Life Sciences Commons^™

The Efficacy Of Genethresher® Methylation Filtering Technology In The Plant Kingdom, U. Warek, J. A. Bedell, M. A. Budiman, A. N. Nunberg, R. W. Citek, D. Robbins, N. Lakey, P. D. Rabinowicz

IGC Proceedings (1977-2023)

The genomes of many plants are known to be composed of a large fraction of repetitive DNA, while a small portion is dedicated to genes. The bulk of the repetitive DNA constitutes transposable elements and is heavily methylated. GeneThresher technology has been developed to take advantage of these differential methylation patterns by filtering genomic shotgun libraries to exclude methylated sequences (Rabinowicz et al., 1999; Palmer et al., 2003; Martienssen et al., 2004). The result is a gene-enriched genomic shotgun library. Random shotgun sequencing of plant gene space, enabled by GeneThresher technology, is a rapid and cost-effective strategy …

Translational Genomics For Alfalfa Varietal Improvement, G. D. May

IGC Proceedings (1977-2023)

Key points

1. Medicago truncatula is a model legume with available mapping, genome, and RNA, protein and metabolite profiling databases and genetically diverse populations.
2. Genomics resources developed for M. truncatula have application in the study and improvement of alfalfa making it an excellent model for this forage legume.

Species Richness Affects Grassland Yield And Yield Stability Across Seasons, Sites And Years, David J. Barker, Reuben M. Sulc, M. R. Burgess, T. L. Bultemeier

IGC Proceedings (1977-2023)

The benefits of biodiversity (specifically species richness) are proposed to include both greater yield and greater stability of yield in a variable environment (Sanderson et al., 2004). Experimental evidence showing yield benefits is inconsistent (White et al., 2004). There is relatively little experimental data showing the effects of species richness on yield stability. The objective of this study was to measure the yield from mixtures with up to 12 species, and to measure the variability of yield between 2 sites, between spring and summer, and in 2 successive years.

Discovery And Functional Categorisation Of Expressed Sequence Tags From Flowers Of Eragrostis Curvula Genotypes Showing Different Ploidy Levels And Reproductive Modes, V. Echenique, S. Felitti, N. Paniego, L. Martelotto, S. C. Pessino, D. Zanazzi, P. Fernández, M. Díaz, P. Polci, G. C. Spangenberg

IGC Proceedings (1977-2023)

Two novel genotypes of weeping lovegrass (Eragrostis curvula) - a dihaploid strain obtained in vitro from an apomictic cultivar and a tetraploid plant derived from the dihaploid after chromosome duplication – have recently been developed. These materials represent an excellent system for the identification, through transcriptional profiling, of genes involved in diplospory and/or ploidy level gene regulation. The aim of this work was the discovery and functional classification of expressed sequence tags (ESTs) from immature inflorescences of the apomictic E. curvula cultivar Tanganyika (2n=4x=40), a dihaploid sexual strain derived from it (2n=2x=20) and a tetraploid sexual strain …

Protection Of Agrobiodiversity: Model Calculations In Rhineland-Palatia: Costs And Implications For Farmers, H. Bergmann

IGC Proceedings (1977-2023)

Biological conservation and production use the same areas of land in less favoured areas. Grassland in these areas makes an important contribution to the protection of agro-biodiversity. However, under existing market conditions and production needs, the use of low yielding grasslands is not economically efficient. The objective of this study was to analyze the economic consequences of different mowing strategies in a small region in Rhineland-Palatia (Germany) that served the protection of two butterfly species.

Stocking Rate Theory And Profit Drivers In North Australian Rangeland Grazing Enterprises, Neil D. Macleod, A. J. Ash, John G. Mcivor

IGC Proceedings (1977-2023)

Setting correct stock numbers is a key decision for successful pastoralism. In marginal environments, typified by northern Australia, this involves careful cattle herd management across landscapes and seasons characterised by heterogeneous land condition and extreme climatic uncertainty. Stocking rate theory which links animal production to stocking rates concentrates only on liveweight gain of sale animals and ignores complex herd (e.g. reproduction, mortality) and pasture dynamics (e.g. land condition) and costs of maintaining stock numbers (e.g. supplementary feeding). Related economic models are generally naïve and incomplete, being based on liveweight gain, meat prices and variable husbandry costs (e.g. Workman, 1986). Modelling …

An Ecological And Economic Risk Avoidance Drought Management Decision Support System, R. K. Heitschmidt, L. T. Vermeire

IGC Proceedings (1977-2023)

Ecologists have long recognized the fundamental impacts of drought on rangeland structure and function. Simulation models have been developed to increase our understanding of these impacts as they relate to forage production, particularly for predictive purposes. Although the capacity of these models to accurately predict quantity and quality of forage produced under varying climatic conditions is often quite good, their ability to serve as an effective and proactive drought management decision support system is often limited. This is in large part because their complexity impedes their use by on-the-ground managers. The objective of this research was to develop a very …

Predicting The Effects Of Management On Upland Birds, Economy And Employment, S. M. Gardner, G. M. Buchanan, J. W. Pearce-Higgins, M. C. Grant, A. Waterhouse

IGC Proceedings (1977-2023)

Livestock farming systems play a significant role in the economy and conservation of the UK uplands and rely heavily upon public financial support. Changes in that support could have far-reaching impacts on the wildlife interest and socio-economics of upland areas. Predicting the impacts of such changes is difficult, since they arise from responses to new economic circumstances. The effect of management change is also influenced by natural variation, such as the mosaic of plant communities, already present in the upland landscape. This paper sets out an approach that integrates theoretical models with field studies to investigate the effects of management …

Managing Resources By Grazing In Grasslands Dominated By Dominant Shrub Species, D. Magda, Cyril Agreil, Michel Meuret, E. Chambon-Dubreuil, P. -L. Osty

IGC Proceedings (1977-2023)

The European natural grasslands are attracting new attention because of their environmental value as habitats for threatened fauna and flora species and their contribution to the diversity of landscapes. Those responsible for the implementation of the European agri-environmental policy are hence encouraging livestock farmers to adopt grazing practices that contribute to the conservation of grassland biodiversity especially by limiting encroachment by dominant shrubs. However, current scientific knowledge and technical information are often insufficient to connect flock feeding and the impact of grazing on shrub dynamics and livestock farmers are not very enthusiastic about restoring or conserving “plant mosaics” including shrubs …

Challenges In Modelling Live-Weight Change In Grazed Pastures In The Australian Subtropics, C. K. Mcdonald, A. J. Ash

IGC Proceedings (1977-2023)

In sub-tropical regions there is enormous seasonal, annual and spatial variation in pasture quality and considerable variation in quality between pasture species. The heterogeneous structure of sub-tropical pasture swards means that process based modelling of liveweight change (LWC) is particularly difficult. In response to this complexity LWC has been expressed as a function of the length of the growing season and/or pasture utilization (McKeon et al. 2000), green leaf availability, or pasture availability and climate (Hirata et al. 1993). However, these relationships vary from year to year, often fail when species composition changes, and generally explain

Vegetation Dynamics Of Campos Under Grazing/Fire Regimes In Southern Brazil, Fernando L. F. De Quadros, J. P. P. Trinidade, D. G. Bandinelli, L. Pötter

IGC Proceedings (1977-2023)

Natural grassland vegetation in Southern Brazil, known as campos, has most likely evolved under a disturbance regime that included fire and grazing (Pillar et al., 1997). Nowadays, the composition of the vegetation of campos is grazing- and fire-dependent (Boldrini et al., 1997). Its importance can be evaluated by the fact that it represents 37 % of the state’s area and provides 77 % of the slaughtered cattle at Rio Grande do Sul (Barcellos et al., 2002). The objective of this experiment was to evaluate the vegetation dynamics of campos under grazing/fire regimes in order to explore the …

Accumulation Of Polyphenols And Major Bioactive Compounds In Plantago Lanceolata L. As A Medicinal Plant For Animal Health And Production, Y. Tamura, K. Yamaki

IGC Proceedings (1977-2023)

Producing animals without the use of feed-grade antibiotic growth promoters and chemical medicines is sought. Scientific studies with this aim have focused on medicinal plants to identify and quantify any beneficial effects that they might have on animal production. Plantago lanceolata L. has been used in herbal medicines and is currently being evaluated as a potential pasture species because of its beneficial effects on animal health. In the present study, the accumulation of polyphenols in P. Lanceolata is compared to that in principal pasture species, and genetic variation and environmental changes in the major bioactive compounds in P. Lanceplata are …

Effect Of Urea-Treated Pennisetum Pedicellatum And Supplementation Of Concentrates With Urea On Milk Production Of “Mossi” Ewes, V. M. C. Bougouma-Yameogo, A. J. Nianogo

IGC Proceedings (1977-2023)

The “Mossi” sheep is a near parent of “Djallonke” sheep that live in sudano-sahelian area of Burkina Faso. However, there are few available results on dairy production from this breed. The treatment of straw with urea is a technique used in several developing countries to improve the nutritional value of gramineous forages (Sourabié et al., 1995). The aim of this study was to test the influence on the performance of “Mossi” ewes and on milk composition of treatment of Pennisetum pedicellatum (Pp) with urea in comparison with addition of urea to the concentrate feed.

Effect Of Stocking Rate On A Stipa Breviflora Desert Steppe Community Of Inner Mongolia, G. Han, Walter D. Willms, M. Zhao, A. Gao, S. Jiao, David R. Kemp

IGC Proceedings (1977-2023)

Stocking rate is an important factor in grazing management. The stocking rate defines utilization and ultimately grazing pressure, which in turn affects grassland sustainability. Grassland sustainability is partly defined by its species composition and ultimately by its productivity. These attributes are unique for specific plant communities and the effect of stocking rate must be established for each in order to understand the community response to grazing and to determine its carrying capacity. While some information exists on the effects of stocking rate on livestock production in the Stipa breviflora Griseb. Desert Steppe (Wei et al., 2000), the effects on …

Using The N-Alkane Technique To Estimate The Herbage Intake And Diet Composition Of Cattle Grazing A Miscanthus Sinensis Grassland, Y. Zhang, Y. Togamura, K. Otsuki

IGC Proceedings (1977-2023)

Plant wax alkanes are now widely used as marker substances (Dove & Mayes 1991) for the estimation of forage intake and diet composition of grazing herbivores. The objective of this study was to evaluate this method with cattle grazing a M. sinensis grassland in Japan.

Reasons For The Premature Decline In Astragalus Adsurgens Stands In Kerqin Sandy Land, Qizhong Sun, Z. L. Wang, J. G. Han, Y. W. Wang, G. R. Liu

IGC Proceedings (1977-2023)

Diseases partly account for reductions in Astragalus adsurgens stand longevity. The effect of some cultural practices on the control of pests and diseases have been reported (Hou, 1986; Nan, 1996), but few reports have detailed the relationship among soil fertiliser status, diseases and premature stand decline. This study was conducted to investigate these relationships in order to extend the longevity of Astragalus adsurgens stands.

Selecting Grassland Species For Saline Environments, M. E. Rogers, A. D. Craig, T. D. Colmer, R. Munns, S. J. Hughes, P. M. Evans, P. G. H. Nichols, R. Snowball, D. Henry, J. Deretic, B. Dear, M. Ewing

IGC Proceedings (1977-2023)

In Australia, around 5.7 million hectares of agricultural land are currently affected by dryland salinity or at risk from shallow water tables and this figure is expected to increase over the next 50 years (LWRA, 2001). Most improved grassland species cannot tolerate the combined effects of salt and waterlogging and, therefore, the productivity of sown grasslands in salt-affected areas is low. However, there is potential to overcome the lack of suitably adapted fodder species by introducing new, salt and waterlogging-tolerant species and by diversifying the gene pool of proven species. Potential species include exotic, naturalised and native Australian grass, legumes, …

Effects Of The Stage Of Growth And Inoculation On Proteolysis In Field Pea Silage, L. Cavallarin, G. Borreani, S. Antoniazzi, E. Tabacco

IGC Proceedings (1977-2023)

Ensiling legumes is a good way of providing home-grown protein in dairy farms but severe protein degradation can occur when conserving legumes. Peas (Pisum sativum L.) are legumes with a high crude protein and starch content, that provide a high forage yield in a short growing period. Very little information is available on the protein value of field pea silage. The aim of the study was to investigate the effect of stage of maturity and inoculant application on proteolysis in field pea silage in the Po Valley, NW Italy.

Grass Silage: Factors Affecting Efficiency Of N Utilisation In Milk Production, P. Huhtanen, K. J. Shingfield

IGC Proceedings (1977-2023)

Key points

1. Low efficiency of N utilisation for milk production in cows fed grass silage-based diets is mainly due to excessive N losses in the rumen.
2. The type and extent of in silo fermentation can alter the balance of absorbed nutrients.
3. There is very little experimental evidence that the capture of N in the rumen can be improved by a better synchrony between energy and N release in the rumen. Nitrogen losses in the rumen can be reduced by decreasing the ratio between rumen degradable N and fermentable energy.
4. Rapeseed meal has increased milk protein output more than isonitrogenous soybean …

Decision Support For Grassland Systems In Developing Countries, P. K. Thornton

IGC Proceedings (1977-2023)

Key points

1. Information flows in complex systems are often themselves highly complex, and decision support approaches based on linear input-output processes may have only limited impact.

2. How decisions are made, and how they can be appropriately supported, is often incompletely understood, in part because of inadequate understanding of the objectives and attitudes of all the decision makers involved.

3. Much of the developing world faces daunting problems in the coming 30 years, and appropriate information could play a critical role in dealing with these.

4. System complexity, household variability, and institutional intricacies have to be embraced rather than …

Polyvinylpyrrolidone-Passivated Fluorescent Iron Oxide Quantum Dots For Turn-Off Detection Of Tetracycline In Biological Fluids, Sri Sudewi, Lutfi Chabib, Muhammad Zulfajri, Gangaraju Gedda, Genin Gary Huang

Journal of Food and Drug Analysis

Tetracycline is an antibiotic that has been prescribed for COVID-19 treatment, raising concerns about antibiotic resistance after long-term use. This study reported fluorescent polyvinylpyrrolidone-passivated iron oxide quantum dots (IO QDs) for detecting tetracycline in biological fluids for the first time. The as-prepared IO QDs have an average size of 2.84 nm and exist a good stability under different conditions. The IO QDs’ tetracycline detection performance could be attributed to a combination of static quenching and inner filter effect. The IO QDs displayed high sensitivity and selectivity toward tetracycline and achieved a good linear relationship with the corresponding detection limit being …

Chemical Transformation Of Cannabidiol Into Psychotropic Cannabinoids Under Acidic Reaction Conditions: Identification Of Transformed Products By Gc-Ms, Minsun Jeong, Sangin Lee, Chaeyoung Seo, Eunjeong Kwon, Soohyang Rho, Mansoo Cho, Moon Yeon Kim, Wonwoong Lee, Yong Sup Lee, Jongki Hong

Journal of Food and Drug Analysis

Recently, cannabidiol (CBD), one of the major components of the Cannabis species, has been a focus in the cannabis industry due to its various pharmacological effects. Interestingly, CBD can be converted into several psychoactive cannabinoids, such as 9-tetrahydrocannabinol (Δ⁹-THC) and its structural isomers, under acidic reaction conditions. In this study, chemical transformation of CBD in ethanol solution was conducted with variation in pH at 2.0, 3.5, and 5.0 by addition of 0.1 M hydrochloric acid (HCl). These resulting solutions were derivatized with trimethylsilyl (TMS) reagent and analyzed using GC/MS-scan mode. Time profiles of CBD degradation and transformation of …

Development Of A Metabolomics-Based Data Analysis Approach For Identifying Drug Metabolites Based On High-Resolution Mass Spectrometry, Hsiao-Hsien Ting, Yi-Shiou Chiou, Tien-Yi Chang, Guan-Yu Lin, Pei-Jhen Li, Chia-Lung Shih

Journal of Food and Drug Analysis

A metabolomics-based approach to data analysis is required for drug metabolites to be identified quickly. This study developed such an approach based on high-resolution mass spectrometry. Our approach is a two-stage one that combines a time-course experiment with stable isotope tracing. Pioglitazone (PIO) was used to improve glycemic management for type 2 diabetes mellitus. Consequently, PIO was taken as a model drug for identifying metabolites. During Stage I of data analysis, 704 out of 26626 ions exhibited a positive relationship between ion abundance ratio and incubation time in a time-course experiment. During Stage II, 25 isotope pairs were identified among …

Untargeted Metabolomics Analysis Assisted By Signal Selection For Comprehensively Identifying Metabolites Of New Psychoactive Substances: 4-Meo-Α-Pvp As An Example, Hsin-Yi Wu, Yuan-Chih Chen, Jing-Fang Hsu, Hsiang-Ting Lu, Yu-Yi Pan, Mi-Chia Ma, Pao-Chi Liao

Journal of Food and Drug Analysis

New psychoactive substances (NPS) have been rapidly emerged as legal alternatives to controlled drugs, which raised severe public health issue. The detection and monitoring of its intake by complete metabolic profiling is an urgent and vital task. Untargeted metabolomics approach has been applied for several NPS metabolites studies. Although the number of such works is relatively limited but with a rapidly growing need. The present study aimed to propose a procedure that includes liquid chromatography high-resolution mass spectrometry (LC-HRMS) analysis and a signal selection software, MetaboFinder, programed as a web tool. The comprehensive metabolites profile of one kind of NPS, …

Guilu Erxian Jiao Enhances Protein Synthesis, Glucose Homeostasis, Mitochondrial Biogenesis And Slow-Twitch Fibers In The Skeletal Muscle, Wei-Yu Fang, Wan-Hsuan Chang, Yi-Hong Tsai, Hung-Te Hsu, Fang-Rong Chang, Chih-Lung Lin, Yi-Ching Lo

Journal of Food and Drug Analysis

Guilu Erxian Jiao (GEJ) is a commonly used nutritional supplement due to its rich content of amino acids. It is also a traditional herbal medicine for improving degenerative joint. This study aimed to investigate the effect and mechanism of GEJ water extract (GEJ-WE) on skeletal muscle in C2C12 myotubes and C57BL/6J mice. Analysis of GEJ-WE were performed by high-performance liquid chromatography fingerprinting with chemical standards. Protein expression, mRNA level, glycogen content, mitochondria activity and ATP level were evaluated by western blots, real-time PCR, PAS staining, MTT and ATP bioluminescence assay, respectively. Skeletal muscle strength was evaluated by grip strength. Skeletal …

High-Resolution 1h Nmr Analysis Of Continuous And Discontinuous Thermo-Oxidative Susceptibility Of Culinary Oils During Frying At 180℃, Gilbert Ampem, Adam Le Gresley, Martin Grootveld, Declan P Naughton

Journal of Food and Drug Analysis

Lipid oxidations products (LOPs) are reactive mutagenic and carcinogenic species known to be generated in thermally stressed culinary oils. Mapping the evolution of LOPs in culinary oils exposed to standard frying practices – both continuous and discontinuous thermo-oxidation – at 180℃ is vital to our understanding of these processes, and to the development of scientific solutions for their effective suppression. Modifications in the chemical compositions of the thermo-oxidised oils were analysed using a high-resolution proton nuclear magnetic resonance (¹H NMR) technique. Research findings acquired showed that polyunsaturated fatty acid (PUFA)-rich culinary oils were the most susceptible to thermo-oxidation. …

Investigation Of Monacolin K, Yellow Pigments, And Citrinin Production Capabilities Of Monascus Purpureus And Monascus Ruber (Monascus Pilosus), Tzu-Shing Lin, Shih-Hau Chiu, Chien-Chi Chen, Chih-Hui Lin

Journal of Food and Drug Analysis

Red mold rice (RMR) is a traditional Chinese medicine prepared using Monascus fermentation. M. ruber (pilosus) and M. purpureus have a long history of use as food and medicine. As an economically important starter culture, the relationship between the taxonomy of Monascus and production capabilities of secondary metabolites is crucial for the Monascus food industry. In this study, monacolin K, monascin, ankaflavin, and citrinin production by M. purpureus and M. ruber were genomically and chemically investigated. Our findings suggest that M. purpureus can produce monascin and ankaflavin in a correlated manner, whereas M. ruber produces monascin with minimum …

Determination Of 24 Sulfonamide Antibiotics In Instant Pastries By Modified Quechers Coupled With Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry, Shuo Li, Can Zhang, Hui-Xin Tang, Yue Gu, Ai-Jing Guo, Ke Wang, Kao-Qi Lian

Journal of Food and Drug Analysis

There were few reports about antibiotic residues in egg-containing products. In the study, an effective method for the simultaneous determination of 24 sulfonamide antibiotics in two instant pastries based on a modified QuEChERS sample preparation technique coupled with ultra performance liquid chromatography-tandem mass spectrometry was developed. The results show that the average recoveries of the SAs at 5, 10, and 50 µg·kg^-1 levels were 67.6%-103.8%, with relative standard deviations (RSD) of 0.80-9.23%. The limit of detections (LODs) and limit of quantitations (LOQs) were 0.01-0.14 µg∙kg^-1 and 0.02-0.45 µg∙kg^-1, respectively. This method was suitable for analysis of …

Simultaneous Determination Of 24 Congeners Of 2- And 3‑Monochloropropanediol Esters And 7 Congeners Of Glycidyl Esters Using Direct Multi-Residue Analytical Lc-Ms/Ms Methods In Various Food Matrices, Ching-Chang Lee, Bo-Lun Lin, Yi-Wen Huang, Ning-Syuan Hsu, Wei-Hsiang Chang

Journal of Food and Drug Analysis

Glycidyl esters (GEs) and 2- and 3-monochloropropanediol esters (MCPDEs) are emerging process-generated food contaminants known as possible carcinogens. Herein, a direct method is developed and validated for the first time to simultaneously quantify seven GEs and twenty-four MCPDE congeners of processed foods using liquid chromatography-tandem mass spectrometry in a single sequence without ester cleavage or derivatisation, thereby allowing for the simultaneous analysis of numerous food matrices with high accuracy and precision. Our results show levels of GEs varying from

Erinacine S From Hericium Erinaceus Mycelium Promotes Neuronal Regeneration By Inducing Neurosteroids Accumulation, Chun-Yu Lin, Yi-Ju Chen, Chih-Hsuan Hsu, Yu-Hsuan Lin, Peng-Tzu Chen, Ting-Han Kuo, Chris T. Ho, Hsin-Hua Chen, Sih-Jia Huang, Ho-Chieh Chiu, Chin-Chu Chen, Eric Hwang

Journal of Food and Drug Analysis

Erinacines derived from Hericium erinaceus have been shown to possess various health benefits including neuroprotective effect against neurodegenerative diseases, yet the underlying mechanism remains unknown. Here we found that erinacine S enhances neurite outgrowth in a cell autonomous fashion. It promotes post-injury axon regeneration of PNS neurons and enhances regeneration on inhibitory substrates of CNS neurons. Using RNA-seq and bioinformatic analyses, erinacine S was found to cause the accumulation of neurosteroids in neurons. ELISA and neurosteroidogenesis inhibitor assays were performed to validate this effect. This research uncovers a previously unknown effect of erinacine S on raising the level of neurosteroids.