Life Sciences Commons^™

Nella Frierson Full Interview Transcript, Urban Green Lab, Tennessee State University

Hear from Nella "Ms. Pearl" Frierson - Nashvillian in Community Gardening

No abstract provided.

Feather Mites Through Genomic And Ecological Lenses: Host Specificity And Symbiosis At Multiple Levels Of Organization, Alix Elizabeth Matthews

Student Theses and Dissertations

Symbionts, or organisms that form close and persistent associations with hosts, are ubiquitous across the tree of life, and symbioses are among the most common interspecific interactions in the natural world. Symbionts play a fundamental ecological and evolutionary role across multiple levels of biological organization – from individuals to ecosystems – and can have strong effects on global biodiversity. However, many aspects of these relationships are poorly understood including symbiont genetic diversity (inter- and intraspecific), host specificity, and the mechanisms underlying their functional nature or any context-dependent patterns. This dissertation uses a model symbiosis between permanent, obligate, ectosymbiotic vane-dwelling feather …

Correlation Between Pass Speed, Leg Strength And Explosive Leg Performance In Male Soccer Players, Keigo Kobayashi

Student Theses and Dissertations

This study examined correlation between inside pass speed and performance and descriptive variables among male soccer players. Eighteen players from a University men’s soccer club team completed performance tasks and a lower body muscular strength task. Descriptive data (age, weight, height, body mass index, body fat percentage and years of experience) were also collected. Pearson correlation examined relationships between pass speed and each performance and descriptive variable. Average pass speed was 81.24 ± 12.71 km/h. Only the 4-counter movement jump height and sprint were significantly correlated to pass speed. No relationship was found between the other performance tasks and any …

2024 Nebraska Agricultural Custom Rates With Statewide Survey Summary, Glennis Mcclure

Cornhusker Economics

The 2024 University of Nebraska-Lincoln Custom Rates Survey indicates that rate increases are the norm since the latest survey was completed in 2022. Table 1 provides a summary and percentage change for several common custom operations from 2022 to 2024. Custom field operations such as drilling and planting show increases in 2024 from seven to twenty-seven percent and harvesting rates for various crops indicate state average rate increases from fourteen to twenty-three percent, with the irrigated corn harvest now at $47.84 per acre for the state average. Other custom services, such as spraying for weed control using a self-propelled sprayer, …

Selecting Fertilizer Mixtures For Fertigation, Bhupinder Jatana, Tom Grayson Sanders Iii

Horticulture

Depending upon the physio-chemical properties of the fertilizer solution, many fertilizers, both solid and liquid, are suitable for fertigation (application of fertilizer dissolved in irrigation water). However, some fertilizer materials are incompatible with concentrated solutions, can react with minerals in irrigation water, or with pesticides in tank mixes, and form insoluble compounds and precipitates. Precipitation of fertilizers renders them unavailable for plant uptake and could further clog the micro-irrigation systems. This article talks about fertilizers, fertilizer formulations, fertilizer compatibility, and criteria for selecting fertilizer mixtures for fertigation. This information will be helpful for growers utilizing fertigation as a means to …

Functional Studies Of Dcaf14 In Replication Fork Stability And Genome Integrity, Neysha Tirado-Class

USF Tampa Graduate Theses and Dissertations

The response to replication stress is critical for ensuring the stability of replication forks and preserving genome integrity. To aid this response, several factors are recruited to stalled replication forks to prevent irreversible collapse of the replication machinery. Replication fork proteomic analyses of stalled forks reveal enrichment of DCAF14, a component of the Cullin4-RING (CRL4) E3 ubiquitin ligase complex, that mediates replication fork protection by blocking nuclease-mediated digestion of nascent DNA. However, little is known about the molecular mechanism by which DCAF14 functions in the context of replication stress. Notably, loss of function mutations in DCAF14 are associated with a …

Aerobic And Anaerobic Exercise's Impact On Cognitive Functions In Eighth Grade Students., Heather Erwin, Sarah Schreiber

Kinesiology and Health Promotion Faculty Publications

The present study examined the impact of aerobic and anaerobic exercise on cognitive abilities in eighth grade students at one middle school in southeastern US. It is known that youth do not meet recommended physical activity levels, yet there is research demonstrating a clear connection between aerobic exercise and academic performance. There is a gap in evidence regarding anaerobic exercise and cognitive capabilities. If professionals know which type of exercise is most beneficial for enhancing cognition in youth, it will behoove them to incorporate these practices in physical education and other areas throughout the school day for students. Thus, this …

Group B Streptococcus Intestinal Barrier Disruption And The Protective Role Of Butyrate, Kristen Dominguez

USF Tampa Graduate Theses and Dissertations

Group B Streptococcus (Streptococcus agalactiae; GBS) is a leading cause of neonatal sepsis worldwide. Intrapartum antibiotics in GBS-colonized mothers significantly reduced the incidence of early-onset sepsis occurring in the first week of life; however, there are currently no prophylactic interventions for late-onset sepsis, occurring within the first 3 months of life. GBS is a pathobiont that primarily colonizes the gastrointestinal tract and can cross the intestinal barrier via both the paracellular and transcellular routes, causing invasive disease. Neonatal susceptibility to GBS intestinal translocation is mediated by inadequate epithelial barrier function and microbiome immaturity; however, the mechanisms by which GBS exploits …

O-Demethyl Galantamine Alters Protein Expression In Cerebellum Of 5xfad Mice, İrem Kiriş, Merve Karayel Başar, Büşra Gurel, Tomasz Mroczek, Ahmet Tarik Baykal

Turkish Journal of Biology

Background/aim: Alzheimer’s disease (AD), one of the most common health issues, is characterized by memory loss, severe behavioral disorders, and eventually death. Despite many studies, there are still no drugs that can treat AD or stop it from progressing. Previous in vitro tests showed that O-demethyl galantamine (ODG) might have a therapeutic potential owing to its 10 times higher acetylcholinesterase inhibitory activity than galantamine (GAL). Materials and methods: We aimed to assess effect of ODG on a molecular level 12-month-old 5xFAD Alzheimer’s mouse model. To this end, following the administrations of ODG and GAL, used as a positive control, protein …

Petox-Dope Nanoliposomes Functionalized With Peptide 563 In Targeted Bikdda Delivery To Prostate Cancer, Ayca Ece Nezi̇r, Zeynep Büşra Bolat, Ongun Mehmet Saka, Itir Ebru Zemheri̇, Sevgi̇ Gülyüz, Umut Uğur Özköse, Özgür Yilmaz, Asuman Bozkir, Fi̇kretti̇n Şahi̇n, Di̇lek Telci̇

Turkish Journal of Biology

BackgroundNanocarrier-based systems have cultivated significant improvements in prostate cancer therapy. However, the efforts are still limited in clinical applicability, and more research is required for the development of effective strategies. Here, we describe a novel nanoliposomal system for targeted apoptotic gene delivery to prostate cancer.MethodsPoly (2-ethyl-2-oxazoline) (PEtOx) dioleoyl phosphatidylethanolamine (DOPE) nanoliposomes were conjugated with the prostate-specific membrane antigen (PSMA)-targeting peptide GRFLTGGTGRLLRIS (P563) and loaded with BikDDA, a mutant form of the proapoptotik Bik. We selected 22Rv1 cells with moderate upregulation of PSMA to test the in vitro uptake, cell death, and in vivo anti-cancer activity of our formulation, P563-PEtOx-DOPE-BikDDA.ResultsBikDDA was …

The Apoptotic Effect Of Garlic (Allium Sativum) Derived Sevs On Different Types Of Cancer Cell Lines In Vitro, Naz Ünsal, Polen Koçak Deni̇zci̇, Hazal Yilmaz, Fi̇kretti̇n Şahi̇n, Merve Yildirim Canpolat

Turkish Journal of Biology

Small extracellular vesicles (SEVs) are known to have an impact on the physiological circumstances of the target cells, are a critical component of cell-to-cell communication and have been implicated in a variety of diseases. Although it has been proposed that edible plant-derived nanoparticles have effect on communication with mammalian cells, the influence of these nanoparticles on cancer cell development has yet to be explored. In order to characterize small extracellular vesicles obtained from garlic, specific SEV surface markers and antibodies and size detections were performed by using scanning electron microscopy and nanoparticle tracking analysis. Human hepatoma, Hep3B, human neuroblastoma SH-SY5Y, …

Proteomic Investigation Of Acute And Chronic Hypoxia /Reoxygenation Responsive Proteins And Pathways In H9c2 Cardiomyoblasts, Merve Öztuğ, Evren Kilinç, Zeynep A. Öztuğ Durer, Emel Baloğlu

Turkish Journal of Biology

Background/Aim: Ischemic heart diseases continue to be a significant global cardiovascular problem in today's world. Myocardial reperfusion (R) is provided with an effective and rapid treatment; however, it can lead to fatal results as well as ischemia (I). This study aims to use proteomic analysis to assess proteins and pathways in H9C2 cardiomyoblast cells exposed to hypoxic conditions, followed by reoxygenation, representing I /R injury for both short and long terms, reflecting acute and chronic hypoxia, respectively. Utilizing advanced techniques, our goal is to identify and characterize key proteins undergoing alterations during these critical phases.Materials and methods: H9C2 cardiomyoblasts, commonly …

Lungbank: A Novel Biorepository Strategy Tailored For Comprehensive Multi-Omics Analysis And P-Medicine Applications In Lung Cancer, Di̇lek Demi̇rcan Çeker, Volkan Baysungur, Serdar Evman, İlker Kolbaş, Abdurrahi̇m Gördebi̇l, Si̇nem M. Nalbantoğlu, Yusuf Tambağ, Ömer Kaçar, Ahmet Mi̇di̇, Hati̇ce Aslanoğlu, Nülüfer Kara, Ni̇lgün Algan, Ayberk Boyacioğlu, Betül Karademi̇r Yilmaz, Ali̇ Şahi̇n, Hi̇vda Ülbeği̇ Polat, Abi̇di̇n Şehi̇toğullari, Ali̇ Osman Çibikdi̇ken, Mücahi̇t Büyükyilmaz, İbrahi̇m Berkan Aydi̇lek, Abdulkeri̇m Eneş, Sevde Küçüker, Fati̇h Karakaya, İhsan Boyaci, Mahmut Gümüş, Onur Şenol, Merve Öztuğ, Evren Saban, Ömer Soysal, Nur Büyükpinarbaşili, Akif Turna, Mehmet Zeki Günlüoğlu, Aslı Çakir, Şaban Teki̇n, Uygar Tazebay, Abdullah Karadağ

Turkish Journal of Biology

Background/aim: LUNGBANK was established as a pioneering biorepository dedicated to lung cancer. Designed for streamlined biospecimens handling, it employs high-volume technologies to secure high-quality samples. Fortified with robust data management, LUNGBANK efficiently integrates diverse datasets. This strategic infrastructure positions LUNGBANK as more than a biospecimens repository; it operates as a sophisticated platform for advanced lung cancer research, amplifying the potential for meaningful discoveries.Materials and methods: LUNGBANK is intricately designed to streamline the collection, transfer, storage, and processing of biospecimens, employing high-volume technologies to ensure the acquisition of high-quality biological specimens. The system is fortified with robust data management protocols, facilitating …

Metagenomic Analysis Of Antarctic Microbial Communities, David Michael Robinson

Biology ETDs

The research presented in this dissertation focuses on microbial roles in biogeochemical cycling in Antarctic aquatic environments. The major objective of my research was to examine the impact of biotic and abiotic pressures on nutrient cycling in microbially dominated systems. I used three perennially ice covered lakes in the McMurdo Dry Valleys (MDVs) along with subglacial lakes Whillans and Mercer as natural case studies. The MDVs are located in Victoria Land, East Antarctica and have been studied since 1993 as part of the McMurdo Long Term Ecological Research Project. Viruses in the MDVs were shown to be seasonally abundant and …

Mettl3-Mediated Chromatin Contacts Promote Stress Granule Phase Separation Through Metabolic Reprogramming During Senescence, Chen Wang, Hideki Tanizawa, Connor Hill, Aaron Havas, Qiang Zhang, Xue Hao, Lu Wang, Hao Nie, Yuan Qi, Bin Tian, Alessandro Gardini, Andrew V Kossenkov, Aaron Goldman, Shelley L Berger, Ken-Ichi Noma, Peter D Adams, Rugang Zhang

Faculty, Staff and Student Publications

METTL3 is the catalytic subunit of the methyltransferase complex, which mediates m6A modification to regulate gene expression. In addition, METTL3 regulates transcription in an enzymatic activity-independent manner by driving changes in high-order chromatin structure. However, how these functions of the methyltransferase complex are coordinated remains unknown. Here we show that the methyltransferase complex coordinates its enzymatic activity-dependent and independent functions to regulate cellular senescence, a state of stable cell growth arrest. Specifically, METTL3-mediated chromatin loops induce Hexokinase 2 expression through the three-dimensional chromatin organization during senescence. Elevated Hexokinase 2 expression subsequently promotes liquid-liquid phase separation, manifesting as stress granule phase …

Contribution Of Matrix Metalloproteinase-2 And Matrix Metalloproteinase-9 To Upper Tract Urothelial Cancer Risk In Taiwan, Bo-Ren Wang, Hung-Huan Ma, Chao-Hsiang Chang, Cheng-Hsi Liao, Wen-Shin Chang, Ya-Chen Yang, Da-Tian Bau, Chia-Wen Tsai

Faculty, Staff and Student Publications

Matrix metalloproteinase (MMP)-2 and -9, which degrade type IV collagen, are linked to cancer invasion and metastasis. Gene polymorphisms in MMP-2 and MMP-9 can influence their function, impacting cancer development and progression. This study analyzed the association between polymorphisms MMP-2 rs243865 (C-1306T), rs2285053 (C-735T), and MMP-9 rs3918242 (C-1562T) with serum concentrations of these enzymes in upper tract urothelial cancer (UTUC) patients. We conducted a case-control study with 218 UTUC patients and 580 healthy individuals in Taiwan. Genotyping was performed using PCR/RFLP on DNA from blood samples, and MMP-2 and MMP-9 serum levels and mRNA expressions in 30 UTUC patients were …

Identifying Sudden Cardiac Arrest Risk In Adolescent Male Athletes, Alexis Sattler, Susan Rozzi

International Journal of Exercise Science

International Journal of Exercise Science 17(2): 874-886, 2024. The purpose of the study was to determine the prevalence of sudden cardiac arrest (SCA) risk factors in high school (HS) athletes. Thirty-three male soccer players from a public HS in the southeastern United States (ages 14-17) self-reported survey data. Participants reported demographic, lifestyle, heart health, COVID-19 history, and symptoms indicative of SCA risk. An electrocardiogram (ECG) assessed heart rate (HR), rhythm, and electrical activity. Resting HR and blood pressure (BP) were measured with a BP monitor. The association between COVID-19 history, HR, and BP were calculated. Participants were divided into ECG …

Beneficial Plant-Microbe Interactions And Stress Tolerance In Maize, Saroj Burlakoti, Ananta R. Devkota, Shital Poudyal, Amita Kaundal

Plants, Soils, and Climate Faculty Publications

Beneficial microbes are crucial for improving crop adaptation and growth under various stresses. They enhance nutrient uptake, improve plant immune responses, and help plants tolerate stresses like drought, salinity, and heat. The yield potential of any crop is significantly influenced by its associated microbiomes and their potential to improve growth under different stressful environments. Therefore, it is crucial and exciting to understand the mechanisms of plant-microbe interactions. Maize (Zea mays L.) is one of the primary staple foods worldwide, in addition to wheat and rice. Maize is also an industrial crop globally, contributing 83% of its production for …

A Model Of Time-Dependent Macromolecular And Elemental Composition Of Phytoplankton, Anne Willem Omta

Faculty Scholarship

Phytoplankton Chl:C:N:P ratios are important from both an ecological and a biogeochemical perspective. We show that these elemental ratios can be represented by a phytoplankton physiological model of low complexity that includes major cellular macromolecular pools. In particular, our model resolves time-dependent intracellular pools of chlorophyll, proteins, nucleic acids, carbohydrates/lipids, and N and P storage. Batch culture data for two diatom and two prasinophyte species are used to constrain parameters that represent specific allocation traits and strategies. A key novelty is the simultaneous estimation of physiological parameters for two phytoplankton groups of such different sizes. The number of free parameters …

Salt Pollution Reduces Turbidity, Dissolved Organic Matter, And Cyanobacteria In Experimental Vernal Pool Communities, Jared P. Vigil, Matthew S. Schuler

Department of Biology Faculty Scholarship and Creative Works

Anthropogenic activities such as the over-application of road deicers are causing an increase in the concentration of salts in historically fresh waters. Experimental and field investigations demonstrate that freshwater salinization disrupts ecosystem functions and services, causing the death of freshwater organisms and changes to nutrient conditions. Wetland habitats are one system negatively affected by salt pollution, including ephemeral wetlands (vernal pools) that fill with salt-polluted water after snowmelt. In urbanized areas, the degradation of these ecosystems could result in irreversible ecological damage including reduced water quality and a reduction in biodiversity. To investigate the effects of freshwater salinization on vernal …

Porphyrin Overdrive In Cancer: Unraveling The Mechanisms And Therapeutic Potential, Pravin D. Meshram

USF Tampa Graduate Theses and Dissertations

The increased understanding of cancer cell metabolism has shown options for novel treatment strategies. This thesis looks at the role of heme metabolism, specifically "porphyrin overdrive," in cancer progression and treatment. Cancer cells are hypermetabolic and rely on heme synthesis. When treated with δ-aminolevulinic acid (ALA), they abnormally accumulate heme intermediates. This metabolic weakness presents an opportunity to exploit heme synthesis in targeted cancer therapy. In this study, we suggest a "Bait and Kill" technique that uses exogenous ALA to induce protoporphyrin IX (PpIX) buildup in cancer cells, making them more susceptible to oxidative stress. We investigate the possibility of …

Limonoids Isolated From Chisocheton Pentandrus (Meliaceae) Stembarks And Its Cytotoxicity Towards Mcf-7 Breast Cancer Cell Line, Dudi Runadi, Intan Hawina Anjari, Purnama Purnama, Nurlelasari Nurlelasari, Desi Harneti, Tri Mayanti, Harizon Harizon, Ace Tatang Hidayat, Supriatno Salam, Mohamad Nurul Azmi, Unang Supratman

Makara Journal of Science

Chisocheton is a large source of limonoids with various pharmacological effects, such as antiviral, antibacterial, antimalarial, antifungal, and cytotoxic activities. This study aimed to isolate, elucidate, and evaluate the cytotoxicity of limonoids from C. pentandrus stembarks. Isolation was carried out using various separation methods including extraction and column chromatography. Spectral data were analyzed by FT-IR, UV, DEPT 135°, ¹H, ¹³C-NMR, and HRTOF-MS and compared with those previously reported to determine the chemical structure.The obtained limonoids were lasiocarpine (1), lasiocarpine B (2), pentandricine (3), and 16β-hydroxydysobinin (4); all of which were …

Degradation Of Chlorpyrifos Residue On Tropical Inceptisol Soil With Indigenous Bacteria Treatment, Asep Kurnia, Hidayatuz Zu`Amah, Baiq Nunung Sulastri, Ria Fauriah Makmur

Makara Journal of Science

The use of soil microbes is one of the most effective methods for degrading pesticide residue in soil. Microbes can accelerate the degradation of persistent pollutants including chlorpyrifos in soil. This research aims to find soil bacteria with the greatest potential to degrade chlorpyrifos residue in tropical inceptisol soil and determine its degradation index (DI). The soil used in this experiment was collected from agricultural fields in the districts of Demak, Central Java Province, Indonesia. The experiment was performed using factorial randomize complete block design composed of two factors and four replications. The first factors were made up of two …

Cow-Calf Retained Ownership Analysis And Price Forecasting Using An Online Decision-Support Tool, Dillon M. Feuz

All Current Publications

Cow-calf producers frequently must make the decision to either sell their calves at weaning or soon thereafter or retain ownership of the calves through a background/growing phase. While the price they might receive at weaning may be known, there is great uncertainty about what cattle prices might be at the end of the retained ownership program. This fact sheet explains a method for reducing some of the uncertainty with cattle prices when the sale will occur months in the future and introduces a web-based decision-support tool that can help cattle producers quickly analyze the potential returns and risks from various …

Hypoxia Inducible Factor 2Α Promotes Tolerogenic Macrophage Development During Cardiac Transplantation Through Transcriptional Regulation Of Colony Stimulating Factor 1 Receptor, Matthew Deberge, Samantha Schroth, Fanfan Du, Xin Yi Yeap, Jiao-Jing Wang, Mohammed Javeed Ansari, Edward B Thorp

Faculty, Staff and Student Publications

Solid organ transplantation mobilizes myeloid cells, including monocytes and macrophages, which are central protagonists of allograft rejection. However, myeloid cells can also be functionally reprogrammed by perioperative costimulatory blockade to promote a state of transplantation tolerance. Transplantation tolerance holds promise to reduce complications from chronic immunosuppression and promote long-term survival in transplant recipients. We sought to identify different mediators of transplantation tolerance by performing single-cell RNA sequencing of acute rejecting or tolerized cardiac allografts. This led to the unbiased identification of the transcription factor, hypoxia inducible factor (HIF)-2α, in a subset of tolerogenic monocytes. Using flow cytometric analyses and mice …

Car T-Cell Expansion: Harmful Or Helpful?, Anath C Lionel, Sattva S Neelapu

Faculty, Staff and Student Publications

No abstract provided.

A Spatiotemporal Map Of Co-Receptor Signaling Networks Underlying B Cell Activation, Katherine J Susa, Gary A Bradshaw, Robyn J Eisert, Charlotte M Schilling, Marian Kalocsay, Andrew C Kruse

Faculty, Staff and Student Publications

The B cell receptor (BCR) signals together with a multi-component co-receptor complex to initiate B cell activation in response to antigen binding. Here, we take advantage of peroxidase-catalyzed proximity labeling combined with quantitative mass spectrometry to track co-receptor signaling dynamics in Raji cells from 10 s to 2 h after BCR stimulation. This approach enables tracking of 2,814 proximity-labeled proteins and 1,394 phosphosites and provides an unbiased and quantitative molecular map of proteins recruited to the vicinity of CD19, the signaling subunit of the co-receptor complex. We detail the recruitment kinetics of signaling effectors to CD19 and identify previously uncharacterized …

Cracd Loss Induces Neuroendocrine Cell Plasticity Of Lung Adenocarcinoma, Bongjun Kim, Shengzhe Zhang, Yuanjian Huang, Kyung-Pil Ko, Youn-Sang Jung, Gengyi Zou, Sohee Jun, Kee-Beom Kim, Kwon-Sik Park, Jae-Il Park

Faculty, Staff and Student Publications

Tumor cell plasticity contributes to intratumoral heterogeneity and therapy resistance. Through cell plasticity, some lung adenocarcinoma (LUAD) cells transform into neuroendocrine (NE) tumor cells. However, the mechanisms of NE cell plasticity remain unclear. CRACD (capping protein inhibiting regulator of actin dynamics), a capping protein inhibitor, is frequently inactivated in cancers. CRACD knockout (KO) is sufficient to de-repress NE-related gene expression in the pulmonary epithelium and LUAD cells. In LUAD mouse models, Cracd KO increases intratumoral heterogeneity with NE gene expression. Single-cell transcriptomic analysis showed that Cracd KO-induced NE cell plasticity is associated with cell de-differentiation and stemness-related pathway activation. The …

Cross-Sectional Analysis Of The Association Between Household Food Insecurity And Mental Health Conditions In Children Aged 5–11 Years In Canada, Justin Thielman, Sarah Orr, Saranya Naraentheraraja, Daniel Harrington, Sarah Carsley

Faculty, Staff and Student Publications

BACKGROUND: Children living in food insecure households have poorer mental health outcomes compared with their food-secure peers; however, the relationship between the severity of food insecurity and diagnosed mental health conditions in young children remains unknown. This study examined the association between household food insecurity and reported diagnosed mental health conditions among children aged 5-11 years in Canada.

METHODS: This study included 16 216 children aged 5-11 years living in Canada, from the 2019 Canadian Health Survey on Children and Youth. We measured household food insecurity using the Household Food Security Survey Module. We measured diagnosed mental health conditions by …

Plum Curculio (Conotrachelus Nenuphar), Kate V. Richardson, Marion Murray

All Current Publications

Plum curculio is a brown weevil (beetle with a snout) native to eastern North America, where it is a major pest of pome and stone fruits. It was detected in Box Elder County, Utah, in the early 1980s, and this population remains the only known infestation in western North America, where it is occasionally found in residential and wild fruit trees. Plum curculio is a quarantine pest in western North America (U.S. and Canada), and fruit grown in infested counties is restricted from being exported. Thus, it is a threat to Utah’s fruit industry and requires ongoing monitoring and management …