Life Sciences Commons^™

Smyd3 Impedes Small Cell Lung Cancer Sensitivity To Alkylation Damage Through Rnf113a Methylation-Phosphorylation Cross-Talk, Valentina Lukinović, Simone Hausmann, Gael S Roth, Clement Oyeniran, Tanveer Ahmad, Joshua R Brickner, Florent Chuffart, Ana Morales Benitez, Jessica Vayr, Rebecca Rodell, Marianne Tardif, Pascal W T C Jansen, Yohann Couté, Michiel Vermeulen, Pierre Hainaut, Pawel K Mazur, Nima Mosammaparast, Nicolas Reynoird

Faculty, Staff and Student Publications

Small cell lung cancer (SCLC) is the most fatal form of lung cancer, with dismal survival, limited therapeutic options, and rapid development of chemoresistance. We identified the lysine methyltransferase SMYD3 as a major regulator of SCLC sensitivity to alkylation-based chemotherapy. RNF113A methylation by SMYD3 impairs its interaction with the phosphatase PP4, controlling its phosphorylation levels. This cross-talk between posttranslational modifications acts as a key switch in promoting and maintaining RNF113A E3 ligase activity, essential for its role in alkylation damage response. In turn, SMYD3 inhibition restores SCLC vulnerability to alkylating chemotherapy. Our study sheds light on a novel role of …

Does A Multiple Myeloma Polygenic Risk Score Predict Overall Survival Of Patients With Myeloma?, Angelica Macauda, Alyssa Clay-Gilmour, Thomas Hielscher, Michelle A T Hildebrandt, Marcin Kruszewski, Shaji K Kumar, Enrico Orciuolo, Elizabeth E Brown, Asta Försti, Rosalie G Waller, Mitchell J Machiela, Stephen J Chanock, Nicola J Camp, Marcin Rymko, Małgorzata Raźny, Wendy Cozen, Judit Várkonyi, Chiara Piredda, Matteo Pelosini, Alem A Belachew, Edyta Subocz, Kari Hemminki, Malwina Rybicka-Ramos, Graham G Giles, Roger L Milne, Jonathan N Hofmann, Jan Maciej Zaucha, Annette Juul Vangsted, Hartmut Goldschmidt, S Vincent Rajkumar, Waldemar Tomczak, Juan Sainz, Aleksandra Butrym, Marzena Watek, Elżbieta Iskierka-Jazdzewska, Gabriele Buda, Dennis P Robinson, Artur Jurczyszyn, Marek Dudziński, Joaquin Martinez-Lopez, Jason P Sinnwell, Susan L Slager, Krzysztof Jamroziak, Rui Manuel Vieira Reis, Niels Weinhold, Parveen Bhatti, Luis G Carvajal-Carmona, Daria Zawirska, Aaron D Norman, Grzegorz Mazur, Sonja I Berndt, Daniele Campa, Celine M Vachon, Federico Canzian

Faculty, Staff and Student Publications

Background: Genome-wide association studies (GWAS) of multiple myeloma in populations of European ancestry (EA) identified and confirmed 24 susceptibility loci. For other cancers (e.g., colorectum and melanoma), risk loci have also been associated with patient survival.

Methods: We explored the possible association of all the known risk variants and their polygenic risk score (PRS) with multiple myeloma overall survival (OS) in multiple populations of EA [the International Multiple Myeloma rESEarch (IMMEnSE) consortium, the International Lymphoma Epidemiology consortium, CoMMpass, and the German GWAS] for a total of 3,748 multiple myeloma cases. Cox proportional hazards regression was used to assess the association …

The Emerging Spectrum Of Cardiopulmonary Pathology Of The Coronavirus Disease 2019 (Covid-19): Report Of 3 Autopsies From Houston, Texas, And Review Of Autopsy Findings From Other United States Cities, Matthew S Walters

Faculty, Staff and Student Publications

The mammalian respiratory system or lung is a tree-like branching structure, and the main site of gas exchange with the external environment. Structurally, the lung is broadly classified into the proximal (or conducting) airways and the distal alveolar region, where the gas exchange occurs. In parallel with the respiratory tree, the pulmonary vasculature starts with large pulmonary arteries that subdivide rapidly ending in capillaries adjacent to alveolar structures to enable gas exchange. The NOTCH signalling pathway plays an important role in lung development, differentiation and regeneration post-injury. Signalling via the NOTCH pathway is mediated through activation of four NOTCH receptors …

Pramlintide: A Novel Therapeutic Approach For Osteosarcoma Through Metabolic Reprogramming, Yuanzheng Yang, Zhanglong Peng, Elsa R Flores, Eugenie S Kleinerman

Faculty, Staff and Student Publications

Despite aggressive combination chemotherapy and surgery, outcomes for patients with osteosarcoma have remained stagnant for more than 25 years, and numerous clinical trials have identified no new therapies. p53 deletion or mutation is found in more than 80% of osteosarcoma tumors. In p53-deficient cancers with structurally altered p63 and p73, interfering with tumor cell metabolism using Pramlintide (an FDA-approved drug for type 2 diabetes) results in tumor regression. Pramlintide response is mediated through upregulation of islet amyloid polypeptide (IAPP). Here, we showed that osteosarcoma cells have altered p63, p73, and p53, and decreased IAPP expression but have the two main …

Association Study Between Polymorphisms In Dna Methylation-Related Genes And Testicular Germ Cell Tumor Risk, Chiara Grasso, Maja Popovic, Elena Isaevska, Fulvio Lazzarato, Valentina Fiano, John Pluta, Kurt D'Andrea, Kristian Almstrup, Lynn Anson-Cartwright, D Timothy Bishop, Stephen J Chanock, Chu Chen, Victoria K Cortessis, Marlene D Dalgaard, Siamak Daneshmand, Alberto Ferlin, Carlo Foresta, Megan N Frone, Marija Gamulin, Jourik A Gietema, Mark H Greene, Tom Grotmol, Robert J Hamilton, Trine B Haugen, Russ Hauser, Robert Karlsson, Lambertus A Kiemeney, Davor Lessel, Patrizia Lista, Ragnhild A Lothe, Chey Loveday, Coby Meijer, Kevin T Nead, Jérémie Nsengimana, Rolf I Skotheim, Clare Turnbull, David J Vaughn, Fredrik Wiklund, Tongzhang Zheng, Andrea Zitella, Stephen M Schwartz, Katherine A Mcglynn, Peter A Kanetsky, Katherine L Nathanson, Lorenzo Richiardi

Faculty, Staff and Student Publications

Background: Testicular germ cell tumors (TGCT), histologically classified as seminomas and nonseminomas, are believed to arise from primordial gonocytes, with the maturation process blocked when they are subjected to DNA methylation reprogramming. SNPs in DNA methylation machinery and folate-dependent one-carbon metabolism genes have been postulated to influence the proper establishment of DNA methylation.

Methods: In this pathway-focused investigation, we evaluated the association between 273 selected tag SNPs from 28 DNA methylation-related genes and TGCT risk. We carried out association analysis at individual SNP and gene-based level using summary statistics from the Genome Wide Association Study meta-analysis recently conducted by the …

Biomolecular Condensation: A New Phase In Cancer Research, David J H Shih, Kara M Cirillo, Sueda H Cetinkaya, Wenjin Jim Zheng, Gordon B Mills, S Stephen Yi, Daniel F Jarosz, Nidhi Sahni

Faculty, Staff and Student Publications

Multicellularity was a watershed development in evolution. However, it also meant that individual cells could escape regulatory mechanisms that restrict proliferation at a severe cost to the organism: cancer. From the standpoint of cellular organization, evolutionary complexity scales to organize different molecules within the intracellular milieu. The recent realization that many biomolecules can "phase-separate" into membraneless organelles, reorganizing cellular biochemistry in space and time, has led to an explosion of research activity in this area. In this review, we explore mechanistic connections between phase separation and cancer-associated processes and emerging examples of how these become deranged in malignancy.

SIGNIFICANCE: One …

The Federated Circles Of The Mandarin Garden Club Year Book 1958, Mandarin Community Club

Mandarin Garden Club Yearbooks

Mandarin Garden Club yearbook listing committee members, activities, meetings and member rosters. Edited by Mrs. John B. Ross

The Federated Circles Of The Mandarin Garden Club Year Book 1960, Mandarin Community Club

Mandarin Garden Club Yearbooks

Mandarin Garden Club yearbook listing committee members, activities, meetings and member rosters. Edited by Mrs. George Lee Knecht.

The Federated Circles Of The Mandarin Garden Club Year Book 1957, Mandarin Community Club

Mandarin Garden Club Yearbooks

Mandarin Garden Club yearbook listing committee members, activities, meetings and member rosters. Edited by Mrs. Hans Taenzler

Type Of Stress Induces Differential Responses In Acer Rubrum (Red Maple), But Induced Responses Have No Effect On Herbivorous Pests, Cindy Perkovich, Anthony Witcher, Grayson Delay, Karla Addesso

Agricultural and Environmental Sciences Faculty Research

Plants thrive in dynamic environments requiring adaptive strategies in response to environmental stressors. Furthermore, insect herbivores may be attracted or deterred by the expression of these traits. This study examines growth, physiological, and phytochemical adaptations of maple trees in response to stressors and how these stressors effect herbivore feeding behavior within an agricultural production system. Agricultural systems are unique because plants experience environmental stressors unique to production such as herbicide sprays and girdling. Using four environmental stressors commonly observed in agricultural production (control, mechanical defoliation, chemical defoliation, and girdling), applied to two cultivars of red maple (Acer rubrum, ‘Brandywine’ and …

Circadian Clock Controls Rhythms In Ketogenesis By Interfering With Ppar Alpha Transcriptional Network, Volha Mezhnina, Oghogho P. Ebeigbe, Nikkhil Velingkaar, Allan Poe, Yana I. Sandlers, Roman Kondratov

Biological, Geological, and Environmental Faculty Publications

Ketone bodies are energy-rich metabolites and signaling molecules whose production is mainly regulated by diet. Caloric restriction (CR) is a dietary intervention that improves metabolism and extends longevity across the taxa. We found that CR induced high -amplitude daily rhythms in blood ketone bodies (beta-hydroxybutyrate [beta OHB]) that correlated with liver beta OHB level. Time-restricted feeding, another periodic fasting-based diet, also led to rhythmic beta OHB but with reduced amplitude. CR induced strong circadian rhythms in the expression of fatty acid oxidation and ketogenesis genes in the liver. The transcriptional factor peroxisome-proliferator-activated-receptor alpha (PPAR alpha) and its transcriptional target hepatokine …

Screening And Assessment Of Cancer-Related Fatigue: An Executive Summary And Road Map For Clinical Implementation, Joy C. Cohn, Shana Harrington, Jeannette Lee, Daniel Malone, Mary I. Fisher

Physical Therapy Faculty Publications

Background: Cancer-related fatigue (CRF) prevalence is reported as high as 90%. Cancer-related fatigue is multidimensional and associated with lower health-related quality of life. Effective screening and assessment are dependent upon use of valid, reliable, and clinically feasible measures. This Executive Summary of the Screening and Assessment of Cancer-related Fatigue Clinical Practice Guideline provides recommendations for best measures to screen and assess for CRF based on the quality and level of evidence, psychometric strength of the tools, and clinical utility.

Methods: After a systematic review of the literature, studies evaluating CRF measurement tools were assessed for quality; data extraction included psychometrics …

Responses Of Soil Heterotrophic Respiration And Microbial Biomass To Organic And Conventional Production Systems, Kripa Dhakal, Madhav Parajuli, Siyang Jian, Jianwei Li, Dilip Nandwani

Agricultural and Environmental Sciences Faculty Research

The effects of organic and conventional production systems on crop productivity have been greatly explored, but their effects on soil microbial processes were often neglected. A comparative field study of organic and conventional production systems was conducted at the Tennessee State University research farm to determine soil heterotrophic respiration and microbial biomass carbon. Leafy green vegetables were grown in a conventional production system in an open field, and they were grown in an organic production system, using three different row covers (agribon cloth, insect net, and plastic), and in an open field. Soil samples (0-15cm) were collected from the two …

The Cryptococcus Neoformans Flc1 Homologue Controls Calcium Homeostasis And Confers Fungal Pathogenicity In The Infected Hosts, Piotr R. Stempinski, Kristie D. Goughenour, Lukas M. Du Plooy, J. Andrew Alspaugh, Michal A. Olszewski, Lukasz Kozubowski

Open Access Publishing Fund

Cryptococcus neoformans, an opportunistic yeast pathogen, relies on a complex network of stress response pathways that allow for proliferation in the host. In Saccharomyces cerevisiae, stress responses are regulated by integral membrane proteins containing a transient receptor potential (TRP) domain, including the flavin carrier protein 1 (Flc1), which regulates calcium homeostasis and flavin transport. Here, we report that deletion of C. neoformans FLC1 results in cytosolic calcium elevation and increased nuclear content of calcineurin-dependent transcription factor Crz1, which is associated with an aberrant cell wall chitin overaccumulation observed in the flc1Δ mutant. Absence of Flc1 or inhibition …

Monarch Science Observer, Volume 15, College Of Sciences, Old Dominion University

College of Sciences Newsletter

Fall 2022, issue of Monarch Science Observer, ODU Colleges of Sciences Newsletter.

Post-Fire Erosion Following The Czu Lightning Complex Fire: Quantifying Hillslope Erosion And Providing Guidance Towards Improving Post-Fire Response, Matthew C. Crockett, Matthew Crockett

Master of Science in Environmental Sciences and Management Projects

The size and severity of wildfires have increased in California during recent decades. This trend is highlighted through the CZU Lightning Complex fire of August 2020 which burned over 86,000 acres in the Santa Cruz mountains of California. The fire greatly impacted the Little Creek watershed, a roughly 1,300 acre watershed that exists largely within Cal Poly San Luis Obispo’s Swanton Pacific Ranch (SPR). The current trends of California’s increased wildfire regime are expected to continue, raising concerns regarding the direct and secondary effects on forest watersheds and the effectiveness of current post-fire erosion control management. Accelerated rates of erosion …

Vermicompost Versus Traditional Compost Amendments Leads To Different Soil Health Outcomes In A Mediterranean Vineyard, Elizabeth L. Luck

Master of Science in Environmental Sciences and Management Projects

Compost application has become a common practice in vineyard management to increase soil and crop health, however, the environmental externalities such as greenhouse gas emissions (GHG) from cropping systems that utilize organic fertilizers, such as compost, is uncertain. Vermicompost, which is compost created through the digestion of organic matter by earthworms, and its related ‘extract’ are commonly thought to provide more benefits for soil and crop health compared to standard compost. However, organic fertilizers such as vermicompost, vermicompost extract, and compost have not been compared in their effects on soil health in Mediterranean vineyards. In this two-year study, I assessed …

Objectively Measured Physical Activity Is Lower In Individuals With Normal Weight Obesity In The United States, Nadeeja N. Wijayatunga, Heontae Kim, Harry M. Hays, Minsoo Kang

Faculty and Student Publications

The role of physical activity in normal weight obesity (NWO), which is associated with increased cardiometabolic risk, is not clear. This study aimed to determine body composition phenotype-specific differences in objectively measured physical activity and sedentary time in adults in the United States. A total of 2055 adults with a body mass index (BMI) ≥ 18.5 m2 were studied using 2003–2006 National Health and Nutrition Examination Surveys. Physical activity and percent body fat (BF%) were measured using accelerometer and dual-energy X-ray absorptiometry, respectively. A BF% > 23.1% and >33.3% for men and women, respectively, was considered excess. A BMI of 18.5–24.9 …

Sex Impact On Knee And Ankle Muscle Extensor Forces During Loaded Running, Kade D. Wagers, Nicholas J. Lobb, Auralea C. Fain, Kayla D. Seymore, Tyler N. Brown

Kinesiology Faculty Publications and Presentations

Background: This study determined whether the knee and ankle muscle extensor forces increase when running with a body-borne load and whether these forces differ between the sexes. Methods: Thirty-six (twenty male and sixteen female) adults had the knee and ankle extensor force quantified when running 4.0 m/s with four body-borne loads (20, 25, 30, and 35 kg). Peak normalized (BW) and unnormalized (N) extensor muscle force, relative effort, and joint angle and angular velocity at peak muscle force for both the ankle and the knee were submitted to a mixed model ANOVA. Results: Significant load by sex interactions for knee …

Promoting Validation And Cross-Phylogenetic Integration In Model Organism Research., Keith C Cheng, Rebecca D Burdine, Mary E Dickinson, Stephen C Ekker, Alex Y Lin, K C Kent Lloyd, Cathleen Lutz, Calum A Macrae, John H Morrison, David H O'Connor, John H Postlethwait, Crystal D Rogers, Susan Sanchez, Julie H Simpson, William S Talbot, Douglas C Wallace, Jill M Weimer, Hugo J Bellen

Faculty Research 2022

Model organism (MO) research provides a basic understanding of biology and disease due to the evolutionary conservation of the molecular and cellular language of life. MOs have been used to identify and understand the function of orthologous genes, proteins, cells and tissues involved in biological processes, to develop and evaluate techniques and methods, and to perform whole-organism-based chemical screens to test drug efficacy and toxicity. However, a growing richness of datasets and the rising power of computation raise an important question: How do we maximize the value of MOs? In-depth discussions in over 50 virtual presentations organized by the National …

Genetically Diverse Mouse Platform To Xenograft Cancer Cells, Jennifer K Sargent, Mark A. Warner, Benjamin E. Low, William H. Schott, Todd Hoffert, David Coleman, Xing Yi Woo, Todd B Sheridan, Sonia Erattupuzha, Philipp P Henrich, Vivek M. Philip, Jeffrey Chuang, Michael V. Wiles, Muneer G. Hasham

Faculty Research 2022

The lack of genetically diverse preclinical animal models in basic biology and efficacy testing has been cited as a potential cause of failure in clinical trials. We developed and characterized five diverse RAG1 null mouse strains as models that allow xenografts to grow. In these strains, we characterized the growth of breast cancer, leukemia and glioma cell lines. We found a wide range of growth characteristics that were far more dependent on strain than tumor type. For the breast cancer cell line, we characterized the spectrum of xenograft/tumor growth at structural, histological, cellular and molecular levels across each strain, and …

Enhanced Development Of Functional Human Nk Cells In Nod-Scid-Il2rg Mice Expressing Human Il15, Ken-Edwin Aryee, Lisa M. Burzenski, Li-Chin Yao, James G. Keck, Dale L. Greiner, Leonard D. Shultz, Michael A. Brehm

Faculty Research 2022

Human innate immunity plays a critical role in tumor surveillance and in immunoregulation within the tumor microenvironment. Natural killer (NK) cells are innate lymphoid cells that have opposing roles in the tumor microenvironment, including NK cell subsets that mediate tumor cell cytotoxicity and subsets with regulatory function that contribute to the tumor immune suppressive environment. The balance between effector and regulatory NK cell subsets has been studied extensively in murine models of cancer, but there is a paucity of models to study human NK cell function in tumorigenesis. Humanized mice are a powerful alternative to syngeneic mouse tumor models for …

Pts Is Activated By Atf4 And Promotes Lung Adenocarcinoma Development Via The Wnt Pathway, Wei Ma, Chao Wang, Ruzhen Li, Zhaohui Han, Yuanzhu Jiang, Xiangwei Zhang, Duilio Divisi, Enrico Capobianco, Lin Zhang, Wei Dong

Faculty Research 2022

BACKGROUND: The effects and mechanism of 6-pyruvoyl-tetrahydropterin synthase (

METHODS:

RESULTS:

CONCLUSIONS:

Planning System For The Optimization Of Electric Field Delivery Using Implanted Electrodes For Brain Tumor Control, Erin Iredale, Brynn Voigt, Adam Rankin, Kyungho W Kim, Jeff Z Chen, Susanne Schmid, Matthew O Hebb, Terry M Peters, Eugene Wong

Anatomy and Cell Biology Publications

BACKGROUND: The use of non-ionizing electric fields from low-intensity voltage sources (< 10 V) to control malignant tumor growth is showing increasing potential as a cancer treatment modality. A method of applying these low-intensity electric fields using multiple implanted electrodes within or adjacent to tumor volumes has been termed as intratumoral modulation therapy (IMT).

PURPOSE: This study explores advancements in the previously established IMT optimization algorithm, and the development of a custom treatment planning system for patient-specific IMT. The practicality of the treatment planning system is demonstrated by implementing the full optimization pipeline on a brain phantom with robotic electrode implantation, postoperative imaging, and treatment stimulation.

METHODS: The integrated planning pipeline in 3D Slicer begins with importing and segmenting patient magnetic resonance images (MRI) or computed tomography (CT) images. The segmentation process is manual, followed by a semi-automatic smoothing step that allows …

A Review Of Current Practices In Periprosthetic Joint Infection Debridement And Revision Arthroplasty, Humza M. Mian, Joseph G. Lyons, Joshua D. Perrin, Andrew W. Froehle, Anil Krishnamurthy

School of Health and Exercise Sciences Faculty Publications

Background: Periprosthetic joint infection remains a significant challenge for arthroplasty surgeons globally. Over the last few decades, there has been much advancement in terms of treatment and diagnosis, however, the fight rages on. As management of periprosthetic joint infections continues to evolve, it is critical to reflect back on current debridement practices to establish common ground as well as identify areas for future research and improvement. BODY: In order to understand the debridement techniques of periprosthetic joint infections, one must also understand how to diagnose a periprosthetic joint infection. Multiple definitions have been elucidated over the years with no single …

The Casnr L.I.N.K.S. Newsletter: Learning Innovation Network For K-12 Schools, Edition 8, September 2022, Bailey Feit, Tammy Mittelstet

CASNR L.I.N.K.S. Newsletter

The purpose of this L.I.N.K.S. newsletter is to provide a monthly update of opportunities and resources for K-12 schools to connect with the University of Nebraska's CASNR Food, Energy, Water, and Societal Systems (FEWS²) programs and partners.

Included in this edition:

Curriculum and classroom resources for all levels

Eye on curriculum and resources

K-12 Partners website is live

Ineda Nebraska Ag Expo career exploration event

Teacher opportunities

Link to list of new partnerships

Ask an expert

Eye on CASNR research: Eric Knoll

Student experiences

Sens your students to campus: Husker weekday visit

Success stories: University TAPS Program coming soon

Head Removal Enhances Planarian Electrotaxis, Ziad Sabry , '21, Rui Wang, A. Jahromi, Christina Rabeler, W. B. Kristan Iii, Eva-Maria S. Collins

Biology Faculty Works

Certain animal species utilize electric fields for communication, hunting and spatial orientation. Freshwater planarians move toward the cathode in a static electric field (cathodic electrotaxis). This planarian behavior was first described by Raymond Pearl more than a century ago. However, planarian electrotaxis has received little attention since, and the underlying mechanisms and evolutionary significance remain unknown. To close this knowledge gap, we developed an apparatus and scoring metrics for automated quantitative and mechanistic studies of planarian behavior upon exposure to a static electric field. Using this automated setup, we characterized electrotaxis in the planarian Dugesia japonica and found that this …

Pestfacts Wa Issue 16 - September 2022, Cindy L. Webster, Dusty Severtson, Alan Lord, Jean Galloway, Ciara Beard, Geoff J. Thomas, Andrea Hills

PestFacts WA

PestFacts WA Issue 16 - September 2022:

• Spring control of redlegged earth mites
• Native budworm update
• Botrytis grey mould
• Anthracnose and Phomopsis in lupins
• Leaf rust in oats and wheat

Skeleton Weed In Western Australia / Management Guide, Department Of Primary Industries And Regional Development, Western Australia

Bulletins 4000 -

Skeleton weed is a declared plant which can reduce crop yields by competing for moisture and nutrients (mainly nitrogen).

This Management Guide has been designed to assist landholders and increase their capacity to manage and eradicate skeleton weed infestations on their properties and to prevent further spread within the State.

Without the coordinated program aimed at controlling spread, skeleton weed would now be much more abundant and widely established throughout the cereal growing areas.

Pestfacts Wa Issue 17 - September 2022, Cindy L. Webster, Svetlana Micic, Alan Lord, Ciara Beard, Andrea Hills, Daniel Huberli

PestFacts WA

PestFacts WA Issue 17 - September 2022 contents:

• Armyworm update
• Clean augers, field bins and silos to prevent insects contaminating grain
• Flag smut in wheat
• PestFacts WA Reporter app now available for android devices