Life Sciences Commons^™

Strawberry Production In An Elevated Bench Growing System Inside A High Tunnel In Southern Indiana, Wenjing Guan, Dean Haseman, Laura Ingwell, Samantha Willden, Dan Egel

Midwest Vegetable Trial Reports

Objective of this study is to evaluate the performance of eight strawberry cultivars grown in an elevated bench system. Yield and quality parameters were measured. This report also includes a discussion on production challenges and economic considerations of this production system.

First Draft Genome And Transcriptome Of Cercosporidium Personatum, Causal Agent Of Late Leaf Spot Disease Of Peanut, Renee Arias, John T. Dobbs, Jane E. Stewart, Emily G. Cantonwine, Valerie A. Omer, Victor S. Sobolev, Marshall C. Lamb, Alicia Massa

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Objective Two main fungal leaf spot diseases occur in peanut, namely early leaf spot (ELS) and late leaf spot (LLS), these cause a yearly average of $44 million losses. Limited genetic information, 3534 bp of sequencing, exists about the causal agent of LLS, Cercosporidium personatum (syn. Nothopassalora personata, syn. Phaeoisariopsis personata). The extremely slow growth of this fungus, approximately 1 cm colony in 6 months, and challenges in nucleic acid extractions have hindered research on LLS. Our goal in this work is to provide a reference genome for research on this pathogen.

Results Whole genome and transcriptome sequencing of the …

Dual Mechanisms Contributing To Pyruvate Dehydrogenase Activity Deficiency In A Barth Syndrome Cell Model, Zhuqing Liang

Wayne State University Dissertations

Barth syndrome (BTHS) is a rare genetic disease that results from mutations in the TAFAZZIN gene, which encodes the cardiolipin (CL) remodeling enzyme tafazzin (Taz). The mechanisms linking perturbation of CL remodeling and the pathological features of BTHS are not understood. We have recently reported that intermediary metabolism is perturbed in BTHS, and activity of the metabolic gatekeeper enzyme pyruvate dehydrogenase (PDH) is deficient in BTHS models. The mechanism whereby PDH is regulated by Taz is unknown, and this knowledge gap represents an obstacle to the development of therapeutics to treat BTHS. Using an established C2C12 myoblast model of BTHS, …

Regulation Of Motile Ciliogenesis And The Targeting Of Meig1/Pacrg/Dnali1 Complex For The Development Of Male Contraceptive, Yi Tian Yap

Wayne State University Dissertations

Cilia are broadly categorized into primary and motile cilia. The intraflagellar transport(IFT) system, composed of two large multimeric IFT complexes, is universally present in all cilia and facilitates bidirectional cargo transport. While the role of IFT in primary cilia and eukaryotic motile cilia has been demonstrated, the association between IFT and mammalian motile ciliogenesis remains largely unknown. Aim 1 of the present study is to uncover the role of IFT in mammalian motile ciliogenesis. By using a motile cilia-specific Ift140 knock-out mice, the present study demonstrates the significance of IFT140 in the formation and maintenance of cilia in the airways, …

Biological Adaptations Leading To Metastatic Potential In Ovarian Cancer, Hussein Chehade

Wayne State University Dissertations

Ovarian cancer induces most deaths from gynecological cancers. Patients succumb due to heavy metastatic tumor burden, which accounts for 90% of cancer related deaths and is characterized by the dissemination of malignant cells from primary tumor to secondary site(s), partaking in cancer progression. Ovarian cancer progression is an extremely demanding process that requires cancer cells to be able to survive anoikis conditions and be metabolically flexible to survive microenvironmental changes during transit to a secondary site. Mitochondrial Nuclear Retrograde Regulator 1 (MNRR1) is a bi-organellar protein primarily present in the mitochondria by which it can prevent apoptosis and/or enhance mitochondrial …

From Culture To Metagenomics: A Comprehensive Evaluation Of The Female Reproductive Tract Microbiome In Pregnancy, Jonathan Panzer

Wayne State University Dissertations

The advent of Next Generation Sequencing technologies prompted novel investigations of the microbiome. These revealed both increased diversity of known microbiomes such as those of the gut, oral cavity and vagina, but also bacterial DNA in tissues previously considered sterile such as the blood, liver, brain, and placenta. Thus, previous paradigms of sterility, based primarily on culture data, were called into question. However, interpretation of bacterial DNA signals from low-microbial biomass tissues is complex due to the strong potential for contamination and interference from the surrounding environment. Yet, many new methodologies are being developed to help isolate valid bacterial DNA …

Cardiolipin Metabolism Regulates Expression Of Muscle Transcription Factor Myod1 And Muscle Development, Linh Vo

Wayne State University Dissertations

The mitochondrial phospholipid cardiolipin (CL) is critical for numerous essential biological processes, including mitochondrial dynamics and energy metabolism. Mutations in the CL remodeling enzyme TAFAZZIN cause Barth syndrome (BTHS), a life-threatening genetic disorder that results in severe physiological defects, including cardiomyopathy, skeletal myopathy, and neutropenia. Although fatigue is among the most debilitating self-reported symptoms of BTHS patients, the mechanistic links whereby CL deficiencies lead to skeletal myopathy and defective muscle formation are not known. In this thesis, using the TAFAZZIN-knockout (TAZ-KO) mouse myoblast C2C12 model, I aimed to elucidate the mechanism of muscle differentiation defect in these cells. We carried …

Mechanistic Insights Into The Therapeutic Potential Of Adjunctive Thymosin Beta-4 Treatment In Pseudomonas Aeruginosa-Induced Keratitis, Yuxin Wang

Wayne State University Dissertations

Tβ4, a naturally occurring 43-amino acid protein, has been found to promote wound healing and reduce corneal inflammation. Topical Tβ4 as a therapeutic agent in conjunction with ciprofloxacin improves the outcome of Pseudomonas aeruginosa-induced keratitis by regulating the cellular functions of PMNs and MΦs and enhancing the production of SPMs. Understanding the mechanisms of Tβ4 provides valuable insights and novel therapeutic potential for resolving disease pathogenesis not only in the cornea but also in other infectious and immune-regulated inflammatory conditions.

Exploring The Metabolic Capabilities And Biotransformation Potential Of Environmental Lactococcus Strains, Desirée Román Naranjo

Theses

The aim of this thesis is to characterize environmental Lactococcus strains to identify different and diverse metabolic capabilities that could be applied to microbial biotransformation, defined as the conversion of a cheap/waste substrate into a value-added product. Due to the increased amounts of food processing waste generated worldwide and the impact of climate change, new sustainable approaches are necessary to contribute to waste valorization and the circular bioeconomy. We ask to what extent and in what ways environment isolated Lactococcus strains could contribute to food biotransformation by metabolizing a different range of substrates. We do so to further understand and …

Isolation And Characterisation Of Soil-Derived Bacteria With Antimicrobial Activity Against Antibiotic Resistant Pathogens, Ellen Twomey

Theses

Bacteriocins are a diverse range of ribosomally synthesized bioactive peptides, recognised predominantly for their potent antimicrobial activity against other strains closely related to the producer. The focus of this thesis was to explore this activity and examine the potential of bacteriocins to inhibit the growth of clinically relevant pathogens. Although evidence exists in the literature to suggest that bacteriocins could be applied in a similar fashion to conventional antibiotics, to date they are only utilised as preservative in food to prevent the growth of foodborne illness causing organisms. Isolating and screening bacterial strains in order to identify bacteriocin producers has …

The Use Of Membrane Filtration To Increase Native Whey Protein In Infant Formula, And Effects On Bioaccessibility And Bioactivity, Yihong Chen

Theses

Breastfeeding is recognised as the gold standard for feeding newborns and infants. However, infants who cannot be breast-fed or for whom breast milk is not available, can be fed with bovine infant milk formula (IMF) as a substitute. Researchers and producers of IMF strive to continually improve or “humanise” the composition and functionality of IMF. During production of IMF it must undergo several heat treatment steps, e.g. heat-sterilisation or spray-drying, in order to provide a safe product to the infant. These heat treatments denature the globular whey proteins in IMF. In contrast, breast milk is by its nature unheated, so …

Exercise Intensity And Fractionated Response Time, Danielle R. Martineau, Ronald V. Croce, Wayne Smith

Data Catalog

We investigated the role of three intensities of aerobic exercise (Low Intensity [LI], Moderate Intensity [MI], High Intensity [HI]) on information processing and cognition (IPC) by analyzing response time (RPT) and its fractionated components (1) reaction time (RT, interval between stimulus onset and muscle activation, and (2) movement time (MT, time interval between muscle activation and completion of the required motor response). Twenty-seven participants (16, male; 11, female) between the ages of 18-25 (mean age = 21.9 years) took part in 1-choice, 5-choice, and dual-task (DT, counting backwards by 3) conditions prior to and 1-min and 20-min postexercise. Based on …

Study Of Legionella Pneumophila Treatment With Copper In Drinking Water By Single Cell-Icp-Ms, Lei Xu, Austin Sigler, Anna Chernatynskaya, Lindsey Rasmussen, Jingrang Lu, Endalkachew Sahle-Demessie, David J. Westenberg, Hu Yang, Honglan Shi

Biological Sciences Faculty Research & Creative Works

Legionella pneumophila is a persistent opportunistic pathogen that poses a significant threat to domestic water systems. Previous studies suggest that copper (Cu) is an effective antimicrobial in water systems. A rapid and sensitive quantification method is desired to optimize the conditions of L. pneumophila treatment by Cu and to better understand the interaction mechanisms between Cu and cells. In this study, we developed a highly sensitive single cell (SC)-ICP-MS method to monitor L. pneumophila cell concentration and track their uptake of Cu. The SC-ICP-MS method showed excellent sensitivity (with a cell concentration detection limit of 1000 cells/mL), accuracy (good agreement …

Exploring Population Structure And Diversity Across Time In Population Of American Flamingos (Phoenicopterus Ruber), Jessica Folsom

Electronic Theses and Dissertations, 2020-2023

Dispersal and connectivity within a population often promotes overall diversity and adaptive potential, therefore a disruption of existing patterns of connectivity by local extinction can have significant consequences for the affected species. However, the extent of these impacts on recovery is not always known. American flamingos (Phoenicopterus ruber) present an ideal opportunity to investigate how changes in gene flow influence within population diversity and genetic structure over the span of a century for a recovering population. Flamingos were highly sought-after by hunters for their meat and feathers, and as a result, the Florida population was extirpated by the beginning of …

Acute Effects Of Sprint Interval Training And Blood Flow Restriction On Neuromuscular And Muscle Function, David Gonzalez Rojas

Electronic Theses and Dissertations, 2020-2023

The purpose of this investigation was to examine the effects of continuous (CBFR) and intermittent (IBFR) blood flow restriction (BFR) applied during sprint interval training (SIT) on performance, muscle, and neuromuscular function. Fifteen men completed SIT with CBFR, IBFR, and No-BFR. Each SIT bout consisted of 2, 30-s maximal sprints on a cycle ergometer with a resistance of 7.5% of body mass. Concentric peak torque (CPT), maximal voluntary isometric contraction (MVIC) torque, and muscle thickness (MT) were measured before and after the SIT protocols during each visit. During the maximal strength assessments, surface electromyography (sEMG) was recorded and during each …

Food Waste Storage Gaseous Emissions Detection And Quantification Using Infrared Spectroscopy, Ryley A. Burton-Tauzer

Cal Poly Humboldt theses and projects

A growing interest in sustainable waste management and the implementation of new policies have prompted a shift towards alternative resource recovery methods for organic waste, including food waste. To effectively assess alternative food waste treatment scenarios, it is important to evaluate the life cycle impacts associated with each scenario. The storage phase of food waste, encompassing its accumulation in kitchens, and storage in bins for collection and transportation, has been overlooked as a source of greenhouse gases in previous studies. This investigation aimed to identify the greenhouse gases emitted during the initial five-day period of low-oxygen storage. An open dynamic …

Oxidative Stress-Related Biomarkers As Forensic Biomarkers For Burned Skin Vitality In Rats, Ola A. Habotta, Ahmed Ateya, Ahmed Abdeen, Iman Ibrahim, Mohamed M. Badawy, Ahmed M. Zeina

Mansoura Veterinary Medical Journal

Objective: In forensic practice, the diagnosis of whether a skin injury is inflicted in life or not is a challenging research topic. Therefore, new reliable biomarkers of vitality are urgently required. In this study, biochemical, molecular and histopathological techniques were used to explore the role of oxidative stress biomarkers in differentiating antemortem from postmortem burn injury. Design, animals and procedures: Eighteen male rats were allocated to three groups: normal unburnt group, antemortem burn group and postmortem burn group. Results: The analysis of skin specimens revealed a notable increase in MDA levels together with a decrease in GSH and TAC in …

An Overview Of Meat Composition, Postmortem Changes, And Sources Of Microbial Contamination In Developing Countries Abattoirs, Hend Ali Elshebrawy, Nahed Gomaa Kasem, Khalid Ibrahim Sallam

Mansoura Veterinary Medical Journal

Meat is an essential component of a healthy and well-balanced diet and has played a crucial role in human evolution over the years because it is an excellent source of highly digested protein, vitamin B12, vitamin B6, niacin, iron, copper, zinc, and selenium. Raw meat is considered an unavoidable source of microbial contamination due to its enriched nutrient composition, high pH (5.5 to 6.5), and water activity (0.98 to 0.99); consequently, the meat is regarded as a perfect medium for bacterial growth. Meat deterioration begins immediately after slaughter because of faulty practices during the processing stages such as skinning, evisceration, …

Bacterial Pneumonia Associated With Multidrug-Resistant Gram-Negative Pathogens: Understanding Epidemiology, Resistance Patterns, And Implications With Covid-19, Dalal Hammoudi Halat, Carole Ayoub Moubareck

All Works

The ongoing spread of antimicrobial resistance has complicated the treatment of bacterial hospital-acquired pneumonia (HAP) and ventilator-associated pneumonia (VAP). Gram-negative pathogens, especially those with multidrug-resistant profiles, including Escherichia coli, Klebsiella pneumoniae, Enterobacter spp., Pseudomonas aeruginosa, and Acinetobacter spp., are an important culprit in this type of infections. Understanding the determinants of resistance in pathogens causing pneumonia is ultimately stressing, especially in the shadows of the COVID-19 pandemic, when bacterial lung infections are considered a top priority that has become urgent to revise. Globally, the increasing prevalence of these pathogens in respiratory samples represents a significant infection challenge, with major limitations …

Time Of Day Effects On Maximal Effort Strength/Power And Fatigability Assessments, Justine Renziehausen

Electronic Theses and Dissertations, 2020-2023

The circadian rhythm is a 24-hour cycle present within most biological systems that is controlled by the suprachiasmatic nucleus (SCN) of the hypothalamus. The SCN is responsible for synchronizing the peripheral "clocks" present in other tissues so that all rhythms, such as heart rate, body temperature, and hormone secretion, are functioning together. As most systems experience a circadian rhythm, physical performance likely does as well. Previous studies have investigated time of day effects on maximal strength, power, and endurance performance, however, factors (e.g., sex, chronotype, age, etc.) have not clearly been distinguished. Additionally, body temperature has been suggested to be …

A Novel Ruthenium-Silver Based Antimicrobial Potentiates Aminoglycoside Activity Against Pseudomonas Aeruginosa, Gracious Yoofi Donkor, Greg M. Anderson, Michael Stadler, Patrick Ofori Tawiah, Carl D. Orellano, Kevin A. Edwards, Jan-Ulrik Dahl

Faculty Publications – Biological Sciences

The rapid dissemination of antibiotic resistance combined with the decline in the discovery of novel antibiotics represents a major challenge for infectious disease control that can only be mitigated by investments in novel treatment strategies. Alternative antimicrobials, including silver, have regained interest due to their diverse mechanisms of inhibiting microbial growth. One such example is AGXX, a broad-spec­trum antimicrobial that produces highly cytotoxic reactive oxygen species (ROS) to inflict extensive macromolecular damage. Due to the connections identified between ROS production and antibiotic lethality, we hypothesized that AGXX could potentially increase the activity of conventional antibiotics. Using the gram-negative pathogen Pseudomonas …

Advancing Methods Of Diet Analysis: A Case Study Using Degraded Merlin (Falco Columbarius) Prey Remains, Taylor A. Coon

Undergraduate Theses, Professional Papers, and Capstone Artifacts

Prey remains have long been used as a mechanism to approach diet analyses. As understanding diet is key to comprehending ecosystem dynamics, prey remains identification requires a unique methodological approach to determine diversity within a sample. With the advancement of technology, molecular protocols designed for species-specific identification have improved to incredible accuracy and precision. Yet, the visual identification method has remained a predominant technique within diet studies. With entry-level observers, we matched visual identifications with molecular-based methods to quantify the accuracy of the visual identification method. This study determined what fraction of visually identified prey remains could be correctly identified …

Assessing The Efficacy Of Beaver Dam Analogs In Willow Restoration, Hannah Hill, Dylan Ritter

Undergraduate Theses, Professional Papers, and Capstone Artifacts

Willow establishment is a necessary objective of stream restoration due to their role in bank stabilization, stream shading, and enhancement of biodiversity across the riparian zone. However, anecdotal observation indicated that establishment on beaver dam analogue (BDA) restored streams may be lacking. BDA’s mimic the pooling effects of natural beaver structures and are intended to help recreate historic conditions that existed before beaver extirpation. When working as intended, ecosystem function is restored, and a major aspect of this is willow presence. Because of these observations, as well as limited information on willow recruitment following restoration, we ask the following questions. …

Effects Of Warming And Drying On The Survival And Performance Of Giant Salmonfly Nymphs (Pteronarcys Californica), Kailie Todd

Undergraduate Theses, Professional Papers, and Capstone Artifacts

Rising temperatures and anthropogenic influences threaten to alter aquatic ecosystems as oxygen supply and demand is altered depending on temperature, flow, and water presence. In late November of 2021, a dam malfunction occurred on the Madison River in Montana that caused the river to dry rapidly downstream of the dam and reduced flow by 70% for 50 hours. My experiments were designed to determine the effects of this event by assessing how long giant salmonfly nymphs (Pteronarcys californica) can survive in still water and in air depending on temperature and relative humidity. In the laboratory, we exposed salmonfly …

Out Of The Frying Pan And Into The Fire: How Hunting Affects The Diel Activity Patterns Of Ungulates In Lubrecht Experimental Forest, Shawn M. Parsons

Undergraduate Theses, Professional Papers, and Capstone Artifacts

Predator-prey dynamics shape diel activity patterns of large mammals. The avoidance of predation risk by prey in both space and time can affect predator-prey overlap and reduce risk of mortality. Here, I examined if rifle hunting affected the diel activity patterns of ungulates and their primary predator, pumas, in Lubrecht Experimental Forest, Montana. I deployed 30 infrared wildlife remote cameras across 29 sites using a stratified random design over three months before, during, and after the Fall 2022 Montana rifle hunting season. This project was also part of the Snapshot USA 2022 initiative. Using timestamps from these photos, daily activity …

Food Fan Academy: Boosting Informal Educators Abilities To Use Food As A Teaching Tool, Kelly Furr

Graduate Research Theses & Dissertations

Introduction: Food, fitness, and nutrition (FAN) professional development (PD) for informal educators can serve to impact science, technology, engineering, art, mathematics, and medicine (STEAM+M) learning environments. Using food as a teaching tool in shaping the development of informal educators and education of students offers a unique opportunity for the acquisition of scientific knowledge and essential skills. The purpose of this study was to develop a Food FAN Academy for informal educators and evaluate the effectiveness of the PD. Methods: Case study method was utilized for the research study and the Food FAN Academy PD was a two-hour session designed in …

Characterizing Vhh Domains As Stabilization And Oligomeric Control Modules, Niyati Patel

Graduate Research Theses & Dissertations

The biophysical properties of the VHH domain from heavy-chain-only antibodies obtained from the Camelidae family has expanded opportunities for novel antibody applications. The small, single domain nature of VHH domains makes them attractive modules for fusion proteins. Here, two VHH fusion systems were evaluated, RNase A-anti-RNase A VHH and GFPMut3aR80Q-anti-GFP VHH, to investigate how antigen-VHH fusions may enhance protein thermostability. The introduction of various length flexible linkers was hypothesized to play a role in stability, as well as influence the oligomeric state. Bovine Pancreatic Ribonuclease A, or RNase A, is an enzyme belonging to the ribonuclease A superfamily, which is …

Effectiveness Of The Catch (Coordinated Approach To Child’S Health) Rainbow Program In Elementary Schools For Change In Fruit And Vegetable Intake, Ashley M. Valinskas

Graduate Research Theses & Dissertations

Background: There is currently a lack of nutrition education programs that effectively increase fruit and vegetable (FV) intake among children. Not meeting the recommendations for fruits and vegetables increases the risk for poor academic achievement, obesity, and chronic diseases such as cardiovascular disease, type II diabetes, asthma, and more. Nutrition, cooking, and gardening lessons individually and together have shown to increase fruit and vegetable (FV) consumption in school-aged children.Methods: Two-Hundred Twenty-five 4th and 5th graders participated in six cooking and six gardening lessons between September 2021 and May 2022 at Genoa Elementary school. Each nutrition education session was 25-minutes long, …

Wa Insecticide Guide 2023 - Autumn Winter, Svetlana Micic, Alan Lord

PestFacts WA

Registered chemicals for broadacre crops in Western Australia.

The pesticides listed in the tables within this guide can be used on any crop appearing on the chemical label, if the rate used does not exceed the highest rate that is registered for use on that crop.

There are many products with different trade names that contain the same active ingredient. This list is not exhaustive and does not imply any specific recommendations of brand names.

Unless otherwise specified all insecticides listed are emulsifiable concentrate (EC). Insecticides with SC are suspension concentrate, WP is wettable powder, CS is capsule suspension, WG …

Role Of Micrornas In Intestinal Inflammation And Barrier Homeostasis After Alcohol And Burn Injury, Caroline J. Herrnreiter

Dissertations

MicroRNAs are small noncoding RNA molecules that negatively regulate gene expression. Within the intestinal epithelium, miRNAs play a critical role in gut homeostasis and aberrant miRNA expression has been implicated in various disorders associated with intestinal inflammation and barrier disruption. In this study, we sought to profile changes in intestinal epithelial cell miRNA expression after alcohol and burn injury and elucidate their impact on inflammation and barrier integrity. In a more targeted approach, we began by focusing on anti-inflammatory miRNAs that, when downregulated, could exacerbate inflammation and result in intestinal barrier disruption. Using a mouse model of acute ethanol intoxication …