Life Sciences Commons^™

Lipid Genetics In Pregnant Women: Maneuvering The Challenges Of Using Degraded Samples, Kylee Meece

Mahurin Honors College Capstone Experience/Thesis Projects

The results of this project will explore a novel approach to lipid metabolism in pregnant women by exploring the relationship between maternal gene expression of genes critical for fat metabolism, protein expression, and maternal and neonatal outcomes. The study will also examine gene expression in lean pregnant women compared to obese pregnant women, shedding light into the adverse pathology of obesity during pregnancy. These findings may uncover a mechanism related to gene-expression that connects unfavorable maternal fat metabolism to inflammation and/or insulin resistance, which may help to uncover future targets for interventions.

The analysis of lipid gene expression requires the …

Prevalence Of Insecticide Resistance Gene In Aedes Albopictus Population In Illinois Regions, Merylann Ochieng

2024 Awards for Excellence in Student Research and Creative Activity - Documents

Mosquitoes are vectors known to transmit many pathogens and parasites that cause serious human diseases such as Malaria, Dengue, Yellow and Chikungunya fever, Encephalitis, and Filariasis (Becker et al., 2010) (Franklinos et al., 2019). Aedes albopictus specie transmits Zika, Chikungunya, yellow Fever and Rift valley fever viruses (Leta et al., 2018). Aedes species are geographically invading new areas due to globalization and environmental changes (Lwande et al., 2020). Temperature increase, severe rainfall, and high humidity encourage diseases to spread to new areas where new vectors may act as carriers (Näslund et al., 2021). Aedes albopictus are expanding further along the …

The Impact Of Fish Intake (Primarily The Omega-3 Fatty Acid Concentration) On Cognitive Decline: A Systematic Review, Emma Martin

2024 Awards for Excellence in Student Research and Creative Activity - Documents

The purpose of this systematic review is to explore the effect of fish intake on cognitive decline. Previous studies on fish intake and cognitive decline have produced both supportive results and results that yield ineffectiveness. This review focuses primarily on omega 3 concentration from fish intake and its influence on mild cognitive impairment. Using several search engines, studies were selected by searching “fish intake and cognitive decline”. Inclusion criteria consisted of individuals diagnosed with MCI, intervention of fish intake, and omega 3 fatty acid concentration and cognitive function exams conducted at baseline and throughout the study. Nine out of the …

Thymidine Phosphorylase Is A Promising Target In Sars-Cov-2 Spike Protein-Enhanced Thrombosis, Renat Roytenberg

Theses, Dissertations and Capstones

Thrombosis, as an underlying mechanism in cardiovascular diseases, is the leading global cause of death in the 21st century. Infection is a risk factor for thrombosis, and the coronavirus disease 2019 (COVID-19) pandemic has led to a rise in the incidence of thrombosis, thereby increasing mortality. In comparison to previous severe coronavirus outbreaks, rates of thrombosis in COVID-19 have been unprecedented. COVID-19 is caused by the novel coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The SARS-CoV-2 spike protein (SP), the key used to enter host cells, is implicated in thrombosis pathology, though mechanisms are not fully understood. The SARS-CoV-2 …

Differential Tail Autotomy Between Two Genetic Clades Of A Woodland Salamander, Plethodon Cinereus, Ryan Mayer

Masters Theses

Following the retreat of glaciers in North America, a wide diversity of organisms rapidly migrated into previously uninhabited regions, including animals with limited dispersal abilities such as amphibians. I wanted to better understand the strategies that salamanders use to aid in their dispersal abilities and to understand if patterns of post-glacial range expansion affect phenotypic traits such as anti-predator strategies. In this study, I used existing phylogeographic evidence of clade membership as a framework to examine whether red-backed salamanders (Plethodon cinereus) differed in tail autotomy behavior at the range edge versus the range core, and across the geographic range of …

Examining Quantitative Honest Signaling In Aposematic Traits Of The Green And Black Poison Frog Dendrobates Auratus From Costa Rica, Katherine Porras-Brenes

Masters Theses

No abstract provided.

A Global Synthesis Of The Relative Impacts Of Habitat Amount, Fragmentation, And Matrix Quality On Forest Biodiversity, Antoinette Esposito

Masters Theses

Aim To assess the relative impact of different landscape variables on species richness and to determine whether species richness declines more rapidly below an extinction threshold of remaining habitat. The results of this study will help to better inform future conservation strategies. Location Global Time period 1997 – 2013 Major taxa studied Amphibians, birds, invertebrates, mammals, and reptiles Methods Data from 71 studies published in the global BioFrag database were used to determine species richness across multiple landscapes and biomes. The Hansen dataset was used to collect data on habitat amount (forest area), fragmentation (patch density), and matrix quality (mean …

Molecular Identification To Verify The Existence Of Helicotylenchus Dihystera In Coffee Plants At East Java, Indonesia, Ankardiansyah Pandu Pradana, Toyoshi Yoshiga

ASEAN Journal on Science and Technology for Development

Spiral nematodes Helicotylenchus sp. are known to infect a variety of plants, including coffee. This is in line with the studies and surveys of nematodes in Indonesia. However, to date, there has been no study reporting the nematode with molecular analysis. In this study, we surveyed the nematodes in Arabica coffee plantations in Bondowoso regency – Indonesia. These spiral nematodes were collected in DESS solution for morphological, morphometric and molecular analysis. The morphometric analysis was carried out under an Olympus BH-2 compound microscope with an attached calibrated Toupcam M20 camera. The molecular analysis was performed on the total DNA from …

Species Distribution Modelling Using Bioclimatic Variables On Critically Endangered Endemic Species (Macrocephalon Maleo) In Sulawesi, Septianto Aldiansyah, Khalil Abdul Wahid

ASEAN Journal on Science and Technology for Development

The island of Sulawesi isolates migratory fauna from Asia and Australia and creates animal combinations such as the Maleo (Macrocephalon Maleo). The Maleo population is experiencing a downward trend due to habitat fragmentation, deforestation and threats by humans. This study aims to model the potential distribution of Maleo using several famous current and future models as a result of climate change throughout the island of Sulawesi, not only in its natural habitat, but beyond its natural habitat. Bioclimatic variables and in situ attendance data were used in this study. The method used is Maximum Entropy by evaluating the GLM, SVM …

The Musical Descriptor Using Fast Fourier Transform (Fft): Examples In P Ramlee Songs, Ahmad Faudzi Musib, Sinin Hamdan, Kuryati Kipli, Aaliyawani Ezzerin Sinin, Johari Abdullah

ASEAN Journal on Science and Technology for Development

The musical descriptor can be characterize using quantitative evaluation of audio sounds. This paper evaluates the musical descriptors for P Ramlee instrumental songs utilizing the Fast Fourier Transform (FFT) from the audio sounds signal. In this study, Python programming language is used to develop a series of custom scripts to calculate the musical descriptors. The approach involved constructing musical descriptors from instrumental music through analysis and digital processing of the spectra. The signal data were first transformed using the FFT to extract frequencies and intensities, which are crucial for identifying the spectral signature of musical sounds. The FFT is particularly …

Development Of A Novel Edna Sampling Method For Macroinvertebrate Species Of Greatest Conservation Need (Sgcn) In Texas, James York

Biology Theses

Environmental DNA (eDNA) sampling is a new sampling technique that allows for non-invasive sampling of target species or the characterization of entire communities based on barcoding genes. The goal of this study was to sequence common barcoding genes such as 28S rRNA and Cytochrome C Oxidase I (COI), and then develop species-specific primers for eight macroinvertebrates listed as Species of Greatest Conservation Need (SGCN) in Texas. After sampling for the target species, five were not found, all of which were in the Order Trichoptera (caddisflies). A sixth target species, Isoperla sagittata, was found to have only one gap and …

Investigating Scube3 Nuclear Localization In Presence Of Doxorubicin Treatment In Triple Negative Breast Cancer Cells, Lillian Ergle

Merge

Signal peptide-CUB-EGF-like domain-containing protein 3 (SCUBE3) is a glycosylated secreted protein. Prior to its secretion, SCUBE3 localizes in the cytoplasm. We observed that SCUBE3 protein trans-localized to the nucleus following Doxorubicin (DOX) treatment. DOX is one of the strongest chemotherapeutic agents and the first-line drug used in breast cancer treatment. SCUBE3 structural analysis showed that it lacks a DNA binding domain. Based on this observation and other preliminary data, we hypothesized that nuclear SCUBE3 protein promotes the survival of cells against Doxorubicin treatment. To investigate this hypothesis, we made a wild-type construct and three constructs with mutated nuclear localization sequences …

Documentation Of Hadrosaurid Humeri From The Liscomb Bonebed, North Slope, Alaska Prior To Histological Sectioning, Makenna Galvin

Student Research Poster Presentations 2024

Proper documentation of research specimens is a crucial component that must be completed prior to histological sectioning. Since specimens are unique and irreplaceable, documentation provides information that may later be unobtainable after sectioning, due to the inherent nature of the technique having to permanently alter the specimen. The hadrosaurid dinosaur, the Edmontosaurus, has an abundant fossil record, which can be partly attributed to being gregarious, and therefore preserves multiple monodominant bonebeds and several dozen associated skeletons across North America. This makes Edmontosaurus an ideal taxon to use for life history studies. However, previous Edmontosaurus bonebeds have only preserved specimens of …

The Effects Of Bisphenol S On Glial Cells Of The Peripheral Nervous System, Madeline Solomon, Rachael Sennett, Angela Asirvatham

Student Research Poster Presentations 2024

Schwann cells are glial cells in the peripheral nervous system that maintain nerve function and myelination. Peripheral nerve myelination is dependent on intact Schwann cell proliferation. Schwann cell proliferation can be adversely affected by environmental pollutants such as Bisphenol A (BPA). BPA is an endocrine-disrupting chemical found in plastic water bottles, receipt paper, containers, and many everyday products. Studies on BPA exposure showed that it stimulated cellular proliferation in neural stem cells. A commonly used manufactured derivative of BPA, Bisphenol S (BPS), has posed similar concerns. Previous research has found that BPS promotes the proliferation of MCF-7 breast cancer cells …

The Effects Of Bpf On Rt4 Schwannoma Cancer Cells, Rachael Sennett, Madeline Solomon, Angela Asirvatham

Student Research Poster Presentations 2024

Bisphenol A is a harmful compound common in plastics that causes negative health and environmental effects. Due to these effects, new guidelines have been made to limit exposure to BPA. However, modified versions of these compounds, including Bisphenol-F, are being used as a replacement. Exposure to BPF, which is comparable in structure to BPA, has similar effects as BPA on many organ systems with some being unknown. As there is not much known about BPF, especially in the peripheral nervous system, the aim of this research is to analyze proliferation rates in BPF-treated Schwann cells. It was hypothesized that with …

Nutritional Convergence In Plants Growing On Gypsum Soils In Two Distinct Climatic Regions, Clare T. Muller, Andreu Cera, Sara Palacio, Michael J. Moore, Pablo Tejero, Juan F. Mota, Rebecca E. Drenovsky

2024 Faculty Bibliography

Background and Aims

Soil endemics have long fascinated botanists owing to the insights they can provide about plant ecology and evolution. Often, these species have unique foliar nutrient composition patterns that reflect potential physiological adaptations to these harsh soil types. However, understanding global nutritional patterns to unique soil types can be complicated by the influence of recent and ancient evolutionary events. Our goal was to understand whether plant specialization to unique soils is a stronger determinant of nutrient composition of plants than climate or evolutionary constraints. Methods

We worked on gypsum soils. We analysed whole-plant nutrient composition (leaves, stems, coarse …

Description Of Six New Cyanobacterial Species From Soil Biocrusts On San Nicolas Island, California, In Three Genera Previously Restricted To Brazil, Brian Jusko, Jeffrey R. Johansen

2024 Faculty Bibliography

As the taxonomic knowledge of cyanobacteria from terrestrial environments increases, it remains important to analyze biodiversity in areas that have been understudied to fully understand global and endemic diversity. This study was completed as part of a larger algal biodiversity study of the soil biocrusts of San Nicholas Island, California, USA. Among the taxa isolated were several new species in three genera (Atlanticothrix, Pycnacronema, and Konicacronema) which were described from, and previously restricted to, Brazil. New taxa are described herein using a polyphasic approach to cyanobacterial taxonomy that considers morphological, molecular, ecological, and biogeographical factors. Morphological data corroborated by molecular …

The Impact Of Isolation From Other Members Of The Greater Bamboo Lemur (Prolemur Simus) Species On The Behavior Of A Lone Greater Bamboo Lemur In Ranomafana National Park, Marissa N. Mccandless, Alex Casamassima, Sophie Thomas

Undergraduate Research (Journal)

Across Madagascar, many lemur species are declining in number due to deforestation perpetuated by slash and burn agriculture. Ranomafana National Park hosts 12 unique lemur species, one of which has a local population of just a single individual- the Greater Bamboo lemur (Prolemur simus). Being extremely social beings, we theorize that isolation from members of one’s species will change behavioral patterns. To test this hypothesis, we used focal animal sampling to record the behavior of the Greater Bamboo lemur for about 40 hours over a five day period. We compared our results to behavioral data collected of lemurs in a …

Precommentary: Animal Personality Needs Animal Sentience, Ralph Adolphs, Yue Xu

Animal Sentience

We are grateful to Owens, Bryja & Bekoff (2024) for their important discussion of individual differences in animals, emphasizing the role of personality in conservation, wildlife research, and wellbeing. But their emphasis also raises new challenges: How should we conceive of personality in nonhuman species? what modern tools could be leveraged to best measure it? and, perhaps most important of all: how can we ensure that the conscious experience of animals — their capacity for wellbeing and for suffering — is not forgotten along the way? We touch on each of these challenges in this invited Precommentary in the hope …

Complexities Of Consistent Individual Behavioral Differences In Effective Wildlife Management, Nathalie R. Sommer

Animal Sentience

The study of animal personality, or consistent individual behavioral differences, has faced numerous challenges since its inception, including terminological disputes, labor-intensive methodologies, and notable retractions. This commentary explores the mechanisms behind personality traits, questioning the link between personality and sentience, and highlights the bias towards megafauna in wildlife management. It underscores the ecological and ethical significance of considering arthropod personalities and the complexities of integrating personality into wildlife reintroductions. Caution is advised in generalizing personality effects, and the limitations of current measurement techniques are discussed, suggesting that personality traits should be one of many factors in comprehensive management strategies.

Individuality Matters But Its Cause Has Consequences, Daniel T. Blumstein

Animal Sentience

I discuss the relationship between individuality and heritability and note that the mechanism driving individual differences matters for conservation and management outcomes.

A Comparative Study Of Biocrusts On Gypsum And Non-Gypsum Soils In The Northern Chihuahuan And Eastern Mojave Deserts, Usa: Biocrust Mosses Respond To Soil, Environmental, And Climatic Conditions, Katelyn Gobbie

Masters Theses

Biological soil crust communities (biocrusts) growing on gypsum soils have been well- documented for their prolific appearance and rich diversity of lichens and bryophytes. However, studies characterizing gypsum biocrusts have primarily occurred outside of the U.S., most of which lack comparisons to other soil types. We conducted intensive field surveys to evaluate the cover and frequency of biocrust functional groups and moss species on gypsum and non-gypsum soils in the U.S. regions with the most extensive gypsum outcrops, the northern Chihuahuan and eastern Mojave Deserts. We employed canonical correspondence analysis (CCA) to relate the observed differences in biocrust abundance and …

Nurse's Knowledge On Mind Diet For Early-Onset Alzheimer's Disease, Audrey Marie Rafols

Nursing | Student Research Posters

Adults with Early Onset Alzheimer's Disease (EOAD), is the less common form of the neurodegenerative disease of Alzheimer's Disease (AD), where EOAD is found in adults younger than 65 years old (Alzheimer's Association, 2024). Characterized by a decline of memory, cognitive decline, loss of ability to perform daily tasks involving mobility and communication, EOAD does not have one significant cause. A combination of risk factors are genetics and lifestyle factors such as heart disease, history of stroke, and high blood pressure are associated with increasing the risk of EOAD (National Institute of Aging, 2024).

Since there is no cure for …

Progression Magazine, 2023-2024, Coastal Carolina University

Progression Magazine

Magazine of the Gupta College of Science at Coastal Carolina University.

A Diagnostic Challenge – Tinu Syndrome, Sophia Lee, Md, Claire Kassakian, Md

Poster Presentations

No abstract provided.

Efficacy Of Cloves As A Repellent For Pest Ants (Formicidae), Aadee Malavade

2024 REYES Proceedings

I examined the efficacy of cloves for repellent activity against foraging ants. Control baits consisting of a cracker/paper substrate topped with granulated sugar or chapati (Indian bread) were presented next to treatment baits (cracker/paper, granulated sugar and spice spread over the sugar) to ants in natural habitats. After an hour, the numbers of ants recruiting to the control and treatment baits were recorded. A timed study of ants with 30-minute duration was done to understand behavior of ants over time. Data were analyzed with the sign test. Cloves showed promise as ant repellents.

Ants And Their Response To Chili Powder, Antu Biswas

2024 REYES Proceedings

Spices can be used as an eco-friendly ant repellent. In this study, I tried to observe the ants’ response to chili powder and whether or not the spice can be an effective ant repellent.

The Efficacy Of Dry Peppermint Leaves As An Ant Repellent, Amal Chacko

2024 REYES Proceedings

Ant (Formicidae) infestation is a typical residential disturbance, often necessitating the use of chemical repellents that pose environmental and health dangers. This study looks into the possibilities of a natural alternative, dry peppermint (Mentha piperita L) leaves as a chemical repellent. Given the diversity of ant species and their variable responses to stimuli, this study seeks to determine the efficiency of dried peppermint leaves as a general ant repellent. A pilot study will be carried out to determine the repellent qualities of this spice against a specific ant species. The precise aims include evaluating if dry peppermint leaves …

Pharmacokinetic Modeling Of Metonitazene And Isotonitazene Using In Vitro And In Vivo Models And Comparison To Human Postmortem Casework, Justine P. Sorrentino

Theses and Dissertations

Metonitazene and isotonitazene are two benzimidazole opioids that are contributing to the opioid crisis nationwide. Limited pharmacokinetic data has been published on these nitazene compounds, therefore this study sought to determine if these two structurally similar opioids would have comparable half-lives and clearance utilizing in vitro and in vivo models. Using the data determined in the pharmacokinetic study, and by testing human postmortem cases, this study aimed to inform interpretation of these drugs detected in human casework. Sample analysis for all aspects of the study was performed utilizing LC/MS/MS. In vitro pharmacokinetics of both drugs were examined using a human …

Streptozotocin-Induced Diabetes Increases Kidney Toxicity In Rats Exposed To Ochratoxin A, Belkys Gonzalez

Theses and Dissertations

Due to the rising occurrence of diabetes in the population and the prevalence of OTA exposure via ingestion of contaminated foodstuffs, there is a need to assess the possible renal interaction of a diabetic disease state in concurrence with OTA exposure. Sprague Dawley rats were treated with streptozotocin to induce experimental diabetes and then orally exposed to 0.5 mg/kg body weight OTA once a day for 21 days. Renal biomarkers and light microscopy were evaluated to assess the interaction between diabetic nephropathy and OTA exposure. LLC-PK1 proximal tubule cells were used as an in vitro model to assess the potential …