Life Sciences Commons^™

Fauna As Fieldworkers: An Analysis On The Use Of Animals As A Form Of Organic Weed And Pest Management In Modern Viticulture, Mariana Del Carmen Campano

Independent Study Project (ISP) Collection

Recent data on the current state of pesticide use in global agriculture suggests serious concerns over significant overuse and negative effects on both environmental and human health, and current trends only suggest continued increases in future global pesticide use. Without prompt, large-scale intervention, this global abuse of chemical pesticides spells significant threats of not only continued harm to global health and environmental quality, but also to the long term viability of agricultural lands and the growing threat of pesticide resistant insects and weeds. Various methods have been explored in the realm of integrated pest management (IPM), but a current lack …

Intelligence Versus Inferno: How Artificial Intelligence Can Be Used To Monitor And Manage Wildfires In Europe, Maxwell Feldman

Independent Study Project (ISP) Collection

Escalating wildfire occurrences in Europe, particularly in the southern Mediterranean region, are presenting significant challenges to socioeconomic, environmental, and ecosystem services. The increasing frequency and severity of these wildfires are straining resources and emphasizing the need for a better understanding of the relationship between suppression capacity and fire behavior in wildfire management. The following research addresses the urgent need for more proactive, knowledge-based, and technologically driven fire management strategies, throughout all four stages of wildfire response – fuel and land management, fire preparedness, fire suppression, and post-fire management. Artificial Intelligence (AI) is becoming increasingly significant in climate change adaptation, especially …

Analyzing Human - Nonhuman Primate Conflict Mitigation Techniques In Mto Wa Mbu, Northern Tanzania, Lily Adams

Independent Study Project (ISP) Collection

Human – non-human primate conflict is particularly crucial due to primates’ high capacity to live among human populations. The study to analyze Human - Nonhuman Primate Conflict Mitigation Techniques, was carried in November 2023 at Mto wa Mbu, Northern Tanzania. To analyze techniques currently being used to mitigate human – non-human primate conflict, this study collected data through semi-structured interviews. Over 87% (n=35) of respondents used multiple mitigation techniques simultaneously. 80% of respondents (n=32) reported using projectiles to ward off foraging primates, 75% of respondents (n=30) reported using loud noises (made either by the voice/body or by manipulating noisemakers), 67.5% …

Representations And Realities: Misali Island's Biodiversity In Hybrid Narratives, Justin Ross Whitney

Independent Study Project (ISP) Collection

Marine Protected Areas (MPAs) are complex organisms that ebb and flow between strict and unyielding conservation doctrines often at the cost of community engagement. Misali Island is different. Conceived and advocated for by community members, this organism has navigated a tumultuous tenure of environmental degradation, periods of high and low funding, and a looming tourism presence. On top of these challenges has been a growing fishing community attempting to share in the bounty of this shockingly biodiverse setting. This study sorts through the grey space of Misali Island by applying a hybrid narrative that interfaces the marine context of the …

Lha, Lu, And Shipda: Religious Landscape In A Conservation Area, Luke Stumpfl

Independent Study Project (ISP) Collection

Living in a political, cultural, and geographic border region between Nepal and Tibet (People’s Republic of China), the families of Tsum Valley face many demands from their sociopolitical, religious, and economic climate. Practicing Tibetan Buddhism, the people uphold a set of rules implemented by the community with leadership from Serap Dorje Drukpa Rinpoche over 100 years ago that prohibits the intentional murder of all animals. Hailed by leaders and scholars as a haven of biodiversity conservation due to this customary-turned- municipal law, Tsum Valley also exists within Manaslu Conservation Area governed by an entity of the federal government of Nepal …

Analyzing Stress Levels Of Volunteers Hand-Rearing Juvenile Spectacled Flying Foxes (Pteropus Conspicillatus) At The Tolga Bat Hospital, North Queensland, Australia, Olivia Knight

Independent Study Project (ISP) Collection

This study, conducted at the Tolga Bat Hospital in North Queensland, Australia, focuses on analyzing the stress levels of volunteers hand-rearing juvenile spectacled flying foxes (Pteropus conspicillatus). Given the endangered status of this species and the intensive care required for their rehabilitation, the research aimed to understand the physical, emotional, and mental well-being of the volunteers engaged in this demanding conservation work. The study was conducted over a five-day period during the peak pupping season in November 2023, utilizing self-administered questionnaires to assess the well-being and stress levels of volunteers both before and after their nursing sessions. The participants, primarily …

Creating A Framework For An Effective Koala Recovery Center: A Case Study Of Friends Of The Koala, Nsw, Finley Scoular

Independent Study Project (ISP) Collection

Koala populations in New South Wales, Queensland, and the Australian Capital District are currently faced with factors that threaten their survival. Within one decade, their status has shifted from stable to vulnerable to endangered. The cause of this is attributed to increased exposure to dog attacks, vehicle collisions, diseases, and habitat fragmentation and destruction. The koalas' status of endangered in these territories has illuminated the need for human intervention. This study focuses on the specific form of human intervention that involves the rescue, rehabilitation, and release of wild koalas. Due to the recent koala status change from vulnerable to endangered, …

Patrimonio Cultural, Arqueología, Medio Ambiente Y Economía: ¿Cómo Ha Afectado La Construcción Del Aeropuerto Internacional De Chinchero-Cusco A Las Comunidades Y La Tierra De Chinchero?, Sydney Berenson

Independent Study Project (ISP) Collection

La urbanización gubernamental ha sido un problema para las poblaciones indígenas, el medio ambiente y la protección del patrimonio cultural durante numerosos años en todo el Perú. Más recientemente, en la pequeña ciudad de Chinchero, ubicada a unos 30 kilómetros al noroeste de Cusco, se está construyendo un nuevo aeropuerto internacional, Aeropuerto Internacional de Chinchero-Cusco (AICC), para reemplazar el actual aeropuerto nacional de Cusco. Chinchero es un importante centro urbano que está conformado por 13 comunidades campesinas que participan en la industria agrícola de la región, además consta con una importante comunidad artesanal, ruinas arqueológicas precolombinas y una gran biodiversidad. …

If We Were Fiction: Insect-Human Stories, Isabella Delaney

Independent Study Project (ISP) Collection

This creative project delves into the nonlinear, interconnected web of insect-human relations in Nepal. Through exploring humans’ perceptions and stories of insects, this work intends to remedy the limits of traditional entomological knowledge, which forgets the ubiquitous reality of insects as more than biological and ecological agents. Insects are social, economic, and ethical “characters” in our lives, and recognizing this opens up possibilities for connection, hope, and evolution in this world. The potential of the mundane, the forgettable, pulses within humans’ unique attitudes toward insects and the forces that shaped them. This potential is represented in the final product of …

Við Pollurinn: The Effects Of Human-Caused Stimuli On Harbor Seals (Phoca Vitulina) In Ísafjörður, Ella Jorgensen

Independent Study Project (ISP) Collection

Harbor seals are important for the health of Iceland’s marine ecosystems. They are normally seen hauled-out onto land to rest. There are many factors that affect the haul-out patterns of harbor seals including location, season, time of day, tide height, weather, and human-caused stimuli. The presence of human-caused stimuli can also have a negative impact on haul-out patterns and behaviors. The Icelandic harbor seal population is threatened as it has been declining since 1980. Climate change and the rise of tourism in Iceland may be a factor contributing to this decline; and there is a gap in knowledge on the …

Cannabinoid Receptor Type 1 Regulates Sequential Stages Of Migration And Morphogenesis Of Neural Crest Cells And Derivatives In Chicken And Frog Embryos, Amira Mahomed, Daljit Girn, Afrin Pattani, Brian K. Wells, Chloe C. King, Sonya Patel, Harsimran Kaur, Christina M. Noravian, Jessica Sieminski, Chi Pham, Halley Dante, Maxellende Ezin, Tamira Elul

Biology Faculty Works

The main cannabinoid receptor CB1R first shows expression during early neurula stage in chicken (Gallus gallus) embryos, and at early tailbud stage in the frog (Xenopus laevis) embryos. This raises the question of whether CB1R regulates similar or distinct processes during the embryonic development of these two species. Here, we examined whether CB1R influences the migration and morphogenesis of neural crest cells and derivatives in both chicken and frog embryos. Early neurula stage chicken embryos were exposed to arachidonyl-2ʹ-chloroethylamide (ACEA; a CB1R agonist), N-(Piperidin-1-yl)-5-(4-iodophenyl)-1-(2,4-dichlorophenyl)-4-methyl-1H-pyrazole-3-carboxamide (AM251; a CB1R inverse agonist) or Blebbistatin (nonmuscle Myosin II inhibitor) in ovo and examined during …

Defence Response Of Host Plants For Cyst Nematode: A Review On Parasitism And Defence, Rishil Gupta, Manar Fawzi Bani Mfarrej, Rehab O. Elnour, Mohamed Hashem, Faheem Ahmad

All Works

The Cyst nematodes (CN), such as Heterodera spp. and Globodera spp. are key biotrophic pathogens inflicting high levels of damage to agricultural and horticultural crops. This review sheds light on the parasitism of the CN and molecular defence responses of infected plants. We highlight the role of effector proteins secreted from the oesophageal gland cells of the CN, hormone-signalling pathway, and miRNA regulation of gene expression that modulate the differentiation of the feeding site. In addition, we speak of the role of pattern-triggered immunity (PTI), effector-triggered immunity (ETI), resistance genes (R genes), and pathogenesis-related proteins in the immune defence responses …

Computational Investigation Of Mononuclear Iron Water Oxidation Catalyst Design, Kristal Stevens, Emily Jarvis

Chemistry and Biochemistry Faculty Works

Hydrogen production from non-carbon sources is an essential component of clean and sustainable technology for reducing greenhouse gas emissions from fuels. Water oxidation, which splits water molecules into hydrogen (protons) and molecular oxygen, is a thermodynamically challenging, multistep reaction achieved in photosynthetic organisms via photocatalysis by the Oxygen Evolving Complex (OEC) of Photosystem II. Mononuclear water oxidation catalysts that aim to mimic nature typically rely on heavy, rare metals such as ruthenium and iridium. Replacing these metals with iron is particularly appealing because it is abundant, benign, and inexpensive. We use density functional theory to characterize the catalytic ability of …

Do Microplastics, Vibrio Bacteria, And Warming Water Temperatures Cause Disease In The Northern Star Coral, Astrangia Poculata?, Heather Leigh Sheffey

OES Theses and Dissertations

As marine plastic pollution increases, it becomes imperative to study the effects of microplastics on marine fauna. Marine plastic debris is a vector for disease to marine invertebrates (Lamb et al., 2018; Rotjan et al., 2019). In this context, studies have shown Astrangia poculata will consume microplastics as they do their normal food (Allen et al, 2017; Rotjan et al., 2019). Further, with temperatures increasing worldwide, there is a concern the virulence of disease-causing bacteria will increase (Vezzulli et al., 2015). In a series of manipulative laboratory experiments, this study quantified changes in respiration rates and visual health of Astrangia …

Organic Carbon Decay Mediated By Mesopelagic Microbial Communities, Noah Jonathan Craft

OES Theses and Dissertations

Substantial remineralization of organic carbon occurs in the mesopelagic zone (i.e., the biological pump), the efficiency of which is responsible for the oceans’ capacity to store carbon originally derived from the atmosphere. To better understand how a substrate’s composition influences its degradation by mesopelagic microbial communities, we added treatments made of live algal cells, dead particulate organic carbon and dissolved organic carbon from ¹⁴C-labeled algal cultures to mesopelagic water collected in-situ. Each incubation took place in the laboratory over a period of months, during which PO¹⁴C, DO¹⁴C, ATP, prokaryote abundances and biochemical fractions were measured. …

A Reflection Of Experiences Of Adults With Type 1 Diabetes In Integrated Physical Education Classes, Kalleigh West

Human Movement Studies & Special Education Theses & Dissertations

The purpose of this study was to gain an understanding of the experiences of type 1 diabetics in integrated physical education classes. In this study, we interviewed young type 1 diabetic adults and asked them to reflect on their school-based physical education experiences. An interpretative phenomenological analysis (IPA) approach was adopted to guide data collection, analysis, and interpretation for this retrospective study. Eight participants (ages 19 to 32) were enrolled in this study, and semi-structured interviews focused on their physical education experiences acted as the primary data. Transcribed interview data were analyzed using an IPA approach. Three interrelated themes emerged …

Tracing And Segmentation Of Molecular Patterns In 3-Dimensional Cryo-Et/Em Density Maps Through Algorithmic Image Processing And Deep Learning-Based Techniques, Salim Sazzed

Computer Science Theses & Dissertations

Understanding the structures of biological macromolecules is highly important as they are closely associated with cellular functionalities. Comprehending the precise organization of actin filaments is crucial because they form the dynamic cytoskeleton, which offers structural support to cells and connects the cell’s interior with its surroundings. However, determining the precise organization of actin filaments is challenging due to the poor quality of cryo-electron tomography (cryo-ET) images, which suffer from low signal-to-noise (SNR) ratios and the presence of missing wedge, as well as diverse shape characteristics of actin filaments. To address these formidable challenges, the primary component of this dissertation focuses …

Effect Of Acute Caffeine Ingestion On On-Field Performance In Division 1 Female Field Hockey Players, Nicole Danielle Fredricks

Human Movement Studies & Special Education Theses & Dissertations

This study aimed to determine the effects of acute caffeine ingestion on on-field performance in collegiate female field hockey players. A placebo-controlled, double-blind, randomized, crossover experimental design was utilized. The participants (N=10) were female division 1 field hockey players (age: 20.0 ± 1.6 years). They competed in two 60-minute, off-season scrimmages separated by two weeks. They were randomly assigned to ingest the caffeine, 3 mg/kg of body weight, or placebo treatment 60 minutes before each scrimmage. They wore a PLAYERTEK (Catapult, Australia) global positioning system (GPS) device to track their total distance, sprint distances, power plays, and zone three and …

Characterizing Allosteric Regulation And Conformational Dynamics In Staphylococcus Aureus Pyruvate Carboxylase, Amanda Joan Laseke

Dissertations (1934 -)

Pyruvate carboxylase (PC) produces oxaloacetate from pyruvate and bicarbonatein an ATP-dependent manner. The catalytic activity of PC places it at a pivotal intersection between catabolism and anabolism. Understanding the essential metabolic role of PC requires a more complete description of how its activity is regulated and how that regulation is manifested through changes in conformational dynamics. PC activity is regulated by the mutually exclusive binding of the allosteric activator, acetyl-CoA, and the allosteric inhibitor, L-aspartate. The binding site for acetyl- CoA has largely been identified, but the binding location for the acetyl moiety is unknown. Given that the acetyl moiety …

Probiotic Compounds Inhibit Staphylococcus Aureus Biofilm Formation, Reduce Virulence, & Improve Antibiotic Sensitivity, Kyle R. Leistikow

Dissertations (1934 -)

Multidrug-resistant (MDR) bacterial infections are a top five global public health threat, causing 2.8 million infections and 35,000 deaths annually in the US alone. Staphylococcus aureus is one of the most clinically important MDR pathogens in the world with infections leading to high rates of morbidity and mortality in both humans and animals. This bacterium's ability to form protective biofilms further complicates classical antibiotic interventions, highlighting the need for new therapeutics with novel mechanisms of action. The goal of this dissertation was to investigate antibiofilm mechanisms employed by probiotic bacteria to reduce S. aureus virulence and mitigate antimicrobial resistance evolution …

Modified Vaccine Vectors To Understand Adjuvant Functions Of Listeria During Chronic Schistosomiasis, Stephanie K. Norwood

Biological Sciences Theses & Dissertations

Vaccination is one of the most effective strategies employed to prevent infectious diseases. Successful vaccination is dependent upon the induction of a specific, robust, and prolonged immune response. One of the major challenges faced by vaccine development is vaccine failure due to host-related factors that can modulate the immune system, which leads to non-responsiveness to vaccinations. The generation of new vaccine strategies is imperative to combat these effects. Live bacterial vectors are one approach used as they can elicit humoral immunity, cellular immunity, or both. Listeria monocytogenes is a Gram positive, intracellular pathogen that is an effective bacterial vaccine vector …

Dental Hygiene Students Reported Physiological Symptoms Associated With Wearing An N95 Respirator Mask, Peyton Shea Butler

Dental Hygiene Theses & Dissertations

Purpose: Physiological symptoms and comfort levels while wearing an N95 respiratory mask has not been examined with dental hygienists. The purpose of this study was to investigate dental hygiene students reported physiological symptoms and comfort perception while wearing an N95 respirator mask during patient care appointments. Methods: After IRB approval (IRB #1987754-2), a 16-item questionnaire was distributed through email to a convenience sample of 65 dental hygiene students. Questions assessed respiratory, dermatologic, cardiac, mask mouth and general physiological symptoms, as well as comfort levels. Additionally, participants were asked to respond to demographic questions and one open ended question inquiring about …

Age, Lower Extremity Muscle Strength, And Running Biomechanics In Healthy Female Recreational Runners, Heather Mcconchie Hamilton

Rehabilitation Sciences Theses & Dissertations

Middle-age and older runners demonstrate differences in running biomechanics compared to younger runners. Females and males demonstrate differences in running biomechanics, and females experience additional hormonal changes with age due to menopause. Despite the biomechanical and physiological differences between females and males, little research has investigated the effect of age specifically in female runners. The overall purpose of this research was to determine the relationships among age, lower extremity muscle strength, and running biomechanics in healthy female recreational runners. Healthy female recreational runners aged 25 – 65 years were recruited. Participants ran on an instrumented treadmill at a training pace …

The Effects Of Lifting With Intent On Hip And Knee Moments During Back Squats, Kyle A. Parrish

Human Movement Studies & Special Education Theses & Dissertations

Back squatting is a widely used movement in the strength and conditioning community. Within the last few years, velocity-dependent training and its application of tracking intensity have increased in popularity. Biomechanics research has frequently examined force generation, joint loading, and muscle contributions of back squats with different bar placement, stance width, and increasing barbell loads. However, no information exists regarding whether lifting with intent increases velocity and how increased velocity is biomechanically achieved. This study aimed to examine how lifting with intent affects bar velocity and how joint moment alterations could contribute to these potential changes in velocity. Fifteen males …

Structural Insights Into The Cl-Par-4 Protein: Ionic Requirements, Conformational Transitions, And Interaction With Cisplatin, Krishna Kumar Raut

Chemistry & Biochemistry Theses & Dissertations

Cancer continues to be the leading global cause of death, with challenges in early diagnosis, drug resistance, non-specific drug targeting, and cancer recurrence and metastasis posing formidable obstacles in cancer therapy. In this context, Prostate Apoptosis Response-4 (Par-4), a pro-apoptotic tumor suppressor protein, emerged as a promising therapeutic target due to its ability to selectively induce apoptosis in cancer cells, thereby minimizing the drug-associated adverse effects. However, a comprehensive understanding of the structural features of Par-4, specifically the caspase-cleaved fragment (cl-Par-4), is crucial for therapeutic advancements.

This dissertation investigated the effects of various ions, both monovalent and divalent, on the …

A Blunted Th17 Cytokine Signature In Women With Mild Cognitive Impairment: Insights From Inflammatory Profiling Of A Community-Based Cohort Of Older Adults, Adam D. Bachstetter, Jenny Lutshumba, Edric D. Winford, Erin L. Abner, Barbra J. Martin, Jordan P. Harp, Linda J. Van Eldik, Frederick A. Schmitt, Donna M. Wilcock, Ann M. Stowe, Gregory A. Jicha, Barbara S. Nikolajczyk

Markey Cancer Center Faculty Publications

People with dementia have an increase in brain inflammation, caused in part by innate and adaptive immune cells. However, it remains unknown whether dementia-associated diseases alter neuro-immune reflex arcs to impact the systemic immune system. We examined peripheral immune cells from a community-based cohort of older adults to test if systemic inflammatory cytokine signatures associated with early stages of cognitive impairment. Human peripheral blood mononuclear cells were cultured with monocyte or T-cell-targeted stimuli, and multiplex assays quantitated cytokines in the conditioned media. Following T-cell-targeted stimulation, cells from women with cognitive impairment produced lower amounts of TH17 cytokines compared with cells …

Control Of Cd4+ T Cells To Restrain Inflammatory Diseases Via Eukaryotic Elongation Factor 2 Kinase, Hao-Yun Peng, Liqing Wang, Jugal Kishore Das, Anil Kumar, Darby J. Ballard, Yijie Ren, Xiaofeng Xiong, Paul De Figueiredo, Jin-Ming Yang, Jianxun Song

Markey Cancer Center Faculty Publications

CD4+ T cells, particularly IL-17-secreting helper CD4+ T cells, play a central role in the inflammatory processes underlying autoimmune disorders. Eukaryotic Elongation Factor 2 Kinase (eEF2K) is pivotal in CD8+ T cells and has important implications in vascular dysfunction and inflammation-related diseases such as hypertension. However, its specific immunological role in CD4+ T cell activities and related inflammatory diseases remains elusive. Our investigation has uncovered that the deficiency of eEF2K disrupts the survival and proliferation of CD4+ T cells, impairs their ability to secrete cytokines. Notably, this dysregulation leads to heightened production of pro-inflammatory cytokine IL-17, fosters a pro-inflammatory microenvironment …

Copy Number Variation That Influences The Ionizing Radiation Sensitivity Of Oral Squamous Cell Carcinoma, Tadahide Izumi, Piotr G. Rychahou, Li Chen, Molly H. Smith, Joseph Valentino

Markey Cancer Center Faculty Publications

Genome instability in cancer cells causes not only point mutations but also structural variations of the genome, including copy number variations (CNVs). It has recently been proposed that CNVs arise in cancer to adapt to a given microenvironment to survive. However, how CNV influences cellular resistance against ionizing radiation remains unknown. PRMT5 (protein arginine methyltransferase 5) and APE1 (apurinic/apyrimidinic endonuclease 1), which enhance repair of DNA double-strand breaks and oxidative DNA damage, are closely localized in the chromosome 14 of the human genome. In this study, the genomics data for the PRMT5 and APE1 genes, including their expression, CNVs, and …

Dense Granule Protein 3 Of Toxoplasma Gondii Plays A Crucial Role In The Capability Of The Tissue Cysts Of The Parasite To Persist In The Presence Of Anti-Cyst Cd8+ T Cells During The Chronic Stage Of Infection, Rajesh Mani, Mohamed H. Abdelaziz, Eri Ochiai, Qila Sa, Barbara A. Fox, David J. Bzik, Yasuhiro Suzuki

Markey Cancer Center Faculty Publications

Toxoplasma gondii establishes chronic infection by forming tissue cysts, and this chronic infection is one of the most common parasitic infections in humans. Our recent studies revealed that whereas CD8 + T cells of genetically resistant BALB/c mice have the capability to remove the tissue cysts of the parasite through their perforin-mediated activities, small portions of the cysts are capable of persisting in the presence of the anti-cyst CD8+ T cells. It is currently unknown how those small portions of the cysts resist or escape the T-cell immunity and persist in the hosts. In the present study, we discovered that …

Distinct Camp Signaling Microdomains Differentially Regulate Melanosomal Ph And Pigmentation, Maftuna Yusupova, Dalee Zhou, Jaewon You, Jeydi Gonzalez-Guzman, Megha B. Ghanta, Hong Pu, Zalfa Abdel-Malek, Qiuying Chen, Steven S. Gross, John August D'Orazio, Shosuke Ito, Kazumasa Wakamatsu, Melissa L. Harris, Jonathan H. Zippin

Markey Cancer Center Faculty Publications

cAMP signaling is a well-established regulator of melanin synthesis. Two distinct cAMP signaling pathways—the transmembrane adenylyl cyclase pathway, activated primarily by the MC1R, and the soluble adenylyl cyclase (sAC) pathway—affect melanin synthesis. The sAC pathway affects melanin synthesis by regulating melanosomal pH, and the MC1R pathway affects melanin synthesis by regulating gene expression and post-translational modifications. However, whether MC1R genotype affects melanosomal pH is poorly understood. We now report that loss of function MC1R does not affect melanosomal pH. Thus, sAC signaling appears to be the only cAMP signaling pathway that regulates melanosomal pH. We also addressed whether MC1R genotype …