Life Sciences Commons^™

Using Recurrent Phenotypical Selection To Improve Drought Tolerance Of Red Clover, M. Sahin, S. Reid, E. Sahin, S. Ates, S. Elias, David B. Hannaway

IGC Proceedings (1977-2023)

Red clover is one of the most important forage legumes in the world. It is utilized extensively in European and North American farming systems. Approximately, 4 million hectares of red clover are grown globally each year for forage production, soil improvement, and as a pollen and nectar source for pollinators (Riday, 2010). In North America, red clover is widely used in temperate regions for pasture, hay, and silage in combination with grasses for soil improvement and as a pollen and nectar source for bumblebees (Bombus). In the northwest, it is also grown for seed production (Anderson et al., 2016) with …

Legumes As A Biological Tool To Address The Sustainability Of Ruminant Production Systems, H. Sheridan, M. B. Lynch

IGC Proceedings (1977-2023)

Growing public concern regarding accelerated rates of climate change, the depletion and degradation of natural resources such as biodiversity water and soils, coupled with policy commitments to address these challenges, are placing increasing pressures to enhance sustainability metrics associated with agriculture in general, and ruminant production systems in particular. At EU and indeed global scale, there has probably never before been so many potentially conflicting challenges for agriculture. On one hand, agricultural systems need to produce more, to feed the increasing global human population, while at the same time being much less reliant on economically and environmentally costly chemical inputs …

How Do Artemisia Capillaris Population Respond To Grazing Management And Rain Reduction?, Li-Yuan Shi, Fu-Jiang Hou, Zhen Wang

IGC Proceedings (1977-2023)

Climate and human activities, such as drought event and livestock grazing, generally coupled to influence the structure and function of grassland ecosystems. However, most of previous studies focused on the isolated effects of grazing or drought on grassland ecosystems, with little attention paid to the combined effects of them. Further, we know little about how plants respond to grazing and drought at the population level. We conducted a grazing regime (enclosure, stop grazing, and heavy grazing) and drought manipulation experiment in a typical steppe to explore how grassland plants response to ongoing drought and grazing regimes at the population level. …

Development Of Forage Curriculum For Extension Educators In The Southeast Usa, L. S. Silva, L. Dillard, K. Mullenix, M. Wallau, J. J. Tucker, C. Vasco

IGC Proceedings (1977-2023)

In the Southeast USA, livestock production is one of the largest agricultural activities, and forages are the primary feed source. Most livestock systems are highly dependent on off-farm inputs to support forage production and animal performance, which elevates production costs and the activity's carbon footprint. There is a strong need to develop forage educational resources to enhance productivity, environmental sustainability, resilience, and profitability of agricultural systems in the region. This multi-disciplinary initiative was a collaboration among several land-grant Universities across the region. The objective was to develop decision tools and provide in-classroom training associated with hands-on demonstrations to Extension agents …

Steminism: Analyzing Factors That Improve Retention Of Women In Stem, Kira Carter, Jane Kelley, Jason Vasser-Elong, Rc Patterson

Dissertations

Our co-authored research ‘Steminism: Analyzing Factors That Improve Retention for Women as STEM Majors’ analyzed factors that contributed to the retention of women in science, technology, engineering, and mathematics (STEM) programs at Missouri University of Science & Technology (Missouri S&T). Women make up half of the US population, and while careers in (STEM) are an integral part of the US economy, women are underrepresented in these career fields. The purpose of our dissertation is to address the underrepresentation of women in STEM majors. Our methodology included homogeneous sampling to collect qualitative data. More specifically, we consulted with academic advisors and …

A Spatio-Temporal Modelling Approach To Understand The Effect Of Urban Fruit Fly Outbreaks On Peri-Urban Orchards, Andrew Broadley, Rieks Van Klinken, Dean R. Paini, Matthew Hill, Elliot T. Howse

Horticulture Research Articles

Urban areas are well-known sources of fruit fly introduction and unmanaged populations pose a high risk of dispersal to surrounding agricultural areas. Although management of urban fruit fly populations is recognised as being important for area-wide control programmes, its relative importance is rarely quantified. Identifying possible sources of fruit fly populations is important to the success of area-wide programs, as well as to on-farm management. We developed a modelling approach to analyse the spatio-temporal connectivity of fruit fly across urban-agriculture boundaries. For a case study, we examine Mediterranean fruit fly, Ceratitis capitata, outbreaks in two fruit growing …

Tailoring T Fh Profiles Enhances Antibody Persistence To A Clade C Hiv-1 Vaccine In Rhesus Macaques, Anil Verma, Chase E. Hawes, Sonny R. Elizaldi, Justin C. Smith, Dhivyaa Rajasundaram, Gabriel Kristian Pedersen, Xiaoying Shen, La Tonya D. Williams, Georgia D. Tomaras, Pamela A. Kozlowski, Rama R. Amara, Smita S. Iyer

School of Medicine Faculty Publications

CD4 T follicular helper cells (Tfh) are essential for establishing serological memory and have distinct helper attributes that impact both the quantity and quality of the antibody response. Insights into Tfh subsets that promote antibody persistence and functional capacity can critically inform vaccine design. Based on the Tfh profiles evoked by the live attenuated measles virus vaccine, renowned for its ability to establish durable humoral immunity, we investigated the potential of a Tfh1/17 recall response during the boost phase to enhance persistence of HIV-1 Envelope (Env) antibodies in rhesus macaques. Using a DNA-prime encoding gp160 antigen and Tfh polarizing cytokines …

Immunotherapy Resistance In Solid Tumors: Mechanisms And Potential Solutions, Daniel Lefler, Steven Manobianco, Babar Bashir

Kimmel Cancer Center Faculty Papers

While the emergence of immunotherapies has fundamentally altered the management of solid tumors, cancers exploit many complex biological mechanisms that result in resistance to these agents. These encompass a broad range of cellular activities - from modification of traditional paradigms of immunity via antigen presentation and immunoregulation to metabolic modifications and manipulation of the tumor microenvironment. Intervening on these intricate processes may provide clinical benefit in patients with solid tumors by overcoming resistance to immunotherapies, which is why it has become an area of tremendous research interest with practice-changing implications. This review details the major ways cancers avoid both natural …

A Longitudinal Look At Student Attitude, Perceived Competence, And Fitness Test Performance Of Elementary Students, Risto Marttinen, Kevin Mercier, Jung Yeon Park, Kelly L. Simonton, Erin Centeio, Kevin Andrew Richards, Kathleen Wilson

International Journal of Physical Activity and Health

The purpose of this study was to assess relationships between students’ attitudes toward physical education (PE), their perceived competence toward PE, and their fitness test performance, as well as how these relationships change over time. Measurements were conducted with validated instruments across five time points during two school years with fourth and fifth grade students across ten schools (n = 636, 48% = female). This study was developed to address a need for examining how attitudes and perceived competence change over time. Descriptive statistics and latent growth models were run with the variables of interest to investigate four research …

Smokejumper Obituary: Johnshoy, Glen A. (Missoula 1967), National Smokejumper Association

Smokejumper Obituaries

No abstract provided.

Application Of Dna Barcodes In The Genetic Diversity Of Hard Ticks (Acari: Ixodidae) In Kazakhstan, Ziwei Zheng, Weixin Zeng, Suwen Wang, Wenbo Tan, Xiaobo Lu, Kenesbay Kairullayev, Ligu Mi, Wurelihazi Hazihan, Gang Liu, Meihua Yang, Yuanzhi Wang

Harold W. Manter Laboratory of Parasitology: Library Materials

Forty-five tick species have been recorded in Kazakhstan. However, their genetic diversity and evolutionary relationships, particularly when compared to ticks in neighbouring countries, remain unclear. In the present study, 148 mitochondrial cytochrome c oxidase subunit I (COI) sequence data from our laboratory and NCBI (National Center for Biotechnology Information; https://www.ncbi.nlm.nih.gov/) data were used to address this knowledge gap. Phylogenetic analyses showed that i) Hyalomma anatolicum anatolicum (Koch, 1844) ticks from Jambyl Oblast (southeastern Kazakhstan) and Gansu Province (northwestern China) constituted a newly deviated clade; and ii) Dermacentor reticulatus (Fabricius, 1974) ticks from South Kazakhstan Oblast were closer …

Establishment And Characterization Of Turtle Liver Organoids Provides A Potential Model To Decode Their Unique Adaptations, Christopher Zdyrski, Vojtech Gabriel, Thea B. Gessler, Abigail Ralston, Itzel Sifuentes-Romero, Debosmita Kundu, Sydney Honold, Hannah Wickham, Marco Arriaga, Jacob Galan

School of Medicine Publications

Painted turtles are remarkable for their freeze tolerance and supercooling ability along with their associated resilience to hypoxia/anoxia and oxidative stress, rendering them an ideal biomedical model for hypoxia-induced injuries (including strokes), tissue cooling during surgeries, and organ cryopreservation. Yet, such research is hindered by their seasonal reproduction and slow maturation. Here we developed and characterized adult stem cell-derived turtle liver organoids (3D self-assembled in vitro structures) from painted, snapping, and spiny softshell turtles spanning ~175My of evolution, with a subset cryopreserved. This development is, to the best of our knowledge, a first for this vertebrate Order, and complements the …

Estrogen Receptor Mutations As Novel Targets For Immunotherapy In Metastatic Estrogen Receptor-Positive Breast Cancer, Jonathan Goldberg, Na Qiao, Jennifer L Guerriero, Brett Gross, Yagiz Meneksedag, Anne V Philips, Funda Meric-Bernstam, Jason Roszik, Ken Chen, Rinath Jeselsohn, Sara M Tolaney, George E Peoples, Gheath Alatrash, Elizabeth A Mittendorf

Faculty, Staff and Student Publications

UNLABELLED: Estrogen receptor-positive (ER+) breast cancer is not considered immunogenic and, to date, has been proven resistant to immunotherapy. Endocrine therapy remains the cornerstone of treatment for ER+ breast cancers. However, constitutively activating mutations in the estrogen receptor alpha (ESR1) gene can emerge during treatment, rendering tumors resistant to endocrine therapy. Although these mutations represent a pathway of resistance, they also represent a potential source of neoepitopes that can be targeted by immunotherapy. In this study, we investigated ESR1 mutations as novel targets for breast cancer immunotherapy. Using machine learning algorithms, we identified ESR1-derived peptides predicted to form stable complexes …

Cagi, The Critical Assessment Of Genome Interpretation, Establishes Progress And Prospects For Computational Genetic Variant Interpretation Methods, Critical Assessment Of Genome Interpretation Consortium

Faculty, Staff and Student Publications

BACKGROUND: The Critical Assessment of Genome Interpretation (CAGI) aims to advance the state-of-the-art for computational prediction of genetic variant impact, particularly where relevant to disease. The five complete editions of the CAGI community experiment comprised 50 challenges, in which participants made blind predictions of phenotypes from genetic data, and these were evaluated by independent assessors.

RESULTS: Performance was particularly strong for clinical pathogenic variants, including some difficult-to-diagnose cases, and extends to interpretation of cancer-related variants. Missense variant interpretation methods were able to estimate biochemical effects with increasing accuracy. Assessment of methods for regulatory variants and complex trait disease risk was …

Comparative Genomics Incorporating Translocation Renal Cell Carcinoma Mouse Model Reveals Molecular Mechanisms Of Tumorigenesis, Gopinath Prakasam, Akhilesh Mishra, Alana Christie, Jeffrey Miyata, Deyssy Carrillo, Hui Ye, Yunguan Wang, Oscar Reig Torras, Ramesh Butti, Hua Zhong, Jeffrey Gagan, Kevin B Jones, Thomas J Carroll, Zora Modrusan, Steffen Durinck, Mai-Carmen Requena-Komuro, Noelle S Williams, Ivan Pedrosa, Tao Wang, Dinesh Rakheja, Payal Kapur, James Brugarolas

Faculty, Staff and Student Publications

Translocation renal cell carcinoma (tRCC) most commonly involves an ASPSCR1-TFE3 fusion, but molecular mechanisms remain elusive and animal models are lacking. Here, we show that human ASPSCR1-TFE3 driven by Pax8-Cre (a credentialed clear cell RCC driver) disrupted nephrogenesis and glomerular development, causing neonatal death, while the clear cell RCC failed driver, Sglt2-Cre, induced aggressive tRCC (as well as alveolar soft part sarcoma) with complete penetrance and short latency. However, in both contexts, ASPSCR1-TFE3 led to characteristic morphological cellular changes, loss of epithelial markers, and an epithelial-mesenchymal transition. Electron microscopy of tRCC tumors showed lysosome expansion, and functional studies revealed simultaneous …

Somatostatin Receptor Subtype-2 Targeting System For Specific Delivery Of Temozolomide, Solmaz Aghaamiri, Sukhen C Ghosh, Servando Hernandez Vargas, Daniel M Halperin, Ali Azhdarinia

Faculty, Staff and Student Publications

Temozolomide (TMZ) is a DNA alkylating agent that produces objective responses in patients with neuroendocrine tumors (NETs) when the DNA repair enzyme O6-methylguanine-DNA methyltransferase (MGMT) is inactivated. At high doses, TMZ therapy exhausts MGMT activity but also produces dose-limiting toxicities. To reduce off-target effects, we converted the clinically approved radiotracer 68Ga-DOTA-TOC into a peptide-drug conjugate (PDC) for targeted delivery of TMZ to somatostatin receptor subtype-2 (SSTR2)-positive tumor cells. We used an integrated radiolabeling strategy for direct quantitative assessment of receptor binding, pharmacokinetics, and tissue biodistribution. In vitro studies revealed selective binding to SSTR2-positive cells with high affinity (5.98 ± 0.96 …

Isolation And Culture Of Epichloё Sp. For Re-Infection Of Endophyte-Free Southeastern Wildrye (Elymus Glabriflorus), B. J. Haile, J. I. Morrison

IGC Proceedings (1977-2023)

Mutualistic relationships between endophytic fungi and grasses have shown to improve the hardiness of the host. This relationship is common in grasses, including native cool-season grasses that are important in both forage and grassland ecosystems. Elymus genus members, such as Canada wildrye (CAWR), commonly host the endophytic fungi, Epichloё, while southeastern wildrye (SEWR) may not. In this study, seed of ten Elymus accessions and seed and leaves from local SEWR were assessed for endophyte infection. Infection status was confirmed via seed squash and leaf peel techniques and assessed using microscopy. Seed of one SEWR and nine CAWR accessions were assessed …

Virtual Fencing Predictable For Cattle? A Simple Method To Test Whether And How Fast Cattle Can Learn The Association Between Acoustic Signal And Electric Pulse, D. Hamidi, M. Komainda, N. Grinnell, L. Wilms, F. Reisch, J. Horn, M. Hamidi, J. Isselstein

IGC Proceedings (1977-2023)

Virtual fencing (VF) offers promising future prospects for improved grazing management as it has the potential to simplify fencing. VF lines are easily drawn and shifted via GPS coordinates. A VF collar emits an acoustic signal when the animal approaches the VF line. The signal stops immediately when the animal turns around. If the animal continues to move towards the VF line, a short electric pulse is emitted. A teaching and an operating mode are provided by the VF collars. The animals automatically change mode when they respond correctly to 20 consecutive acoustic signals without receiving an electric pulse. A …

More Than A Fencing System? Testing The Validity Of Virtual Fencing Collars For Animal Monitoring On Pasture, D. Hamidi, M. Komainda, N. Grinnell, F. Reisch, J. Horn, M. Hamidi, I. Traulsen, J. Isselstein

IGC Proceedings (1977-2023)

High labour requirements for fencing and animal monitoring appear to be general obstacles for the wider use of pastureland for grazing livestock. Virtual fencing (VF) enables a less laborious pasture management. Fence lines can be easily drawn and moved using GPS data. The advantages of VF for reducing the labour inherent to controlled grazing management are obvious. Potential additional animal monitoring opportunities arising from such a VF system that uses real-time GPS data have not yet been studied. Lying is seen as an indicator for assessing comfort or restlessness of cattle. Therefore, we focus on lying behavior in this evaluation. …

Forage And Grazinglands Extension: Training The Next Generation Of Specialists, D. W. Hancock

IGC Proceedings (1977-2023)

This invited talk provides a perspective on what is required to excel in the role as an Extension Specialist. In the USA, most such Extension Specialists are tenure-track faculty, and have state-wide or even multi-state responsibilities. Advice is given on how to balance the high expectations of such a faculty appointment while providing appropriate recommendations to farmers/ranchers and service providers in the forage and grazinglands industry. This talk will offer one former Extension Specialist’s perspective on the skills, experience, and persona required to begin a successful career as a Forage and Grazinglands Extension Specialist. Additional exposition will be given on …

An Assessment Of Young Cattle Behaviour And Welfare In A Virtual Fencing System, C. Holohan, A. Gordon, R. Palme, S. Buijs, F. Lively

IGC Proceedings (1977-2023)

Virtual fencing is a novel technology which uses a combination of audio and electrical stimuli to contain grazing livestock within a GPS boundary. There are however some concerns around the use of such a technology and its potential effects on the behaviour and welfare of animals. To investigate this, 64 dairy-origin calves were assigned to one of two treatments in a randomised complete block design, with 4 groups of 8 calves per treatment. The two treatments were virtual fencing (VF) and electric fencing (EF). The experimental period lasted 31 days in total, consisting of a 10 day training period and …

Herding Livestock – The Phoenix Rises From The Ashes? Digital Herding As A Future Tool For Grazing Livestock, J. Horn, R. Reisch, M. Komainda, P. Riedel, J. Isselstein

IGC Proceedings (1977-2023)

Today, sustainable management of grazing livestock requires high efforts in management and fencing. Nowadays, several developments in digital technologies for herding grazing animals are arising. We conducted a systematic review on current developments in digital technologies for managing grazing animals within the landscape. We mainly focused on cattle (Bos taurus) and sheep (Ovis aries). We highlight the most promising developments of virtual fencing used in recent research to evaluate effectiveness, animal behaviour and welfare. Moreover, we highlight current research in digital herding by drones and robots. We discuss the potential and current limitations of digital tools …

Development Of Megathyrsus Maximus Genotypes For Intensification Of Cattle Rearing In Brazil, Liana Jank, M. F. Santos, Sanzio Carvalho Barrios, Cacilda Borges Do Valle, Rosangela Maria Simeão

IGC Proceedings (1977-2023)

Megathyrsus maximus is a forage species used over 30 million hectares in Brazil and in the surrounding countries for intensification of the cattle production systems. Due to the large area and distinct biomes in which it is used, there is a need to continuously develop more productive and adapted cultivars to each biome and use. Three apomictic accessions were crossed with five sexual plants at Embrapa Beef Cattle in Campo Grande, MS, Brazil in 2014. More than 1100 hybrids were planted in a spaced-plant field and evaluated visually for regrowth after cuts and for disease and pest damage. In 2016, …

Scaled Datasets Through Leveraging Technology Better Represent Plant Communities Classified Within National Land Cover Database (Nlcd), D. M. Johnston, D. B. Murray, Y. Y. Tsang

IGC Proceedings (1977-2023)

Technological advances in remote sensing have allowed for access to and application of large- scale datasets of plant communities using cover classification and chlorophyll content measurement with the use of multispectral sensors and vegetation indices. The United States’ primary remote sensing platform Landsat Thematic Mapper is a series of satellites providing 30-meter resolution imagery of the Earth’s surface. Efforts have been made to best utilize Landsat derivative National Land Cover Database (NLCD) datasets and provide spatial resolution commensurate with the needs of plant ecologists and range managers. However, usage of 30-meter spatial resolution imagery and indices does not allow detection …

Heavy Grazing Significantly Reduced The Temporal Stability Of Aboveground Biomass, X. Ju, T. T. Shen, Q. Wu, G. D. Han

IGC Proceedings (1977-2023)

The stability of a plant community is the embodiment of the dynamic balance achieved by the interaction between populations in the form of competition or mutual benefit. Temporal stability refers to the ratio of the average value to the variance of the biomass of the population or community. For grassland ecosystems, the stability of the grassland plant community is the basis for its survival and functional performance, and is the key factor affecting its structure and function. In order to study the impact of grazing on the temporal stability of aboveground biomass of desert steppe, this study used a completely …

Migration And Colonization Characteristics Of Endophytic Tracer Rhizobia To The Reproductive Organs Of Alfalfa, W. J. Kang, S. L. Shi, Y. T. Zhang, L. Y. Chen

IGC Proceedings (1977-2023)

Legume seeds are the product of long-term natural selection and the co-evolution of rhizobia and seeds. They are more competitive than the native rhizobia in constructing symbiotic nitrogen-fixing systems with host plants. However, the paths and impact factors of rhizobia invasion and migration in plants, as well as colonization in seeds, are unclear. Here, alfalfa cultivars WL343HQ and Gannong 5, and three cyan fluorescent protein-tagged rhizobia (FTR), 3436f, 12531f and gn5f were used as materials. The effects of potassium dihydrogen phosphate (KH2PO4) and fulvic acid (FA) on the migration and colonization of FTR in the reproductive organs of field plants …

Spring Grass Availability And Silage Supplementation Impact On Dry Matter Intake And Enteric Methane Emissions In Grazing Dairy Cattle, M. Kennedy, S. Walsh, T. M. Boland, M. Egan

IGC Proceedings (1977-2023)

Enteric methane (CH4) emissions were measured in forty spring calving cows offered one of two (n=20) contrasting diets (High Grass; HG, Low Grass; LG) over 10 weeks in early lactation (8th February – 18th April 2021). All cows were blocked for breed and parity and balanced on milk production, economic breeding index (EBI), bodyweight and body condition score and randomly allocated to treatments. The HG grazing treatment cows were offered their full daily nutrient requirement from grazed grass and concentrate with no silage supplementation. The LG grazing treatment cows were offered a restricted (~60%) amount of their daily nutrient requirement …

Role Of Social Media And Digital Resources In Extension, L. L. Baxter

IGC Proceedings (1977-2023)

Social media and digital resources can be powerful tools for Extension personnel as they can enable forage-livestock researchers and Extension specialists to connect with a wide audience of producers, Extension personnel, colleagues, and industry partners. Online platforms that provide a space for Extension personnel to deliver timely information and digital resources have been critical for continuing producer education in today’s environment. This presentation in the thematic session on “Understanding the role of social media and digital resources in forage education and promotion” will focus on the role social media and digital resources play in Extension. There are a host of …

Creating An Educational Experience That Fosters Diversity And Inclusion, M. M. Kohmann

IGC Proceedings (1977-2023)

Demographic, intellectual, and individual diversity have been linked to increase in innovation, problem-solving, critical thinking, and economic gains. However, there are multiple challenges associated with recruiting, retaining, and advancing underrepresented groups in Science, Technology, Engineering, and Mathematics (STEM). Studies have indicated negative implicit bias against women and minorities in terms of their perceived competence, worthiness to be hired, and payment irrespective to their qualification. Additionally, lack of mentoring and access to personal capital often hinder students’ academic advancement in STEM. However, there have been multiple intentional, recent efforts to increase and foster diversity in sciences. Some of the most effective …

Modeling The Biomass Production Of Grasslands Of Wallonia According To Their Functional Type, U. Kokah Essomandan, D. Knoden, B. Dumont, J. Bindelle

IGC Proceedings (1977-2023)

Permanent grasslands are complex ecosystems which respond with a great variability - in terms of specific richness - to soil type and management strategies. Modelling is a valuable tool to explore these relationships. Our work consisted in adapting the Moorepark St Gilles grass growth model (MoSt GG) designed to model Lolium perenne plant functional type (PFT) pastures (PFT A) to a different PFT (Dactylis glomerata, PFT B) through literature-based parametrization. The model was evaluated under Walloon (Belgium) conditions using growth trials from 2014 to 2018 in two sites with contrasting pedo-climatic conditions. Three to five cuts were performed …