Life Sciences Commons^™

Determination Of 7 Synthetic Colorants In Freshly Prepared Beverages By High Performance Liquid Chromatography Dual Wavelength Method With Automatic Solid Phase Extraction, Yao Yuyang, Xu Man, Pan Chunyan, Miao Xiong, Xue Qiang

Food and Machinery

Objective: A method for the determination of 7 synthetic colorants in freshly prepared beverages by high performance liquid chromatography dual （HPLC） wavelength method with automatic solid phase extraction was established. Methods: Extraction conditions: The sample was extracted 2 times with pure water, and then the supernatant combined after centrifugation. The pH of the supernatant was adjusted to 3~4. After activation by an automatic solid-phase extraction device, loading, rinsing, and elution of the sample were carried out on the Poly-sery synthetic colorant solid-phase extraction column. The eluent was concentrated to 200 μL by nitrogen blowing, and then fixed to 1.0 mL …

Determination The Residues Of Matrine And Oxymatrine In Liupao Tea By Uplc-Ms/Ms With Isotope Internal Standard Method, Shi Jine, Zhang Hongyu, Xin Ruozhu, Ding Mei, Xi Guangsheng

Food and Machinery

Objective: Taking Guangxi Liupao tea as an example, an UPLC-MS/MS analysis method with residual amounts of matrine and Oxymatrine was established. Methods: The prepared samples were subjected to optimized acetonitrile aqueous solution （80% acetonitrile+0.2% ammonia water） ultrasonic extraction, mobile phase: formic acid water and formic acid methanol （both 0.1%）, gradient elution, and internal standard method quantification. Results: It was linear in the range of 0.1～80.0 ng/mL, r was greater than 0.999 6, the detection limits of matrine and oxymatrine were 1.0 μg/kg, and the quantitative limits were 3.0 μg/kg, which met the regulatory determination requirements for the residue of matrine …

Analysis Of Uncertainty In Determination Of Folic Acid In Milk Powder By Microplate Method, Liu Ziyin, Zhuo Yiming, Liu Lalan, Peng Na, Wu Houlu

Food and Machinery

Objective: To analyze and evaluate the uncertainty of the determination of folic acid content in milk powder by microplate method. Methods: The content of folic acid in infant formula milk powder was determined by microplate method according to GB 5009.211—2022 "National Standard for Food Safety-Determination of Folic Acid in Foods". Various experimental factors affecting the test results were analyzed and calculated according to the uncertainty evaluation methods and guidelines such as JJF 1059.1—2012, CNAS-CL01-G003:2011 and CNAS-GL05:2011, and the synthetic standard uncertainty and expanded uncertainty were obtained. Results: The expanded uncertainty U₉₅=22.08 μg/100 g（k=2）, and the folate …

Study On The Performance Of Heat Pump Assisted Random Filler Surface Evaporation Device For Concentrated Thermosensitive Feed Liquid, Ma Yinglu, Chen Dong, Hu Shengwei, Wang Shuo, Zhang Huijuan, Xie Jihong

Food and Machinery

Objective： A surface evaporation concentration device were designed to concentrate thermosensitive feed liquid at low temperature under normal pressure with low energy consumption. Methods： Based on an introduction to the working principle of the device, the basic equations of the device were given. The changes of volumetric evaporation flux and energy saving ratio with flow rate of the feed liquid, feed liquid temperature, sweeping gas flow rate, sweeping gas temperature were calculated and analyzed. Designed an experimental platform and obtained a set of optimal performance parameters through experiments. Results： The results showed that when the flow rate of the feed …

Research On The Tooth Roll Power Of Roller Mill Based On Discrete Element, Zhao Qifeng, Wu Wenbin, Wang Xuefeng, Jia Huapo

Food and Machinery

Objective: To optimize the operating parameters of wheat milling in roller mill I skin mill to reduce the energy consumption of milling. Methods: Using EDEM discrete element simulation software, Box-Behnken test was designed for key milling factors such as roll pitch, feeding amount, rotational speed and rotational speed ratio to investigate the relationship between operating parameters and milling power. Results: The significance rankings of the effects on the toothed roll crushing power were, in order, speed ratio, roll pitch, feed volume, speed ratio quadratic term, roll pitch quadratic term, interaction of roll pitch and speed ratio, interaction of feed volume …

Robot's Automatic Strategy For Fermented Grains Scooping Based On Machine Vision, Tian Huairui, Tian Jianyan, Wang Sugang, Wang Xiaobo

Food and Machinery

Objective: To solve the problems of low level of automation and high labor intensity of workers in the fermented grains discharging process of solid-state fermentation in the ground-pot. Methods: An automatic fermented grains scooping strategy for the compound robot for fermented grains scooping was proposed. Based on the pre-processing of the ground-pot image and the reconstruction of the point cloud inside the inner edge, the point cloud segmentation algorithm was used to extract the point cloud of fermented grains. The scooping plane was constructed. The safe working distance for scooping was solved. Preset scooping points were set. Afterwards, the scooping …

Effect Of Chlorine Dioxide Fumigation Treatment On Quality Of Broccoli, Li Yuqing, Lu Lixin, Wang Qing

Food and Machinery

Objective： This study aimed to solve the problems of yellowing, discoloration and quality preservation of broccoli after harvesting. Methods： Broccoli was fumigated with chlorine dioxide gas at different mass concentrations (0,0.2, 0.5, 1.0, 1.5, 2.0 mg/L) at 23 ℃, and the sensory, color, yelination grade, physiological and biochemical indexes and related browning enzyme activities of broccoli were determined after 0,2,4,6,8 days of storage. Results： Compared with the control group, different mass concentrations of ClO₂ could inhibit the mass loss rate, yellowing browning and spoilage of flower buds of broccoli. ClO₂ at 0.5 and 1.0 mg/L had a more …

Study On The Performance Of Porcine Liver Protein Composite Membrane And Its Application In Meat Preservation, Yang Le, Liu Lili, Wang Haoyang, Chen Hui, Ding Yue

Food and Machinery

Objective: A new type of natural food preservative with pork liver as the main raw material was developed. Methods: Water-soluble porcine liver protein (WSLP), salt-soluble porcine liver protein (SSLP) and chitosan (CS) were combined to create edible composite membranes. The edible membrane components were then examined from a variety of perspectives using mechanical properties, Fourier transform infrared spectroscopy (FT-IR) and microstructure analysis. Utilized to preserve pork, and by contrasting the changes in the physical and chemical indices during the chilling process, the freshness retention capabilities of the composite film were investigated. Results: With the addition of WSLP and SSLP, the …

A Preliminary Study On The Effect Mechanism Of A Rice Immune Peptide In Mice, Wen Shiyu, Zhang Zhimeng, Wu Hao, Xie Yufei, Huang Qingming, Liao Juan, Wen Li

Food and Machinery

Objective: This study aimed to demonstrate the potential immunomodulatory activity in mice of rice immunopeptide GBP1 （NSVFRALPVDVVANAYR） screened previously. Methods: Animal experiments were conducted to investigate the immunomodulatory mechanism of GBP1 peptide. RNA-Seq was used to sequence the transcriptome of mouse spleen tissues before and after GBP1 peptide treatment, and the molecular mechanism of GBP1 peptide immunomodulatory effect was explained. Results: In the GBP1 peptide treatment group, the number of spleen cells tended to be normal, the liver organ coefficient decreased significantly, and the serum IgG content was stable.GBP1 peptide can affect the expression of cytokines, mediate signal transduction pathways, …

The Antitumor Activity Of Brassica Rapa L. Acid Polysaccharides In Vivo, Kadeer Gulimila, Maitiruze Amannisa, Li Mingzhu, Kang Jinsen, Taoerdahong Hailiqian

Food and Machinery

Objective: To explore the effects of Brassica rapa L. acid polysaccharide 1 (BRAP-1) on H226 cell tumor-bearing mice. Methods: 60 mice were divided into the model group (0.1 mL/10 g), positive cisplatin [3 mg/(kg·2 d)], BRAP-1 low dose [50 mg/(kg·d)], BRAP-1 medium dose [100 mg/(kg·d)] and BRAP-1 high dose [200 mg/(kg·d)]. During the experiment, the tumor growth of mice was observed, and the tumor inhibition rate and organ index were calculated; The levels of interleukin-18 (IL-18), interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) in serum were detected by enzyme-linked immunosorbent assay. The gene expressions …

Analysis Of Flavor Characteristics Of Herbal Cultivated Tremella Fuciformis Based On Component Difference Analysis And Intelligent Sensory, Chen Wanchao, Wu Di, Xiao Minsheng, Yao Shuxian, Yang Yan, Liu Yanfang

Food and Machinery

Objective: Clarify the flavor characteristics of Tremella fuciformis cultivated on herbaceous substrate. Methods: In the study T. fuciformis cultivated on herbaceous substrate was used as the research object, while samples of yellow tremella and white tremella cultivated on conventional substrate were used as controls. The differences in flavor composition between them were analyzed using high-performance liquid chromatography and Headspace Solid Phase Microextraction Ggas Chromatography Mass Spectrometry (HS-SPME-GC-MS). And combined with intelligent sensory technology, its flavor characteristics were analyzed. Results: According to multi-dimensional evaluations such as fresh and sweet amino acid and flavor nucleotides content, taste activity value and equal freshness …

Effects Of Grape Seed Proanthocyanidins On Hepg2 Cell Genes And Related Functions Based On Transcriptome Sequencing, Zheng Wancai, Dang Bin, Zhang Ji, Luo Qiaoyu, Feng Zuoshan

Food and Machinery

Objective： Based on transcriptome sequencing analysis, the effect of grape seed procyanidins on HepG2 cell genes and related functions was identified. The key genes and metabolic pathways involved in the treatment of HepG2 cells with procyanidins were confirmed. Methods: Through transcriptome sequencing, screening differential genes, gene functional annotation and enrichment analysis of the KEGG pathway, transcriptome studies were conducted on grape proanthocyanidin-treated cells. Results: After treating HepG2 cells with grape anthocyanins, the main biological processes enriched are cellular processes, metabolic processes, biological regulatory processes, immune system processes, reproductive regulation, and growth regulation. Research has found that 12 key differentially expressed …

Optimization Of Anthocyanin Copigmentation Formula Of Purple Corn And Its Effect On The Quality Of Purple Corn, Pang Wenqian, Yu Rui, Li Dajing, Liu Chunju, Bai Bing

Food and Machinery

Objective: This study aimed to explore the copigmentation formula of purple corn anthocyanins. Methods: In this study, fructose, tannic acid, and pectin were used as co-pigmentation. The response surface method was used to optimize the co-pigmentation formula of purple corn anthocyanins, and the untreated purple corn was used as a control test. The anthocyanin components, color, texture, taste and antioxidant activity (DPPH free radical scavenging ability, ABTS free radical scavenging ability, and FRAP iron ion reducing ability) of purple corn after different treatments were determined. Results: 19% fructose, 0.07% tannic acid, and 1.9% pectin had the best copigmentation effect. Compared …

Study On Preparation, Characterization And Stability Of Β-Cyclodextrin Inclusion Compound Of Foeniculum Vulgare Volatile Oil, Zhang Yumeng, Xu Fang, Yao Yuhan, Li Chenyang, Zhao Jun

Food and Machinery

Objective: To Prepared of β-Cyclodextrin inclusion complex with Foeniculum vulgare（fennel） volatile ooil and study on its sstructural characterization and stability. Methods: Based on the inclusion rate, the inclusion yield and the content of trans-anethole in the inclusion compound, the comprehensive evaluation index was established, and the effects of the ratio of fennel volatile oil to β-cyclodextrin, inclusion temperature and inclusion time on it were explored. The preparation process was optimized by orthogonal test. It was characterized by scanning electron microscopy, thin layer chromatography, Fourier infrared spectroscopy and differential scanning calorimetry. Finally, the stability of the inclusion compound was …

Effects Of Drying Methods On The Quality And Volatile Flavor Compounds Of Oil Tea Powder, Li Guanli, Wu Qiuyue, Chen Xixia, Wu Shujie, Duan Qiuxia, Li Xiaochun, Luo Yanghe

Food and Machinery

Objective: This study aimed to explore suitable drying methods for oil tea powder, making it easier for oil tea consumption and storage. Methods: Spray drying, vacuum freeze drying and vacuum drying were used to dry oil tea soup, and the quality (moisture content, sensory evaluation and color) and volatile substances of dried oil tea powder were evaluated. Results: The sensory score of vacuum freeze-drying oil tea powder was the highest (93), the water content was the lowest (3.78%), and the color was better（ΔE value 49.74). 104 volatile substances were identified from three drying methods of oil tea powder using …

Research Progress On The Mechanism Of Food-Derived Antihypertensive Peptides, Wang Baobei, Zhang Hui, Liu Yusong, Chen Hongbin, Guo Fengxian, Zheng Zongping

Food and Machinery

In this review, several mechanisms of antihypertensive peptides derived from food and its by-products were summarized. Mechanisms based on the RAAS system such as ACE/Ang Ⅱ/AT1R signaling pathways and ACE2/Ang (1-7)/MasR signaling pathways were included. Mechanisms targeting at KNOS system including PI3K/Akt/eNOS signaling pathway, PPAR-γ/caspase3/MAPK/eNOS signaling pathway and L-type Ca²⁺ channel were also discussed. In addition, the pathways of reducing blood pressure by inhibiting endothelin-converting enzyme (ECE) activity discovered in recent years were also introduced.

Ketoprak Dor: Awal Mula Keberadaannya Di Aceh Tengah, Susandro Susandro, Afrizal H

Paradigma: Jurnal Kajian Budaya

This article discusses the origin of the Ketoprak Dor art in Paya Tumpi Kampong, Kebayakan District, Central Aceh. This form of art is interesting because it demonstrates that change or development of an art does not only arise from the artist’s own creative processes, but it may also occur due to external factors. Thus, a qualitative research approach with descriptive analysis method was used to investigate this cultural phenomenon. This research proves that Ketoprak Dor originated from Deli, North Sumatra. There were two events that prompted its emergence. First, in 1950s, a Ketoprak Dor group from Deli performed in Bies …

Molecular Insights Of Paraoxonase 2 In Advanced Glycation End Product Induced Endothelial Dysfunction, Ramya R

Theses and Dissertations

Diabetes mellitus (DM) is a metabolic disorder associated with various vascular complications and affects the function of the endothelial cells. Elevated blood glucose levels accelerate the formation of advanced glycation end products (AGEs) by Amadori rearrangement and bind to their Receptor for the advanced glycation end product (RAGE) and evoke release of key pro-inflammatory cytokine proteins leading to endothelial dysfunction. Paraoxonase 2 (PON2) is an endogenous intracellular enzyme, localised in mitochondria, endoplasmic reticulum (ER), and nuclear membrane with numerous properties, like anti-inflammatory, anti-apoptotic and anti-oxidant.

Our present study aims to ascertain the effect of AGEs on PON2 activity and expression, …

The Immune Contribution To Postpartum Remodeling, Jessica Caroline Ming

Master's Theses

Following parturition, the uterus must undergo significant changes in size and structure through a process called postpartum remodeling. This process is known to have both pro- and anti- inflammatory components. However, how various immune cells contribute to remodeling and the pathways that promote inflammation are not well understood. We hypothesize that immune cells that infiltrate the uterus postpartum modulate inflammation to promote postpartum remodeling. Our preliminary data suggests that inflammatory cell death may contribute to the inflammation present in the early postpartum period, and thus further studies will analyze caspase-1, gasdermin D, and IL-1β and IL-18. To analyze the immune …

A Sublethal Concentration Of Chlorine Induces Antibiotic Resistance In Salmonella Via Production Of Reactive Oxygen Species, Mohammed Aljuwayd, Israa Abdullah Malli, Steven C. Ricke, Young Min Kwon

Poultry Science Faculty Publications and Presentations

Studies have shown that the production of reactive oxygen species (ROS) is triggered by bactericidal antibiotics, which contributes significantly to the killing of bacterial cells and increasing mutations in surviving cells. In this study, we hypothesized that exposure of Salmonella to sublethal concentrations of hypochlorite (NaOCl), a commonly used sanitizer in household and food industries increases mutation rates, leading to the development of antibiotic resistance. We found that a sublethal concentration (20 ppm) of NaOCl increased the mutation rates of S. typhimurium 14028s significantly (p < 0.05), which was prevented by the ROS scavenger thiourea, supporting that the increased mutation was due to NaOCl-triggered ROS production. We further found that the exposure of S. typhimurium 14028s to the same sublethal concentration of NaOCl increases resistance to kanamycin among the 3 antibiotics evaluated. The results of this study suggest that when NaOCl applied as a sanitizer fails to kill Salmonella due to diluted local concentrations or presence of organic materials, it can cause an adverse outcome of developing antibiotic resistance of the pathogen.

Decorin Suppresses Tumor Lymphangiogenesis: A Mechanism To Curtail Cancer Progression, Dipon K. Mondal, Christopher Xie, Gabriel J. Pascal, Simone Buraschi, Renato V. Iozzo

Kimmel Cancer Center Faculty Papers

The complex interplay between malignant cells and the cellular and molecular components of the tumor stroma is a key aspect of cancer growth and development. These tumor-host interactions are often affected by soluble bioactive molecules such as proteoglycans. Decorin, an archetypical small leucine-rich proteoglycan primarily expressed by stromal cells, affects cancer growth in its soluble form by interacting with several receptor tyrosine kinases (RTK). Overall, decorin leads to a context-dependent and protracted cessation of oncogenic RTK activity by attenuating their ability to drive a prosurvival program and to sustain a proangiogenic network. Through an unbiased transcriptomic analysis using deep RNAseq, …

Feminist Political Ecology In The Classroom, Ella J. Yeigh

Northwest Journal of Teacher Education

As the effects of climate change are being felt more frequently, discussions on how to combat such a massive issue are increasingly prevalent. Finding solutions to the climate crisis requires an understanding of how mainstream economic systems have led to the climate crisis and using these same principles to get out of the climate crisis is misguided. Economic actions have inherent value biases that have real political effects. Feminist Political Ecology (FPE) as a theoretical model presents a better understanding of how values that are inherent in economic models such as reliance on efficiency, markets, and continual economic growth have …

Learning To Teach About Climate Justice And Social Justice In Science Methods, Mindy J. Chappell

Northwest Journal of Teacher Education

In November, the Editors of NWJTE sat down for a conversation with Dr. Mindy J. Chappell, a Science Teacher Educator in the College of Education at Portland State University. Dr. Chappell’s passions include developing teachers who are prepared to disrupt normative science ideologies and provide young people with science instruction that encourages and empowers them to be leaders in their communities. She engages in arts-based educational science research through the methodology of Ethnodance (a term she coined). She places young people and their lived experiences at the heart of her work.

Overexpression Of Fatty Acid Synthase Upregulates Glutamine–Fructose-6-Phosphate Transaminase 1 And O-Linked N-Acetylglucosamine Transferase To Increase O-Glcnac Protein Glycosylation And Promote Colorectal Cancer Growth, James Drury, Mariah E. Geisen, Josiane Weber Tessmann, Piotr G. Rychahou, Courtney O. Kelson

Markey Cancer Center Faculty Publications

Fatty acid synthesis has been extensively investigated as a therapeutic target in cancers, including colorectal cancer (CRC). Fatty acid synthase (FASN), a key enzyme of de novo lipid synthesis, is significantly upregulated in CRC, and therapeutic approaches of targeting this enzyme are currently being tested in multiple clinical trials. However, the mechanisms behind the pro- oncogenic action of FASN are still not completely understood. Here, for the first time, we show that overexpression of FASN increases the expression of glutamine–fructose-6-phosphate transaminase 1 (GFPT1) and O-linked N-acetylglucosamine transferase (OGT), enzymes involved in hexosamine metabolism, and the level of O-GlcNAcylation in vitro …

Genetic, In Vitro Phenotypic, And Clinical Characterization Of Atypical Enteropathogenic E. Coli Infection And Pathogenesis, Sarah E. Kralicek

Dissertations

Enteropathogenic E. coli (EPEC) are enteric pathogens that are non-invasive, lack Shiga toxin, and use a type 3 secretion system encoded on the locus of enterocyte effacement (LEE) pathogenicity island to translocate bacterial effector proteins into host intestinal epithelial cells leading to diarrhea. Atypical EPEC (aEPEC), in contrast to typical EPEC (tEPEC), lack bundle-forming pili, likely altering adherence and pathogenicity. Detection of aEPEC with co-infecting pathogens and in some asymptomatic individuals leads to questions regarding aEPEC virulence, especially in adults. I aimed to characterize the clinical manifestations of aEPEC infection and the genetic and in vitro phenotypic factors that contribute …

Elucidating The Underlying Mechanisms Polyamines Play On Coxsackievirus B3 Infection, Mason Richard Firpo

Dissertations

Polyamines are a class of metabolites consisting of small carbon chains that contain amine groups and are positively charged at cellular pH. They play many important roles within the cell and are necessary for cell growth, membrane function, transcription, and translation. They are also required by a myriad of viruses for a productive infection. This includes the non-polio enterovirus Coxsackievirus B3 (CVB3). CVB3 is a cardiotropic virus that can readily infect the heart and cause irreversible damage which can eventually lead to dilated cardiomyopathy and the need for a heart transplant. Unfortunately, there are no approved antivirals or vaccines that …

The Role Of Extracellular Vesicles And Their Cargo In Intercellular Communication, Chongyu Zhang

Dissertations

Extracellular vesicles (EVs) are membranous vesicles secreted by most cells. They contain lipids, proteins, and nucleic acids, including microRNAs (miRNA), and function in intercellular communication by endocytosis into other cells. EVs in diseased states carry miRNAs and proteins reflective of the changed state of cells and can be endocytosed into other cells to exert regulatory effects. Thus, EVs represent a form of paracrine genetic regulation in health and disease. Myocardial infarction (MI) and pulmonary embolism (PE) are leading causes of cardiovascular deaths worldwide. It is important to develop new diagnostic and therapeutic approaches that could lead to medical advances. Circulating …

What Is The Additive Value Of Nutritional Deficiency To Va-Fi In The Risk Assessment For Heart Failure Patients?, Molly J Horstman, Christopher I Amos, Ariela Orkaby, Javad Razjouyan

Faculty, Staff and Student Publications

OBJECTIVES: to assess the impact of adding the Prognostic Nutritional Index (PNI) to the U.S. Veterans Health Administration frailty index (VA-FI) for the prediction of time-to-death and other clinical outcomes in Veterans hospitalized with Heart Failure.

METHODS: A retrospective cohort study of veterans hospitalized for heart failure (HF) from October 2015 to October 2018. Veterans ≥50 years with albumin and lymphocyte counts, needed to calculate the PNI, in the year prior to hospitalization were included. We defined malnutrition as PNI ≤43.6, based on the Youden index. VA-FI was calculated from the year prior to the hospitalization and identified three groups: …

Loss Of Tumor Suppressor Tmem127 Drives Ret-Mediated Transformation Through Disrupted Membrane Dynamics, Larissa C B Oliveira, Rebecca Cabral-Dias, Qianjin Guo, Patricia L M Dahia, Douglas S Richardson, Costin N Antonescu, Lois M Mulligan

Faculty, Staff and Student Publications

Internalization from the cell membrane and endosomal trafficking of receptor tyrosine kinases (RTKs) are important regulators of signaling in normal cells that can frequently be disrupted in cancer. The adrenal tumor pheochromocytoma (PCC) can be caused by activating mutations of the rearranged during transfection (RET) receptor tyrosine kinase, or inactivation of TMEM127, a transmembrane tumor suppressor implicated in trafficking of endosomal cargos. However, the role of aberrant receptor trafficking in PCC is not well understood. Here, we show that loss of TMEM127 causes wildtype RET protein accumulation on the cell surface, where increased receptor density facilitates constitutive ligand-independent activity and …

Alterations In Brain Morphometric Networks And Their Relationship With Memory Dysfunction In Patients With Type 2 Diabetes Mellitus, Chaewon Suh, Youngeun Shim, Hyeonji Lee, Yejin Kim, Jae-Hyoung Cho, Sujung Yoon, In Kyoon Lyoo

Faculty, Staff and Student Publications

Cognitive dysfunction, a significant complication of type 2 diabetes mellitus (T2DM), can potentially manifest even from the early stages of the disease. Despite evidence of global brain atrophy and related cognitive dysfunction in early-stage T2DM patients, specific regions vulnerable to these changes have not yet been identified. The study enrolled patients with T2DM of less than five years’ duration and without chronic complications (T2DM group, n=100) and demographically similar healthy controls (control group, n=50). High-resolution T1-weighted magnetic resonance imaging data were subjected to independent component analysis to identify structurally significant components indicative of morphometric networks. Within these networks, the groups’ …