Life Sciences Commons^™

Chemical Biology Approach To Study Rna Modifications, Ya Ying Zheng

Legacy Theses & Dissertations (2009 - 2024)

Nucleic acid modifications have gained compelling interest for their pivotal roles in cellular and biological processes. These modifications introduced a new layer of regulation without altering the original genetic sequence. In this burgeoning field of epitranscriptomics, many of the chemical modifications have emerged as potential biomarkers in therapeutic applications. Inosine is an essential post-transcriptional RNA modification that has functional roles in modulating the fate of all RNAs. The enzyme ADAR catalyzes the deamination of adenosine to inosine. This can also lead to change in the eukaryotic transcriptome due to A-G miscoding since inosine preferentially base pairs with cytosine. Moreover, hyper-editing …

Loss Of Courtship Suppression Memory In A Drosophila Melanogaster Model Of Alzheimer’S Disease, Eric Robles, Johannes Berlandi, Chris Ellis, Tianyi Wu, Astrid Jeibmann, Fang-Ju Lin

Biology

Alzheimer’s disease (AD) is the most prevalent and lethal neurodegenerative disease. Memory loss and motor dysfunction are accompanied by pathological hallmarks like neurofibrillary tangles or amyloid plaques. In this study, courtship suppression assay was used to assess learning and memory of a transgenic Drosophila melanogaster (the fruit fly) line expressing human Amyloid beta 42 (Aβ42). At young age (4–6 days old), both parental control and AD flies displayed lower courtship indices during training after being rejected by previously mated females. However, in the subsequent testing phase, young AD flies showed compromised recall memory, unlike that of parental controls. Neither control …

Elucidation Of Mismatch Repair Regulation By Abl1: Advantages/Disadvantages Of Tyrosine Kinase Inhibitor Treatment, Hannah Daniels

Theses and Dissertations--Toxicology and Cancer Biology

DNA mismatch repair (MMR) is a critical repair process necessary for not only repairing mispairs incorporated into the DNA during replication, but also for inducing apoptosis in response to certain types of DNA damage. MMR is required for maintaining genomic integrity and due to the importance of this pathway, any impairment of its function can lead to increased mutation frequency, which is well known to be a driving force of cancer development, progression, and resistance. Despite extensive studies involving the proteins involved in the MMR pathway, the regulation of those proteins remains relatively unknown. Regulation of MLH1, a protein necessary …

Acquired Treatment Resistance In Prostate Cancer Via The Production Of Radiation Derived Extracellular Vesicles Containing Mitochondrial Proteins, Caitlin Miller

Theses and Dissertations--Toxicology and Cancer Biology

Radiation treatment (RT) is one of the most commonly administered treatments for prostate cancer (PCa) patients, yet the recurrence of tumors after RT remains one of the significant challenges in the eradication of PCa. In addition to induction of DNA damage, ROS production, and cell death, we found that RT promotes Extracellular vesicle (EV) production in PCa cells, at least in part, through H₂O₂ production (Miller CE et al., Antioxidants 2022, PMID 36358489). Since we observed EV production upon RT, EVs and their contents could play a role(s) in cancer survival after RT. By using flow cytometry …

Porcupine’S Role In The Enhancement Of Enzalutamide Efficacy In Drug Resistant Prostate Cancer, Katelyn Jones

Theses and Dissertations--Toxicology and Cancer Biology

Androgen receptor (AR) signaling continues to participate as a vital component of castration-resistant prostate cancer (CRPC). Subsequently, this has led to the development of Androgen Signaling Inhibitors (ASI), specifically Enzalutamide (ENZ), which is a direct inhibitor of AR, to clinically manage CRPC. Inevitably, ENZ treatment only provides improvement for approximately two months before advancing to an incurable form, ENZ-resistant CRPC. With PCa ranking as the second leading cause of cancer-related deaths in USA males, there is an urgency and necessity for the discovery and development of novel therapeutic approaches for CRPC. Wnt signaling has been extensively documented in its involvement …

Therapeutic Approaches And Novel Mechansims In Cancer Progression, Kendall Simpson

Theses and Dissertations--Toxicology and Cancer Biology

Prostate cancer (PCa) remains the most diagnosed cancer among men in the United States. There are various therapeutic routes that are implored to combat this fatal disease, and our work aims to increase the options made available to PCa patients. A combination treatment of Enzalutamide (Enz), an FDA approved drug for castration-resistant PCa patients, and Metformin, an FDA approved drug for type 2 diabetes, was utilized to enhance the efficacy of Enz in Enz-resistant PCa, both in vitro and in vivo. Thymic Lymphoma is one of the most common malignancies that can occur in various tissues and organs. Genomic stability …

Delineating Contributions Of Genotype And Lineage To Lung Cancer Therapy Response, Kassandra Jo Naughton

Theses and Dissertations--Toxicology and Cancer Biology

Non-small cell lung cancer (NSCLC) heterogeneity is a major challenge for determining effective treatment strategies. Adenocarcinomas (ADCs) and squamous cell carcinomas (SCCs) are histologically and epigenetically distinct subtypes of NSCLC. Patients with ADC tumors harboring mutations in both KRAS and LKB1 (aka STK11) have lower survival rates than those with KRAS-only tumors. KRAS/LKB1 tumors are not only aggressive, but also respond poorly to immunotherapy. However, these data are limited to ADCs, and it is unclear if SCCs with this genotype are also resistant to immunotherapy. We developed a mouse model of Kras^mut/Lkb1^mut capable of producing …

Rps6kb1 Is A Critical Target For Overcoming Tumor Lineage Plasticity And Therapy Resistance, Saptadwipa Ganguly

Theses and Dissertations--Toxicology and Cancer Biology

Most tumors initially respond to treatment, yet refractory clones subsequently develop owing to resistance mechanisms which can be classified as intrinsic or extrinsic resistance. The intrinsic resistance is usually present prior to any treatment whereas the extrinsic resistance develops as a result of exposure to some kind of therapy. In order to target these “therapy resistant” tumor cells an unbiased screen of an FDA-approved drug library against cancer cells was performed. This was followed by synthesis of chemical analogs of the most effective drug.

The studies led to the identification of a derivative of the antihistamine drug ebastine, designated Super-ebastine …

Enabling Improved Hand Function In Disabled Individuals Via Intention Estimation And Suppression Of Disrupted Central Drive, Hendrik Adriaan Dewald

Doctoral Dissertations

No abstract provided.

Polymorphism And Plasticity In The Termite Caste System, Austin Merchant

Theses and Dissertations--Entomology

Developmental plasticity, manifested as a caste system, is integral to the success of eusocial insects because of the way in which it decouples the various tasks that a single, solitary organism would normally be responsible for, allowing individuals to specialize towards their given roles while keeping vulnerable members of the colony, such as those responsible for reproduction, safe from harm. Termites exhibit particularly complex caste systems that can be divided into three types of castes: workers perform manual labor, such as foraging and brood care; soldiers defend the nest; and reproductives lay eggs. Each of these castes, as well as …

An Rnai-Based Approach To Ips Calligraphus Management: A Promising Strategy For Forest Protection, Mary Wallace

Theses and Dissertations--Entomology

As climate change leads to more frequent and severe extreme weather, host tree stress is increasing the occurrence of large-scale bark beetle outbreaks. One such beetle, Ips calligraphus (Germar), a native bark beetle in the southeastern United States, has traditionally been considered a secondary pest of pine trees. Management has relied on silvicultural practices that enhance forest resilience, enabling host trees to effectively use natural defenses to deter beetle infestation. However, during periods of forest stress caused by storms, droughts, human disturbance, and other factors, beetle outbreaks can cause considerable ecological and economic damage.

Addressing worsening I. calligraphus outbreaks will …

Theoretical And Experimental Constraints For The Equation Of State Of Dense And Hot Matter, Rajesh Kumar, Veronica Dexheimer, Johannes Jahan, Jorge Noronha, Jacquelyn Noronha-Hostler, Claudia Ratti, Nico Yunes, Angel Rodrigo Nava Acuna, Mark Alford, Mahmudul Hasan Anik, Debarati Chatterjee, Katerina Chatziioannou, Hsin Yu Chen, Alexander Clevinger, Carlos Conde, Nikolas Cruz-Camacho, Travis Dore, Christian Drischler, Hannah Elfner, Reed Essick

All Faculty Open Access Publications

This review aims at providing an extensive discussion of modern constraints relevant for dense and hot strongly interacting matter. It includes theoretical first-principle results from lattice and perturbative QCD, as well as chiral effective field theory results. From the experimental side, it includes heavy-ion collision and low-energy nuclear physics results, as well as observations from neutron stars and their mergers. The validity of different constraints, concerning specific conditions and ranges of applicability, is also provided.

Developing A Methodology For Evaluating Postural Control And Functional Ability In Children With Cerebral Palsy, Abigail Schoppa

Honors College Theses

Background: Cerebral Palsy (CP) is a non-progressive group of disorders that interferes with postural control and gross motor function (GMF). The purpose of this study was to develop a methodology to evaluate and link postural control and functional ability by identifying the best measures to use.

Methods: For this study, children between the ages of 12 and 22 who have CP and a Gross Motor Function Classification System level I to III were recruited. They performed a series of gross motor tests from the BOT-2 while standing on a force plate to record their center of pressure (COP) movement. Several …

A Tale Of Two Turtles: Sympatry Of Two Lotic Musk Turtle Species In The Pascagoula River Drainage, Grover J. Brown, Brian R. Kreiser

Research, Publications & Creative Work

Competition and hybridization between closely related species have remained topics of interest for decades. The ranges of the Razorback Musk Turtle (Sternotherus carinatus) and Stripe-necked Musk Turtle (Sternotherus peltifer) are mostly non-overlapping, but they are sympatric in south and central Mississippi, USA. In allopatry, each species fills the niche of a lotic, bottom-walking musk turtle and each can range in habitat from small, montane headwater streams to large, muddy bayous in the coastal plain. Using environmental, morphological, and genetic data, we assessed whether the two species exhibit different habitat associations, differ in morphology, and maintain reproductive isolation in sympatry. The …

Combination Of Hdac And Fyn Inhibitors In Synovial Sarcoma Treatment, Kyra Parker, Yanfeng Zhang, Gavin Anchondo, Ashlyn Smith, Sergio Guerrero Pacheco, Tadashi Kondo, Le Su

Research, Publications & Creative Work

The SS18-SSX fusion protein is an oncogenic driver in synovial sarcoma. At the molecular level, SS18-SSX functions as both an activator and a repressor to coordinate transcription of different genes responsible for tumorigenesis. Here, we identify the proto-oncogene FYN as a new SS18-SSX target gene and examine its relation to synovial sarcoma therapy. FYN is a tyrosine kinase that promotes cancer growth, metastasis and therapeutic resistance, but SS18-SSX appears to negatively regulate FYN expression in synovial sarcoma cells. Using both genetic and histone deacetylase inhibitor (HDACi)-based pharmacologic approaches, we show that suppression of SS18-SSX leads to FYN reactivation. In support …

Acknowledgment Note

Mansoura Engineering Journal

No Abstract

Production Of Hydrogen From Sodium Borohydride Using Bimetal Catalyst, Haitham I. Attia, Hani Mahanna, M.M. El-Halwany, Mahmoud H Mahmoud

Mansoura Engineering Journal

Here, in this paper we try to focus on the bi-metal non-semiconductor photo catalyst for the Production of hydrogen from sodium borohydride instead of semi conductor photo catalyst which have an excellent performance in photo-catalysis. In this paper , we studied the combination of CuNi bi-metal(developed and reinforced on MWCNTs) as a non-semiconductor photo-catalyst that generates electrons for the Production of hydrogen from sodium borohydride with led light as source of light , We also added CuNi on multi wall carbon nano tubes (MWCNTs) for increasing the surface area of the reaction. We studied the effect of different quantity of …

Predicting The Role Of Connectivity In The Development Of Border Regions In Egypt, Shimaa Mohammed Hamdy Derbala

Mansoura Engineering Journal

The research analyzed the current situation of the development of 12 existing roads in the development plan 2052 and linked to the Governors of (Matrouh, North Sinai, South Sinai, New Valley, and Red Sea), and by reviewing previous studies, it showed the link between road development and the demographic and social factors of the population. The methodology for studying various cases and the methodology for predicting development models are the most appropriate for the field of research. This research examines the goals and challenges of road development with global experience that can be applied to development plans. The research is …

Application Of Machine Learning Models In The Capacity Prediction Of Ccfst Columns, Khaled Megahed, Nabil Said Mahmoud, Saad Elden Mostafa Abd-Rabou

Mansoura Engineering Journal

Circular concrete-filled steel tubular (CCFST) columns are widely utilized in structural engineering due to their impressive load-bearing capabilities and ductility. Existing design standards often yield disparate outcomes when applied to structural columns with identical properties, introducing uncertainty for engineering designers. This study introduces an innovative technique to address these challenges using two machine learning (ML) models: Gaussian process regression (GPR) and extreme gradient boosting (XGBoost). These models consider various input variables, including the geometric and material properties of CCFST columns, to estimate the compressive strength. The models undergo training and evaluation using two datasets comprising 1004 axially loaded CCFST columns …

An Enhanced Lane Detection Technique Based On Active Learning, Ahmed M. Radwan, Amira Y. Haikal, Hisham E. Gad, Mohamed M. Abdelsalam

Mansoura Engineering Journal

The lane detecting algorithm plays a major role in advanced driver assistance systems (ADAS) and autonomous driving systems. In recent years, deep learning-based lane detection techniques have shown encouraging results; nonetheless, the quality and size of the training data set has a significant impact on how effective these techniques are. Active learning is a technique that can improve the capacity of deep learning-based lane identification systems to repeatedly choose and classify valuable samples from a large body of unlabeled data. In this research, a novel 1-dimensional deep learning approach is used to present an augmented Active Learning based Lane Detection …

Effect Of The Shape And Volume Of Capsulated Phase Change Materialon The Melting Process And Exergy Efficiency, Ahmed Saad Soliman, Ahmed A. Sultan, Mohamed A. Sultan

Mansoura Engineering Journal

Due to the lack of solar energy continuity throughout the day, researchers tended to store this energy in phase change materials (PCM). The goal is to store a big amount of energy in a small PCM mass. In this study, the effect of the capsule shape (circular and elliptical cross-section), number of capsules with the same volume (1, 2, 3, 4, and 5 capsules), and one capsule with increasing its initial volume (multiplying of volume ratio 1 (Vr = 2, 3, 4, and 5)) on the PCM melting process was studied. The results of the numerical model for both circular …

A Novel Method For The Production Of Pig Iron In “Hadisolb” Company (Industrial-Scale Experiments), El-Sayed A. Rassoul

Mansoura Engineering Journal

The chairman of “Hadisolb” had submitted a memorandum in 1977 in which he claimed that the company will never achieve its designed capacity unless it gets rid of the threatening impurities -high alkalis & MnO- in the blast furnace burden. Meanwhile, the DR steel companies are suffering from the generated accumulated wastes which contain > 67% Fe, zero% alkalis and traces of MnO. The novel idea here is finding the best ratio between El-Gedida ore and the waste material (W.M.). In a previous investigation a series of pilot-scale sintering experiments were performed and it was concluded that the blend containing 70% …

The Mechanical Performance Of Sustainable High Performance Concrete Containing Hybrid Polypropylene Fibers And Exposed To Elevated Temperatures, Ahmed M. Tahwia, Marwa M. Ouda, Mohamed Abdellatief, Walid E. Elemam

Mansoura Engineering Journal

The purpose of this research is to investigate the impact of hybrid micro and macro polypropylene fibers (PPFs) on the mechanical characteristics and microstructure of high performance concrete (HPC). Seven concrete mixes with different contents of hybrid polypropylene fibers were tested. The tests included the slump test, compressive and flexural strength, resistance to elevated temperatures, and concrete microstructure. Compared to the control mixture, introducing macro and micro polypropylene hybrid fibers to HPC mixes dramatically enhanced the flexural and compressive strength of concrete while lowering workability. HPC compressive and flexural strengths decrease as temperature rises, nevertheless, the strength loss rate was …

Retrofitting The Building Materials And Techniques Of A Residential Villa: Finding Alternative Solutions, Ahmed Abdelmonteleb M. Ali, Essam S. Almahmoud, Azzam I. Aljutayli, Basem O. Elgendy, Meshal A. Alhrabi, Khattab I. Alyahya

Mansoura Engineering Journal

Approximately 40% of greenhouse gas emissions globally are attributed to the construction industry, with building materials alone accounting for 11%. This study evaluated several building materials and techniques to investigate alternative possibilities for retrofitting a residential villa. The research methodology thoroughly evaluated the building materials and techniques employed in a Saudi villa as a case study located in Buraidah within the Al-Qassim Region. This examination included the structural integrity and cost comparison between the current and suggested scenarios. Alternative building materials and methods are discovered, and their possible application in retrofitting the villa is examined based on this evaluation. The …

Unleashing The Potential: The Impact Of Biophilic Office Design On Enhancing Employee Productivity, Asmaa Elantary

Mansoura Engineering Journal

The observed landscape influences people in numerous ways, including aesthetic appreciation, health, and well-being. The evidence of health benefits associated with viewing those landscapes has proven exceptional value. A comprehensive review of papers on landscapes and sound effects on health was conducted. According to a growing body of evidence, exposure to visible landscapes positively affects one's health and fitness. The most pressing social and economic challenge now is increasing worker productivity, attracting, and retaining exceptionally talented employees, and improving overall employee performance. This will not happen unless companies provide a safe, comfortable, and stimulating work environment; this will not happen. …

Glaucoma Diagnosis Using Hybrid Neural Encoder Decoder Based Unet Hybrid Inception, Akanksha Bali, Vibhakar Mansotra

Mansoura Engineering Journal

The anatomy of the retinal fundus picture, which includes the optic disc, affects how glaucoma characteristics are extracted. One of the main causes of blindness in the globe is glaucoma. Correct glaucoma screening in its early phases is difficult since the condition only becomes apparent when its symptoms are severe. If glaucoma is not treated, the optic nerve head (ONH) will be harmed, resulting in vision loss. Nevertheless, there are currently not enough eye experts accessible, and eye screening is personal, time-consuming and labor-intensive. Therefore, this is a real issue that can be addressed by automatically diagnosing glaucoma using deep …

The Transformer Modelling For Vftos Calculation - A Comprehensive Review, Mohamed Salah, Ebrahim A. Badran, Mansour H. Abdelrahman

Mansoura Engineering Journal

The transformer is exposed to Very fast transient overvoltages (VFTOs) originated from the switching operations in the gas insulated switchgears (GIS), these overvoltages may damage the transformer insulation and result in catastrophic failures. Consequently, the transformer should be modeled to handle the transient state. In this work, the methods published in the literature to illustrate the VFTOs in GIS are reviewed, and then an overview of the transformer modelling techniques is presented with the contribution and shortcomings of each technique. The determination methods of the parameters required for the modelling of the transformer are also reviewed. In addition, the contributions …

Vertical Farming: A Key To Sustainable Compact Cities, Mohab Taher Abdelfatah, Sahar Mahmoud El-Arnaouty, Akram Abdelhakim Zayan

Mansoura Engineering Journal

Urbanization continues to surge, with projections indicating that by 2050, around 70 percent of the global population will reside in urban areas. In response, urban planners are exploring novel urban paradigms to ensure food security through urban agriculture, a cornerstone of sustainable development encompassing environmental, economic, and social dimensions. This study addresses a critical research gap within urban agriculture, conducting a comparative analysis between horizontal and vertical farming methodologies within urban contexts. By categorizing various typologies of vertical farming, from container farms to mixed-use structures, this research highlights their distinct contributions to sustainable urban development. Emphasis is placed on vertical …

Assessing The Utilization Of Glazing Types And Alternatives In Office Buildings To Reduce Energy Consumption And Carbon Emissions, Eman Badawy Ahmed

Mansoura Engineering Journal

According to Egypt's Vision 2030 and the recommendations of the Climate Conference, Cop 27, Egypt seeks to reduce energy consumption and emissions, especially carbon, by calculating the carbon footprint and moving towards issuing carbon certificates.
The elements of the building envelope have a strong impact on energy consumption and carbon emissions. Glass is one of the most influential elements. Therefore, it is necessary to study the effect of glassing on energy consumption.
The study aims to evaluate the use of glazing types and their alternatives to reduce energy and carbon consumption in administrative buildings by developing an estimation approach for …

Knowledge Gap Analysis For Efficient Form-Finding Of Lightweight Structures, Shorouk Mohamad Khedr, Mohamed Esmat Elattar, Ahmed Eltantawy, Esraa Elazab

Mansoura Engineering Journal

Historically, Performance assessment of temporary structures’ form was substantially dependent on how loads transfer along geometry. Fortunately, the development of tools has enabled designers to explore more design variations as well as testing their feasibility. This study aims to address the knowledge gap between design process, tool contributions and feasibility requirements of temporary structures. This will be achieved through analyzing the evolution of temporary structures’ design approaches including early form finding approaches, geometrical properties dependencies, reflections of both bottom -top and top-bottom approach as well as linking them to material innovations, structural contributions and manual experiments. As a result, the …