Life Sciences Commons^™

Development And Evaluation Of An Automated Tactical Tillage Tool To Control Weeds In Row-Crop Production Systems, Grace Mccormick Friday

Theses and Dissertations

Weed control is an integral part of a successful overall production strategy in row- cropping systems and has the potential to reduce or eliminate yield losses that negatively affect profitability. Timely and correctly selected herbicide applications are the major keys for effective weed control in a majority of instances. However, there are negative factors that contribute to ineffectiveness and weed escape issues that currently lack viable options for management. Sparsely populated late-season weeds that emerge after lay-by herbicide applications and weeds that have become tolerant and resistant to traditional herbicide chemistries are of greatest concern. Historically, these weeds would have …

Physiological And Proteomic Analysis Of Soybean In Response To Drought And Heat Stress, Amandeep Kaur

Theses and Dissertations

Drought and temperature stress are the two most important factors limiting crop productivity. A physiological and proteomic analysis of soybean was performed to investigate the negative impact of these variables. The previous studies reported the beneficial effect of silicon under stress conditions. Silicon supplementation may promote plant growth under drought stress, although the mechanism to alleviate drought stress is uncertain. The proteomic analysis of chloroplast proteins was carried out to study the response of silicon supplementation under drought stress.

Similarly, temperature stress, mainly heat stress, has an adverse effect on soybean plants. The comparative analysis of two soybean cultivars, DS25-1 …

Physiological And Yield Responses Of Soybean Cultivars To Heat And Drought Stresses, Sadikshya Poudel

Theses and Dissertations

Soybean (Glycine max L.) is an important legume crop often exposed to heat and drought stresses during reproductive and early-seed setting stages, resulting in lower yields and seed quality. Ten soybean cultivars were phenotyped for individual (drought or heat) and combined stress tolerance. Under drought, reduced stomatal conductance and increased canopy temperature significantly reduced seed number (46%) and weight (35%). Heat stress alone reduced seed number (19%) and weight (23%) compared to control. Moreover, a degree increase in daytime temperature above 32 °C during the reproductive stage reduced seed weight by 4% and 7% under well-watered and drought conditions, respectively. …

Molecular Characterization Of Seminal Plasma From Boars With Divergent Sperm Quality, Notsile Hleliwe Dlamini

Theses and Dissertations

Seminal plasma (SP) is the microenvironment of spermatozoa whose composition reflects semen quality and constitutes an excellent source for detecting non-invasive biomarkers. This study characterizes good vs. poor quality semen using seminal extracellular vesicles coupled proteins and miRNA. Fresh boar semen samples were screened, centrifuged, and SP supernatants were collected for near-infrared spectroscopy (NIRS) analysis. EVs were extracted from SP (SP-EVs), characterized, and those of extremely poor or good sperm quality groups were subjected to proteomics and small RNA sequencing. Significant differences were set for P

Interspecies Differences In Organophosphate Anticholinesterase Inhibition Potency And Reactivation Using Novel Oximes, Katie Elizabeth Strickland

Theses and Dissertations

Organophosphates are insecticides which result in acute adverse signs when exposed at toxic doses by animals and lead to death if left untreated. The current treatment for organophosphate toxicity includes atropine and the federally approved oxime 2-PAM. However, 2-PAM is not very effective at crossing the blood brain barrier which results in prolonged inactivation of acetylcholinesterase, which is the primary target of organophosphates, in the brain even after administration. The novel oximes, Oxime 15 and Oxime 20, are able to cross the blood brain barrier and reactivate the inhibited acetylcholinesterase. In this experiment with six animal species frequently used in …

Managing Iron Deficiency Chlorosis (Idc) In Soybean Through A Cropping System Approach, Katelin Savannah Waldrep

Theses and Dissertations

Iron deficiency chlorosis (IDC) is a frequent problem throughout many areas of the United States where soils are high in calcium carbonate (CaCO₃), including the Blackland Prairie regions of Mississippi. The main objectives of this study were to 1) determine the effects of seven different cropping systems on IDC visual symptomology and grain yield in rainfed soybeans grown in calcareous soils, and 2) evaluate the effects of soil water tension (SWT) on IDC. Rotating soybeans with corn produced significantly higher yields for both tolerant and susceptible soybean varieties. IDC symptomology was worse, and yields were lower in cropping …

Impact Of Daily Sweet Potato Ingestion On Alterations To The Gut Microbiome, Cholesterol, And Exercise Performance, Johneric William Smith

Theses and Dissertations

The importance of the gut microbiome is being explored in relation to multiple facets of health and performance. The purpose of this study was to explore the impact of frequent sweet potato ingestion on alterations of the gut microbiome which might in turn alter cholesterol levels and exercise performance. Thirteen recreationally trained males had fecal and blood samples collected and exercised to fatigue with and without exogenous carbohydrate ingestion prior to and following 42 days of daily sweet potato ingestion. Bacterial communities were extracted from fecal samples and bacterial DNA were sequence. Blood samples were analyzed for total cholesterol, low-density …

Genome-Wide Association Study For Agronomic Traits In Bermudagrass (Cynodon Spp.), Lovepreet Singh

Theses and Dissertations

Bermudagrass (Cynodon spp.) breeding and cultivar development is hampered by limited information regarding its genetic and phenotypic diversity. A germplasm collection of 206 bermudagrass accessions from 29 countries was genotyped with high-throughput genotyping-by-sequencing technique. Genomic diversity in this diverse germplasm panel was assessed with multifaceted approaches including population structure, phylogenetic analysis, principal component analysis, and genetic diversity parameters. This study revealed substantial genetic variation in the Cynodon accessions, demonstrating the potential of this germplasm panel for further genetic studies and cultivar development in breeding programs. Another critical issue in turfgrass breeding is the lack of information regarding the genetic …

Evaluation Of Early Maturing Cultivars, Optimal Harvest Timing, And Canopy Reflectance Of Peanut To Maximize Grade And Yield, Joseph Bryan Whittenton

Theses and Dissertations

Peanut digging timing is difficult to predict due to indeterminate growth and peanut pods maturing underground, resulting in the need to research methods that provide consistent measurements, while reducing time and effort for farmers and researchers. Experiments were conducted to evaluate the accuracy of the Maturity Index 1 and Maturity Index 2 in predicting peanut grade, the accuracy of the North Carolina 2 degree day method in predicting peanut yield, and remote sensing vegetative indices sensitivity equivalence (SEq) to peanut Maturity Index 2 and harvest grade (TSMK) for cultivars IPG-914 and Georgia-06G in Mississippi.

Maturity Index 1 and …

Marginal Agricultural Land Identification In The Lower Mississippi Alluvial Valley, Prakash Tiwari

Theses and Dissertations

This study identified marginal agricultural lands in the Lower Mississippi Alluvial Valley using crop yield predicting models. The Random Forest Regression (RFR) and Multiple Linear Regression (MLR) models were trained and validated using county-level crop yield data, climate data, soil properties, and Normalized Difference Vegetation Index (NDVI). The RFR model outperformed MLR model in estimating soybean and corn yields, with an index of agreement (d) of 0.98 and 0.96, Nash-Sutcliffe model efficiency (NSE) of 0.88 and 0.93, and root mean square error (RMSE) of 9.34% and 5.84%, respectively. Marginal agricultural lands were estimated to 26,366 hectares using cost and sales …

An Investigation Into Transcription Fidelity And Its Effects On C. Elegans And S. Cerevisiae Health And Longevity, Olivia S. Dinep-Schneider

Theses and Dissertations

mRNA molecules form an intermediate in the transfer of sequences from DNA to ribosomes in order to guide protein production. Errors can be introduced into mRNA, producing aberrant proteins which place a strain on cellular regulatory machinery, causing increased risks of apoptosis, cancer, and decreased fitness. These errors may be introduced due to decreased transcriptional proofreading capabilities, exposure to chemicals, or mistakes in RNA editing machinery. It is important to investigate these causes of transcription errors to better understand the long-neglected area of mRNA fidelity which has such significant impacts on our cellular functions. In this paper, it was determined …

Determining The Importance Of Propagule Pressure And Dispersal Mechanisms For The Establishment And Spread Of Crapemyrtle Bark Scale, Acanthococcus Lagerstroemiae Kuwana (Hemiptera: Eriococcidae), Erika Renee Wright

Theses and Dissertations

Non-native scale insects can be economically and ecologically important pests of urban forests. Crapemyrtle bark scale (CMBS), Acanthococcus lagerstroemiae Kuwana (Hemiptera: Eriococcidae), is a scale that causes declines in plant vigor for a popular ornamental tree in the southeastern United States, crapemyrtle (Lagerstroemia spp.). CMBS has spread rapidly throughout the Southeast and, more recently, into the Middle Atlantic. Despite problems associated with CMBS, important aspects of its ecology, such as the role of propagule pressure in establishment and the relative importance of mechanisms for between-tree dispersal, are not well-understood. We used field and laboratory studies to investigate these aspects of …

Analysis Of Gene Evolution In The Long-Lived Heliconius Butterfly Genus, Leann Ward

Theses and Dissertations

The butterfly genus Heliconius has an unusually long lifespan (six to eight months compared to only several weeks) and the unique ability to feed on pollen. The additional amino acids provided by a pollen diet seem intrinsically tied to longer lifespan in Heliconius. Because of these traits, Heliconius may be a valuable model species for future aging studies. As a preliminary analysis, we investigated differences in gene evolution between Heliconius and seven short-lived butterflies. We observed that the Heliconius genes had significant differences in evolutionary rates, in the direction of selection, and in the amounts of site-specific positive selection compared …

Remote Physiological Monitoring Of The Giant Panda And Red Panda Using Near Infrared Spectroscopy And Machine Learning, Qingyu Sheng

Theses and Dissertations

Appropriate conservation decisions and efforts must be based on real−time and accurate information about wildlife populations. However, it is extremely challenging to monitor the population demography and physiological traits of many threatened and secretive animal species through direct observation and capture. Near infrared spectroscopy (NIRS) has the potential to be a remote tool to address questions concerning wildlife physiology and demography by analyzing “signs” of animals without seeing or capturing them. In this dissertation, two species, the giant panda (Ailuropoda melanoleuca) and red panda (Ailurus fulgens) are used as a case study, to demonstrate NIRS’ feasibility …

Synthesis Of Biomass-Based Graphene Nanomaterials For Aqueous Heavy Metal Removal And Cement-Based Composite Property Enhancement, Tharindu N. Karunaratne

Theses and Dissertations

Utilizing biomass such as lignin, bamboo, soybean, corn stalk, rice husk, etc., as a carbon source to produce graphene-based nanomaterials has been reported recently. However, the potential of using such nanomaterials for engineering and environmental applications has not been realized. This dissertation investigates the use of graphene-based nanomaterials synthesized from using biomass as a carbon source for water remediation and cement-based composites’ (CBCs) property enhancement.

The first chapter introduces graphene and graphene-based nanomaterials, as well as the synthesis and application of graphene-based nanomaterials for removing heavy metals in an aqueous solution and for property enhancement in CBCs. The experimental investigation …

Macroecological Patterns Of Frugivorous Fishes’ Diversity (Serrasalmidae) In The Amazon Drainage Basin, Karold Vivianna Coronado Franco

Theses and Dissertations

The Amazon River drainage basin is known as the most biodiverse region in the world. Regarding freshwater fish, this region contains around 15% of the world's fish richness. The fish family Serrasalmidae have a variety of feeding preferences including Pacus (herbivores) and Piranhas (carnivores). Fruit eating fish species are evolutionarily the oldest species in the family and have a mutualistic interaction with flooded forests. Considering the diversity of feeding habits of the Serrasalmidae family, it represents an excellent model to study ecological questions related to factors that influence the spatial distribution of species and factors that contribute to the maintenance …

Predict Validity For Prognosis Of Breast Cancer Patients With Pathogenic Brca1/2 Variants, Taru A Muranen, Anna Morra, Sofia Khan, Daniel R Barnes, Manjeet K Bolla, Joe Dennis, Renske Keeman, Goska Leslie, Michael T Parsons, Qin Wang, Thomas U Ahearn, Kristiina Aittomäki, Irene L Andrulis, Banu K Arun, Sabine Behrens, Katarzyna Bialkowska, Stig E Bojesen, Nicola J Camp, Jenny Chang-Claude, Kamila Czene, Peter Devilee, Susan M Domchek, Alison M Dunning, Christoph Engel, D Gareth Evans, Manuela Gago-Dominguez, Montserrat García-Closas, Anne-Marie Gerdes, Gord Glendon, Pascal Guénel, Eric Hahnen, Ute Hamann, Helen Hanson, Maartje J Hooning, Reiner Hoppe, Louise Izatt, Anna Jakubowska, Paul A James, Vessela N Kristensen, Fiona Lalloo, Geoffrey J Lindeman, Arto Mannermaa, Sara Margolin, Susan L Neuhausen, William G Newman, Paolo Peterlongo, Kelly-Anne Phillips, Miquel Angel Pujana, Johanna Rantala, Karina Rønlund, Emmanouil Saloustros, Rita K Schmutzler, Andreas Schneeweiss, Christian F Singer, Maija Suvanto, Yen Yen Tan, Manuel R Teixeira, Mads Thomassen, Marc Tischkowitz, Vishakha Tripathi, Barbara Wappenschmidt, Emily Zhao, Douglas F Easton, Antonis C Antoniou, Georgia Chenevix-Trench, Paul D P Pharoah, Marjanka K Schmidt, Carl Blomqvist, Heli Nevanlinna

Faculty, Staff and Student Publications

We assessed the PREDICT v 2.2 for prognosis of breast cancer patients with pathogenic germline BRCA1 and BRCA2 variants, using follow-up data from 5453 BRCA1/2 carriers from the Consortium of Investigators of Modifiers of BRCA1/2 (CIMBA) and the Breast Cancer Association Consortium (BCAC). PREDICT for estrogen receptor (ER)-negative breast cancer had modest discrimination for BRCA1 carrier patients overall (Gönen & Heller unbiased concordance 0.65 in CIMBA, 0.64 in BCAC), but it distinguished clearly the high-mortality group from lower risk categories. In an analysis of low to high risk categories by PREDICT score percentiles, the observed mortality was consistently lower than …

Home-Site Advantage For Host Species–Specific Gut Microbiota, Daniel D. Sprockett, Jeff Price, Anthony Juritsch, Robert J. Schmaltz, Madalena V. F. Real, Samantha L. Goldman, Michael Sheehan, Amanda E. Ramer-Tait, Andrew H. Moeller

Department of Nutrition and Health Sciences: Faculty Publications

Mammalian species harbor compositionally distinct gut microbial communities, but the mechanisms that maintain specificity of symbionts to host species remain unclear. Here, we show that natural selection within house mice (Mus musculus domesticus) drives deterministic assembly of the house-mouse gut microbiota from mixtures of native and non-native microbiotas. Competing microbiotas from wild-derived lines of house mice and other mouse species (Mus and Peromyscus spp.) within germ-free wild-type (WT) and Rag1-knockout (Rag1^−/−) house mice revealed widespread fitness advantages for native gut bacteria. Native bacterial lineages significantly outcompeted non-native lineages in both WT and Rag1 …

Common Signaling Pathways Involved In Alzheimer's Disease And Stroke: Two Faces Of The Same Coin, Tushar Kanti Das, Bhanu Priya Ganesh, Kaneez Fatima-Shad

Faculty, Staff and Student Publications

Alzheimer's disease (AD) and stroke are two interrelated neurodegenerative disorders which are the leading cause of death and affect the neurons in the brain and central nervous system. Although amyloid-β aggregation, tau hyperphosphorylation, and inflammation are the hallmarks of AD, the exact cause and origin of AD are still undefined. Recent enormous fundamental discoveries suggest that the amyloid hypothesis of AD has not been proven and anti-amyloid therapies that remove amyloid deposition have not yet slowed cognitive decline. However, stroke, mainly ischemic stroke (IS), is caused by an interruption in the cerebral blood flow. Significant features of both disorders are …

The Influence Of Nutrition On The Gut Microbiota And Psychiatric Disorders: A Review, Megha Bhagavan, Francis Jenney

Research Day

The symbiotic harboring of gut microorganisms within the gastrointestinal tract has a profound influence on host physiology, well-being, and disease pathology. More specifically, the gut bacteria are able to interact with dietary components from foods chosen by the host and consequently relay their beneficial or precarious effects locally and systemically. The gut bacteria have the capacity to maintain a healthy gut microbiome or perpetuate intestinal imbalance, known as gut dysbiosis. Gut dysbiosis has both local effects in gastrointestinal pathologies, such as intestinal bowel syndrome (IBS) and intestinal bowel disorder (IBD), as well as systemic pathologies, such as type II diabetes, …

Drug Ideologies Of The United States, Macy Montgomery

Helm's School of Government Conference - 2021-2024

The United States has been increasingly creating lenient drug policies. Seventeen states and Washington, the District of Columbia, legalized marijuana, and Oregon decriminalized certain drugs, including methamphetamine, heroin, and cocaine. The medical community has proven that drugs, including marijuana, have myriad adverse health side effects. This leads to two questions: Why does the United States government continue to create lenient drug policies, and what reasons do citizens give for legalizing drugs when the medical community has proven them harmful? The paper hypothesizes that the disadvantages of drug legalization outweigh its benefits because of the numerous harms it causes, such as …

Mechanistic Examination Of Protist-Mediated Plant Growth Through The Comparative Development Of Medicago Truncatula, Shane Connolly

University Scholar Projects

Protists are known to increase plant growth through two main mechanisms: the microbial loop and the alteration of the root microbiome. The microbial loop is a nutrient recycling method in which protists provide inorganic nitrogen ions to the plant. Alteration of root microbiome leads to the removal of plant pathogens and shifting communities towards plant growth-promoting bacteria (PGPB). This study aimed to elicit which mechanism could produce the largest boost in shoot weight for Medicago truncatula. A series of microcosm experiments were explored in which M. truncatula was grown with variable microbiome structures to allow for mechanism differentiation. The …

Cenabis Bene: A Culinary Odyssey Through Apicius, Kathryn Atkinson

University Scholar Projects

Apicius is the sole surviving cookbook from classical antiquity; as such it is invaluable for what it tells us about ancient feasting customs. Yet the gluttony typically associated with classical antiquity has no place in Apicius beyond the art that is inherently associated with food; we are not so much given a seat at the cena (dinner) as we are led into the kitchen, handed an apron, and instructed to cook. This critical analysis explores each recipe not only on the surface—i.e., examining the ingredients and recreating selected recipes—but also on a deeper level, lifting food above its concrete reality …

Synecological Response Of Spring Benthic Prokaryotes And Macroinvertebrates To Paleozoic Roof Pendant-Derived Calcium, Ariel D. Friel, Khaled Pordel, Zachary Meyers, Cale O. Seymour, Nicole J. Thomas, Fred M. Phillips, Jeffrey R. Knott, Donald W. Sada, Laura Rademacher, Marty Frisbee, Brian P. Hedlund

Pacific Faculty Work

Despite the ecological significance of desert springs, little is known about relationships between spring hydrogeochemistry and ecology, particularly over multiple trophic levels. Here, we surveyed microbial communities (bacteria and archaea) and benthic macroinvertebrate (BMI) communities in springs that recharge through granitoid bedrock in Owens Valley, CA, to determine whether subtle geochemical differences imparted by metamorphosed roof pendant weathering in the source area affect spring ecosystems. Relative to other springs, roof-pendant-recharged springs were characterized by elevated (1) Ca²⁺/Na⁺, Ca²⁺/Mg²⁺, and divalent/monovalent cation ratios, (2) relative abundance of taxa inferred to be benthic aerobes …

The Last Of Us In Therapy: How Mind-Controlling Fungi And Gut Bacteria Affect Your Mental Health, Anastasia Lyon

Journal of Pharmacology & Nutritional Sciences

The "psilocybiome" represents the mutually beneficial relationship between ourselves, our bacteria, and psychedelic drugs. This short review briefly discusses the benefits and limitations surrounding the potential for psychedelic therapy to synergize with gut bacteria to help regulate and maintain proper balance in the immune system, diet, and stress levels. Psychedelic therapy is a novel treatment strategy that has the potential to improve patient mental health, and, by identifying the types of gut bacteria present in patients, it can aid in personalizing medicine by determining how well their "psilocybiome" may respond.

Synecological Response Of Spring Benthic Prokaryotes And Macroinvertebrates To Paleozoic Roof Pendant-Derived Calcium, Ariel D. Friel, Khaled Pordel, Zachary Meyers, Cale O. Seymour, Nicole J. Thomas, Fred M. Philips, Jeffrey R. Knott, Donald W. Sada, Laura Rademacher, Marty Frisbee, Brian P. Hedlund

Life Sciences Faculty Research

Despite the ecological significance of desert springs, little is known about relationships between spring hydrogeochemistry and ecology, particularly over multiple trophic levels. Here, we surveyed microbial communities (bacteria and archaea) and benthic macroinvertebrate (BMI) communities in springs that recharge through granitoid bedrock in Owens Valley, CA, to determine whether subtle geochemical differences imparted by metamorphosed roof pendant weathering in the source area affect spring ecosystems. Relative to other springs, roof-pendant-recharged springs were characterized by elevated (1) Ca2+/Na+, Ca2+/Mg2+, and divalent/monovalent cation ratios, (2) relative abundance of taxa inferred to be benthic aerobes and prosthecate/stalked bacteria, and (3) abundance and diversity …

Coloration Of Western Alaskan Lakes From Spectral Data Correlates With Environmental Factors, Annemarie Timling

Undergraduate University Honors Capstones

The Yukon Kuskokwim Delta region of Alaska consists of over 100,000 small lakes; it is one of the largest lake-dense regions of Alaska. These small lakes are critical aquatic ecosystems that provide habitats for wildlife and play an important role in carbon and nutrient cycling. Lakes in the Yukon Kuskokwim Delta have a wide range of visible and near-infrared satellite spectra, which can vary over small spatial scales. These spectral differences may provide insight into the environmental properties of these small shallow lakes. Therefore, we want to investigate the relationship between the spectral data and environmental differences in these lakes. …

Human Trafficking And Nutrition: Assessing The Effects Of U.S. Public Policy On Food Security And The Malnourishment Of Refugees, Moxie R. Mccandless

Honors Projects

This research paper aims to assess the challenges associated with human trafficking within the lived experience of refugees, specifically focusing on food security, and the resulting system shaped by existing United States policies and programs. By examining this complex relationship, the study seeks to shed light on the influence of power dynamics on fulfilling basic human needs and to propose more effective strategies for improving food security among marginalized populations.

A comprehensive literature review is conducted, and a community nutrition needs assessment is performed in King County, Washington. Qualitative interviews are conducted with human trafficked organizations, government officials, and experts …

Effects Of Increased Shoe Collar Height And Limb Dominance On Landing Knee Biomechanics In Collegiate Volleyball Players, Lindsey Legg

Master's Theses

Athletic footwear with higher collar heights are worn to restrict ankle motion. Reduced ankle dorsiflexion has been associated with increased frontal plane knee motion. Volleyball players wear mid-cut shoes (MC) that have an increased collar height rising slightly superior to the talocrural joint and malleoli. The purpose of this study was to determine the effect of MC and limb dominance on knee landing mechanics. It was hypothesized that participants would land with greater initial contact (IC) and peak frontal joint plane angles and moments and smaller IC and peak sagittal plane joint angles and moments at the knee in MC …

Comparative Analysis Of Rhizosphere And Endosphere Microbiome Of Different Blueberry Species (Vaccinium Sp.), Niladri Bhowmik

Master's Theses

Blueberries are an important agricultural commodity in all over the United States. Due to its health benefits, there is a huge demand globally, thus expanding the industry. Breeding programs are essential to maintain such industries. Challenges that play a role in contemporary breeding programs are various biotic and abiotic stress factors. Studies have shown that microorganisms are recruited by plants to alleviate them during stressful conditions. Though blueberries have been cultivated for about 100 years, how the microbiome has been affected due to this is poorly understood. We hypothesized that interspecific crosses and artificial selection have significantly changed the microbiome …