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Articles 44791 - 44820 of 255951
Full-Text Articles in Life Sciences
Levels Of Autotrophy And Heterotrophy In Mesophotic Corals Near The End Photic Zone, Amy Carmignani, Veronica Z. Radice, Kathryn M. Mcmahon, Alex I. Holman, Karen Miller, Kliti Grice, Zoe Richards
Levels Of Autotrophy And Heterotrophy In Mesophotic Corals Near The End Photic Zone, Amy Carmignani, Veronica Z. Radice, Kathryn M. Mcmahon, Alex I. Holman, Karen Miller, Kliti Grice, Zoe Richards
Biological Sciences Faculty Publications
Mesophotic corals live at ~30-150 m depth and can sustain metabolic processes under light-limited conditions by enhancing autotrophy through specialized photoadaptations or increasing heterotrophic nutrient acquisition. These acclimatory processes are often species-specific, however mesophotic ecosystems are largely unexplored and acclimation limits for most species are unknown. This study examined mesophotic coral ecosystems using a remotely operated vehicle (Ashmore Reef, Western Australia at 40–75m depth) to investigate the trophic ecology of five species of scleractinian coral (from genera Leptoseris, Pachyseris, and Craterastrea) using stable isotope analyses (δ13C and δ15N) of host and symbiont tissues …
Mass Spectrometry-Based Proteomic Analysis To Characterise Barley Breeding Lines, Mahya Bahmani
Mass Spectrometry-Based Proteomic Analysis To Characterise Barley Breeding Lines, Mahya Bahmani
Theses: Doctorates and Masters
Barley is a key ingredient in the malting and brewing industry, and it is the fourth most important crop being cultivated worldwide. The protein content of the barley grain is one of the main components determining the quality and nutritive value of the food and beverages prepared from barley. Mass spectrometry-based proteomic analysis is a valuable tool that can guide and inform plant breeding strategies and crop improvement programs. Understanding the proteome changes in barley grain under different growing locations, the impact of different environmental conditions and its relationship with malting characteristics have the potential to inform breeding programs to …
Potential Anti-Alzheimer Properties Of Mogrosides In Vitamin B12-Deficient Caenorhabditis Elegans, Denia Cai Shi, Chunlin Long, Ella Vardeman, Edward J. Kennelly, Michael A. Lawton, Rong Di
Potential Anti-Alzheimer Properties Of Mogrosides In Vitamin B12-Deficient Caenorhabditis Elegans, Denia Cai Shi, Chunlin Long, Ella Vardeman, Edward J. Kennelly, Michael A. Lawton, Rong Di
Publications and Research
Vitamin B12 deficiency can lead to oxidative stress, which is known to be involved in neurodegenerative diseases such as Alzheimer’s disease (AD). Mogrosides are plant-derived triterpene glycosides that exhibit anti-inflammatory and antioxidant activity in animal cell lines and mouse models. Since amyloid-β toxicity is known to cause oxidative stress and damage to brain cells, we hypothesized that mogrosides may have a protective effect against AD. In this study, we investigated the potential anti-AD effect of mogrosides in vitamin B12-deficient wild-type N2 and in transgenic CL2355 Caenorhabditis elegans expressing amyloid-β peptide. Our data indicated that mogrosides have a beneficial effect on …
Developmental Consequences Of Hatching Asynchrony In Boat-Tailed Grackles (Quiscalus Major), Emily Radner
Developmental Consequences Of Hatching Asynchrony In Boat-Tailed Grackles (Quiscalus Major), Emily Radner
Scripps Senior Theses
Boat-tailed grackles (Quiscalus major) typically lay clutches of three eggs and begin incubation before the clutch is complete, resulting in hatching asynchrony. To study the developmental consequences of hatching asynchrony on boat-tailed grackles, data loggers measured nest temperatures and were compared to ambient temperatures to see when incubation began, and it appeared to begin after the second egg is laid. The third laid egg had a significantly shorter lay to pip time and lay to hatch time than the first laid egg, highlighting the need to investigate the consequences of a shortened incubation time on development. To do …
Utilizing Amr Surveillance Data To Analyze Trends In Escherichia Coli Antibiotic Resistance Levels In The South African Provinces Of Gauteng And The Eastern Cape, Celia Maris
Scripps Senior Theses
Antimicrobial resistant bacterial pathogens pose an ongoing threat to universal public health by causing more severe manifestations of infectious diseases, leading to an increase in healthcare expenditures and higher global mortality rates. Therefore, developing strategies to combat bacterial antimicrobial resistance (AMR) is vital for the future of human health. One promising strategy is through the utilization of AMR surveillance programs. This study focused on using AMR surveillance data from South Africa to analyze changes in Escherichia coli resistance levels to numerous antibiotics between January 2012 and December 2022 in Gauteng Province and the Eastern Cape Province. The study aimed to …
Expression Of Htyr In Drosophila As A Novel Model Of Parkinson’S Disease, Madeleine Callan
Expression Of Htyr In Drosophila As A Novel Model Of Parkinson’S Disease, Madeleine Callan
Scripps Senior Theses
Parkinson’s disease is a debilitating and often deadly neurodegenerative disease affecting a growing and large population. Its etiology has long remained elusive, and because no other organisms have Parkinsonian-like diseases, it is difficult to study PD using model organisms. Neuromelanin (NM), an insoluble melanin synthesized in the dopaminergic synthesis pathway in DA neurons, has recently been implicated in PD as a major causal factor. At high levels in DA lysosomes, it functions as a proteostatic pathway inhibitor–blocking dopaminergic neurons from breaking down harmful molecules until the lysosomes eventually degenerate as well as triggering autophagy, inflammation, and total neurodegeneration. Recently, neuromelanin …
Baba Yaga: An Ecofeminist Analysis Of The Witch Of The Woods, Maya Lozinsky
Baba Yaga: An Ecofeminist Analysis Of The Witch Of The Woods, Maya Lozinsky
Scripps Senior Theses
In this thesis, I will argue that Baba Yaga’s prevalence in Russia’s culture and media provide a unique opportunity to gain insight into the junctures between the climate crisis and gender inequality in Russia. Despite the persistent gender inequities present in current Russian society, ecofeminist frameworks and ideologies are already deeply embedded in Russian culture. Women, as a group, have always been politically active in Russia, from resisting the introduction of Christianity in the 9th century, to the feminist resistance group Pussy Riot founded in 2011. I will examine Baba Yaga’s history, her role in the Russian folktale, and her …
Variation In Soil-Mediated Drought Response Traits Between Sympatric Species Of Mimulus, Anna Reitman
Variation In Soil-Mediated Drought Response Traits Between Sympatric Species Of Mimulus, Anna Reitman
Scripps Senior Theses
The ‘time limitation hypothesis’ is an evolutionary theory regarding evolution in angiosperms. The theory attributes ephemeral drought stress to transitions from a perennial to annual life history and an animal- to self-pollinating mating style. The most influential edaphic parameter on water availability is soil texture (i.e. grain size) due to its controls on porosity. Adhesion weakens with coarseness allowing for expedited rates of water loss via evaporation and percolation. The potential implications of coarse soil textures on major transitions in angiosperms due to drought mediation as the ‘time-limitation hypothesis’ proposes has yet to be thoroughly examined. Here, we grew two …
Oral Contraceptives And Affective Disorders: Neurobiology And Informed Choice, Sophia Mae Drezner
Oral Contraceptives And Affective Disorders: Neurobiology And Informed Choice, Sophia Mae Drezner
Scripps Senior Theses
Pregnancy prevention and female reproductive freedom have been some of the most contested political issues for decades. Abortion, a fundamental part of women’s healthcare, divides liberals and conservatives on an international scale. The consequences of unintended pregnancy without safe and reliable contraception are widespread, disproportionately impacting women of color, trans and non-binary folks, and poorer communities. The birth control pill is the most common form of oral contraception (OC) globally. Many people with ovaries begin the pill or other hormonal contraceptive (HC) methods as young as 11 years old. Exogenous progesterone and estrogen are known to impact mood, affect, physiology, …
Interpersonal Emotions As Emergent Phenomena: Social Neuroscience Beyond Western Cultural Constructions, Kaitlyn Penchina
Interpersonal Emotions As Emergent Phenomena: Social Neuroscience Beyond Western Cultural Constructions, Kaitlyn Penchina
Scripps Senior Theses
Because science as it exists today is a cultural construction of the West, studies of neuroscience have often been limited by Western perspectives. In particular, the Western proclivity towards individualism has led to a field of neuroscience which has historically focused on studying single individuals, as opposed to social or collective neuroscience. For the most part, it has just been assumed that collective phenomena such as interpersonal emotions must be able to be reduced in terms of individual phenomena such as individual emotions. However, closer review reveals that interpersonal emotions have emergent properties that individual emotions alone do not account …
Concrete Everywhere: A Project-Based Analysis Of The Unequal Distribution Of Warehouses In Fontana, Chanah Haigh
Concrete Everywhere: A Project-Based Analysis Of The Unequal Distribution Of Warehouses In Fontana, Chanah Haigh
Scripps Senior Theses
Unjust legislation and zoning practices drive the excess of warehouses in Fontana, California whose impacts fall disproportionately on low income communities of color. This paper addresses the environmental and health impacts of warehouses, as well as the legislation and the use of zoning which bring them to Fontana. Original maps show the spread of warehouses over census tract level data depicting race, income, and environmental factors such as diesel particulate matter. State and local legislation is analyzed to determine its intended effect and how well it has been enforced. Research was conducted through a project based thesis done in conjunction …
Location Of The Fibronectin Binding Domain On The Fibronectin Attachment Protein Of Mycobacterium Avium Subsp. Paratuberculosis, Chrissy Rose Hattery
Location Of The Fibronectin Binding Domain On The Fibronectin Attachment Protein Of Mycobacterium Avium Subsp. Paratuberculosis, Chrissy Rose Hattery
All Graduate Theses, Dissertations, and Other Capstone Projects
Mycobacterium avium paratuberculosis (MAP) is an acid-fast bacillus that causes a fatal granulomatous ileocolitis called Johne’s Disease that affects primarily ruminants. The impact of MAP infection in the US dairy industry is particularly severe, with up to 70.4% of the herds being infected and annual losses as high as $1.5 billion. MAP is capable of persisting for months in the livestock environment and surviving the pasteurization process. While several vaccines are available, none can prevent infection and treatment with antimycobacterial chemotherapy does not result in a cure. Management and control of MAP infection requires characterization of host-pathogen interaction at the …
Investigating Mirna Regulation Of The Human Apobec3 Enzymes, William Dietrich
Investigating Mirna Regulation Of The Human Apobec3 Enzymes, William Dietrich
All Graduate Theses, Dissertations, and Other Capstone Projects
The human apolipoprotein B mRNA editing enzyme catalytic polypeptide 3 (APOBEC3, A3) are a family of proteins consisting of seven enzymes, A3A, B, C, D, F, G and H, which function as cytosine deaminases. The enzymes’ purpose in the cell is to mutate viral DNA during infection hindering or stopping replication of viruses such as human papillomavirus, herpes simplex virus, and HIV-1. Several of the A3 enzymes have also been implicated in contributing to cancers such as head and neck and breast cancers by mutating cellular genomic DNA, making the ability to control A3 expression an attractive target for cancer …
Novel Amino Acids Dictate Differences In Sivmac239 And Hiv-2 Rod9 Vpx Cellular Localization, Hunter D. Mayhew
Novel Amino Acids Dictate Differences In Sivmac239 And Hiv-2 Rod9 Vpx Cellular Localization, Hunter D. Mayhew
All Graduate Theses, Dissertations, and Other Capstone Projects
Vpx is a protein encoded by Human Immunodeficiency Virus Type 2 (HIV-2) and specific Simian Immunodeficiency Viruses (SIVs) that is essential for successful infection of non-dividing myeloid cells. Vpx facilitates viral replication by trafficking the viral genome into the nucleus of infected cells and targeting the primarily nuclear innate immune protein SAMHD1 for proteasomal degradation. Considering that these activities occur at the nucleus, we hypothesized that Vpx nuclear localization would be tightly correlated with these functions. To assess this, site directed mutagenesis in combination with confocal microscopy or Western Blotting analyses were utilized to evaluate the determinants of nuclear accumulation …
The Role Of Insulin-Like Growth Factor I In The Development Of The Cerebellum In Euthyroid And Hypothyroid Mice, Prathibha Mangedarage
The Role Of Insulin-Like Growth Factor I In The Development Of The Cerebellum In Euthyroid And Hypothyroid Mice, Prathibha Mangedarage
All Graduate Theses, Dissertations, and Other Capstone Projects
Thyroid hormone deficiency during development is known to cause anatomical defects in the cerebellum that lead to neurological deficits. It has been observed that insufficient thyroid hormone levels also decrease the levels of brain derived Insulin-like growth factor 1 (Igf-1). Given that the deficits caused by low Igf-1 levels are similar to those caused by low thyroid hormone, we designed this study to investigate if the deficits caused in the development of the cerebellum by low thyroid hormone levels may be due to the lack of sufficient Igf-1. We also sought to determine if ectopic over expression of Igf-1 in …
"Effects Of Chronic Clothianidin Exposure On Various Aspects Of Oncorhynchus Mykiss (Rainbow Trout) Development", Jacob Schmid
"Effects Of Chronic Clothianidin Exposure On Various Aspects Of Oncorhynchus Mykiss (Rainbow Trout) Development", Jacob Schmid
All Graduate Theses, Dissertations, and Other Capstone Projects
Agricultural run-off is one of the leading sources of environmental pollution in the United States. One major pollutant within this is insecticides, and among these, neonicotinoids which have become the most used insecticide class across the world. Neonicotinoids act as an agonist on the nicotinic acetylcholine receptor (nAChR) in the central nervous system of insects. Neonicotinoids are perceived to have low toxicity on non-target organisms however recently extensive literature has been published showing various effects on non-target organisms. Clothianidin is one of the most applied compounds belonging to the neonicotinoid class, yet few studies have investigated its chronic effects on …
Environmental Heterogeneity As A Driver Of Understory Vegetation Composition In Midwestern Oak Savannas, Jean Pengra
Environmental Heterogeneity As A Driver Of Understory Vegetation Composition In Midwestern Oak Savannas, Jean Pengra
All Graduate Theses, Dissertations, and Other Capstone Projects
The following thesis is submitted as two chapters. Chapter one contains background information, literature review, and predictions. Chapter two is formatted as a paper submission to Invasive Plant Science and Management. Note that this journal requires a combined results and discussion section and a summary of management implications written after the abstract. The appendix contains supplementary materials that are relevant to study hypotheses but do not necessarily align with the scope of the article submission. Oak (Quercus spp.) savannas are one of the most threatened ecological systems within the United States. Like many ecotones, these savannas are considered biodiversity hotspots …
Seasonal Resource Allocation And Accumulated Degree Day Estimation For Cuban Bulrush (Oxycarum Cubense) In The Southeastern U.S., Allison Squires
Seasonal Resource Allocation And Accumulated Degree Day Estimation For Cuban Bulrush (Oxycarum Cubense) In The Southeastern U.S., Allison Squires
All Graduate Theses, Dissertations, and Other Capstone Projects
Cuban bulrush (Oxycaryum cubense (Poepp. & Kunth) Lye) is a non-native aquatic plant from South America and the West Indies, first introduced into the United States in 1878. This study focused on the resource allocation and accumulated degree day modeling of the monocephalous and polycephalous Cuban bulrush populations in Mississippi, Louisiana, and Florida. Environmental factors such as photoperiod and temperature were analyzed on its effect on plant growth. From October 2021- September 2022 Cuban bulrush biomass was harvested monthly from two 0.04 Ha (0.1 ac) plots in each state. Height was measured during each monthly event. Environmental monitoring of photoperiod …
Investigation Of Glycoside Hydrolases And Calcium In Cytophaga Hutchinsonii Cellulose Utilization, Choua Kou Vang
Investigation Of Glycoside Hydrolases And Calcium In Cytophaga Hutchinsonii Cellulose Utilization, Choua Kou Vang
All Graduate Theses, Dissertations, and Other Capstone Projects
Cytophaga hutchinsonii is a soil bacterium that can utilize cellulose as a carbon source. The cellulose utilization system of C. hutchinsonii has been predicted to be novel and its endoglucanases could potentially be used in the production of cellulosic based biofuels. These enzymes could be used to process cellulose to glucose or other soluble sugars, which can be further converted to ethanol by yeast fermentation. Current biofuels utilize starches, primarily from corn, which has direct competition with farming space for food crops. This study focuses on observing the growth of C. hutchinsonii knockout mutant strains on cellulose substrate to better …
Evaluation Of Autonomous Recording Unit Performance In Detecting Black-Billed Cuckoos, Jenna L. Asnault
Evaluation Of Autonomous Recording Unit Performance In Detecting Black-Billed Cuckoos, Jenna L. Asnault
Undergraduate Theses, Professional Papers, and Capstone Artifacts
Black-billed Cuckoos (Coccyzus erythropthalmus) are classified as a species of concern in Montana and have declined due to habitat loss and fragmentation. Cuckoos are challenging to study, as they do not move or call frequently in the presence of people. This makes researching and managing these birds difficult. Autonomous acoustic survey methods offer the potential to be more effective than traditional avian survey methods. Autonomous recording units (ARUs) are small, programmable, relatively inexpensive acoustic detectors, and have been used in several other studies. Cuckoos, however, nest and perch in dense riparian vegetation, potentially limiting the detection capabilities of …
The Links To Cancer: How Golf Became Dangerous And What We Can Do To Save The Game, Meredith Boos
The Links To Cancer: How Golf Became Dangerous And What We Can Do To Save The Game, Meredith Boos
Undergraduate Theses, Professional Papers, and Capstone Artifacts
This study is a comprehensive meta-analysis on health claims linked to exposure to golf courses, more specifically the chemicals used to maintain their appearance. It provides a brief history of the golf industry and how its growth exacerbated the environmental impact as well as an explanation of the legal landscape that will affect golf course management. Golf courses can disrupt local ecologies, contaminate ground water, rivers, lakes and streams with run-off, and be responsible for the bioaccumulation of chemicals which remain dangerous for decades. Despite the adverse effects of golf courses on the environment, there remains an opportunity to transform …
[Snakehead (Channa Marulius)]Neurocranium, Sam Gorman, Jessica Rutenis, David W. Kerstetter
[Snakehead (Channa Marulius)]Neurocranium, Sam Gorman, Jessica Rutenis, David W. Kerstetter
All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series
Snakehead Neurocranium
The Anatomy And Phylogeny Of A New Large Plioplatecarpine Mosasaur From The Campanian Bearpaw Shale Of Montana (Usa), Richard A. Carr
The Anatomy And Phylogeny Of A New Large Plioplatecarpine Mosasaur From The Campanian Bearpaw Shale Of Montana (Usa), Richard A. Carr
Master's Theses or Doctor of Nursing Practice
In 2018, a large and associated plioplatecarpine mosasaur skull, pectoral girdle, and rib cage, whose total body length may have exceeded five meters, was uncovered in the Late Campanian Bearpaw Shale of Northeast Montana (USA). Phylogenetic analysis of this specimen, MOR 10855, recovers this individual as a basal member of the genus Plioplatecarpus. This specimen, is unique in that it is estimated to be nearly twice the size of any of the other species of Plioplatecarpus found in the Western Interior Seaway during this part of the Cretaceous. While the included phylogenetic study suggests MOR 10855 represents a new …
Evaluation Of Precision Ingredient Inclusion On Production Efficiency Responses In Finishing Beef Cattle, Santana R. Hanson
Evaluation Of Precision Ingredient Inclusion On Production Efficiency Responses In Finishing Beef Cattle, Santana R. Hanson
Electronic Theses and Dissertations
Two randomized complete block design feedlot experiments were conducted over the course of two years. These experiments were conducted to investigate the effects of 1) evaluate the observed-to-expected ratio for ingredient inclusion using no commodity inclusion assistance technology or using commodity inclusion assistance technology; and 2) evaluate the influence that varying degrees (highly variable at random or 0.454 kg asfed tolerance for each ingredient) of accuracy on ingredient inclusion have on growth performance, carcass traits, efficiency of dietary NE utilization, and ingredient inventory management in finishing beef cattle. Experiment 1 used single source Black Angus heifers (n = 60; initial …
Effectiveness Of Atmospheric Cold Plasma In Inactivating Microorganisms, Nadee Shanika Kaluwahandi
Effectiveness Of Atmospheric Cold Plasma In Inactivating Microorganisms, Nadee Shanika Kaluwahandi
Electronic Theses and Dissertations
In the food processing industry, Cold Plasma (CP) is an emerging green process with a number of potential applications. Cold plasma has mostly been used to reduce microbial counts in foodstuffs and biological materials as well as in different levels of packaging, particularly in cases where there is thermal sensitivity. Recent studies have demonstrated that CP technology is being developed for use in the food and agriculture industries. The primary focus is on the interactions between reactive species and food-borne microbes to inactivate them. The literature review discusses both proven and potential applications for cold plasma in food processing, as …
Polysaccharide-Based Hydrogel Beads For Biological Nitrogen Fixation, Prashant Dahal
Polysaccharide-Based Hydrogel Beads For Biological Nitrogen Fixation, Prashant Dahal
Electronic Theses and Dissertations
No abstract provided.
Rye Cover Crop Termination Timing Influenced Early Corn Growth, Gene Expressions, And Yield In A No-Till System, Janet Moriles-Miller
Rye Cover Crop Termination Timing Influenced Early Corn Growth, Gene Expressions, And Yield In A No-Till System, Janet Moriles-Miller
Electronic Theses and Dissertations
No abstract provided.
Age‑Related Differences In Lower Limb Muscle Activation Patterns And Balance Control Strategies While Walking Over A Compliant Surface, Woohyoung Jeon, Ahmed Ramadan, Jill Whitall, Nesreen Alissa, Kelly Westlake
Age‑Related Differences In Lower Limb Muscle Activation Patterns And Balance Control Strategies While Walking Over A Compliant Surface, Woohyoung Jeon, Ahmed Ramadan, Jill Whitall, Nesreen Alissa, Kelly Westlake
Health & Kinesiology Faculty Publications and Presentations
Substantial evidence demonstrates that falls in older adults are leading causes of fatal and nonfatal injuries and lead to negative impacts on the quality of life in the aging population. Most falls in older fallers result from unrecoverable limb collapse during falling momentum control in the single limb support (SLS) phase. To understand why older adults are more likely to fall than younger adults, we investigated age-related differences in knee extensor eccentric control, lower limb muscle activation patterns, and their relation to balance control. Ten older and ten younger healthy adults were compared during balance control while walking on a …
Resistant Potato Starch Supplementation Reduces Serum Histamine Levels In Healthy Adults With Links To Attenuated Intestinal Permeability, Jason R. Bush, Jun Han, Edward C. Deehan, Scott V. Harding, Madhura Maiya, Joshua Baisley, David Schibli, David R. Goodlett
Resistant Potato Starch Supplementation Reduces Serum Histamine Levels In Healthy Adults With Links To Attenuated Intestinal Permeability, Jason R. Bush, Jun Han, Edward C. Deehan, Scott V. Harding, Madhura Maiya, Joshua Baisley, David Schibli, David R. Goodlett
Health & Kinesiology Faculty Publications and Presentations
Histamine from our diet or gut microbes can trigger gastrointestinal disturbances, and resistant potato starch (RPS) has previously been shown to alleviate these symptoms while increasing levels of health-associated bacteria such as Akkermansia through unknown mechanisms. Post hoc exploratory metabolomic analysis of serum amino acid, amine, and carnitine metabolites in participants consuming 3.5 g/day RPS or placebo (n = 48) was performed using liquid chromatography-mass spectrometry to determine whether RPS positively influences histamine metabolism and related parameters. Histamine levels were significantly reduced by RPS treatment, but histamine-degrading enzyme products were unaffected by RPS. RPS also reduced histamine-secreting Haemophilus and Lactobacillus. …
Dataset For Effects Of Single-Session Practice Structure On Motor Skill Acquisition And Alpha And Beta Eeg Oscillations, Audrey Porter, Ronald V. Croce, Wayne Smith
Dataset For Effects Of Single-Session Practice Structure On Motor Skill Acquisition And Alpha And Beta Eeg Oscillations, Audrey Porter, Ronald V. Croce, Wayne Smith
Faculty Publications
Although it is known that practicing a motor skill updates the associated internal model, it is still unclear as to how cortical oscillations linked with the motor skill change under differing practice schedules. The current study investigated α- and β-power changes associated with motor skill acquisition. Firstly, we investigated the behavioral effects of practice on motor learning and retention during repetitive (RP) and variable (VP) practice schedules on an anticipation timing task. Secondly, we investigated changes in cortical α (10-13 HZ) and β (15-30 Hz) event-related synchronization and dyssynchronization (ERS/ERD) under RP and VP during early (EP) and late …