewage water is widely used for irrigation of urban greenery in UAE, and it contains significant number of elements that serve as nutrients for plants. The crucial unresolved question that remains, is regarding its long-term impact on the nutritional status of plants. Sulaimi Park of Al Ain was chosen as the study site. The main objective was to examine if the nutrient supply was adequate for plants. N, P, K levels were expected to exceed plant demands and micronutrient supply was assumed to be below plant requirements. To assess this, soil pH, annual nutrients input with water and elemental …

A Computational Assessment Of The Allosteric Modulation Of Gabaa Receptor, Abdel Rahman Atef Aly

Theses

γ-aminobutyric acid (GABA) type A receptor (GABA_AR) is a pentameric ligand gated ion channel and a member of the Cys-loop superfamily. It is targeted by several clinically significant allosteric modulators. The role of individual subunits of the receptor has been very extensively studied. However, the exact molecular pathway upon which the receptor is activated remains elusive. The main objective of this study was to probe the molecular activation pathway for the orthosteric GABA and to understand how two of the most well-established positive (PAM) and negative (NAM) allosteric modulators tend to modulate the receptor. Three independent long scale …

Standardisation Of Protocols Can Be Crucial In Long Non-Coding Rna Research, Kinga Németh, George A Calin

Faculty, Staff and Student Publications

In this issue, Traversa et al. [1] reviewed our current knowledge about the role of circular and linear forms of PVT1 non-coding RNA in cancer and human diseases. They highlighted the technical challenges of these studies and raised a potential bias in the publications, which require more attention from researchers.

Camk2/Camkii Activates Mlkl In Short-Term Starvation To Facilitate Autophagic Flux, Qionghui Zhan, Jaepyo Jeon, Ying Li, Yu Huang, Jian Xiong, Qiaochu Wang, Tian-Le Xu, Yong Li, Fu-Hai Ji, Guangwei Du, Michael X Zhu

Faculty, Staff and Student Publications

MLKL (mixed lineage kinase domain like pseudokinase) is a well-known core component of necrosome that executes necroptotic cell death upon phosphorylation by RIPK3 (receptor interacting serine/threonine kinase 3). Recent studies also implicate a role of MLKL in endosomal trafficking, which is not always dependent on RIPK3. Using mouse Neuro-2a and L929 as well as human HEK293 and HT29 cells, we show here that MLKL is phosphorylated in response to serum and amino acid deprivation from the culture medium, in a manner that depends on CAMK2/CaMKII (calcium/calmodulin dependent protein kinase II) but not RIPK3. The starvation-induced increase in MLKL phosphorylation was …

Inhibition Of Bcl2a1 By Stat5 Inactivation Overcomes Resistance To Targeted Therapies Of Flt3-Itd/D835 Mutant Aml, Kotoko Yamatani, Tomohiko Ai, Kaori Saito, Koya Suzuki, Atsushi Hori, Sonoko Kinjo, Kazuho Ikeo, Vivian Ruvolo, Weiguo Zhang, Po Yee Mak, Bogumil Kaczkowski, Hironori Harada, Kazuhiro Katayama, Yoshikazu Sugimoto, Jered Myslinski, Takashi Hato, Takashi Miida, Marina Konopleva, Yoshihide Hayashizaki, Bing Z Carter, Yoko Tabe, Michael Andreeff

Faculty, Staff and Student Publications

Tyrosine kinase inhibitors (TKIs) are established drugs in the therapy of FLT3-ITD mutated acute myeloid leukemia (AML). However, acquired mutations, such as D835 in the tyrosine kinase domain (FLT3-ITD/D835), can induce resistance to TKIs. A cap analysis gene expression (CAGE) technology revealed that the gene expression of BCL2A1 transcription start sites was increased in primary AML cells bearing FLT3-ITD/D835 compared to FLT3-ITD. Overexpression of BCL2A1 attenuated the sensitivity to quizartinib, a type II TKI, and venetoclax, a selective BCL2 inhibitor, in AML cell lines. However, a type I TKI, gilteritinib, inhibited the expression of BCL2A1 through inactivation of STAT5 and …

Development And Characterization Of Anti-Galectin-9 Antibodies That Protect T Cells From Galectin-9-Induced Cell Death, Riyao Yang, Linlin Sun, Ching-Fei Li, Yu-Han Wang, Weiya Xia, Boning Liu, Yu-Yi Chu, Laura Bover, Long Vien, Mien-Chie Hung

Faculty, Staff and Student Publications

Antibodies that target immune checkpoint proteins such as programmed cell death protein 1, programmed death ligand 1, and cytotoxic T-lymphocyte-associated antigen 4 in human cancers have achieved impressive clinical success; however, a significant proportion of patients fail to respond to these treatments. Galectin-9 (Gal-9), a β-galactoside-binding protein, has been shown to induce T-cell death and facilitate immunosuppression in the tumor microenvironment by binding to immunomodulatory receptors such as T-cell immunoglobulin and mucin domain-containing molecule 3 and the innate immune receptor dectin-1, suggesting that it may have potential as a target for cancer immunotherapy. Here, we report the development of two …

Measuring And Modelling Crop Yield Losses Due To Invasive Insect Pests Under Climate Change, Henri Ez Tonnang, Bonoukpoè M. Sokame, Elfatih M. Abdel-Rahman, Thomas Dubois

All Peer-Reviewed Publications

Climate change and agriculture are strongly correlated, and the fast pace of climate change will have impacts on agroecosystems and crop productivity. This review summarizes potential impacts of rising temperatures and atmospheric CO2 concentrations on insect pest-crop interactions and provides two-way approaches for integrating these impacts into crop models for sustainable pest management strategies designing. Rising temperatures and CO2 levels affect insect physiology, accelerate their metabolism and increase their consumption, ultimately increasing population densities, which result in greater crop injury and damage, and yield loss. Whereas these direct effects are empirically demonstrated for temperature rises, they are less straightforward for …

Impact Of Net Houses On The Natural Regulation Of The Populations Of Trialeurodes Vaporariorum (Homoptera: Aleyrodidae) And Tuta Absoluta (Lepidoptera: Gelechiidae), Two Major Tomato Pests In Kenya, Anaïs Chailleux, Junitor Chepkemoi, Julien M. Haran, Laure Benoit, Robert Copeland, Emilie Deletre

All Peer-Reviewed Publications

The abandonment of insecticide treatments to achieve sustainable crop production calls for a combination of methods to obtain satisfactory pest control. To this end, net houses and biological control are two promising methods, though we suspected that most natural enemies are blocked outside the net houses. In Kenya, tomato crops are particularly threatened by Trialeurodes vaporariorum (Westwood 1856) and Tuta absoluta (Meyrick, 1917) that are targets of most insecticide treatments in this country. We investigated in an on-farm experiment (i) the abundances of T. vaporariorum and T. absoluta in open fields and in net houses, (ii) the diversity of arthropod …

Organic Maize And Bean Farming Enhances Free-Living Nematode Dynamics In Sub-Saharan Africa, Janet G. Atandi, Noah Adamtey, Agnes W. Kiriga, Edward N. Karanja, Martha W. Musyoka, Felix M. Matheri, Chrysantus M. Tanga, Danny L. Coyne, Komi K.M. Fiaboe, David Bautze, Solveig Haukeland

All Peer-Reviewed Publications

Despite their important ecological roles for soil health and soil fertility, free-living nematodes (FLN) have received relatively limited research attention. The present study evaluated the community structure and diversity of FLN in a field setting. The experiments were conducted in on-farm and on-station field plots sown to maize (Zea mays) and beans (Phaseolus vulgaris) under four cropping practices. These farming systems included organic (compost and biopesticide use), conventional (synthetic fertilizer and pesticide applications), farmer practice (organic and synthetic amendments) and a control (non-amended plots). Nineteen genera of free living nematodes, belonging to bacterivores, fungivores, omnivores and predators were recorded. Among …

Species Diversity And Barcoding Of Macroinvertebrates From The San Antonio River, Gabrielle Silva

Student Research Symposium 2022 (Archived)

Macroinvertebrates are important biological indicators of health and ecological change within aquatic ecosystems. Macroinvertebrate diversity of the San Antonio (SA) River Watershed (Texas), which traverses rural and urban regions (Bexar County, TX), remains understudied compared to vertebrate counterparts of economic and conservation importance. The SA River hosts a diversity of crustaceans (crayfish), insects (beetles, dragonflies), annelids (leeches), and unidentified larvae at intermediate developmental stages. To improve ecological records of the SA River, an integrative approach is used to establish a reference macroinvertebrate species inventory and a genetic barcoding database (mitochondrial COI and/or 16S rRNA) for the SA River Mission Reach …

Cattle Diet Selection During The Growing Season On Upland Sandhills Rangelands, Alejandro Orozco-Lopez

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

The Sandhills is a diverse and complex ecosystem comprised of different topographic positions (i.e. slopes and interdunes) that influence grazing distribution, plant species composition, and cattle diet selection. The first objective of this study was to evaluate how grazing intensity influences species composition on slopes and interdunes on native rangeland within the Hillside pasture (160 ha) at the UNL Gudmundsen Sandhill’s Laboratory (GSL). The second objective of this study was to evaluate forage quality of individual species from three plant functional groups (i.e. warm-season grasses, cool-season grasses, and forbs/shrubs) on native rangeland within the Hillside pasture (160 ha) at GSL. …

Genomic Analysis Of Metabolic Differences Found In Clostridium Perfringens That Cause Necrotic Enteritis In Poultry, Connor M. Aylor

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

Clostridium perfringens is a common member of gut microbiota in healthy animals, but can also be an important pathogen in human and veterinary medicine. It produces several protein toxins that contribute to both histotoxic and enteric diseases in animals. Necrotic enteritis in poultry has been associated with the NetB toxin of C. perfringens; however, this toxin alone is insufficient to cause disease in infected chickens. While considerable research has focused on the presence of toxins and virulence factors, little has been done to assess the function of metabolic factors on the ability of the bacteria to cause disease. …

Fine Mapping And Cloning Of The Major Seed Protein Quantitative Trait Loci On Soybean Chromosome 20, Christina E. Fliege, Russell A. Ward, Pamela Vogel, Hanh Nguyen, Truyen Quach, Ming Guo, João Paulo Gomes Viana, Lucas Borges Dos Santos, James Specht, Thomas Clemente, Matthew E. Hudson, Brian W. Diers

Department of Agronomy and Horticulture: Faculty Publications

Soybean [Glycine max (L.) Merr.] is a unique crop species because it has high levels of both protein and oil in its seed. Of the many quantitative trait loci (QTL) controlling soybean seed protein content, alleles of the cqSeed protein-003 QTL on chromosome 20 exert the greatest additive effect. The high-protein allele exists in both cultivated and wild soybean (Glycine soja Siebold & Zucc.) germplasm. Our objective was to fine map this QTL to enable positional-based cloning of its underlying causative gene(s). Fine mapping was achieved by developing and testing a series of populations in which the chromosomal region surrounding …

Sex-Specific Effects Of Hatching Order On Nestling Baseline Corticosterone In A Wild Songbird, Madison A. Rittinger, Rachel M. Bowden, Logan A. Sauers, Ryan T. Paitz, Christine J. Poppe, Charles F. Thompson, Scott K. Sakaluk

Faculty Publications – Biological Sciences

Variation in nestling growth and survival is often influenced by hatching order, with first-hatched offspring having an advantage over later-hatched younger siblings. In house wrens (Troglodytes aedon), this effect of hatching order is especially evident in asynchronously hatched broods and can lead to sex-specific differences in the size and condition of nestlings. Females appear to allocate the sex of their offspring across the laying order to capitalize on these differences. We hypothesized that levels of circulating corticosterone, the primary metabolic hormone in birds, mediates these sex-specific effects in nestlings. We predicted that: i) baseline levels of corticosterone in nestlings should …

The Influence Of Particle Concentration And Bulk Characteristics On Polarized Oceanographic Lidar Measurements, Brian L. Collister, Richard C. Zimmerman, Charles I. Sukenik, William M. Balch, Victoria J. Hill

OES Faculty Publications

Oceanographic lidar measurements of the linear depolarization ratio, δ, contain information on the bulk characteristics of marine particles that could improve our ability to study ocean biogeochemistry. However, a scarcity of information on the polarized light-scattering properties of marine particles and the lack of a framework for separating single and multiple scattering effects on δ have hindered the development of polarization-based retrievals of bulk particle properties. To address these knowledge gaps, we made single scattering measurements of δ for several compositionally and morphologically distinct marine particle assemblages. We then used a bio-optical model to explore the influence of multiple …

Screening Commercial Entomopathogenic Fungi For The Management Of Diaphorina Citri Populations In The Lower Rio Grande Valley, Texas, Usa, Justin Wendel, Jonathan Cisneros, Stefan Jaronski, Christopher Vitek, Matthew Ciomperlik, Daniel Flores

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Ten strains of entomopathogenic ascomycete fungi, sourced from commercial formulations of blastopore or conidiospore formulations, were tested in 14 different formulations in a primary acquisition/direct spray bioassay against adult Asian citrus psyllid (Diaphorina citri Kuwayama (Hemiptera: Liviidae)). The Cordyceps (Isaria) javanica Apopka 97-C (conidia) strain was used as the standard. A statistical ranking system was established in which top performing pathogenic strains were selected for further screening and eventual field trials. Modified Potter-type spray towers were utilized to deliver a range of doses of viable spores to adult D. citri in an aqueous spray consistent with …

Cover Crops May Exacerbate Moisture Limitations On South Texas Dryland Farms, Stephanie Kasper, Faeqa Mohsin, Lindsey Richards, Alexis Racelis

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Cover crops are a sustainable management tool for mediating weed pressure, reducing soil erosion, and enhancing soil organic carbon (C) and nitrogen (N) levels. Yet, adoption rates across water-limited farms in Texas remain low, especially among producers without irrigation access, due to concerns that cover crop use of soil moisture will negatively impact subsequent cash crop yields. This three-year cover crop trial in a rain-fed sorghum (Sorghum bicolor L.) farm in Lyford, Texas, trialed different cover crop mixes and seeding rates and confirmed that cover cropping leads to significant soil moisture deficits and cash crop failure when rainfall is low …

Environment-Driven Shifts In Inter-Individual Variation And Phenotypic Integration Within Subnetworks Of The Mussel Transcriptome And Proteome, Richelle L. Tanner, Lani U. Gleason, W. Wesley Dowd

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

The environment can alter the magnitude of phenotypic variation among individuals, potentially influencing evolutionary trajectories. However, environmental influences on variation are complex and remain understudied. Populations in heterogeneous environments might exhibit more variation, the amount of variation could differ between benign and stressful conditions, and/or variation might manifest in different ways among stages of the gene-to-protein expression cascade or among physiological functions. Here, we explore these three issues by quantifying patterns of inter-individual variation in both transcript and protein expression levels among California mussels, Mytilus californianus Conrad. Mussels were exposed to five ecologically relevant treatments that varied in the mean …

1st Place Contest Entry: Designing Hollow Nanogels For Drug Delivery Applications, Mo Hijazi

Kevin and Tam Ross Undergraduate Research Prize

This is Mo Hijazi's submission for the 2022 Kevin and Tam Ross Undergraduate Research Prize, which won first place. It contains their essay on using library resources, their bibliography, and a summary of their research project on hollow-core nanogels.

Mo is a second-year student at Chapman University, majoring in Biological Sciences. Their faculty mentor is Dr. Molla Islam.

Luteolin Causes 5′Cpg Demethylation Of The Promoters Of Tsgs And Modulates The Aberrant Histone Modifications, Restoring The Expression Of Tsgs In Human Cancer Cells, Sreepoorna Pramodh, Ritu Raina, Arif Hussain, Sali Abubaker Bagabir, Shafiul Haque, Syed Tasleem Raza, Mohammad Rehan Ajamal, Shalini Behl, Deepika Bhagavatula

All Works

Cancer progression is linked to abnormal epigenetic alterations such as DNA methylation and histone modifications. Since epigenetic alterations, unlike genetic changes, are heritable and reversible, they have been considered as interesting targets for cancer prevention and therapy by dietary compounds such as luteolin. In this study, epigenetic modulatory behaviour of luteolin was analysed on HeLa cells. Various assays including colony forming and migration assays, followed by biochemical assays of epigenetic enzymes including DNA methyltransferase, histone methyl transferase, histone acetyl transferase, and histone deacetylases assays were performed. Furthermore, global DNA methylation and methylation‐specific PCR for examining the methylation status of CpG …