Life Sciences Commons^™

Computed Tomography Image Analysis: Modeling Skeletal Injuries In Thoroughbred Racehorses, Grace Louise Camp

Theses and Dissertations--Veterinary Science

Musculoskeletal injuries account for the majority of career-ending health concerns in Thoroughbred racehorses. A small but very serious subset of these can be life-threatening and usually involve fractures of the bones in the lower limb that occur during high-speed exercise or racing competitions. Understanding the etiopathogenesis of lower forelimb bone fractures leading to catastrophic musculoskeletal injuries (CMI) is the highest safety and welfare priority in horse racing. This thesis conducted two studies utilizing computed tomography (CT) images of Thoroughbred lower limbs. The first examined bone lesion annotations in 134 horses (268 forelimbs) across 105 anatomical sites to assess the concordance …

Use Of Molecular Genetics To Investigate Population Structure And Swayback In Horses, Navid Yousefimashouf

Theses and Dissertations--Veterinary Science

The present research incorporated molecular genetic methods to 1) investigate the genetic basis of Juvenile Onset Lordosis or Swayback in the American Saddlebred horses; and 2) conduct a population genetic study to compare the Persian Kurdish, Persian Arabian and American Thoroughbred horse populations.

Juvenile-onset lordosis, or swayback, is a condition in horses where the conformational topline back curvature drops significantly within the first two years of life. The trait has a higher prevalence in Saddlebreds (5%). Prior research on them quantified the trait using a Measurement of Back Contour (MBC), defining an MBC of >7.0 centimeters as swayback, and8.0) MBC …

Receptor Usage And Infection Biology Of Influenza D Virus, Tirth Uprety

Theses and Dissertations--Veterinary Science

Influenza D virus (IDV) utilizes bovines as a primary reservoir with periodical spillover to other hosts. We have previously demonstrated by the traditional hemagglutination assay coupled with sialoglycan microarray and functional assays that IDV can recognize and bind both 9-O-acetylated N-acetylneuraminic acid (Neu5,9Ac₂) and 9-O-acetylated N-glycolylneuraminic acid (Neu5Gc9Ac). Agricultural animals such as cattle can produce both Neu5,9Ac₂ and Neu5Gc9Ac, while humans are genetically unable to synthesize Neu5Gc9Ac. The bifunctional enzyme, glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE) is a master regulator of de-novo sialic acid synthesis while 9-O-Acetylation of sialic acids is catalyzed by …

The Long-Term Effects Of Wildfire Severity On Oak-Pine Communities And Their Microclimates, Scott Glenn Culbert

Theses and Dissertations--Forestry and Natural Resources

In the eastern U.S., fire-dependent tree species have historically dominated upland forest communities, but are now experiencing widespread regeneration challenges as a result of 20^th century fire suppression policies, and are being replaced by mesophytic species. Wildfires that contain areas of high burn severity may provide an important means of mitigating these challenges and facilitating fire-dependent species regeneration and recruitment into larger size classes. One mechanism by which high-severity fire can accomplish this is by modifying understory microclimate characteristics to be more conducive to these species’ growth. A wildfire within the Daniel Boone National Forest, Kentucky, USA, provided the …

Economics Of Maple Syrup Production In Kentucky, Bobby Thapa

Theses and Dissertations--Forestry and Natural Resources

Maple syrup production is traditionally associated with New England regions in the United States, but there is growing interest in expanding it to other regions with suitable environmental conditions, including Kentucky. This study presents an in-depth analysis of the potential production and economic impacts of maple syrup in Kentucky using a multi-method approach. First, the study applies a stochastic production model to assess the effects of climatic and tree variables on maple syrup yield. The results reveal that several variables, including the number of maple trees, taps, temperatures, tapping season length, and time, significantly affect maple syrup yield. Second, input-output …

Effects Of Forest Reclamation And Landscape Features On Avian Occupancy, Species Richness, And Abundance In Appalachia, Rebecca N. Davenport

Theses and Dissertations--Forestry and Natural Resources

The Forestry Reclamation Approach (FRA) is a recently developed coal mining reclamation method that emphasizes best management practices in forestry, such as the planting of native trees and shrubs. Although the FRA is expected to benefit wildlife, no studies have empirically examined the effects of the FRA on avian species. My study aimed to identify which reclamation approaches and/or landscape features promote breeding songbirds, particularly mature forest avian guilds and species of conservation need. I conducted point count surveys in the Appalachian Mountains of eastern West Virginia and assessed differences in avian occupancy, species richness, and species abundance between four …

The Effect Of Land-Cover/Land-Use And Habitat Fragmentation On Spatial Patterns Of Lyme Disease In The Eastern United States, Maria Elizabeth Topmiller

Theses and Dissertations--Forestry and Natural Resources

Lyme disease has been of national concern for the past few decades. As our understanding of the role of landscape structure in epidemiology expands, it is essential to apply the principles of landscape ecology to the research of vector-borne and zoonotic disease. This study examined the strength of the relationship between land-cover class, degree of habitat fragmentation and county-level Lyme disease cases. Forest, agricultural, and urban land cover types were the categories of interest, while percent cover, edge density, and patch density were the landscape metric used to measure habitat amount and fragmentation. The general linear trends were modelled with …

Pond-Breeding Amphibian Responses To Wetland Creation And Reforestation On A Legacy Surface Mine In The Monongahela National Forest, Lauren Breanna Sherman

Theses and Dissertations--Forestry and Natural Resources

Surface mine reclamation has been an evolving practice since the Surface Mining Control and Reclamation Act was passed in 1977, holding mining companies accountable for returning ecological function to areas directly impacted by mining activities. One recent method of reclamation, the Forestry Reclamation Approach (FRA), aims to enhance reforestation and ecosystem function through the creation of wetlands, as opposed to traditional methods that often revert land to grasslands. However, wildlife response to FRA has rarely been investigated. The goal of this project was to analyze the effects of the four treatment types, FRA in two chronosequences, natural regeneration, and unmined …

Chemical Immobilization Of Helicopter-Captured Elk (Cervus Canadensis) And Survival Of Elk Calves In Southeastern Kentucky, Kathleen E. Williams

Theses and Dissertations--Forestry and Natural Resources

Safe and effective chemical immobilization is critical to minimize stress and risk of injury when capturing free-ranging, wild ungulates. Many traditionally favored high potency opioids have been phased out or become unavailable because of increased regulations, leading to the development of two pre-mixed combination drugs, butorphanol-azaperone-medetomidine (BAM) and nalbuphine-medetomidine-azaperone (NalMed-A). Both drugs have been used to chemically immobilize ungulates, but their efficacy has not been documented in elk captured and transported via helicopter. During 2020 – 2022, we chemically immobilized helicopter-captured female elk (Cervus canadensis) with a single IM-injection of BAM (n = 41) or NalMed-A (n = …

Applying Hermetic Storage And Multiple Abiotic-Stressed Germinations To Actuate Desirable Effects In Wheat Phytochemistry, Food, And Biological Functionalities, Bababode Kehinde

Theses and Dissertations--Biosystems and Agricultural Engineering

Wheat is one of the most widely consumed staple foods in the world, supplying both nutritional and caloric requirements for billions of people. The rising global population and increased food demand behooves all stakeholders across the wheat supply chain to significantly increase their output and efficiency. Beyond the target for increased farm yields, dietary, nutritional, and health requirements along with their continuous supply must be satisfied with improved storage and processing schemes. Post-harvest changes in wheat can result in a significant reduction in its quality. Such losses could be qualitative and/or quantitative, impacting its physical, physiological, organoleptic, and nutritional attributes. …

Improving Indoor Arenas For The Equine Industry, Staci Mcgill

Theses and Dissertations--Biosystems and Agricultural Engineering

Equine indoor arenas are a unique infrastructure investment found at equine farms and facilities. They are semi-indoor structures for exercising horses, exhibiting skills during competitive events, and other equine related activities. These spaces do not always include mechanical ventilation or stirring fans and occupancy by horses and humans can be sporadic and inconsistent, which creates a challenging space for understanding and predicting airflow. Typically, indoor arenas have a sand-based footing over which the horse travels. The impact of the hooves can cause dust to become a concern within the facilities. Environmental concerns within these facilities (temperature, respirable dust, moisture, and …

Managing Death In Termites, Jizhe Shi

Theses and Dissertations--Entomology

Death of individuals from the same species represents potential risks from disease, predation or competition for animals. Diverse responses associated with death have evolved ranging from simply avoiding or being attracted to the corpses in solitary animals to complicated undertaking behavioral repertoire in eusocial insects. A systematic review in chapter 1 suggested cannibalism is an ancestral and widespread death-related behavior in all non-human animals. Termites are suggested to switch their undertaking behavioral responses from cannibalism to burial based on interactions between chemicals associated with death to balance risks and benefits associated with decomposition. In eusocial animals like termites with caste …

Surveillance For Ticks And Tick-Borne Pathogens In Kentucky, Anna Rosalee Pasternak

Theses and Dissertations--Entomology

Tick-borne diseases are an emerging threat to human and animal health. In Kentucky, tick-borne disease surveillance has identified rising incidences of spotted fever rickettsiosis, ehrlichiosis, and Lyme disease. Since these diseases occur through the bites of infected ticks, effective prevention efforts are reliant upon knowing where the risk of exposure to tick bites exists. Historical data on tick distribution in Kentucky is variable, with very little reported on a statewide scale, leaving vector control workers, public health personnel, physicians, veterinarians, and others to rely on outdated, intermittent, or out-of-state information. In my dissertation, I surveyed ticks and select tick-borne pathogens …

Landscape Influences And Preventive Management For Drosophila Suzukii In Small Fruit Crops, Ryan William Kuesel

Theses and Dissertations--Entomology

Small fruit production has been greatly challenged by the repeated invasion of non-native arthropod pests, impacting economic feasibility, and increasing the need for insecticides. Spotted wing drosophila (Drosophila suzukii (Matsumura)) is the most recent invasive pest of small fruits. It causes damage by ovipositing beneath the skin of ripening fruits. Its wide-spread distribution and development of resistance to insecticides has resulted in the need for improved alternative management strategies for small fruit growers.

My surveys of D. suzukii at thirteen blackberry (Rubus fruticosus L.) and raspberry (Rubus idaeus L.) fields found that while D. suzukii was present …

Ecological Risk Assessment Of Transgenic Conditional Lethality Systems For Genetic Biocontrol Strategies, Fernan Rodrigo Perez-Galvez

Theses and Dissertations--Entomology

Genetic strategies of insect pest control are receiving increased interest due to recent advances in genetic engineering. By introducing alleles that make males functionally sterile, genetically modified (GM) insects can be used in area-wide pest management programs similar to the Sterile Insect Technique (SIT). Genetic control strategies carry several potential benefits, including improved efficacy and specificity, lower operation costs, and reduced dependence on chemical insecticides, but their use is still controversial despite extensive technical documentation indicating their innocuity to humans and the environment. This hesitation is likely due to the novel nature of the approach.

While the first field applications …

Alternative Pharmacology: Exploring Ketamine Use For Treatment Resistant Mental Health Disorders, Kiersten L. Ash

Soaring: A Journal of Undergraduate Research

No abstract provided.

Biomarker Identification For Breast Cancer Types Using Feature Selection And Explainable Ai Methods, David E. La Rosa Giraud

Honors Undergraduate Theses

This paper investigates the impact the LASSO, mRMR, SHAP, and Reinforcement Feature Selection techniques on random forest models for the breast cancer subtypes markers ER, HER2, PR, and TN as well as identifying a small subset of biomarkers that could potentially cause the disease and explain them using explainable AI techniques. This is important because in areas such as healthcare understanding why the model makes a specific decision is important it is a diagnostic of an individual which requires reliable AI. Another contribution is using feature selection methods to identify a small subset of biomarkers capable of predicting if a …

Covid-19 Vaccination And Alcohol Consumption: Justification Of Risks, Pavel A. Solopov

Bioelectrics Publications

Since the beginning of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic, pharmaceutical companies and research institutions have been actively working to develop vaccines, and the mass roll-out of vaccinations against COVID-19 began in January 2021. At the same time, during lockdowns, the consumption of alcoholic beverages increased. During the peak of vaccination, consumption remained at high levels around the world, despite the gradual relaxation of quarantine restrictions. Two of the popular queries on search engines were whether it is safe to drink alcohol after vaccination and whether this will affect the effectiveness of vaccines. Over the past two …

Assessment Of The Triple Reuptake Inhibitor Diclofensine: Effort-Based Decision-Making In A Rodent Model Of Motivational Dysfunction, Sofia Papanikolaou

Holster Scholar Projects

Serotonin-selective reuptake inhibitors (SSRIs) are the most commonly prescribed antidepressant medications. Despite their popularity, they remain relatively ineffective at treating effort-related motivational symptoms of depression such as fatigue and anergia. Increasing research on triple reuptake inhibitors (TRIs) that target three neurotransmitters—dopamine, serotonin, and norepinephrine—has suggested that TRIs could have efficacy in targeting motivational dysfunction due to their dopaminergic effects. Previous research has shown that the dopamine depleting agent tetrabenazine can reliably induce motivational deficits in rats, as evidenced by a shift towards low-effort behavior in effort-based choice tasks, and provide a validated approach to creating a model of motivational dysfunction. …

Creating An In-Vitro Co-Culture Model Of Human Megakaryocytic-Erythroid Progenitors And Endothelial Cells, Clerin John

Holster Scholar Projects

Previous studies have suggested that endothelial cells promote red blood cell (RBC) production in human bone marrow, which has implications for improved treatments for anemia, cancer, and reproduction. However, investigation of this phenomenon is hindered by a dearth of studies that have successfully isolated primary endothelial cells from human bone marrow. Using flow cytometry, magnetic cell separation, and primary cell culture techniques we tested the hypothesis that adult bone marrow-derived endothelial cells can be isolated from previously frozen bone marrow samples by selecting for CD144+ expressing cells and culturing them in vitro. Magnetic cell separation utilizing the marker CD144 to …

Effect Of Chitosan On Plant Growth, Flowering, And Substrate Characteristics Of Potted Geranium (Pelargonium X Hortorum), Maggie Liu

Holster Scholar Projects

Modern industrial agricultural practices rely heavily on the use of fertilizers and pesticides to increase crop quality and quality which overtime gradually degrades the quality of cultivated soils and aquatic environments. The purpose of this project is to determine how chitosan polymers with various molecular weights affect the growth and flowering of Geranium plants as well as the water and nutrient retention capacity of the substrate. Three chitosans with different molecular weights (2 kDa, 50 kDa, and 970 Da) were experimented on the geraniums. Each chitosan treatment was applied through a spray system onto the experimental group plants after transplantation …

Enhancing Tomato Fruit Quality: Application Of Crispr/Dcas9-Mediated Methylation On The Pg Gene Promoter Region, Irena Komninakas

Holster Scholar Projects

Commercially produced tomatoes are typically harvested well before maturation in order to withstand domestic and international distribution. While this process ensures unspoiled goods, the nutritional value of the fruits have been curtailed as a result of premature harvesting. Previous research has found that the polygalacturonase gene (PG gene) is responsible for cell wall degradation of the tomato. In this study, CRISPR/dCas9 technology was employed to increase methylation levels in the promoter regions of the PG gene. This method is significant in that it epigenetically modifies the tomato genome using a technique that has the potential to develop non-genetically modified progeny …

New Species Of Birds Reported In Arkansas Since 2009, R. Tumlison, R. Kannan

Journal of the Arkansas Academy of Science

We update our state of knowledge regarding the 20 new species of birds (and 1 new species name based on a change in taxonomy) discovered in Arkansas since the last update in 2009. These are based on records of the Arkansas Audubon Society and those posted on eBird. New species include 3 from Order Anseriformes, 1 Pelicaniformes, 1 Falconiformes, 1 Gruiformes, 4 Charadriiformes, 1 Apodiformes, and 10 Passeriformes. Historic distributions of the new species include Atlantic, Pacific, and Gulf Coasts, as well as interior origins. The current count of extant species of birds observed in Arkansas now stands at 425.

Rare Birds In Arkansas: Historical Observations And New Records, R. Tumlison, R. Kannan

Journal of the Arkansas Academy of Science

Diligent members of birding communities are quick to report rare sightings of birds, which often lead to multiple observations and photographic documentation. Verified reports and images of birds in Arkansas are curated by the Arkansas Audubon Society (AAS), which has led to an appreciation of which species are common versus rare. We gathered historic (literature) and more recent unpublished records of rare birds (those with fewer than 10 reports, per AAS) to document the historic and current state of knowledge of those species. Currently, there are a total of 425 species of birds reported in Arkansas, of which 54 are …

Least Flycatchers Under-Reported In Fall Migration In Arkansas —A Citizen Science Conundrum, L. Barnes, R. Kannan

Journal of the Arkansas Academy of Science

The Least Flycatcher Empidonax minimus is a small passerine migrant bird that passes through Arkansas in fall and spring migration. The birds are vocal during spring passage but relatively quiet and unobtrusive during fall migration, leading to many fewer reports by birdwatchers in fall than spring. We use eBird maps and data to present evidence to support this discrepancy between fall and spring observations. We also show that the species is under-reported in Arkansas during the fall compared to neighboring regions. This may be in part due to the reluctance of some birders to provide the minimal evidence required to …

Citizen Science: Role Of Inaturalist In Biodiversity Documentation And Education In Arkansas, K. Baker, L. Barnes, S. Rana, J. Jackson, R. Kannan

Journal of the Arkansas Academy of Science

iNaturalist is a global online digital platform for documenting and fostering interest in biodiversity, with Research Grade (RG) entries being vetted and scientifically valid observations. Here we present a review of the role of approximately 16,000 Arkansans using the iNaturalist tool for recording 445,000 verifiable observations comprising 10,800 species, with 40 percent RG, and one third of observations still pending confirmation. Overall, plants lead in RG observations followed by invertebrates and birds. Within these groups, reptiles lead in proportion of RG observations, followed by birds and amphibians. Less charismatic or infrequently encountered organisms are less represented. Arkansas ranks about average …

Energy Content Of Grasshoppers (Orthoptera: Acrididae) In The Diet Of Scaled Quail (Callipepla Squamata) In Southeastern New Mexico, J.L. Hunt, M.E. Grilliot, T.L. Best, C.M. Wilkerson, A.F. Huitt

Journal of the Arkansas Academy of Science

Grasshoppers (Orthoptera: Acrididae) are common insects that are important food items for many kinds of wildlife, including many species of upland game birds such as scaled quail (Callipepla squamata). We analyzed the energy content of grasshoppers obtained from the crops of scaled quail collected from plainsmesa sand-scrub in Lea and Eddy counties, New Mexico. Grasshoppers were removed from crops and dried for 48 hours at 60°C to remove moisture and standardize masses. Grasshoppers were then pulverized and analyzed for gross caloric value (i.e., energy content) in an oxygen bomb calorimeter. Energy content of grasshoppers from New Mexico averaged 19.2 J/kg …

Determining The Effects Of Water Quality On Stream Metabolic Function Of Urban Stream Corridors In Columbus, Georgia, Md Abu Raihan

Theses and Dissertations

Urbanization alters streams, disrupting their natural habitat and inducing changes in nutrient dynamics and primary productivity. These human-induced interventions diminish ecological structure, homogenizing once-diverse ecosystems and posing a risk of impairment or destruction. Continuous monitoring of stream ecosystem metabolism emerges as an essential tool for a comprehensive understanding and effective intervention in response to these challenges. This study delves into the intricate dynamics of nutrient levels and productivity within the urbanized context of the Weracoba Creek watershed located in Columbus, Georgia, USA. Through a cross-sectional study, we explore spatial and temporal variations in nutrient concentrations (NH4, NO3, PO4, TP, TN) …

Characterizing Genetic Diversity And Testing For Fisheries Induced Evolution In Southeastern Kansas Populations Of Bluegill (Lepomis Macrochirus), Morganne C. Borsh

Dissertations and Theses @ UNI

Bluegill (Lepomis macrochirus) is a popular and versatile sport fish with wide distribution. Fisheries management of the species is generally nebulous due to the additive role of environmental factors, genetics, social interactions, and alternative reproductive tactics in determining bluegill growth. Size structure could be further influenced by angling pressure: large parental males are more susceptible to harvest than smaller cuckolders. The systematic removal of large individuals through angling can result in stunted populations via fisheries induced evolution (FIE). In southeastern Kansas, bluegill from popularly fished impoundments are projected to have smaller sizes compared to isolated or inaccessible impoundments from the …

Assessment Of Conservation Reserve Program Enhancements At Contract Renewal: Impact On Monarch Butterfly (Danaus Plexippus) Habitat, Tristan L. Murphy

Dissertations and Theses @ UNI

The monarch butterfly (Danaus plexippus) is a migratory species whose primary breeding range is the upper Midwestern United States. In 2022, the International Union for the Conservation of Nature listed the monarch as endangered due to milkweed and migratory habitat loss. One of the most promising opportunities for monarch recovery is to enhance habitat quality on private land currently enrolled in the USDA Conservation Reserve Program (CRP). In order to qualify for re-enrollment in the program and continuation of annual government payments, farmers must show adequate habitat quality or enhance their site by overseeding after burning, herbicide application, …