Life Sciences Commons^™

Single-Cell Sequencing Reveals Trajectory Of Tumor-Infiltrating Lymphocyte States In Pancreatic Cancer, Aislyn Schalck, Donastas Sakellariou-Thompson, Marie-Andrée Forget, Emi Sei, Tara G Hughes, Shanshan Bai, Tapsi Kumar, Mark W Hurd, Matthew H G Katz, Ching-Wei D Tzeng, Shubham Pant, Milind Javle, David R Fogelman, Anirban Maitra, Cara L Haymaker, Michael P Kim, Nicholas E Navin, Chantale Bernatchez

Faculty, Staff and Student Publications

Pancreatic ductal adenocarcinoma (PDAC) has few effective treatments. Immunotherapy, an attractive alternative strategy, remains challenging with the lack of knowledge on the tumor-infiltrating lymphocyte (TIL) landscape in PDAC. To generate a reference of T-cell subpopulations, we profiled 80,000 T cells from 57 PDAC samples, 22 uninvolved/normal samples, and cultured TIL using single-cell transcriptomic and T-cell receptor analysis. These data revealed 20 cell states and heterogeneous distributions of TIL populations. The CD8+ TIL contained a putative transitional GZMK+ population based on T-cell receptor clonotype sharing, and cell-state trajectory analysis showed similarity to a GZMB+PRF1+ cytotoxic and a CXCL13+ dysfunctional population. Statistical …

Single-Cell Transcriptomic Profiling Reveals The Tumor Heterogeneity Of Small-Cell Lung Cancer, Yanhua Tian, Qingqing Li, Zhenlin Yang, Shu Zhang, Jiachen Xu, Hua Bai, Bo Zheng, Wen Li, Yueli Cui, Xin Wang, Rui Wan, Kailun Fei, Jia Zhong, Shugeng Gao, Jie He, Carl M Gay, Jianjun Zhang, Jie Wang, Fuchou Tang

Faculty, Staff and Student Publications

Small-cell lung cancer (SCLC) is the most aggressive and lethal subtype of lung cancer, for which, better understandings of its biology are urgently needed. Single-cell sequencing technologies provide an opportunity to profile individual cells within the tumor microenvironment (TME) and investigate their roles in tumorigenic processes. Here, we performed high-precision single-cell transcriptomic analysis of ~5000 individual cells from primary tumors (PTs) and matched normal adjacent tissues (NATs) from 11 SCLC patients, including one patient with both PT and relapsed tumor (RT). The comparison revealed an immunosuppressive landscape of human SCLC. Malignant cells in SCLC tumors exhibited diverse states mainly related …

Differences In Neurotoxic Outcomes Of Organophosphorus Pesticides Revealed Via Multi-Dimensional Screening In Adult And Regenerating Planarians, Danielle Ireland, S. Zhang, Veronica Bochenek , ' 22, J.-H. Hsieh, Christina Rabeler, Zane Meyer , '21, Eva-Maria S. Collins

Biology Faculty Works

Organophosphorus pesticides (OPs) are a chemically diverse class of commonly used insecticides. Epidemiological studies suggest that low dose chronic prenatal and infant exposures can lead to life-long neurological damage and behavioral disorders. While inhibition of acetylcholinesterase (AChE) is the shared mechanism of acute OP neurotoxicity, OP-induced developmental neurotoxicity (DNT) can occur independently and/or in the absence of significant AChE inhibition, implying that OPs affect alternative targets. Moreover, different OPs can cause different adverse outcomes, suggesting that different OPs act through different mechanisms. These findings emphasize the importance of comparative studies of OP toxicity. Freshwater planarians are an invertebrate system that …

Morphometric And Molecular Characterization Of An Unpigmented Haemosporidian Parasite In The Neotropical Turnip-Tailed Gecko (Thecadactylus Rapicauda), Nubia E. Matta, Leydy P. González, Mario Vargas-Ramírez, Gediminas Valkiūnas, Ananias A. Escalante, M. Andreína Pacheco

Harold W. Manter Laboratory of Parasitology: Library Materials

Morphological traits from blood stages have been the gold standard for determining haemosporidian parasite species. However, the status of some taxa and the value of such traits in parasites from reptiles remain contentious. The scarce sampling of these species worsens the situation, and several taxa lack molecular data. A survey was performed in the Magdalena Department in Colombia, where 16 species of reptiles were captured. A peculiar haemosporidian parasite was found in the Turnip-tailed gecko Thecadactylus rapicauda. This haemosporidian does not show malarial pigment in blood stages under light microscopy; thus, it fits the Garnia genus’s characters belonging to …

Distinct Immune Signature Predicts Progression Of Vestibular Schwannoma And Unveils A Possible Viral Etiology, Moran Amit, Tongxin Xie, Frederico O Gleber-Netto, Patrick J Hunt, Gautam U Mehta, Deborah A Silverman, Yi Ye, Jared K Burks, Gregory N Fuller, Paul W Gidley, Marc-Elie Nader, Shaan M Raza, Franco Demonte

Faculty, Staff and Student Publications

BACKGROUND: The management of sub-totally resected sporadic vestibular schwannoma (VS) may include observation, re-resection or irradiation. Identifying the optimal choice can be difficult due to the disease's variable progression rate. We aimed to define an immune signature and associated transcriptomic fingerprint characteristic of rapidly-progressing VS to elucidate the underpinnings of rapidly progressing VS and identify a prognostic model for determining rate of progression.

METHODS: We used multiplex immunofluorescence to characterize the immune microenvironment in 17 patients with sporadic VS treated with subtotal surgical resection alone. Transcriptomic analysis revealed differentially-expressed genes and dysregulated pathways when comparing rapidly-progressing VS to slowly or …

Biophysics Of Cancer, Alemayehu A Gorfe

Faculty, Staff and Student Publications

No abstract provided.

Perennial Pepperweek Id Card_2022, Malin Clyde, Charlotte Thompson

UNH Cooperative Extension

No abstract provided.

Exploring The Barriers And Facilitators To Making Healthy Physical Activity Lifestyle Choices Among Uk Bame Adults During Covid-19 Pandemic: A Study Protocol, Johnson Mbabazi, Fiona Macgregor, Mona Salman, Jeff Breckon, Edward Kunonga, Barry Tolchard, Lawrence Achilles Nnyanzi

International Journal of Physical Activity and Health

Past research has identified that individuals from BAME communities face health inequalities and report poorer outcomes from numerous health interventions. This study will explore some of the reasons with a focus on the perceptions towards physical activity in the lifestyle prevention of diseases. It will also seek to elicit a range of facilitators and barriers towards improving physical activity lifestyle choices amongst UK BAME adults, including but not limited to those in the individual, structural, environmental and social domains. Furthermore, it will consider the role of ethnicity and culture in the forming of physical activity lifestyle choices. This study was …

Key Issues Generated From The Xi International Rangeland Congress 2021: Summary And Way Forward, D. L. Coppock, A. J. Ash, B. Irving

IGC Proceedings (1977-2023)

The important issues, knowledge gaps, and evolving research approaches for the global rangelands are summarised in this review of submissions to the Joint XXIV International Grasslands and XI International Rangelands Congress (IGC/IRC). In the big picture, it is concluded that stand-alone studies of livestock production are becoming rare compared to that of the past International Rangelands Congresses (IRC). Rather, added effort is now being directed at understanding the fuller context of social-ecological systems (SESs) on rangelands in a quest to improve the prospects for sustainable resource management as well as the enhancement of human welfare. Although climate change is upon …

Joint International Grassland And International Rangeland Congress Kenya 2021: Grasslands Summary, James L. Klotz, J. H. Bouton

IGC Proceedings (1977-2023)

This summary used as its main sources the plenary and keynote papers and talks in Sub-theme 2: Forage Production and Utilization and Sub-theme 3: Livestock Production Systems as well as a sampling of oral talks presented at the XXIV International Grassland Congress (IGC). This IGC was held virtually and jointly with the International Rangeland Congress and hosted in Nairobi, Kenya during 23-29 October 2021. The XXIV IGC was also the first to be held on the African continent in Congress' 94-year history. The summary is further focused on that information presented under the concept "cultivated grasslands" (e.g., a small number …

Identification And Antibiotic-Susceptibility Profiling Of Non-Hospital Wound Infecting Staphylococcus Aureus Isolated From Ghail-Bawazeer Patients, Yemen, Maged Salem Bin-Masalam, A. Ba-Swaqi, A. Hwydi, A. Mhedy, E. Alamudi, H. Bin-Nashe, M. Abozid, S. Aljomhy, S. Badaood

Hadhramout University Journal of Natural & Applied Sciences

The continuous increase in the resistance of pathogenic Staphylococcus aureus isolated from non-hospital infected wounds to the novel chemical classes of antibiotics is of great public health importance. This problem prompted the researchers to study the antibiotic susceptibility of such strains. Twenty wound pus samples were collected and selected randomly from 20 non-hospital wound infected patients of different ages who attended different medical laboratories in Ghail-Bawazeer, Yemen, from October to December 2020, and 24 bacterial isolates were analyzed using morphological, cultural, and biochemical characterization for the isolation and identification of S. aureus. Based on the morphological and cultural characteristics, …

Effects Of Nitrogen Fertilizer On The Sugar And Ethanol Production Of C. Sativa, Aidan Power, Christian Mugisha, Boanerges Bamaca, Asmita Mahara, Mark Wilkins Ph.D

Annual Student Research Poster Session

With the decrease of fossil fuel-supply and increase of greenhouse gasses, biofuels are needed now more than ever. This includes bioethanol, an alternative fuel that can be made from waste materials, including C. Sativa (commonly known as hemp). The common belief in biological engineering is that the sugars from hemp can attract fermenting organisms to produce the needed ethanol. The question remains, however, of how external factors play into the ethanol production from hemp, such as fertilizer. I tested samples from three plots with different amounts of nitrogen fertilizer to see if the amount of sugar and ethanol varies or …

Identifying Androgens As A Protective Agent In The Allergic Immune Response To Peanut, Mckenna Vininski

Biology Theses, Dissertations, and Student Creative Activity

Peanut (PN) allergy is a major public health concern. Prevalence of PN allergy has been on the rise for decades due to poor prevention information and a lack of knowledge about the allergic mechanism. Recent research has brought clarity about how individuals become sensitized with routes known through the skin, as well as the airway. Factors influence the allergic immune response, however, based on a known sex difference in allergic asthma and food allergy demographics showing a sex difference, this thesis work hypothesized that a sex difference would exist in PN allergy. This study utilized a 4-week inhalation mouse model …

Racial And Ethnic Differences In The Association Of Low-Carbohydrate Diet With Mortality In The Multi-Ethnic Study Of Atherosclerosis, Seung-Won Oh, Alexis C Wood, Seung-Sik Hwang, Matthew Allison

Children’s Nutrition Research Center Staff Publications

Importance: Evidence linking low-carbohydrate diets (LCDs) to mortality is limited among multiethnic populations.

Objective: To evaluate the association between LCDs and mortality by race and ethnicity.

Design, setting, and participants: The Multi-Ethnic Study of Atherosclerosis is a large, population-based prospective cohort study of adults aged 45 to 84 years recruited from 6 US communities. A total of 6109 participants without baseline cardiovascular disease were included in this analysis. Baseline data were collected from July 2000 to August 2002, with follow-up completed by December 2017. The data were analyzed between May 2021 and April 2022.

Exposures: Food frequency questionnaires were used …

X-Linked Ubiquitin-Specific Peptidase 11 Increases Tauopathy Vulnerability In Women, Yan Yan

USF Tampa Graduate Theses and Dissertations

Women experience significantly higher tau burden and increased risk for Alzheimer’s disease (AD) than men, yet the underlying mechanism for this vulnerability has not been explained. Here, we demonstrate through in vitro and in vivo models, as well as human AD brain tissue, that X-linked ubiquitin specific peptidase 11 (USP11) augments pathological tau aggregation via tau deubiquitination initiated at lysine-281. Removal of ubiquitin provides access for enzymatic tau acetylation at lysines 281 and 274. USP11 escapes complete X-inactivation, and female mice and people both exhibit higher USP11 levels than males. Genetic elimination of usp11 in a tauopathy mouse model preferentially …

Polyamine Enzymes As Potential Targets For Cancer Therapeutics To Mitigate Diabetic Triple Negative Breast Cancer Advancement, Hadassha Tofilau

Biology Theses, Dissertations, and Student Creative Activity

Breast cancer (BC) is the second leading cause of death in women. Co-existence of diabetes and BC can be lethal, leading to higher mortality than BC alone. TNBC is highly refractive, heterogenous, and resistant to most chemotherapies. Polyamines play a role in cell growth and are elevated in cancer, though their role in diabetic TNBC has not been explored. We hypothesized that high glucose/diabetic conditions increase TNBC cell proliferation through modification of enzymes in the polyamine pathway and that miR-133a would decrease TNBC cell proliferation. For this study, MDA-MB-231 and MCF-10A cell lines were both treated with normal (5mM) and …

Interaction Between Age And Fatigue On Antagonist Muscle Coactivation During An Acute Post-Fatigue Recovery Phase, Sara A. Harper, Brennan J. Thompson

Kinesiology and Health Science Faculty Publications

This study investigated the age-related changes in antagonist muscle coactivation of the biceps femoris (BF) during an acute recovery period following a leg extensor fatiguing protocol. Twenty-three young (mean ± SD: age = 25.1 ± 3.0 years) and twenty-three old men (age = 71.5 ± 3.9 years) participated. Surface electromyography (sEMG) was recorded from the BF muscles for antagonist muscle coactivation. Testing invovled participants performing leg extension isometric maximal voluntary contractions (MVCs) and isokinetic MVCs at 240°•s^-1 at baseline (Pre) and again after the fatigue protocol at 0 (Post0), 7 (Post7), 15 (Post15), and 30 (Post30) minutes post fatigue. …

Values: Helping Agricultural Producers Find Motivation By Focusing On What Matters, Jacob D. Gossner, Elizabeth Fauth, Tasha Howard

All Current Publications

This fact sheet addresses the importance of tuning into values as a way of maintaining motivation and promoting mental health for agricultural producers.

Classification Of High-Grade Serous Ovarian Cancer Using Tumor Morphologic Characteristics, Katelyn F Handley, Travis T Sims, Nicholas W Bateman, Deanna Glassman, Katherine I Foster, Jun Yao, Bryan M Fellman, Jinsong Liu, Zhen Lu, Kelly A Conrads, Brian L Hood, Waleed Barakat, Li Zhao, Jianhua Zhang, Shannon N Westin, Joseph Celestino, Kelly M Rangel, Sunil Badal, Igor Pereira, Prahlad T Ram, George L Maxwell, Livia S Eberlin, P Andrew Futreal, Robert C Bast, Nicole D Fleming, Thomas P Conrads, Anil K Sood

Faculty, Staff and Student Publications

IMPORTANCE: Despite similar histologic appearance among high-grade serous ovarian cancers (HGSOCs), clinical observations suggest vast differences in gross appearance. There is currently no systematic framework by which to classify HGSOCs according to their gross morphologic characteristics.

OBJECTIVE: To develop and characterize a gross morphologic classification system for HGSOC.

DESIGN, SETTING, AND PARTICIPANTS: This cohort study included patients with suspected advanced-stage ovarian cancer who presented between April 1, 2013, and August 5, 2016, to the University of Texas MD Anderson Cancer Center, a large referral center. Patients underwent laparoscopic assessment of disease burden before treatment and received a histopathologic diagnosis of …

Family Planning, Fertility, And Career Decisions Among Female Oncologists, Anna Lee, Aleksandra Kuczmarska-Haas, Shraddha M Dalwadi, Erin F Gillespie, Michelle S Ludwig, Fumiko Chino

Faculty, Staff and Student Publications

Importance: Female oncologists often spend their childbearing years in training and establishing careers, with many later experiencing fertility issues when starting a family. Physician fertility and family planning are rarely discussed during training. Attitudes among female oncologists regarding family planning are unknown.

Objectives: To understand barriers to family planning as well as the association of fertility treatment with career decisions and to assess experiences of pregnancy-based discrimination among female oncologists.

Design, setting, and participants: In this survey study, a novel 39-item questionnaire was distributed to US female oncologists from May 7 to June 30, 2020, via email and social media …

Brain Metastases: A Society For Neuro-Oncology (Sno) Consensus Review On Current Management And Future Directions, Ayal A Aizer, Nayan Lamba, Manmeet S Ahluwalia, Kenneth Aldape, Adrienne Boire, Paul D Brown, Veronica L Chiang, Michael A Davies, Leland S Hu, Raymond Y Huang, Timothy Kaufmann, Priya Kumthekar, Keng Lam, Eudocia Q Lee, Nancy U Lin, Minesh Mehta, Michael Parsons, David A Reardon, Jason Sheehan, Riccardo Soffietti, Hussein Tawbi, Michael Weller, Patrick Y Wen

Faculty, Staff and Student Publications

Brain metastases occur commonly in patients with advanced solid malignancies. Yet, less is known about brain metastases than cancer-related entities of similar incidence. Advances in oncologic care have heightened the importance of intracranial management. Here, in this consensus review supported by the Society for Neuro-Oncology (SNO), we review the landscape of brain metastases with particular attention to management approaches and ongoing efforts with potential to shape future paradigms of care. Each coauthor carried an area of expertise within the field of brain metastases and initially composed, edited, or reviewed their specific subsection of interest. After each subsection was accordingly written, …

Association Of Prediagnosis Obesity And Postdiagnosis Aspirin With Survival From Stage Iv Colorectal Cancer, Jennifer S Davis, Janelle C Chavez, Melissa Kok, Yazmin San Miguel, Hwa Young Lee, Michael J Overman, Bryan Kee, David Fogelman, Shailesh M Advani, Benny Johnson, Christine Parseghian, John Paul Shen, Arvind Dasari, Kenna R Shaw, Eduardo Vilar, Kanwal P Raghav, Imad Shureiqi, Robert A Wolff, Funda Meric-Bernstam, Dipen Maru, David G Menter, Scott Kopetz, Shine Chang

Faculty, Staff and Student Publications

IMPORTANCE: The potential relationship between obesity and colorectal cancer (CRC) outcome is poorly understood in patients with late-stage disease. Increased body mass index may negate aspirin use for cancer prevention, but its role as a factor on the effectiveness of postdiagnosis aspirin use is unclear.

OBJECTIVE: To evaluate how prediagnosis obesity and postdiagnosis aspirin use may be associated with overall survival in patients with late-stage colorectal cancer.

DESIGN, SETTING, AND PARTICIPANTS: This cross-sectional study used self-reported data from patients with metastatic or treatment-refractory disease who consented to a clinical protocol at MD Anderson Cancer Center, a large US cancer treatment …

Egfr Suppresses P53 Function By Promoting P53 Binding To Dna-Pkcs: A Noncanonical Regulatory Axis Between Egfr And Wild-Type P53 In Glioblastoma, Jie Ding, Xiaolong Li, Sabbir Khan, Chen Zhang, Feng Gao, Amanda R Wasylishen, Guillermina Lozano, Dimpy Koul, W K Alfred Yung

Faculty, Staff and Student Publications

Background: Epidermal growth factor receptor (EGFR) amplification and TP53 mutation are the two most common genetic alterations in glioblastoma multiforme (GBM). A comprehensive analysis of the TCGA GBM database revealed a subgroup with near mutual exclusivity of EGFR amplification and TP53 mutations indicative of a role of EGFR in regulating wild-type-p53 (wt-p53) function. The relationship between EGFR amplification and wt-p53 function remains undefined and this study describes the biological significance of this interaction in GBM.

Methods: Mass spectrometry was used to identify EGFR-dependent p53-interacting proteins. The p53 and DNA-dependent protein kinase catalytic subunit (DNA-PKcs) interaction was detected by co-immunoprecipitation. We …

Impact Of Treatment Modality On Pelvic Floor Dysfunction Among Uterine Cancer Survivors, David S Lakomy, Alison K Yoder, Juliana Wu, Mike Hernandez, Martins Ayoola-Adeola, Ann Klopp, Susan K Peterson, Lilie L Lin

Faculty, Staff and Student Publications

Objective: Pelvic floor dysfunction is a common adverse effect of uterine cancer treatment. In this study we compared patient-reported outcomes regarding pelvic floor dysfunction among uterine cancer survivors after hysterectomy and bilateral salpingo-oophorectomy, surgery and brachytherapy, or surgery and external beam radiotherapy with or without brachytherapy versus women who had a hysterectomy for benign indications.

Methods: We used the validated 20-item Pelvic Floor Distress Inventory to assess lower urinary distress, colorectal distress, and pelvic organ prolapse dysfunction in each treatment group. Pelvic floor dysfunction-related quality of life in these domains was compared across treatment modalities using the Pelvic Floor Impact …

A New Species Of Scorpiops Peters, 1861 From Yunnan Province, China, With A Preliminary Review Of Its Congeners In Yunnan (Scorpiones: Scorpiopidae), Victoria Tang

Euscorpius

A new species of Scorpiops Peters, 1861, Scorpiops tongtongi sp. n., is described from Yingjiang, Yunnan Province, China, based on a single adult female. The morphology of the new species is fairly distinguishable from other congeners in Yunnan even with unaided eye; it is characterized by the following combination of characters in female: a pair of moderately robust pedipalp chelae without proximal lobes, small median ocular tubercle, short superciliary carinae, less granulated tergites, proportionally elongate metasoma and relatively short and bulbous vesicle. The coloration of the new species also differs from other congeners in Yunnan by being brownish in …

Physicochemical Analysis Of Cold Brew And Hot Brew Peaberry Coffee, Evan T. Schwarzmann, Marlena P. Washington, Niny Z. Rao

College of Life Sciences Faculty Papers

Peaberry coffee is the result of a natural mutation of coffee beans, and they make up only about 5–7% of coffee crops. A typical coffee cherry contains two seeds that are developed against each other, resulting in the distinctive half-rounded shape of coffee beans. However, failing to fertilize both ovules of one of the seeds or failure in endosperm development can cause only one of the seeds to develop, resulting in smaller, denser beans with a more domed shape. Peaberry coffees are said to be sweeter, lighter, and more flavorful since the peaberry beans receive all nutrients from the coffee …

Speciation Jigsaw Activity, Erika Crispo

Open Educational Resources

What is a species? Why are there so many species on the planet? The question of why we have different species of organisms was the key thesis of Charles Darwin’s most famous book, On the Origin of Species.While several definitions of what constitutes a species exists, the most common definition lies within thebiological species concept. This concept states that different species exist when two individuals of different sexes are unable to reproduce with one another to yield viable offspring in the wild. A major limitation of this definition is that it can only be applied to sexually reproducing organisms that …

Clinical Practice Guidelines: The Road Map To Better Care, Mary I. Fisher

Physical Therapy Faculty Publications

Systematic reviews and meta-analyses are important tools in translating research evidence into clinical practice as they critically evaluate and summarize the body of evidence on clinical topics. Clinicians can use these studies to quickly survey the current literature base to increase understanding of a clinical diagnosis they make, with the intent of using this information to improve their clinical practice. A well-written systematic review or meta-analysis, with critical evaluation of study quality, is then that Cliffs Note version of what to know and what to do. Therefore, with the proliferation of systematic reviews and meta-analyses, we should see a corresponding …

Building Soil By Building Community: How Can An Interdisciplinary Approach Better Support Community Needs And Urban Resilience, Erica L. Wohldmann, Yujuan Chen, Kirsten Schwarz, Susan D. Day, Richard V. Pouyat, Michelle Barton, Manny Gonez

Agricultural and Environmental Sciences Faculty Research

Given the interrelated problems of climate change, energy and resource scarcity, and the challenge of supporting critical natural systems in cities, urban dwellers may be exceptionally vulnerable to the impacts of climate change. While a number of programs and policies have been developed and implemented to help reduce the environmental and social impacts of climate change on communities, we argue that effective and sustainable programs must not only consider how the changing environment impacts communities, but also how communities interact with and impact the environment. Specifically, drawing on a case study of the needs assessment of the Healthy Soils for …

Comparative Transcriptomics Reveals Small Rna Composition And Differential Microrna Responses Underlying Interferon-Mediated Antiviral Regulation In Porcine Alveolar Macrophages, Jiuyi Li, Eric R. Sang, Oluwaseun Adeyemi, Laura C. Miller, Yongming Sang

Agricultural and Environmental Sciences Faculty Research

Previous studies have shown that interferon-mediated antiviral activity is subtype-dependent. Using a whole transcriptome procedure, we aimed to characterize the small RNA transcriptome (sRNA-Seq) and specifically the differential microRNA (miRNA) responses in porcine alveolar macrophages (PAMs) upon antiviral activation during viral infection and interferon (IFN) stimulation. Data showed that near 90% of the qualified reads of sRNA were miRNAs, and about 10% of the other sRNAs included rRNA, snoRNA, snRNA, and tRNA in order of enrichment. As the majority of sRNA (>98%) were commonly detected in all PAM samples under different treatments, about 2% sRNA were differentially expressed between …