Life Sciences Commons^™

Performance Of Grazing Bull Calves Supplemented With Increasing Levels Of Ruminally Undegradable Portein, J R. Kawas, O Osmin, J Hernandez, R Leal, F Garza, J L. Danelon

IGC Proceedings (1977-2023)

The objective of this study was to determine the effect of increasing levels of ruminally undegradable protein (ECP) on the performance of bull calves grazing Coastcross-1 Bermudagrass during the dry season. One hundred and twelve Zebu-Brown Swiss bull calves weighing an average of 265 kg, were randomly assigned to four treatment groups in a completely random design. Calves received a self-feeding supplement (1 kg) with 40% crude protein (CP) and 31, 38, 44, or 52% ECP. During a 96-day preliminary phase without supplementation, weight gains of the four treatment groups ranged from 0.357 to 0.394 kg/day. A linear (P<0.0 I) response of calf weight gain was observed with an increase in ECP of the supplement (0.668, 0.672, 0.823 and 0.893 kg/day, respectively). Plasma urea concentration was reduced (P<0.05) from 17.8 to 9.85 mg/di with the highest level ofECP, which may reflect a reduced supply of NH -N for ruminal bacteria.

Relationships Between Rumen Degradability And Cell Wall- Binding Ferulic Acid Ethers In Various Forage Crops, M Goto

IGC Proceedings (1977-2023)

Ether-linked ferulic acid was determined in whole straw, plant parts and specific tissue cell walls of five barley (Hordium vulgare L.) cultivars and in sorghum stalks (Sorghum bicolor Moench x Sorghum sudanense Stapf) with five different growth and maturation stages and was related to their dry matter degradability (DMD) and extent of alkali-labile substitution at 0-5 arabinose residues previously determined.

The Energetic Utilization Of Forages In Fresh And Dried Condition By Ruminants, W Jentsch

IGC Proceedings (1977-2023)

The influence of dehydration of forages on digestibility and energy utilization was measured by indirect calorimetry in respiration chambers with adult wether sheep as a model for ruminants. Comparisons were carried out between fresh and carefully dried forages (experiment 1) and between fresh and not only carefully but also severely dried forages (experiment 2). Careful drying caused no change, but severe drying decreased energy and especially crude protein digestibility. There were no differences in utilization of metabolizable energy between fresh and carefully or severely dried forages.

Effect Of Variation In The Quality Of Forage Maize Silage In Ireland On Forage Intake And On Milk Production Of Dairy Cows, J J. Fitzgeald

IGC Proceedings (1977-2023)

In three experiments maize silages which varied in quality, i.e. starch content and dry matter digestibility (DMD) replaced a high proportion (60-67%) of grass silage in the diet of cows in early or mid-lactation. In each experiment the forages were supplemented with concentrates (4 or 6 kg/cow/day). Replacing high quality grass silages (DMD 750 g/kg) with high quality maize silages (starch 219 or 355 g/kg DM, DMD 694 or 754 g/kg) significantly increased forage intake, milk yield, milk protein concentration and the yield of fat and protein (Experiments 1 and 3). Replacing grass silage with a low starch maize silage …

Minerals In Forages Of Southern Italy Relate To Climate And Harvest Time Effects, G Convertini, D Ferri

IGC Proceedings (1977-2023)

Three cultivars of Alfalfa (Ascolana, Bresaola L 202 and Canè) in the Apulian “Tavoliere” (southern Italy) environment were studied. The effects of three water levels (watering of 1000 m3 ha-1 after each cut; 500 m3 ha-1 as above and another water supply with the same volume when the plant reached wilting conditions; with watering of 300 m3 ha-1 every 8 days to assure continuous water availability) and two cutting times (flower bud and 50% of flowering) were investigated. The cultivars showed a different mineral composition only for quantities not for qualities. The mineral composition of three cultivars were similarly influenced …

Foliar Supplementation Of Minerals To Prevent Calcium And Magnesium Deficiencies In Dairy Cows, G F. Wilson, W J. Courtney

IGC Proceedings (1977-2023)

The objectives of this study were to examine the possibility of reducing the incidence of calcium and magnesium deficiencies in cows by feeding anionic salts and by manipulating the mineral concentrations in pastures with foliar sprays during late pregnancy. Cows provided with a mineral supplement of magnesium chloride and ammonium sulphate before calving produced more milk solids and experienced less health problems. The foliar application of anionic salts, with and without sodium chloride, altered the cation balance in pastures grown on 6 different soils.

Genetic Variation In Perennial Ryegrass For Volatile Fatty Acid Production In Rumen Fluid, H.J P. Marvin, E N. Van Loo, O Dolstra, C.H A. Snijders, D Reheul, J W. Cone

IGC Proceedings (1977-2023)

In perennial ryegrass genetic variation was shown not only for in vitro organic matter degradation (OMD) in rumen fluid and neutral detergent fibre content (NDF) but also for production of volatile fatty acids (VFAs) upon in vitro incubation in rumen fluid. The correlations among concentrations of VFAs in the incubation fluid were high. OMD and NDF, on the other hand, were poorly correlated to the VFA concentrations.

Characterization Of Legume And Grass Residues Following In Vitro And In Sacco Ruminal Digestion, D.J R. Cherney, J H. Cherney, A H. Davidson

IGC Proceedings (1977-2023)

Objectives were to characterize the influence of forage species and maturity on dry matter degradation. Averaged across maturities, alfalfa and red clover were lower in NDF content (39 and 30% NDF, respectively) than reed canarygrass and timothy (59 and 54% NDF, respectively). The NDF of the DM residues in grass remained relatively unchanged over time, while NDF of the DM residue in legume increased rapidly for 9 h before becoming relatively constant. Alfalfa incubated in vitro (42% NDF) compared to reed canarygrass (58% NDF) contained more ND soluble material (39% vs. 25%), and more pepsin soluble material (22 vs. 18%). …

Potassium Accumulation In Perennial Cool-Season Grass Forage, J H. Cherney, D.J R. Cherney

IGC Proceedings (1977-2023)

Perennial grasses are well adapted to the northern U.S.A. and Canada, but the potassium (K) content of grass forage is a major concern in regard to dairy cattle nutrition. Our goal was to identify factors influencing potassium content of grasses, so that a grass management strategy for controlling potassium content could be formulated. Separate experiments were conducted with several perennial coolseason grass species and varieties. Nitrogen fertilization and harvest management also were evaluated. Aside from the obvious positive effect on K concentration caused by commercial K fertilizer or by animal manure application, K concentration was influenced by grass species, grass …

Influence Of Different Cutting Frequencies And Fertilization On Grass Silage Quality, U Wyss, R Vogel

IGC Proceedings (1977-2023)

The influence of different cutting frequencies and fertilization on dry matter yield, botanical composition, nutrient concentration and fermentation quality of grass silage was investigated. Altogether four treatments were compared; two 5-cut-treatnents with two different fertilizer levels, one 3-cut-treatment with fertilizer and one 2-cuttreatment without fertilizer. In the 5-cut-treatments the double fertilizer amount produced a 10% higher dry matter yield and also a higher proportion of grasses. The fermentation quality of these silages was mostly good, especially in autumn when the high nitrate content improved the quality. The 3- cut and 2-cut-variants showed a higher grass proportion, a higher crude fibre …

Seasonal Changes In Forage Quality Of C3 Grasses On Sagebrush Grassland, H F. Mayland, G E. Shewmaker

IGC Proceedings (1977-2023)

Annual and perennial grasses growing in the semiarid, west-central North American continent mature by early to midsummer. Forage mineral concentrations in these C-3 grasses decrease curvilinearly as the season advances from day of year (DOY) 75 to 300. Total digestible dry matter (TDDM) and digestible cell wall (DCW) decrease linearly with DOY while neutral detergent fiber (NDF) increases curvilinearly. By midsummer; energy, N, P, and Zn usually become deficient for ruminant nutrition.

Nitrogen Fertilizer Effects Upon Silage Composition And Quality Of Lolium Multiflorum, L., E M. Viviani Rossi, L M. Gutierrez, E Moreno, A Mazzanti

IGC Proceedings (1977-2023)

The objective of this study was to determine the ensiling properties of ryegrass (Lolium multiflorum L) fertilized with different doses of nitrogen. A pure ryegrass pasture was sown in March 1994 and six N doses (0, 50, 100, 150, 200 and 250 kg N.ha-1) were applied in August 1994. The lots were harvested 60 days after fertilization. The harvested material was chopped and stored for about 60 days in 5 l Polyvinyl chloride (PVC) laboratory silos, and replicated 3 times. Values of DM, IVDMD, TN, NDF, WSC, pH, NH3-N and Sol-N were determined. A completely randomized design was applied, …

Protein Degradability Effect And Supplementation Level On Pasture Utilization, A.V M. Franco, P De Andrade, G L. Franco

IGC Proceedings (1977-2023)

The objective was to determine the effect of protein degradability and supplement level on forage degradation. Steers fistulated were utilizated in brachiaria (Brachiaria brizantha) pasture. A randomized block design was used with a control treatment and supplements according to a factorial scheme combining low, medium and high protein degradability with supplementation levels: low, medium and high analysed as principal treatments and incubation time as splitplot by SAS. Nylon bags were incubated at 4; 8; 12; 24; 48; 72; 96 and 120 hours to determine DM, CP and NDF degradation. There was no effect (P>0.05) of protein degradability …

Breeding To Improve Yield And Quality In Napiergrass, Y K. Cheng, C S. Chen

IGC Proceedings (1977-2023)

The breeding program has been conducted to improve the production and quality of napiergrass by crossing with related species or intraspecies. CV. TLG2 with high yields and water soluble carbohydrates have been selected and released. It produced 20% more forage yields and water soluble carbohydrates than cultivar, CV. A146 and persisted well at least for five years. Good silage was obtained when the CV. TLG2 was harvested at the right time. Milking ability is improved by use of silage and/or green chopping whole year round.

Use Of Nir To Estimate Nitrogen Degradability Of Forage, R Agneeseens, P Dardenne, Ph Lecomte

IGC Proceedings (1977-2023)

Near infra red spectrometry (NIRS) is commonly used to characterise animal feeds at the level of their intrinsic composition (dry matter, protein, fat, fiber) as well of their digestibility (Dardenne et al.,1991). The use of new systems in the evaluation of the protein value has induced the necessity to determine parameters such as the theoretical ruminal degradability of nitrogen (DT).

The definition of this criterion necessitates the implementation of the nylon bag technique which is time consuming and costly and is reserved to reference laboratories. An alternative to this technique, based on an enzymatic hydrolysis of the proteinic content, has …

Energetic Feed Value Of Grassland Products In Ruminants, A Chudy

IGC Proceedings (1977-2023)

A method for calculating the energetic feed value, based on the digestible nutrients and on the relations of the utilisation of the energy of the nutrients (carbohydrates = 100) for ATP syntheses is described. This relative scale is a nominal feed value (NFV). It characterises the ATP potential of the feedstuffs and is therefore equivalent to the efficiency of utilisation of the feed energy in the catabolic metabolism (non-thermal maintenance, muscular work (movement)) and energy costs for syntheses as well as the productive performance as energy retention in animal products. Therefore this scale secures a precise precognition of the animal …

Field Evaluation Of Female- And Male-Targeted Traps For Ceratitis Capitata (Diptera: Tephritidae), Elliot T. Howse, Rieks Van Klinken, Nicholas J. Beeton, Helen Spafford, Kim P. James, Matthew P. Hill

Horticulture Research Articles

Mediterranean fruit fly (Medfly) Ceratitis capitata (Wiedemann) (Diptera: Tephritidae) is a globally significant economic pest for which lure based trapping can be used to monitor established populations and for surveillance. Either female- or male-targeted traps can be used; however, recommendations on which to apply are inconsistent and many programs rely on male-targeted traps. Here, we compare the performance of male-targeted traps (Lynfield Trap with Trimedlure) and female-targeted traps (Biotrap Globe trap with the 3-component lure—TMA Plus) in apple orchards in south-west Western Australia over 2 years (September 2019 to September 2021). Male-targeted traps caught more Medflies overall than female-targeted traps, …

Climate, Food, And Humans Predict Communities Of Mammals In The United States, Roland Kays, Matthew H. Snider, Andrew M. Wilson

Environmental Studies Faculty Publications

The assembly of species into communities and ecoregions is the result of interacting factors that affect plant and animal distribution and abundance at biogeographic scales. Here, we empirically derive ecoregions for mammals to test whether human disturbance has become more important than climate and habitat resources in structuring communities.

Exploring The Nexus Of Food Citizenship And Local Food Consumption In America's Rural-Urban Blend: Insights From Will County (Illinois), Jerry H. Kavouras, Marie Asma Ben-Othmen

Biology Department Faculty Articles

This paper uses Will County in Illinois as a case study to examine the relationship between food citizenship and local food consumption in what we term to be the rural-urban blend of America. Food citizenship is participating in food-related actions promoting democratic, socially and economically equitable, and environmentally sustainable food systems. On the other hand, local food consumption is choosing and preferring to buy and eat local food over other conventional forms of food. The study used data analysis from a survey of 342 residents in Will County, Illinois, to test a conceptual model that considers various socio-psychological traits that …

Comparison Of Sheep And Goats To The Acute Toxic Effects Of Foothill Death Camas, Kevin D. Welch, Stephen T. Lee, Clint A. Stonecipher, Dale R. Gardner, Daniel Cook

Poisonous Plant Research (PPR)

Death camas (Zigadenus spp) is a perennial forb found throughout the western United States, which is known to kill both sheep and cattle. In a previous study, goats appeared to be somewhat resistant to the adverse effects of death camas. Therefore, the objective of this study was to directly compare the susceptibility of goats and sheep to the acute toxic effects of death camas. Sheep and goats were dosed at 0.5, 1.0, 2.0, 4.0, and 6.0 g death camas per kg BW. The data presented in this manuscript suggest that goats are more susceptible to death camas than sheep. …

The Influence Of Childhood Trauma On Dna Methylation Patterns And Sex-Specific Cortisol Stress Reactivity: A Secondary Analysis, Isabella F. Correia

USF Tampa Graduate Theses and Dissertations

Childhood trauma has been shown to significantly impact physical and mental health outcomes, leading to conditions such as heart disease, diabetes, depression, and difficulties in decision-making and maintaining relationships. Exposure to such trauma has the potential to dysregulate an individual’s stress response system, resulting in abnormal cortisol levels. Many studies suggest a lasting impact of early-life stress on DNA methylation patterns and, separately, cortisol stress reactivity. In addition, there are known sex differences in exposure to childhood trauma and cortisol reactivity; yet studies to date have not investigated whether there is an association to DNA methylation. This study utilized a …

Roles Of Mitochondrial Chchd2 And Gpcr-Associated Β-Arrestin2 In The Pathogenesis Of Lewy Body Disorders, Teresa R. Kee

USF Tampa Graduate Theses and Dissertations

Coiled-coil-helix-coiled-coil-helix domain containing 2 (CHCHD2) is a mitochondrial protein that plays important roles in cristae structure, oxidative phosphorylation, and apoptosis. Multiple mutations in CHCHD2 have been associated with Lewy body disorders (LBDs), such as Parkinson’s disease (PD) and dementia with Lewy bodies (DLB), with the CHCHD2T61I mutation being the most widely studied. We generated the first transgenic mouse models expressing human CHCHD2WT and the PD-linked CHCHD2T61I mutation driven by the mPrP promoter. CHCHD2T61I Tg, but not CHCHD2WT Tg mice exhibit perinuclear mitochondrial aggregates, neuroinflammation, and have impaired long-term synaptic plasticity associated with synaptic dysfunction. Dopaminergic neurodegeneration, a hallmark of PD, …

The Role Of L-Fucose In Dendritic Cell Antigen Presentation And Polarization, Chase J. Burton

USF Tampa Graduate Theses and Dissertations

Since the FDA approval of IL-2 in cancer treatment in the 1980s, immunotherapy has emerged as the standard of care for several tumor types (e.g., melanoma, lung, bladder, colorectal). However, approximately 70 percent of cancer patients, independent of prior treatment or staging, administered immunotherapy will not achieve complete responses and will exhibit disease progression. Several factors have been identified to contribute to treatment failure, including the accumulation of intratumoral immunosuppressive cell populations, insufficient tumor-infiltrating anti-tumor immune populations, or the occurrence of immunoediting on tumor cells. Current strategies to circumvent immunosuppression either lack durable response to new immunosuppressive mechanisms the tumor …

Characterization And Reprogramming Of T-Cell Metabolic Dysfunction In Chronic Lymphocytic Leukemia For Improved Adoptive Cell Therapy, Wael B. Gamal

USF Tampa Graduate Theses and Dissertations

Chronic lymphocytic leukemia (CLL) is a predominant B-cell malignancy impacting elderly populations in Western societies. Targeted therapies have revolutionized the CLL treatment landscape. Still, patients often require long-term therapy and are subject to drug-induced resistance and severe toxicities, for which CLL is still considered an incurable disease. T-cell-based immunotherapy, particularly chimeric antigen receptor (CAR) T cells, has shown remarkable success, leading to various FDA approvals in hematological malignancies. Nonetheless, CAR T-cell therapy success rate is limited in CLL because of the dysfunctional nature of T cells. CLL T cells exhibit skewing toward immunosuppressive and terminally differentiated phenotypes, leading to hindrances …

The Significant Associations Between Epigenetic Clocks And Bladder Cancer Risks, Yang Deng, Chia-Wen Tsai, Wen-Shin Chang, Yifan Xu, Maosheng Huang, Jian Gu

Faculty, Staff and Student Publications

Bladder cancer is an age-related disease, with over three-quarters of cases occurring in individuals aged 65 years and older. Accelerated biological aging has been linked to elevated cancer risks. Epigenetic clocks serve as excellent predictors of biological age, yet it remains unclear whether they are associated with bladder cancer risk. In this large case-control study, we assessed the associations between four well-established epigenetic clocks-HannumAge, HorvathAge, GrimAge, and PhenoAge-and bladder cancer risk. Utilizing single nucleotide polymorphisms (SNPs), which were identified in a genome-wide association study (GWAS), linked to these clocks as instruments, we constructed a weighted genetic risk score (GRS) for …

Developing A Fit-For-Purpose Composite Symptom Score As A Symptom Burden Endpoint For Clinical Trials In Patients With Malignant Pleural Mesothelioma, Charles S Cleeland, Karen N Keating, Brian Cuffel, Cem Elbi, Jonathan M Siegel, Tara Symonds, Amylou C Dueck, Andrew Bottomley, Xin Shelley Wang, Loretta A Williams, Tito R Mendoza

Faculty, Staff and Student Publications

We developed a composite symptom score (CSS) representing disease-related symptom burden over time in patients with malignant pleural mesothelioma (MPM). Longitudinal data were collected from an open-label Phase IIB study in which 239 patients completed the validated MD Anderson Symptom Inventory for MPM (MDASI-MPM). A blinded, independent review committee of external patient-reported outcomes experts advised on MDASI-MPM symptoms to include in the CSS. Through iterative analyses of potential symptom-item combinations, 5 MPM symptoms (pain, fatigue, shortness of breath, muscle weakness, coughing) were selected. The CSS correlated strongly with the full MDASI-MPM symptom set (0.92-0.94) and the Lung Cancer Symptom Scale-Mesothelioma …

Cgmp Compliant One-Step, One-Pot Automated [18f]Fbntp Production For Clinical Imaging Of Mitochondrial Activity, Mai Lin, Cong-Dat Pham, Robert T Ta, H Charles Manning

Faculty, Staff and Student Publications

Background

4-[18F]fluorobenzyl-triphenylphosphonium ([18F]FBnTP) is a lipophilic cation PET tracer. The cellular uptake of [18F]FBnTP is correlated with oxidative phosphorylation by mitochondria, which has been associated with multiple critical diseases. To date, [18F]FBnTP has been successfully applied for imaging myocardial perfusion, assessment of severity of coronary artery stenosis, delineation of the ischemic area after transient coronary occlusion, and detection/quantification of apoptosis in various animal models. Recent preclinical and clinical studies have also expanded the possibilities of using [18F]FBnTP in oncological diagnosis and therapeutic monitoring. However, [18F]FBnTP is typically prepared through a tediously lengthy four-step, three-pot reaction and required multiple synthesizer modules; …

Dietary Saturated Fatty Acids Modulate Host Innate Immunity, Amy Lorraine Seufert

Dissertations and Theses

Nutrition, cellular metabolism, and inflammatory regulation of the immune system are interconnected, and advancing our understanding of this will greatly improve treatment of metabolic and inflammatory disease. Specifically, dietary saturated fats are becoming increasingly appreciated for their ability to modify innate immune cell function in mammals following absorption into the blood and lymphatic circulation systems. The work herein focuses on the capacity for lipids in the diet to modulate innate immune memory. My thesis first broadly outlines fundamental background, including: the evolution of human nutrition, the discovery of innate immune memory, and key topics that reoccur throughout the text surrounding …

Stakeholder Involvement And Systematic Review Of Elements That Determine A Successful Return To Work For Injured Workers: A Scoping Review, Sundip Kaur Khosa, Wilma Jelley Professor

Internet Journal of Allied Health Sciences and Practice

Introduction: A healthy and sustainable return to work (RTW) for a worker after injury or illness has benefits for the worker, their family, the employer, and society.¹ For this research we used the following definition of RTW success; return to positive and meaningful work in a position or occupation not necessarily including all the aspects of the worker’s pre-injury or pre-illness functions or tasks. Purpose: The question behind this survey-based research was, What elements are perceived as essential, to ensure return to employment of injured or ill workers, by stakeholders with experience in the RTW process? Methods: A 32 …

Rapid Changes In Cerebrovascular Compliance During Vasovagal Syncope., Leena N Shoemaker, Aleena Sajid, Ronald Schondorf, Kevin Shoemaker

Kinesiology Publications

PURPOSE: The compensatory mechanisms supporting cerebral perfusion throughout head-up tilt (HUT) in patients with vasovagal syncope (VVS) remain unclear. We tested the hypothesis that increased cerebrovascular compliance (Ci) and decreased cerebrovascular resistance (CVR) support cerebral blood velocity (CBV) during pre-syncope in VVS.

METHODS: Finger arterial blood pressure (ABP) and right middle cerebral artery blood velocity (CBV) were recorded in 15 individuals diagnosed with VVS (n = 11 female, mean age: 40 ± 16 years, mean body mass index: 24.9 ± 4.0 kg/m

RESULTS: Diastolic ABP, systolic ABP, mean ABP (72 ± 11 to 51 ± 12 mmHg), and CVR decreased …