Life Sciences Commons^™

The Unique Gut Microbiome Of Giant Pandas Involved In Protein Metabolism Contributes To The Host's Dietary Adaption To Bamboo, Feilong Deng, Chengdong Wang, Desheng Li, Yunjuan Peng, Linhua Deng, Yunxiang Zhao, Zhihao Zhang, Ming Wei, Kai Wu, Jiangchao Zhao, Ying Li

Animal Science Faculty Publications and Presentations

Background

The gut microbiota of the giant panda (Ailuropoda melanoleuca), a global symbol of conservation, are believed to be involved in the host’s dietary switch to a fibrous bamboo diet. However, their exact roles are still largely unknown.

Results

In this study, we first comprehensively analyzed a large number of gut metagenomes giant pandas (n = 322), including 98 pandas sequenced in this study with deep sequencing (Illumina) and third-generation sequencing (nanopore). We reconstructed 408 metagenome-assembled genomes (MAGs), and 148 of which (36.27%) were near complete. The most abundant MAG was classified as Streptococcus alactolyticus. A …

Nivolumab And Ipilimumab In Combination With Radiotherapy In Patients With High-Risk Locally Advanced Squamous Cell Carcinoma Of The Head And Neck., Jennifer Johnson, Ioannis A. Vathiotis, Larry Harshyne, Ayesha Ali, Voichita Bar-Ad, Rita S. Axelrod, Emily Lorber, Joseph Curry, David Cognetti, Adam J. Luginbuhl, Madalina Tuluc, Scott W Keith, M.G. Mahoney, Athanassios Argiris

Department of Medical Oncology Faculty Papers

BACKGROUND: The combination of nivolumab and ipilimumab has been approved for the treatment of multiple solid tumors. This was a phase I study investigating definitive radioimmunotherapy (RIT) with nivolumab and ipilimumab for the treatment of locally advanced (LA) squamous cell carcinoma of the head and neck (SCCHN).

METHODS: Patients with newly diagnosed, stage IVA-IVB SCCHN eligible for cisplatin-based chemotherapy received nivolumab (3 mg/kg every 2 weeks for a total of 17 doses) and ipilimumab (1 mg/kg every 6 weeks for a total of 6 doses) starting 2 weeks prior to radiotherapy. The primary endpoint was safety of definitive RIT. Secondary …

Assessment Of Integrated Nutrient Management Practices On Soil Health And Nitrogen Efficiency In Cropping Systems, María José Oviedo Ventura

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Groundwater quality in Nebraska is threatened by non-point source contributions of nitrate-nitrogen, primarily by agricultural systems. Intensive crop and livestock production that neglects the value of manure in crop fertility plans can contribute to soil degradation and natural resource impairment. The purpose of this study is to evaluate opportunities to improve agricultural nutrient management throughout Nebraska by quantifying opportunities for manure distribution, evaluating soil health implications of organic and inorganic soil amendments, and assessing nutrient management practices focused on reducing nitrate leaching in agricultural soils.

This study described in Chapter 2 provides a spatial illustration of nitrogen and phosphorus balances …

Influence Of Overcooking On Food Digestibility And In Vitro Fermentation, Wensheng Ding

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Areas of char or overcooking commonly appear in foods people consume. It has been reported that overcooked food is harmful to human health. However, little research exists on the effect of overcooking on in vitro protein and starch digestibility and gut microbial fermentation. This study aimed to reveal the connection between overcooking and in vitro protein and starch digestibility and gut microbial fermentation. In vitro protein digestibility of an overcooked ground beef patty was almost half that of a standard cooked sample (27 ± 2% versus 48 ± 6%, respectively; p = 0.02). Whole wheat bread protein digestibility was also …

Effects Of Circadian Rhythm Disruption And Social Jetlag On The Cardiometabolic Adaptations To Exercise, Michael Bevan Dial

UNLV Theses, Dissertations, Professional Papers, and Capstones

Circadian rhythms lie within every tissue and cell and regulate cellular and tissue processes and functions based upon the time of day. Disrupting the circadian clock results in impaired tissue and organ activity and higher risk of cardiometabolic diseases. A common form of circadian rhythm disruption is social jetlag (SJL), which represents the misalignment of sleep and wake times on free days and work days (i.e. weekend). The time of day of exercise affects the adaptations that are derived from exercise. Within the heart muscle, disparate levels of exercise-induced hypertrophy, circadian clock gene expression, and mitochondrial content were all dependent …

Understanding The Distribution And Abundance Of Asclepias Prostrata In South Texas, Elizabeth Ann Gonzalez

Theses and Dissertations

Asclepias prostrata, commonly referred to as prostrate milkweed, is a rare South Texas native plant species. In February of 2023, A. prostrata was listed as a federally endangered species by the U.S. Fish and Wildlife Service. Due to the limited available observations and literature for A. prostrata, this study was completed to bring new information that could be utilized for future restorative efforts. This study includes multiple field observations of the plant’s morphology, plant-insect interactions, extraction and transplanting of the species, and a seed viability study. Results indicate that although A. prostrata has many pressures that limit its …

Lunchbox Hydroponics, Stacy A. Adams

Department of Agronomy and Horticulture: Faculty Publications

Growing plants with hydroponics is less complicated than it may appear when the fundamentals of the system are understood. This publication provides instruction on the construction of a simple hydroponic prototype suitable for school or youth education programs. The fundamentals of hydroponics, basic design of a solution system, seed starting, creating a nutrient solution, and plant care are explored through this engaging project. This prototype can successfully be used to grow small edible and herb plants in the classroom or at home.

Song Structure Of Male Northern House Wrens And Patterns Of Song Production And Delivery Across The Nesting Cycle, Rachael A. Disciullo, Scott K. Sakaluk, Charles Thompson

Faculty Publications – Biological Sciences

Studies of the role of bird song in avian communication have revealed numerous functions across many species, including its critical importance in female mate choice (intersexual selection) and male-male competition (intrasexual selection). A first step in understanding the role of song in different contexts is to describe the note, syllable, and song-types that occur, as well as their patterns of production within a population. Here, we do so for a north-central Illinois, USA, population of Northern House Wrens (Troglodytes aedon). As has been described in other populations, most songs (≈ 1–3 s in duration) contain an introductory section of soft, …

Topical, Contact, And Oral Susceptibility Of Adult Culicoides Biting Midges (Diptera: Ceratopogonidae) To Fluralaner, Blythe E. Lawson, Emily G. Mcdermott

Entomology and Plant Pathology Faculty Publications and Presentations

Background

Culicoides biting midges (Diptera: Ceratopogonidae) are economically important blood-feeding pests closely associated with livestock production. They are the principal vectors of two hemorrhagic disease viruses affecting both wild and domestic ruminants within the US: bluetongue virus (BTV) and epizootic hemorrhagic disease virus (EHDV). BTV impacts the US agriculture sector through direct commodity loss and strict international livestock trade restrictions. Yet, despite posing a considerable threat to US livestock, Culicoides are understudied, and management strategies are lacking. Current control tools for Culicoides are limited to synthetic chemicals, predominantly pyrethroids. With limited products available for livestock producers, proper pesticide rotation is …

Age-Related Differences In Motor Performance, Jessica Anne Prebor

Rehabilitation Sciences Theses & Dissertations

The purpose of this work was to study the age effects on average performance and variability of movement responses in children, young adults, and older adults across multiple motor tasks. Optimal motor performance is observed in healthy young adults with declines observed at either end of the lifespan. This pattern has been represented as a U-shaped/inverted U-shaped curve. Little is known about if this pattern persists in chewing dynamics. While chewing has been found to improve aspects of attention, a cognitive function, research is limited on the relationship between chewing and other motor tasks.

The first aim of this research …

Tidal Flooding In The Mid-Atlantic Region Of The Us: Water Quality Effects In The Lower Chesapeake Bay, Alfonso Macias Tapia

OES Theses and Dissertations

Many coastal areas around the globe suffer from nutrient pollution and its environmental, social, and economic consequences. Nutrient inputs can come from point (e.g., the end of a pipe) and nonpoint sources, from which the former are better constrained as sampling need only be conducted at a discharge point. Given the temporal and spatially extensive nature of tidal flooding events, they can represent another type of nonpoint source of nutrients to adjacent water bodies heretofore, unexamined and quantified. Most studies examining impacts of tidal flooding have focused on threats to resources on land, such as urban infrastructure and human health …

Illuminating Underappreciated Mechanisms Of Receptor Regulation In Human Lymphocytes., Cassandra Woolley

Electronic Theses and Dissertations

A thorough understanding of receptor regulation is imperative to predict expression in varying contexts of disease or treatment. Lymphocyte surface receptors are often used as biomarkers and drug targets, making them particularly important for study. For receptors of debated functionality, such as the Fc receptor for IgM (FcMR), understanding regulation can also help to predict expression in vivo to supplement hypotheses of biological roles. Various mechanisms exist for altering receptor surface expression, including direct feedback mechanisms such as ligand-induced endocytosis and broader mechanisms such as transcriptional and translational control. In this dissertation, we explore selected underappreciated mechanisms of lymphocyte receptor …

New Species Of Dryolestoid From The Late Cretaceous Allen Formation And Implications For South American Faunal Diversity., Brigid Erin Connelly

Electronic Theses and Dissertations

Dryolestoids are extinct cladotherians mammals from the Jurassic and Cretaceous periods. I describe a collection of dryolestoid specimens from the Late Cretaceous localities of Cerro Tortuga (Allen Formation), Anfiteatro 1, and Shining (both La Colonia Formation) from Patagonia, Argentina. Using comparative morphology, I identify a new species of meridiolestidan dryolestoid based on eleven specimens across both formations. The new species’ recovery from La Colonia Formation represents the first dryolestoid connection between the two approximately contemporaneous formations. The species’ morphology may represent an ecological shift within Meridiolestida from insectivory to herbivory, showing a transition in characters between the plesiomorphic sharp-toothed meridiolestidans …

Epitranscriptomic Regulation In Breast Cancer And Pcb-Induced Liver Disease., Belinda Petri

Electronic Theses and Dissertations

Post-transcriptional RNA modifications including N6-methyladenosine (m6A) regulate mRNA stability, splicing, and translation. My research examined m6A in two disease models: breast cancer (BCa) and non-alcoholic fatty liver disease (NAFLD). Acquired resistance to endocrine therapies (ET) develops in approximately 20% of BCa patients with estrogen receptor α positive (ER+) tumors following treatment. The mechanisms by which tumor cells evade ET are not completely understood. Using a cell line model, we investigated the role of an m6A reader protein, HNRNPA2B1 (A2B1) that is upregulated in ET-resistant ER+ BCa cells. Stable overexpression of A2B1 in ET-sensitive MCF-7 cells (MCF-7-A2B1), results in ET resistance, …

Measurement Of Conduction, Radiation, And Convection Thermal Energy To Assess Baking Performance In Residential Ovens., Sean Simpson

Electronic Theses and Dissertations

Residential ovens are complicated thermal environments capable of delivering convection, conduction, and radiation heat transfer to food. The amount and mode of heat transfer can change based on the design of the oven cavity, cooling systems, and oven cycle algorithms. Studies have shown that the changes in one heat transfer mode can have an impact on the quality of baked goods. The many variables involved make designing residential ovens a time consuming and costly process. The goal of this study is to adapt thermal energy sensing technology to collect energy data from a residential oven and develop correlations to quality …

Fbg Αc 389 – 402 Modulates Factor Xiii Crosslinking In The Fibrinogen Αc Region., Francis Dean Orlina Ablan

Electronic Theses and Dissertations

Fibrinogen (Fbg) is a coagulation protein critical for clot formation. Coagulation Factor XIII (FXIII) is a calcium-dependent transglutaminase that crosslinks reactive glutamines (Q) and lysines (K) between fibrin and other anti-fibrinolytic proteins. In the presence of Ca²⁺, FXIII could be activated non-proteolytically (FXIII-A°), or proteolytically by thrombin (FXIII-A*). Significant increases in clot stability and red blood cell retention are linked to FXIII activity in the fibrinogen αC region (Fbg Aα 221 – 610). This region contains several FXIII-reactive glutamines and lysines, as well as a binding site for FXIII-A* (Fbg αC 389 – 402) that includes a key …

Identification Of Genetic Variation Influencing Metformin Response In A Multiancestry Genome-Wide Association Study In The Diabetes Prevention Program (Dpp), Josephine H Li, James A Perry, Kathleen A Jablonski, Shylaja Srinivasan, Ling Chen, Jennifer N Todd, Maegan Harden, Josep M Mercader, Qing Pan, Adem Y Dawed, Sook Wah Yee, Ewan R Pearson, Kathleen M Giacomini, Ayush Giri, Adriana M Hung, Shujie Xiao, L Keoki Williams, Paul W Franks, Robert L Hanson, Steven E Kahn, William C Knowler, Toni I Pollin, Jose C Florez, Diabetes Prevention Program Research Group

Children’s Nutrition Research Center Staff Publications

Genome-wide significant loci for metformin response in type 2 diabetes reported elsewhere have not been replicated in the Diabetes Prevention Program (DPP). To assess pharmacogenetic interactions in prediabetes, we conducted a genome-wide association study (GWAS) in the DPP. Cox proportional hazards models tested associations with diabetes incidence in the metformin (MET; n = 876) and placebo (PBO; n = 887) arms. Multiple linear regression assessed association with 1-year change in metformin-related quantitative traits, adjusted for baseline trait, age, sex, and 10 ancestry principal components. We tested for gene-by-treatment interaction. No significant associations emerged for diabetes incidence. We identified four genome-wide …

Factors Associated With Choice Of Behavioural Weight Loss Program By Adults With Obesity, Yasaman Jamshidi-Naeini, Susan B Roberts, Stephanie Dickinson, Arthur Owora, Jon Agley, Roger S Zoh, Xiwei Chen, David B Allison

Children’s Nutrition Research Center Staff Publications

We assessed the preference for two behavioural weight loss programs, Diabetes Prevention Program (DPP) and Healthy Weight for Living (HWL) in adults with obesity. A cross-sectional survey was fielded on the Amazon Mechanical Turk. Eligibility criteria included reporting BMI ≥30 and at least two chronic health conditions. Participants read about the programs, selected their preferred program, and answered follow-up questions. The estimated probability of choosing either program was not significantly different from .5 (N = 1005, 50.8% DPP and 49.2% HWL, p = .61). Participants' expectations about adherence, weight loss magnitude, and dropout likelihood were associated with their choice (p <  .0001). Non-White participants (p = .040) and those with monthly income greater than $4999 (p = .002) were less likely to choose DPP. Participants who had postgraduate education (p = .007), did not report high serum cholesterol (p = .028), and reported not having tried losing weight before (p = .025) were more likely to choose DPP. Those who chose HWL were marginally more likely to report that being offered two different programs rather than one would likely affect their decision to enrol in one of the two (p = .052). The enrolment into DPP and HWL was balanced, but race, educational attainment, income, previous attempt to lose weight, and serum cholesterol levels had significant associations with the choice of weight loss program.

Consistency Is Key: Investigating Vocal Consistency In Field Sparrows, Stephanie Stanton

Masters Theses

No abstract provided.

Investigating The Effects Of E219 Mutations In M. Tuberculosis Indole-3-Glycerol Phosphate Synthase, Cinthya Moran

Theses, Dissertations and Culminating Projects

Tuberculosis (TB) is a serious infectious disease caused by Mycobacterium tuberculosis. Tuberculosis drug resistance is increasingly a problem, but recent research has provided results that suggest Indole-3-Glycerol Phosphate Synthase (IGPS) could be a potential drug target. IGPS is an enzyme that catalyzes the fourth step in the tryptophan biosynthesis pathway, a crucial process for optimal Mycobacterium tuberculosis growth. In this research, we studied IGPS from Mycobacterium tuberculosis (MtIGPS) to shed light on a particular conserved residue that has not been studied in depth in any IGPS homolog. My investigation focuses on understanding the role of the active site residue E219. …

Density And Occupancy Of Mammals Along An Urban To Rural Gradient In Northwest Arkansas, Leah E. Mctigue

Graduate Theses and Dissertations

Human development is a rapidly expanding phenomenon impacting wildlife communities globally. As the human footprint expands, natural greenspace and nature reserves become fragmented and isolated. Wildlife species and communities respond to these changes in different ways. Urban exploiters persist in more urban areas and are able to take advantage of anthropogenic subsidies (food, shelter, etc). Urban avoiders, however, are species that may be pushed out of developed areas or occur in lower densities relative to undisturbed areas. Northwest Arkansas is an optimal location to study the impacts of urbanization on wildlife communities, as the current human population is expected to …

Explicit And Implicit Measurements Of Sensory And Emotional Responses To Stimuli In Children With Autism Spectrum Disorder, Asmita Singh

Graduate Theses and Dissertations

Autism Spectrum Disorder (ASD) is a developmental disability that causes significant social, communication, and behavioral challenges and it is often involved in sensory processing issues that lead to feeding problems. While our understanding of how sensory responses differ in people with ASD is limited, past studies indicated atypical responses to certain stimuli. If these stimuli can be identified, they could be good indicators to identify the risk of autism in young children. The main objective of this dissertation was to identify a specific stimulus leading autistic children to exhibit atypical responses in terms of sensory or emotional aspects. Children between …

The Influence Of Temperature, Prey Availability, And Population Of Origin On Ectotherm Energetics, Allison R. Litmer

Graduate Theses and Dissertations

Climate change poses threats to organisms regarding direct and indirect effects of warming temperatures. Laboratory studies and predictive models are useful for understanding how temperature influences organisms, and the future of species’ life history and persistence. This dissertation expands upon current approaches to thermal studies and climate models, while investigating effects of warming nighttime temperature and altered prey availability on individuals and populations. Lizards in the genus Sceloporus were used as model organisms, as they are broadly distributed with thermally sensitive physiologies. The first dissertation chapter conducted meta-analyses to examine temperature-induced changes in food consumption, activity, and life history. Prominent …

The Effect Of Carbohydrate Source As Part Of A High Protein, Weight-Maintenance Diet On Outcomes Of Well-Being In Adults At Risk For Metabolic Syndrome, Mariya Hayat

Graduate Theses and Dissertations

Obesity is a growing epidemic affecting at least 312 million people worldwide and is a major risk factor for chronic diseases such as metabolic syndrome (MetS). MetS is defined based on the National Cholesterol Education Program Adult Treatment Panel III, as having 3 or more of the following: waist circumference greater than 102 cm in men or greater than 88 cm in women; serum level of triglycerides of 150 mg/dL or greater; high-density lipoprotein (HDL) cholesterol level of less than 40 mg/dL in men or less than 50 mg/dL in women; systolic/diastolic blood pressure of 130/85 mmHg or greater or …

Rice Material State Diagrams: Trends Of Contemporary Cultivars And Influence Of Nitrogen Application During Production, Evans Ameyaw Owusu

Graduate Theses and Dissertations

Rice is typically harvested at high moisture content (MC) levels which necessitates drying to low MC levels for safe storage. However, drying at inappropriate temperatures and durations can lead to deterioration of kernel quality such as fissure formation. Kernels with these fissures are typically associated with reduced head rice yields and significant economic losses. Material state diagrams for rice have been created by the University of Arkansas Rice Processing Program (UARPP) as a tool to aid in predicting the ideal temperatures to use during drying and tempering (a process of holding rice at the drying temperature for some duration). The …

The Influence Of Climate And Landscape On Allocapnia Mohri Distribution In Arkansas, Brianna Leigh Annaratone

Graduate Theses and Dissertations

Stoneflies are a globally threatened aquatic insect order. In Arkansas, a diverse group of winter stonefly (Capniidae: Allocapnia) have not been surveyed since the 1980s, likely because species-level identification requires the rarely-collected adult form. Allocapnia mohri, a regional endemic, was previously commonly found in mountainous, intermittent streams from the Ouachita Mountains ecoregion north to the Ozark Highlands, but no species distributional models including land use or climate variables exist to our knowledge. We collected adults from 70 stream reaches from the historic Arkansas range from November to April 2020 and 2021. We modeled distributions using random forest (RF) models populated …

The Effect Of Herbal Adaptogen On Feeding-Related Hypothalamic Neuropeptides In Heat-Stressed Broilers, Maryam Afkhami Ardakani

Graduate Theses and Dissertations

Heat stress is a major issue in the poultry industry, having numerous negative consequences for chicken productivity. Decreasing feed intake is one of these issues, which results in higher mortality and poor growth performance. Moreover, Herbal adaptogens, plant extracts that are also known as stress response modifiers, are metabolic regulators that improve an organism's ability to adapt to environmental stresses and minimize damage from such stressors. Previously, we published data indicating that herbal adaptogen supplementation increased feed intake under heat stress conditions. Therefore, we hypothesized that dietary herbal adaptogen supplementation might exert these effects through alteration of the expression of …

Electrochemically Precipitated Struvite Effects On Soil Property And Crop Response, Tatum Simms

Graduate Theses and Dissertations

Phosphorus (P) is a vital resource that is finite and only mineable in raw-material form in a few countries worldwide. Struvite (MgNH4PO4·6H2O) is a mineral that can be produced from wastewater that may provide a promising alternative source of fertilizer-P for crop production, but comprehensive characterizations of struvite behavior in various soils and responses of various crops are necessary. This study aimed to determine the (1) leaching potential, (2) vertical soil profile-P distribution, (3) wheat (Triticum aestivum), corn (Zea mays), and soybean (Glycine max) response, and (4) soil property changes over time of electrochemically precipitated struvite (ECST) compared to that …

Anti-Inflammatory Effect Of Apiaceous Vegetables In A Mouse Model Of Colitis - Implications For Colon Cancer Risk, Laura Mariana Mariana Echavarri Ibarra

Graduate Theses and Dissertations

Colorectal cancer is the second leading cause of cancer-related deaths in the US. Dietary habits account for 50% of the cases. A westernized diet (high fat, high sugar/fructose, and low in fruits and vegetables) and inflammatory bowel diseases are risk factors for colorectal cancer. Evidence suggests phytochemicals in apiaceous vegetables (API; celery family) possess anti-inflammatory and potentially chemopreventive properties. Hence, investigation of whether the consumption of API mitigates acute colitis is warranted. Using mice fed the Total Western Diet (TWD) and administered dextran sodium sulfate (DSS; induces colitis), the objectives of this project are to identify the effects of API …

Use Of X-Ray Imaging To Elucidate Impacts Of Drying Approach On Rice Seed Health, Samuel Olayiwola Olaoni

Graduate Theses and Dissertations

Improper drying, which causes fissuring of rice kernels, may influence the overall seed health. This study evaluated the impacts of various drying conditions on rice fissures, seed germination and vigor. A long – grain rice cultivar with a moisture content (MC) of 15.5% w.b. (wet basis) was used during the experiments. Samples were dried as thin layers at temperatures of 30 ℃, 40 ℃ and 50 ℃ with a constant relative humidity (RH) of 50% and air velocities (AV) of 0.66 m/s and 1.65 m/s. X – ray imaging was used for fissure detection before and after drying the samples …