Life Sciences Commons^™

Knowledge Representation And Management 2022: Findings In Ontology Development And Applications

Faculty, Staff and Student Publications

OBJECTIVES: To select, present, and summarize the best papers in 2022 for the Knowledge Representation and Management (KRM) section of the International Medical Informatics Association (IMIA) Yearbook.

METHODS: We conducted PubMed queries and followed the IMIA Yearbook guidelines for performing biomedical informatics literature review to select the best papers in KRM published in 2022.

RESULTS: We retrieved 1,847 publications from PubMed. We nominated 15 candidate best papers, and two of them were finally selected as the best papers in the KRM section. The topics covered by the candidate papers include ontology and knowledge graph creation, ontology applications, ontology quality assurance, …

Identifying Contributing Factors Associated With Dental Adverse Events Through A Pragmatic Electronic Health Record-Based Root Cause Analysis, Emily Sedlock, Yan Xiao, Janelle Urata, Greg Olson, Amy Franklin, Krishna Kookal, Ana Ibarra-Noriega, Sayali Tungare, Oluwabunmi Tokede, Heiko Spallek, Joel M White, Muhammad F Walji

Faculty, Staff and Student Publications

OBJECTIVE: This study assessed contributing factors associated with dental adverse events (AEs).

METHODS: Seven electronic health record-based triggers were deployed identifying potential AEs at 2 dental institutions. From 4106 flagged charts, 2 reviewers examined 439 charts selected randomly to identify and classify AEs using our dental AE type and severity classification systems. Based on information captured in the electronic health record, we analyzed harmful AEs to assess potential contributing factors; harmful AEs were defined as those that resulted in temporary moderate to severe harm, required hospitalization, or resulted in permanent moderate to severe harm. We classified potential contributing factors according …

Quantifying The Impacts And Assessing The Permeability Of A Divided Four-Lane Highway On Migratory Mule Deer, Daniel P. Taylor

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Increased human development and expansion has led to an ever-growing transportation network, which has both direct and indirect ecological impacts to local wildlife populations. In response, wildlife crossing structures have become an increasingly fundamental component of wildlife conservation throughout the United States. The focus of this study was a 13.5 km section of US Highway 395, Mono County, California. The California Department of Transportation recently identified this section of highway, hereafter known as the Mammoth Wildlife Crossing, as the highest priority location for wildlife crossing structures along US 395 within Mono County. The goal of this study was to use …

Sulfur Cycling Connects Microbiomes And Biogeochemistry In Deep-Sea Hydrothermal Plumes, Zhichao Zhou, Patricia Q. Tran, Alyssa M. Adams, Kristopher Kieft, John A. Breier, Caroline S. Fortunato, Cody S. Sheik, Julie A. Huber, Meng Li, Gregory J. Dick, Karthik Anantharaman

School of Earth, Environmental, & Marine Sciences Faculty Publications

In globally distributed deep-sea hydrothermal vent plumes, microbiomes are shaped by the redox energy landscapes created by reduced hydrothermal vent fluids mixing with oxidized seawater. Plumes can disperse over thousands of kilometers and their characteristics are determined by geochemical sources from vents, e.g., hydrothermal inputs, nutrients, and trace metals. However, the impacts of plume biogeochemistry on the oceans are poorly constrained due to a lack of integrated understanding of microbiomes, population genetics, and geochemistry. Here, we use microbial genomes to understand links between biogeography, evolution, and metabolic connectivity, and elucidate their impacts on biogeochemical cycling in the deep sea. Using …

Sequestered Sequences: A Bioinformatic Approach To The Forgotten Genome, Dylan Barth

UNLV Theses, Dissertations, Professional Papers, and Capstones

As high throughput sequencing generates ever increasing amounts of genetic and epigenetic data new lines of inquiry open up in the field of genomic research. In this thesis, we discuss three ways in which we can utilize public databases of next generation genomic data in order to study areas of the genome previously ignored by traditional approaches. These include the study of linker regions between domains of proteins, indirect enhancers that do not strongly contact promoters of genes they regulate, and transposon-derived enhancer elements. The work uncovers many exceptions to known biological principles, and adds nuance to our understanding of …

The Genetic Relationship Between Peripheral Inflammation And Alzheimer’S Disease, Davis Cammann

UNLV Theses, Dissertations, Professional Papers, and Capstones

Alzheimer’s disease (AD) is the leading cause of dementia worldwide, and has become an ever-present problem in aging populations. An increasing body of evidence suggests that neuroinflammation is one of the key drivers of AD pathology. One overlooked contributor to this burden is peripheral inflammation throughout the body. Due to increased permeability of the blood-brain-barrier (BBB) in older age, inflammatory plasma proteins and immune cells infiltrate the CNS and drive neuroinflammation through interactions with neurons and glia. In addition, age-related changes in the composition of gut microbiome taxa lead to increased gut permeability and inflammatory burden. Because inflammatory factors are …

The Influence Of Concussion History And Head Acceleration On Lower Extremity Function, Warren Forbes

UNLV Theses, Dissertations, Professional Papers, and Capstones

Sport-related concussions (SRCs) play a very debilitative role in athletics with between 1.7 – 3 million SRCs occurring annually (Clay et al., 2013; Zuckerman et al., 2015). This equates to upwards of 21.5 SRCs per 1000 athletic exposures. Research pertaining to SRC has received intense focus because of the substantial financial impact on the US healthcare system as well as the negative impact that SRCs have on the quality of life of athletes. Much of the research concerning SRC has been centered around management and ensuring adequate recovery from SRC-related symptoms. The current return to play (RTP) criteria from an …

Cellular Heat Responses Of Desert Mammals Promote Survival In Extreme Conditions, Janessa Montenegro

UNLV Theses, Dissertations, Professional Papers, and Capstones

Increasing temperatures with the potential for heat stress is among the future threats facing many mammals. Cellular mechanisms to maintain homeostasis in the face of heat stress likely differ across species based on naturally tolerated intracellular variability. In some mammals, the homeostatic set point in cells has a narrow range. Other species tolerate more varied intracellular conditions, including in temperature. Heat stress responses of cultured dermal fibroblasts from several mammals were compared to detect cell proliferation and cellular death (chapter 2). Cells from heat- tolerant species (13-lined ground squirrel, dromedary camel, round-tailed ground squirrel, and white-tailed antelope squirrel) were compared …

Mob Family Proteins And Tricornered Kinase Are Required To Form Dorsal Appendages Of The Drosophila Eggshell, Keala H. D. Watson

UNLV Theses, Dissertations, Professional Papers, and Capstones

To understand how complex tissues are formed, we need to understand how cells communicate with other cells to build structures. In Drosophila melanogaster, epithelial cells form tubular structures to shape the dorsal appendages on the eggshells. One signaling pathway needed for this event is the Hippo-like kinase intracellular pathway. I focus on two key components in this pathway: the NDR kinase Tricornered (Trc) and Mob proteins. In eukaryotes, Mob proteins are known to interact with NDR kinases to promote their activation. The Hippo- like pathway involves the NDR kinase Trc; however, the specific Mob protein(s) that interact with Trc …

Western Pearlshell (Margaritifera Falcata) Extirpation In The Smith River, Montana, With A Possible Link To Warming Water Temperatures, David M. Stagliano

Western North American Naturalist

Western pearlshell (Margaritifera falcata) populations are in decline across the species’ range in western North America. In 2008, Montana categorized the western pearlshell as an imperiled species of concern (S2) based on reduced detections at historically occupied sites and few viable populations. Between 2004 and 2014, we surveyed 24 Smith River reaches (61 total sites in the watershed) for the presence of western pearlshell populations. Despite historical and anecdotal descriptions of abundant western pearlshell in the Smith River, intensive structured surveys between 2004 and 2007 documented only 4 sections with low-density, nonviable western pearlshell populations (<10 individuals, large size classes [average total length = 70 mm], with no signs of recruitment). In 2014, we revisited these occupied sites to repeat surveys (expending significantly more search time) and observed no live individuals. This was surprising given that, although those populations would certainly die out without recruitment, older individuals can oftentimes persist for decades. Therefore, we consider the western pearlshell mussel extirpated from the Smith River watershed. We reviewed long-term USGS gage discharge and temperature data near the western pearlshell populations to evaluate the potential causal factors for, and timing of, the extirpation. From 3 July to 3 August 2007, water temperatures were at or above the thermal stress levels of the western pearlshell (>25 °C, …

Consumption Of A Western Diet Enhanced Colitis-Associated Colorectal Cancer And Dysbiosis Of The Fecal Microbiome In Mice Notwithstanding Dietary Intervention Or Fecal Microbiome Transfer, Daphne Michelle Rodriguez Jimenez

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

In a rodent model of inflammation-associated colorectal cancer, consumption of a Western-style diet increases gut inflammation and enhances risk of developing colon tumors. The goal of this dissertation was to understand the contribution of bacteria within the large intestine on colon inflammation and colon tumorigenesis. Two pre-clinical animal studies were performed using two different intervention strategies to shift the microbiome, and potentially gut inflammation and tumor development: 1) an experiment using dietary supplementation with black raspberries, a functional food enriched in bioactive anthocyanins with purported antiinflammatory activity, and 2) an experiment using fecal microbiota transfer from mice fed a healthy …

Investigating Mitochondrial Influence On The Rate Of Anaerobic Glycolysis In An In Vitro Model, Mackenzie Jenna' Taylor

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The rate at which the muscle acidifies after an animal is harvested has a profound effect on the quality of the resulting pork. When acidification increases gradually, desirable pork quality characteristics are developed. In contrast, rapid acidification deteriorates pork quality, exemplified by the pale, soft, and exudative (PSE) pork defect. The rate of acidification is determined by the rate of anaerobic metabolism in postmortem muscle. Yet the processes controlling postmortem anaerobic metabolism are not well understood. Recent research suggests that mitochondria, the powerhouse of the cell, may influence this process by competing for substrate (pyruvate) with anaerobic metabolism, thereby reducing …

Water Conservation Through Drought-Resilient Landscape Plants And Deficit Irrigation, Ji-Jhong Chen

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Increases in urban population and inadequate rainfall result in imbalanced water budgets in urban and sub-urban regions. Water conservation becomes important in urban landscapes because of increased water demands. Modern landscape designs require drought-resistant plants to maintain urban landscape greenness during water scarcity. Irrigating plants at their irrigation requirements, which is the minimum irrigation rate that can achieve acceptable aesthetic quality, can also conserve water. However, the drought tolerance and irrigation requrements of most landscape plants have not been widely evaluated. Shepherdia ×utahensis ‘Torrey’ (‘Torrey’ buffaloberry) and Penstemon species (beardtongues) are low-water-use landscape plants, but their drought resistance mechanisms are …

Multispecies Genomic Sex Identification Using Ddx3 Gene Polymorphisms, Jessica Felts

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

PCR sex determination assays must be reliable and cost effective due to the frequent and integral use of these assays in biological research and the animal production industry. Thus, the design of proof of a primer pair with a built-in control is warranted to not only bypass the extra cost of a multiplex reaction, but also to prevent anomalous results that have been documented with other primer pairs.

The objective of this study was to design primer pairs with built in PCR amplification control to identify sex in Equus caballus (domestic horse), Homo sapiens (humans), Macaca mulatta (rhesus macaque), and …

Precipitation And Soil Properties Determine Long-Term Consequences Of Disturbance And Invasion In Drylands, Tyson Terry

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Disturbance and invasive species have dramatic effects on desert plant communities, often resulting in degradation or shifts to alternative plant communities. Climate and soil properties determine water availability to plants, and have been thought to drive patterns of recovery following disturbance and potential for invasion.

In chapter II we used a combination of natural gas pipelines and satellite imagery to understand how recovery from a uniform disturbance differs across precipitation and soil gradients. We used a recovery ratio (disturbed/undisturbed) of pipeline pixels and their undisturbed nearest neighbor pixel to quantify recovery in a comparable way across precipitation gradients. We found …

Opening The Black Box: Soil Microbial Communities In Field-Based Plant-Soil Feedback Experiments, Julia Kate Aaronson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Plant-soil feedback is a process through which plants modify the properties of their associated soils, affecting their growth. PSF can play a key role in regulating plant growth and communities including altering plant invasion, rarity, and abundance. However, our understanding of the soil organisms that drive these plant growth responses is limited. Most studies treat soils as a ‘black box’ and do little to reveal which specific microbes or microbial communities may be responsible. This chapter examines two recent large PSF field experiments conducted in Minnesota, USA, and Jena, Germany. These experiments revealed that plants altered their soils, changing subsequent …

A Statewide Evaluation Of Fuel Treatment Effectiveness In Altering Wildfire Outcomes On Public Lands In Utah, Jamela Charmaine Thompson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Fuel treatments are land management activities that reduce living and dead flammable materials on the landscape to mitigate undesirable wildfire behavior and effects. Common treatments in the western United States include mechanical methods such as thinning and mastication, prescribed burns, and chemical methods, such as herbicide application. Treatments usually have multiple objectives, including reducing fire intensity, protecting natural and cultural resources, slowing or disrupting a potential future fire’s path, supporting ecosystem health, and reestablishing low to mid severity fire cycles in ecosystems. Although treatments can potentially modify fire behavior and ecological health, they generally cannot prevent fires from igniting, eliminate …

A Mechanistic Examination Of Interspecific Competition Between Wild And Domestic Herbivores, Courtney Check

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Large herbivores, such as mule deer and cattle have similar life histories and likely compete for resources. However, quantifying the extent to which these species compete and the specific resources they compete for has proved challenging. My research examines if cattle influence deer abundance and behavior due to competition for forage, competition for shade, and/or by affecting the predation risk of deer. Using a grid of autonomous trail cameras, I was able to determine if cattle abundance influences local deer abundance in relation to specific resources and habitat features. Using GPS data from collared deer, I was also able to …

Greater Sage-Grouse Response To Tree Canopy Removal: Habitat Vegetation Composition And Sage-Grouse Use 10–15 Years Post Treatment In The Southern Periphery Of The Species Range, Benjamen Donnelly

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Pinyon juniper woodland expansion into Greater sage-grouse (Centrocercus urophasianus, hereafter ‘sage-grouse’) habitat in southern Utah continues to threaten sage-grouse survival. Habitat restoration after pinyo-juniper removal treatment is key to the continued persistence and survival of sage-grouse along the southern edge of their range. Few long-term studies are available that examine sage-grouse use of areas treated to remove pinyon-juniper forests (i.e., ‘restored habitat’). The purpose of this research was to determine if sage-grouse used ‘restored’ areas in the long-term, as a measure of the actual effectiveness of pinyon-juniper woodland control treatments. I compared vegetation composition, shrub height and shrub …

Evaluating Beaver Translocation Methods For Desert River Restoration, Christine E. Sandbach

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Wildlife translocation, or moving wild animals from their original home to a new location, is a common conservation practice; however, translocation programs have variable success rates. Beaver translocation is often used in stream restoration projects due to beavers’ role as ecosystem engineers—beavers enhance riparian habitat by building dams that hold water and create more diverse channels. Beaver translocation success is often limited by high mortality and long distance movement after release, and improvement in translocation methods is needed. My objective was to evaluate two methods of improving beaver translocation success in a degraded desert river in east-central Utah: beaver dam …

From Flames To Forage: How Wildfire Affects Elk Behavior And Abundance, Megan M. Whetzel

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The Rocky Mountain elk (Cervus elaphus nelsoni) is an ecologically and culturally important wildlife species in the Intermountain West, but it is facing habitat changes caused by increasing fire activity. Wildfire frequency is projected to continue to change into the future, yet increases in annual area burned and increases in area burned at high severity may actually represent opportunities for some species. Large herbivores like elk may benefit from increased access to regenerating areas where forage abundance and quality are often elevated. Therefore, effective management of wildlife populations may depend on quantifying how large ungulates, like elk, alter …

Happy Or Sad? Quaking Aspen (Populus Tremuloides Michx.) On Cedar Mountain, Southern Utah, Jaycee Cappaert

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Quaking aspen forests are important communities in the western United States where they provide preferential food and habitat for animals, limit wildfires from spreading, and have favorable aesthetics, among other things. Their putative decline is an issue for ecology and management. We remeasured the aspen population on Cedar Mountain, Utah, to assess changes to its condition since it was originally surveyed in 2008. We found that the area comprised of stable aspen (>80% aspen) decreased from 84.3% to 69.7%. The amount of mortality has increased slightly over the last decade, but crown dieback has stayed virtually identical. Additionally, the …

An Evaluation Of Stress Tolerance In Restoration Plant Species In Response To Fire, Drought, And Invasive Plants Through The Lens Of Functional Traits, Adam M. Clifford

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Sagebrush communities in the Great Basin face many threats. Cheatgrass, a winter annual from Eurasia, has invaded these communities, increasing wildfire and lowering species diversity. Additionally, climate models project warmer and drier conditions throughout much of the Great Basin, likely increasing drought, cheatgrass invasion, and wildfire. Intact stands of native and introduced perennial grasses have been shown to limit invasion by cheatgrass and restore ecosystem functions. The objective of this research was to identify the functional traits and growth characteristics needed by restoration species to survive periods of drought and to evaluate varieties of commonly used restoration species for establishment, …

Population Physiology, Demography, And Genetics Of Side-Blotched Lizards (Uta Stansburiana) Residing In Urban And Natural Environments, Spencer B. Hudson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Wildlife populations across the globe are poised to lose their natural habitat to urbanization, yet there is limited information on how different species handle living in cities. Animals in urban environments are often susceptible to novel stressors, which can threaten their individual health and population viability. The physiological characteristics of animals, such as those related to metabolic hormones, oxidative stress, and immunity, are expected to be important for survival in this context. If so, animals persisting in urban areas may demonstrate physiological differences from their natural counterparts, perhaps due to evolutionary change. These potential outcomes have been documented in birds …

Mountain Lion (Puma Concolor) And Feral Horse (Equus Ferus) Interactions: Examining The Influence Of A Non-Native Ungulate On Predator Behavior In A Semi-Arid Environment, Peter C. Iacono

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

A non-native is a species that evolved in one ecosystem and has established within another. Non-native species can thrive in new ecosystems as they can spread quickly, outcompete and replace native species, and disrupt food webs. Domestic horses were brought to North America by Europeans in 1493. They are now found in 11 states across the western United States. Feral horses can negatively impact vegetation, endangered species habitat, compete with native species, and be important prey for mountain lions under certain conditions. But do feral horses affect the food web? Feral horses are managed by large removals to reduce the …

Overcoming Barriers To Aquatic Plant Restoration: Addressing Gaps In Species Identification And Planting Techniques In The Intermountain West, Kate A. Sinnott

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Aquatic ecosystems provide many critical and economically valuable benefits, including drinking water, food, recreational opportunities, and water supply for irrigation and agriculture. However, the health of these systems has been severely impacted by human activities such as pollution, land conversion, and introductions of harmful species. Restoring native aquatic plants can help reverse this damage and reestablish benefits, though it is not a common practice. With an objective to increase capacity for aquatic plant restoration in the Intermountain West, I identified and addressed two major barriers: 1) a lack of confidence in aquatic species identification among wetland professionals, and 2) underdeveloped …

Impact Of Forage Conditioner Systems On The Harvesting Of Alfalfa, Derrick Hendry

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This study analyzed three major brands of conditioner rollers and determined how they performed in drying the forage crop alfalfa. Each type of conditioner roller would cut a wide and narrow windrow. All the windrows were tested and analyzed the same. The study was concluded once the forage was baled.

Treatment Of Yellow Fever Virus With The Ns4b Inhibitor Bdaa And Effects On Rna‐Sensing Innate Immune Pathways In Hamster And Mouse Models, Abbie E. Weight

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Yellow fever (YF) is an acute and often severe disease cause by yellow fever virus (YFV). Although there is an effective vaccine available to prevent YF, there are no antiviral drugs approved to treat the disease, which has a considerable disease burden in endemic areas of South America and Africa. BDAA is an experimental antiviral treatment which has shown efficacy against YFV both in cell culture and when administered before infection in an animal model of disease. BDAA targets the YFV protein NS4B and has two reported mechanisms of action: the primary mechanism of action is the direct inhibition of …

Developing A Predictive Geospatial Habitat Model For A Rare Species Of Salamander In Tennessee: A Case Study For The Green Salamander (Aneides Aeneus), Erin Gaylord

Masters Theses and Doctoral Dissertations

Green Salamanders (Aneides aeneus; Cope and Packard 1881) are a secretive and cryptic species of salamander that can be relatively difficult to detect, due to their occupation of arboreal habitats. The incorporation of geospatial tools is critical in developing models that can be used to predict undocumented locations in Tennessee and elsewhere. Locating the species in Tennessee was accomplished through a total of 18 localities and with that, several habitat characteristics were able to be identified including, but not limited to: appropriately shaded and structured rocks, typical fauna co-occurring within the area, and general topography of the area. Of the …

Hair And Drugs: The Impact Of Dry Shampoo And Gel On Cocaine-Contaminated Hair, Marialejandra Faure Betancourt

Student Theses

Hair is a biological sample used in forensic toxicology to identify and quantify drug exposure. However, when interpreting analytical results, hair can be challenging due to the presence of external contamination. Previous studies have shown that cosmetic treatments remove drugs within the hair shaft decreasing their concentration. Nevertheless, not enough information is available to explain this phenomenon. This study focused to determine whether cosmetic products such as dry shampoo and gel influence the concentration of externally deposited cocaine (COC) and benzoylecgonine (BE) in different hair types/colors. Hair samples from fifteen COC-free volunteers of various hair colors/types were collected and divided …