Life Sciences Commons^™

Extraction Of Anthocyanins From Purple Sweet Potato Using Supercritical Carbon Dioxide And Conventional Approaches, Gabriel Laquete De Barros, Arda Tuhanioglu, Sumanjot Kaur, Leonardo Lora, Ali Ubeyitogullari

Food Science Faculty Publications and Presentations

This research investigates the potential of supercritical carbon dioxide (SC-CO₂) technology as a green and sustainable approach to improve anthocyanin extraction from purple sweet potatoes (PSP). The study explored different extraction parameters (i.e., temperatures of 35–55 °C, cosolvent concentrations of 10–30 % (w/w), ethanol ratios of 30–70 % (v/v), pressures of 30–40 MPa, extraction times of 120–180 min, and solvent: sample ratios of 25:1–45:1(v/w)), to optimize the total phenolic content (TPC), anthocyanin content (ANC), and antioxidant activity (AA). Additionally, conventional extraction methods using different solvent (ethanol and methanol) mixtures, solvent: sample ratios (15:1; 45:1; 6:1 (v/w)), temperatures (35–50 …

Investigation Of Reproductive Inhibition During Chronic Heat Stress In C. Elegans, Macy Makenna Nielander

Theses and Dissertations

Nearly all organisms experience heterogeneity in environmental conditions that can negatively impact fitness. A common adaptive strategy to reduce the impact of this stress is called facultative diapause, where development and reproduction are temporarily arrested to promote survival. Facultative diapause is characterized by three major criteria, the arrest must be (i) induced by an unfavorable environmental condition, (ii) reversible, and (iii) driven by genetically regulated mechanisms. The aim of this thesis was to determine whether chronic heat-induced inhibition of egg laying in Caenorhabditis elegans represents a novel form of facultative diapause. It was found that the inhibition of egg-laying is …

Preparing Educators To Effectively Engage With Postsecondary Stem Students With Autism, Luzconsuelo Gavaldon

Open Access Theses & Dissertations

Autism spectrum disorder (ASD) is a lifetime mental disability (Boucher, 2009). Because the brains of people with autism work differently than their non-neurodiverse peers (Kuzminskaite et al., 2020), they tend to respond to external stimuli in a hypo- or hyper-sensory fashion. One of the primary considerations that must be accounted for when working with individuals with autism is that the brains of these persons are each neurologically distinct. In other words, there is no "one-size-fits-all" means by which individuals with ASD learn and develop and, consequently, no single way to therefore engage with said individuals (Anderson & Butt, 2017; Kuzminski …

Seasonal Abundances Of Aedes Aegypti And Culex Quinquefasciatus In An Urban Community In The Texas/Mexico Border, Kevyn Amezcua

Open Access Theses & Dissertations

AAedes aegypti and Culex quinquefasciatus are two medically relevant species of mosquitoes that can transmit several arboviruses that affect human health. Ae. aegypti is a vector for viral diseases such as Dengue, Yellow Fever, Chikungunya, and Zika, while Cx. quinquefasciatus is a vector for West Nile and Saint Louis Encephalitis. Both species are mostly found in urban environments and are affected by several anthropogenic factors such as the presence of artificial containers and overall changes to the native landscape. Little research is done on the ecology of these two species in the Chihuahuan desert, which has some areas that are …

Evolutionary History Of A Specialized And Diverse Highland Snake Complex (Crotalus Willardi), Michael Joseph Buontempo

Open Access Theses & Dissertations

The Ridgenosed rattlesnake (Crotalus willardi) is a highly unique group of small rattlesnakes consisting of 5 subspecies that inhabit the high elevation pine oak woodlands of the Sierra Madre Occidental and the Madrean Archipelago. They remain one of the most understudied groups of rattlesnakes with little understanding of the relationships between the subspecies. I utilize genomic level genetic data collected using ddRAD-seq methodology on 60 individuals encompassing all 5 subspecies and the majority of the range. Utilizing co-ancestry analysis, Admixture, and Phylogenetic analyses (both maximum likelihood and Bayesian approaches) we uncovered seven separate genetic groups. Of these, three consisted of …

Implementing The Methodology To Generate Diagnostic And Therapeutic Antibodies, Andrea Mariana Carlin

Open Access Theses & Dissertations

This research aimed to establish the methodology for developing monoclonal antibodies at The University of Texas at El Paso. Monoclonal antibodies are indispensable across multiple fields, including diagnostics, therapeutics, and biotechnology, yet there is a shortage of commercially available antibodies targeting specific proteins or possessing specialized characteristics. To address this gap, three models were selected: a peptide derived from the intracellular protein Schlafen 8 and two cell surface protein receptors: mouse vascular endothelial growth factor receptor 2 (VEGFR2) and human melanocortin receptor 4 (MC4R). These proteins present unique challenges in antibody generation, including issues of accessibility, expression, and specificity.

Throughout …

Examination Of The Role Of Ovarian Hormones On Nicotine Withdrawal Severity In Females, Priscilla Giner

Open Access Theses & Dissertations

Women are more susceptible to the long-term consequences of chronic nicotine exposure, and clinical evidence has shown that abstinence from nicotine exposure produces a withdrawal syndrome that is intensified by estrogen (E2 in rats) and progesterone (PR) in females. The goal of this Masterâ??s thesis was to determine the mechanisms by which E2 and PR promote withdrawal from nicotine vapor inhalation in female rats. Prior work in male rodents has revealed that nicotine withdrawal is modulated in the medial habenula-interpeduncular nucleus (MHb-IPN) pathway. Our hypothesis is that females experience greater inhibition of the IPN via local interneurons that release the …

Elevation And Population Size Effects On The Survival Of Drosera Rotundifolia, Jessica Williams

Electronic Theses and Dissertations

Carnivorous plants face significant conservation challenges due to habitat destruction, climate change, and illegal harvesting. This study explores how elevation and population size influence key-life history traits of D. rotundifolia, such as flowering phenology, prey capture efficiency, seed production, and germination rates. Higher elevations and larger populations are associated with extended flowering durations and increased prey capture. However, seed production and germination rates show complex patterns that are likely influenced by genetic diversity, resource availability, or environmental conditions. These insights inform targeted conservation strategies, emphasizing habitat preservation, enhancing prey availability, and maintaining genetic diversity. Public education and community engagement …

The Bacterial Cell Wall Components Lipopolysaccharide And Peptidoglycan Initiate Divergent Local Tissue And Systemic Inflammatory Response Profiles In The Chicken Model, Kristen A. Byrne, Gisela F. Erf

Poultry Science Faculty Publications and Presentations

The innate immune system plays an important role in the early defense against pathogens. To better understand the chicken’s innate inflammatory responses, we concurrently examined the cellular responses at the injection site and in the blood following intradermal (i.d.) injections of lipopolysaccharide (LPS) or peptidoglycan (PGN) into the pulp of growing feathers (GFs). Both LPS from Gram-negative bacteria and PGN from Gram-positive bacteria are potent stimulators of innate inflammatory responses. The time-course study conducted revealed similarly elevated levels of heterophils and macrophages in injected GF-pulps during the first 24 h for both LPS and PGN. However, PGN also stimulated rapid …

Neural Network Architecture Search Enabled Wide-Deep Learning (Nas-Wd) For Spatially Heterogenous Property Awared Chicken Woody Breast Classification And Hardness Regression, Chaitanya Pallerla, Yihong Feng, Casey M. Owens, Ramesh Bahadur Bist, Siavash Mahmoudi, Pouya Sohrabipour, Amirreza Davar, Dongyi Wang

Poultry Science Faculty Publications and Presentations

Due to intensive genetic selection for rapid growth rates and high broiler yields in recent years, the global poultry industry has faced a challenging problem in the form of woody breast (WB) conditions. This condition has caused significant economic losses as high as $200 million annually, and the root cause of WB has yet to be identified. Human palpation is the most common method of distinguishing a WB from others. However, this method is time-consuming and subjective. Hyperspectral imaging (HSI) combined with machine learning algorithms can evaluate the WB conditions of fillets in a non-invasive, objective, and high-throughput manner. In …

Characterization Of An Acetogenin-Carrying Nanosuspension And Its Effect On Bacteria Of Interest In The Poultry Industry, Brandon A. Lopez-Romero, Gabriela Aguilar-Hernandez, Billy M. Hargis, Maria De Lourdes Garcia-Magana, Ulises M. Lopez-Garcia, Rosa I. Ortiz-Basurto, Adalberto Zamudio-Ojeda, Juan D. Latorre, Efigenia Montalvo-Gonzalez

Poultry Science Faculty Publications and Presentations

This work aimed to develop a nanosuspension (NSps) as an acetogenin (ACGs) carrier, using soy lecithin (SL) and hydroxypropyl-β-cyclodextrin (βCD) named NSps-βCDSL-ACGs. It was characterized by various spectroscopic techniques (DLS, FTIR, UV-vis diffuse reflectance). Moreover, the NSps morphology was observed by transmission electron microscopy (TEM). Also, the antibacterial activity of NSps-βCDSL-ACGs was evaluated against strains of interest in the poultry sector. NSps-βCDSL-ACGs presented nanometric size (207–239 nm), acceptable polydispersity index (PDI) values (0.13–0.17) and a high Z potential value (−47.17–50.36 mV), demonstrating high stability. The presence of ACGs in NSps-βCDSL-ACGs was confirmed by FTIR analysis. The nanoparticles had a spherical …

Quantitative Analysis Of Protective Effect Of Resveratrol Against Nanoplastic-Induced Oxidative Stress, Bryan Gustafson

All Graduate Reports and Creative Projects, Fall 2023 to Present

Nanoplastics and microplastics are a growing ecological and health concern due to their widespread prevalence. The effects of these microscopic particles in biological systems primarily relate to their potential to produce reactive oxygen species and interactive effects with other cytotoxic agents. Of particular interest in this work is the effect on mammalian intestinal cells, due to the regular unintentional ingestion of micro- and nanoplastics in the normal western diet. In this study, Caco-2 cells were cultured and exposed to polystyrene particles in vitro as a simple model of the human digestive endothelium.

Resveratrol, a naturally occurring polyphenolic compound, has been …

Reducing Dna Extraction Costs Through Factorial Design For The Dnadvance Kit, Carson J. Neal, Zachery D. Zbinden, Michael E. Douglas, Marlis R. Douglas

Biological Sciences Faculty Publications and Presentations

Objective

Extracting DNA is essential in wildlife genetic studies, and numerous methods are available. However, the process is costly and time-consuming for non-model organisms, including most wildlife species. Therefore, we optimized a cost-efficient protocol to extract DNA from the muscle tissue of White-tailed Deer using the DNAdvance kit (Beckman Coulter), a magnetic-bead-based approach. We devised a 3 × 3 factorial design using combinations of tissue mass (10 mg, 50 mg, or 100 mg) and reaction volume (25%, 33%, and 50% of the manufacturer's recommended volumes). DNA was extracted for N = 81 tissue sub-samples (9 replicates/treatment).

Results

Our target yield …

Environmental Light Controls The Daily Organization Of Breathing By Activating Brn3b-Expressing Intrinsically Photosensitive Retinal Ganglion Cells In Mice, Aaron A. Jones, Allison R. Spears, Deanna M. Arble

Biological Sciences Faculty Research and Publications

Rhythmic, daily fluctuations in minute ventilation are controlled by the endogenous circadian clock located in the suprachiasmatic nucleus (SCN). While light serves as a potent synchronizer for the SCN, it also influences physiology and behavior by activating Brn3b-expressing, intrinsically photosensitive retinal ganglion cells (ipRGCs). It is currently unclear the extent to which the external light environment shapes daily ventilatory patterns independent of the SCN. To determine the relative influence of environmental light versus circadian timing on the organization of daily rhythms in minute ventilation, we used whole-body plethysmography to measure the breathing of mice housed on a non-entraining T28 cycle …

North Dakota Corn Efficiency Frontier: Stochastic Frontier Analysis, Bayarbat Badarch, David C. Roberts, Michael P. Popp

Agricultural Economics and Agribusiness Faculty Publications and Presentations

According to climate studies in North Dakota, the state's crop-growing season has been extended. In addition, many studies have shown technological advances in crop production. However, the state has not addressed how crop yield has been affected by weather changes. Thus, this paper investigates the state's corn (Zea mays) yield potential and efficiency measures based on agricultural input use and weather variables from 1994 to 2018. We found that the effects of temperature and precipitation on the state's corn yield frontier (potential) were greater than those of changing agricultural input variables. The stochastic frontier model indicates that the …

A Historical Assessment Of The Number Of Days Required To Plant The Arkansas Soybean Crop, Bayarbat Badarch, K. Bradley Watkins, Terry Griffin

Agricultural Economics and Agribusiness Faculty Publications and Presentations

The primary objective of this paper is to quantitatively analyze the number of acres planted per suitable fieldwork day and the total number of fieldwork days available for the complete planting of soybeans (Glycine max L.) in Arkansas. Total days to plant soybeans in each spring from 1980 to 2023 averaged about 33 days, indicating that roughly 33 days are expected, on average, from the month of April to May based on USDA-NASS weekly crop progress and condition data. Furthermore, the average soybean planted acres is estimated at 106,736 acres per day within the study period. Recently, variations in …

Economic Evaluation Of Grazing Steers On Summer Annuals Sod-Seeded Into Warm-Season Perennial Pasture, Ivanelle Meyer, Michael P. Popp, Christine C. Nieman, James L. Mitchell, Kenneth P. Coffey

Agricultural Economics and Agribusiness Faculty Publications and Presentations

Objectives

Materials and Methods

Results and Discussion …

Demand For Milk And Milk Products In The Rural Household Of Bangladesh: A Panel Data Analysis, Eshrat Jahan Mahfuza, Mohammad Jahangir Alam, Andrew M. Mckensie

Agricultural Economics and Agribusiness Faculty Publications and Presentations

Milk is considered an essential source of protein for people of all ages. However, Bangladeshi people, particularly those living in rural areas, tend to consume less milk and milk products than necessary. Therefore, this study aims to investigate the demand for milk and milk products among rural consumers and the factors affecting their consumption habits. The study utilizes balanced panel data from three waves of the Bangladesh Integrated Household Survey: 2011–12, 2015, and 2018–19, collected by the International Food Policy Research Institute. Both the Almost Ideal Demand System (AIDS) and Fixed Effects Model were employed. The expenditure elasticity for liquid …

Use Of A Capsule Suspension Formulation Of S-Metolachlor In Fenclorim-Treated Rice, Jason K. Norsworthy, Samuel C. Noe, Thomas R. Butts, Trenton L. Roberts

Crop, Soil and Environmental Sciences Faculty Publications and Presentations

As herbicide resistance continues to render commonly used rice herbicides ineffective, alternative sites of action are paramount to maintaining yield and producer profitability. Combining a slow-release formulation and a fenclorim seed treatment might allow the safe use of S-metolachlor in rice. Experiments were initiated in 2022 and 2023 near Colt, AR, on a silt loam soil to evaluate crop safety using a capsule suspension (CS) formulation of S-metolachlor and a fenclorim seed treatment in rice. The first experiment assessed the tolerance of two cultivars (‘Diamond’ and ‘DG263L’) to three rates (0.42, 0.84, and 1.68 kg ai ha^{−1 …}

Tc-Ptp-Mediated Regulation Of P38 Mapk Pathway Promotes Uvb-Induced Apoptosis In Skin, Lindsey Susie Shim

Theses and Dissertations

T-cell protein tyrosine phosphatase (TC-PTP) is a non-receptor PTP that has been shown to have various roles in signaling pathways. Our previous studies have demonstrated that the TC-PTP deficiency in the epidermis can exacerbate hyperplastic response by inducing epidermal cell proliferation in response to tumor promoter TPA or UVB exposure. This implies that TC-PTP plays a tumor-suppressive role in the epidermis and provides protection against UVB radiation, which is well known to contribute to skin cancer development. The p38 mitogen-activated protein kinase (MAPK) is one of serine/threonine kinases that are activated through environmental stress including UVB. It has been demonstrated …

A Nursery Study Of The Interplay Between Soil Texture, Rooting Space, And Nurse Plants On Seedling Growth And Root Development In Woody Species Of Tamaulipan Thornscrub, Lance I. Santos

Theses and Dissertations

Reforestation attempts in semi-arid regions are plagued with seedling mortality. Blanket greenhouse practices may not be optimal, in addition, commensalism interactions between nurse plants are considered. To better understand the effects of soil texture and nurse plant interactions on growth and drought tolerance, two experiments were developed. For soil texture, Border palo verde seedlings were grown in different-sized plant bands with three soil types. To observe the effects of nurse plants, an experiment using guayacan, was developed using Texas Ebony, as a leguminous nurse plant. Plants were also exposed to a drought experiment. The results suggest that some soil textures …

Quantifying Greenhouse Gas Exchange And Ecosystem Services In Ecosystems Managed For Nature-Based Solutions Using Eddy Covariance, Remote Sensing, And Models, Lucas Clay

All Dissertations

As global temperatures rise due to climate change, nature-based solutions are increasingly being used to restore nature and to remove carbon from the atmosphere. Nature-based solutions draw on methodologies such as ecological forestry and improved forest management to reduce emissions and maximize resilience. These protocols are still in their infancy and additional measurements and case studies are important to optimizing these protocols. Herein this dissertation we describe multiple measurement methodologies for both greenhouse gas (GHG) emissions and for ecosystem services. In coastal wetland ecosystems, the need for GHG measurements is paramount to understanding the ability of these ecosystems to reduce …

Integrating Social And Ecological Insights In The Management Plan For An Emerging Conservation Landscape: Kanha-Pench Corridor, Hrishita Negi

All Dissertations

Since its inception in 1973, Project Tiger, which laid the groundwork for India's tiger conservation program, has grown from 9 to 53 intensively monitored and managed tiger reserves. With an increase in tigers' permanent occupancy outside of designated tiger reserves, one of the many challenges confronting India's tiger conservation has been managing its success, with a particular emphasis on this spillover population that ranges extensively in human-dominated, multi-use forest landscapes. This research illustrates the application of a participatory modeling approach to integrate human dimensions with ecological knowledge about tigers in a previously identified forest corridor in Central India. Key dimensions …

Cross-Interactions Of Amyloid Proteins: Implications For Aggregation Dynamics And Disease Pathology, Zhiyuan Song

All Dissertations

Amyloid aggregation is a pervasive form of protein misfolding implicated in the pathology of several prominent human diseases, including Alzheimer’s disease (AD), Parkinson’s disease (PD), and type 2 diabetes (T2D). These diseases are marked by the accumulation of amyloid fibrils and toxic oligomers, which are highly dynamic and heterogeneous, posing challenges for understanding their roles in disease progression. Despite significant advances in the diagnosis and management of these conditions, no definitive cure is available. We investigate amyloid aggregation and cross-seeding, with a focus on amyloid-beta (Aβ), tau, alpha-synuclein (αS), and human islet amyloid polypeptide (IAPP) through discrete molecular dynamics (DMD) …

Receipt Of Home Health And 30-Day Outcomes Among Veterans With Dementia Following Inpatient Surgery, Heather Davila, Yubo Gao, Katherine E Hadlandsmyth, Andrea L Strayer, Michael A Jacobs, Paula K Shireman, Susanne Schmidt, Leslie R M Hausmann, Carly A Jacobs, Daniel E Hall, Mary Vaughan Sarrazin

Faculty, Staff and Student Publications

No abstract provided.

Radiation-Induced Macrovessel/Microvessel Disease, Jun-Ichi Abe, Bryan G Allen, Andreas M Beyer, David Lewandowski, Kranti A Mapuskar, Michelle R Tamplin

Faculty, Staff and Student Publications

Radiation therapy (RT) is a cornerstone in cancer treatment (used in 50% of cases), yet challenges persist because damage to normal tissue through direct impact of radiation or bystander effects is inevitable. Injury of macrovessels by RT manifests as obstructive disease, which is akin to atherosclerotic disease. Historically observed in coronary arteries of patients treated for breast cancer and lymphoma, it also affects patients receiving contemporary therapy for lung and chest cancers. Moreover, radiation at various sites can lead to peripheral vascular disease. An aspect of radiation-induced injury that has received little attention is microvascular injury, which typically results from …

Genetic Ablation Of The Isoform Γ Of Pi3k Decreases Antidepressant Efficacy Of Ketamine In Male Mice, Gabriela N Vaz, Flávia C Turcato, Isabel A V Lima, Franciele F Scarante, Melissa R Araújo, Livia C M Rodrigues, Jaime E C Hallak, Jose A Crippa, Antonio L Teixeira, Antonio C P De Oliveira, Alline Cristina Campos

Faculty, Staff and Student Publications

About one-third of major depressive disorder (MDD) patients demonstrate unresponsiveness to classic antidepressants, and even the clinical efficacy of fast-acting drugs such as ketamine varies significantly among patients with treatment-resistant depression. Nevertheless, the lack of suitable animal models that mimic a possible ketamine-resistant phenotype challenges the understanding of resistance to drug treatment. In this study, we showed that PI3Kγ knock-out (KO) mice do not respond to classical doses of ketamine and classical antidepressants. PI3Kγ KO mice were unresponsive to both the rapid and sustained antidepressant-like effects of a single dose of ketamine in the forced swimming test. Additionally, they were …

Keratinocyte Carcinomas In Survivors Of Childhood Cancer: A Report From The Childhood Cancer Survivor Study, Christina Boull, Yan Chen, Cindy Im, Alan Geller, Yadav Sapkota, Rebecca Howell, Miriam Conces, Louis S Constine, Leslie Robison, Yutaka Yasui, Gregory T Armstrong, Joseph P Neglia, Lucie M Turcotte

Faculty, Staff and Student Publications

Background: Childhood cancer survivors (CCS) are at increased risk for keratinocyte carcinomas (KC) however, the long-term incidence of single and multiple KC is not well established.

Objective: Identify risk factors and quantify KC cumulative incidence and multiple-incidence burden in CCS.

Methods: KC were identified among Childhood Cancer Survivor Study participants, a cohort of 5-year cancer survivors diagnosed < 21 years of age between 1970 and 1999 in North America. Cumulative incidence was estimated and multivariable models assessed relative rates of KC associated with survivor and treatment characteristics.

Results: Among 25,658 participants, 1446 developed 5363 KC (93.5% basal cell carcinoma, 6.7% squamous cell carcinoma; mean age 37.0 years (range 7.3-67.4), mean latency 25.7 years; 95.3% White and 88.4% with radiotherapy). Mean lesion count was 3.7 with 26.1% experiencing ≥4. Radiotherapy imparted …

Artificial Intelligence Uncertainty Quantification In Radiotherapy Applications – A Scoping Review, Kareem A Wahid, Zaphanlene Y Kaffey, David P Farris, Laia Humbert-Vidan, Amy C Moreno, Jintao Ren, Tucker J Netherton, Stine Korreman, Guha Balakrishnan, Clifton D Fuller, David Fuentes, Michael J Dohopolski

Faculty, Staff and Student Publications

Background/purpose: The use of artificial intelligence (AI) in radiotherapy (RT) is expanding rapidly. However, there exists a notable lack of clinician trust in AI models, underscoring the need for effective uncertainty quantification (UQ) methods. The purpose of this study was to scope existing literature related to UQ in RT, identify areas of improvement, and determine future directions.

Methods: We followed the PRISMA-ScR scoping review reporting guidelines. We utilized the population (human cancer patients), concept (utilization of AI UQ), context (radiotherapy applications) framework to structure our search and screening process. We conducted a systematic search spanning seven databases, supplemented by manual …

High Activity Of The New Myeloablative Regimen Of Gemcitabine/Clofarabine/Busulfan For Allogeneic Transplant For Aggressive Lymphomas, Jeremy Ramdial, Ruitao Lin, Peter F Thall, Benigno C Valdez, Chitra Hosing, Uday Popat, Amin Alousi, Melissa Barnett, Alison Gulbis, Terri Lynn Shigle, Elizabeth J Shpall, Borje S Andersson, Yago Nieto

Faculty, Staff and Student Publications

Refractory aggressive lymphomas can be treated with allo-SCT, pursuing a graft-vs-lymphoma effect. While reduced intensity conditioning is safe, tumors often progress rapidly, indicating the need for more active conditioning regimens. The preclinical synergy we saw between gemcitabine (Gem), clofarabine (Clo) and busulfan (Bu) against lymphoma cell lines led us to study Gem/Clo/Bu clinically. Eligibility: age 12-65, refractory aggressive B-NHL, T-NHL or Hodgkin, with a matched donor. Infusional Gem was dose-escalated on days (d) -6 and -4 (475-975 mg/m2/day), followed by Clo (40 mg/m2/day) and Bu (target AUC, 4000 μMol min/day) (d -6 to -3). CD20+ tumors received rituximab. GVHD prophylaxis …