Life Sciences Commons^™

Non-Traditional Adjuvants And Methods For Applying Root-Promoting Compounds In Commercial Cutting Propagation, Anthony Turner Bowden

Theses and Dissertations

The nursery/greenhouse industry is innovative. There have been several reports in the popular literature of non-traditional adjuvants used in plant propagation; however, these claims have never been evaluated in a scientific setting. Five studies, conducted at the South Mississippi Branch Experiment Station in Poplarville in 2019 and 2021 determined the impact that adding honey to water-soluble auxin solutions and surfactants to foliar applied auxin solutions on physiological responses associated with adventitious root formation. Treatments in studies presented in chapters two and three included three honey sources (multiflora, local, and Manuka). Honey-infused water-soluble auxin solutions affected plant species but not in …

Assessing The Legacy Of Erosion And Flood Control Management Efforts On The Fish Assemblages And Physical Conditions Of Yazoo Basin Bluff Hill Streams, Nicky M. Faucheux

Theses and Dissertations

The hills of Yazoo Basin have a long history of land use modification and subsequent erosion and flood control issues. In response, federal actions were taken to address these issues beginning after the Mississippi River flood of 1927. Four major flood control reservoirs were built in 1932-1957, and instream low-drop grade control structures (GCS) were installed beginning in the 1980s. The objective of my dissertation was to ascertain the long-term effects of these efforts on stream fish assemblages and channel morphology. To assess whether the reservoirs affected upstream fish assemblages as barriers to recolonization by fluvial fishes or as source …

Evaluation For Using Expended Bioelectrochemical Systems As Soil Amendments For Improved Corn Plant Growth And A Drought Resistant Soil., Jackson Lee Sauers

Theses and Dissertations

A long-held practice is to mix agricultural soil with a soil amendment to improve growing conditions in crops. A common soil amendment is biosolids produced from both municipal and dairy wastewater due to the macro- and micronutrients within it. Both the agricultural and wastewater industries are participating in the Circular Economy concept (CEC). Two experiments explored using expended bioelectrochemical systems (BES) that treated either synthetic dairy wastewater (DWW) or synthetic municipal wastewater (SWW) as soil amendments to improve corn plant growth when treated with three different nutrient treatments: 100%- 50%- and 0% Hoagland Nutrient Solutions. Biochar and used terracotta clay …

An Evaluation On The Effects Of Additions And Deletions Of Specific Nutrient Management Strategies On Corn Yield At Different Plant Densities, James D. Dew

Theses and Dissertations

Improved management strategies are needed to increase yield production and quality. The study aimed to determine suitable cultural practices for improved corn production. Experiments were set up at Verona and Stoneville, Mississippi, from 2020 to 2022. Treatments included row configurations (single and twin-row), plant populations (79,000 and 99,000 seeds ha-1), and six combinations of nutrients with or without fungicide. The nutrients evaluated were two nitrogen rates 235 and 314 kg N ha-1, phosphorus 45 kg P ha-1, potassium 112 kg K ha-1, sulfur 22 kg S ha-1, and zinc 11 kg Zn ha-1, and fungicide at 272 ml ha-1. In …

Healthy Eating Index And Body Fat Distribution, Masar Ozrail

Theses and Dissertations

Body fat distribution has been identified as a more significant risk factor for metabolic-related diseases. This study investigated whether body fat distribution affects dietary outcomes (quality and intake), anthropometric measures, body composition, and skin carotenoid levels. A cross-sectional study with 91 female students was conducted. Thirty-one percent of participants (n=28) had an android body fat distribution. Body fat distribution was associated with WC and WHtR (p

The Use Of 87sr/86sr Isotopic Ratios To Assess Natal Areas And Movement Of Juvenile Gulf Sturgeon Acipenser Oxyrinchus Desotoi In The Pascagoula And Pearl River Systems, Joshua Neary

Theses and Dissertations

The effects of water and dietary influence, and time duration for discernment, of pectoral fin spine ⁸⁷Sr/⁸⁶Sr were evaluated in Atlantic Sturgeon Acipenser oxyrinchus oxyrinchus. Fish were subjected to ⁸⁷Sr-enriched water (0.1 mg/L) or diet (0.25 migrogram/g or 0.5 microgram/g) for 1, 2, 3, 6, or 12 weeks. Fish were also held at 15 or 25°C to determine if temperature influenced ⁸⁷Sr/⁸⁶Sr. Results found fin spine ⁸⁷Sr/⁸⁶Sr changed in water after 1 week and diet after 12 weeks suggesting water is the primary influence. In wild Gulf Sturgeon A. o. …

Development Of A Novel Loop-Mediated Isothermal Amplification (Lamp) Assay For The Rapid Detection Of Clostridium Perfringens, Deepa Chaudhary

Theses and Dissertations

Clostridium perfringens (C. perfringens) causes diseases such as necrotic enteritis in poultry. Current methods for early detection of C. perfringens, including culture, and molecular techniques (PCR and qPCR), have logistical limitations in detecting pathogens on-site because they require specialized equipment and long sample preparation time. This study describes developing a highly sensitive on-site LAMP assay combined with a simple DNA extraction method to detect C. perfringens. Results showed that the LAMP assay was highly specific and sensitive with the optimal reaction conditions: 63°C for 30 mins with primer concentration of 1.2 mM FIP/BIP and 0.2 mM F3/B3. The HotSHOT method …

A Subspecies-Level Database Of Amazonian Bird Distributions, With Inferences On Suture Zones And Conservation, Marco Antonio Rego

LSU Doctoral Dissertations

Amazonia has the largest and most biodiverse tropical forest on the planet. However, the distributions of its species are still poorly understood, even for well-studied vertebrates such as birds. Available distribution maps for Amazonian birds often lack accuracy in delimiting the area where species occur. Existing distributional information for Amazonian birds is often limited to species-level level taxonomy, even though many Amazonian bird subspecies represent biological species. Here, I investigate the geographic distributions of 4,148 bird taxa (species and subspecies) using museum scientific specimens as the primary source of data. To build the data base I use more than 600,000 …

G-Rich Motifs Within Phosphorothioate-Based Antisense Oligonucleotides (Asos) Drive Activation Of Fxn Expression Through Indirect Effects, Feng Wang, Ezequiel Calvo-Roitberg, Julia M Rembetsy-Brown, Minggang Fang, Jacquelyn Sousa, Pranathi Meda Krishnamurthy, Michael R Green, Athma A Pai, Jonathan K Watts

Faculty, Staff and Student Publications

Friedreich's ataxia is an incurable disease caused by frataxin (FXN) protein deficiency, which is mostly induced by GAA repeat expansion in intron 1 of the FXN gene. Here, we identified antisense oligonucleotides (ASOs), complementary to two regions within the first intron of FXN pre-mRNA, which could increase FXN mRNA by ∼2-fold in patient fibroblasts. The increase in FXN mRNA was confirmed by the identification of multiple overlapping FXN-activating ASOs at each region, two independent RNA quantification assays, and normalization by multiple housekeeping genes. Experiments on cells with the ASO-binding sites deleted indicate that the ASO-induced FXN activation was driven by …

A Text-Based Smoking Cessation Intervention For Sexual And Gender Minority Groups: Protocol For A Feasibility Trial, Irene Tami-Maury, Rebecca Klaff, Allison Hussin, Nathan Grant Smith, Shine Chang, Lorraine R Reitzel, Lorien C Abroms

Faculty, Staff and Student Publications

BACKGROUND: Smoking among sexual and gender minority (SGM) groups, which include lesbian, gay, bisexual, transgender, and queer individuals, has been reported to be highly prevalent. This is attributed to several factors, including minority-specific stress and targeted tobacco marketing. Therefore, this population is at an increased risk for tobacco-related diseases. SMS text messaging programs have been found to be effective for smoking cessation and appeal to traditionally hard-to-reach populations over other interventions. It has also been suggested that targeted and tailored interventions could be more effective among SGM smokers because they can be designed to assure a safe, validating health care …

Efficacy Of Cabozantinib In Metastatic Mit Family Translocation Renal Cell Carcinomas, Jonathan Thouvenin, Omar Alhalabi, Maria Carlo, Lucia Carril-Ajuria, Laure Hirsch, Sylvie Négrier, Dylan Martini, Delphine Borchiellini, Jad Chahoud, Massimo Lodi, Philippe Barthélémy, Elshad Hasanov, Andrew W Hahn, Thierry Gil, Srinivas R Viswanathan, Ziad Bakouny, Pavlos Msaouel, Mehmet Asim Bilen, Toni K Choueiri, Laurence Albiges, Nizar M Tannir, Gabriel G Malouf

Faculty, Staff and Student Publications

Background: MiT family translocation renal cell carcinoma (TRCC) is a rare and aggressive subgroup of renal cell carcinoma harboring high expression of c-MET. While TRCC response rates to VEGF receptor tyrosine kinase inhibitors (TKIs) and immune checkpoint inhibitors are limited, efficacy of cabozantinib (a VEGFR, MET, and AXL inhibitor) in this subgroup is unclear.

Methods: We performed a multicenter, retrospective, international cohort study of patients with TRCC treated with cabozantinib. The main objectives were to estimate response rate according to RECIST 1.1 and to analyze progression-free survival (PFS) and overall survival (OS).

Results: Fifty-two patients with metastatic TRCC treated in …

Consistency Of Efficacy Results Across Various Clinical Measures And Statistical Methods In The Lecanemab Phase 2 Trial Of Early Alzheimer’S Disease, Shobha Dhadda, Michio Kanekiyo, David Li, Chad J Swanson, Michael Irizarry, Lynn D Kramer

Faculty, Staff and Student Publications

BACKGROUND: Lecanemab (BAN2401) is a humanized IgG1 monoclonal antibody that preferentially targets soluble aggregated Aβ species (protofibrils) with activity at insoluble fibrils and slowed clinical decline in an 18-month phase 2 proof-of-concept study (Study 201; ClinicalTrials.gov NCT01767311) in 856 subjects with early Alzheimer's disease (AD). In this trial, subjects were randomized to five lecanemab dose regimens or placebo. The primary efficacy endpoint was change from baseline in the Alzheimer's Disease Composite Score (ADCOMS) at 12 months with Bayesian analyses. The key secondary endpoints were ADCOMS at 18 months and Clinical Dementia Rating-Sum-of-Boxes (CDR-SB) and Alzheimer's Disease Assessment Scale-Cognitive Subscale (ADAS-Cog14) …

Inhibition Of The Phosphatidylinositol-3 Kinase Pathway Using Bimiralisib In Loss-Of-Function Notch1-Mutant Head And Neck Cancer, Faye M Johnson, Filip Janku, Mohamed A Gouda, Hai T Tran, Jitesh D Kawedia, Hendrik Streefkerk, Clark R Andersen, Defeng Deng, Seema Rawal, Pooja A Shah, Adel K El-Naggar, Jason M Johnson, Mitchell J Frederick

Faculty, Staff and Student Publications

BACKGROUND: PI3K/mTOR inhibition leads to apoptosis of NOTCH1-mutant head and neck squamous cell carcinoma (HNSCC) cells. We tested the efficacy of the PI3K/mTOR inhibitor bimiralisib in patients with NOTCH1-mutant HNSCC.

METHODS: Patients with recurrent/metastatic NOTCH1-mutant HNSCC who had progressed during chemotherapy and immunotherapy received bimiralisib until unacceptable toxicity or progression. To assess whether NOTCH1 mutations can be detected in blood, we measured circulating tumor DNA (ctDNA). To assess activated NOTCH1 protein levels, we quantitated cleaved NOTCH1 (cl-NOTCH) by immunohistochemistry.

RESULTS: Eight patients were treated, and 6 were evaluable for response. The objective response rate was 17%. For all 8 patients, …

Uncovering A Natural History Mystery At The University Of Akron, Gary M. Holliday, Lara Roketenetz

Proceedings from the Document Academy

Students were engaged in a combination of detective work, biology, archives, art, and education/outreach to prepare a collection of taxidermied birds donated to the University of Akron by the Rhodes family in the early 1900s for public access and exhibition. Students had the opportunity to learn from leading experts regarding the historical significance of biological collections, proper preservation protocols, digitizing and archival practices, and the urgency of science education and communication for a public audience. Many campus and community partners were involved, including experts from local museums and nature centers.

Performance Analysis: Using The Northern Sea Route As An Alternative To Traditional Routes, Liangkun Xu, Qing Yu

Journal of Marine Science and Technology–Taiwan

The grounding accident of M/V EVER GIVEN in the Suez Canal causes a catastrophic loss on shipping companies. Alternatively, previous studies have presented encouraging findings of the remarkable benefits of time and cost saving in the Northern sea route (NSR) due to extend the ice-free time and shorten the distance. Ships using the NSR could shorten as much as 40% of the sailing distance from China (Shanghai) to Europe (Rotterdam) compared with the traditional route via the Suez Canal. This paper investigates the economic potential of using the NSR as a cost-effective route in comparison with Suez Canal Route (SCR) …

Numerical And Approximate Analytic Solutions Of Second- Order Nonlinear Boundary Value Problems, Chein-Shan Liu, Jian-Hung Shen, Yung-Wei Chen

Journal of Marine Science and Technology–Taiwan

The shooting method consists of guessing unknown initial values, transforming a second-order nonlinear boundary value problem (BVP) to an initial value problem and integrating it to obtain the values at the right end to match the specified boundary condition, which acts as a target equation. In the shooting method, the key issue is accurately solving the target equation to obtain highly precise initial values. Due to the implicit and nonlinear property, we develop a generalized derivative-free Newton method (GDFNM) to solve the target equation, which offers very accurate initial values. Numerical examples are examined to show that the shooting method …

Optimization Of Circular Silencers Internally Hybridized With Multiple Reverse Chambers Using The Ga Method, Min-Chie Chiu, Ying-Chun Chang, Tian-Syung Lan, Ho-Sheng Chen

Journal of Marine Science and Technology–Taiwan

A noise abatement method using an eigen function in conjunction with an optimizer (Genetic Algorithm) under a space-constrain situation is proposed in this study concerning a high order wave propagation effect. An eigen function is used to describe an acoustical field and shorten the acoustical model in a four-pole transfer matrix form. The system’s four-pole matrix estimates the Transmission Loss (TL) for the silencers by multiplying individual four-pole matrices.

Additionally, a case study of an air compressor’s broadband noise elimination is introduced. Three types of circular reverse silencers (silencers A-C) internally hybridized with multiple straight chambers are adopted to efficiently …

Numerical Investigation Of The Uncertainty Of Typhoon Wave Parameters Retrieval Using Hf Radar, Toan Duy Dao, Hwa Chien

Journal of Marine Science and Technology–Taiwan

The aim of this study was to identify the sources of uncertainties of typhoon wave field monitoring using HF radar and to quantitatively assess the bias of the wave parameters, such as significant wave height and mean period, retrieved under various conditions. The strategy was to apply a purely numerical simulation of the Doppler-range spectra and then compare the estimation results to the target. For the quantitative investigation, a numerical test-bed was established. The test-bed was used to simulate the Doppler spectra based on Barrick’s (1972) theory by using the input of the directional wave spectra. The typhoon wave spectra …

A Fused Data Based Real-Time Collision Warning System For Ferries In The Yangtze River, Xiaojia Liu, Ke Zhang, Han Xue, Yingtang Li

Journal of Marine Science and Technology–Taiwan

The risks for ferries in the Yangtze River are relatively high, as they frequently cross the main traffic flows, leading to more intersections with other upwards and downwards ships. Although some studies have developed many models to assess collision risks in the Yangtze River, collision warning studies on ferries are scant. Meanwhile, most of the current collision studies evaluate risk based on AIS data, which are incapable of providing real-time ship information as they are discrete-time series data. In this work, fused data combining radar and AIS data are applied in a real-time ship collision warning model to assess the …

Experimental Study Of The Effect Of A Solid Wing Conveyor On Marine Debris Collection, Erik Sugianto, Jeng-Horng Chen

Journal of Marine Science and Technology–Taiwan

Marine debris is a global problem that has not been resolved. This has encouraged the emergence of marine debris cleaning technologies, one of which is a conveyor ship. However, how effective conveyors are in collecting waste has not been studied. In this paper, experimental research on conveyors as marine debris collectors is investigated. The capability of catamarans with or without solid wing conveyors to collect marine debris is explored. Three kinds of marine debris collection models are used: no-wing conveyor, a 12.5-cm-long wing conveyor, and an 18.75-cm-long wing conveyor. Artificial marine debris (AMD) is spread on the water surface in …

Solving Nonlinear Boundary Value Problems With Nonlinear Integral Boundary Conditions By Local And Nonlocal Boundary Shape Functions Methods, Chein-Shan Liu, Yung-Wei Chen, Jian-Hung Shen

Journal of Marine Science and Technology–Taiwan

The paper considers the second-order nonlinear boundary value problem (NBVP), which is equipped with nonlinear integral boundary conditions (BCs). Two novel iterative algorithms are developed to overcome the difficulty of NBVP with double nonlinearities involved. In the first iterative algorithm, two nonlocal shape functions incorporating the linear integral terms are derived, and a nonlocal boundary shape function (NBSF) is formulated to assist the solution. Let the solution be the NBSF so that the NBVP can be exactly transformed into an initial value problem. The new variable is a free function in the NBSF, and its initial values are given. For …

Investigating The Involvement Of The Tick Vector In The Induction Of Alpha-Galactose Hypersensitivity (Alpha-Gal Syndrome, Red Meat Allergy) In The United States., Gary Crispell

Dissertations

Alpha-gal syndrome (AGS or sometimes called red meat allergy) is a result of the development of specific IgE antibodies to the oligosaccharide galactose-α-1,3-galactose (α-gal) after a person has had exposure to tick bites. This dissertation investigates four common tick species found in North America: the lone-star tick (Amblyomma americanum), the Gulf-Coast tick (Amblyomma maculatum), the American dog tick (Dermacentor variabilis), and the black-legged tick (Ixodes scapularis) for the presence of α-gal by utilizing a combination of immunoproteomic approaches and carbohydrate analysis techniques.

Anti-α-gal IgM antibodies (M86) were used in immunoblotting to detect …

Policy Implications Of Managing Biodiversity And Natural Resources Across International Boundaries, Dillon Brown

Dissertations

Fisheries Management under the best of scenarios is a complex action. It requires thoughtful consideration of resources that tend to be out of sight, widely distributed, highly variable both spatially and temporally, and present dramatic variation in life history and ecology. No one management approach has been developed which can effectively incorporate all these variables. Add to this the issue of transnational boundary movements of these resources, and one discovers that this complex issue needs to be addressed by multiple entities, agencies, and nations to have any chance of success.

This research set out to discover ways in which fisheries …

Characterization Of Innate Immune Responses In Mouse Trophoblast Stem Cells And Their Differentiated Cells, Mona Fendereski

Dissertations

The blastocyst is the preimplantation stage embryo consisting of two major components: the inner cell mass (ICM) and the trophectoderm (TE). The ICM forms the fetus, whereas the TE contributes to the placenta development. Previous studies have demonstrated that human and mouse embryonic stem cells (ESCs) derived from the ICM are deficient in expressing type I IFNs in response to viral infection. This study investigated the innate immune responses in mouse trophoblast stem cells (TSCs) and their in vitro differentiated trophoblasts (TSC-TBs). I report that, unlike ESCs, TSCs have a functional IFN system. They can express and respond to type …

Adapting Network Theory For Spatial Network Externalities In Agriculture: A Case Study On Hemp Cross-Pollination, Jeffrey S. Young, Tanner J. Mccarty

Faculty & Staff Research and Creative Activity

Growers have increasingly expressed frustration over the negative externalities created by their neighbor's production practices. These spatial agricultural network problems include issues such as cross-pollination and herbicide drift. We develop novel methods for estimating parameters that allow us to adapt and apply general network diffusion models to these spatial agricultural network problems. Doing so allows us to calculate externality damage within a region and calculate cost-effective policies for alleviating that externality. We empirically illustrate, motivate, and test this approach by applying it to hemp. We find that network structure is an important factor in externality size and cost-effective policy response …

Effects Of Microbial Transglutaminase On Gluten-Free Sourdough Bread Structure And Loaf Characteristics, Anna J. Redd

Theses and Dissertations

In an effort to mimic the continuous protein matrix found in gluten-containing breads, the effects of the enzyme microbial transglutaminase were tested in gluten-free (GF) sourdough breads containing five different GF flours: chickpea, brown rice, white rice, oat, and quinoa. The utilization of transglutaminase in GF sourdough bread applications was shown to improve some of the final bread characteristics of GF breads. White and brown rice GF sourdough loaves at 24 hours showed a 28% and 13% decrease in crumb firmness with the addition of 2 U/g dough, respectively. Quinoa-GF sourdough breads at 24 hours showed a 6% increase in …

Deep-Brain Shape Features Differentially Relate To Social Outcomes In Pediatric Traumatic Brain Injury, Braydon Lee

Undergraduate Honors Theses

Pediatric traumatic brain injury (TBI) is a global issue with major health consequences. The integrity of brain structures and their social functional correlates are at risk in children with TBI. In this study, magnetic resonance images of the thalamus, caudate, putamen, and globus pallidus were examined in a pediatric TBI group from the Social Outcomes of Brain Injury in Kids (SOBIK). Shape deformations of these structures were compared to an orthopedically injured (OI) control. Additionally, the correlation between shape deformation and theory of mind (ToM) measures were mapped and compared. This study found inward deformations in the ventral thalamic nuclei …

Biological Remediation Of Fragipan, Corey Hale

Honors College Theses

The fragipan layer in the soil is a naturally occurring subsurface layer that restricts water percolation as well as inhibits root growth. A fragipan layer can be found almost ubiquitously across Southwestern Kentucky. This limits how agriculturalists manage soils in this area. It has been recently discovered by the University of Kentucky that Annual Ryegrass roots produce 3,4 dihydroxyphenylpropionic acid (DHPPA) that reacts with this hardpan to weaken the layer. Certain bacteria, such as Escherichia coli, Lactobacilli, and Bifidobacteria can also synthesize DHPPA through a breakdown process of chlorogenic acid. This reaction typically happens in the human digestive tract. …

The Experiences Of Children With Neurological Conditions And Families, Charmaine Alexis Pasion, Jazminne Orozco Arteaga

Fall 2022 Virtual OTD Capstone Symposium

Children with chronic neurological conditions and their families experience various challenges that impact their quality of life and occupational participation as they reintegrate back into their communities (Dumas & Grajo, 2021; Taib et al., 2021). However, rehabilitation for children with chronic neurological conditions focuses on addressing functional impairments and self-care activities rather than transitioning home (Diener et al., 2021). Therefore, children and their families are in need of resources and interventions to address their occupational impact as they return to their occupations (Diener et al., 2021). This capstone research study explored the lived experiences of children with chronic neurological conditions …

The Expanding Marine Built Environment And Biofilm Formation Processes, Rachel Mugge

Dissertations

The marine built environment (i.e., places or things made or modified by humans) in the northern Gulf of Mexico is rapidly expanding and includes over 2,000 known historic shipwrecks, nearly 4,100 artificial reefs, and other built structures for natural resource extraction, marine aquaculture, renewable energy generation, munitions dumping areas, and commercial and recreational activities. While these structures have the potential to develop into artificial reefs, acting as biodiversity hotspots that provide food and shelter and facilitate organism transport, it is unclear how they affect biodiversity and ecosystem function in the marine environment. The success of artificial reefs to become ecosystems …