Life Sciences Commons^™

One-Pot Synthesis Green Zinc Oxide Nanoparticles Immobilized On Activated Carbon Derived From Pineapple Peel For Adsorption Of Pb(Ii), Vienna Saraswaty, Evyka Setya Aji, Ardi Ardiansyah, Ayu Hanifah, Nathania Puspitasari, Shella Permatasari Santoso, Sandy Budi Hartono, Chandra Risdian, Elsy Rahimi Chaldun, Henry Setiyanto

Karbala International Journal of Modern Science

The current study introduces a one-pot technique for synthesizing an environmentally benign and cheap composite adsorbent, namely ZnO-PPAC, for the adsorption of Pb(II). The designated adsorbent was prepared by incorporating green synthesized zinc oxide (ZnO) nanoparticles on activated carbon-derived from pineapple peel. The prepared adsorbents were characterized using XRD, SEM, EDS, FTIR, and BET techniques. The XRD pattern verifies that the ZnO was successfully synthesized and immobilized onto the PPAC in a one pot synthesis system. The surface areas of ZnOPPAC and PPAC adsorbents were 13.62 m2/g and 961.96 m2/g, respectively. The FTIR evaluation of the ZnO-PPAC adsorbent revealed several …

Gut Check: Exploring The Role Of Acinetobacter In Intestinal Inflammation, Janiece Sharmell Glover

MUSC Theses and Dissertations

Inflammatory Bowel Disease (IBD) is a chronic disorder characterized by excessive intestinal inflammation. Alterations in the gut microbiota have been consistently observed in individuals with IBD, but it is unclear which bacteria participate in driving inflammation. A comprehensive survey of publicly available RNA-sequencing datasets revealed that Acinetobacter species are elevated in the gastrointestinal tract of IBD patients. We found that A. calcoaceticus was particularly elevated in Crohn’s Disease patients; a subset of IBD. It is well documented that Acinetobacter species are resistant to several antibiotics, but there is very little information on the effects of Acinetobacter in the gut and …

Sustainability And Drivers Of Populus Tremuloides Regeneration And Recruitment Near The Southwestern Edge Of Its Range, Connor D. Crouch, Nicholas P. Wilhelmi, Paul C. Rogers, Margaret M. Moore, Kristen M. Waring

Aspen Bibliography

Quaking aspen (Populus tremuloides Michx.) ecosystems are highly valued in the southwestern United States because of the ecological, economic, and aesthetic benefits they provide. Aspen has experienced extensive mortality in recent decades, and there is evidence that many areas in Arizona, United States lack adequate recruitment to replace dying overstory trees. Maintaining sustainable levels of regeneration and recruitment (i.e. juveniles) is critical for promoting aspen ecosystem resilience and adaptive capacity, but questions remain about which factors currently limit juvenile aspen and which strategies are appropriate for managing aspen in an increasingly uncertain future. To fill these critical knowledge gaps, …

E Afua Mai Mauga Manuia O Nu'u: Conservation From Mountains To Reefs In Tropical Pacific Islands, Tavailau T. Segi

Theses and Dissertations

Soil erosion is the main exacerbating threat to climate change impacts in tropical Pacific islands, particularly to steep and mountainous volcanic islands. The morphological features (steep and mountainous), short distance from ridge to reef, frequent storm events, extended raining season, and accelerating developments from growing populations pose serious threats to terrestrial and marine resources. Perhaps the most threatening part is the high dependency of their human populations on the land and ocean for food supplies, supporting national economies, and sustaining culture and human well-being. Locals, governments, and non-governmental organization projects are underway to improve protection and management of natural resources …

Inferring Function From Form: Subcortical Projection Cell Types In Rat Orbitofrontal Cortex, Suelynn Ren

Arts & Sciences Graduate Student Theses and Dissertations

Frontal cortex supports sophisticated behaviors by controlling phylogenetically older subcortical brain regions. Within the deep layers of cortex, subcortically projecting pyramidal neurons integrate local and long-range inputs along the entire depth of the cortical column. How subcortically projecting neurons might be fractionated into cell types and how their unique features constrain top-down communication is unknown. In this dissertation, I first established the output circuit architecture of subcortically projecting neurons in rat orbitofrontal cortex at a cellular resolution. I found that subcortically projecting neurons preferentially innervated a single target, demonstrating a one-neuron-one-target projection logic. Such target-defined projection neurons were molecularly distinct …

Treatment Of Warthin’S Tumors Of The Parotid Gland With Radiofrequency Ablation: A Systematic Review Of The Current Literature, Kenny Do, Eric Kawana, Sisi Tian, Jo-Lawrence Bigcas

School of Medicine Faculty Research

Objective: Warthin’s tumors of the parotid gland can be safely observed. Definitive treatment usually requires parotidectomy under general anesthesia. The decision to operate on Warthin’s tumors of the parotid gland can be complicated in patients who wish to avoid risks of surgery and general anesthesia. This systematic review explores the potential of radiofrequency ablation (RFA) as a minimally invasive alternative. Methods: The Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) model was used to collect 3 relevant studies that focused on RFA treatment for Warthin’s tumors. The cumulative averages for tumor size and cosmetic scores were then quantified for …

Influence Of Forest Management On Bumblebee-Plant Networks And Community Compositions In Eastern Kentucky Forest Openings, Kassandra Carter

Online Theses and Dissertations

Both the productivity of agricultural systems and functioning of terrestrial ecosystems are reliant on animal pollinators to facilitate plant reproduction. Yet the integrity of these pollination services is threatened by the declines of pollinators. Mounting evidence indicates landscape homogenization in agricultural, urban, and most notably in natural systems have corresponded with substantial changes in bumblebee distribution, abundance, composition, and forage resource availability. Naturally occurring forest openings, barrens, and grasslands have historically provided important habitat for native bees across the otherwise forested landscape in the eastern United States. Fire suppression and the loss of these important habitats are well-documented, but it …

Overall And Sex-Specific Associations Of Serum Lipid-Soluble Micronutrients With Metabolic Dysfunction-Associated Steatotic Liver Disease Among Adults In The United States, Weiwen Chai, Meng-Hua Tao

Department of Nutrition and Health Sciences: Faculty Publications

This study examined overall and sex-specific associations of serum lipid-soluble micronutrients including α- and γ-tocopherols, 25-hydroxy-vitamin D (25(OH)D), retinol, and six major carotenoids with metabolic dysfunction-associated steatotic lever disease (MASLD) using the 2017–2018 National Health and Nutrition Examination Survey. This analysis included 3956 adults (1991 men, 1965 women) aged ≥ 20 years. Steatotic liver disease was determined through transient elastography examination. Odds ratios (ORs) and 95% confidence intervals (95% CIs) for MASLD associated with micronutrients were estimated using logistic regressions. Higher serum α-tocopherol (highest vs. lowest quartile: OR = 1.53, 95% CI = 1.05–2.22, p = 0.03) and γ-tocopherol (highest …

The Role Of Caloric Intake On Achilles Tendon Health In Pre-Professional Ballet Dancers, Annie G. Smedley

Theses and Dissertations

Background: Achilles tendinopathy is a common and debilitating condition among female ballet dancers due to the large repetitive loading forces placed on their Achilles tendons during rehearsals and performances. Tendon health problems in females are exacerbated by a lack of understanding about how energy availability influences tendons. Ballet dancers, as aesthetic athletes, are vulnerable to low energy availability and can enter a spectrum disorder, relative energy deficiency in sport, that consists of low energy availability (with or without disordered eating), menstrual cycle dysfunction, and low bone mineral density (BMD). Aims: 1) To investigate the relationship between insufficient caloric intake and …

An Approach To Identify Gene-Environment Interactions And Reveal New Biological Insight In Complex Traits, Amy R Bentley, Alanna C Morrison, Charles N Rotimi, W James Gauderman, Dabeeru C Rao, Hugues Aschard

Faculty, Staff and Student Publications

There is a long-standing debate about the magnitude of the contribution of gene-environment interactions to phenotypic variations of complex traits owing to the low statistical power and few reported interactions to date. to address this issue, the Gene-Lifestyle Interactions Working Group within the Cohorts for Heart and Aging Research in Genetic Epidemiology Consortium has been spearheading efforts to investigate G × E in large and diverse samples through meta-analysis. Here, we present a powerful new approach to screen for interactions across the genome, an approach that shares substantial similarity to the Mendelian randomization framework. We identify and confirm 5 loci …

Predictive And Prognostic Biomarkers And Tumor Antigens For Targeted Therapy In Urothelial Carcinoma, Aditya Eturi, Amman Bhasin, Kevin Zarrabi, William Tester

Department of Medical Oncology Faculty Papers

Urothelial carcinoma (UC) is the fourth most prevalent cancer amongst males worldwide. While patients with non-muscle-invasive disease have a favorable prognosis, 25% of UC patients present with locally advanced disease which is associated with a 10-15% 5-year survival rate and poor overall prognosis. Muscle-invasive bladder cancer (MIBC) is associated with about 50% 5 year survival when treated by radical cystectomy or trimodality therapy; stage IV disease is associated with 10-15% 5 year survival. Current therapeutic modalities for MIBC include neoadjuvant chemotherapy, surgery and/or chemoradiation, although patients with relapsed or refractory disease have a poor prognosis. However, the rapid success of …

Benthic Community Response To The Impacts Of Proposed Sediment Diversions In Louisiana Estuaries, Jillian C. Tupitza

LSU Doctoral Dissertations

Sediment diversions designed to mitigate land loss in coastal Louisiana will likely cause ecosystem-wide shifts. The first diversion, planned for Barataria Bay, will divert fresh water and sediment from the Mississippi River into the surrounding wetlands to help them keep pace with high rates of relative sea-level rise (RSLR). Estuarine benthic communities were used in this study as tools to characterize ecological patterns in the context of anticipated salinity changes. I reviewed relevant literature, conducted a two-year survey of Barataria Bay benthos, executed a reciprocal transplant experiment to describe changes that might occur in both community composition and trophic dynamics, …

Exploring Pedagogical Approaches: A Comparative Analysis Of Information Delivery Methods In Fish Dissection Instruction, Kiara Smidt

Honors Projects

The Covid-19 pandemic prompted a global shift to remote work and education, challenging traditional teaching methods. This research explores the effectiveness of audiovisual versus visual-only guides in teaching perch dissection anatomy, safety, and procedure. The study involves a cross-sectional experiment with students from an Introduction to Biology course at Bowling Green State University. Participants were divided into groups using either a video or a written guide, and their knowledge was assessed before and after the dissection. Results calculated through a Student’s t-test indicate no significant difference in overall effectiveness between the two methods, apart from labeling an anatomy diagram and …

Cultivating Excellence: A Literature Review On Harnessing The Power Of The Gut Microbiome For Athletic Performance, Maya Katharine Dean

Honors Projects

The interplay between our gut microbiome and health is immense. This literature review analyzes the current research assessing the interplay between gut microbiome and athletic performance. Knowing how to improve gut microbial diversity via nutrition and supplementation can take athletic performance to the next level; namely improvements in immune, mental, and physical health.

The Effect Of Nutrition Education On Perceived Fatigue Of Collegiate Endurance Athletes, Quinn Murphy

Honors Projects

Background: Based on previous research, athletes have low knowledge when it comes to nutrition. This puts them at a greater risk for poor dietary choices that could ultimately impact their ability to perform optimally, increase fatigue, and increase the risk of injuries. The purpose of this study was to determine if after taking a nutrition education class, the athletes would better fuel their bodies and improve their perceived fatigue levels.

Methods: Initially, five BGSU club runners were recruited to participate. They were asked to track five sequential days of food and drink intake and the level of daily fatigue (consisting …

Evolution Of The Human Eye: As Compared To Other Vertebrates, Madison Queener

Honors Projects

There are three different types of eyes, the simple eye, the compound eye, and the camera eye (Cambridge Dictionary) (Myer-Rochow, 2014) (UCL, 2020). The retina of the eye has evolved and adapted to fit the lifestyles of the respective organisms. Because of this part of the eye, organisms are able to see different colors and use light to define the world using photoreceptors. Photoreceptors are rod cells, which are light sensitive and process light, and cone cells, which perceive the different color wavelengths, that pass visual information to the brain (Kazilek, 2010). About 5% of the photoreceptors in the retinas …

The Decline In Monarch Butterfly, Danaus Plexippus, Populations: An Example Of The Global Threat To Biodiversity, Olivia Sidoti

Honors Projects

Biodiversity encompasses the variety of all life on Earth and how these aspects of nature interact with each other. To have stable and abundant biodiversity, vast amounts of species and organisms are required within an ecosystem. As a result of the increase in negative impacts of human activities and behaviors on the health of nature, biodiversity has been decreasing. An example of the decrease in biodiversity is depicted by the recent decline of the monarch butterfly species. The monarch butterfly is an iconic North American insect that is experiencing a decline in its population due to threats such as deforestation, …

Edna Metabarcoding And The Construction Of Primers For The Identification Of Gulf Of Mexico Elasmobranchs And Selachii, Isabella Lima

Honors Projects

eDNA, or environmental DNA, is found free-existing in any environment. It can be extracted from its environment and used to identify organisms that were recently in the area. In recent years, eDNA metabarcoding has been used to help examine communities for overall species presence and biodiversity. This capstone sought to create a mock proposal for an eDNA survey of Gulf of Mexico elasmobranch and Selachii species due to their overall decline in numbers. Additionally, general and discriminatory PCR primers were created in-silico and assessed for initial quality. This capstone documents the feasibility of an eDNA study, constructing the necessary primers, …

Association Between Antenatal Vaginal Bleeding And Adverse Perinatal Outcomes In Placenta Accreta Spectrum, J Connor Mulhall, Kayla E Ireland, John J Byrne, Patrick S Ramsey, Georgia A Mccann

Faculty, Staff and Student Publications

Background and Objectives: Placenta accreta spectrum (PAS) disorders are placental conditions associated with significant maternal morbidity and mortality. While antenatal vaginal bleeding in the setting of PAS is common, the implications of this on overall outcomes remain unknown. Our primary objective was to identify the implications of antenatal vaginal bleeding in the setting of suspected PAS on both maternal and fetal outcomes. Materials and Methods: We performed a case-control study of patients referred to our PAS center of excellence delivered by cesarean hysterectomy from 2012 to 2022. Subsequently, antenatal vaginal bleeding episodes were quantified, and components of maternal …

Exploration Of Enterococcus Faecalis Bacteriophages Diversity: Comprehensive Phylogenetic Analysis And Discovery Isolation Of Enterococcus Faecalis Phage From A Soil Sample In South Florida, Hailey N. Jauernick

All HCAS Student Capstones, Theses, and Dissertations

Enterococcus faecalis is a commensal bacterium found in the gastrointestinal tract, oral cavity, and vagina of humans, which can transition to pathogenic states, leading to diverse infections. Many E. faecalis infections are becoming progressively challenging to treat, stemming from the lack of effective antibiotic therapies against its virulence factors, including biofilm formation and antibiotic resistance. Phage therapy emerges as a promising alternative, targeting bacteria with minimal impact on host flora. However, despite phages' ubiquitous presence and potential applications in medicine and biotechnology, their diversity and ecological niches are vastly understudied. Here, we investigated the diversity of sequenced E. faecalis phages …

The Influence Of Strength And Power Training On The Performance Of U.S. Marines, Kristopher J. Klein

Master of Science in Kinesiology

U.S. Marine Infantry conducts a job that requires strength, speed, and endurance; however military fitness training relies mainly on the endurance aspect. With numerous studies showing the correlation between an endurance mainstay within military organizations, and musculoskeletal and joint overuse injuries, this study seeks to understand the performance value in training the anaerobic energy systems. Specifically, we aim to determine whether strength and power training may be more beneficial to the tactical athlete than traditional training. Over a period of 12 weeks, 150 members of the U.S. Marine Infantry will participate in training five days a week with three days …

The Anatomical Embodiment Of Morning Routines In The Reduction Of Anxiety: An Intervention, Natalie Wright

Expressive Therapies Capstone Theses

The topic under investigation is whether physically embodying a morning routine that was designed through the lens of Laban Bartenieff Movement Analysis (LBMA) will reduce daily symptoms of individuals diagnosed with Generalized Anxiety Disorder.

Since morning routines play a significant part in one’s preparation for the day, I created an individualized LBMA morning routine for a specific client to embody. In addition to the routine, the client documented the process of their anxiety levels on a weekly basis. This client was a white, female, 19-year-old, lesbian college student who was previously diagnosed with Generalized Anxiety Disorder (GAD). The movement analysis …

Harmonizing Soybean-Associated Beneficial Bacteria For Sustainable Cultivation, Jonas J. Padilla

LSU Master's Theses

Long-term reliance on agrochemicals, such as fertilizers and pesticides, to improve crop productivity can severely impact soil physicochemical properties and health. This eventually leads us to completely depend on these chemicals to sustain the needs of our crops. To resuscitate the deteriorating state of our arable lands, we need to shift from traditional farming to more sustainable crop management. One of the alternatives that we can use instead of these chemicals is the utilization of microorganisms that can perform a variety of beneficial functions for the growth and development of crops. Recently, seed-treating materials made up of bacterial consortia were …

A Comparison Of The Prevalence Of Salmonella, Specifically S. Enteritidis, In Captive Born Versus Wild Caught Reptiles, Jessica Thomas, Carrie M. Doyle

Undergraduate Theses

Salmonella contamination and transmission is a concern in public health in the United States and around the world. It is usually transmitted to humans through contaminated poultry products, but it has also been known to be transmitted through contact with reptiles. Because reptiles are becoming more popular as pets and are known carriers for Salmonella, studies have been done to test how prevalent Salmonella is in reptiles. This study focused on testing the feces of twenty-two reptiles for Salmonella, in general, and a specific serotype of Salmonella, S. Enteritidis. This study also compared the positivity rate of reptiles born …

Evaluating Consistency Across Multiple Neospectra (Compact Fourier Transform Near-Infrared) Spectrometers For Estimating Common Soil Properties, Sadia M. Mitu, Colleen Smith, Jonathan Sanderman, Richard R. Ferguson, Keith Shepherd, Yufeng Ge

Department of Agronomy and Horticulture: Faculty Publications

Rapid and cost-effective techniques for soil analysis are essential to guide sustainable land management and production agriculture. This study aimed at evaluating the performance and consistency of portable handheld Fourier-transform near-infrared spectrometers and the NeoSpectra scanners in estimating 12 common soil physical and chemical properties including pH; organic carbon (OC); inorganic carbon (IC); total nitrogen (TN); cation exchange capacity (CEC); clay, silt, and sand fractions; and exchangeable potassium (K), phosphorus (P), calcium (Ca), and magnesium (Mg). A diverse set of samples (n = 600) were retrieved from a national-scale soil archive of the Kellogg Soil Survey Laboratory of USDA-NRCS …

Depletion Of Protein Phosphatase 1 Results In Persistent Activation Of The Integrated Stress Response And Age-Associated Exacerbation Of Pulmonary Veno-Occlusive Disease, Amit Prabhakar, Meetu Wadhwa, Prajakta Ghatpande, Rahul Kumar, Brian B. Graham, Giorgio Lagna, Akiko Hata

Biology

Pulmonary veno-occlusive disease (PVOD) is a form of pulmonary hypertension that affects individuals across the age spectrum. PVOD is characterized by the obstruction of small pulmonary vessels, causing increased pulmonary artery (PA) pressure and leading to right ventricular heart (RV) failure. Previous research showed that the administration of Mitomycin-C (MMC) in rats mediates PVOD through the activation of the eukaryotic initiation factor 2 (eIF2) kinase PKR and the integrated stress response (ISR), resulting in the impairment of vascular endothelial junctional structure and barrier function. In this study, we reveal that older rats experience more severe pulmonary vascular remodeling and RV …

Wild Seahorse Conservation And Industrial Growth Strategies: A Dual-Perspective Study, Shicheng Wang, Bo Li, Lanliang Yu

Bulletin of Chinese Academy of Sciences (Chinese Version)

The United Nations Decade of Ocean Science for Sustainable Development (2021–2030) prioritizes healthy oceans and sustainable development. Protecting marine biodiversity, promoting sustainable fisheries, and achieving global sustainability goals are critical concerns for the international community. This study addresses critical challenges in seahorse conservation and industrial development, including wild resource depletion, limitations in aquaculture technology, low-value industrial chains, and inadequate regulatory coordination. Through systematic analysis of current conservation status and industrial trends, establishing demonstration bases is proposed for seahorse resource protection and high-value utilization ("Hippocampus Hub"), guided by natural habitat integrity, scientific research capacity, industrial chain integration, and policy support. By …

Faa Medical Reform, Dana Rose

Honors Projects

This project investigates the challenges and implications of obtaining medical certification for pilots with a history of childhood misdiagnoses, focusing on Attention-Deficit/Hyperactivity Disorder (ADHD). Misdiagnoses in childhood can lead to significant obstacles in the aviation industry's medical certification process, posing unique hurdles for aspiring pilots. The study examines the current FAA guidelines and procedures for medical certification, particularly the evaluation requirements for individuals with ADHD. It also explores common challenges faced by pilots, the effects of childhood misdiagnoses on their eligibility, and the impact of the FAA's ADHD evaluation process.

Through interviews with Aviation Medical Examiners (AMEs), this project identifies …

Th2 Cells Are Associated With Tumor Recurrence Following Radiation, Mohamed K Abdelhakiem, Riyue Bao, Phillip M Pifer, David Molkentine, Jessica Molkentine, Beth Beadle, Jason J Luke, Robert L Ferris, Curtis R Pickering, Jing H Wang, Ravi B Patel, Heath D Skinner

Faculty, Staff and Student Publications

The curative treatment of multiple solid tumors, including head and neck squamous cell carcinoma (HNSCC), utilizes radiation. The outcomes for HPV/p16-negative HNSCC are significantly worse than HPV/p16-positive tumors, with increased radiation resistance leading to worse locoregional recurrence (LRR) and ultimately death. This study analyzed the relationship between immune function and outcomes following radiation in HPV/p16-negative tumors to identify mechanisms of radiation resistance and prognostic immune biomarkers. A discovery cohort of 94 patients with HNSCC treated uniformly with surgery and adjuvant radiation and a validation cohort of 97 similarly treated patients were utilized. Tumor immune infiltrates were derived from RNAseq gene …

Exposure To The Trier Social Stress Test Enhances Central Detail Memory, Reduces False Memory, And Results In Intrusive Memories That Last For Days, Mercedes L. Stanek, Kayla Boaz, Taylor D. Niese, Kristen E. Long, Matthew S. Risner, John G. Blasco, Koen N. Suzelis, Kelsey M. Siereveld, Boyd R. Rorabaugh, Phillip R. Zoladz

ONU Student Research Colloquium

Recent work has used a modified version of the well-known laboratory stressor, the Trier Social Stress Test (TSST), to study participant memory for a stressful experience. The paradigm is useful because, unlike most studies examining stress effects on memory, it allows investigators to measure what participants remember about the stressor, not unrelated information. It also presents an opportunity to model other stress-related symptoms, such as intrusive memories, but these have yet to be assessed with this paradigm. Intrusive memories have been notoriously difficult to measure in laboratory settings; most of this research involves participants watching arousing videos and subsequently reporting …