Life Sciences Commons^™

Computational Analysis Of Antibody Binding Mechanisms To The Omicron Rbd Of Sars-Cov-2 Spike Protein: Identification Of Epitopes And Hotspots For Developing Effective Therapeutic Strategies, Mohammed Alshahrani

Computational and Data Sciences (PhD) Dissertations

The advent of the Omicron strain of SARS-CoV-2 has elicited apprehension regarding its potential influence on the effectiveness of current vaccines and antibody treatments. The present investigation involved the implementation of mutational scanning analyses to examine the impact of Omicron mutations on the binding affinity of four categories of antibodies that target the Omicron receptor binding domain (RBD) of the Spike protein. The study demonstrates that the Omicron variant harbors 23 unique mutations across the RBD regions I, II, III, and IV. Of these mutations, seven are shared between RBD regions I and II, while three are shared among RBD …

Trophic Assessment Of The Invasive Hydrozoan Gonionemus Vertens Using Stable Isotope Analysis : You Are What You Eat, Diana Sisk-Gritz

Theses, Dissertations and Culminating Projects

Gonionemus vertens, commonly known as the clinging jellyfish, is a small hydrozoan known for their conflicts with humans due to their neurotoxins that can lead to paralysis. Native to the Pacific Ocean, it has now invaded numerous ecosystems in the coastal waters of the Mediterranean Sea, Europe, and the United States. In the United States, it has flourishing populations from New Jersey to Maine. However, many questions remain as to how it arrived and spread, but equally important is what impact this species may have in the invaded communities they now reside. Research suggests that G. vertens actively feeds on …

Assessing The General Public's View Of Direct-To-Consumer (Dtc) Genetic Testing And Their Interpretation Of Dtc Website Disclaimer Messages., Madison Ruehl, Shelly Hovick, Alisdair Philp, Kevin Sweet

Medical Education Articles

The general public continues to show increased interest and uptake of Direct-to-Consumer (DTC) genetic testing. We conducted an online survey (N = 405) to assess genetics knowledge, interest, and outcome expectancy of DTC genetic testing before and after exposure to a sample DTC disclaimer message. Descriptive statistics were used to analyze the relationship between previous genetic knowledge, attitudes and self-reported systematic processing of a sample DTC disclaimer message, outcome expectancies, and interest to pursue DTC genetic testing. Increased genetic knowledge and more positive attitudes towards DTC genetic testing were associated with increased self-reported systematic processing of the DTC disclaimer message. …

Investigation Of Orthohantavirus Genetics In Rodent Reservoirs And Clinical Samples, Samuel Melvin Goodfellow

Biomedical Sciences ETDs

Orthohantaviruses are negative-sense, single stranded RNA viruses with trisegmented genomes that can cause severe disease in humans and are carried by several host reservoirs throughout the world. In the United States, Sin Nombre orthohantavirus (SNV) is the primary cause of hantavirus cardiopulmonary syndrome (HCPS) with a fatality rate of ~36% and the highest cases occuring in the southwest region. The primary SNV host reservoir is thought to be the western deer mouse, Peromyscus maniculatus, however it has been shown that other rodents can carry different orthohantaviruses. We designed a pan-orthohantavirus detection tool to survey several small mammal populations throughout New …

Proceedings Of The 2023 Berry Summer Thesis Institute, University Of Dayton. University Honors Program

Proceedings of the Berry Summer Thesis Institute (2013-Present)

Thanks to a gift from the Berry Family Foundation and the Berry family, the University Honors Program launched the Berry Summer Thesis Institute in 2012. The institute introduces students in the University Honors Program to intensive research, scholarship opportunities and professional development. Each student pursues a 12-week summer thesis research project under the guidance of a UD faculty mentor. This contains the product of the students' research.

Contents:

• “How Porous Materials Affect the Boundary-Layer Transition of Hypersonic Flight Vehicles” (Megan C. Sieve)
• “Ultra-Stretchable, Self-Healing, DLP 3D-Printed Elastomers for Damage-Resistant Soft Robots: A Review” (Robert M. A. Drexler)
• “Extrapolation of Scalar …

How Does Increasing The Darkness Of A Plate Affect The Perception Of Both Bitter And Sweet Flavors?, Jessica Williams

Food Science Undergraduate Honors Theses

Extrinsic cues like plate color have been shown to effect taste perception. Previous studies conducted show a consensus that plate colors can modulate taste perception (Piqueras-Fizman et al., 2012, Stewart & Gross, 2013). No studies, however, have investigated varying color hues and their effect on taste perception. This study aims to determine whether the change in the black to white ratio of plate color can affect consumer perception of sweetness or bitterness.

We presented a group of volunteers two different brownies on plates varying from 100% white to 0% white in 25% increments. A 30:70 male to female gender ratio …

Sex Differences In Resting Metabolic Rate Among Athletes And Association With Body Composition Parameters: A Follow-Up Investigation, Andrew R. Jagim, Margaret T. Jones, Andrew T. Askow, Joel Luedke, Jacob L. Erickson, Jennifer B. Fields, Chad Kerksick

Faculty Scholarship

The purpose of this study was to examine sex differences in resting metabolic rate (RMR) and associations between measured RMR and body composition parameters in athletes. One-hundred and ninety collegiate men (n = 98; age: 20.1 ± 1.6 yr.; body mass: 92.7 ± 17.5 kg; height: 181.6 ± 6.2 cm, body mass index: 28.0 ± 4.7 kg/m2) and women (n = 92; age: 19.4 ± 1.1 yr.; body mass: 65.2 ± 11.0 kg; height: 168.0 ± 6.6 cm, body mass index: 23.0 ± 3.6 kg/m2) athletes volunteered to participate in this study. Athletes completed a body composition assessment using air …

A Randomized Controlled Trial To Examine The Impact Of A Multi-Strain Probiotic On Self-Reported Indicators Of Depression, Anxiety, Mood, And Associated Biomarkers, Kylie E. Walden, Jessica M. Moon, Anthony M. Hagele, Leah E. Allen, Connor J. Gaige, Joesi M. Krieger, Ralf Jäger, Petey W. Mumford, Marco Pane, Chad M. Kerksick

Faculty Scholarship

Objective: To examine the efficacy of supplementing with a multi-strain probiotic (MSP) on changes associated with mood, anxiety, and neurotransmitter levels.

Method: In a randomized, double-blind, placebo-controlled fashion, 70 healthy men and women (31.0  ±  9.5  years, 173.0  ±  10.4  cm, 73.9  ±  13.8  kg, 24.6  ±  3.5  kg/m2 ) supplemented with a single capsule of MSP (a total daily dose of 4 × 109 colony forming units [CFU] comprised of a 1 × 109   CFU dose from each of the following strains: Limosilactobacillus fermentum LF16, Lacticaseibacillus rhamnosus LR06, Lactiplantibacillus plantarum LP01, and Bifidobacterium longum 04, Probiotical S.p.A., Novara, …

Quantifying The Impacts And Assessing The Permeability Of A Divided Four-Lane Highway On Migratory Mule Deer, Daniel P. Taylor

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Increased human development and expansion has led to an ever-growing transportation network, which has both direct and indirect ecological impacts to local wildlife populations. In response, wildlife crossing structures have become an increasingly fundamental component of wildlife conservation throughout the United States. The focus of this study was a 13.5 km section of US Highway 395, Mono County, California. The California Department of Transportation recently identified this section of highway, hereafter known as the Mammoth Wildlife Crossing, as the highest priority location for wildlife crossing structures along US 395 within Mono County. The goal of this study was to use …

Reduced Growth Mindset As A Mechanism Linking Childhood Trauma With Academic Performance And Internalizing Psychopathology, Lucy A. Lurie, Emily J. Hangen, Maya L. Rosen, Robert Crosnoe, Katie A. Mclaughlin

Neuroscience: Faculty Publications

Background

Despite the high prevalence of childhood adversity and well-documented associations with poor academic achievement and psychopathology, effective, scalable interventions remain largely unavailable. Existing interventions targeting growth mindset—the belief that personal characteristics are malleable—have been shown to improve academic achievement and symptoms of psychopathology in youth.

Objective

The present study examines growth mindset as a potential modifiable mechanism underlying the associations of two dimensions of childhood adversity—threat and deprivation—with academic achievement and internalizing psychopathology. Participants and setting Participants were 408 youth aged 10–18 years drawn from one timepoint of two longitudinal studies of community-based samples recruited to have diverse experiences …

Water Conservation Through Drought-Resilient Landscape Plants And Deficit Irrigation, Ji-Jhong Chen

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Increases in urban population and inadequate rainfall result in imbalanced water budgets in urban and sub-urban regions. Water conservation becomes important in urban landscapes because of increased water demands. Modern landscape designs require drought-resistant plants to maintain urban landscape greenness during water scarcity. Irrigating plants at their irrigation requirements, which is the minimum irrigation rate that can achieve acceptable aesthetic quality, can also conserve water. However, the drought tolerance and irrigation requrements of most landscape plants have not been widely evaluated. Shepherdia ×utahensis ‘Torrey’ (‘Torrey’ buffaloberry) and Penstemon species (beardtongues) are low-water-use landscape plants, but their drought resistance mechanisms are …

A Comparison Of Pm-Nato3’S Influence On Neural Progenitors And Mature Dopamine Neurons, Mary E. West

Masters Theses

This thesis presents significant findings regarding the role of PM-Nato3 in its interaction with developing neurons in the context of Parkinson's disease (PD) and regenerative medicine. We investigated the effects of PM-Nato3 on dopamine (DA) neurogenesis under different culture conditions, both in vitro and in vivo. In the standard dopaminergic culture condition, PM-Nato3 potentially increased the speed of DA neuron production but did not significantly increase the yield of DA neurons. In a minimal culture condition, there was no notable difference between the control and PM-Nato3 conditions, suggesting minimal impact on DA neurogenesis. In vivo studies using a mouse model …

Recreational Fishing For Western Rock Lobster: Estimates Of Participation, Effort And Catch In 2022/23, Claire B. Smallwood, Karina L. Ryan, Alissa C. Tate, Lucy J. Rudd

Fisheries Research Reports

The Western Rock Lobster (WRL) (Panulirus cygnus) is the most commonly caught Rock Lobster (RL) species in Western Australia. Catches from the commercial and recreational sectors are required to determine and monitor Total Allowable Commercial Catch (TACC), Total Allowable Recreational Catch (TARC) and proportion of Allowable Harvest Level (AHL) achieved. The TARC is set at 5% of the AHL and evaluated in the harvest strategy based on a 5-year average. Data for the recreational sector are obtained using phone-recall surveys to provide estimates of participation, fishing effort and retained catch (by numbers) from recreational fishers who hold a RL licence. …

The Effect Of Entrepreneurial Traits On Career Planning In Food Processing Enterprises, Ali S. Cetinkaya

University of South Florida (USF) M3 Publishing

Economic, social, and technological changes create new demands and opportunities for people's jobs. Entrepreneurship is one way to deal with these demands and take advantage of new opportunities. Career planning assists an individual in becoming aware of various career options, including entrepreneurial intent. Even if they have all of the necessary resources, not everyone wants to start a new business. Some people have specific entrepreneurial characteristics that motivate them to pursue a career investing in new businesses. The purpose of this study was to determine the impact of individuals' entrepreneurial traits on their career planning. The survey technique was used …

Examining Potential Trade-Offs Between Pest Management And Pollination To Sunflower Production In Nebraska, Luis Ochoa Cadena

Department of Entomology: Dissertations, Theses, and Student Research

Sunflower (Helianthus annuus) is cultivated for oil and seed production worldwide, and the United States produces 2.5% of the world’s oilseed sunflower primarily grown in the Great Plains. In 2022, oilseed and confectionary U.S. sunflower production increased by 48 and 44%, respectively, compared to last year's records. However, sunflower production can be seriously compromised by insect pests. The red sunflower seed weevil (Smicronyx fulvus LeConte) is considered the most serious pests in North America and causes severe economic damage yearly. On the other hand, native bees contribute substantially to sunflower yield. Currently, insecticide applications are the primary tool to suppress …

Exploring Experimental Design And Multivariate Analysis Techniques For Evaluating Community Structure Of Bacteria In Microbiome Data, Kelsey Karnik

Department of Statistics: Dissertations, Theses, and Student Research

The gut microbiome plays a crucial role in human health, and by working collaboratively with microbiologists, we aim to further our understanding of the human gut and its impact on human health. Promoting a diverse microbiome is emphasized throughout microbiology literature, and involving a statistician in designing experiments to relate gut bacteria and some measured health outcome is crucial for ensuring valid and accurate results. By adopting new experimental design and analysis methods, researchers can begin to gain a deeper understanding of how the genetics of our food affect the composition of taxa within the gut microbiome. This dissertation is …

The Effects Of Betaine Supplementation On Fluid Balance And Heat Tolerance During Passive Heat Stress In Men, Brandon D. Willingham, Liliana I. Rentería, Tristan J. Ragland, Michael J. Ormsbee

Kinesiology

Introduction: Consuming intracellular osmolytes, like betaine (BET), may attenuate symptoms of heat stress. The purpose of this study was to examine the effects of BET supplementation on fluid balance and heat tolerance after a 7-day loading period and during passive heat exposure. Methods: A double-blind, placebo controlled, crossover study compared BET or placebo consumption (50 mg·kg⁻¹, twice daily) for 7 days in young, recreationally active men (N = 11). Results: During the loading period, no significant interactions were found for any marker of fluid balance between or within conditions. During heat exposure, significant time effects but no condition …

Vegetation Survey Data For Spring Run Experimental Site, Valerie N. Thurston, Ryan W. Mcewan, Anna R. Pallone, Mary C. Lloyd

Data Files: Ecosystem Restoration Following Industrial Agriculture

This spreadsheet is the percent cover of each species surveyed in the Spring Run experimental restoration site (39° 46 '42.2"N, 84° 20' 52.5"W) collected at the quadrat level.

DOI: https://doi.org/10.26890/fdw4698

Investigating And Characterizing Septin Cellular Regulators During Infection Related Development In M. Oryzae, Rinalda Proko

Graduate Theses and Dissertations

The fungus Magnaporthe oryzae causes a devastating disease of rice and wheat, called blast, which poses a major threat to food security across the planet. To enter plant tissue, M. oryzae produces a remarkable pressure-generating infection cell called an appressorium, which mechanically ruptures the tough cuticle of host leaves through the application of enormous physical force directed onto a narrow penetration structure emerging from its base. Importantly, appressorium form and functionality depends on the regulated assembly of a toroidal ring-like structure, composed of septins, which forms within its base. Septins are a family of membrane-associated GTP-binding proteins that polymerize into …

Screening A Set Of Tomato Genotypes For Resistance To Bacterial Wilt And A Genomic Wide Association Study And Genomic Prediction For Yield-Related Traits In ‘Lcp 85-384’. I. Sugarcane, Theresa Makawa Phiri

Graduate Theses and Dissertations

This thesis comprises two studies that focused on screening tomato genotypes for bacterial wilt resistance and a genome-wide association study and genomic prediction for yield-related traits in sugarcane with the aim of contributing to the breeding programs. In the first chapter, 40 tomato accessions from the USDA collection were evaluated for their resistance to Ralstonia solanacearum isolate P822 under controlled greenhouse conditions. The plants were inoculated, and disease symptoms were visually assessed. Disease severity was rated on a scale of 0 to 4, where 0 represented no wilting and 4 indicated complete leaf wilting. Among the tested accessions, five (PI …

B. R. Wells Arkansas Rice Research Studies 2022, J. Hardke, X. Sha, N. Bateman

Arkansas Agricultural Experiment Station Research Series

Arkansas is the leading rice producer in the United States. The state represents 50.1% of total U.S. rice production and 49.8% of the total acres planted to rice in 2022. Rice cultural practices vary across the state and across the U.S. However, these practices are also dynamic and continue to evolve in response to changing political, environmental, and economic times. This survey was initiated in 2002 to monitor and record changes in the way Arkansas rice producers approach their livelihood. The survey was conducted by polling county extension agents in each of the counties in Arkansas that produce rice. Questions …

Studying The Stability Of Collagen/Heparin Coatings To Be Used In Cell Therapy Applications, Gavin Mussino

Biological Sciences Undergraduate Honors Theses

This honors thesis aims to investigate the reusability and performance of cell coatings for cell therapy applications. Cell therapy, which involves the use of human cells to repair or replace damaged tissues, holds immense potential for medical advancements. However, ensuring the survival and functionality of transplanted cells remains a significant challenge. We focused on studying the effectiveness of coatings applied to cells for improved cell growth and viability. The research project involved the preparation of the coatings using a layer-by-layer method and the subsequent seeding of cells. The coated cells were then subjected to a series of experiments to assess …

Anything But Bland: Uncovering The Hidden Diversity And Genomic Origin Of The Threatened Vanilla Spice, Paige Ellestad

Boise State University Theses and Dissertations

Conserving the genetic diversity of crop species and their wild relatives has become a mounting concern as the detrimental effects of climate change, habitat destruction, and genetic erosion are being realized. In this epoch of unprecedented biodiversity loss, the genetic resources needed to improve crops may be at risk of extinction. Even one of the most iconic spices, vanilla, is threatened. Wild populations of the main vanilla producing species, Vanilla planifolia Andrews (Orchidaceae), are being rapidly extirpated due to deforestation and illegal harvesting in their native range. On top of that, clonal propagation methods within cultivated plants are hypothesized to …

Age-Related Differences In Kinematics, Kinetics, And Muscle Synergy Patterns Following A Sudden Gait Perturbation: Changes In Movement Strategies And Implications For Fall Prevention Rehabilitation, Woohyoung Jeon, Ahmed Ramadan, Jill Whitall, Nesreen Alissa, Kelly Westlake

Health & Kinesiology Faculty Publications and Presentations

Falls in older adults are leading causes of fatal and non-fatal injuries, negatively impacting quality of life among those in this demographic. Most elderly falls occur due to unrecoverable limb collapse during balance control in the single-limb support (SLS) phase. To understand why older adults are more susceptible to falls than younger adults, we investigated age-related differences in lower limb kinematics, kinetics, and muscle synergy patterns during SLS, as well as their relationship to postural control strategies. Thirteen older and thirteen younger healthy adults were compared during the SLS phase of balance recovery following an unexpected surface drop perturbation. Compared …

Visual Circuitry In Adaptive Behavior: Extraction Of Complex Visual Image Information By Arrays Of Non-Image Forming Eyes, Thomas Kipp Halladay Groves

Dissertations

A complete understanding of how neural circuitry underlies the detection and integration of visual cues continues to elude researchers in the 21st century. Since the dawn of life on earth, light has exerted enormous selective pressure on all living organisms. Visual systems represent one of the most common sensory systems in existence, with significant homologies among elements of these systems, such as photopigments and developmental genes. Offering nearly unmatched single cell access at all levels of its nervous system, the European medicinal leech (Hirudo spp.) is an excellent system in which to identify behaviorally relevant neuronal circuits. Hirudo evolved discrete …

Integrating Sensor Development, Risk Assessment, And Community Engagement To Support Environmental Justice In The Rural Community Of El Tiple, Colombia, David Bahamon Pinzon

All Dissertations

In Colombia, ethnic communities have traditionally been responsible stewards of natural resources. They recognize the importance of these resources for their livelihood, as well as their ancestral and cultural heritage. El Tiple, a rural Afro-Colombian community, has been affected by the incursion of private corporations that promoted the expansion of sugarcane monocrops in their territory. Since the introduction of the monoculture industry, local freshwater sources have been depleted due to intensive water use for irrigation of the sugarcane crops. Additionally, the intensive usage of agrochemicals has been linked with loss of native flora, damages to family farms, and pollution of …

Mate Choice And Sexual Conflict In A Livebearing Fish, Julia C. Kasper

Master's Theses

Eavesdropping occurs when a receiver extracts information from an interaction without directly engaging with the signaler. Eavesdropping has been shown to be an effective way of evaluating the quality of potential mates and their abilities in male-male competition, without having to directly interact with them, thereby reducing energy costs and mating harassment. Girardinus metallicus is a livebearing poeciliid fish endemic to Cuba whose mating system is dominated by mating harassment in the form of sneak copulations, persistent displaying, and male-male aggression. G. metallicus has a male specific polymorphism in both melanin coloration and behavior. Males with melanin coloration are known …

Fructose Induced Khk-C Can Increase Er Stress Independent Of Its Effect On Lipogenesis To Drive Liver Disease In Diet-Induced And Genetic Models Of Nafld, Se-Hyung Park, Robert N. Helsley, Taghreed Fadhul, Jennifer L. S. Willoughby, Leila Noetzli, Ho-Chou Tu, Marie H. Solheim, Shiho Fujisaka, Hui Pan, Jonathan M. Dreyfuss, Joanna Bons, Jacob Rose, Christina D. King, Birgit Schilling, Aldons J. Lusis, Calvin Pan, Manoj Gupta, Rohit N. Kulkarni, Kevin Fitzgerald, Philip A. Kern, Senad Divanovic, C. Ronald Kahn, Samir Softic

Saha Cardiovascular Research Center Faculty Publications

Non-alcoholic fatty liver disease (NAFLD) is a liver manifestation of metabolic syndrome, and is estimated to affect one billion individuals worldwide. An increased intake of a high-fat diet (HFD) and sugar-sweetened beverages are risk-factors for NAFLD development, but how their combined intake promotes progression to a more severe form of liver injury is unknown. Here we show that fructose metabolism via ketohexokinase (KHK) C isoform leads to unresolved endoplasmic reticulum (ER) stress when coupled with a HFD intake. Conversely, a liver-specific knockdown of KHK in mice consuming fructose on a HFD is adequate to improve the NAFLD activity score and …

Functional Analysis Of Two Campoletis Sonorensis Ichnovirus Vinnexins In Drosophila Melanogaster, Peng Zhang

All Dissertations

Campoletis sonorensis Ichnovirus (CsIV) is produced by the ichneumonid parasitoid Campoletis sonorensis (the primary host). Female parasitoid wasps restrictedly replicate viruses in their ovaries and inject them into a larval lepidopteran (secondary host) during oviposition. This symbiotic virus of C. sonorensis is essential for successful parasitization. CsIV is characterized by having a large, segmented double-stranded DNA genome with a large number of genes, many of which are associated with one of five multigene families. The CsIV vinnexin gene family is one of them and has four members, including CsIV-vnxD and CsIV-vnxG. Vinnexins are homologues of insect Innexins, which form gap …

Lunchbox Hydroponics, Stacy A. Adams

Department of Agronomy and Horticulture: Faculty Publications

Growing plants with hydroponics is less complicated than it may appear when the fundamentals of the system are understood. This publication provides instruction on the construction of a simple hydroponic prototype suitable for school or youth education programs. The fundamentals of hydroponics, basic design of a solution system, seed starting, creating a nutrient solution, and plant care are explored through this engaging project. This prototype can successfully be used to grow small edible and herb plants in the classroom or at home.