Life Sciences Commons^™

Investigating The Acute Physiological Effects Of Effluent Crystal Violet On The Freshwater Bioindicator Daphnia Pulex, Hoang K. Ho

2024 Fall Honors Capstone Projects - Archive

This study examines the effects of acute exposure to crystal violet (CV) on the freshwater zooplankton Daphnia pulex in response to growing concerns over synthetic dyes from industrial runoff in aquatic ecosystems. The primary objective is to assess CV's impact on D. pulex's heart rate, mobility, morphology, and lethality via a series of acute exposure tests. 48-h acute toxicity (0-2000 μg/L), 48-h immobilization (0-180 μg/L), and 9-h heart rate (0-2,000 μg/L) tests were conducted using third-generation daphnids, followed by stereomicroscopic examination and statistical analysis. Results showed increasing mortality and decreasing mobility and heart rate with increasing CV concentration; the …

The Role Of Eor-1/Plzf In Promoting Soma Compartment Specific Death In A Non-Canonical Cell Death Program In C. Elegans, Nathan Rather

2024 Fall Honors Capstone Projects - Archive

Programmed cell death (PCD) is an essential evolutionarily- conserved and genetically encoded program for normal development and homeostasis. A previous study identified a CED-3 dependent but mechanistically non-apoptotic and highly stereotyped elimination program called Compartmentalized Cell Elimination (CCE) in the C. elegans tail-spike epithelial cell (TSC). Here the transcription factor EOR-1/PLZF is found to promote CCE. The loss of which results in a persisting non-engulfed soma with enlarged nuclei in the first larval stage. This study finds that EOR-1 and its established partners function downstream of CED-3 Caspase protease activity, a role distinct from other PCD contexts where EOR-1 has …

Insights Into Cerebrovascular Health: Comparative Study Of The Common Carotid And Middle Cerebral Artery, Emma Lou

2024 Fall Honors Capstone Projects - Archive

The carotid artery, responsible for blood flow to the brain, and the middle cerebral artery (MCA), which supplies the cerebrum, are both susceptible to atherosclerosis, a condition that impairs blood flow and can lead to cognitive decline and ischemic strokes. This study investigates whether blood flow and vasodilation responses in the MCA during a hypercapnic challenge are comparable to those in the common carotid artery, which supplies the entire brain. This project aims to address limitations in current assessment methods, such as transcranial and duplex Doppler ultrasonography, to ascertain whether these two arteries exhibit consistent vasodilation patterns under hypercapnic conditions. …

Analyzing Head Morphology Trends With Increasing Population Density In A Jamaican Lizard Species, Lillian Durand

2024 Fall Honors Capstone Projects - Archive

Anolis lizards are model organisms for studying natural selection and evolutionary processes. One trait that is hypothesized to be advantageous for males is head size, as larger heads lead to stronger bite forces and increased likelihood in outcompeting other males for mates. To better understand how linked head size and intraspecific competition are, the head morphology of male A. lineatopus was analyzed among Jamaican populations with varying levels of population density. Population densities were determined using standardized plot surveys and mark resight models. Head dimensions and body condition for individual lizards were measured at each site. My study found that …

Mapping The Spread: Analyzing The Invastion Of Potamogeton Crispus In The Provo River Delta (2023-2024), Chloe Francis

Undergraduate Honors Capstone Projects

This capstone project analyzed the spread and ecological impacts of curly-leaf pondweed (Potamogeton crispus) in the Provo River Delta, contributing to the broader Provo River Delta Restoration Project. Field surveys, R programming for data analysis, and spatial mapping through ArcGIS Online were used to create a StoryMap that visualized the presence and absence of curly-leaf pondweed in the delta. A literature review was conducted to gather relevant scientific information on curly-leaf pondweed, which was then synthesized into a fact sheet focused on practical control methods. This fact sheet was presented to managers at the Great Salt Lake Managers …

Quantitative Analysis Of Protective Effect Of Resveratrol Against Nanoplastic-Induced Oxidative Stress, Bryan Gustafson

All Graduate Reports and Creative Projects, Fall 2023 to Present

Nanoplastics and microplastics are a growing ecological and health concern due to their widespread prevalence. The effects of these microscopic particles in biological systems primarily relate to their potential to produce reactive oxygen species and interactive effects with other cytotoxic agents. Of particular interest in this work is the effect on mammalian intestinal cells, due to the regular unintentional ingestion of micro- and nanoplastics in the normal western diet. In this study, Caco-2 cells were cultured and exposed to polystyrene particles in vitro as a simple model of the human digestive endothelium.

Resveratrol, a naturally occurring polyphenolic compound, has been …

Assessment Of Gender Differences In Letters Of Recommendation For Physical Therapy Residency Applications, Darren Q Calley, Sunyang Fu, Marissa D Hamilton, Austin W Kalla, Christopher K Lee, Veronica A Rasmussen, John H Hollman, Hongfang Liu

Faculty, Staff and Student Publications

Introduction: Letters of recommendation (LOR) are an integral component of physical therapy residency applications. Identifying the influence of applicant and writer gender in LOR will help identify whether potential implicit gender bias exists in physical therapy residency application processes.

Review of literature: Several medical and surgical residency education programs have reported positive, neutral, or negative LOR female gender bias among applicants and writers. Little research exists on gender differences in LOR to physical therapy education programs or physical therapy residency programs.

Subjects: Seven hundred sixty-eight LOR were analyzed from 256 applications to 3 physical therapy residency programs (neurologic, orthopaedic, sports) …

Soil Organic Matter Processes From The Treetops To The Clay Particle: The Role Of Climate, Minerals, And Organic Matter Chemistry In Soil Carbon Accumulation And Stability, Jessica G. Murray

All Graduate Theses and Dissertations, Fall 2023 to Present

Soils store more carbon than plants and the atmosphere, yet release ten times more carbon dioxide (CO₂) to the atmosphere than human activities each year. Small shifts in the balance of soil carbon storage and soil carbon emissions could make climate change less severe, by acting as a sink for the carbon plants pull from the atmosphere, or make climate change worse, by releasing even more planet-warming greenhouse gases. The processes governing whether soils store or release carbon depend on tiny soil microorganisms which convert plant carbon to soil organic matter, releasing CO₂ in the process. In …

Immune Variation Across Different Contexts In Non-Model Systems, Kwanho Claudia Ki

All Graduate Theses and Dissertations, Fall 2023 to Present

The immune system is important to an animal’s survival against disease-causing agents and physical injuries. Accordingly, many resources are required to maintain and activate the immune system (such as energy). However, as these resources also need to be shared with other important systems of the body, the portion of resources that can be allotted to the immune system is variable. Thus, many different factors may alter how well the immune system operates. My dissertation sought to understand what sorts of factors can lead to changes in the immune system. Reptiles are known to have a very robust immune system. Therefore, …

Are Universities Adequately Preparing Undergraduate Students For Careers As Professional Wildlife Biologists?, Trevon D. Strange

All Graduate Theses and Dissertations, Fall 2023 to Present

The demands for diversely skilled wildlife biologists and scientific researchers in the wildlife profession are projected to increase. Increasing urbanization has affected wildlife management, and the recruitment of future wildlife professionals. Thus, state and federal supervisors in agencies that hire wildlife professionals are concerned that recent graduates of university wildlife programs lack the necessary skill-sets to address these complex contemporary wildlife management issues. My research sought to identify where the educational performance gaps may exist between university wildlife programs, wildlife agencies, and student expectations. To conduct my research, I evaluated undergraduate wildlife curricula offered by 42 accredited universities listed on …

Myosin Xva Isoforms Participate In The Mechanotransduction-Dependent Remodeling Of The Actin Cytoskeleton In Auditory Stereocilia, Ana I. López-Porras, Ava M. Kruse, Mark T. Mcclendon, A. Catalina Vélez-Ortega

Neurology Faculty Publications

Auditory hair cells form precise and sensitive staircase-like actin protrusions known as stereocilia. These specialized microvilli detect deflections induced by sound through the activation of mechano-electrical transduction (MET) channels located at their tips. At rest, a small MET channel current results in a constant calcium influx which regulates the morphology of the actin cytoskeleton in the shorter ‘transducing’ stereocilia. However, the molecular mechanisms involved in this novel type of activity-driven plasticity in the stereocilium cytoskeleton are currently unknown. Here, we tested the contribution of myosin XVA (MYO15A) isoforms, given their known roles in the regulation of stereocilia dimensions during hair …

Evaluation Of Commercially Available Sanitizers Efficacy To Control Salmonella (Sessile And Biofilm Forms) On Harvesting Bins And Picking Bags, Colton Ivers, Samhitha Chalamalasetti, Blanca-Ruiz Llacsahuanga, Faith Critzer, Manreet Bhullar, Londa Nwadike, Umut Yucel, Valentina Trinetta

Dairy Science and Food Science Faculty Publications: 2022 to Present

This study evaluated the efficacy of five commercially available sanitizers to reduce Salmonella (sessile and biofilm forms) count on experimentally inoculated materials representative of harvesting bins and picking bags in the fresh produce industry. Sessile Salmonella cells were grown onto tryptic soy agar to create a bacterial lawn, while multistrain Salmonella biofilms were grown in a Centers for Disease Control and Prevention (CDC) reactor at 22 ± 2 °C for 96 h. Samples were exposed to 500 ppm free chlorine, 500 ppm peroxyacetic acid (PAA), 75 psi steam, and 5% silver dihydrogen citrate (SDC) for 30 sec, 1, or 2 …

Unilateral Tongue Atrophy In Smad3: A Case Report, Gloria Galloway

Nursing & Health Sciences Research Journal

Background: Mothers Against Decapentaplegic Homolog 3 (SMAD3) is a disorder of the protein encoded at locus 15q22.33 and involved in the transcription of growth factor-beta (TGF) signalling. In this disorder, SMAD3 encoding is impaired, resulting in a connective tissue disorder with multisystem involvement (Bertoli-Avella et al., 2015; Dulac et al., 2019). This case is the first to describe unilateral tongue atrophy as an initial presentation of SMAD3, thereby providing an important contribution to the literature. Clinical History and Exam: A 43-year-old patient described a 1–2-week history in which he noted that half of his tongue had wasted away, and he …

First Breeding Confirmation For Red-Breasted Nuthatch (Sitta Canadensis) Along The Big Bend Reach Of The Platte River, Jacob C. Cooper, Cody Mcgregor, Letitia M. Reichart, Janet Steele

Nebraska Bird Review

The Red-breasted Nuthatch (Sitta canadensis) is a highly nomadic species of North American nuthatch prone to large movements across the entire continent (Ghalambor and Martin 2020). This behavior has resulted in both a lack of geographic variation across the continent and propensity to colonize isolated patches of suitable habitat (Ghalambor and Martin 2020). In the Bessey District of the Nebraska National Forest (NNF), Red-breasted Nuthatches were one of the first coniferous-forest bird species to colonize and begin breeding in the isolated planted stands of pine (Zimmer 1913, Bray 1994). Despite the increasing number of records for the species …

Index To Volume 92

Nebraska Bird Review

Able, K. A. 131
Adams, H. 88
Alexander, Jan 33
Allard, Savannah 33
Allen: C.R. 167; Edward 58
Allison, Betty 34
Altman, Dakota 33
Ames, Cheryl 68
Amy, Peter 68
Anderson: Ann 157; Brett 32, 34; Logan 61; Rachel 33; Will 125
Andersson, Justin 34
Ani, Groove-billed 134
Archer, Kellen 33
Arends, Jim 34
Audubon, John J. 18, 19, 20
Austgen, Jane 34
Avocet, American 58, 92, 111, 148, 169

. . .

Zach, Joel 34
Zicafoose, Kirby 32
Zimmer, J.T. 167
Zimmerman, Kelsey 63
Zoller, Lawrence 61
Zonotrichia leucophrys: leucophrys 16, 72; oriantha 16, 72

Mccook Fall Field Days, Sept. 20–22, 2024, Gary Roberts

Nebraska Bird Review

The 2024 NOU Fall Field Days were held in McCook on September 20–22, with approximately fifty-one people in attendance. The programs were held at McCook Community College Student Union. A special thanks to Boni Edwards and Jan Johnson for planning the meeting.

Field trips were led by Bill Flack—Medicine Creek Reservoir and Cambridge Park, Robin Harding—Red Willow Reservoir and Culbertson Trail, Joel Jorgenson—Swanson Reservoir and Hitchcock County, and T.J. Walker—Rock Creek Recreation Area and Dundy County. Counties included were Chase, Dundy, Frontier, Furnas, Hayes, Hitchcock, Lincoln, Perkins, and Red Willow.

A total of 139 species were observed with notable sightings …

Fall Field Report, August–November 2024, W. Ross Silcock

Nebraska Bird Review

First off, I should reiterate that records are only mentioned in this Introduction and in the text when they add to or fine-tune data included in BONO (Birds of Nebraska – Online (outdoornebraska.gov). It was my expectation that as the number of birders and in particular those reporting to eBird continue to increase, gaps in the database will be filled and my job will become simpler. However, this expectation is yet to be realized, likely due to increasing movements and activity among birds that appears to be due to increasing severity of weather events, such as heat, cold, and drought, …

Evaluation Of Biochar As A Remediation Tool For Aminopyralid Contaminated Compost, Steven James Parris

Masters Theses & Specialist Projects

Aminopyralid is a synthetic auxin herbicide which is applied to grass pastures to control broadleaf weed populations. Aminopyralid can contaminate compost through inputs derived from treated pastures such as manure and grass clippings. This possibility poses the threat of inadvertent herbicide exposure to sensitive vegetable plants through using compost contaminated with aminopyralid. Biochar can absorb pollutants and has a synergistic effect with compost when applied to the compost mixture. This study examined the relationship between biochar and aminopyralid through compost consisting of dairy manure and sawdust 1:1 mixture, aminopyralid (10μg/kg), and biochar at four treatment levels of total mass of …

The Atheroprotective Impact Of Anti-Mir-33a-5p/3p In Cultured Pro-Inflammatory Endothelial Cells, Kun Huang

All Dissertations

Atherosclerosis is the process of cholesterol buildup within arterial cells, which is considered a leading factor that causes certain cardiovascular diseases. Cholesterol accumulation within pro-inflammatory endothelial cells is thought to induce atherogenesis and accelerate atherosclerosis progression. Evidence suggests inhibiting the microRNA miR-33a in arterial cells may promote atheroprotection via increasing ABCA1 protein expression. MiR-33a is processed into two functional strands, miR-33a-5p and miR-33a-3p. The potential atheroprotective effect of inhibiting miR-33a-5p and/or miR-33a-3p precisely in pro-inflammatory endothelial cells is not fully understood, as data is scant regarding what potential atheroprotective impact ABCA1-dependent cholesterol within pro-inflammatory endothelial cells demonstrates, which occurs via …

Research Angler Program Newsletter: Recognition Of Two Decades Of The Research Angler Program, Department Of Primary Industries And Regional Development, Western Australia

Fisheries Research Reports

For over two-decades, volunteer recreational fishers have been diligently filling in angler logbooks as part of the Department of Primary Industries and Regional Development (DPIRD) Research Angler Program (RAP). The program, which began in 2004, was initially designed as a tool to enhance data collection for estuarine finfish in the West Coast Bioregion.

Throughout time, the program expanded to collect data on all species, habitat types and bioregions, generating community involvement all over the state. To date, fishers have reported data on over 19,000 fishing trips and more than 450 different fish and invertebrate (crustaceans and molluscs) species.

Fishers participating …

Evaluating The Current State Of Evolution Acceptance Instruments: A Research Coordination Network Meeting Report, M. Elizabeth Barnes, Rahmi Q. Aini, James P. Collins, Ryan D.P. Dunk, Emily A. Holt, Jamie Jensen, Joanna R. Klein, Taya Misheva, Louis S. Nadelson, Michael J. Reiss, William L. Romine, Andrew Shtulman, Amanda L. Townley, Jason R. Wiles, Yi Zheng, Sara E. Brownell

Biological Sciences Faculty Publications

Hundreds of studies have explored student evolution acceptance because evolution is a core concept of biology that many undergraduate biology students struggle to accept. However, this construct of “evolution acceptance” has been defined and measured in various ways, which has led to inconsistencies across studies and difficulties in comparing results from different studies. Many studies and essays have offered evaluations and perspectives of evolution acceptance instruments, but publications with a focus on consensus building across research teams is still needed. Further, little attention has been paid to how evolution acceptance instruments may be interpreted differently by students with varied religious …

Interspecific Selection In A Diverse Mycorrhizal Symbiosis, Megan A. Rúa, Jason D. Hoeksema

Biological Sciences Faculty Publications

Coevolution describes evolutionary change in which two or more interacting species reciprocally drive each other’s evolution, potentially resulting in trait diversification and ecological speciation. Much progress has been made in analysis of its dynamics and consequences, but relatively little is understood about how coevolution works in multispecies interactions, i.e., those with diverse suites of species on one or both sides of an interaction. Interactions among plant hosts and their mutualistic ectomycorrhizal fungi (ECM) may provide an ecologically unique arena to examine the nature of selection in multispecies interactions. Using native genotypes of Monterey pine (Pinus radiata), we performed a common …

Transcription Factor Expression Landscape In Drosophila Embryonic Cell Lines, Robert A. Drewell, Daniel Klonaros, Jacqueline Dresch

Biology

Background: Transcription factor (TF) proteins are a key component of the gene regulatory networks that control cellular fates and function. TFs bind DNA regulatory elements in a sequence-specific manner and modulate target gene expression through combinatorial interactions with each other, cofactors, and chromatin-modifying proteins. Large-scale studies over the last two decades have helped shed light on the complex network of TFs that regulate development in Drosophila melanogaster. Results: Here, we present a detailed characterization of expression of all known and predicted Drosophila TFs in two well-established embryonic cell lines, Kc167 and S2 cells. Using deep coverage RNA sequencing approaches we …

Investigating Brain Reward Mechanisms: A Comparative Study In Male And Female Rats, Valeria Garcia

Open Access Theses & Dissertations

Drug addiction is a serious public health concern. Recently, electronic cigarette use has become increasingly popular among vulnerable populations such as adolescents and women. Previous research on conventional cigarette use has shown significant sex differences in drug-seeking behavior and withdrawal symptoms, which are driven mainly by hormonal differences. However, changes in sex hormones do not completely explain the enhanced reward sensitivity reported in females. Thus, this dissertation aims to examine other alternative reasons that can explain these sex differences, such as differences in reward-seeking and neuronal reactivity. Additionally, there is a dearth of consistency across papers intending to map electrode …

Victory Gardens: Helping On The Home Front, A Curriculum Outreach Project, Kelly Hoomes

Master's Theses

The Victory Garden: Helping on the Homefront traveling trunk outreach project links past, present, and future by introducing students to the historical significance of World War II victory gardens and emphasizing the continued relevance today. Partnering with Kennesaw State University’s Museum of History and Holocaust Education (MHHE), the traveling trunk focuses on a fifth-grade audience, aligning with Georgia Standards of Excellence for Social Studies, Health, and Science. The traveling trunk designed as a “museum-in-a-box" includes primary sources, artifacts, and a practical gardening activity that fosters critical thinking and analysis. By encouraging an understanding of the past and the connections to …

The Relationship Between Chute Behavior And Morbidity In Cattle, Andrea Rankin

Animal Science Undergraduate Honors Theses

Most cattle see a handling chute at least once in their lives. Handling plays a huge role in cattle management, whether it’s for transportation, auction sales, deworming, medical treatments, or AI purposes. Morbidity related to Bovine Respiratory Disease (BRD) is one of the biggest causes of economic loss for ranchers and can be related to handling stress. Auction-derived calves (ADC; n=92) were brought to the University of Arkansas Savoy Research Facility for processing and monitoring for behavioral cues and disease. The objective of this study was to find a relationship between the calves’ behavior in the chute and morbidity. The …

The Diverse Effects Of Continual Organic Inputs On Soil Biogeochemical Properties And Microbial Communities In Sandy Ultisols Under Organic Production, Zhine Wang

All Dissertations

The objectives of this study were to understand how continual organic amendment inputs improve Nitrogen (N) cycling, microbial diversity and functional traits. Fully crossed factorial experiments were designed to test four cover crop treatments (cereal rye, hairy vetch, mixture of the rye and vetch, and no cover crop fallow) and two levels of manure compost application (with manure application and blank control) in organic vegetable production from 2020 to 2023. Cover crop biomass, soil chemical and biological properties were analyzed for the distribution and availability of soil labile N (Chapter 2), the abundance of N-cycling genes (Chapter 3), the structure …

Exploring Mechanisms Of Chemical Resistance In Xanthomonas Arboricola Pv. Pruni And Strategies To Mitigate Resistance And Improve Disease Control, Milan Panth

All Dissertations

Xanthomonas arboricola pv. pruni (XAP) is a phytopathogenic bacterium that causes bacterial spot of peach. It causes spotted lesions on leaves and fruit, leading to early defoliation and unmarketable fruit, respectively. The pathogen can overwinter in twig cankers, which serve as the primary initial inoculum source for the next season’s leaf and fruitlet infection. The current disease management practice mainly relies on routine application of chemical bactericides containing copper or oxytetracycline (OTC) in the growing season. However, disease incidence can still be high despite chemical control measures in warm, rainy seasons.

Frequent applications of copper and OTC in United …

Bap1: Genotype-Phenotype Correlation, Variant Classification, And Treatment Efficacy, Elizabeth Hobbs

All Dissertations

BRCA1-associated protein 1 (BAP1) is a tumor suppressor gene located on chromosome 3p21.3 and encodes a deubiquitinase enzyme (DUB) involved in DNA repair, cell cycle and metabolism, and apoptosis. BAP1 pathogenic germline variants are primarily associated with a familial cancer syndrome, Tumor Predisposition Syndrome 1 (TPDS1), but new missense variants have been linked to a neurodevelopmental disorder known as Kury-Isidor Syndrome (KURIS). Patients with TPDS1 variants have an increased risk for developing cancers, such as uveal and cutaneous melanomas, mesothelioma, and renal cell carcinoma, at an earlier age with lower thresholds for environmental exposures, mainly UV rays and asbestos, compared …

Characterizing Mechanisms Of Dna Repair And Genome Stability, Joshua Turner

All Dissertations

Every day our cells are bombarded with DNA lesions that threaten the stability of our genome. To help maintain genomic integrity our cells evolved to have a network of enzymes dedicated to repairing DNA lesions. Upon encountering a site of DNA damage, a cell recruits enzymes that remodel the stalled replication fork by reannealing the newly synthesized DNA together known as fork regression. Loss of fork regression activity has been shown to promote replication stress resistance after induced DNA damage. In this thesis, I discuss the role F-Box Helicase 1 (FBH1) plays in fork regression and how FBH1 promotes the …