Life Sciences Commons^™

Medial Temporal Lobe Surface Features And Cognition In Aging Amyloid-Positive Individuals, Jacob Johnston

Undergraduate Honors Theses

Memory consolidation and metabolism are known to differ between amyloid-beta plaque positive (A+) and negative (A-) amnestic Mild Cognitive Impairment (aMCI) and Alzheimer's disease (AD) dementia participants despite similar medial temporal lobe (MTL) volume between groups. Using high-dimensional surface analysis (shape characterization), this study identified structural differences in the medial temporal lobe between A+ aMCI, A+ AD dementia, and amyloid-negative (A-) healthy control groups (CON), specifically in the CA1 and subiculum regions of the hippocampus and entorhinal cortex. Additionally, regional brain surface-based features were correlated with procedural and episodic memory measures, finding positive associations between CA1 integrity and episodic memory …

Malnutrition Alters Protein Expression Of Kndy Neuropeptides In The Arcuate Nucleus Of Mature Ewes, Jennifer F. Thorson, Ligia D. Prezotto

Department of Animal Science: Faculty Publications

The neuropeptides kisspeptin, neurokinin B, and dynorphin A are imperative for the pulsatile secretion of gonadotropin-releasing hormone and luteinizing hormone to ultimately regulate reproductive cyclicity. A population of neurons co-expressing these neuropeptides, KNDy neurons, within the arcuate nucleus of the hypothalamus (ARC) are positioned to integrate energy status from afferent neuronal and glial cells. We hypothesized that KNDyexpressing neurons in the ARC of mature ewes are influenced by energy balance. To test this hypothesis, ovary-intact, mature ewes were fed to lose, maintain, or gain body weight and hypothalamic tissue harvested during the luteal phase of the estrous cycle. Fluorescent, multiplex …

A Study Assessing Stress Induction Methods And Regulation Genes, As Well As Cellular Ultrastructure And Automated Image Analysis In Synechocystis Sp. Pcc 6803, Rebekah White

Theses and Dissertations

In the world of microorganisms, cyanobacteria are a uniquely skilled powerhouse. They participate in global nutrient and carbon cycling. They can photosynthesize, which allows them to use sunlight and carbon dioxide to produce cellular energy. However, photosynthesis involves a lot of molecular interactions where environmental stresses can interfere, increasing the chances of oxidative stress and nutrient imbalances that can amplify deleterious effects. One such stress, iron limitation, is well-studied and its responses in the cell are known. This study assesses the differences between responses when induced by two different iron limitation methods. Then, a specific stress response regulator DspA, also …

A Library Of 39 Plant Peroxidases: Plants From The Families Cucurbitaceae And Brassicaceae Show Promising Peroxidase And Peroxygenase Activity, Sally Hess

Senior Honors Theses

Peroxidases are enzymes that reduce reactive oxygen species (ROS) and oxidize organic substances in living cells. The textile industry, wastewater treatment plants, immunological laboratories, and diagnostic laboratories have extensively used horseradish peroxidase for their oxidating needs. This research aims to provide a tool for identifying potentially useful sources of peroxidases in addition to specifically pinpointing a novel peroxygenase in jalapeño seeds. By using two widely accepted peroxidase assays, the guaiacol assay and the fluorophenol assay, fruits of the genus Cucurbita and roots or leaves of the family Brassicaceae were seen to have high peroxidase activity. Additionally, novel peroxygenase activity was …

Hell's Tails: Localizing The Tail Proteins Of The Hyperthermophile-Infecting Virus Ssv1, Jane Arterberry

University Honors Theses

The tail of Saccharolobus spindle-shaped virus 1 (SSV1) has long been thought to be composed solely of viral protein 4 (VP4), but recent SSV1 structural studies have implicated the presence of additional structural proteins C166 and B78 in SSV1's unique tail. The exact locations of these proteins have not been confirmed. Structural proteins VP4 (C-terminus) and B78 are hypothesized to contribute to an adapter region bridging the viral capsid to the tail, whereas C166 is thought to comprise the majority of an internal extended structure, respectively. SSV1 structural proteins lack cysteine residues providing targets to determine their locations in the …

Ecosystem Sentinels As Early-Warning Indicators In The Anthropocene, Elliott L. Hazen, Matthew S. Savoca, T.J. Clark-Wolf, Max Czapanskiy, Peter M. Rabinowitz, Briana Abrahms

Wildland Resources Faculty Publications

We are already experiencing the rapid pace of environmental perturbation in the Anthropocene, necessitating the development of new tools and techniques for measuring changes in ecosystem dynamics. Sentinel species, from birds to invertebrates, have been used to provide insights into ecosystem function, as leading indicators of risk to human health and as harbingers of future change, with implications for ecosystem structure and function. Here, we offer an update to previous research identifying marine top predators as indicators of ecosystem shifts and examine terrestrial sentinels and the latest research on sentinels of pollution and human health. Using ecosystem sentinels enables rapid …

Smokejumper Obituary: Marshall, Curtis A. (Redding 1968), National Smokejumper Association

Smokejumper Obituaries

No abstract provided.

Placental Co-Transcriptional Activator Vestigial-Like 1 (Vgll1) Drives Tumorigenesis Via Increasing Transcription Of Proliferation And Invasion Genes, Heather M Sonnemann, Barbara Pazdrak, Barbara Nassif, Yimo Sun, Lama Elzohary, Arjun S Katailiha, Gregory Lizée

Faculty, Staff and Student Publications

INTRODUCTION: Vestigial-like 1 (VGLL1) is a co-transcriptional activator that binds to TEA domain-containing transcription factors (TEADs). Its expression is upregulated in a variety of aggressive cancer types, including pancreatic and basal-like breast cancer, and increased transcription of VGLL1 is strongly correlated with poor prognosis and decreased overall patient survival. In normal tissues, VGLL1 is most highly expressed within placental trophoblast cells, which share the common attributes of rapid cellular proliferation and invasion with tumor cells. The impact of VGLL1 in cancer has not been fully elucidated and no VGLL1-targeted therapy currently exists.

METHODS: The aim of this study was to …

Defining The Kras- And Erk-Dependent Transcriptome In Kras-Mutant Cancers, Jeffrey A Klomp, Jennifer E Klomp, Clint A Stalnecker, Kirsten L Bryant, A Cole Edwards, Priya S Hibshman, Ye S Lee, Alexis J Morales, Khalilah E Taylor, Sen Peng, Nhan L Tran, Laura E Herring, Alex W Prevatte, Natalie K Barker, Laura D Hover, Jill Hallin, Alexey Sorokin, Preeti Marie Kanikarla, Saikat Chowdhury, Oluwadara Coker, Hey Min Lee, Craig M Goodwin, Prson Gautam, Peter Olson, James G Christensen, John P Shen, Scott Kopetz, Lee M Graves, Kian-Huat Lim, Andrea Wang-Gillam, Krister Wennerberg, Adrienne D Cox, Channing J Der

Faculty, Staff and Student Publications

How the KRAS oncogene drives cancer growth remains poorly understood. Therefore, we established a systemwide portrait of KRAS- and ERK-dependent gene transcription in KRAS-mutant cancer to delineate the molecular mechanisms of growth and of inhibitor resistance. Unexpectedly, our KRAS-dependent gene signature diverges significantly from the frequently cited Hallmark KRAS signaling gene signature, is driven predominantly through the ERK mitogen-activated protein kinase (MAPK) cascade, and accurately reflects KRAS- and ERK-regulated gene transcription in KRAS-mutant cancer patients. Integration with our ERK-regulated phospho- and total proteome highlights ERK deregulation of the anaphase promoting complex/cyclosome and other components of the cell cycle machinery as …

Impact Of Risk-Based Therapy On Late Morbidity And Mortality In Neuroblastoma Survivors: A Report From The Childhood Cancer Survivor Study, Danielle Novetsky Friedman, Pamela J Goodman, Wendy M Leisenring, Lisa R Diller, Susan L Cohn, Susan A Smith, Suzanne L Wolden, Joseph P Neglia, Kirsten K Ness, Todd M Gibson, Paul C Nathan, Lucie M Turcotte, Brent R Weil, Leslie L Robison, Kevin C Oeffinger, Gregory T Armstrong, Charles A Sklar, Tara O Henderson

Faculty, Staff and Student Publications

Background: Early efforts at risk-adapted therapy for neuroblastoma are predicted to result in differential late effects; the magnitude of these differences has not been well described.

Methods: Late mortality, subsequent malignant neoplasms (SMNs), and severe/life-threatening chronic health conditions (CHCs), graded according to CTCAE v4.03, were assessed among 5-year Childhood Cancer Survivor Study (CCSS) survivors of neuroblastoma diagnosed 1987-1999. Using age, stage at diagnosis, and treatment, survivors were classified into risk groups (low [n = 425]; intermediate [n = 252]; high [n = 245]). Standardized mortality ratios (SMRs) and standardized incidence ratios (SIRs) of SMNs were compared with matched population controls. …

Vertebral Hu Value And The Pectoral Muscle Index Based On Chest Ct Can Be Used To Opportunistically Screen For Osteoporosis, Xiong-Yi Wang, Sheng Pan, Wei-Feng Liu, Yi-Ke Wang, Si-Min Yun

Faculty, Staff and Student Publications

BACKGROUND: Existing studies have shown that computed tomography (CT) attenuation and skeletal muscle tissue are strongly associated with osteoporosis; however, few studies have examined whether vertebral HU values and the pectoral muscle index (PMI) measured at the level of the 4th thoracic vertebra (T4) are strongly associated with bone mineral density (BMD). In this study, we demonstrate that vertebral HU values and the PMI based on chest CT can be used to opportunistically screen for osteoporosis and reduce fracture risk through prompt treatment.

METHODS: We retrospectively evaluated 1000 patients who underwent chest CT and DXA scans from August 2020-2022. The …

Association Of Differential Censoring With Survival And Suboptimal Control Arms Among Oncology Clinical Trials, Eric J Hsu, Timothy A Lin, Dor R Dabush, Zachary Mccaw, Alex Koong, Joseph Abi Jaoude, Ramez Kouzy, Molly B El Alam, Sonal Noticewala, Yumeng Yang, Alexander D Sherry, Clifton D Fuller, Charles R Thomas, Chad Tang, Pavlos Msaouel, Prajnan Das, Bo Huang, Lu Tian, Ryan Sun, J Jack Lee, Tomer Meirson, Ethan B Ludmir

Faculty, Staff and Student Publications

Differential censoring, which refers to censoring imbalance between treatment arms, may bias the interpretation of survival outcomes in clinical trials. In 146 phase III oncology trials with statistically significant time-to-event surrogate primary endpoints, we evaluated the association between differential censoring in the surrogate primary endpoints, control arm adequacy, and the subsequent statistical significance of overall survival results. Twenty-four (16%) trials exhibited differential censoring that favored the control arm, whereas 15 (10%) exhibited differential censoring that favored the experimental arm. Positive overall survival was more common in control arm differential censoring trials (63%) than in trials without differential censoring (37%) or …

How Context Links To Best Practice Use In Long-Term Care Homes: A Mixed Methods Study, Yinfei Duan, Jing Wang, Holly J Lanham, Whitney Berta, Stephanie A Chamberlain, Katharina Choroschun, Yuting Song, Janelle Santos Perez, Shovana Shrestha, Anna Beeber, Ruth A Anderson, Leslie Hayduk, Greta G Cummings, Peter G Norton, Carole A Estabrooks

Faculty, Staff and Student Publications

Background: Context (work environment) plays a crucial role in implementing evidence-based best practices within health care settings. Context is multi-faceted and its complex relationship with best practice use by care aides in long-term care (LTC) homes are understudied. This study used an innovative approach to investigate how context elements interrelate and influence best practice use by LTC care aides.

Methods: In this secondary analysis study, we combined coincidence analysis (a configurational comparative method) and qualitative analysis to examine data collected through the Translating Research in Elder Care (TREC) program. Coincidence analysis of clinical microsystem (care unit)-level data aggregated from a …

Improved Liver Disease Prediction From Clinical Data Through An Evaluation Of Ensemble Learning Approaches

Faculty, Staff and Student Publications

PURPOSE: Liver disease causes two million deaths annually, accounting for 4% of all deaths globally. Prediction or early detection of the disease via machine learning algorithms on large clinical data have become promising and potentially powerful, but such methods often have some limitations due to the complexity of the data. In this regard, ensemble learning has shown promising results. There is an urgent need to evaluate different algorithms and then suggest a robust ensemble algorithm in liver disease prediction.

METHOD: Three ensemble approaches with nine algorithms are evaluated on a large dataset of liver patients comprising 30,691 samples with 11 …

Looking For The Oldest Diatoms, Karolina Brylka, Sylvain Richoz, Andrew J. Alverson, David J. Conley

Biological Sciences Faculty Publications and Presentations

Paleontological observations of ancient flora and fauna provide powerful insights into past diversity and relationship dynamics between organisms and their environments. Diatoms are globally distributed protists that influence major biogeochemical cycles and sustain oceanic food webs. The fossil diatom record extends 120 million years back to the Early Cretaceous where rare deposits were discovered worldwide and are occasionally represented by diverse communities. However scarce, the taxonomic richness and geographical spread of these diatom communities suggest prior evolutionary events and therefore earlier deposits. To complement the existing fossil information and to discover diatom deposits predating 120 Ma, we examined 33 study …

The Impact Of Virtual Reality Training On Women’S Collegiate Tennis In Physical And Mental Performance, Megan Leblanc, Jordan Schools

CSKLS Annual Conference

This presentation focuses on the use of virtual reality (VR) in mental preparation among collegiate tennis players. In addition it explores the ways that VR and EEG can be used to enhance feeling of preparedness and prime the mind for performance.

Immersive Training: Elevating Athlete Performance And Cognitive Focus With Vr And Eeg Integration, Calvin Lopez, Jordan Schools

CSKLS Annual Conference

The pursuit of optimal athletic performance and mental focus has long been a central focus in sports science research. In recent years, the integration of virtual reality (VR) technology has emerged as a promising avenue for enhancing athlete development. This abstract presents findings from a case study investigating the efficacy of using Meta Quest 2 VR headsets coupled with EEG headbands to track and improve focus and mental preparedness levels in athletes. The study employed a case study methodology, focusing on the use of VR technology to enhance athlete performance and mental focus. Specifically, the research aimed to assess the …

The Anticancer Activity Of Novel 8-Hydroxyquinoline Derivatives Against 5-Fluorouracil Resistant Colorectal Cancer Cells, Mazoun Talal Al Azzani

Thesis/ Dissertation Defenses

Cancer is a global health crisis for which 20 million new cases were diagnosed in 2022 alone, with 9.3 million cancer-related deaths. Nearly 900,000 deaths were due to colorectal cancer (CRC), which is the third leading cause of cancer related mortality. In the United Arab Emirates, 550 new cases of CRC were reported in 2022. A major clinical challenge with CRC treatment is the development of resistance to 5-fluorouracil (5FU)-based chemotherapy regimens, which are the standard treatment for CRC. In the present study, the anticancer activity of two novel 8-hydroxyquinoline (8-HQ) derivatives AS45 and AS47 was examined against 5FU-sensitive (HCT116) …

Cultivating Gratitude In Christian Sport Professionals, Julie Powell

CSKLS Annual Conference

Using the framework of the eight dimensions of wellness and the five habits of a flourishing life, this presentation will focus on how to cultivate the virtue of gratitude and thanksgiving in order to combat the undesirable vice of entitlement. This presentation will outline what gratitude is, how we express it, and how to embody it as a part of our character. Cultivating a grateful disposition can be the result of practicing spiritual disciplines and the session will brainstorm ideas of how this might look in our current profession and in our personal lives.

Tough Conversations In The Classroom, Dale Connally, David Waddell

CSKLS Annual Conference

There are quite a few topics of discussion that can be difficult within the curricula for Recreation and Leisure, Health Professions, Sport and other disciplines within CSKLS. These might include environmental stewardship, immigration, political funding for programs, LGTBQ matters, racial discrimination, and DEI. We will explore some approaches gleaned from secular pedagogy sources. We will present useful techniques for planning discussions, facilitating, and following up after discussion. We will discuss differences between teaching at a public institution and a faith-based institution. The presenters will give a couple of examples from their classes for discussion. Attendees will be encouraged to engage …

Physiological And Biochemical Responses Of Maize (Zea Mays L.) Cultivars Under Salinity Stress, Nosheen Iftikhar, Shagufta Perveen, Baber Ali, Muhammad Hamzah Saleem, Mohammad Khalid Al-Sadoon

Turkish Journal of Agriculture and Forestry

Salt stress is a serious threatening factor for cereal crops such as maize (Zea mays L.) by affecting their growth and development. In the current era, the requirement for staple crops is increasing, so it is important to screen out salt-tolerant genotypes. For this purpose, a pot experiment was designed within three replications on ten different genotypes of the maize. The plants were planted in plastic pots and salt stress (0, 40, 70, 100 mM) was maintained. The salt stress induced a noticeable reduction in plant growth traits (shoot length, root length, shoot, root fresh and dry weight, and leaf …

Comparative Analysis Of Closed Hydroponic Systems And Planting Seasons For Lettuces, Demet Çeki̇n, Mostafa Hassanen, Mariam Hassanen, Nayira Hassan, Ricarda Lothmann, Hani Sewilam

Turkish Journal of Agriculture and Forestry

Hydroponic cultivation techniques offer innovative solutions to address challenges in conventional agriculture, such as soil infertility, disease outbreaks, and poor drainage. This study focuses on comparing the performance of different closed hydroponic systems for lettuce cultivation across distinct winter and summer planting seasons. Four closed hydroponic systems—Deep Water Culture (DWC), Nutrient Film Technique (NFT), Media-bed system (MB), and Sandponic (SP)—were evaluated for their impact on vegetative growth parameters, leaf nutrient content, chlorophyll and carotene levels, and water consumption. Our findings reveal that the DWC and NFT systems exhibited greater yield increases in winter (81.90% and 75.64%, respectively) and in summer …

Evaluation Of Arginine Decarboxylase (Fvadc) And Spermidine Synthase (Fvspds) Genes Of Woodland Strawberry (Fragaria Vesca L.) In Nicotiana Tabacum, Ákos Mendel, Erzsebet Kiss

Turkish Journal of Agriculture and Forestry

A comparative analysis was performed under in vitro conditions using Nicotiana tabacum lines overexpressing the genes of arginine decarboxylase (FvADC) and spermidine synthase (FvSPDS) enzymes. The transgenic and native lines were tested under controlled conditions and exposed to long-term treatment of arginine (150 mg/L), putrescine (10 mg/L), and spermidine (10 mg/L). Chlorophyll and lignin contents of the samples were measured spectrophotometrically, while proline, putrescine, spermidine, and spermine contents were determined using high-performance liquid chromatography methods. The experimental results showed that the arginine decarboxylase enzyme affects polyamine metabolism. As it is involved in several other biosynthetic pathways, this effect is significant …

Local Organic And Inorganic Amendments To Improve Table Grape Production In Mediterranean Vineyards Of Southern Türkiye, Semi̇h Tangolar, Simone Priori, Serpi̇l Tangolar, Meti̇n Turan, Erhan Akça

Turkish Journal of Agriculture and Forestry

In the Mediterranean Region, calcareous soils are generally considered to be of low fertility. The use of large amounts of chemicals and irrigation to compensate for low productivity reduces the producer’s net profit and degrades physical and chemical properties of the soils. As a result, the selection of adaptable genotypes is important in agriculturally limited lands. Grapevine is one of the leading crops in the Mediterranean Basin due to its high tolerance to abiotic conditions. However, the fact that the grapevine grows in extreme soils does not mean that such crop does not respond to a good nutritional program, especially …

Using Computational Modeling To Design Antiviral Strategies And Understand Plant-Virus Interactions, Hira Kamal, Muhammad Mubashar Zafar, Abdul Razzaq, Aqsa Ijaz, Zunaira Anvar, Hayat Topçu, Khalid M. Elhindi, Asif Saeed, Urooj Fatima, Xuefei Jiang

Turkish Journal of Agriculture and Forestry

Using a bioinformatics approach to identify binding pockets between proteins is a preferable method before modifying the genome to delineate host interactions with viruses. Based on extensive proteomics data in numerous databases, several interaction prediction methods are available to identify binding sites between viruses and hosts at the individual residue level, but little is known about the interaction prediction strategy for plant viruses. Begomoviruses, belonging to the family Geminiviridae, constitute a group of circular single-stranded (ss) DNA viruses that encode multifunctional proteins responsible for viral replication, causing severe diseases in multiple host plants. These viruses usually escape through plant defense …

Eco-Friendly Management Of Helicoverpa Armigera Using Entomopathogenic Fungi: A Sustainable Approach, Hafiz Husnain Nawaz, Gülşah Kekli̇k, Yamin Bibi, Javed Iqbal, Attiq Ur Rehman, Mubashar Hussain, Shahzad Toufeeq, Rashid Azad, Khalid Ali Khan, Abid Farid, Abdul Qayyum

Turkish Journal of Agriculture and Forestry

Helicoverpa armigera Hübner, 1808 (Noctuidae; Lepidoptera) is one of the major pest problems causing production constraints in both conventional and organic agricultural systems. Chemical and semichemical, along with biotechnological approaches, like the sterile insect technique, are used to manage pest populations. However, these methods raise environmental concerns. Therefore, the current study was undertaken to assess the biocontrol potential of the entomopathogenic fungal strains Beauveria bassiana, Metarhizium anisopliae, Akanthomyces lecanii, and Purpureocillium lilacinus against H. armigera. These strains, identified through morphological and molecular techniques, were evaluated for their effectiveness in controlling H. armigera. A total of eight fungal strains were identified …

Differences In Farmers’ Market Patronage Among Participants In The Special Supplemental Nutrition Program For Women, Infants, And Children (Wic), Renata Blumberg, Emily Fowler, Gabrielle Guerrero, Yeon Bai, Meena Mahadevan, Pankaj Lal

Department of Nutrition and Food Studies Scholarship and Creative Works

In the United States, there is a high prevalence of diet-related diseases in low-income communities that lack access to fresh produce. Greater access to local farmers’ markets may affect individual and household intake of fruits and vegetables. However, barriers to farmers’ market access remain. The purpose of this study is to evaluate the differences between participants in the Special Supplemental Nutrition Program for Women, Infants, and Children (WIC) who regularly shop at farmers’ markets and those who do not. We found that regular farmers’ market shoppers tended to consume on average more fruits and vegetables than WIC participants who did …

Deferred Grazing And Sheep At The Break Of Season In South West Western Australia, Department Of Primary Industries And Regional Development, Western Australia

Animal production and livestock factsheets

Grazing annual pastures in the first 12 days after germination can lower the productivity of pastures during winter by removing poorly rooted plants, and by reducing plant leaf area to sub-optimal levels. The Department of Primary Industries and Regional Development (DPIRD) recommends deferring grazing of pasture paddocks at the break-of-season until feed on offer is at least 500–800 kilograms of dry matter per hectare (kg DM/ha). At this level, pasture plants are likely to be firmly rooted and have sufficient leaf area to be productively grazed.

Soil And Organic Carbon Losses By Water Erosion In Coffee-Growing Areas In Southern Minas Gerais, Brazil, Derielsen Brandão Santana, Guilherme Da Silva Rios, Guilherme Henrique Expedito Lense, Felipe Gomes Rubira, Flávio Roberto Araújo Franceschi, Velibor Spalevic, Joaquim Ernesto Bernardes Ayer, Ronaldo Luiz Mincato

Turkish Journal of Agriculture and Forestry

Organic carbon performs essential functions in soils. Soils act as sources or sinks of atmospheric organic carbon. Agricultural management influences soil organic carbon, impacting climate change. One of the crops most vulnerable to climate change is coffee. Brazil is the world's largest coffee producer, with a predominance of management under a conventional system, with sloping terrain and the absence of conservationist practices. The absence of conservationist practices results in an increase in soil loss rates due to water management and carbon emissions, as well as a reduction in coffee production. This paper aimed to estimate soil and organic carbon losses …

Effects Of Nutrient Solution Strength, Pgpb, And Mycorrhizal Inoculation On Growth,Yield, And Quality Of Strawberry, Masoud Haghshenas, Sander H. Van Delden, Mohammad Javad Nazarideljou

Turkish Journal of Agriculture and Forestry

Strawberry (Fragaria × ananassa Duch) cultivation is not without difficulty since its root environment is sensitive to many biotic and abiotic stresses. In addition, production is often phosphate limited, even at relatively high phosphorus levels, as the high fruit yields greatly exceed the capacity of the phosphorus supply by the root system. Arbuscular mycorrhizal fungi (AMF), plant growth promoting bacteria (PGPB), and combinations of both are known to mitigate stress and enhance nutrient uptake, particularly phosphorus. Therefore, we studied the effects of AMF (Glomus mosseae, and Glomus intraradices), PGPB (Azospirillum lipoferum DSM1691, and Pseudomonas fluorescens DSM 50090), and their combinations …