Life Sciences Commons^™

Effects Of Sow Vocalization And Scent On Complete Blood Cell Count During Early Weaning Period, Mason Paladino

Biological Sciences Undergraduate Honors Theses

Piglets are deprived of their sows’ touch, sound, and smell after weaning which results in biological stress. The present study investigated continuing exposure of sow vocalization and scent during the early weaning period on complete blood cell count. A total of 12 litters, 8 piglets (4 male and 4 female) per litter were selected at weaning and assigned to 1 of 4 experimental treatments: control, O (olfactory group with scent and no vocal stimulation), V (non-olfactory with vocal stimulation), VO (vocal stimulation and olfactory stimulation) as a 2x2 factorial arrangement. Vocal stimulation was emulated by an audio recording of the …

Investigating The Role Of The Sumo-Activating Enzyme, Uba2, In Infection-Related Morphogenesis Of Magnaporthe Oryzae, Rachel Taylor

Biological Sciences Undergraduate Honors Theses

The plant pathogenic fungus Magnaporthe oryzae forms a specialized pressure-generating infection cell called an appressorium on the leaf surface, which it uses to mechanically rupture the otherwise impenetrable cuticle, enabling colonization of the underlying tissue and the establishment of blast disease. Appressorium differentiation by M. oryzae is cell cycle-regulated and requires programmed cell death of a three-celled propagative spore, as well as extensive remodeling of the microtubule, actin, and septin cytoskeletons. Recent studies have proposed the importance of sumoylation – the enzymatic conjugation of a small ubiquitin-like modifier to target proteins, for various aspects of infection-related development by M. oryzae, …

An Overview Of Serial Depletions Of Global Marine Fisheries 1950 To 2019, Alison Follmer

Biological Sciences Undergraduate Honors Theses

Overfishing is a global issue that poses a significant risk to the entire ocean ecosystem in diminishing biodiversity and ecosystem function. This thesis examined the pattern and pace of fisheries depletions due to commercial fishing during the past 70 years. The Food and Agriculture Organization of the United Nations (FAO) Division of Fisheries and Aquaculture maintains a database of global hauls of marine taxa (reported in metric tonnes) from 1950 – 2019. These data were queried to determine the total number and sequence of fisheries depletions documented by the historic record. Analysis of this database showed progressive, linearly-increasing exploitation of …

The Effects Of Salt And Temperature On Three Methanogen Species: Implications For Mars, Katy Dunlap

Biological Sciences Undergraduate Honors Theses

The question of whether life has ever existed on Mars – either in the past or currently – has been pursued for decades. This debate has been prompted by a variety of discoveries regarding similarities between Mars and Earth and more recently concerns over human extinction. Mars, like Earth, once had large amounts of water, a key ingredient for life. H₂O exists on Mars in various forms now, and it is theorized that there is liquid water beneath the surface as well. Mars also contains salt and is very cold at its surface, pointing researchers towards the idea …

Effect Of Mating On Male Choosiness In Bicyclus Anynana, Ellie Hope Magera

Biological Sciences Undergraduate Honors Theses

Males mate multiply in many species, including butterflies, but little is known about whether the act of mating influences future male choosiness. We might expect mating to influence future preference, as previous studies have shown that pre-mating social experiences affect future male mate choice. To determine if initial male mate choice affects subsequent male mating decisions, we conducted behavioral assays using the subtropical nymphalid butterfly Bicyclus anynana. We tested three alternative hypotheses: 1) initial mating is a positive experience that strengthens male preference for the previously encountered phenotype; 2) males prefer mating with a different phenotype in their second …

Understanding The Role Of Heterodisulfide Reductase During Nitrogen Fixation By Methanosarcina Acetivorans, Grant Shelnut

Biological Sciences Undergraduate Honors Theses

Methanosarcina acetivorans is a methanogenic archaeon (methanogen) that is important for several processes in our biosphere. A feature of interest within this methanogen is the enzyme nitrogenase which catalyzes the reduction of unusable dinitrogen gas (N₂) into a usable form of nitrogen, ammonia (NH₃). How electrons are transferred within M. acetivorans to nitrogenase during N₂ reduction is unknown. Heterodisulfide reductase (Hdr) is a key enzyme known to reduce the electron carrier protein ferredoxin, which is a direct electron donor to nitrogenase in bacteria. I hypothesized that cytoplasmic HdrA2B2C2 functions in electron transfer to nitrogenase and …

Hormonal Control Of Osmoregulatory Proteins In The Intestine Of Atlantic Killifish (Fundulus Heteroclitus), Karma Hine

Biological Sciences Undergraduate Honors Theses

The Atlantic killifish (Fundulus heteroclitus) is a euryhaline fish, and thus able to maintain ionic and osmotic homeostasis in a wide range of salinities. When exposed to the dehydrating marine environment, the lost water is replenished through drinking seawater (SW) which is processed and absorbed in the intestine through the involvement of various cellular effectors. Intestinal water transport is osmotically driven by the active removal of NaCl from the gut lumen to the blood coupled to the precipitation of divalent Mg²⁺ and Ca²⁺ due to the secretion of bicarbonate. These processes allow for successive removal of …

Understanding Eating Behaviors And The Factors That Affect Them In Caucasian And Ethnic Minority College Students, Warrenesha Arnold

Human Nutrition and Hospitality Management Undergraduate Honors Theses

Many studies have shown that college students do not eat well (Huang et al., 1994; Lorenzoni et al., 2021; Majeed, 2015). Research has identified factors that contribute to the reasons students don’t eat well. Factors such as food preferences (Deliens et al., 2014) eating habits before college (El Ansari et al., 2012), stress (Choi, 2020), dietary knowledge (Deliens et al., 2014; Nani, 2016), availability and accessibility of healthy foods (Payne-Sturges, 2017), knowledge of how to cook (Halfacre et al., 2021) and costs of food (Maroto et al., 2014) contributed to reasons students don’t eat nutritious meals. Dhillon et al. (2019) …

Determining The Functional Roles Of Short Open Reading Frames In Mycobacterium Smegmatis, Lauren Fox

Biological Sciences

The study examines the roles of a subset of short open reading frames (sORFs) in M. smegmatis using targeted mutagenesis and the subsequent examination of phenotypes associated with sORF mutation or overexpression. sORFs are defined as stretches of nucleic acid encoding a protein with a maximum of 50 amino acids. Genome annotation pipelines overlook sORFs encoding small proteins (sproteins) and, thus, sproteins in bacteria have gone unnoticed, even those that are expressed at levels similar to larger, well-described proteins. As a result of recent advances in ribosome profiling and mass spectrometry, hundreds of previously unannotated sORFs have been identified, increasing …

Stabilization Of Pfas Precursors By Surfactant-Modified Clay In Activated Sludge, Monica Quianes

Biological Sciences

PFAS (per- and polyfluoroalkyl substances) and their precursors are environmental contaminants that have been found to be very harmful to both humans and the surrounding environment. These substances are both intentionally used in manufacturing, and also byproducts of other industrial processes, and as a result are found just about everywhere. PFAS virtually do not degrade naturally, so it is necessary for us to find an efficient and cost-effective way to adsorb and/or degrade them manually. Previously tested methods either have not performed well overall or have not performed well in the context of PFAS in WTTP (wastewater treatment plant) activated …

Investigating The Effects Of Vitamin K1 And K2 On The Triple Negative Breast Cancer Cell Line Sum159pt, Monica E. Alexander

Biological Sciences

Vitamin K serves as a cofactor in the post-translational carboxylation of glutamate to γ-carboxyglutamate (GLA) by the enzyme gamma-glutamyl carboxylase (GGCX). Vitamin K epoxide reductases (VKOR) then oxidize the vitamin K to regenerate the compound’s reduced form. While most of the known γcarboxylated proteins function in coagulation and bone homeostasis, the presence of GGCX and VKOR in a wide variety of tissues suggests that the vitamin and its pathway has more extensive physiological roles. Our previous research has assessed the expression of the vitamin K pathway in triple negative breast cancer cells (TNBCs) and compared the effects of vitamin K1 …

Characterization Of Protein-Protein Interactions In Quorum-Sensing Receptors From Gram Negative Bacteria, Jessica Schuh

Biological Sciences

Pseudomonas aeruginosa is a bacterium capable of causing disease in immunocompromised individuals and individuals with underlying lung disorders. It controls the expression of a subset of its genes through quorum sensing, which is a cell-cell communication system involved in mediating the transition from individual to group behaviors. Group behaviors are cellular processes undertaken by the cell that are beneficial to the collective, such as biofilm formation – a key component of pathogenesis in the human lung. They rely on the production, accumulation, detection, and response to signal molecules called autoinducers. There are two acyl-homoserine lactone autoinducer quorum sensing systems in …

Soil Water Use Efficiency Under Integrated Soil Management Practices In The Drylands Of Kenya, Nathan O. Oduor, Monicah Mucheru-Muna, Jayne Njeri Mugwe, Isaya Sijali, George Nyabuga, Daniel Njiru Mugendi

Graduate School of Media and Communications

Soil moisture scarcity and soil fertility decline in the drylands contribute to declining crop productivity. The possible synergistic effects of integrating soil & water conservation, and soil fertility management practices on soil moisture, and hence water use efficiency (WUE) in the drylands of Tharaka-Nithi County in Kenya was assessed. The experiment was laid in a three by three split plot arrangement, with four replications, for four cropping seasons. Minimum tillagewith mulch, tied ridges, and conventional tillage formed the main plot factors. The sub-plot factors included animal manure plus fertilizer at 120, 60, and 30 N kg ha−1. There was significant …

Improving Traumatic Brain Injury Outcomes: Early Identification And Management Of Paroxysmal Sympathetic Hyperactivity, Randi Rockwell

Doctor of Nursing Practice Projects

Abstract

Background: Paroxysmal sympathetic hyperactivity (PSH) can occur in patients with acutely acquired brain injury and is associated with mortality, poor clinical outcomes, prolonged hospitalizations, physical disability, and increased healthcare costs. The most common cause of PSH syndrome is traumatic brain injury (TBI). Symptoms consist of hyperthermia, diaphoresis, arterial hypertension, tachycardia, tachypnea, and extremity motor dystonic posturing, often in response to evoked stimuli and occurs in 8% to 33% of TBI patients. Critical care nurses are at the forefront of providing direct patient care to TBI patients and are often the first to identify PSH.

Purpose: The objective of this …

Quantum Chemistry–Machine Learning Approach For Predicting Properties Of Lewis Acid–Lewis Base Adducts, Hieu Huynh, Thomas J. Kelly, Linh Vu, Tung Hoang, Phuc An Nguyen, Tu C. Le, Emily Jarvis, Hung Phan

Chemistry and Biochemistry Faculty Works

Synthetic design allowing predictive control of charge transfer and other optoelectronic properties of Lewis acid adducts remains elusive. This challenge must be addressed through complementary methods combining experimental with computational insights from first principles. Ab initio calculations for optoelectronic properties can be computationally expensive and less straightforward than those sufficient for simple ground-state properties, especially for adducts of large conjugated molecules and Lewis acids. In this contribution, we show that machine learning (ML) can accurately predict density functional theory (DFT)-calculated charge transfer and even properties associated with excited states of adducts from readily obtained molecular descriptors. Seven ML models, built …

Understanding The Relationship Between B Chromosomes And Nondisjunction In Drosophila Melanogaster, Ayushi Patel

Honors Scholar Theses

B chromosomes are supernumerary, heterochromatic genetic elements that are found in hundreds of different plant and animal species. Recently, B chromosomes were discovered in a stock of Drosophila melanogaster and are carried at a high copy number of 10-12 B chromosomes per cell. B chromosomes are not known to carry any active genes, but when placed in a wild-type genetic background, they cause a significant increase in the frequency of chromosome 4 missegregation during meiosis. This project aimed to understand the relationship between a female’s B chromosome copy number and how often she passes on too many (or too few) …

Modeling Accuracy Matters: Aligning Molecular Dynamics With 2d Nmr Derived Noe Restraints, Milan Patel

Honors Scholar Theses

Among structural biology techniques, Nuclear Magnetic Resonance (NMR) provides a holistic view of structure that is close to protein structure in situ. Namely, NMR imaging allows for the solution state of the protein to be observed, derived from Nuclear Overhauser Effect restraints (NOEs). NOEs are a distance range in which hydrogen pairs are observed to stay within range of, and therefore experimental data which computational models can be compared against. To that end, we investigated the effects of adding the NOE restraints as distance restraints in Molecular Dynamics (MD) simulations on the 24 residue HP24stab derived villin headpiece subdomain to …

Experimental And Observational Research Of Children Physical Activity Intervention: A Synthesis Of The Recent Studies, Yuanlong Liu

International Journal of Physical Activity and Health

No abstract provided.

A Review Of Health Wearable-Based Physical Activity Interventions Among Children And Adolescents, Suryeon Ryu, Pablo Rodriguez-Gonzalez, Zan Gao

International Journal of Physical Activity and Health

Health wearable technologies have become popular in recent decades to improve individual physical activity (PA) behavior. Thus, this paper aims to examine the health of wearable-based PA intervention among children and adolescents and provide narrative overviews. This paper retrieved articles based on the following inclusion criteria: (1) experimental design; (2) studies among healthy children and adolescents aged from 5 to 18 years; (3) studies using health wearables as a medium to enhance PA or reduce sedentary behavior; and (4) studies with PA assessments as an outcome. Relevant articles were searched based on recent preceding review papers published in 2021 and …

Effects Of Active Video Game Interventions On Physical Health And Development Among Healthy Preschool Children: A Systematic Review, Wanjiang Zhou, John Oginni, Juan Jose Palos Perez Sr., Kaitlyn Adams, Zan Gao

International Journal of Physical Activity and Health

Early physical activity (PA) interventions during preschool ages are necessary and critical to cultivate healthy movement behaviors for healthy growth and development. The increasing obesity rate among children is a major public health concern globally, which is largely due to poor adherence to participating in regular PA. Active video game (AVG) interventions have been increasingly used in promoting PA. The narrative review synthesized current literature regarding the effect of AVG interventions on physical health and development indicators among healthy preschoolers. A systematic review was performed in Academic Search Premier, CINAHL, ERIC, PubMed, SPORT Discus, and Web of Science, following the …

A Review On The Dose Response Effect Of Regular Physical Activity On Cognitive Function Among Children And Adolescents, Weidong Li, Ping Xiang

International Journal of Physical Activity and Health

Purpose: Positive effects of physical activity on cognitive function among children and adolescents have been observed in previous studies. However, little is known about whether there is a dose-response effect of physical activity on cognitive functioning. Especially, the curvilinear relationship between regular physical activity and cognitive functioning remained unexplored. The purpose of this paper was to review the literature on the dose response effect of physical activity on cognitive function among children and adolescents. Methods: A literature search on key words, title, and abstract with the phrases “physical activity and executive function”, “physical activity and cognition”, and “physical …

Effect Of Elevated Temperature And Roundup Exposure On Oxidative Stress And Antioxidant Status In The American Oyster, Afsana Chowdhury

Theses and Dissertations

Aquatic organisms are frequently exposed to various environmental stressors. In this study, we observed the short-term (1-week) combined dose-dependent effect of Roundup, a glyphosate-based herbicide, and high temperature (30°C) on the morphology of gills and digestive glands, expression of heat shock protein-70 (HSP70), dinitrophenyl protein (DNP), nitrotyrosine protein (NTP), antioxidant enzymes such as catalase (CAT) and superoxidase dismutase (SOD) in tissues of American oyster (Crassostrea virginica). Histological analyses showed an increase in mucous production in the gills and digestive glands, hemocyte aggregation in the connective tissues as well as structural change of the lumen in the digestive glands …

Sweet Potato In The Lower Rio Grande Valley: Evaluating Performance Across Several Cultivars, Jon Dale

Theses and Dissertations

In Texas’ subtropical, semiarid Lower Rio Grande Valley (LRGV), citrus and sugarcane cultures have dominated specialty crop production for more than a half-century. However, the future of these high-dollar crops is becoming uncertain with increases in exotic pathogens, intensifying urbanization and the overarching complexities of both climate change and binational governance of Rio Grande Basin waters. For these reasons, the region’s specialty crop sector needs to be ready for alternatives that could benefit both market outlets and reduced input dependencies. Sweet potato (Ipomoea batatas), commercially grown in various similar climates worldwide with limited water resources, could be one …

Population Genetics Among Rhipicephalus Sanguineus Ticks In The Lower Rio Grande Valley, Bianca Liana Guerra

Theses and Dissertations

Rhipicephalus sanguineus is a common tick species in the lower Rio Grande Valley of both human and veterinary concern. Two lineages of R. sanguineus have been described across the United States known as the tropical and temperate lineage. Both lineages can be differentiated morphologically, genetically, biologically, and by vector competence. The species name for both lineages has not been well established therefore this thesis will utilize their widely used identification. Both lineages have been identified in Texas. While the distribution of these lineages has been well-defined in some regions across the world, recent studies have observed sympatric populations in certain …

Study Of Population Dynamics Of Sugarcane Aphid (Melanaphis Sacchari) In Rio Grande Valley, Texas, Neetu Khanal

Theses and Dissertations

Chapter 1: This chapter incorporates detailed information about the biotype concept, aphids, their types and their biotypes, importance of studying insect biotypes and their role in mediating host plant defenses.

Chapter 2: This chapter explains in detail about the biology, biotypes, feeding behavior, damage, and economic loss caused due to sugarcane aphid infestation. This chapter further elaborates on the need for studying population level differences and justifies the objectives and significance of this research study.

Chapter 3: This chapter provides information about the comprehensive work done on three different populations of sugarcane aphid collected from three different locations in Rio …

Biological Optimization Of Polymer-Based Antiretroviral Drug Towards The Gut-Associated Lymphoid Tissue (Galt), Arkajyoti Paul

Theses and Dissertations

Background: Combination antiretroviral therapy (cART) has significantly decreased the disease mortality associated with HIV-1 but does not efficiently reach the HIV reservoir organs such as GALT. In this regard, the present study determined the efficacy of the polymer-based nanoformulations using F127 alone and in combination with L61 to encapsulate three clinically available cART drugs such as Emtricitabine (FTC), Tenofovir Disoproxil Fumarate (TDF), and Dolutegravir (DTG) in in vitro and in vivo system. Methods: The selected formulations were studied for their biological and chemical characterization towards human cell line viz. Caco-2 and Human peripheral blood mononuclear cells (PBMC) and in vivo …

Road Mitigation Structure And Road Effect Zone Impacts On Rodent Community Composition, Activity, And Predator-Prey Dynamics In South Texas, Adam F. Sanjar

Theses and Dissertations

The effects of road mortality mitigation structures on rodent activity and rodent community composition are not fully understood, both on the regional scale of south Texas and in the broader field of road ecology. This is primarily due to the low conservation priority of most rodent species and the difficulty of noninvasively surveying for rodents effectively. This thesis focused on characterizing how rodents were impacted by both roads and mitigation structures in south Texas, and if rodents are a significant factor to consider when studying the road ecology of rodent predators in south Texas, particularly ocelots, bobcats (Lynx rufus …

A Programmatic Geographic Information Systems Analysis Of Plant Hardiness Zones, Andrew Bowen

Electronic Theses and Dissertations

The Plant Hardiness Zone Map consists of thirteen geographical zones that describe whether a plant can survive based on average annual minimal temperatures. As climate change progresses, minimum temperatures in all regions are expected to change. This work programmatically evaluates predicted future climate projection data and converts it to United States Department of Agriculture-defined hardiness zones. Through the next 80 years, hardiness zones are projected to move poleward; in effect, colder zones will lose area and warmer zones will gain area globally. Some implications include changes in crop growing degree days, which could alter crop productivity, migration and settlement of …

Structural And Functional Insight Of Helicobacter Pylori Translation Initiation Factor If1 (Hpif1) In Protein Biosynthesis And Rational Design Of New Antimicrobial Candidates, Asif Shahriar

Theses and Dissertations

Helicobacter pylori (H. pylori) is a Gram-negative bacterium that colonizes the human gastric mucosa and is associated with peptic ulcer disease and gastric cancer. Bacterial protein synthesis is a fundamental metabolic process occurring in all bacteria and is a validated target of antibiotics; however, the precise structural mechanism of H. pylori protein biosynthesis remains unknown. Protein synthesis contains four stages, and translational initiation is the most important and highly regulated critical step, involving three initiation factors (IF1, IF2, IF3) and other key components. IF1 plays a vital role in regulating this step by binding to the 30S ribosomal subunit cooperatively …

Red Snapper Abundance, Growth, And Movement At Different Structural Configurations In The Rio Grande Valley Artificial Reef, Marybeth J. Weihbrecht

Theses and Dissertations

The Rio Grande Valley (RGV) artificial reef was built with the goal of providing habitat for reef fish at various life stages. This study used fish trapping to analyze red snapper and grey triggerfish size and abundance in the RGV reef. Mark-recapture of red snapper was utilized to determine growth, movement, and site fidelity within the reef. Nine configurations of patches consisting of varying combinations and densities of concrete pyramids and low-profile modules were surveyed, as well as additional sites of cinder block palettes and concrete railroad ties. Red snapper and triggerfish abundance per trap hour varied significantly by site …