Life Sciences Commons^™

Deuterated Water Labeling In Ibrutinib-Treated Patients With Cll: Leukemia Cell Kinetics Correlate With Ighv, Zap-70, And Mrd, Ekaterina Kim, Shih-Shih Chen, Mariela Sivina, Hyunsoo Hwang, Xuelin Huang, Alessandra Ferrajoli, Nitin Jain, William G Wierda, Dominik Wodarz, Nicholas Chiorazzi, Jan A Burger

Faculty, Staff and Student Publications

Deuterated (“heavy”) water labeling in patients with chronic lymphocytic leukemia (CLL) demonstrates that IGHV unmutated and ZAP-70+ patients have higher blood and tissue CLL death rates on ibrutinib therapy, resulting in lower measurable residual disease levels with long-term ibrutinib treatment. This trial was registered at www.clinicaltrials.gov as #NCT01752426.

A Quantitative Approach To Determining The Reduction Rate Of Cassette Labeling Errors Within A Surgical Pathology Diagnostic Laboratory, Kiaya Symonné Pruitt

Doctoral Dissertations and Projects

Cassette labeling errors within a surgical pathology laboratory can affect patient safety variously, including invalid patient results. In order to diminish these errors, automated systems have been placed in surgical pathology laboratories. However, there is no current research on reducing cassette labeling errors within a surgical pathology laboratory that possesses a partial automated system without a LIS connection. Therefore, the current study aimed to determine if there is a statistically significant reduction in pathology cassette labeling errors during different phases of the barcode system (an automated system) implementation for cassette creation. A nonexperimental correlational design was used for the current …

Administering Tolfenamic Acid To Gilts And Sows Before Artificial Insemination Did Not Improve Litter Size But Tended To Reduce Post-Service Serum Prostaglandin F2Α Levels, Pachara Pearodwong, Nithitad Jiebna, Padet Tummaruk

The Thai Journal of Veterinary Medicine

This study examined the impact of intramuscular administration of tolfenamic acid 10 min before artificial insemination (AI) on serum PGF2α levels and reproductive performance in gilts and sows. In Experiment I, 20 gilts were divided into two groups: control (n = 10) and treatment (n = 10). Serum samples were collected 30 min before and after insemination to measure PGF2α levels. In Experiment II, 175 gilts and sows were divided into control (25 gilts and 56 sows) and treatment (16 gilts and 78 sows) groups. In both experiments, the treatment group received an intramuscular injection of 2 mg/kg tolfenamic acid …

Star-Studded Stingers: Fluorescent Sensilla On The Scorpion Aculeus (Arachnida: Scorpiones), Graeme Lowe

Euscorpius

The aculeus of the scorpion telson was studied by UV fluorescence microscopy. Numerous brightly fluorescent punctae were observed on the non-fluorescent distal aculeus, including the tip region. The punctae were identified as aculear sensilla coeloconica (ASC), and were connected to fluorescent canals running through the cuticle. ASC were present in both sexes, and in adults and immatures as early as the second juvenile instar, but were absent from the first instar. The distal aculeus was found to be encased in a thick, non-fluorescent dark exocuticle (DX), covering an underlying thin, fluorescent hyaline exocuticle (HX). Fluorescent ASC were recorded from 183 …

Quality And Sensory Characteristics Of Selected Freeze-Dried Foods After 50 Years Storage, Prithiviraj Jegannathan

Theses and Dissertations

Freeze-drying is a dehydration process that uses low temperature and pressure to remove moisture from food. With an annual market value of over $35 billion in 2023, freeze-dried foods are widely used in various situations requiring long-term storage such as military rations, emergency food supplies, and space exploration. Freeze-dried foods are becoming more popular for their quality, light weight, easy storage at room temperature, and quick rehydration. Moreover, many freeze-dried food manufacturers claim that their freeze-dried food products can last for 25 years or more. However, there are currently no published studies supporting such claims. Chemical, physical, and sensory properties …

Hyperglycemia Causes Differential Change In Macrophage Population In The Lacrimal Gland, Conjunctiva And Cornea, Saleh Alfuraih, Amy Tran, Lois Kim, Rais Ansari, Ajay Sharma

Pharmacy Faculty Articles and Research

Background: Due to its location, the ocular surface is exposed to environmental microbes. Innate immune cells including macrophages are first line defense against infections. In vitro exposure to high glucose as well as diabetes-associated hyperglycemia has been shown to affect innate immune cell function and population. The present study was designed to examine the effect of diabetes-associated hyperglycemia on the lacrimal gland, conjunctiva and cornea macrophage population, phenotypic changes and cytokines/chemokines.

Methods: Mouse model of Streptozotocin-induced diabetes was used to induce hyperglycemia. Immunostaining for CD11b and F4/80 was performed to stain macrophages in whole mount cornea, conjunctiva and 50 µm …

Capacity-Weighted Figures-Of-Merit For Battery Transport Metrics, Cj Sturgill, Christopher Sutton, Julian Schwenzel, Morgan Stefik

Faculty Publications

New battery materials with improved transport are needed. Typical measurements yield widely varying voltage-dependent diffusivities and reporting practices are diverse. Some materials (e.g. first-order phase change) have most redox occur at a specific voltage and may be sufficiently represented by singular transport metrics. Many rapid intercalation materials, however, exhibit second-order phase transitions with redox over a broad voltage range. How should such cases be compared? The use of capacity-weighted average values is suggested where voltage-dependent metrics are consolidated into representative descriptors as figures-of-merit. Examples are elaborated where differential capacity (dQ/dV) is used to derive a …

Tree Rings Reveal Mixtures Of Aspen And Spruce Exhibit Greater Drought Resilience In A Planted Field Experiment, Jéssica Chaves Cardoso, Linhao Wu, Marcel Schneider, Charles A. Nock

Aspen Bibliography

Boreal forests provide a wealth of ecosystem services, many of which are linked to forest productivity. Climate change is expected to increase the frequency, duration, and severity of drought, and undermine the productivity of western boreal forests. Favouring species mixtures has emerged as a potential strategy to increase forest resilience to drought. To test the hypothesis that mixtures of trembling aspen and white spruce were more productive than pure stands because they were more resistant and resilient to drought, we analyzed community and species responses to drought utilizing measurements of annual growth obtained from stem disc samples from a planted …

Nexus Between Rural Agricultural Land Ownership And Agricultural Credits Access: A Case Of Tanzania, Gelas Rubakula

Journal of Humanities and Social Sciences

Agricultural investment in low- and middle-income countries faces credit access challenges. Several financial institutions, particularly banks, normally impose some conditions where the possession of a land title deed is required to access agricultural credits. This paper examines whether, and to what extent, rural agricultural land ownership influences agricultural credit access for rural farmers in Tanzania. The study focuses only on rural farmers and tries to find out whether these smallholder farmers can use their land, which is largely unformalized, as a collateral for credit access. The study survey was cross-sectional, using quantitative data collected in December 2017 to March 2018 …

Social Learning And Cultural Enrichment For Fish Welfare, Chiawen Chiang, Becca Franks

Animal Sentience

This commentary examines how understanding animal culture—shared behaviors and knowledge transmitted through social learning—plays a role in advancing welfare practices for captive fishes. Despite extensive evidence of fishes' complex sociocultural capacities, their psycho-social needs remain underestimated and unmet in zoos and aquaria. We explore what considering fish culture might mean for welfare practices: diverse social learning opportunities, cultural development, and thoughtful group management. More knowledge about wild fishes' lives will help bridge the gap between their full, complex lives and current captive welfare practices.

Sensitivity Of Difficult-To-Control Palmer Amaranth Accessions To Fluridone And Diflufenican, Maria Carolina Souza, Matthew Coleman Woolard, Jason K. Norsworthy, Tristen H. Avent, Tristen H. Avent, Tom Barber, Rodrigo Botelho

Crop, Soil and Environmental Sciences Faculty Publications and Presentations

Palmer amaranth (Amaranthus palmeri S. Watson) has evolved resistance to herbicides targeting nine sites of action. Phytoene desaturase-inhibiting herbicides remain among the few sites of action that are still effective in controlling Palmer amaranth. Greenhouse experiments were conducted to evaluate the effectiveness of fluridone and diflufenican in controlling difficult-to-control Palmer amaranth accessions collected in Arkansas from 2016 to 2022, to quantify the response of three accessions that exhibited low sensitivity to both herbicides, and to evaluate the effectiveness of photosystem II inhibitors alone and with fluridone or diflufenican on three accessions. At 14 days after a preemergence application, Palmer …

Technology Development Of High-Resolution Single-Cell Lineage Tracing For Cell Reprogramming Systems, Sadie Vanhorn

Arts & Sciences Graduate Student Theses and Dissertations

A critical element of cell identity is the cell that created the observed cell. This parent cell dictates many aspects of its identity, whether measured by function, phenotype, or specific assays. The cell’s lineage impacts the ability of the cell to differentiate into final cell types in development or successfully reprogram in direct cell reprogramming. Here, we evolve molecular barcoding techniques to increase the resolution of lineage recording at single-cell resolution. Using single-cell RNA-sequencing as the readout for this measurement, polyadenylated reporter gene transcripts with engineered 3’ untranslated regions were designed to initially label and then record lineage information. While …

Multi-Omic Analysis Of Phototrophic Extracellular Electron Uptake In Novel Rhodovulum Spp. Isolated From A Marine Estuary, Emily J. Davenport

Arts & Sciences Graduate Student Theses and Dissertations

Microbial metabolisms are incredibly versatile and dynamic, allowing adaptive strategies for growth in even the most limiting environments. For an ever-increasing number of microbes, this includes utilizing soluble or insoluble inorganic mineral species as electron donors, driving geochemical cycles and microbial metabolisms. This metabolic function of transporting electrons derived from the extracellular environment across cell membranes is called extracellular electron uptake (EEU). Within (photo)autotrophs, (p)EEU also sustains CO2 fixation to cell biomass, implicating a potentially fundamental role of microbial carbon cycling in aquatic and soil ecosystems. A model metabolic scheme of pEEU occurring in natural environments is photoferrotrophy, which pairs …

Agot: Agricultural Guide For Occupational Therapy Practitioners, Kara Smallegan, Meghan Skidmore Otd, Otr, Cns, Angela Labrie Blackwell Phd, Otr

Fall 2024 OTD Capstone Symposium

As agriculture is a hazardous occupation, this project discusses agricultural workers with health conditions/disabilities, experiencing disruption to occupational performance and participation, increasing risk of secondary injuries, and impacting overall well-being and how occupational therapy practitioners can improve these factors through the development of a guidebook. The guide covers population insights, risk factors, assessment guide, conditions, and resources to aid occupational practitioners when assisting the agricultural worker population.

Using Bivalves As An Indicator For Fecal Bacteria, Kamala Chuss

Honors Scholar Theses

This project focuses on the integration of fecal bacteria from warm-blooded animals into bivalve tissue, and the utilization of bivalves as an indicator for fecal bacteria in waterways. Bivalves may give a more representative long-term average of fecal bacteria in water as compared to direct water testing. The primary objective is to create a standard operating procedure to use bacterial testing of bivalves as a proxy for bacteria concentration in the water. This method can be used in places where bivalves do not already exist by adding them to a body of water, or by collecting bivalves that already exist …

Achieving Inter- And Transdisciplinarity In Ecohealth: Insights From A Rodent-Borne Disease Project In A Polycrisis Era, Isabelle Arpin, Clémence Massart, Vincent Bourret, Guillaume Castel, Valeria Carolina Colombo, Jana Eccard, Jasmin Firozpoor, Maciej Grzybek, Heikki A. Henttonen, Herwig Leirs, Andrew Mcmanus, Ben Roche, Tarja Sironen, Vincent Sluydts, Peter Stuart, Annetta Zintl, Nathalie Charbonnel

Publications

Introduction: Inter- and transdisciplinary research (ITDR) is increasingly promoted to address “wicked problems”, particularly in health sectors adopting approaches like Ecohealth. Our Ecohealth-inspired project on rodent-borne diseases, initiated just before the COVID-19 pandemic, provided an opportunity to evaluate ITDR implementation.

Methods: We employed a recently developed semi-quantitative evaluation method to measure our project’s success in achieving ITDR and analyzed factors influencing this achievement.

Results: The project showed strengths in system description, team task allocation, and data sharing, but had lower scores in engaging societal actors throughout the project cycle.

Discussion: We identified the underexplored influence of problem wickedness as a …

Comparison Of Vendor-Pretrained And Custom-Trained Deep Learning Segmentation Models For Head-And-Neck, Breast, And Prostate Cancers, Xinru Chen, Yao Zhao, Hana Baroudi, Mohammad D El Basha, Aji Daniel, Skylar S Gay, Cenji Yu, He Wang, Jack Phan, Seungtaek L Choi, Chelain R Goodman, Xiaodong Zhang, Joshua S Niedzielski, Sanjay S Shete, Laurence E Court, Zhongxing Liao, Fredrik Löfman, Peter A Balter, Jinzhong Yang

Faculty, Staff and Student Publications

Background/objectives: We assessed the influence of local patients and clinical characteristics on the performance of commercial deep learning (DL) segmentation models for head-and-neck (HN), breast, and prostate cancers.

Methods: Clinical computed tomography (CT) scans and clinically approved contours of 210 patients (53 HN, 49 left breast, 55 right breast, and 53 prostate cancer) were used to train and validate segmentation models integrated within a vendor-supplied DL training toolkit and to assess the performance of both vendor-pretrained and custom-trained models. Four custom models (HN, left breast, right breast, and prostate) were trained and validated with 30 (training)/5 (validation) HN, 34/5 left …

Characterization Of An Inorganic Powder-Based Scintillation Detector Under A Uhdr Electron Beam, Daline Tho, Sam Beddar

Faculty, Staff and Student Publications

Background: Ultra-high dose rate (UHDR) radiation therapy needs a reliable dosimetry solution and scintillation detectors are promising candidates. In this study, we characterized an inorganic powder-based scintillation detector under a 9 MeV UHDR electron beam.

Methods: A mixture of ZnS:Ag powder and optic glue was coupled to an 8 m Eska GH-4001-P polymethyl methacrylate (PMMA) optical fiber. We evaluated the dependence of the detector on dose per pulse (DPP), pulse repetition frequency (PRF), and pulse width (PW). Additionally, we determined the stability and the reproducibility of the detector.

Results: The signal ratio between the PMMA clear optical fiber and the …

Functional Properties Of Corticothalamic Circuits Targeting Paraventricular Thalamic Neurons, Guillermo Aquino-Miranda, Dounya Jalloul, Xu O Zhang, Sa Li, Gilbert J Kirouac, Michael Beierlein, Fabricio H Do Monte

Faculty, Staff and Student Publications

Corticothalamic projections to sensorimotor thalamic nuclei show modest firing rates and serve to modulate the activity of thalamic relay neurons. By contrast, here we find that high-order corticothalamic projections from the prelimbic (PL) cortex to the anterior paraventricular thalamic nucleus (aPVT) maintain high-frequency activity and evoke strong synaptic excitation of aPVT neurons in rats. In a significant fraction of aPVT cells, such high-frequency excitation of PL-aPVT projections leads to a rapid decay of action potential amplitudes, followed by a depolarization block (DB) that strongly limits aPVT maximum firing rates, thereby regulating both defensive and appetitive behaviors in a frequency-dependent manner. …

Utility Of Colposcopy For The Screening And Management Of Cervical Cancer In Africa: A Cross-Sectional Analysis Of Providers’ Training And Practices, Joël Fokom Domgue, Issimouha Dille, Freddy Gnangnon, Sharon Kapambwe, Celine Bouchard, Nomonde Mbatani, Elodie Gauroy, Nathalie Ledaga Ambounda, Robert Yu, Fatoumata Sidibe, Joseph Kamgno, Bangaly Traore, Pierre-Marie Tebeu, Gregory Halle-Ekane, Mohenou Isidore Diomande, Jean-Marie Dangou, Fabrice Lecuru, Isaac Adewole, Marie Plante, Partha Basu, Sanjay Shete

Faculty, Staff and Student Publications

Introduction: Cervical cancer is a public health issue in Africa with devastating socioeconomic consequences due to the lack of organized screening programs. The success of screening programs depends on the appropriate investigation and management of women who test positive for screening. Colposcopic assessment following positive screening results is a noteworthy issue in Africa. This study aimed to assess the utilization of colposcopy by providers in the region.

Methods: A cross-sectional study was conducted in 2021-2022 among healthcare providers involved in cervical cancer prevention activities in Africa. They were invited to report prior colposcopy training, whether they performed colposcopy and the …

Characterization Of An Inorganic Powder-Based Scintillation Detector Under A Uhdr Electron Beam, Daline Tho, Sam Beddar

Faculty, Staff and Student Publications

(1) Background: Ultra-high dose rate (UHDR) radiation therapy needs a reliable dosimetry solution and scintillation detectors are promising candidates. In this study, we characterized an inorganic powder-based scintillation detector under a 9 MeV UHDR electron beam. (2) Methods: A mixture of ZnS:Ag powder and optic glue was coupled to an 8 m Eska GH-4001-P polymethyl methacrylate (PMMA) optical fiber. We evaluated the dependence of the detector on dose per pulse (DPP), pulse repetition frequency (PRF), and pulse width (PW). Additionally, we determined the stability and the reproducibility of the detector. (3) Results: The signal ratio between the PMMA clear optical …

Transcriptomic Clustering Of Chronic Lymphocytic Leukemia: Molecular Subtypes Based On Bruton’S Tyrosine Kinase Expression Levels, Gorkem Kismali, Ganiraju Manyam, Nitin Jain, Cristina Ivan, Betty Lamothe, Mary L Ayres, Lakesla R Iles, William G Wierda, Varsha Gandhi

Faculty, Staff and Student Publications

Historically, CLL prognostication relied on disease burden, reflected in clinical stage. Later, chromosome abnormalities and genomics suggested several CLL subtypes which were aligned with response to therapy. Gene expression profiling data identified pathways associated with CLL progression. We hypothesized that transcriptome and proteome may identify functional omics associated with CLL nosology. As a test cohort, we utilized publicly available treatment-naïve CLL transcriptomics data (n = 130) and did consensus clustering that identified BTK-expression-based clusters. The BTK-High and BTK-Low clusters were validated in public and our in-house databases (n = >550 CLL patients). To associate with functional relevance, we took samples …

Factors Affecting Sport-Related Concussion Non-Disclosure In Women's Rugby-A Multi-Country Qualitative Analysis, Lisa Ryan, Ed Daly, Katherine Hunzinger

Department of Exercise Science Faculty Papers

Background and Objectives: Collision-sport athletes, such as rugby players, are at risk of sport-related concussion (SRC). Women are known to be at higher risk of SRC and may experience more severe and chronic symptomology than men. Knowledge of the factors that affect a player’s disclosure of their concussive symptoms could help to inform strategies to improve compliance with reporting and management of head injuries. The aim of this study was to investigate the factors that may impact women rugby players’ disclosure of a concussion. Methods: Twenty-eight adult (≥18 years of age) elite and semi-elite women rugby players from the UK …

Invasion Stress Mitigates Climate Stress In A Brackish Marsh Amphipod, Lorna E. Haworth, Sarah Nancollas, Susie N. Landa, Anne E. Todgham, Richelle L. Tanner

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

1. Organisms in coastal brackish ecosystems face not only highly variable environmental conditions, but the intensity and stochasticity of these environmental conditions are anticipated to increase due to climate change. While environmental changes are often presented unilaterally as stressors, there are some anthropogenic environmental transformations, such as the introduction of habitat forming plant species, that may mitigate physiological stress imposed by warming. In Suisun Marsh in the California Delta, native plant canopies allow light and heat to penetrate to the understory yet the canopies of the introduced Phragmites australis reed block out sunlight and heat for organisms living below.
2. We set …

Aging, Fast And Slow: Gene Expression Signatures Of Physiological Age Vs. Future Lifespan In Caenorhabditis Elegans, Matthew Mosley

Arts & Sciences Graduate Student Theses and Dissertations

An outstanding question in the field of aging biology is the origin of variability in lifespans among members of the same species. Traditional wisdom dictates that differences in one individual’s genes or environment, “nature” and “nurture,” could predispose that individual to a long or short life. However, these explanations are radically incomplete. Even in a model system as homogenous as C. elegans, using an isogenic population in a lab-controlled environment, we observe a distribution in lifespans comparable to that seen in outbred human populations. The first part of this dissertation focuses on characterizing this non-genetic, non-environmental variability in the aging …

Gene Expression Regulation By Au-Rich Elements And Au-Rich Element Binding Proteins, Courtney Fay Jungers

Arts & Sciences Graduate Student Theses and Dissertations

The regulation of messenger RNA (mRNA) splicing, stability, and translation efficiency are major modes for controlling gene expression – which is essential for life. RNA-binding proteins (RBPs) and microRNAs (miRNAs) are trans-acting factors that bind to cis-elements influencing the stability and translation of the mRNA transcript. AU-rich elements (AREs) are cis-elements that are targeted by AU-rich element binding proteins (ARE-BPs). Here, I show a novel role for AREs and AU-rich element binding protein 1 (AUF1) during alternative splicing and report on interactions of RBPs and miRNAs to design a new treatment for breast cancer by interrupting these interactions in the …

A Filter-Based Model Of Visual Recognition, Olivia Rose Bockler

Arts & Sciences Graduate Student Theses and Dissertations

Visual recognition, a key perceptual process, enables primates to identify faces, food, tools, and potential threats in their environment. Cortical neurons along the ventral visual stream—spanning from early visual cortex (V1) through inferotemporal cortex (IT), with long-range projections to ventrolateral prefrontal cortex (vlPFC)—are essential for visual recognition. Traditional models propose that neuronal representations increase in complexity in each stage of this pathway, culminating in object-specific representations in higher-order cortex. However, this hypothesis remains debated, particularly as artificial visual models have countered that individual neurons along this pathway may function more as filters contributing to distributed representations of objects. Thus, this …

Distinct Effects Of Extra- And Intracellular Trehalose Supplementation In Freezing Medium On The Post-Thaw Developmental Capacity Of Feline Oocytes, Saengtawan Arayatham, Wirakan Kallayanathum, Panuwat Padungros, Theerawat Tharasanit

The Thai Journal of Veterinary Medicine

Oocyte cryopreservation is a crucial technique for preserving the genetic material of female cats, yet maintaining post-thaw oocyte developmental competence remains a significant challenge. This study investigated the impact of intra- and extracellular trehalose on the developmental competence of feline oocytes following cryopreservation. In Experiment 1, immature feline oocytes were matured in vitro (IVM) with varying concentrations (0, 1.5, and 3 mM) of intracellular trehalose hexaacetate (Tre-(OAc)6). The matured oocytes were then cryopreserved and thawed prior to in vitro fertilization and embryo culture. Experiment 2 examined the effects of extracellular trehalose supplementation at concentrations of 0, 0.125, 0.25, and 0.5 …

Programmatic Accreditation: Implementation Strategies For College Kinesiology Program Directors, Kworweinski Lafontant

Topics in Exercise Science and Kinesiology

Programmatic accreditation within Kinesiology sub-disciplines, such as physical therapy and sports medicine, have typically been required at the graduate level. Within the coming decade, major certifications for exercise physiology (ACSM-EP) and strength & conditioning (CSCS) will institute programmatic accreditation requirements at the undergraduate level. This article takes a narrative review approach at examining ways that programmatic accreditation at the undergraduate level may impact incoming undergraduate students, such as necessitating earlier specialization and limiting the ability for late-stage career changes. To mitigate these impacts, college kinesiology program directors may benefit from considering the perspectives of students as they work to meet …

Game On! A Comparative Analysis Of External Load In Division Iii Soccer Players Across Gender And Position, Bianca J. De Lucia, Daniel M. Smith, Ashima Fillbach, Mary K. Feit

Topics in Exercise Science and Kinesiology

Global positioning systems have been used increasingly in sports science due to their ability to quantify external loads of athletes during competitions and practices. Limited research has examined gender differences in external load throughout a collegiate soccer season. PURPOSE: The aim of this study was to compare game workloads across collegiate men (MSOC) and women (WSOC) soccer players. Division III MSOC and WSOC players wore GPS devices during games throughout a 15-week season. METHODS: Metrics analyzed from GPS data were: total distance (TD), sprint distance (SD), number of deceleration efforts (decel) (>-3 m/s²), number of acceleration efforts (accel) ( …