Life Sciences Commons^™

Defective Thyroglobulin: Cell Biology Of Disease, Xiaohan Zhang, Crystal Young, Yoshiaki Morishita, Kookjoo Kim, Omer O. Kabil, Oliver B. Clarke, Bruno Di Jeso, Peter Arvan

Faculty Scholarship

The primary functional units of the thyroid gland are follicles of various sizes comprised of a monolayer of epithelial cells (thyrocytes) surrounding an apical extracellular cavity known as the follicle lumen. In the normal thyroid gland, the follicle lumen is filled with secreted protein (referred to as colloid), comprised nearly exclusively of thyroglobulin with a half-life ranging from days to weeks. At the cellular boundary of the follicle lumen, secreted thyroglobulin becomes iodinated, resulting from the coordinated activities of enzymes localized to the thyrocyte apical plasma membrane. Thyroglobulin appearance in evolution is essentially synchronous with the appearance of the follicular …

Evaluating Acid-Aluminum Stress In Streams Of The Northeastern U.S. At Watershed, Fish Community And Physiological Scales, Benjamin J. Zdasiuk, Celia Y. Chen, Stephen D. Mccormick, Keith H. Nislow, Joel Singley, John T. Kelly

Arts & Sciences Faculty Publications

In spite of overall improvements in air and water quality, biological stress from low pH and high concentrations of inorganic aluminum continue to impact fish and fish habitat in northeastern North America, with independent and interactive effects on individuals, populations and communities. Integrative indicators can therefore be useful in monitoring both impact and recovery across multiple scales. Using coupled water chemistry (pH, conductivity, and base cation and inorganic aluminum concentration), geographic (site elevation and watershed area) and biological (fish diversity, fish abundance, gill aluminum concentration and gill physiology) data, we developed an integrated indicator of acid-aluminum stress across the White …

College Of Natural Sciences Newsletter, September & October 2022, College Of Natural Sciences

College of Natural Sciences Newsletters and Reports

Volume 3, Issue 6

Page 1 Dean's Message
Page 2 Awards & Recognition
Page 3 Midwest Regional ACS Meeting
Page 4 North Central ASM Meeting
Page 5 Geography Department Travel
Page 6-7 Media Coverage of CNS
Page 7 REMAST Program at SDState receives national spotlight
Page 8 Celebrating the lives of those who touched the College
Page 9 Innovative Learning Spaces
Page 10 Open PRAIRIE Data
Page 11 2022 CNS Scholarship Brunch
Page 12-14 Fall 2022 Outreach Events

Association Of A Green Tea Extract With Serum Immunoglobulin G Status And Neonatal Vitality In Newborn Dairy Calves, M. E. Reis, Melissa C. Cantor, C. M. M. Bittar, Joao H. C. Costa

Animal and Food Sciences Faculty Publications

Poor vigor at birth has been associated with reduced IgG absorption from colostrum and a reduced vitality in neonatal dairy calves. Some natural compounds, such as green tea extract, may improve vitality in compromised calves. The objective of this randomized controlled trial was to evaluate the potential of supplementing a green tea extract (15 mL) to calves to improve vigor and activity behavior for the first 72 h postnatal. Also, this study aimed to investigate the influence of green tea extract supplementation on calf serum IgG concentration and the apparent efficiency of absorption (AEA) of colostral IgG. Holstein calves (n …

Louis Pasteur Signature Saying: Science Brings Men Closer To God Bicentenary Year: 200 Years After His Birth, Alan L. Gillen, Justin A. Ambrose

Faculty Publications and Presentations

This year (2022) marks the bicentenary birth (December 27, 1822) of Louis Pasteur. He has a global reputation. His contributions can be seen in our homes, in the "pasteurized" products derived from one of his discoveries and in disease prevention. It was in his discovery of gently heating beverages and food for what we today call pasteurization that he first said: “Man's first look at the universe discovers only variety, diversity, multiplicity of phenomena. Let this gaze be enlightened by science — by science which brings man closer to God — and simplicity and unity shine everywhere.” Louis Pasteur (Vallery-Radot, …

Impacts Of Algal Morphology And Water Flow On Macroalgal Microplastic Capture, Cheyenne M. Adams

Honors College Theses

Microplastic pollution is a major area of concern in marine environments, especially as microplastics enter the food web. This study used pipe cleaners and two lichen species as algal mimics, and Chaetomorpha sp. and Chondrus crispus as model algal species to test the effects of morphology and biomass on microbead and foam capture. This study also utilized two different water flow methods: vortices and waves. Results suggest that water flow, as well as biomass and morphology, play a role in microplastic capture in macroalgae. For all mimics and algal species, except Cladonia lichens, turfs with increased biomass and length showed …

Nebline, November/December 2022

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Holiday Wellness Tips by Alyssa Havlovic

Holiday Food Safety Quiz by Alyssa Havlovic

Recipe of the Month: Acorn Squash Cranberry Kale Salad by Alyssa Havlovic

Winter Protection for Potted Trees and Shrubs by Sarah Browning

Garden Guide: Things to Do This Month

What's the Deal With Smelly Well Water? by Becky Schuerman

Cover Crop Grazing Conference, Nov. 1

Pantry Pests by Kait Chapman

Bedtime Blues for Young Children by Hayley Jackson

Heart of 4-H Volunteer Award: Beatrice 77 Livestock and Lon and Carol Bohling

State 4-H Archery Outdoor Results

20 Years of Heart of 4-H Award!

4-H Announcements for Enrolled …

Modification Of The Tumor Microenvironment Enhances Anti-Pd-1 Immunotherapy In Metastatic Melanoma, Guilan Shi, Megan Scott, Cathryn G. Mangiamele, Richard Heller

Bioelectrics Publications

Resistance to checkpoint-blockade treatments is a challenge in the clinic. Both primary and acquired resistance have become major obstacles, greatly limiting the long-lasting effects and wide application of blockade therapy. Many patients with metastatic melanoma eventually require further therapy. The absence of T-cell infiltration to the tumor site is a well-accepted contributor limiting immune checkpoint inhibitor efficacy. In this study, we combined intratumoral injection of plasmid IL-12 with electrotransfer and anti-PD-1 in metastatic B16F10 melanoma tumor model to increase tumor-infiltrating lymphocytes and improve therapeutic efficacy. We showed that effective anti-tumor responses required a subset of tumor-infiltrating CD8⁺ and CD4 …

Persistence And Expansion Of Cryptic Endangered Red Wolf Genomic Ancestry Along The American Gulf Coast, Bridgett M. Vonholdt, Kristin E. Brzeski, Matthew L. Aardema, Christopher J. Schell, Linda Y. Rutledge, Steven R. Fain, Amy C. Shutt, Anna Linderholm, William J. Murphy

Department of Biology Faculty Scholarship and Creative Works

Admixture and introgression play a critical role in adaptation and genetic rescue that has only recently gained a deeper appreciation. Here, we explored the geographical and genomic landscape of cryptic ancestry of the endangered red wolf that persists within the genome of a ubiquitous sister taxon, the coyote, all while the red wolf has been extinct in the wild since the early 1980s. We assessed admixture across 120,621 single nucleotiode polymorphism (SNP) loci genotyped in 293 canid genomes. We found support for increased red wolf ancestry along a west-to-east gradient across the southern United States associated with historical admixture in …

Profit-Maximizing Potassium Fertilizer Recommendations For Corn And Cotton, Kimberly B. Oliver, Michael P. Popp, Nathan A. Slaton, Gerson L. Drescher, Trenton L. Roberts

Crop, Soil and Environmental Sciences Faculty Publications and Presentations

Whereas K fertilization is necessary to maximize corn (Zea mays L.) and cotton (Gossypium hirsutum L.) yields in soils with sub-optimum K availability, maximizing yield is rarely profit-maximizing. Estimating the tradeoff between yield and fertilizer cost using current soil-building and/or yield-maximizing rate recommendations vs. profit-maximizing fertilizer-K rates (KR^*) provides insights for producers. Thirty-nine and 24 fertilizer-K rate trials were used to estimate respective corn and cotton yield response based on soil-K availability (SK). Using a field's SK, yield potential, yield response to fertilizer-K, crop price, and fertilizer-K cost, KR^* were calculated over the past 10 …

Tephritid Fruit Fly Species Composition, Seasonality, And Fruit Infestations In Two Central African Agro-Ecological Zones, Samuel Nanga Nanga, Rachid Hanna, Apollin Fotso Kuate, Komi K.M. Fiaboe, Ibrahim Nchoutnji, Michel Ndjab, Désiré Gnanvossou, Samira A. Mohamed, Sunday Ekesi, Champlain Djieto-Lordon

All Peer-Reviewed Publications

Bactrocera dorsalis and several Africa-native Ceratitis species are serious constraints to fruit production in sub-Saharan Africa. A long-term trapping and fruit collection study was conducted (2011–2016) in two contrasting agro-ecological zones (AEZs) of Cameroon to determine fruit fly species composition, seasonality, attraction to various lures and baits, and fruit infestation levels. Ten tephritid species from genera Bactrocera, Ceratitis, Dacus, and Perilampsis were captured in traps. Bactrocera dorsalis was the most dominant of the trapped species and persisted throughout the year, with peak populations in May–June. Ceratitis spp. were less abundant than B. dorsalis, with Ceratitis anonae dominating in the western …

Post-Fire Seed Dispersal Of A Wind-Dispersed Shrub Declined With Distance To Seed Source, Yet Had High Levels Of Unexplained Variation, Cara Applestein, T. Trevor Caughlin, Matthew J. Germino

Biology Faculty Publications and Presentations

Plant-population recovery across large disturbance areas is often seed-limited. An understanding of seed dispersal patterns is fundamental for determining natural-regeneration potential. However, forecasting seed dispersal rates across heterogeneous landscapes remains a challenge. Our objectives were to determine (i) the landscape patterning of post-disturbance seed dispersal, and underlying sources of variation and the scale at which they operate, and (ii) how the natural seed dispersal patterns relate to a seed augmentation strategy. Vertical seed trapping experiments were replicated across 2 years and five burned and/or managed landscapes in sagebrush steppe. Multi-scale sampling and hierarchical Bayesian models were used to determine the …

Funding Has No Effect On Clinical Outcomes Of Total Joint Arthroplasty Emerging Technologies: A Systematic Review Of Bibliometrics And Conflicts Of Interest, Garrhett G. Via, David A. Brueggeman, Joseph G. Lyons, Isabelle C. Ely, Andrew W. Froehle, Anil Krishnamurthy

School of Health and Exercise Sciences Faculty Publications

Background: The use of new total joint arthroplasty technologies, including patient-specific implants/instrumentation (PSI), computer-assisted (CA), and robotic-assisted (RA) techniques, is increasing. There is an ongoing debate regarding the value provided and potential concerns about conflicts of interest (COI).

Methods: PRISMA guidelines were followed. PubMed, MEDLINE, and Web of Science databases were searched for total hip and knee arthroplasties, unicompartmental knee arthroplasties (UKA), PSI, CA, and RA. Bibliometric data, financial COI, clinical/functional scores, and patient-reported outcomes were assessed.

Results: Eighty-seven studies were evaluated, with 35 (40.2%) including at least one author reporting COI, and 13 (14.9%) disclosing industry funding. COI and …

Biodegradable Magnesium Materials Regulate Ros-Rns Balance In Pro-Inflammatory Macrophage Environment, Maria P. Kwesiga, Amani A. Gillette, Fatemeh Razaviamri, Margaret E. Plank, Alexia L. Canull, Zachary Alesch, Weilue He, Bruce P. Lee, Roger J. Guillory Ii

Peer Reviewed Articles

The relationship between reactive oxygen and nitrogen species (ROS-RNS) secretion and the concomitant biocorrosion of degradable magnesium (Mg) materials is poorly understood. We found that Mg foils implanted short term in vivo (24 h) displayed large amounts of proinflammatory F4/80+/iNOS + macrophages at the interface. We sought to investigate the interplay between biodegrading Mg materials (98.6% Mg, AZ31 & AZ61) and macrophages (RAW 264.7) stimulated with lipopolysaccharide (RAW 264.7^LPS) to induce ROS-RNS secretion. To test how these proinflammatory ROS-RNS secreting cells interact with Mg corrosion in vitro, Mg and AZ61 discs were suspended approximately 2 mm above a …

Safety Assessment Of Novel Foods And Food Proteins, Niloofar Moghadam Maragheh

Department of Food Science and Technology: Dissertations, Theses, and Student Research

The global food market needs to grow and supply food demand to feed the growing world population. Alternative food proteins, including novel sources of safe foods and ingredients, are the candidates that could provide more environmentally sustainable choices, animal welfare, and consumers health. Novel foods and food proteins must undergo premarket safety evaluations including allergenicity assessment to reduce the risk of cross-reactivity with known allergens and uncharacterized risk to food allergic individuals. This research addressed the safety assessment of some novel foods and food ingredients using the study of stability of proteins in pepsin and sequence identity analysis in the …

Identification Of Gut Microbiome Composition Responsible For Gas Production, Erasme Mutuyemungu

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Gas production remains a significant barrier to increasing intake of dietary fiber-containing foods for many consumers. Therefore, this thesis is comprised of two studies focusing on the role of the gut microbiome in contributing to gas production during fermentation of dietary components. Previous studies have reported significant correlations between Megasphaera elsdenii and gas production during fermentation of pulses. Therefore, the objective of the first study was to determine the role of M. elsdenii in gas production by the microbiome during fermentation of foods commonly associated with gas production. Human fecal microbiomes were separated based on the presence (Me+) or absence …

Occurrence Of Hydroxyproline In Proteomes Of Higher Plants, Olivia Huffman

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Food allergies affect millions of individuals across the United States and worldwide. Peanut allergies are among the most severe food allergies because of their potentially life-threatening symptoms and lifelong persistence. Potent peanut allergen, Ara h 2, is known to contain an amino acid motif containing the posttranslational modification, hydroxyproline (HyP). HyP is associated with immunogenic response when present both in Ara h 2 and in timothy grass pollen allergen, Phl p 1. To further explore the presence of HyP in higher plants and specifically to investigate its potential presence in commonly allergenic plants, a study of 26 plant seeds was …

Lingnan Gardeners Newsletter (No. 64) = 彩園通訊 (第64期), Lingnan Gardeners, Centre For Cultural Research And Development, Lingnan University

Lingnan Gardeners Newsletter 嶺南彩園通訊

No abstract provided.

Integrated Xenosurveillance Of Loa Loa, Wuchereria Bancrofti, Mansonella Perstans And Plasmodium Falciparum Using Mosquito Carcasses And Faeces: A Pilot Study In Cameroon, Joseph Pryce, Nils Pilotte, Benjamin Menze, Allison R. Sirois, Michael Zulch, Jean Pierre Agbor, Steven A. Williams, Charles S. Wondji, Lisa Reimer

Biological Sciences: Faculty Publications

Background Community presence of loiasis must be determined before mass drug administration programmes for lymphatic filariasis and onchocerciasis can be implemented. However, taking human blood samples for loiasis surveillance is invasive and operationally challenging. A xenosurveillance approach based on the molecular screening of mosquitoes and their excreta/feces (E/F) for Loa loa DNA may provide a non-invasive method for detecting the community presence of loiasis. Methods We collected 770 wild mosquitoes during a pilot study in a known loiasis transmission area in Mbalmayo, Cameroon. Of these, 376 were preserved immediately while 394 were kept in pools to collect 36-hour E/F samples …

Financial Benchmarks For Diversified, Direct-Market Produce Farms, Michael Sciabarrasi

UNH Cooperative Extension

No abstract provided.

In-Vitro Cell Culture Model To Determine Toxic Effects Of Soil Arsenic Due To Direct Dermal Exposure, Manas Warke, Madeline English, Laura De Marchi, Rohan Deep Sarkar, Srinivas Kannan, Rupali Datta, Smitha Rao

Michigan Tech Publications, Part 1

Arsenic (As) is one of the most toxic environmental pollutants, classified as a Class I carcinogen. Anthropogenic activities have led to an increase in As contamination of soils. Using animal models to study the health impacts of As is time and cost-prohibitive, hence attempts have been made to develop in-vitro cell culture models. However, most studies so far have not represented realistic environmental exposure conditions. We exposed Human Immortalized Keratinocyte (HaCaT) and Primary Human Dermal Fibroblasts (HDFa) cells to the water-soluble fraction of arsenic extracted from As spiked Immokalee soil to study the effect of soil As on skin cells. …

Removal Of Heavy Metals From Stormwater Runoff Using Granulated Drinking Water Treatment Residuals, Viravid Na Nagara, Dibyendu Sarkar, Evert J. Elzinga, Rupali Datta

Michigan Tech Publications, Part 1

Stormwater runoff is a significant source of heavy metals, including cadmium (Cd), chromium (Cr), and nickel (Ni), which pose ecological and human health problems. Various filter media have been evaluated for heavy metal removal from stormwater via adsorption, most involving chemical- or energy-intensive processes. Aluminum-based drinking water treatment residuals (WTR), a non-hazardous byproduct of drinking water treatment, are an inexpensive sorbent for heavy metals. However, the low permeability of WTR is a problem and requires mixing with sand and carbon materials to improve flow; but such amendments also reduce its sorption capacity. To overcome this problem, a granulated WTR sorbent …

Pestfacts Wa Issue 20 - November 2022, Ciara Beard, Geoff J. Thomas, Dustin Severtson

PestFacts WA

PestFacts WA Issue 20 - November 2022 contents:

• Phomopsis can be a risk when grazing lupin stubble
• Meet PestFacts WA team member – Dusty Severtson

Haploinsufficiency Of Cyp8b1 Associates With Increased Insulin Sensitivity In Humans, Shiqi Zhong, Raphael Chèvre, David Castaño Mayan, Maria Corlianò, Blake J Cochran, Kai Ping Sem, Theo H Van Dijk, Jianhe Peng, Liang Juin Tan, Siddesh V Hartimath, Boominathan Ramasamy, Peter Cheng, Albert K Groen, Folkert Kuipers, Julian L Goggi, Chester Drum, Rob M Van Dam, Ru San Tan, Kerry-Anne Rye, Michael R Hayden, Ching-Yu Cheng, Shaji Chacko, Jason Flannick, Xueling Sim, Hong Chang Tan, Roshni R Singaraja

Children’s Nutrition Research Center Staff Publications

BACKGROUND

Cytochrome P450 family 8 subfamily B member 1 (CYP8B1) generates 12α-hydroxylated bile acids (BAs) that are associated with insulin resistance in humans.

METHODS

To determine whether reduced CYP8B1 activity improves insulin sensitivity, we sequenced CYP8B1 in individuals without diabetes and identified carriers of complete loss-of-function (CLOF) mutations utilizing functional assays.

RESULTS

Mutation carriers had lower plasma 12α-hydroxylated/non–12α-hydroxylated BA and cholic acid (CA)/chenodeoxycholic acid (CDCA) ratios compared with age-, sex-, and BMI-matched controls. During insulin clamps, hepatic glucose production was suppressed to a similar magnitude by insulin, but glucose infusion rates to maintain euglycemia were higher in mutation carriers, indicating …

Forced Forward, Timo Nurmi

The Synapse: Intercollegiate science magazine

No abstract provided.

Two Birds With One Stone: Recycling Carbon Dioxide To Produce Plastic, Sydney Rosensaft

The Synapse: Intercollegiate science magazine

No abstract provided.

Back Matter

The Synapse: Intercollegiate science magazine

No abstract provided.

Sensitive Salamanders: How Salamanders Are Impacted By Climate Change And Water Pollution, Kristen Aldridge

The Synapse: Intercollegiate science magazine

No abstract provided.

The Long Haul Virus, Sadia Haidari

The Synapse: Intercollegiate science magazine

No abstract provided.

Halluiclluistnrataed Tbyi Eovelnyn L Aozenr Medical Revolution: Using Psychedelics As A New Treatment For Disorders, Asha Shukla

The Synapse: Intercollegiate science magazine

No abstract provided.