Life Sciences Commons^™

Targeting Prmt3 Impairs Methylation And Oligomerization Of Hsp60 To Boost Anti-Tumor Immunity By Activating Cgas/Sting Signaling, Yunxing Shi, Zongfeng Wu, Shaoru Liu, Dinglan Zuo, Yi Niu, Liang Qiao, Jiliang Qiu, Yunfei Yuan, Guocan Wang, Binkui Li

Faculty, Staff and Student Publications

Immune checkpoint blockade (ICB) has emerged as a promising therapeutic option for hepatocellular carcinoma (HCC), but resistance to ICB occurs and patient responses vary. Here, we uncover protein arginine methyltransferase 3 (PRMT3) as a driver for immunotherapy resistance in HCC. We show that PRMT3 expression is induced by ICB-activated T cells via an interferon-gamma (IFNγ)-STAT1 signaling pathway, and higher PRMT3 expression levels correlate with reduced numbers of tumor-infiltrating CD8+ T cells and poorer response to ICB. Genetic depletion or pharmacological inhibition of PRMT3 elicits an influx of T cells into tumors and reduces tumor size in HCC mouse models. Mechanistically, …

Transcriptomic And Proteomic Differences In Btk-Wt And Btk-Mutated Cll And Their Changes During Therapy With Pirtobrutinib, Burcu Aslan, Ganiraju Manyam, Lakesla R Iles, Shady I Tantawy, Sai Prasad Desikan, Varsha Gandhi

Faculty, Staff and Student Publications

Covalent Bruton tyrosine kinase inhibitors (cBTKis), which bind to the BTK C481 residue, are now primary therapeutics for chronic lymphocytic leukemia (CLL). Alterations at C481, primarily C481S, prevent cBTKi binding and lead to the emergence of resistant clones. Pirtobrutinib is a noncovalent BTKi that binds to both wild-type (WT) and C481S-mutated BTK and has shown efficacy in BTK-WT and -mutated CLL patient groups. To compare baseline clinical, transcriptomic, and proteomic characteristics and their changes during treatment in these 2 groups, we used 67 longitudinal peripheral blood samples obtained during the first 3 cycles of treatment with pirtobrutinib from 18 patients …

The Influence Of Hip Position On Neck Kinematics And Muscle Activity In Healthy And Non-Specific Neck Pain Subjects, Enrique Lopez, Everett Lohman, Noha Daher, Mansoor Alameri, Robert Dudley

International Journal of Exercise Science

International Journal of Exercise Science 17(1): 1280-1293, 2024. Neck pain is a widespread problem in society with many variables influencing its cause. The angle of the hip may influence the kinematics of the neck in addition to the myoelectrical activation of the surrounding musculature that contributes to the development of neck pain. The purpose of this study was to investigate the changes in spinal inclination angles and muscle activity in the neck using a 10 deg wedge in a forward slope, neutral and rear slope seated position. Forty subjects were recruited for this study. Each subject sat at a workstation …

Genotypic And Phenotypic Characterisation Of Asymptomatic Bacteriuria (Abu) Isolates Displaying Bacterial Interference Against Multi-Drug Resistant Uropathogenic E. Coli, Ciara Kenneally, Craig P. Murphy, Roy D. Sleator, Eamonn P. Culligan

Department of Biological Sciences Publications

Escherichia coli can colonise the urogenital tract of individuals without causing symptoms of infection, in a condition referred to as asymptomatic bacteriuria (ABU). ABU isolates can protect the host against symptomatic urinary tract infections (UTIs) by bacterial interference against uropathogenic E. coli (UPEC). The aim of this study was to investigate the genotypic and phenotypic characteristics of five ABU isolates from midstream urine samples of adults. Comparative genomic and phenotypic analysis was conducted including an antibiotic resistance profile, pangenome analysis, and a putative virulence profile. Based on the genome analysis, the isolates consisted of one from phylogroup A, three from …

Pseudomonas Aeruginosa And Tobramycin Effects On Aspergillus Fumigatus Infection In Cystic Fibrosis Airway, Tuan Anh Nguyen

All ETDs from UAB

Aspergillus fumigatus (Af) is one of the most common molds in the airways of people with Cystic Fibrosis (pwCF). The infection of Af is often preceded by Pseudomonas aeruginosa (PsA) infection and its correlated treatment. PsA is considered a highly prevalent bacteria in the CF lung. PwCF suffers from complex and sophisticated coinfections within the airway that negatively impact the health outcomes of patients. Despite this, why fungal and bacterial coinfection occurs in pwCF is still being determined. However, abnormal mucus production and its characteristics are suspected of creating an environment that promotes Af germination and disease progression. This study …

Improving Molecular Diagnosis Success Rates And Prognosis Prediction For Patients With Rare Diseases, Tarun Karthik Kumar Mamidi

All ETDs from UAB

Rare diseases are medical conditions that affect a small number of people compared to the general population. There are more than 10,000 estimated rare diseases with 80% being genetic in origin, which although individually have a relatively low prevalence, collectively impact the lives of hundreds of millions of people worldwide. The diagnosis process is usually difficult and slow, but an accurate and early diagnosis can be crucial for disease treatment and management. This dissertation focuses on some of the important problems in this area by investigating the application of advanced machine learning methods to enhance the accuracy, precision, and efficiency …

Individualized Cellular Response To Influenza-A Infection, Abel Lopez

All ETDs from UAB

Influenza A Virus (IAV) presents a significant threat to global health, causing millions of severe cases and substantial mortality annually. This study uses single-cell RNA sequencing to investigate the individualized cellular response to IAV infection in human bronchial epithelial cells (HBECs). We employed air-liquid interface (ALI) cultures to model the respiratory epithelium and differentiated HBECs into basal, ciliated, secretory (goblet/club), and rare cell types (Ionocytes). Following IAV infection, we analyzed transcriptional changes across these cell types, focusing on differential gene expression and pathway activation. Our results highlight the variability in IAV infection and the distinct cellular responses. Ciliated cells, the …

Testicular Phenotypes Of Rdhe Dko Mice, Aja Slay

All ETDs from UAB

The bioactive form of vitamin A, all-trans-retinoic acid (RA), plays a critical role in cell differentiation, metabolism, and cellular proliferation by regulating gene transcription. RA signaling is critical during embryogenesis to ensure proper development and during adulthood for tissue maintenance. Disruption of the RA signaling pathway induced by vitamin A deficiency and genetic defects in vitamin A metabolizing enzymes results in embryonic malformations. Previous studies implicate the short-chain dehydrogenase/reductase (SDR) family members as important in RA synthesis. Among the SDR family are retinol dehydrogenase epidermal 2 (RDHE2) and RDHE2-similar (RDHE2S)- collectively RDHE. To gain insight into how RDHE influences the …

Biology Department Publications: 2014, Sam Lindgren

Biology Department Bibliographies

This document is a noncomprehensive list of publications made by graduate students, post-doctoral researchers, and faculty members in the Biology Department in 2014. It indexes those publications available through major databases, and serves as an archival record of the Department’s robust scholarly contributions.

Biology Department Publications: 2017, Sam Lindgren

Biology Department Bibliographies

This document is a noncomprehensive list of publications made by graduate students, post-doctoral researchers, and faculty members in the Biology Department in 2017. It indexes those publications available through major databases, and serves as an archival record of the Department’s robust scholarly contributions.

Malat1 Mediates Tet2 Loss Of Function-Driven Oncogenic Inflammation Via Rna Shielding Of Nf- Kappa B P65, Nana Adjoa Ben-Crentsil

USF Tampa Graduate Theses and Dissertations

TET2 is a dioxygenase that regulates gene expression and hematopoiesis by oxidizing 5-methylcytosine residues on DNA to 5-hydroxymethylcytosine. TET2 mutations are common genetic events in hematological disorders. For example, TET2 is the most frequently mutated gene in Chronic Myelomonocytic Leukemia and occurs in about 33% of other myeloid malignancies. It is also the second commonest mutation in clonal hematopoiesis of indeterminate potential (CHIP), a common premalignant condition in the elderly. TET2 mutations arise in founding clones and are implicated in many non-hematological sequelae seen in CHIP like atherosclerotic disease, chronic liver disease, and severe microbial infections.

The development of TET2-deficient …

Biology Department Publications: 2009, Sam Lindgren

Biology Department Bibliographies

This document is a noncomprehensive list of publications made by graduate students, post-doctoral researchers, and faculty members in the Biology Department in 2009. It indexes those publications available through major databases, and serves as an archival record of the Department’s robust scholarly contributions.

Opportunities For Extension Educators To Support Caregivers Feeding Infants And Toddlers Through Nutrition Education, Elizabeth I. Kielb, Kameron J. Moding, Susan L. Johnson, Barbara A. Beaulieu, Blake A. Connolly, Stephanie J. Faroh, Laura L. Bellows

Journal of Extension

The 2020 Dietary Guidelines for Americans now include recommendations for infants and toddlers. Extension educators can use these expanded guidelines to provide feeding recommendations for caregivers of infants/toddlers. The purpose of this study was to explore 1) the infant and toddler feeding topics that Extension educators discuss with caregivers through formal curricula and informal conversations, 2) educators’ perceived barriers to teaching and discussing topics with caregivers, and 3) the optimal format of resources for educators and the communities they serve. Findings from this study confirmed a need among educators for infant/toddler feeding programs and resources offered through Extension.

Synergistic Inhibition Of Hiv-1 Regulatory Function Using A Dual Expression Shrna Plasmid, Christine Sanderson, William H. Jackson

Journal of the South Carolina Academy of Science

HIV infection of CD4⁺ T Helper lymphocytes results in a gradual loss of immune competence, which ultimately leads to development of the acquired immune deficiency syndrome (AIDS). HIV-1 expresses two regulatory proteins, Tat and Rev, which act to upregulate viral transcription and facilitate mRNA transport enhancing the rate of translation. Inhibition of Tat and Rev have been shown to block virus replication. Therefore, Tat and Rev represent critical key points in the virus replication cycle. This study investigated the potential of RNA interference (RNAi) to inhibit these proteins using short hairpin RNAs (shRNAs). For this, plasmids expressing shRNAs targeting …

09.09.2024 Orsp Connect, Liz Williamson

ORED Newsletter

New training course available: Sponsored Travel Guidance

OpenScholar Overview

Learn about Nature Masterclasses On-Demand Training

NSF Sessions on Phase 1 ISBIR/STTR Proposals

Biology Department Publications: 2005, Sam Lindgren

Biology Department Bibliographies

This document is a noncomprehensive list of publications made by graduate students, post-doctoral researchers, and faculty members in the Biology Department in 2005. It indexes those publications available through major databases, and serves as an archival record of the Department’s robust scholarly contributions.

Biology Department Publications: 2011, Sam Lindgren

Biology Department Bibliographies

This document is a noncomprehensive list of publications made by graduate students, post-doctoral researchers, and faculty members in the Biology Department in 2011. It indexes those publications available through major databases, and serves as an archival record of the Department’s robust scholarly contributions.

Biology Department Publications: 2007, Sam Lindgren

Biology Department Bibliographies

This document is a noncomprehensive list of publications made by graduate students, post-doctoral researchers, and faculty members in the Biology Department in 2007. It indexes those publications available through major databases, and serves as an archival record of the Department’s robust scholarly contributions.

Biology Department Publications: 2022, Sam Lindgren

Biology Department Bibliographies

This document is a noncomprehensive list of publications made by graduate students, post-doctoral researchers, and faculty members in the Biology Department in 2022. It indexes those publications available through major databases, and serves as an archival record of the Department’s robust scholarly contributions.

Biology Department Publications: 2018, Sam Lindgren

Biology Department Bibliographies

This document is a noncomprehensive list of publications made by graduate students, post-doctoral researchers, and faculty members in the Biology Department in 2018. It indexes those publications available through major databases, and serves as an archival record of the Department’s robust scholarly contributions.

Biology Department Publications: 2020, Sam Lindgren

Biology Department Bibliographies

This document is a noncomprehensive list of publications made by graduate students, post-doctoral researchers, and faculty members in the Biology Department in 2020. It indexes those publications available through major databases, and serves as an archival record of the Department’s robust scholarly contributions.

Biology Department Publications: 2006, Sam Lindgren

Biology Department Bibliographies

This document is a noncomprehensive list of publications made by graduate students, post-doctoral researchers, and faculty members in the Biology Department in 2006. It indexes those publications available through major databases, and serves as an archival record of the Department’s robust scholarly contributions.

Biology Department Publications: 2016, Sam Lindgren

Biology Department Bibliographies

This document is a noncomprehensive list of publications made by graduate students, post-doctoral researchers, and faculty members in the Biology Department in 2016. It indexes those publications available through major databases, and serves as an archival record of the Department’s robust scholarly contributions.

Biology Department Publications: 2019, Sam Lindgren

Biology Department Bibliographies

This document is a noncomprehensive list of publications made by graduate students, post-doctoral researchers, and faculty members in the Biology Department in 2019. It indexes those publications available through major databases, and serves as an archival record of the Department’s robust scholarly contributions.

Biology Department Publications: 2012, Sam Lindgren

Biology Department Bibliographies

This document is a noncomprehensive list of publications made by graduate students, post-doctoral researchers, and faculty members in the Biology Department in 2012. It indexes those publications available through major databases, and serves as an archival record of the Department’s robust scholarly contributions.

Biology Department Publications: 2015, Sam Lindgren

Biology Department Bibliographies

This document is a noncomprehensive list of publications made by graduate students, post-doctoral researchers, and faculty members in the Biology Department in 2015. It indexes those publications available through major databases, and serves as an archival record of the Department’s robust scholarly contributions.

Biology Department Publications: 2010, Sam Lindgren

Biology Department Bibliographies

This document is a noncomprehensive list of publications made by graduate students, post-doctoral researchers, and faculty members in the Biology Department in 2010. It indexes those publications available through major databases, and serves as an archival record of the Department’s robust scholarly contributions.

Biology Department Publications: 2013, Sam Lindgren

Biology Department Bibliographies

This document is a noncomprehensive list of publications made by graduate students, post-doctoral researchers, and faculty members in the Biology Department in 2013. It indexes those publications available through major databases, and serves as an archival record of the Department’s robust scholarly contributions.

Biology Department Publications: 2023, Sam Lindgren

Biology Department Bibliographies

This document is a non-comprehensive list of publications made by graduate students, post-doctoral researchers, and faculty members in the Biology Department in 2023. It indexes those publications available through PubMed and Scopus, and serves as an archival record of the Department’s robust scholarly contributions.

Biology Department Publications: 2008, Sam Lindgren

Biology Department Bibliographies

This document is a noncomprehensive list of publications made by graduate students, post-doctoral researchers, and faculty members in the Biology Department in 2008. It indexes those publications available through major databases, and serves as an archival record of the Department’s robust scholarly contributions.