Life Sciences Commons^™

Extraction Method Effects On Structural Properties And Functional Characteristics Of Dietary Fiber Extracted From Ginseng Residue, Xiaoyu Feng, Kashif Ameer, Karna Ramachandraiah, Guihun Jiang

School of Medicine Faculty Publications

In this research, the dietary fibers (DFs) from ginseng residue were extracted by employing three different extraction methods (alkaline: AL, acidic: AC, enzymatic: EN). The extracted DFs were characterized in terms of their structural and functional properties. The results clearly showed that, regardless of the extraction methods, all DF samples exhibited representative infrared spectral features. The DF extracted by AC (citric acid) had more porous structures with a looser configuration, in conjunction with high apparent viscosity, whereas the DF extracted by EN (α-amylase and protease) exhibited higher thermal stability. Moreover, the monosaccharide composition of the DF samples was significantly influenced …

Elucidating The Heterogeneity And Epigenetic Determinants Of Memory-Like Natural Killer Cell Differentiation, Jennifer Tran

Arts & Sciences Graduate Student Theses and Dissertations

Natural killer (NK) cells are innate lymphoid cells that are a first line of defense against virally infected or malignant cells. Their ability to recognize and kill target cells is controlled by engagement of inhibitory, activating, and cytokine receptors. This leads to release of granules that contain perforin and granzymes, expression of death receptor ligands, and production of cytokines and chemokines. An effective NK cell response results in target cell death, along with changes in the nearby cellular microenvironment to promote immunity. As such NK cells play an essential role in tumor immunosurveillance, and there is an inverse correlation between …

Elucidating The Heterogeneity And Epigenetic Determinants Of Memory-Like Natural Killer Cell Differentiation, Jennifer Tran

Arts & Sciences Graduate Student Theses and Dissertations

Natural killer (NK) cells are innate lymphoid cells that are a first line of defense against virally infected or malignant cells. Their ability to recognize and kill target cells is controlled by engagement of inhibitory, activating, and cytokine receptors. This leads to release of granules that contain perforin and granzymes, expression of death receptor ligands, and production of cytokines and chemokines. An effective NK cell response results in target cell death, along with changes in the nearby cellular microenvironment to promote immunity. As such NK cells play an essential role in tumor immunosurveillance, and there is an inverse correlation between …

Memory Modulation: Dominance Of Negative Visual Context Over Neutral Verbal Memory, Stas Kozak, Noa Herz, Maya Tocker, Yair Bar-Haim, Nitzan Censor

Department of Neurology Faculty Papers

Neutral memories can be modulated via intentional memory control paradigms such as directed forgetting. In addition, previous studies have shown that neutral visual memories can be modulated indirectly, via remember and forget instructions towards competing verbal memories. Here we show that direct modulation of neutral verbal memory strength is impaired by negative visual context, and that negative visual context is resistant to indirect memory modulation. Participants were directly instructed to intentionally remember or forget newly encoded neutral verbal information. Importantly, this verbal information was interleaved with embedded negative visual context. Results showed that negative visual context eliminated the well-documented effect …

Creating Sustainable School And Home Gardens: Wildlife Monitoring Using Bioacoustics, Kaitlin Campbell, Rita Hagevik, Kathy Cabe Trundle

All Current Publications

Animals use sounds to communicate with each other, just like humans. Humans can listen in on animals by using a bioacoustic monitoring device, a passive device that quietly records surrounding sounds. Bioacoustic monitoring devices blend in with the environment, allowing us to know what types of animals appear when we’re gone. The device collects and stores data and allows us to visualize, analyze, and identify animals from the sounds they make.

Creating Sustainable School And Home Gardens: Using Weather Stations, Jake Sanders, Daniel Findlay, Kathy Cabe Trundle, Rita Hagevik, Kaitlin Campbell

All Current Publications

This fact sheet provides an overview of how weather stations work and the steps to set up your own weather station. To help monitor the water conditions, wind speeds, and temperatures that can impact your plants, we suggest adding a weather station to your garden area or greenhouse.

Ovarian Cancer Metastasis: Looking Beyond The Surface, Emine Bayraktar, Sisy Chen, Sara Corvigno, Jinsong Liu, Anil K Sood

Faculty, Staff and Student Publications

Historically, ovarian cancer (OC) was thought to metastasize by surface-to-surface spread, but recent developments have yielded a new understanding of the paths of metastatic spread. Given the histologic and molecular heterogeneity of OC, we will focus on high-grade serous carcinoma (HGSC). Here, we provide a critical and more holistic view of the evidence supporting various routes of metastasis, including peritoneal, hematogenous, lymphatic, and nerve-related. Understanding the underlying mechanisms is necessary to improve treatment strategies for this challenging disease.

Left Ventricular Diastolic Dysfunction With Elevated Filling Pressures Is Associated With Embolic Stroke Of Undetermined Source And Atrial Fibrillation, Zubair Bashir, Liqi Shu, Yuqian Guo, Edward W Chen, Shuyuan Wang, Maheen Rana, Xing Dai, Daniel Mandel, Shadi Yaghi, Phinnara Has, Mingxing Xie, Tao Wang, James Simmons, Christopher Song, Philip Haines

Faculty, Staff and Student Publications

Background/Objectives: Left ventricular diastolic dysfunction (LVDD) and elevated left ventricular filling pressure (LVFP) are strong predictors of clinical outcomes across various populations. However, their diagnostic utility in embolic stroke of undetermined source (ESUS) remains unclear. We hypothesized that LVDD with elevated LVFP (based on echocardiography) was more likely to be prevalent in ESUS compared to non-cardioembolic stroke (NCE) and to be associated with atrial fibrillation (AF) on follow-up monitoring.

Methods: This is a single-center retrospective study that included adult patients with a diagnosis of acute ischemic stroke between January 2016 and June 2017. LV function was assessed by …

The Role Of Jordanian Television Programs In Raising Awareness About Mental Health From The Perspective Of Psychiatrists, Yasmine Hamza Badis, Saddam Suleiman Salman Almashaqbeh

The study aimed to identify the role of Jordanian television programs in raising awareness of mental health from the perspective of psychiatrists. The sample of the study was composed of 153 members from the Jordanian Association of Psychiatrists affiliated with the Jordanian Medical Association. To achieve the goal of this study, the descriptive approach was followed, and the study relied on the questionnaire as a tool for collecting data. The results of the study showed that the role of Jordanian television programs in raising awareness about mental health from the Perspective of psychiatrists was medium, and the diversity of features …

Effects Of Cadherin Mediated Contact Normalization On Oncogenic Src Kinase Mediated Gene Expression And Protein Phosphorylation, Rachel E Nicoletto, Cayla J Holdcraft, Ariel C Yin, Edward P Retzbach, Stephanie A Sheehan, Amanda A Greenspan, Christopher M Laugier, Jason Trama, Caifeng Zhao, Haiyan Zheng, Gary S Goldberg

Rowan-Virtua School of Osteopathic Medicine Departmental Research

Nontransformed cells form heterotypic cadherin junctions with adjacent transformed cells to inhibit tumor cell growth and motility. Transformed cells must override this form of growth control, called "contact normalization", to invade and metastasize during cancer progression. Heterocellular cadherin junctions between transformed and nontransformed cells are needed for this process. However, specific mechanisms downstream of cadherin signaling have not been clearly elucidated. Here, we utilized a β-catenin reporter construct to determine if contact normalization affects Wnt signaling in transformed cells. β-catenin driven GFP expression in Src transformed mouse embryonic cells was decreased when cultured with cadherin competent nontransformed cells compared to …

Bi-Directional Allosteric Pathway In Nmda Receptor Activation And Modulation, Paula A Bender, Subhajit Chakraborty, Ryan J Durham, Vladimir Berka, Elisa Carrillo

Faculty, Staff and Student Publications

N-methyl-D-aspartate (NMDA) receptors are ionotropic glutamate receptors involved in learning and memory. NMDA receptors primarily comprise two GluN1 and two GluN2 subunits. The GluN2 subunit dictates biophysical receptor properties, including the extent of receptor activation and desensitization. GluN2A- and GluN2D-containing receptors represent two functional extremes. To uncover the conformational basis of their functional divergence, we utilize single-molecule fluorescence resonance energy transfer to probe the extracellular domains of these receptor subtypes under resting and ligand-bound conditions. We find that the conformational profile of the GluN2 amino-terminal domain correlates with the disparate functions of GluN2A- and GluN2D-containing receptors. Changes at the pre-transmembrane …

Scaling Individual Tree Transpiration With Thermal Cameras Reveals Interspecies Differences To Drought Vulnerability, Mostafa Javadian, Donald M. Aubrecht, Joshua B. Fisher, Russell L. Scott, Sean P. Burns, Jen L. Diehl, J. William Munger, Andrew D. Richardson

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Understanding tree transpiration variability is vital for assessing ecosystem water-use efficiency and forest health amid climate change, yet most landscape-level measurements do not differentiate individual trees. Using canopy temperature data from thermal cameras, we estimated the transpiration rates of individual trees at Harvard Forest and Niwot Ridge. PT-JPL model was used to derive latent heat flux from thermal images at the canopy-level, showing strong agreement with tower measurements (R2 = 0.70–0.96 at Niwot, 0.59–0.78 at Harvard at half-hourly to monthly scales) and daily RMSE of 33.5 W/m2 (Niwot) and 52.8 W/m2 (Harvard). Tree-level analysis revealed species-specific responses to …

My Family Screwed Me Up: Adverse Childhood Experiences, Romantic Relationships, And The Healing Role Of Resilience, Arianna Elmer

Family Perspectives

No abstract provided.

Raising Heroes: Transforming Homes Into Media Training Grounds, Elizabeth Rex Johnson

Family Perspectives

No abstract provided.

Combating Social Media’S Effect On Body Image: A Father’S Role, Brooke Ashcraft

Family Perspectives

No abstract provided.

Early, Open, And Frequent Parent-Child Sexual Communication And Teen Sexual Health, Rachel Shumway

Family Perspectives

No abstract provided.

How The Family Of Origin’S Belief System Can Affect Decision Making In Emerging Adulthood, Savannah Lowe

Family Perspectives

No abstract provided.

Parenting Under Pressure: How Socioeconomic Status And Stress Affect Parenting, Lynden Wagner

Family Perspectives

No abstract provided.

Development Of A High-Density Genetic Linkage Map And Identification Of Quantitativetrait Loci (Qtls) Associated With Botrytis Cinerea Resistance In Strawberry(Fragaria × Ananassa Duch.), Duygu Ayvaz Sönmez, Harun Karci, Hayat Topçu, Ömer Faruk Bi̇lgi̇n, Hale Günaçti, Habi̇bullah Tevfi̇k, Burak Özgören, Yefim Ronin, Elena Baraldi, Sali̇h Kafkas, Nesi̇be Ebru Kafkas

Turkish Journal of Agriculture and Forestry

Over the centuries, strawberry fruit quality has greatly improved through various selection studies. The discovery of genes andgenomic positions related to traits such as fruit quality and resistance to diseases and pests has been accelerated by new biotechnologicalmethods. The objectives of this study are (i) to construct single nucleotide polymorphism (SNP) Fana array-based high-throughputgenetic linkage groups for the ‘Fortuna × Rubygem’ F1 population, and (ii) to detect quantitative trait loci (QTL) regions associated withBotrytis cinerea resistance in leaf and fruit traits of strawberry. A total of 945 and 984 SNPs were mapped in the ‘Fortuna’ maternal andthe ‘Rubygem’ paternal maps, …

Change In Plant Growth And Some Bioactive Components Of The Tomato Under Water Deficit With Exogenous Chrysin Treatment, Melek Eki̇nci̇, Ertan Yildirim, Selda Örs, Meti̇n Turan, Duran Şi̇mşek, Yüsra İnci̇

Turkish Journal of Agriculture and Forestry

Drought is one of the important factors that cause a decrease in productivity and quality in plant cultivation. Stress tolerance in plants can be increased or damage can be reduced by various exogenous applications. One of them is flavonoids, which have an important role in the response to stress through changes that occur at the endogenous level in the plant due to drought stress. The effects of exogenous treatment of chrysin, a member of the flavonoid group, on tomato seedlings grown under water deficit were examined. Different doses (C0: Control with no treatment, C1: 0.1 mM, C2: 0.5 mM and …

Ex Vivo And In Vitro Methods As A Platform For Studying Anthropogenic Effects On Marine Mammals: Four Challenges And How To Meet Them, Juan M. Vazquez, Jane I. Khudyakov, Carla B. Madelaire, Céline A. Godard-Codding, Heli Routti, Emily K. Lam, Elizabeth R. Piotrowski, Greg B. Merrill, Jillian H. Wisse, Kaitlin N. Allen, Justin Conner, Pierre Blévin, Demetri D. Spyropoulos, Anders Goksøyr, José P. Vázquez-Medina

Pacific Faculty Work

Marine mammals are integral to global biodiversity and marine health through their roles in coastal, benthic, and pelagic ecosystems. Marine mammals face escalating threats from climate change, pollution, and human activities, which perturb their oceanic environment. The diverse biology and extreme adaptations evolved by marine mammals make them important study subjects for understanding anthropogenic pressures on marine ecosystems. However, ethical and logistical constraints restrict the tractability of experimental research with live marine mammals. Additionally, studies on the effects of changing ocean environments are further complicated by intricate gene-environment interactions across populations and species. These obstacles can be overcome with a …

Quality Characteristics Of The Cv. Albion Strawberry (Fragaria X Ananassa Duch.)In Different Locations, Burhanetti̇n İmrak, Ayşegül Esra Gölcü, Songül Çömlekçi̇oglu, Mehmet Ramazan Bozhüyük, Jiri Mlcek, Gürsel Özkan, Sona Skrovankova

Turkish Journal of Agriculture and Forestry

Strawberries are one of the most popular berries in the world due to their distinctive aroma, flavor, and known healthproperties. A top day-neutral strawberry variety, the Albion, with its potential for high yields of large fruit, was grown in two differentlocations (Dağdibi and Hamidiye) at different altitudes (1410 and 1293 m) in Pozantı, a district of the Adana province in Türkiye, for lateseason production. Ripe fruits were harvested during the commercial harvest period, and several important physical and biochemicalparameters were examined. In addition, the fruit’s (berry’s) external and flesh color, fruit length and width, soluble solid content (SSC),antioxidant capacity, total …

Significance Of Honeybee Pollination In Increasing Seed Yield Of Trifolium Alexandrinum (Fabales: Fabaceae) And Its Impact On Economic Sustainability Of Smallholder Farmers, Muhammad Shoaib Tufail, Gaye L. Krebs, Alison Southwell, John Piltz, Peter C. Wynn, David F. Cook

Grain and Other Field Crops Research Articles

A major limitation to producing high seed yields in berseem clover (Trifolium alexandrinum L.) is failure to set seed, predominantly due to lack of pollination. Despite the importance of berseem clover as a leading forage legume, the contribution of pollinators to seed set is scarce. In Pakistan, the honeybee population is declining mainly because of the extensive use of neonicotinoid pesticides and habitat fragmentation. This, combined with the region’s harsh environment and the use of inferior, locally bred genotypes, has resulted in low seed yields. Insufficient seed availability leads to limited forage supply, resulting in poor livestock nutrition, which …

Shell Translucency: A Novel Eggshell Quality Parameter In Broiler Breeders, Duarte Neves, Austin Jasek, Colwayne Morris, Raquel Burin, Kevin Stephens, Marco Rebollo

Proceedings of the Arkansas Nutrition Conference

In the United States, nearly 240 million commercial broiler breeder eggs are incubated weekly with an average hatchability of 79.1% (Third Party Reporting). Between 160 eggs/hen and 125 chicks/hen the loss is considerable. In 2007, an estimated 1% improvement in hatchability would escalate profits by over $25 million to hatcheries and markedly increase profitability in broiler operations (Schaal and Gherian, 2007). In order to tap into top hatchability performances, egg management in commercial hatcheries such as egg storage time and conditions, incubation temperature and humidity, as well as turning frequency are of utmost importance (Vick et al., 1993; Reis et …

Working In Pairs Increases Consistency Of Georgia Adopt-A-Stream's Habitat Assessment, Christopher H. Kodani, Robert Bourne, Cody Jones, Michael Kahle, Cecilia Nachtmann

Georgia Journal of Science

Habitat assessment is a skill that is important to stream ecologists of all levels, including volunteers of water quality monitoring programs such as Georgia Adopt-A-Stream (AAS). Some aspects of habitat assessment can be subjective because observers must use their best judgement in assessing some variables, therefore, it is worthwhile to examine the variation in volunteer stream monitor observations, the effect of training, and the reliability of habitat metrics calculated from the data collected. To gain some insight, we asked volunteers attending a workshop at AAS’s 2022 Confluence Conference to complete a Habitat Assessment Worksheet both before and after a short …

Intelligrader: A Framework For Automatic Short Answer Grading, Inconsistency Check And Feedback In Educational Context - Conception, Implementation And Evaluation, Paradesi Sree Lakshmi, Jay B. Simha, Rajeev Ranjan

Karbala International Journal of Modern Science

Automatic Short Answer Grading (ASAG), an escalating realm in natural language understanding, constitutes a focal point of research within the broader field of learning analytics. Over time, many ASAG solutions have been proposed to address the difficulties in teaching. However, no work addressed three crucial aspects of evaluation together, i.e., i) automatic evaluation of brief subjective/descriptive answers written in English, ii) identifying the evaluation inconsistency, and iii) provision of providing feedback about inconsistent evaluation to the evaluator. The current work proposes IntelliGrader, a comprehensive ASAG system that addresses the above-mentioned issues. Automated grading is accomplished through a model answer-based approach. …

Isolation And Characterization Of Fermenting Bacterial Isolates From Vinegar Industry Waste In Local Markets Of Wasit Province, Tayseer Shamran Al-Deresawi

Karbala International Journal of Modern Science

The use of waste in the vinegar industry is an important practice of the food industry worldwide; however, this critical practice is neglected in many parts of Iraq. According to this, the current study was conducted to isolate and characterize fermenting bacterial organisms from this waste in Wasit Province, Iraq. Samples of vinegar industry by-products were collected from local businesses. These samples were prepared by using Hestrin-Schramm (HS) medium. Two methods, direct and indirect inoculations, were followed. The purified growth was examined using morphological and biochemical tests. Moreover, PCR was employed to confirm the identity of each bacterial isolate. The …

The Heart-Brain Axis: Exploring Shared Genetic Signatures And Environmental Modifiers On Heart Failure And Alzheimer’S Disease Pathology, Helen Butler

MUSC Theses and Dissertations

Heart failure (HF) and Alzheimer’s Disease (AD) are two leading causes of death in the United States. Conventionally viewed as independent conditions, HF/AD coexist in ~30% of patients and are mechanistically linked by shared genetic and biochemical characteristics. The del Monte lab has established the concept of the heart-brain axis through human samples and murine models, providing a framework for this dissertation which focuses on the interplay between genetics and the environment in shaping the heterogeneity of disease phenotypes.

1) Loss of function mutations in presenilin (PSEN1/2) genes represent overlapping genetic signatures in AD/HF. Presenilins form the g-secretase …

A Receptor For Dual Ligands Governs Plant Immunity And Hormone Response And Is Targeted By A Nematode Effector, Li Huang, Yulin Yuan, Chloe Ramirez, Zhe Zhao, Letien Chen, Thomas Griebl, Joanna Kud, Joseph C. Kuhl, Allan Caplan, Louise-Marie Dandurand, Fangming Xiao

Entomology and Plant Pathology Faculty Publications and Presentations

Significance

Abstract

Qualitative Literacy: A Guide To Evaluating Ethnographic And Interview Research, Frida Akmalia

The Qualitative Report

Qualitative Literacy: A Guide to Evaluating Ethnographic and Interview Research by Mario Luis Small and Jessica McCrory Calarco is a comprehensive guide that offers a framework for evaluating the quality of qualitative research. The book is particularly relevant for researchers seeking to assess or enhance the rigor of their qualitative studies. Small and Calarco (2022) introduce five key indicators; cognitive empathy, heterogeneity, palpability, follow-up, and self-awareness that assist readers and researchers in determining whether a qualitative study is well-executed. This review discusses the organization and content of the book, the rationale for selecting it, its strengths and weaknesses, and provides …