Life Sciences Commons^™

Ants And Their Response To Chili Powder, Antu Biswas

2024 REYES Proceedings

Spices can be used as an eco-friendly ant repellent. In this study, I tried to observe the ants’ response to chili powder and whether or not the spice can be an effective ant repellent.

The Efficacy Of Dry Peppermint Leaves As An Ant Repellent, Amal Chacko

2024 REYES Proceedings

Ant (Formicidae) infestation is a typical residential disturbance, often necessitating the use of chemical repellents that pose environmental and health dangers. This study looks into the possibilities of a natural alternative, dry peppermint (Mentha piperita L) leaves as a chemical repellent. Given the diversity of ant species and their variable responses to stimuli, this study seeks to determine the efficiency of dried peppermint leaves as a general ant repellent. A pilot study will be carried out to determine the repellent qualities of this spice against a specific ant species. The precise aims include evaluating if dry peppermint leaves …

Pharmacokinetic Modeling Of Metonitazene And Isotonitazene Using In Vitro And In Vivo Models And Comparison To Human Postmortem Casework, Justine P. Sorrentino

Theses and Dissertations

Metonitazene and isotonitazene are two benzimidazole opioids that are contributing to the opioid crisis nationwide. Limited pharmacokinetic data has been published on these nitazene compounds, therefore this study sought to determine if these two structurally similar opioids would have comparable half-lives and clearance utilizing in vitro and in vivo models. Using the data determined in the pharmacokinetic study, and by testing human postmortem cases, this study aimed to inform interpretation of these drugs detected in human casework. Sample analysis for all aspects of the study was performed utilizing LC/MS/MS. In vitro pharmacokinetics of both drugs were examined using a human …

Streptozotocin-Induced Diabetes Increases Kidney Toxicity In Rats Exposed To Ochratoxin A, Belkys Gonzalez

Theses and Dissertations

Due to the rising occurrence of diabetes in the population and the prevalence of OTA exposure via ingestion of contaminated foodstuffs, there is a need to assess the possible renal interaction of a diabetic disease state in concurrence with OTA exposure. Sprague Dawley rats were treated with streptozotocin to induce experimental diabetes and then orally exposed to 0.5 mg/kg body weight OTA once a day for 21 days. Renal biomarkers and light microscopy were evaluated to assess the interaction between diabetic nephropathy and OTA exposure. LLC-PK1 proximal tubule cells were used as an in vitro model to assess the potential …

Exposure To Dithiocarbamate Fungicide Ziram Results In Hepatic And Renal Toxicity In Long-Evan Rats, Jeffrey Chen

Theses and Dissertations

Ziram is a broad spectrum fungicide that is used on a variety of crops. It has been shown to display a wide array of toxicological effects, however, there is still a scarcity of mechanistic data regarding how it facilitates those effects. One of its presumed mechanisms of toxicity is due to the production of oxidative stress. However, whether the production of oxidative stress is due to its dimethyldithiocarbamate backbone, its metal moiety, or a combination of both, is still unknown. Contributing to the production of oxidative stress is ziram’s ability to alter essential metal homeostasis through transchelation of other essential …

Determination Of Genetic Variation In Opioid-Resistant Hospice Patients Using A Pharmacogenomics Approach, Daniel Bianculli

Theses and Dissertations

Managing pain requires a delicate balance between the advantages of opioid analgesia and the possible adverse effects. This balance becomes even more difficult in hospice and palliative care environments where dosage regimens are frequently ambiguous. Some hospice patients experience opioid resistance, in which opioids no longer produce an analgesic effect on a patient. Evidence-based analyses frequently omit this vulnerable population, resulting in a need for concrete procedures. The emergence of pharmacogenomic analysis and personalized treatment presents a solution to this problem by using each patient's genomic data to interpret opiate dosing. This pharmacogenomic study aimed to single nucleotide polymorphisms (SNPs) …

Production Of Vp3-Only Aav2 Vlp In E. Coli, Chengyu Fu

Theses and Dissertations

Adeno-associated virus (AAV) is a widely used vector for gene therapy. However, few studies have focused on producing AAV-based virus-like particles (VLP) in E. coli. This project aimed to express VP3 of AAV2 or co-express VP3 of AAV2 with other proteins, such as E. coli chaperones and/or assembly-activating protein (AAP) in E. coli under different expression conditions and explore the potential to produce VP3-only VLP of AAV2 in situ in E. coli. The constructed plasmid pET15ΔHis-VP3 was used to express VP3 under different conditions. The results demonstrated that most expressed VP3 was in inclusion bodies in all tested conditions. Although …

Evaluation Of Microglia’S Structural Variations Within An Organotypic Hippocampal Slice Model After Regionalized Toxic Injury, Jesus Antonio Trejos

Theses and Dissertations

The dendritic cells of the CNS, the microglia (MG), are an initiation point of the immunological response within the post blood-brain barrier (BBB) compartment. Microglia morphology drastically changes in response to cell stress to a much different non-dendritic morphology. This investigation postulates that if the first MG responses to toxic injury are isolated and studied in greater morphological detail there is much to be learned about MG metamorphosis from a baseline (M2) to an activated (M1) state. The organotypic hippocampal slice was the experimental setting used to investigate microglial response to toxic injury. This isolates dendritic cellular responses and associated …

Interplay Between Rna Editing And Alternative Splicing Modulates Biophysical And Pharmacological Properties Of The Ion Channel Cav1.3, Willy Munyao

Theses and Dissertations

Alternative splicing and adenosine-to-inosine (A-to-I) RNA editing are post-transcriptional mechanisms that promote proteomic diversity in the brain. The impact of RNA editing on brain physiology is underscored by its association with the etiology and pathogenesis of various neurological and neurodegenerative diseases. Alternative splicing of exon 43 within the CaV1.3 C-terminus produces long (CaV1.3L) and short (CaV1.343S) variants exhibiting distinct gating and pharmacological properties. While the physiological role of CaV1.3 A-to-I RNA editing in brain function is still unfolding, recent findings indicate that unedited CaV1.3 channels display larger calcium influx and enhanced neuronal excitability, suggesting that A-to-I RNA editing may act …

Mobocertinib Antagonizes Multidrug Resistance In Abcb1- And Abcg2-Overexpressing Cancer Cells, Xingduo Dong

Theses and Dissertations

The resistance of cancer cells to multiple drugs, known as multidrug resistance (MDR), remains a significant obstacle that leads to the ineffectiveness of cancer chemotherapy. The overexpression of ATP-binding cassette (ABC) transporters, particularly ABCB1 and ABCG2, has been strongly correlated with MDR. There is an urgent need to explore and identify novel inhibitors targeting ABCB1 and ABCG2 to overcome the related MDR. Mobocertinib (TAK-788) is an orally administered, irreversible EGFR/HER2 inhibitor approved for the treatment of adult patients with locally advanced or metastatic non-small cell lung cancer (NSCLC) harboring epidermal growth factor receptor (EGFR) exon 20 insertion mutations. This study …

Combined Cytotoxicity Of The Herbicide Glyphosate And The Fungicide Mancozeb In Mouse Neuroblastoma Cells, Heidi Ebid

Theses and Dissertations

The herbicide glyphosate and the fungicide mancozeb are globally applied on a variety of agricultural crops. Residues of both pesticides are frequently detected in food and water. Combined exposure to both compounds is therefore possible. Glyphosate and mancozeb have been separately investigated in previous studies and were shown to induce neurotoxicity. The current study investigates the cytotoxicity of different glyphosate and mancozeb combinations including mixture of mancozeb + glyphosate, combination of mancozeb followed by glyphosate and combination of glyphosate followed by mancozeb and compares neurotoxicity seen with the combinations to that of glyphosate or mancozeb using mouse neuroblastoma Neuro-2a cells. …

Spatial Variation In Mercury Contamination In Freshwater Fish Species Across Rhode Island, David L. Taylor, Brianna Lotti, Jonathan Serbst, Jim Lake, Alan Libby, Mariel Sorlien

Student Research Symposium

Fish provide essential nutrients and proteins beneficial to human health, yet may pose certain risks due to contaminants in edible tissues. For example, mercury (Hg) is a widespread toxicant that bioaccumulates in fish muscle (filet) tissue. Accordingly, federal and state government agencies issue consumption advisories to minimize diet related Hg exposure. Fish consumption advisories, however, often fail to account for small-scale spatial variations in Hg contamination, especially in freshwater fishes. This study examined site-specific Hg concentrations in bluegill sunfish (Lepomis macrochirus), pumpkinseed sunfish (L. gibbosus), largemouth bass (Micropterus salmoides), chain pickerel (Esox niger), and yellow perch (Perca flavescens), all of …

Maternal Mercury Transfer From Pregnant Spiny (Squalus Acanthias) And Smooth (Mustelus Canis) Dogfish To Their Pups Through Differing Reproductive Strategies, David L. Taylor, Maxwell Ajemian, Abrielle Remick

Student Research Symposium

Mercury (Hg) is a bioaccumulative contaminant that can be transferred from pregnant females to developing embryos (i.e., intrauterine maternal Hg transfer); thus, leading to health deficits in offspring. This study examined Hg transfer in the ovoviviparous spiny dogfish (Squalus acanthias) and viviparous smooth dogfish (Mustelus canis). Female dogfish were collected from Narragansett Bay, Rhode Island Sound, and Block Island Sound via rod & reel, gillnet, and trawling. In the laboratory, intrauterine pups were excised from pregnant dogfish, after which samples of mother and pup muscle tissue were analyzed for total Hg concentrations ([Hg] in ppm dry weight) using automated atomic-absorption …

Mark-Recapture Methods In Eastern Diamondback Rattlesnake (Crotalus Adamanteus) Monitoring, Carissa J. Adams

Theses, Dissertations and Capstones

Eastern diamondback rattlesnakes (Crotalus adamanteus; EDB) are an imperiled species native to the Southeastern United States longleaf pine (Pinus palustris) ecosystem. Several markrecapture methods have been used range-wide in hopes of increasing knowledge on their population numbers. I explored the efficacy of two mark-recapture methods (scale cauterization and dorsal head photography) using the Marine Corps Recruit Depot, Parris Island, SC EDB population in this multi-part study. The first method I evaluated was ventral scale branding, which has been widely implemented without knowledge of brand permanence. I classified misidentification errors at capture status (new versus recapture) and …

Factors Determining The Success Of Recently Planted Spartina Alterniflora On A Living Shoreline In Jamaica Bay, Ny, Abby K. Bressette, Shakira Thomas, Brett F. Branco

Environmental Studies Publications

Natural and nature-based features have been implemented throughout New York City to provide habitat and reduce flooding, erosion, and pollution. The West Pond Living Shoreline in Jamaica Bay, NY was designed with organic breakwaters and native marsh plants, including S. alterniflora. Post-construction monitoring in 2021 found that percent coverage of S. alterniflora was lower than expected. To inform future management, we studied the effect of elevation, planting method, and shoreline energy exposure on the percent cover, average stem height, and average stem density of S. alterniflora. Elevation and plant growth data were collected within 8 one sq. meter quadrats at …

Assessing The Role Of Kmt2b Knockdown In Rhabdoid Tumorigenesis Utilizing Crispri, Karah Edmonds, Andrea Florian Phd

Science University Research Symposium (SURS)

An aggressive blood cancer commonly observed in pediatric patients, Mixed Lineage Leukemia (MLL), has a regulatory MLL complex that promotes histone methyltransferation. Modifying histones utilizing histone methyltransferases like KMT2B (MLL2), is essential for gene expression by regulating transcription. Loss of function and inhibition of KMT2B leads to dysregulation of transcription and therefore promotes MLL tumorigenesis. To improve cancer-targeted therapies, we will use CRISPR interference (CRISPRi) to knock down the expression of KMT2B and measure levels of Rhabdoid tumor cell proliferation and apoptosis. Previous research surrounding the MLL complex suggests that decreased expression of KMT2B will lead to increased levels of …

Ms Environmental Biology Capstone Project, Reilly Miller

Regis University Student Publications (comprehensive collection)

Habitat loss due to deforestation is a primary threat to global biodiversity. Clearing tropical rainforests for agriculture leads to forest fragmentation. Forest fragments contain fewer large trees and provide lower food availability for primates compared to continuous forests. Mantled howler monkeys inhabit the increasingly fragmented rainforests of Central and South America and may need to alter their activity and spatial cohesion to mitigate competition and preserve energy in fragments where there is lower quality food. We compared howler monkey activity and spatial cohesion across a small forest fragment (La Suerte Biological Research Station, LSBRS) and a large, continuous forest (La …

Evaluation Of Hormonal Suppression Treatments Of Endometriosis, Olivia Vermeulen

Biology and Microbiology Graduate Students Plan B Research Projects

Endometriosis is a chronic, multifactorial, and estrogen dependent disease that causes lesions made of endometrial tissue to implant in areas other than the uterus such as the ovaries, fallopian tubes, abdominal cavity, lungs, and even heart (1). These lesions can cause an immense amount of pain in the implanted area during the menstrual cycles and can cause infertility issues, but unfortunately there are not many available and effective treatment options available that do not involve surgically removing the lesions (2). However, there are certain hormonal suppression medications being that are new or are being tested that can alleviate inflammation and …

Epigenetics And Character Virtue Development, David S. Moore

Pitzer Faculty Publications and Research

Current understandings of epigenetics offer some insights into the developmental origins of character virtues. Research on epigenetics has demonstrated that phenotypes depend on both genomic and non-genomic information for their development, and such is true for the development of character virtues as well. This conclusion offers significant support for the Developmental Systems perspective. Although researchers have not yet studied the role of molecular epigenetic factors in the development of character, the field of behavioral epigenetics has revealed how such factors contribute to psychological and other behavioral phenotypes in general. In particular, behavioral epigenetics research can highlight the plasticity of human …

Older Adult Compendium Of Physical Activities: Energy Costs Of Human Activities In Adults Aged 60 And Older, Erik A. Willis, Stephen D. Herrmann, Mary Hastert, Chelsea L. Kracht, Tiago V. Barreira, John M. Schuna Jr., Zhenghua Cai, Minghui Quan, Scott A. Conger, Wendy J. Brown, Barbara E. Ainsworth

Kinesiology Faculty Publications and Presentations

Purpose: To describe the development of a Compendium for estimating the energy costs of activities in adults ≥60 years (OA Compendium).

Methods: Physical activities (PAs) and their metabolic equivalent of task (MET) values were obtained from a systematic search of studies published in 4 sport and exercise databases (PubMed, Embase, SPORTDiscus (EBSCOhost), and Scopus) and a review of articles included in the 2011 Adult Compendium that measured PA in older adults. MET values were computed as the oxygen cost (VO₂, mL/kg/min) during PA divided by 2.7 mL/kg/min (MET₆₀₊) to account for the lower resting metabolic rate …

Role Of Plasmapheresis: A Tale Of Two Thyroid Storms, Zhiwei Zhang, Sam Joseph, Donald Eagerton

South Atlantic Division GME Research Day 2024

No abstract provided.

2024 Annual Report, Tennessee. Division Of Forestry And Tennessee. Department Of Agriculture

Forestry Annual Reports

This annual report presents an overview of Tennessee Forestry Commission programs and management.

Unveiling The Past: A Comparative Demographic Analysis Of The Black Population In Columbus, Georgia With Other Contemporaneous Data (1700'S To Present), Torie Hartis, Michael Newbrey, Jennifer Newbrey

CSU Tower Day

No abstract provided.

Two New Species Of Dulcicalothrix (Nostocales, Cyanobacteria) From India And Erection Of Brunnivagina Gen. Nov., With Observations On The Problem Of Using Multiple Ribosomal Operons In Cyanobacterial Taxonomy, Aniket Saraf, Prashant Singh, Naresh Kumar, Sagarika Paul, Jeffrey R. Johansen

2024 Faculty Bibliography

Two new species of Dulcicalothrix, D.adhikaryi sp. nov. and D.iyengarii sp. nov., were discovered in India and are characterized and described in accord- ance with the rules of the International Code of Nomenclature for algae, fungi, and plants (ICN). As a result of phylogenetic analysis, Calothrix elsteri is reas- signed to Brunnivagina gen. nov. During comparison with all Dulcicalothrix for which sequence data were available, we observed that the genus has six ribosomal operons in three orthologous types. Each of the three orthologs could be identified based upon indels occurring in the D1–D1′ helix sequence in the ITS rRNA region …

Four Novel Species Of Kastovskya (Coleofasciculaceae, Cyanobacteriota) From Three Continents With A Taxonomic Revision Of Symplocastrum, Brian Jusko, Jeffrey R. Johansen, Smail Mehda, Elvira Perona, M Angeles Muñoz-Martín

2024 Faculty Bibliography

Studies performed in North America, Africa, and South America have led to the isolation of four new species of Kastovskya, a filamentous cyanobacterial genus that before this manuscript had only one species, Kastovskya adunca from Chile. Kastovskya nitens and K. viridissima were isolated from soils on San Nicolas Island, K. sahariensis was isolated from hypolithic habitats from the Sahara Desert in Algeria, and K. circularithylacoides was isolated from hypolithic habitats in Chile. The molecular analyses are corroborated by morphological data, morphometric analysis, and ecological and biogeographical considerations for robust polyphasic descriptions of all taxa. The peculiar transatlantic distribution of this …

A Multivariate Analysis Of Phytoplankton Assemblages And Environmental Variables In Chautauqua Lake, New York, Emily Palmer

Masters Theses

No abstract provided.

Adolescent Mental Health In The Twenty-First Century, Arthur Maerlander, Patricia Gail Bray

Center for Brain, Biology, and Behavior: Faculty Publications

Conclusion

Parents do not suddenly relinquish medical decision-making to their children and are a vital part of a process construed within the framework of consent and confidentiality laws. In the transition period from childhood to adulthood, adolescents develop unique healthcare needs and concerns at the very time they are gaining autonomy from their parents. Adolescents progress from being recipients of decisions about their care to gradually becoming active participants in their healthcare and emerge as a primary partner in medical decision-making. Building a strong therapeutic alliance with young people can help them become responsible healthcare decision-makers.

Yet while “healthcare,” including …

Associations Of Sex And Sport Contact‑Level With Recovery Timelines Among Collegiate Athletes With Sport‑Related Concussion, Bernadette A. D'Alonzo, Andrea L. C. Schneider, Ian J. Barnett, Christina L. Master, Abigail C. Bretzin, Douglas J. Wiebe, Ivy League-Big Ten Epidemiology Of Concussion Study Investigators

Center for Brain, Biology, and Behavior: Faculty Publications

Background

Growing interest has motivated recent studies to examine differences in recovery after sportrelated concussion (SRC) by sex. However, heterogeneity in study design, participants, and recovery outcomes has led to mixed findings. Further work is needed to evaluate potential differences by sex and to investigate the role of related characteristics, such as sport contact-level, in recovery timelines. This study aimed to investigate whether concussion recovery trajectories differ by sex, considering a priori clinical and demographic covariates, and accounting for the sequence of recovery outcomes. Our secondary question was whether sport contact-level modifies the relationship between sex and time to outcomes. …

Toolkit To Address Free-Ranging Domestic Cats On Agency Lands Managed For Native Wildlife And Ecosystem Health, Sara H. Schweitzer, Colin M. Gillin

Human–Wildlife Interactions Monographs

Native flora and fauna of North America face numerous threats, and impacts from invasive species are some of the most challenging. Free-ranging domestic cats (Felis catus) are extremely harmful to wildlife and the environment. The Association of Fish and Wildlife Agencies (AFWA) developed a toolkit to provide guidance to fish and wildlife agencies as they address free-ranging domestic cats on agency lands managed for native wildlife and ecosystem health. Topics in this document include wildlife conservation, infectious diseases, legal issues, education and outreach, human dimensions, partnerships, management strategies, and model regulatory and legal language. This toolkit is not …

Empowering Kokal: A Decolonized, Community-Led Development Model In Homa Bay County, Kenya, Kennedy Miruka

Regis University Student Publications (comprehensive collection)

In Homa Bay County, Kenya, a community-led development initiative called the Power Centre is transforming the fight against poverty and powerlessness. Grounded in principles of decolonization and participatory action, this project addresses the root causes of marginalization by empowering residents of Kokal Village to become active agents of change. Through a robust mixed-methods research approach, the project identifies systemic challenges and leverages existing social networks to build a sustainable, locally-owned model for development. The Power Centre implements a three-pronged strategy, focusing on increasing community participation, strengthening existing groups, and fostering collaborative networks to weave a robust support system. By addressing …