Life Sciences Commons^™

Genetic Diversity In Helianthus Annuus: Selective Breeding And Induced Mutagenesis, Dillon Holton

Thinking Matters Symposium

H. annuus, more commonly known as the sunflower, is a single species that has many different variations. Traits have been selectively bred for hundreds of years in order to produce a beautiful flower as well as essential agricultural products. Recently interest in the sunflower as a food and fuel crop has led to selective breeding of seeds that produce high yields of sunflower oil. To further push the limits of this plant's genome, researchers have used mutagenesis to force mutations in hopes of developing novel modifications that could increase yield. With the entire genome mapped, the goal for many research …

Classical Conditioning Of Cognitive States, Arthur Burns

Neuroscience Honors Papers

Classical conditioning has been a fundamental concept and practice throughout the history of psychology. While classical conditioning traditionally seeks to elicit target behaviors in correlation to specific stimuli, we sought to do the same with cognitive states in place of behaviors. Specifically, we wanted to determine the effectiveness of conditioning states of cognitive arousal in human participants in conjunction with cues presented in a designed learning task. We designed a cognitive task specifically for this research, referred to as “the Tone Pitching Task”, which utilized a combination of working memory and mental processing in order to elicit cognitive arousal and …

A Descriptive Pilot Study On Travel Nurses' Risk Of Burnout During The Covid- 19 Pandemic, Lashanda Head

ATU Theses and Dissertations 2021 - Present

Burnout is a state of emotional, physical, and mental exhaustion that results from long-term involvement in work situations that are difficult (Schaufeli & Greenglass, 2001). Burnout leads to a significant occupational problem in various professions that involve people who work with others, including nurses who work with COVID- 19 patients. This pilot study aims to determine if travel nurses who have worked or are currently working during the COVID-19 pandemic in acute care hospitals are at increased risk for nurse burnout. A convenience sample (N=9) of travel nurses working at CHRISTUS St Michael Health System were asked to …

Fighting Food Insecurity In New York City: What Role For Street Trees?, Kristen Cooney

Environment and Sustainability Honors Papers

There is growing recognition that urban forests have the potential to combat food insecurity via their edible parts, namely fruits, berries, and nuts. I researched New York City’s street trees, by locating trees with edible parts within food insecure communities, to analyze this potential.

The Various Nervous System Drugs Commonly Used For Treatment And The Disparities In Availability Of These Drugs In Minority Groups, Iman Haurami

Honors Scholars Collaborative Projects

The nervous system is a complex network in our body that is responsible for actions, reflexes, and even sensations. With the brain being part of the CNS, many common nervous system disorders are due to a defect in the central nervous system and the functions of this organ. These disorders include mental health problems, degenerative diseases, seizures, infections, and other possible genetic diseases. Many of these diseases are common in society and constantly researched. Even though these diseases are common, treatment accessibility and effects on people of minority groups are overlooked compared to the focus on majority groups. For drug …

Développement D’Une Méthodologie Pour Évaluer La Performance Des Gants À Isoler Des Vibrations En Tenant Compte De La Dextérité Ainsi Que De La Force De Préhension, Pierre Marcotte, Subhash Rakheja, Christian Larivière, Yumeng Yao, Chantal Gauvin, Karim Hamouda, Hakim Mecheri

Rapports de recherche scientifique

L'efficacité des gants antivibrations pour réduire les vibrations transmises à la main est actuellement évaluée en laboratoire selon la méthodologie décrite dans la norme ISO 10819:2013. Cette norme évalue la transmission des vibrations à la paume de la main, mais ne tient pas compte de la transmission des vibrations aux doigts ni de l’effet du port de ces gants sur la dextérité manuelle et l’effort de préhension. Cette étude visait à développer une méthodologie afin d’améliorer l’évaluation de la transmission des vibrations par les gants antivibrations à la paume et aux doigts, tout en tenant compte de l’effet de ces …

A Role For Myosin Ii Clusters And Membrane Energy In Cortex Rupture For Dictyostelium Discoideum, Emmanuel Asante-Asamani, Daniel Grange, Devarshi Rawal, Zully Santiago, John Loustau, Derrick Brazill

Publications and Research

Blebs, pressure driven protrusions of the cell membrane, facilitate the movement of eukaryotic cells such as the soil amoeba Dictyostelium discoideum , white blood cells and cancer cells. Blebs initiate when the cell membrane separates from the underlying cortex. A local rupture of the cortex, has been suggested as a mechanism by which blebs are initiated. However, much clarity is still needed about how cells inherently regulate rupture of the cor- tex in locations where blebs are expected to form. In this work, we examine the role of mem- brane energy and the motor protein myosin II (myosin) in facilitating …

A Spatial Assessment Of Impacts To The Flats Fishery By Recreational Boating In The Florida Keys National Marine Sanctuary, Kristin Anderson

All HCAS Student Capstones, Theses, and Dissertations

The recreational flats fishery in the Florida Keys is a significant component of the marine resource-based economy, exceeding $465 million (USD) in annual economic impact. Permit (Trachinotus falcatus), bonefish (Albula vulpes), and tarpon (Megalops atlanticus) are the three main species targeted by flat fishers. Those participating in this fishery generally practice catch-and-release angling therefore, the fishery is considered a more sustainable marine use compared to more traditional commercial fisheries. However, with population and tourism rising in South Florida, the fishery is increasingly threatened by habitat degradation and user conflicts. Ongoing revisions to the Florida …

Covid-19 Impact On The Hospitalization Rates For Eating Disorders, Jennifer Ess

Honors Theses

The COVID-19 pandemic has had an impact in a variety of ways. With the quarantine restrictions placed during this time, many individuals had turned to social media as an outlet to cope with the loss of intimate time with friends and outside family. This increase in social media use, especially Tik Tok and Instagram, can negatively impact a person's outlook on certain situations. Throughout this pandemic, mental health issues, specifically eating disorders, have shown a rise in cases during this time. Current research shows that numbers have more than doubled during pandemic times, and also shows many different factors that …

P-Flux: A Phosphorus Budget Dataset Spanning Diverse Agricultural Production Systems In The United States And Canada, M. R. Williams, P. Welikhe, J. Bos, K. King, M. Akland, D. J. Augustine, C. Baffaut, E. G. Beck, A. Bierer, D. D. Bosch, Elizabeth H. Boughton, C. B. Brandani, E. Brooks, Anthony R. Buda, M. Cavigelli, J. Faulkner, G. Feyereisen, A. Fortunato, B. Lee, Jason M. Unrine

Plant and Soil Sciences Faculty Publications

No abstract provided.

Indispensable Role Of Immunophenotyping In Diagnosing Leukemic Phase Of Blastic Plasmacytoid Dendritic Cell Neoplasm Without Cutaneous Manifestation, Hareem Alam, Nabiha Saeed, Anila Rashid

Department of Pathology and Laboratory Medicine

Blastic plasmacytoid dendritic cell neoplasm usually presents as skin lesions. Diagnostic error occurs when it primarily presents in leukemic phase without skin involvement. Triad of CD4, CD56 and CD123 immunophenotype expression is essential to avoid misdiagnosis of this rare hematological malignancy. Here we describe a patient who presented in overt leukemic phase of BPDCN highlighting diagnostic challenges encountered that resulted in delayed diagnosis and poor outcome

Targeting Platelets Using Ionic Liquid Coated Nanoparticles, Karen Mun Wong

Honors Theses

Platelets are key players in our body’s inflammatory processes upon activation, which can create physiological problems. Researchers are actively seeking solutions by targeting platelets with therapeutics. The objective of the research in this thesis was to investigate a new and potentially less invasive drug delivery platform using nanoparticles. The topic of the project was to explore the world of ionic liquids and their abilities, when coated on nanoparticles, to bind to platelets. A library of choline carboxylic acid-based ionic liquids (ILs) was first synthesized via salt metathesis while PLGA-based nanoparticles were simultaneously being synthesized via the nanoprecipitation method. The ionic …

Examining Bacteriophage Reliance On The Tola Protein Of Escherichia Coli, Ellen Davis

Honors Projects

With the ever-growing crisis of antibiotic resistant bacteria, bacteriophage provide an important potential alternative therapy to treat infections when antibiotics are ineffective. For this reason, it is important to understand the mechanisms bacteriophage use to enter their bacterial host cell. The cellular envelope of the gram-negative bacterium E. coli contains a protein system known as Tol-Pal, which utilizes energy from the Proton Motive Force to carry out several cellular functions, including some associated with cellular division and outer-membrane stability. Certain bacteriophage take advantage of the energy produced by this system to cross the bacterial cell membrane. One important protein of …

Exploring The Relationship Between Art And Environmental Education, Mackenzie Haynes

Honors Projects

To explore the relationship between art and environmental education, I created a lesson plan and then put into practice at Crim Elementary School in a 4th grade art class. The art project had to do with the environment, endangered animals and recyclable materials. I titled the project "Habitat Heroes" and students had to imagine that they were the only people left on Earth along with a few animals and lots of trash. They were tasked with selecting an endangered animal in Ohio from one of five different groups (mammals, birds, reptiles/amphibians, birds and fish) to create a habitat for. The …

Mechanobiology In The Comorbidities Of Ehlers Danlos Syndrome, Shaina P. Royer, Sangyoon J. Han

Michigan Tech Publications, Part 1

Ehlers-Danlos Syndromes (EDSs) are a group of connective tissue disorders, characterized by skin stretchability, joint hypermobility and instability. Mechanically, various tissues from EDS patients exhibit lowered elastic modulus and lowered ultimate strength. This change in mechanics has been associated with EDS symptoms. However, recent evidence points toward a possibility that the comorbidities of EDS could be also associated with reduced tissue stiffness. In this review, we focus on mast cell activation syndrome and impaired wound healing, comorbidities associated with the classical type (cEDS) and the hypermobile type (hEDS), respectively, and discuss potential mechanobiological pathways involved in the comorbidities.

Individual Trophic Niche Specialization In American Beaver (Castor Canadensis), Jimmy Taylor, Robert Francis, Scott Rush, Bronson Strickland, Guiming Wang

United States Department of Agriculture Wildlife Services: Staff Publications

The American beaver (Castor canadensis) has been described as a choosy generalist at the species/population scale, yet observational studies have shown little variation in diet among individuals. We compared isotopic values of δ¹³C or δ¹⁵N taken from hair of 32 beaver, representing seven colonies in northern Alabama, USA to determine 1) if colonies of beaver show overlap in isotopic niche width as a result of the similar use of food resources and 2) if individual trophic niche specialization occurs within colonies. Total Trophic Niche Width varied across the wetland with the widest being twice …

A Psychopharmacology Review Of Brexpiprazole, Nicole A. Marion

Conspectus Borealis

Brexpiprazole is an antipsychotic drug often used to help treat schizophrenia and major depressive disorder. This review paper breaks down the drugs history including what it is used for, how it is used, and how it was developed. Pharmacokinetics including administration routes, steady states, and metabolism. This review also describes pharmacodynamics, focusing on drug affinity. It will also discuss pharmacological effects pulling evidence from clinical trials. Last this review will describe the toxicology and safety of brexpiprazole.

Effects Of Seasonal Variation Of Abiotic Factors On Edna Detection Of Two Groundwater Crayfish Species, Jared Prine Higgs

Honors Capstone Projects and Theses

No abstract provided.

Pickle Associates With Histone Deacetylase 9 To Mediate Vegetative Phase Change In Arabidopsis, Tieqiang Hu, Darren Manuela, Valerie Hinsch, Mingli Xu

Faculty Publications

• The juvenile-to-adult vegetative phase change in flowering plants is mediated by a decrease in miR156 levels. Downregulation of MIR156A/MIR156C, the two major sources of miR156, is accompanied by a decrease in acetylation of histone 3 lysine 27 (H3K27ac) and an increase in trimethylation of H3K27 (H3K27me3) at MIR156A/MIR156C in Arabidopsis.

• Here, we show that histone deacetylase 9 (HDA9) is recruited to MIR156A/MIR156C during the juvenile phase and associates with the CHD3 chromatin remodeler PICKLE (PKL) to erase H3K27ac at MIR156A/MIR156C.

• H2Aub and H3K27me3 become enriched at MIR156A/MIR156C, and the recruitment of Polycomb Repressive Complex …

Does A Hypertrophying Muscle Fibre Reprogramme Its Metabolism Similar To A Cancer Cell?, Henning Wackerhage, Ivan J. Vechetti, Philipp Baumert, Sebastian Gehlert, Lore Becker, Richard T. Jaspers, Martin Hrabě De Angelis

Department of Nutrition and Health Sciences: Faculty Publications

In 1924, Otto Warburg asked “How does the metabolism of a growing tissue differ from that of a non-growing tissue?” Currently, we know that proliferating healthy and cancer cells reprogramme their metabolism. This typically includes increased glucose uptake, glycolytic flux and lactate synthesis. A key function of this reprogramming is to channel glycolytic intermediates and other metabolites into anabolic reactions such as nucleotide-RNA/DNA synthesis, amino acid-protein synthesis and the synthesis of, for example, acetyl and methyl groups for epigenetic modification. In this review, we discuss evidence that a hypertrophying muscle similarly takes up more glucose and reprogrammes its metabolism to …

Exchange Protein Directly Activated By Camp (Epac1) Protects Human Endothelial Cells From Tumor Necrosis Factor-⍺ (Tnf-⍺) Induced Cell Death, Anh Luu, Abigail Carpenter, Rosetta Tolley

ONU Student Research Colloquium

Millions of people are affected by diseases involving inflammatory states such as sepsis. Sepsis is associated with elevated tumor necrosis factor alpha (TNF-⍺) which mediates inflammation. Excessive TNF-⍺ contributes to death of endothelial cells (EC) with subsequent disruption of vascular endothelial function. Exchange Factor Directly Activated by cAMP (EPAC1) is a cyclic-AMP (cAMP) sensor that regulates multiple EC functions. We hypothesized that EPAC1 activation affects the response of EC to TNF-a. Microvascular EC were pretreated for 45 minutes with 100 mM 8-pCPT-2′-O-Me-cAMP (8-CPT, specific EPAC1 activator) or 10 mM forskolin (adenylyl cyclase activator that increases cAMP), then exposed to 5 …

Genetic Variation In The Ohio Population Of Tonguetied Minnow (Exoglossum Laurae), Emmily Moses, Sarah Armstrong, Madeline Williams

ONU Student Research Colloquium

A singular population of tonguetied minnow (Exoglossum laurae) inhabits a ~50-km segment of the Mad River of western Ohio. Tonguetied minnow is Endangered in Ohio as a consequence of its small geographic range and low abundance. For this study, the polymerase chain reaction (PCR) was used amplify five genetic loci from 35 tonguetied minnow captured from the Mad River. Loci included two mitochondrial genes, the NADH-dehydrogenase 2 (ND2) and the control region (D-loop). From the nuclear genome, myosin heavy chain 6 (myh6) and β-actin (β-act) introns were amplified, as well as a portion of the major histocompatibility (Mhc-IIB) …

The Effect Of Physiographic Regions On Benthic Riffle Communities In The Kokosing River (Ohio), Cameron Brisbine, Gregory Brown, Jack Kessler

ONU Student Research Colloquium

Geology, physiographic history, and land use are among the most important factors influencing benthic community structure and distribution in surface waters, especially lotic systems. Given the higher till concentrations in glaciated regions, does this impact the quality of riffle habitats and associated benthic communities? The objective of this study was to examine the influence of glacial history on benthic stream communities and physicochemical variables of a river in central Ohio. The Kokosing River transitions through several physiographic regions within Ohio including the Till Plains, the Glaciated Allegheny Plateau, and the Unglaciated Allegheny Plateau. From 2020-2021, periphyton, macroinvertebrates, fish, and selected …

Using Integrated Rate Equations To Analyze Acid Phosphatase Kinetics, Melissa Gouge

ONU Student Research Colloquium

In an academic setting, enzyme kinetics are introduced by analysis of the initial rates using the Michaelis-Menten model. To better understand enzyme kinetics, a complete time course analysis will provide a larger set of data, including information on feedback or product inhibition. With this, the Lambert W function can be used to analyze the integrated rate equations. We have started our analysis with the enzyme, acid phosphatase. Inorganic phosphorus is a known competitive inhibitor of the acid phosphatase catalyzed hydrolysis of p-nitrophenylphosphate. Using the integrated form of the rate equation allows us to determine the relative relationship of the K …

Study Proposal For Additional Investigation Of Epac1 In Endothelial Apoptosis, Anh Luu

ONU Student Research Colloquium

Inflammatory states such as sepsis are associated with elevated tumor necrosis factor alpha (TNF-⍺), which facilitates inflammation, increases the production of reactive oxygen species (ROS) and oxidative stress, and contributes to death of endothelial cells (EC). Exchange Factor Directly Activated by cAMP (EPAC1) is an intracellular cyclic-AMP (cAMP) sensor involved in EC responses such as barrier function, angiogenesis and proliferation. Previous studies in our lab demonstrated activation of EPAC1 protected EC from TNF⍺-induced cell death. Specifically, microvascular EC were pretreated for 45 minutes with 100 mM 8-pCPT-2′-O-Me-cAMP (8CPT, specific EPAC1 activator) or 10 mM forskolin (adenylyl cyclase activator that increases …

Chromatin Architecture: Mechanisms Of Gene Regulation, Logan O'Donnell

Honors Projects in Science and Technology

The rapid growth and division of cells as they proliferate and penetrate to surrounding tissues defines the collection of disease states known as cancer. Abnormal gene expression drives this uncontrollable replication of cells. In recent studies, aberrant HOX gene expression has been noted in a multitude of cancer types such as myeloid leukemia, prostate cancer, and breast cancer [1]. Within cancerous cells with aberrant HOX expression, late expressing HOX genes are suppressed while early expressing HOX genes are reactivated. HOX genes are significant in controlling early phases of organismal development such as cell cycle, cell movement, and gene expression. Likewise, …

Non-Native Coccinellid Beetles And Land Use Abundance Patterns In The Quad Cities Region, Jack Hughes, Tierney Brosius, Michael Reisner

Biology: Student Scholarship & Creative Works

Harmonia axyridis was introduced in 1916. This species of coccinellid beetle is considered a desirable species for agriculture practices because of its ability to control pests. There is increasing evidence that H. axyridis may be outcompeting native species which could be contributing to the decline of some species that are disappearing from the landscape. Harmonia axyridis has a large migration range of 2 Km enabling the species to affect a large area of land. Invasive species often increase in abundance in urban landscapes which may be the case with H. axyridis. During the summer of 2021 we investigated H. axyridis …

The Impact Of Supplemental Proline On Stress Tolerance In Cucumbers And Tomatoes, Wren Allison Jenkins

Honors Capstone Projects and Theses

No abstract provided.

Effects Of Urbanization On House Sparrow (Passer Domesticus) And House Finch (Haemorhous Mexicanus) Song Propagation, Sarah Grimes, Eliza Lewis

21st Annual Celebration of Undergraduate Research and Creative Activity (2022)

The recent surge in urbanization has increased pollution, which includes both physical pollution (e.g. exhaust) and sensory pollution (e.g., anthropogenic noise). We know birds increase the frequency and amplitude of their song in urban areas to reduce masking by low frequency noise pollution. However, bird song (i.e., a signal) is also affected by the environment and accompanying ambient noise. This study investigates how anthropogenic disturbances alter the ability of birds to communicate. Specifically, we aim to understand how urbanization affects the propagation of bird song by examining differences in active space, or the maximum distance a receiver can detect a …

Changes In Chromatic Contrast Of Avian Plumage In Forests With Different Levels Of Deer Browsing, Morgan Sherrard, Suihnem Mawi, Timothy Boycott, Megan Gall

21st Annual Celebration of Undergraduate Research and Creative Activity (2022)

Visual signal propagation through the environment can be influenced by many factors, including the visual background. For example, normally camouflaged animals can appear quite salient when the environmental substrate is changed. Deer can alter this visual background by consuming the forest understory; ultimately this can have implications for species that use visual signals to attract a mate or defend a territory. We are interested in studying how deer browsing affects the chromatic contrast (i.e., how much an animal stands out from the background for a given receiver) using an avian model system. Brown headed cowbirds (Molothrus ater) and wild turkeys …