Life Sciences Commons^™

Phase 1a Clinical Study For Q-Griffithsin Intranasal Spray For Prevention Of Coronavirus, Megan Bezold, Kenneth Palmer, Nobuyuki Matoba, Elizabeth Cash

Posters-at-the-Capitol

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a highly transmissible virus that causes acute respiratory disease with possible long-term complications, known as coronavirus disease 2019 (COVID-19). Griffithsin (GRFT; including the oxidation-resistant variant Q-GRFT used in this study) is an algal carbohydrate-binding protein exhibiting antiviral effects against many enveloped viruses, including all major variants of SARS- CoV-2 reported to date. Q-GRFT has been recombinantly manufactured in Nicotiana benthamiana plants, thoroughly purified, and formulated as an intranasal spray designed to be developed as a non-vaccine broad-spectrum prophylactic product for acute use. This is the first-in-human, randomized, double-blind, placebo-controlled study. The …

Minute Pirate Bug And Big-Eyed Bug Populations In Pyramided Bt Sweet Corn Bordered By Native Perennial And Pasture Border Rows, Hanna Waller, John D. Sedlacek, Karen L. Friley, Jenny Galvin, Anjana Duwal

Posters-at-the-Capitol

Sweet corn is an important vegetable crop grown in Kentucky. The major sweet corn ear pests are European corn borer, Ostrinia nubilalis, and corn earworm, Helicoverpa zea. Ecological control measures have been introduced to manage these pests, reducing use of broad spectrum insecticides. Conservation biological control (CBC) is a component of these approaches. CBC manages pests in agroecosystems using habitat management to enhance populations of natural enemies that are already present in the system. Another important advancement is the cultivation of Bacillus thuringiensis (Bt) sweet corn resistant to the Lepidopteran corn ear pests. This study used CBC of corn ear …

A Dynamical System Model Of Dengue Transmission For Rio De Janeiro, Brazil, Gregory Schmidt, Benjamin Whipple, Vinodh Chellamuthu, Xiaoxia Xie

Spora: A Journal of Biomathematics

The dengue virus is a serious concern in many parts of the world, including Brazil. As data indicates, a prominent vector for dengue is the mosquito Aedes aegypti. By using the dengue incidence records from the Brazilian SINAN database, we estimate the population of A. aegypti within the city of Rio de Janeiro. Using historical climate data for Rio de Janeiro and the computed population estimates, we extend an existing model for the population dynamics of mosquitoes to incorporate precipitation in aquatic stages of development for A. aegypti.

Subgenera Of Dacne Latreille, 1797 (Erotylidae: Erotylinae: Dacnini) Given Full Generic Rank, Paul E. Skelley

Insecta Mundi

Previously described subgenera of Dacne Latreille, 1797 (Coleoptera: Erotylidae) show charac­ter states and combinations implying closer relationships with genera other than with Dacne, suggesting “Dacne” is not monophyletic. These characters are briefly discussed along with the genera sharing the charac­ters. To improve the current classification, the subgenera of Dacne are raised to generic status as: Afrodacne Delkeskamp, 1954, Ameridacne Skelley, 2009, and Xenodacne Boyle, 1956. A checklist of all species included in these genera is presented, updating generic combinations as needed.

Clustering Climate And Management Practices To Define Environmental Challenges Affecting Gastrointestinal Parasitism In Katahdin Sheep, Brian C. Arisman, Joan M. Burke, James L. M. Morgan, Ronald M. Lewis

Department of Animal Science: Faculty Publications

Gastrointestinal nematodes (GIN) negatively affect the performance and well-being of sheep. Due to anthelmintic resistance, GIN are difficult to control leading producers to choose breeds that can exhibit resistance to parasitism. An example is Katahdin sheep. Katahdins are raised in various climates and management systems in the United States. These environmental factors can be combined to form eco-management groupings or clusters. We hypothesized that GIN challenge varies predictably based on the characteristics of these environmental clusters. Forty Katahdin producers from across the United States were surveyed for management information, with body weights (BW), fecal egg counts (FEC), and FAMACHA scores …

Determining Puma Habitat Suitability In The Eastern Usa, Veronica Yovovich, Nathaniel Robinson, Hugh Robinson, Michael J. Manfredo, Shelby Perry, Jeremy T. Bruskotter, John Vucetich, Luis Aníbal Solórzano, Lydia A. Roe, Alison Lesure, Jamie Robertson, Tom Bulter, L. Mark Elbroch

Michigan Tech Publications, Part 1

Pumas (Puma concolor) were eliminated from most of the eastern USA a century ago. In the past couple of decades, their recovery in the West has increased puma dispersal into the Midwest, with some individuals even traveling to the East Coast. We combined published expert opinion data and a habitat suitability index in an analysis that identified 17 areas in the Upper Midwest, Ozarks, Appalachia, and New England that could potentially host puma populations in the future. Thirteen of these were larger than 10,000 km2 and so likely to ensure a puma population’s long-term genetic health. Further, we quantified patch …

Untangling The Complex Interactions Between Turtle Ants And Their Microbial Partners, Manuela O. Ramalho, Corrie S. Moreau

Biology Faculty Publications

Background: To understand the patterns of biodiversity it is important to consider symbiotic interactions as they can shape animal evolution. In several ant genera symbiotic interactions with microbial communities have been shown to have profound impacts for the host. For example, we know that for Camponotini the gut community can upgrade the host's diet and is shaped by development and colony interactions. However, what is true for one ant group may not be true for another. For the microbial communities that have been examined across ants we see variation in the diversity, host factors that structure these communities, and the …

Selective And Differential Feeding On Marine Prokaryotes By Mucous Mesh Feeders, Carey P. Sweeney, Kelly R. Sutherland, Anne Thompson

Biology Faculty Publications and Presentations

Microbial mortality impacts the structure of food webs, carbon flow, and the interactions that create dynamic patterns of abundance across gradients in space and time in diverse ecosystems. In the oceans, estimates of microbial mortality by viruses, protists, and small zooplankton do not account fully for observations of loss, suggesting the existence of underappreciated mortality sources. We examined how ubiquitous mucous mesh feeders (i.e. gelatinous zooplankton) could contribute to microbial mortality in the open ocean. We coupled capture of live animals by blue-water diving to sequence-based approaches to measure the enrichment and selectivity of feeding by two coexisting mucous grazer …

Comparison Of In-Vitro 3d Human Embryoids With Current Models For Gastrulation, Jin Park

McKelvey School of Engineering Graduate Student Theses & Dissertations

Gastrulation is an early morphogenetic process that is conserved across most metazoans and lays out the future body plan through the formation and shaping of the three germ layers: endoderm, mesoderm, and ectoderm. Despite its importance, not much is known about the events surrounding human gastrulation that occurs in utero due to ethical and technical limitations on studying human embryos. Therefore, many researchers have devised protocols for creating in vitro models of gastrulation using embryonic stem cells. Initially starting with mouse embryonic stem cells, the field of in vitro embryo models has advanced rapidly, with protocols using human embryonic stem …

Microenvironmental Ammonia Enhances T Cell Exhaustion In Colorectal Cancer, Hannah N Bell, Amanda K Huber, Rashi Singhal, Navyateja Korimerla, Ryan J Rebernick, Marwa O El-Derany, Nupur K Das, Samuel A Kerk, Sumeet Solanki, Jadyn G James, Donghwan Kim, Li Zhang, Brandon Chen, Rohit Mehra, Timothy L Frankel, Balázs Győrffy, Eric R Fearon, Marina Pasca Di Magliano, Frank J Gonzalez, Ruma Banerjee, Daniel R Wahl, Costas A Lyssiotis, Michael Green, Yatrik M Shah

Faculty, Staff and Student Publications

Effective therapies are lacking for patients with advanced colorectal cancer (CRC). The CRC tumor microenvironment has elevated metabolic waste products due to altered metabolism and proximity to the microbiota. The role of metabolite waste in tumor development, progression, and treatment resistance is unclear. We generated an autochthonous metastatic mouse model of CRC and used unbiased multi-omic analyses to reveal a robust accumulation of tumoral ammonia. The high ammonia levels induce T cell metabolic reprogramming, increase exhaustion, and decrease proliferation. CRC patients have increased serum ammonia, and the ammonia-related gene signature correlates with altered T cell response, adverse patient outcomes, and …

Silicone Gel Sheeting For Treating Keloid Scars, Fan Tian, Qingling Jiang, Junjie Chen, Zhenmi Liu

Faculty, Staff and Student Publications

BACKGROUND: Keloid scarring is one of the most common types of pathological scarring. Keloid scars that fail to heal can affect a person's physical and psychological function by causing pain, pruritus, contractures, and cosmetic disfigurement. Silicone gel sheeting (SGS) is made from medical-grade silicone reinforced with a silicone membrane backing and is one of the most commonly used treatments for keloid scars. However, there is no up-to-date systematic review assessing the effectiveness of SGS for keloid scars. A clear and rigorous review of current evidence is required to guide clinicians, healthcare managers and people with keloid scarring.

OBJECTIVES: To assess …

Revision Of The Melanocytic Pathology Assessment Tool And Hierarchy For Diagnosis Classification Schema For Melanocytic Lesions: A Consensus Statement, Raymond L Barnhill, David E Elder, Michael W Piepkorn, Stevan R Knezevich, Lisa M Reisch, Boris C Bastian, Marcus Bosenberg, Klaus J Busam, Richard Carr, Alistair Cochran, Martin G Cook, Lyn M Duncan, Rosalie Elenitsas, Arnaud De La Fouchardière, Pedram Gerami, Iva Johansson, Jennifer Ko, Gilles Landman, Alexander J Lazar, Lori Lowe, Daniela Massi, Jane Messina, Daniela Mihic-Probst, Douglas C Parker, Birgitta Schmidt, Christopher R Shea, Richard A Scolyer, Michael Tetzlaff, Xiaowei Xu, Iwei Yeh, Artur Zembowicz, Joann G Elmore

Faculty, Staff and Student Publications

Importance: A standardized pathology classification system for melanocytic lesions is needed to aid both pathologists and clinicians in cataloging currently existing diverse terminologies and in the diagnosis and treatment of patients. The Melanocytic Pathology Assessment Tool and Hierarchy for Diagnosis (MPATH-Dx) has been developed for this purpose.

Objective: To revise the MPATH-Dx version 1.0 classification tool, using feedback from dermatopathologists participating in the National Institutes of Health-funded Reducing Errors in Melanocytic Interpretations (REMI) Study and from members of the International Melanoma Pathology Study Group (IMPSG).

Evidence review: Practicing dermatopathologists recruited from 40 US states participated in the 2-year REMI study …

Radiation Therapy For Retroperitoneal Sarcomas: A Strass-Ful Situation, Ahsan S Farooqi, B Ashleigh Guadagnolo, Devarati Mitra, Andrew J Bishop

Faculty, Staff and Student Publications

Locoregional recurrence (LRR) is the predominant pattern of relapse and often the cause of death in patients with retroperitoneal sarcomas (RPS). As a result, reducing LRR is a critical objective for RPS patients. However, unlike soft tissue sarcomas (STS) of the superficial trunk and extremity where the benefits of radiation therapy (RT) are well-established, the role of RT in the retroperitoneum remains controversial. Historically, preoperative or postoperative RT, either alone or in combination with intraoperative radiation (IORT), was commonly justified for RPS based on extrapolation from the superficial trunk and extremity STS literature. However, long-awaited results were recently published from …

Subtype And Site Specific-Induced Metabolic Vulnerabilities In Prostate Cancer, Federica Mossa, Daniele Robesti, Ramachandran Sumankalai, Eva Corey, Mark Titus, Jianhua Zhang, Francesco Montorsi, Christopher P Vellano, Joseph R Marszalek, Daniel E Frigo, Christopher J Logothetis, Taranjit S Gujral, Eleonora Dondossola

Faculty, Staff and Student Publications

Aberrant metabolic functions play a crucial role in prostate cancer progression and lethality. Currently, limited knowledge is available on subtype-specific metabolic features and their implications for treatment. We therefore investigated the metabolic determinants of the two major subtypes of castration-resistant prostate cancer [androgen receptor-expressing prostate cancer (ARPC) and aggressive variant prostate cancer (AVPC)]. Transcriptomic analyses revealed enrichment of gene sets involved in oxidative phosphorylation (OXPHOS) in ARPC tumor samples compared with AVPC. Unbiased screening of metabolic signaling pathways in patient-derived xenograft models by proteomic analyses further supported an enrichment of OXPHOS in ARPC compared with AVPC, and a skewing toward …

Inhibiting Androgen Receptor Splice Variants With Cysteine-Selective Irreversible Covalent Inhibitors To Treat Prostate Cancer, Thirumagal Thiyagarajan, Suriyan Ponnusamy, Dong-Jin Hwang, Yali He, Sarah Asemota, Daniel L Johnson, Weidong Zhou, Abhinav K Jain, Emanuel F Petricoin, Zheng Yin, Lawrence M Pfeffer, Duane D Miller, Ramesh Narayanan

Faculty, Staff and Student Publications

Androgen receptor (AR) and its splice variants (AR-SVs) promote prostate cancer (PCa) growth by orchestrating transcriptional reprogramming. Mechanisms by which the low complexity and intrinsically disordered primary transactivation domain (AF-1) of AR and AR-SVs regulate transcriptional programming in PCa remains poorly defined. Using omics, live and fixed fluorescent microscopy of cells, and purified AF-1 and AR-V7 recombinant proteins we show here that AF-1 and the AR-V7 splice variant form molecular condensates by liquid-liquid phase separation (LLPS) that exhibit disorder characteristics such as rapid intracellular mobility, coactivator interaction, and euchromatin induction. The LLPS and other disorder characteristics were reversed by a …

Evaluation Of Proton Therapy Reirradiation For Patients With Recurrent Head And Neck Squamous Cell Carcinoma, Anna Lee, Robbie Woods, Amgad Mahfouz, Sarin Kitpanit, Olivia Cartano, Irini Youssef, Kevin Sine, Dennis Mah, Brian Neal, Kaveh Zakeri, Jung J Kang, Nadeem Riaz, Yao Yu, Sean M Mcbride, Linda D Chen, C Jillian Tsai, Daphna Y Gelblum, Robert H Press, Loren S Michel, Eric J Sherman, David Pfister, Lara A Dunn, Alan L Ho, James Fetten, Richard J Wong, Jay O Boyle, Bhuvanesh Singh, Jennifer R Cracchiolo, Ian Ganly, Marc A Cohen, Nancy Y Lee

Faculty, Staff and Student Publications

Importance: Use of proton therapy reirradiation (PT-ReRT) for head and neck cancer is increasing; however, reports are heterogenous and outcomes can be difficult to interpret.

Objective: To evaluate outcomes and toxic effects following PT-ReRT in a uniform and consecutive cohort of patients with head and neck squamous cell carcinoma.

Design, setting, and participants: This retrospective cohort study included patients with recurrent primary head and neck squamous cell carcinoma who were treated with PT-ReRT from January 1, 2013, to December 31, 2020, at a single institution. Patient, clinical, and treatment characteristics were obtained, and multidisciplinary review was performed to record and …

Key Performance Indicators Detection Based Data Mining, Fatma Abogabal, Shimaa Mohamed Ouf, Amira M. Idrees Ami

Future Computing and Informatics Journal

One of the most prosperous domains that Data mining accomplished a great progress is Food Security and safety. Some of Data mining techniques studies applied several machine learning algorithms to enhance and traceability of food supply chain safety procedures and some of them applying machine learning methodologies with several feature selection methods for detecting and predicting the most significant key performance indicators affect food safety. In this research we proposed an adaptive data mining model applying nine machine learning algorithms (Naive Bayes, Bayes Net Key -Nearest Neighbor (KNN), Multilayer Perceptron (MLP), Random Forest (RF), Support Vector Machine (SVM), J48, Hoeffding …

Archy And Mehitabel, Don Marquis

Zea E-Books Collection

Archy and Mehitabel are two inimitable characters — a philosophical cockroach who types out free verse correspondence by dive-bombing the keys and an insouciant feline dancer out to take life for all it is worth, ever the lady and “toujours gai.”

Created by Don Marquis and popularized in the New York Sun and New York Herald-Tribune 1916–1922, their best-loved exploits and musings are captured in this marvellous collection of 48 episodes, and illustrated with 29 cartoon drawings by George Herriman. Archy sees the universe at an entirely different angle, and humanity is measured against its miniature insect reflections. We meet …

Effects Of High-Pressure Processing On Cooked Color And Eating Qualities Of Dark-Cutting Beef, Cole Reesman, Gary Sullivan, Mary-Grace C. Danao, Gretchen G. Mafi, Morgan Pfeiffer, Ranjith Ramanathan

Department of Animal Science: Faculty Publications

High meat pH leads to an undercooked or an abnormal pink appearance in fully-cooked product. High-pressure processing (HPP) promotes protein denaturation. The objective was to evaluate the effects of HPP on cooked steak color and sensory attributes of dark-cutting beef. USDA Choice (mean pH = 5.5) and dark-cutting (mean pH = 6.3) strip loin sections were vacuum packaged and treated with 0 (no HPP), 300, 450, and 600 MPa of pressure for 90 s using chilled water. Steaks were randomly assigned to measure external cooked color, Warner- Bratzler shear force, and trained sensory panel. Applying 300 MPa of pressure improved …

Still A Private Universe? Community College Students’ Understanding Of Evolution, Meredith A. Dorner, Philip Sadler, Brian Alters

Education Faculty Articles and Research

Background

Measuring what students know and retain about evolution is essential to improving our understanding of how students learn evolution. The literature shows that college students appear to have a poor understanding of evolution, answering questions on various instruments correctly only about half of the time. There is little research regarding evolution understanding among community college students and so this study examines if those students who are enrolled in life science classes, who are assessed using questions based on grade eight standards, show a better understanding of evolutionary principles than younger students and if there are differences in knowledge based …

The Impact Of Adolescent Maternal Age On Neonatal Outcomes And Neurodevelopment, Sarah Hanna

Honors Scholar Theses

To explore the association between maternal age and obstetric and neonatal outcomes with a focus on adolescent women between the age of 18 to 21 years. Secondary Analysis of RO1 cohort study. Maternity departments of six hospitals in Connecticut - Hartford Hospital, UConn John Dempsey Hospital, Saint Francis Hospital, Manchester Memorial Hospital, William W. Backus Hospital, or other - with relation to The University of Connecticut or Connecticut Children’s Medical Center. The primary outcomes were maternal and neonatal outcomes measured in addition to neonatal neurodevelopment. Neonates born to adolescent mothers exhibited lower birth weight (p=0.004). Based on the Bayley Scales …

Variability Of Hybridization In Topminnows, David D. Duvernell

Biological Sciences Faculty Research & Creative Works

Pairs of species that exhibit broadly overlapping distributions, and multiple geographically isolated contact zones, provide opportunities to investigate the mechanisms of reproductive isolation. Such naturally replicated systems have demonstrated that hybridization rates can vary substantially among populations, raising important questions about the genetic basis of reproductive isolation. The topminnows, Fundulus notatus and F. olivaceus, are reciprocally monophyletic, and co-occur in drainages throughout much of the central and southern United States. Hybridization rates vary substantially among populations in isolated drainage systems. We employed genome-wide sampling to investigate geographic variation in hybridization, and to assess the possible importance of chromosome fusions …

Clutch Analysis And Design For The Wildcat Pulling Team Quarter Scale Tractor Drivetrain, Lauren Doyle

Lewis Honors College Thesis Collection

The University of Kentucky (UK) Wildcat Pulling Team competes in the annual ASABE International Quarter Scale Tractor Student Design Competition. In 2022 the team placed 3rd overall in the competition, missing 1st place by 23 points. The loss in points was due to the clutch subassembly having issues related to maneuverability, manufacturability, and serviceability. The goal of this project was to redesign the 2022 tractor’s drivetrain for the new 2023 competition tractor. Background research was conducted on three types of clutches: manual, electric, and centrifugal. The electric clutch and centrifugal clutch with manual override were tested to determine their viability …

A Comparison Of Microsatellites And Single Nucleotide Polymorphisms For The Assessment Of Population Structure In The Amblyomma Maculatum Koch, 1844 Species Complex, Henrey A. Deese

Electronic Theses and Dissertations

The Gulf Coast tick, Amblyomma maculatum Koch, 1844 is widespread throughout North and Central America and is the primary vector for the emerging pathogen Rickettsia parkeri. In the U.S., the population in the Southwest (A. maculatum morphotype III) is morphologically different from the population east of the Rocky Mountains (A. maculatum morphotype II). This research tests the hypothesis that A. maculatum morphotype II and morphotype III represent distinct species through the analysis and comparison of 6 microsatellite loci and suite of 135,221 Single nucleotide polymorphisms (SNPs) generated from ddRADseq. Population genetic analysis of both microsatellites and SNPs …

Nationwide Assessment Of Leadership Development For Graduate Students In The Agricultural Plant Sciences, Karen Ferreira Da Silva, Ella Burnham, Joe Louis, Douglas Golick, Sydney E. Everhart

Department of Plant Pathology: Faculty Publications

Leadership development is a universally important goal across the agricultural plant science disciplines. Although previous studies have identified a need for leadership skills, less is known about leadership skill development in graduate programs. To address this, we constructed a mixed-method study to identify the most significant graduate school leadership experiences of scientists in the agricultural plant science disciplines. The survey was deployed to 6,728 people in the U.S. and received 1,086 responses (16.1% response rate). The majority of respondents reported that they were from one of the major agricultural states and employed at one of the agricultural plant science related …

Laser Light As An Emerging Method For Sustainable Food Processing, Packaging, And Testing, Prasad Chavan, Rahul Yadav, Pallavi Sharma, Amit Jaiswal

Articles

In this review article, we systematically investigated the diverse applications of laser technology within the sphere of food processing, encompassing techniques such as laser ablation, microbial inactivation, state-of-the-art food packaging, and non-destructive testing. With a detailed exploration, we assess the utility of laser ablation for the removal of surface contaminants from foodstuffs, while also noting the potential financial and safety implications of its implementation on an industrial scale. Microbial inactivation by laser shows promise for reducing the microbial load on food surfaces, although concerns have been raised about potential damage to the physio characteristics of some fruits. Laser-based packaging techniques, …

Assessing The Unionid Assemblage Of The Robert C. Byrd Pool, Ohio River, Ethan Hunter Bellamy

Theses, Dissertations and Capstones

Unionid mussels are the most imperiled taxa in the United States and are vastly understudied. The entire Robert C. Byrd (RCB) pool of the Ohio River is the study area. I selected sites from the 2019 and 2013 ORSANCO RCB pool assessments that utilize random site selection across the pool. I used SCUBA to survey and collect data on unionid diversity, reproduction, and habitat. I collected 1,083 individuals over 19 species in RCB pool. I compared results from RCB pool survey to two similar surveys conducted in Greenup Pool. Both richness and abundance in the Upper Section of Greenup Pool …

A New Species Of Spodochlamys Burmeister, 1855 (Coleoptera: Scarabaeidae: Rutelinae: Anatistini) From Colombia And A Key And Checklist Of Spodochlamys Species, Kazuo Kobayashi, Brett Ratcliffe

University of Nebraska State Museum: Entomology Papers

Spodochlamys keitai Kobayashi and Ratcliffe is described as a new species from Otanche in Boyacá Department, Western Boyacá Province, in northeastern Colombia. A description, diagnosis separating it from similar species, and illustrations are provided. An identification key and a checklist of Spodochlamys Burmeister species are included.

Assessing Ecotoxicity Of Surface Water And Sediment In The Little Scioto River, Leah Suzanne Morgan

Theses, Dissertations and Capstones

Aquatic toxicology evaluates the impact of pollution on freshwater and marine biota. Polycyclic aromatic hydrocarbons (PAHs) are one class of chemicals prevalent in the environment that impair aquatic organisms. This study examined the effect of contaminated surface water and sediments from a former wood treatment facility on four aquatic species: Pimephales promelas (fathead minnow), Ceriodaphnia dubia (water flea), Chironomus dilutus (midge), and Daphnia magna (water flea). The findings showed that sediment contamination had no significant effect on survival and growth of C. dilutus or on survival of D. magna. Surface water contamination began to have a significant effect on …

Characterizing The Vegetation And Effects Of Climate Change On Parris Island, A Sea Island Ecosystem, Cody Hart Goodson

Theses, Dissertations and Capstones

Coastal habitats provide many ecosystem services, protecting coastlines from storm surges and erosion, diminishing the effects of eutrophication, sequestering large amounts of carbon, and acting as vital wildlife habitat. Sea-level rise and increased storm surge intensity associated with climate change are increasingly disrupting coastal habitats. These disturbances can shift environmental gradients that drive the zonation of coastal vegetation types, driving habitat conversion. Monitoring coastal habitat conversion can improve our understanding of the dynamic effects of climate change on these landscapes. Therefore, our objectives for chapter 1 were to identify and describe the distributions of vegetation types present on Marine Corps …