Life Sciences Commons^™

Rapid Unleashing Macrophage Efferocytic Capacity By Transcriptional Pause Release, Turan Tufan

Arts & Sciences Graduate Student Theses and Dissertations

During development, inflammation or tissue injury, macrophages engulf multiple apoptotic cells via efferocytosis to maintain tissue homeostasis. The mechanism by which macrophages rapidly adapt their transcription for continuous corpse uptake is not well understood. Transcriptional pause/release is a conserved mechanism, in which RNA polymerase (Pol) II initiates transcription for 20-60 nucleotides, is paused for minutes to hours and is then released to the elongation phase. We show that macrophages quickly use Pol II pause/release upon encountering corpses to trigger a rapid transcriptional response. This mechanism is essential for continuous efferocytosis but does not affect other forms of phagocytosis. Genomic data …

The Role Of Group 2 Innate Lymphoid Cells In Restoring Lung Health And Chitinase Function After Epithelial Injury, Haerin Jung

Arts & Sciences Graduate Student Theses and Dissertations

Alveolar epithelial cells provide oxygen transport and act as a first line of defense against pathogens and environmental triggers that disturb tissue homeostasis. However, in the context of epithelial injury, fibrotic and inflammatory lung diseases develop where regenerative responses may become dysregulated or recurrently engaged. One cause of lung injury is infection with cytopathic respiratory viruses such as influenza and SARS-CoV-2 viruses, which induce lung inflammation, tissue damage, and epithelial cell injury and loss. Restoration of barrier integrity after lung injury is critical for re-establishing healthy lung function but the underlying mechanisms are unclear. In the steady state, lung epithelial …

The Role Of Group 2 Innate Lymphoid Cells In Restoring Lung Health And Chitinase Function After Epithelial Injury, Haerin Jung

Arts & Sciences Graduate Student Theses and Dissertations

Alveolar epithelial cells provide oxygen transport and act as a first line of defense against pathogens and environmental triggers that disturb tissue homeostasis. However, in the context of epithelial injury, fibrotic and inflammatory lung diseases develop where regenerative responses may become dysregulated or recurrently engaged. One cause of lung injury is infection with cytopathic respiratory viruses such as influenza and SARS-CoV-2 viruses, which induce lung inflammation, tissue damage, and epithelial cell injury and loss. Restoration of barrier integrity after lung injury is critical for re-establishing healthy lung function but the underlying mechanisms are unclear. In the steady state, lung epithelial …

Rapid Unleashing Macrophage Efferocytic Capacity By Transcriptional Pause Release, Turan Tufan

Arts & Sciences Graduate Student Theses and Dissertations

During development, inflammation or tissue injury, macrophages engulf multiple apoptotic cells via efferocytosis to maintain tissue homeostasis. The mechanism by which macrophages rapidly adapt their transcription for continuous corpse uptake is not well understood. Transcriptional pause/release is a conserved mechanism, in which RNA polymerase (Pol) II initiates transcription for 20-60 nucleotides, is paused for minutes to hours and is then released to the elongation phase. We show that macrophages quickly use Pol II pause/release upon encountering corpses to trigger a rapid transcriptional response. This mechanism is essential for continuous efferocytosis but does not affect other forms of phagocytosis. Genomic data …

Impact Of Cancer Therapy On Clonal Hematopoiesis Mutations And Subsequent Clinical Outcomes, Kevin T Nead, Taebeom Kim, Lijin Joo, Tina L Mcdowell, Justin W Wong, Elizabeth Brock, Ting Xu, Chad Tang, Chang-Lung Lee, Jun-Ichi Abe, Kelly L Bolton, Zhongxing Liao, Paul A Scheet, Steven H Lin

Faculty, Staff and Student Publications

Exposure to cancer therapies is associated with an increased risk of clonal hematopoiesis (CH). The objective of our study was to investigate the genesis and evolution of CH after cancer therapy. In this prospective study, we undertook error-corrected duplex DNA sequencing in blood samples collected before and at 2 time points after chemoradiation in patients with esophageal or lung cancer recruited from 2013 to 2018. We applied a customized workflow to identify the earliest changes in CH mutation count and clone size and determine their association with clinical outcomes. Our study included 29 patients (87 samples). Their median age was …

Fast, Variable Stiffness-Induced Braided Coiled Artificial Muscles, Xinghao Hu, Xiangyu Wang, Jian Wang, Guorong Zhang, Shaoli Fang, Ye Xiao, Ray H Baughman, Jianning Ding

Faculty, Staff and Student Publications

Biomimetic actuation technologies with high muscle strokes, cycle rates, and work capacities are necessary for robotic systems. We present a muscle type that operates based on changes in muscle stiffness caused by volume expansion. This muscle is created by coiling a mechanically strong braid, in which an elastomer hollow tube is adhesively attached inside. We show that the muscle reversibly contracts by 47.3% when driven by an oscillating input air pressure of 120 kilopascals at 10 Hz. It generates a maximum power density of 3.0 W/g and demonstrates a mechanical contractile efficiency of 74%. The muscle's low-pressure operation allowed for …

Rapid Hyperspectral Photothermal Mid-Infrared Spectroscopic Imaging From Sparse Data For Gynecologic Cancer Tissue Subtyping, Reza Reihanisaransari, Chalapathi Charan Gajjela, Xinyu Wu, Ragib Ishrak, Sara Corvigno, Jinsong Liu, David Mayerich, Sebastian Berisha, Rohith Reddy

Faculty, Staff and Student Publications

Ovarian cancer detection has traditionally relied on a multistep process that includes biopsy, tissue staining, and morphological analysis by experienced pathologists. While widely practiced, this conventional approach suffers from several drawbacks: it is qualitative, time-intensive, and heavily dependent on the quality of staining. Mid-infrared (MIR) hyperspectral photothermal imaging is a label-free, biochemically quantitative technology that, when combined with machine learning algorithms, can eliminate the need for staining and provide quantitative results comparable to traditional histology. However, this technology is slow. This work presents a novel approach to MIR photothermal imaging that enhances its speed by an order of magnitude. This …

Ct Strain Metrics Allow For Earlier Diagnosis Of Bronchiolitis Obliterans Syndrome After Hematopoietic Cell Transplant, Husham Sharifi, Christopher D Bertini, Mansour Alkhunaizi, Maria Hernandez, Zayan Musa, Ihsan Turk, Burton F Dickey, Guang-Shing Cheng, Gregory Yanik, Craig J Galban, Huawei Henry Guo, Myrna C B Godoy, Joseph M Reinhardt, Eric A Hoffman, Mario Castro, Gabriela Rondon, Amin M Alousi, Richard E Champlin, Elizabeth J Shpall, Ying Lu, Samuel Peterson, Keshav Datta, Mark R Nicolls, Joe Hsu, Ajay Sheshadri

Faculty, Staff and Student Publications

Bronchiolitis obliterans syndrome (BOS) after hematopoietic cell transplantation (HCT) is associated with substantial morbidity and mortality. Quantitative computed tomography (qCT) can help diagnose advanced BOS meeting National Institutes of Health (NIH) criteria (NIH-BOS) but has not been used to diagnose early, often asymptomatic BOS (early BOS), limiting the potential for early intervention and improved outcomes. Using pulmonary function tests (PFTs) to define NIH-BOS, early BOS, and mixed BOS (NIH-BOS with restrictive lung disease) in patients from 2 large cancer centers, we applied qCT to identify early BOS and distinguish between types of BOS. Patients with transient impairment or healthy lungs …

The Impact Of A Web-Based Prognostic Calculator On Prognostic Confidence In Outpatient Palliative Care, David Hui, John P Maxwell, Allison De La Rosa, Kristofer Jennings, Marieberta Vidal, Ahsan Azhar, Kimberson Tanco, Yvonne Heung, Marvin Delgado-Guay, Donna Zhukovsky, Joseph Arthur, Suresh Reddy, Sriram Yennu, Amy Ontai, Eduardo Bruera

Faculty, Staff and Student Publications

Purpose: Clinicians are often uncertain about their prognostic estimates, which may impede prognostic communication and clinical decision-making. We assessed the impact of a web-based prognostic calculator on physicians' prognostic confidence.

Methods: In this prospective study, palliative care physicians estimated the prognosis of patients with advanced cancer in an outpatient clinic using the temporal, surprise, and probabilistic approaches for 6 m, 3 m, 2 m, 1 m, 2 w, 1 w, and 3 d. They then reviewed information from www.predictsurvival.com , which calculated survival estimates from seven validated prognostic scores, including the Palliative Prognostic Score, Palliative Prognostic Index, and Palliative Performance …

Atr Inhibition Radiosensitizes Cells Through Augmented Dna Damage And G2 Cell Cycle Arrest Abrogation, Scott J Bright, Mandira Manandhar, David B Flint, Rishab Kolachina, Mariam Ben Kacem, Broderick X Turner, Conor H Mcfadden, Poliana C Marinello, Simona F Shaitelman, Gabriel O Sawakuchi

Faculty, Staff and Student Publications

Ataxia telangiectasia and Rad3-related protein (ATR) is a key DNA damage response protein that facilitates DNA damage repair and regulates cell cycle progression. As such, ATR is an important component of the cellular response to radiation, particularly in cancer cells, which show altered DNA damage response and aberrant cell cycle checkpoints. Therefore, ATR's pharmacological inhibition could be an effective radiosensitization strategy to improve radiotherapy. We assessed the ability of an ATR inhibitor, AZD6738, to sensitize cancer cell lines of various histologic types to photon and proton radiotherapy. We found that radiosensitization took place through persistent DNA damage and abrogated G2 …

Study Of A Lemon, Marcella Carson

PANDION: The Osprey Journal of Research and Ideas

No abstract provided.

Hopper Hues, Hope S. Sohn

PANDION: The Osprey Journal of Research and Ideas

Artist Statement

From the elusive to the invasive, the insect world is often underrepresented in art. I created this piece to illustrate the beauty and intricacy of Romalea microptera, an invasive and destructive grasshopper species. Despite its impact on the environment, the beauty of this organism is apparent. A cascade of different appearances marks its life span until it reaches its final form; an array of oranges, pinks, and yellows.

What causes the division in public opinion on insects? What traits cause the recoil? There is beauty in both the beetle and the grasshopper, not just the butterfly. Expanding our …

Latticed Stinkhorn, Emilee G. Seigworth

PANDION: The Osprey Journal of Research and Ideas

Artist Statement

Latticed Stinkhorn is a watercolor piece originally created for the special gallery of the 2023 Scientific Illustration course at the University of North Florida. It was inspired by my enduring fascination with the world of fungi, particularly specimens of the family Phallaceae. This watercolor piece offers a simple perspective of Clathrus ruber, a species commonly referred to as Latticed Stinkhorn. Like many members of the family, the reproductive fruiting body first surfaces encased in a cream-colored soft shell with a texture comparable to a leathery reptile egg. As the body ages, the initial shell disintegrates to reveal a …

The Ladybug, Alexandra M. Brisson

PANDION: The Osprey Journal of Research and Ideas

Artist Statement

The Ladybug is a piece inspired by my wide-eyed youth. As a child, I was in awe of the ladybug's (Coccinellidae) iconic red elytra adorned with their contrasting black spots. I was also told that encountering a ladybug meant good luck and to make a wish, which fueled my fascination. As I began studying biology, I discovered that they play a crucial ecological role as voracious predators of aphids and other plant pests. Their contribution to natural pest control underscores their importance in agriculture and ecosystem balance. Ladybugs are valued allies, not only in the eyes of sustainable …

The Moon Jelly, Adam Krogmann

PANDION: The Osprey Journal of Research and Ideas

Artist Statement

The Moon Jelly is a piece I created as a part of the Scientific Illustration course I participated in during the summer semester of 2023. This piece was inspired by the livestreams of the Monterey Bay Aquarium that film these moon jellies (Aurelia aurita) in their tank. The Moon Jelly is an ink drawing created as part of a collection focused on Monterey Bay Aquarium animals. Through this artwork I hoped to draw attention to smaller marine animals that may otherwise be overlooked even though they provide a vital role in the ecosystem. Moon jellyfish are important marine …

Hepatic Targets To Control Blood Glucose And Potentially Aid In The Treatment Of Diabetes, Rena A. Velissarios

PANDION: The Osprey Journal of Research and Ideas

Maintaining glucose homeostasis is extremely important to remain in good health and prevent disease. This homeostasis is achieved via the regulation of hepatic metabolic pathways like gluconeogenesis and glycogenesis by insulin, which is expressed during high blood glucose. Insulin is responsible for facilitating the uptake of glucose into other tissues from the blood, as well as signaling whether to stimulate or slow glucose metabolism in the liver. Hyperglycemia is one of the main effects of diabetes, a disease characterized by the body’s inability to produce or respond to insulin. Without insulin, hepatic glucose output continues and blood glucose accumulates, causing …

The Short Allele Of The Serotonin Transporter Gene (Slc6a4) Increases Predisposition To Anxiety And Negative Emotional States, Gabriella Hitti

PANDION: The Osprey Journal of Research and Ideas

Mental disorders, such as anxiety and mood disorders, have long been a focus of psychological research, and increasing evidence points to a genetic basis for their occurrence. Polymorphisms of the serotonin transporter (5-HTT) gene (SLC6A4) may predispose an individual to anxiety-related symptoms. SLC6A4 has two common alleles: the short (s) allele, which results in less 5-HTT protein production, and the long (l) allele, which results in more 5-HTT. Integrated findings from psychometric evaluations, behavioral animal models, and biological assessments establish a link between the s allele of SLC6A4 and heightened anxiety phenotypes. The allele’s influence on serotonin levels and brain …

Basic Psychological Needs Satisfaction And Well-Being Among Seafarers: An Investigation Of Interaction Effects Through The Lens Of Self-Determination Theory, Nong Vuong Phi, Kung Don Ye, Feng Ming Tsai, Hsiao Chao Hsieh, Shiou-Yu Chen

Journal of Marine Science and Technology–Taiwan

Enhancing employee well-being is a primary goal for enterprise sustainable development. Although seafarers represent a major human resource in the maritime industry, the distinctive demands of their profession often compel them to compromise on certain elements in fulfilling their basic psychological needs, thereby contributing to mental health challenges. Recently, COVID-19 and its associated border control measures have prolonged the voyages of seafarers, drawing public attention to the topic of seafarer well-being. In this study, self-determination theory was used to explore the effects of autonomy, competence, and relatedness as fundamental psychological needs on seafarer well-being. After the independent effects of these …

Growth Of Passionflower (Passiflora Incarnata) On A Green Roof Using Commercial Fertilizer Vs. Natural Compost, Siobhan Day, Corinne Parker, Carmyn Harrison, Lauren York

Science University Research Symposium (SURS)

Passionflower (Passiflora incarnata), Tennessee’s state wildflower, is valued for its ecological role as a pollinator plant and its historical significance to the Cherokee, making it an ideal candidate for green roofs that support biodiversity and urban sustainability. This study examined the feasibility of growing passionflower on a green roof and tested whether commercial fertilizer (Miracle-Gro) or natural compost would lead to greater growth compared to no fertilizer. Twelve plants were divided equally into three treatment groups: control, Miracle-Gro, and compost, with growth measurements taken weekly from September to November. Results suggest that passionflowers treated with Miracle-Gro achieved the most growth, …

10.07.2024 Orsp Connect, Liz Williamson

ORED Newsletter

Lunch and Learn

Update Employee Directory

NIH Updates RPPR Instruction Guide

Conserved Signaling Modules Regulate Filamentous Growth In Fungi: A Model For Eukaryotic Cell Differentiation, Matthew D Vandermeulen, Michael C Lorenz, Paul J Cullen

Faculty, Staff and Student Publications

Eukaryotic organisms are composed of different cell types with defined shapes and functions. Specific cell types are produced by the process of cell differentiation, which is regulated by signal transduction pathways. Signaling pathways regulate cell differentiation by sensing cues and controlling the expression of target genes whose products generate cell types with specific attributes. In studying how cells differentiate, fungi have proved valuable models because of their ease of genetic manipulation and striking cell morphologies. Many fungal species undergo filamentous growth-a specialized growth pattern where cells produce elongated tube-like projections. Filamentous growth promotes expansion into new environments, including invasion into …

Creating Sustainable School And Home Gardens: Composting, Larry Krissek, Kathy Cabe Trundle, Rita Hagevik

All Current Publications

This fact sheet provides information about composting, such as what compost is, how to compost, and the benefits of composting in your garden.

An Iron-Rich Subset Of Macrophages Promotes Tumor Growth Through A Bach1-Ednrb Axis, Ian W Folkert, William A Molina Arocho, Tsun Ki Jerrick To, Samir Devalaraja, Irene S Molina, Hesham Mohei, Md Naushad Akhtar, Veena Kochat, Emre Arslan, Alexander J Lazar, Khalida Wani, William P Israel, Zhan Zhang, Venkata S Chaluvadi, Robert J Norgard, Ying Liu, Ashley M Fuller, Mai T Dang, Robert E Roses, Giorgos C Karakousis, John T Miura, Douglas L Fraker, T S Karin Eisinger-Mathason, M Celeste Simon, Kristy Weber, Kai Tan, Yi Fan, Kunal Rai, Malay Haldar

Faculty, Staff and Student Publications

We define a subset of macrophages in the tumor microenvironment characterized by high intracellular iron and enrichment of heme and iron metabolism genes. These iron-rich tumor-associated macrophages (iTAMs) supported angiogenesis and immunosuppression in the tumor microenvironment and were conserved between mice and humans. iTAMs comprise two additional subsets based on gene expression profile and location-perivascular (pviTAM) and stromal (stiTAM). We identified the endothelin receptor type B (Ednrb) as a specific marker of iTAMs and found myeloid-specific deletion of Ednrb to reduce tumor growth and vascular density. Further studies identified the transcription factor Bach1 as a repressor of the iTAM transcriptional …

Author Correction: Longitudinal Single-Cell Profiling Reveals Molecular Heterogeneity And Tumor-Immune Evolution In Refractory Mantle Cell Lymphoma, Shaojun Zhang, Vivian Changying Jiang, Guangchun Han, Dapeng Hao, Junwei Lian, Qingsong Cai, Joseph Mcintosh, Ruiping Wang, Minghao Dang, Enyu Dai, Yuanxin Wang, David Santos, Maria Badillo, Angela Leeming, Zhihong Chen, Kimberly Hartig, John Bigcal, Jia Zhou, Rashmi Kanagal-Shamanna, Chi Young Ok, Hun Lee, Raphael E Steiner, Jianhua Zhang, Xingzhi Song, Ranjit Nair, Sairah Ahmed, Alma Rodriquez, Selvi Thirumurthi, Preetesh Jain, Nicolaus Wagner-Bartak, Holly Hill, Krystle Nomie, Christopher Flowers, Andrew Futreal, Linghua Wang, Michael Wang

Faculty, Staff and Student Publications

No abstract provided.

Immunologic Signatures Of Response And Resistance To Nivolumab With Ipilimumab In Advanced Metastatic Cancer, Apostolia M Tsimberidou, Farah A Alayli, Kwame Okrah, Alexandra Drakaki, Danny N Khalil, Saad A Khan, David Y Oh, Christopher R Cabanski, Shikha Gautam, Stefanie L Meier, Meelad Amouzgar, Shannon M Pfeiffer, Robin Kageyama, Enjun Yang, Marko Spasic, Michael T Tetzlaff, Wai Chin Foo, Travis J Hollmann, Yanyun Li, Matthew Adamow, Phillip Wong, Jonni S Moore, Sharlene Velichko, Richard O Chen, Dinesh Kumar, Samantha Bucktrout, Ramy Ibrahim, Ute Dugan, Lisa Salvador, Vanessa M Hubbard-Lucey, Jill O'Donnell-Tormey, Sandra Santulli-Marotto, Lisa H Butterfield, Diane M Da Silva, Justin Fairchild, Theresa M Lavallee, Lacey J Padrón, Padmanee Sharma

Faculty, Staff and Student Publications

Identifying pan-tumor biomarkers that predict responses to immune checkpoint inhibitors (ICI) is critically needed. In the AMADEUS clinical trial (NCT03651271), patients with various advanced solid tumors were assessed for changes in intratumoral CD8 percentages and their response to ICI. Patients were grouped based on tumoral CD8 levels: those with CD8 <15% (CD8-low) received nivolumab (anti-PD-1) plus ipilimumab (anti-CTLA4) and those with CD8 ≥15% (CD8-high) received nivolumab monotherapy. 79 patients (72 CD8-low and 7 CD8-high) were treated. The disease control rate was 25.0% (18/72; 95% CI: 15.8–35.2) in CD8-low and 14.3% (1/7; 95% CI: 1.1–43.8) in CD8-high. Tumors from 35.9% (14/39; 95% CI: 21.8–51.4) of patients converted from CD8 <15% pretreatment to ≥15% after treatment. Multiomic analyses showed that CD8-low responders had an inflammatory tumor microenvironment pretreatment, enhanced by an influx of CD8 T cells, CD4 T cells, B cells, and macrophages upon treatment. These findings reveal crucial pan-cancer immunological features for ICI response in patients with metastatic disease.

Longitudinal Single-Cell Profiling Reveals Molecular Heterogeneity And Tumor-Immune Evolution In Refractory Mantle Cell Lymphoma, Shaojun Zhang, Vivian Changying Jiang, Guangchun Han, Dapeng Hao, Junwei Lian, Qingsong Cai, Joseph Mcintosh, Ruiping Wang, Minghao Dang, Enyu Dai, Yuanxin Wang, David Santos, Maria Badillo, Angela Leeming, Zhihong Chen, Kimberly Hartig, John Bigcal, Jia Zhou, Rashmi Kanagal-Shamanna, Chi Young Ok, Hun Lee, Raphael E Steiner, Jianhua Zhang, Xingzhi Song, Ranjit Nair, Sairah Ahmed, Alma Rodriquez, Selvi Thirumurthi, Preetesh Jain, Nicolaus Wagner-Bartak, Holly Hill, Krystle Nomie, Christopher Flowers, Andrew Futreal, Linghua Wang, Michael Wang

Faculty, Staff and Student Publications

The mechanisms driving therapeutic resistance and poor outcomes of mantle cell lymphoma (MCL) are incompletely understood. We characterize the cellular and molecular heterogeneity within and across patients and delineate the dynamic evolution of tumor and immune cell compartments at single cell resolution in longitudinal specimens from ibrutinib-sensitive patients and non-responders. Temporal activation of multiple cancer hallmark pathways and acquisition of 17q are observed in a refractory MCL. Multi-platform validation is performed at genomic and cellular levels in PDX models and larger patient cohorts. We demonstrate that due to 17q gain, BIRC5/survivin expression is upregulated in resistant MCL tumor cells and …

Cryo-Em Analysis Of Pseudomonas Phage Pa193 Structural Components, Stephano M. Iglesias, Chun-Feng Hou, Johnny Reid, Evan Schauer, Renae Geier, Angela Soriaga, Lucy Sim, Lucy Gao, Julian Whitelegge, Pierre Kyme, Deborah Birx, Sebastien Lemire, Gino Cingolani

Department of Biochemistry and Molecular Biology Faculty Papers

The World Health Organization has designated Pseudomonas aeruginosa as a critical pathogen for the development of new antimicrobials. Bacterial viruses, or bacteriophages, have been used in various clinical settings, commonly called phage therapy, to address this growing public health crisis. Here, we describe a high-resolution structural atlas of a therapeutic, contractile-tailed Pseudomonas phage, Pa193. We used bioinformatics, proteomics, and cryogenic electron microscopy single particle analysis to identify, annotate, and build atomic models for 21 distinct structural polypeptide chains forming the icosahedral capsid, neck, contractile tail, and baseplate. We identified a putative scaffolding protein stabilizing the interior of the capsid 5-fold …

Clustered Setation On The Pedipalps Of Buthid Scorpions (Scorpiones: Buthidae), Graeme Lowe, Victoria Tang

Euscorpius

Chaetotaxy of the external pedipalp femur and distal ventral pedipalp movable finger was studied in 120 species, 69 genera and 17 families of scorpions. Setation was generally denser in the ‘Buthus’ group, a major arid-adapted buthid lineage distributed across Palearctic deserts. On the external femur, macrosetae formed a prominent cluster, the ‘distal external macrosetal cluster’ (DEMC); on the distal ventral movable finger they formed a dense patch, the ‘distal ventral macrosetal cluster’ (DVMC). In other buthids and non-buthids, the DEMC and DVMC were mostly absent, except in a few arid-adapted genera. Relative setation densities of DEMC and DVMC in different …

Freshwater Mussels As High-Resolution Recorders Of Local Climate Data: Learning From An Imperiled Species, Mia Meighan, Linh Truong, Tristan Keane, Jens Hegg, Joel Saunder, Casey Mccormack

CURCI Student Research

Freshwater mussels are native bivalve mollusks and an important indicator species for water quality. Over 65% of the species present in North America are imperiled.

In the West, mussel populations are generally declining, and the western ridged mussel (Gonidea angulata), native to the Pacific Northwest, is under review by the US Fish & Wildlife Service for Endangered Species Act listing.

Just as scientists use dendrochronology to determine the climate conditions of a forest using tree rings, sclerochronology can be used to determine the condition of stream habitats using growth bands stored within mussel shells.

Histological Characterization Of Larch Mountain Salamander (Plethodon Larselli) Head Glands, Jaxon Hopkins, Nancy Staub

CURCI Student Research

In salamanders, successful reproduction requires communication. Most male salamanders communicate to the female during courtship via a gland on their chin, called the mental gland. The sexually dimorphic mental gland in the dermis of the lower jaw is typical in males of species in the family Plethodontidae and is considered ancestral to the family (Sever et al. 2016). The mental gland consists of an aggregate of modified granular glands (MGGs) that produce pheromones (Houck and Sever, 1994; Rupp and Sever, 2018). During courtship, pheromones secreted from mental glands are delivered to the female through head rubbing and biting (Houck and …