Life Sciences Commons^™

Salinity Effects On Cu(Ii) Bioremediation By Chlamydomonas Reinhardtii Microalgae, Jennifer Hughes

Honors Undergraduate Theses

Microalgae offer a sustainable approach to wastewater treatment by removing pollutants such as excess nutrients, CO₂, and heavy metals. Among various species, Chlamydomonas reinhardtii shows strong potential in removing Cu(II) ions from wastewater. In addition, salinity stress in C. reinhardtii has been linked to morphological and metabolic changes that enhance lipid and phytochemical production. While salinity stress has been studied for enhancing biofuel production, its impact on microalgal removal of heavy metals, particularly Cu(II), remains largely unexplored. Understanding how varying salinity conditions affect both algal growth and the efficiency of Cu(II) remediation could provide critical insights for improving integrated wastewater …

Characterization Of Major Foliar Fungal Species Affecting Cucurbits In South Dakota, Janani Perera Waduwarage Dona

Electronic Theses and Dissertations

Cucurbit crops comprise the largest area in vegetable production in South Dakota (USDA 2022). Information on the prevalence, distribution, and fungicide sensitivity of their fungal pathogens are unknown. This study characterized the major foliar fungal species affecting cucurbits in South Dakota through a 2-year, state-wide survey. Between 2023 and 2024, 789 leaves were recovered from pumpkin, cucumber, watermelon, squash, and bottle gourd. Alternaria spp. (70.72% of leaves) Fusarium spp. (6.84%), Podosphaera xanthii (4.18%), both Alternaria spp. and Fusarium spp. (3.42%), and both Alternaria spp. and Podosphaera xanthii (1.90%), Stagonosporopsis spp. (1.27%) and other genera of fungi (4.94%) were identified using …

Spatiotemporal Habitat Use, Sex-Specific Movement, And Site Fidelity Of Walleye In Lake Sharpe, Sd, Carter S. Dollen

Electronic Theses and Dissertations

Walleye Sander vitreus are a popular sportfish among anglers in South Dakota with large economic impacts to local communities. A popular destination for Walleye anglers is Lake Sharpe, a 25,000-ha reservoir on the Missouri River, in central South Dakota. Gill net catches in Lake Sharpe show high annual variation, prompting concern that gill net catch rates may be poorly related to population abundance. A better understanding of Walleye movement and distribution could lead to increased precision in annual abundance estimates and improved active management that increases angler success. The goals of this study were: 1) determine spatiotemporal movement patterns of …

Quantifying Avian And Vegetation Communities On Agricultural Conservation Easement Wetlands In Eastern South Dakota, Katherine Millman

Electronic Theses and Dissertations

The Prairie Pothole Region of eastern South Dakota contains numerous small depressional wetlands, colloquially known as potholes, that provide important ecosystem services, including water storage, water quality improvement, and wildlife habitat. The Agricultural Conservation Easement Program (ACEP) was established by the U.S. Department of Agriculture to protect and restore wetlands and grasslands on private land through easements such as Wetland Reserve Easements. Although ACEP requires management practices intended to maintain or improve habitat quality, few studies have evaluated their effectiveness at supporting wildlife communities and vegetation integrity. To address this gap, we evaluated wetlands at 30 ACEP sites and 20 …

Enhancing Alfalfa Persistence In Rangelands: Evaluating Morphological Traits Of Medicago Sativa Subsp. Falcata Germplasm, Abdulallah Mohammed Alomair

Electronic Theses and Dissertations

Rangelands cover nearly half of the Earth’s surface, representing about 54% (79 million km2), and occur in different types and climates worldwide. Alfalfa (Medicago sativa L.) is a major component in rangelands and covers about (320 thousand km2), representing about 0.4% of total global rangeland area. As a critical perennial legume crop, alfalfa can improve soil health by fixing approximately 336 kg/ha/year of nitrogen and sequestering up to 614 kg/ha/year of carbon, while enhancing livestock production due to its high content level of vitamins, magnesium, minerals, fiber, and protein. However, environmental stresses such as drought, freezing, and grazing limit alfalfa …

Epidemiological And Diagnostic Approaches For Soybean Cyst Nematode In South Dakota, Nabina Karki

Electronic Theses and Dissertations

Soybean cyst nematode (SCN), Heterodera glycines (HG) Ichinohe, is an endoparasitic nematode and one of the major yield-limiting pathogens of soybean (Glycine max L.) in the United States. Soybean cyst nematode alone accounts for more than $1.5 billion economic loss annually in North America. First reported in the U.S.A. in 1954 in New Hanover County, North Carolina and later in 1995 in Union County, South Dakota. Prior to this study, SCN has spread in 34 counties in the state. As a soil pathogen, which can adapt to most soil conditions under soybean production and beside soybean, it is hosted on …

Evaluation Of Two Stem Boring Insects In South Dakota: A Survey Of Wheat Stem Sawfly And Determining The Impact Of Planting Date On Dectes Stem Borer Infestations In Sunflowers, Khuma Kumari Bhusal

Electronic Theses and Dissertations

Wheat, Triticum aestivum L., and sunflower, Helianthus annuus L., are major crops in South Dakota that are threatened by stem-boring insect pests capable of reducing yield and harvestability. In wheat, the wheat stem sawfly, Cephus cinctus Norton, has long been recognized as a destructive pest in the Northern Great Plains, yet its actual distribution within South Dakota remained uncertain. To determine its presence, a two-year survey was conducted across the state from 2023–2024, during which one to four wheat fields per county were sampled using standardized sweep net transects. Wheat stem sawflies were collected only from Harding and Perkins Counties …

Soybean Meal And Soybean Hulls Inclusion In Swine Diets And The Effects On Performance, Carcass Characteristics, Nutrient Digestibility, Manure Nutrient Output, And Economic Impact, Joseph G. Halbur

Electronic Theses and Dissertations

The increasing demand for soybean production in the Midwest, driven by new soybean crushing plants, may reduce the cost of soybean meal (SBM), making it a more accessible and economically viable feed ingredient in swine diets. Replacing dried distillers grains with solubles (DDGS) with SBM and soy hulls at different inclusion rates may influence growth performance, carcass traits, nutrient digestibility, manure nutrient output, pelleting energy use, and economic returns in wean-to-finish pigs. Two experiments were conducted to evaluate these effects. In the first, 566 pigs (PIC × Terminal Duroc, 8.45 and 7.25 kg, 6 weeks of age) were housed in …

Management Strategies To Improve Gastrointestinal Health And Productivity Of Native And Dairy-Derived Steers Fed In Confinement, Becca B. Grimes Francis

Electronic Theses and Dissertations

The objective of this dissertation was to evaluate strategies to mitigate metabolic disorders in confinement fed steers. The first study was conducted on yearling beef steers to investigate the effect of a rumen buffer, calcium magnesium carbonate (CMC), when fed with or without tylosin phosphate (TYL), on growth performance, rumination activity and liver abscess prevalence. Supplementation of a rumen buffer was not an effective alternative to a feed-grade antimicrobial for the control of liver abscesses in beef steers. Supplementation of CMC reduced DMI, thus negatively influencing BW and ADG. No associative effects of CMC and TYL were observed for any …

Discovery Of Photosynthetic Oxic N2-Fixation In Cyanobacteria Using Wet Lab And Machine Learning Approaches, James A. Young Iii

Electronic Theses and Dissertations

No abstract provided.

In The Footsteps Of Moose: Using Non-Invasive Techniques To Identify Cause-Specific Calf Mortalities And Evaluate Moose (Alces Alces) Movements Pertaining To Mineral Lick Locations On Ancestral Lands Of The Grand Portage Ojibwe, Anna B. Weesies

Electronic Theses and Dissertations

Following a sharp moose (Alces alces; mooz, Anishinaabemowin, Ojibwe language) population decline in northeastern Minnesota during the early 2000s, the Grand Portage Band of Lake Superior Chippewa initiated a long-term moose collaring effort to assess survival and mortality causes. Moose are a culturally important species to the Band, with annual subsistence hunts held on the Reservation and adjacent 1854 Ceded Territory. Continued research guides culturally appropriate management decisions aimed at bolstering calf and adult survival, helping to preserve this moose in Minnesota today and for subsequent generations. Chapter 1 identifies cause-specific calf mortality rates in this multi-predator system using minimally …

Characterizing A Free-Roaming Cat Population To Predict Predation Of Threatened Species In An Island Ecosystem, Sarah Gheida

Electronic Theses and Dissertations

No abstract provided.

An Integrated Wetland Condition Index And Environmental Metric Framework For The Prairie Pothole Region, Mercedes L. Batalla

Electronic Theses and Dissertations

The USDA-NRCS Agricultural Conservation Easement Program (ACEP) assists landowners with protecting, restoring, and enhancing wetlands. Evaluating wetland conservation efforts is crucial for informing future management decisions. However, assessment frameworks vary across regions and among stakeholders. The Prairie Pothole Region is an ecologically unique landscape characterized by its abundance of wetlands that provide important ecosystem services. To evaluate wetlands enrolled in ACEP, I sampled forty-five semi-permanent wetlands in the Prairie Pothole Region from July – August 2023 and 2024. I selected and sampled twenty-six wetlands enrolled in ACEP. Nineteen additional wetlands not enrolled in ACEP were also sampled; these included nine …

The Formation And Degradation Of Carboxysomes During Heterocyst Development In Filamentous Cyanobacteria, Maxwell Anton Jakubiak

Electronic Theses and Dissertations

Photosynthetic carbon (CO2) fixation and solar-powered nitrogen (N2) fixation are foundational biochemical processes that introduce essential elements into the biosphere. While atmospheric dinitrogen gas (N2) is unusable to most organisms, diazotrophs utilize the nitrogenase enzyme to reduce N2 to ammonia. Because all known nitrogenases are highly susceptible to irreversible oxygen inactivation, N2 fixation is generally incompatible with oxygenic photosynthesis. However, several cyanobacteria are both photosynthetic and diazotrophic, balancing these opposing metabolisms by temporally or spatially separating the respective enzymes. Anabaena sp. PCC 7120 is a model multicellular cyanobacterium that grows in filaments of photosynthetic vegetative cells, which respond to nitrogen …

Isolation, Characterization & Identification Of Cultivable Field Pea Seed Bacteria In South Dakota, Diana Wairimu Njuguna

Electronic Theses and Dissertations

Pea (Pisum sativum L.) is a legume, cultivated on over 25 million acres, particularly in Canada, France, China, Russia, and the United States. Its seeds harbor microbiota including bacteria and fungi, either on the surface (epiphytes) or within seed tissues (endophytes). These microbes can be pathogenic or beneficial, enhancing seedling metabolism and protection from environmental stresses. Characterizing beneficial seed microbiota enables development of microbial inoculants for seed treatment. Seeds from 12 varieties (green or yellow seed coats) were harvested from Sturgis and Dakota Lakes in 2021. Epiphytes were extracted using sonication followed by grinding the seeds to obtain endophytes. Isolates …

Cover Cropping Strategies For Improving Weed Suppression, Yashar Askarzadeh

Electronic Theses and Dissertations

Cover crops (CCs) play an essential role in sustainable agriculture by providing multiple ecosystem services. However, adoption remains low due to concerns about economic returns, management complexity, and potential yield reductions in cash crops. Applying CCs uniformly across fields without accounting for spatial variability can result in inconsistent outcomes. A precision cover cropping system (PCCS), which incorporates site-specific management zones (MZs), offers a more targeted approach to improving performance while minimizing costs and uncertainties. This study explores the interaction between CCs and field variability through complementary field and greenhouse experiments. The field experiment demonstrates that field heterogeneity significantly influences CC …

A Multifactorial Approach To Improve Piglet Health And Survival, Aryeri Stephanie Bardales Castellanos

Electronic Theses and Dissertations

Pre-weaning mortality (PWM) remains a significant challenge in modern swine production systems, particularly in the context of hyper prolific sows. Although genetic selection has successfully increased litter sizes, these advancements have also intensified physiological stress on sows and increased the vulnerability of neonatal piglets. The majority of PWM occurs within the first 72 hours postpartum, with crushing by the sow as the leading cause of death. Additionally, iron deficiency anemia remains one of the most widespread nutritional disorders in piglets, further compromising survival, immune function, and growth. This thesis adopts a multifactorial approach to evaluate two management interventions aimed at …

Impact Of Automated Controlled Tile Drainage On Field Discharge Water, Soil Moisture, And Crop Yield In Southeastern South Dakota, Joshua Becker

Electronic Theses and Dissertations

Automated controlled tile drainage is an innovative approach to water management, using dynamic weir settings programmed to drain water out of the tile system if it encroaches into the root zone at pre-programmed depths and duration. While past studies have indicated potential yield and water quality benefits of controlled drainage, there has been very little research into the performance of automated controlled drainage. In addition to overall performance, there is a gap in knowledge of management settings to optimize yield and water quality benefits. Two experiments, one plot-scale and one modeling, were conducted to assess the impact of automated controlled …

The Effects Of Flaxseed Oil-Based Supplementation On Feeding Behavior, Performance, Follicular Dynamics, And Embryo Production In Beef Cattle, Kendal Lynn Green

Electronic Theses and Dissertations

This review seeks to provide an overview of fatty acid supplementation in cattle, opening with their structural and functional properties. Nutritional supplementation helps to regulate the precursors and substrates crucial to reproduction. This review aims to examine nutritional supplementation strategies, the role of fatty acids on follicular dynamics, and their impact on embryo production. The base of literature regarding lipid supplementation is vast, with varying results found across studies. Comparing results from prior studies remains difficult as there is a vast range of lipid sources used in beef and dairy animals. The emphasis of this review was placed on evaluating …

Mechanisms Leading To Cell Survival Through Membrane Repair In Pyroptosis, Prafulla Shrestha

Electronic Theses and Dissertations

No abstract provided.

Interactions Between Lipopolysaccharide And Capsule Retention In Klebsiella Pneumoniae And The Resultant Effects On Macrophages, Juliane Leigh Nguy

UNF Graduate Theses and Dissertations

Klebsiella pneumoniae is the third most common cause of nosocomial infections in the United States. The capsular polysaccharides (CPS) are virulence factors allowing K. pneumoniae to evade host immune systems. The current model of capsule structure indicates that CPS fibrils interact with the external O-antigen of lipopolysaccharide molecules to enhance retention to the bacterial outer membrane. These interactions may influence pathogenicity in clinically relevant strains. This study characterized alterations to LPS and CPS structure, the impact of these changes on capsule retention, membrane permeability, biofilm formation, and macrophage interactions. LPS and CPS were quantified and visualized in four strains of …

Use Of Coelomic Fluid As A Noninvasive Indicator Of Reproductive Status In Elasmobranchs, Emily Sapp

UNF Graduate Theses and Dissertations

Information on reproduction in elasmobranchs is important for the management and conservation of populations. However, the ability to obtain reliable data on elasmobranch reproduction without animal euthanasia is extremely limited. Therefore, there has been a call for nonlethal approaches for identifying reproductive status of elasmobranchs. Although useful, current techniques can be limited with respect to access to equipment and reliability of blood analyses. This study investigates whether a new noninvasive approach, the examination of coelomic fluid (CF), can provide a reliable indicator of reproductive status. CF is fluid that bathes the internal organs of the abdominal cavity, including ovaries, and …

Urban Heat Island Effects On Arthropod Populations And Communities In Jacksonville, Fl, Eleanor G. Cronin

UNF Graduate Theses and Dissertations

The urban heat island (UHI) effect makes urban areas hotter than rural areas. Urban areas, and the extreme heat that comes with them, are becoming more common worldwide. If UHIs lead to changes in arthropod community diversity, then ecosystem structure and services could be disrupted. Previous UHI studies have investigated heat impacts on arthropod ecology, but many focus on one species while studies that consider thermal diversity within a single urban area have been limited. In Jacksonville, Florida, areas that are 12°C hotter than the coolest areas are considered local UHIs. The goal of this research is to investigate how …

Précis Of The Edge Of Sentience: Risk And Precaution In Humans, Other Animals, And Ai, Jonathan Birch

Animal Sentience

We often face grave practical decisions that seem to hinge on whether a system is sentient. This family of cases includes invertebrate animals, people who are unresponsive after brain injury, fetuses, neural organoids, and now AI technologies. We must decide what to do despite ongoing disagreement about the nature of sentience. In our state of uncertainty, we should pragmatically transform the question from “Is it sentient?” to “Is it a sentience candidate, an investigation priority, or neither?”. When a system is a sentience candidate, it is negligent to fail to consider precautions. We should instead evaluate precautions for their proportionality …

Parting The Corn Rows: Nikita Khrushchev’S Visit To Iowa, 1959, Jordan Stiles

Dissertations and Theses @ UNI

On September 23, 1959, Soviet leader Nikita Khrushchev visited Roswell Garst to inspect US hybrid corn and agricultural methods. Khrushchev’s initial reforms are discussed to show why Khrushchev sought agricultural knowledge abroad. The details of Khrushchev’s and Garst’s life regarding their work in agriculture are explored up to the visit. The visit would leave an indelible mark on the USSR and Iowa. Khrushchev and his successors would use Garst’s model for hybrid corn farming as the template for Soviet farming. Garst would use his limelight to catapult his corn diplomacy into action around the world. Lastly, the legacy of Khrushchev’s …

Severe Aggression Among Zoo-Housed Coconut (Trichoglossus Haematodus) And Rainbow (Trichoglossus Moluccanus) Lorikeets: Wellbeing Considerations For Free-Flight Aviaries, Emily Simkins

UNF Graduate Theses and Dissertations

As conservation and education institutions, modern zoos balance the needs of animals with the interests of visitors through several strategies which can increase animal wellbeing and improve visitor experience. Environmental enrichment is frequently employed to increase the suitability of anthropogenic environments for zoo-housed animals, such as through walkthrough habitats and free-flight aviaries. While habitats such as these are important for visitor experience and can be enriching to animals, there are also wellbeing considerations for these spaces. Walkthrough habitats can be a potential source of fear and stress for animals, as shown by increased frequencies of vigilance, undesirable behavior, and aggression. …

Leucine Catabolism Is Associated With Longevity In Female Eastern Lubber Grasshoppers, Kerri R. Conklin

UNF Graduate Theses and Dissertations

Background & Aims: Dietary isoleucine restriction can improve metabolic health and increase lifespan. It has been hypothesized that shifts in branched-chain amino acid catabolism drives this longevity effect. Impaired branched-chain amino acid catabolism is linked to several age-related diseases. We investigated whether dietary isoleucine restriction increases leucine catabolism and associates with longevity and full reproduction.

Methods: In two experiments, adult female Eastern Lubber grasshoppers (Romalea microptera) were assigned to either a high-quality diet (Balanced ile), or diets with varying isoleucine content (Moderate ile-R and Severe ile-R). In Experiment 1, we used a low-energy (Low Lettuce) group as a positive …

Long-Term Abundance Estimates Of A Northeast Florida Estuarine Dolphin Community: Evaluating The Impacts Of The 2013–2015 Cetacean Morbillivirus Unusual Mortality Event, Allison L. Wilkins

UNF Graduate Theses and Dissertations

Tamanend's bottlenose dolphins (Tursiops erebennus) in the St. Johns River (SJR), Jacksonville, FL, are subjected to substantial anthropogenic stressors (e.g., vessel traffic, dredging, fisheries interactions) and were heavily impacted by the 2013-2015 Mid-Atlantic cetacean morbillivirus unusual mortality event (UME). High prevalence of skin lesions and frequent sightings of emaciated dolphins suggest declining health and viability of the SJR dolphin community. Sighting data from Jul. 2011 to Oct. 2019 provided the rare opportunity to investigate long-term changes in dolphin abundance surrounding the UME. This study produced the first dolphin abundance estimate within the Jacksonville Estuarine System stock since 1997, …

Fine Root And Ectomycorrhizal Functional Traits Of Quercus Rubra Populations From Across A Latitudinal Gradient, Emma L. Shedd

Dissertations, Master's Theses and Master's Reports

Forests are increasingly considered for their carbon sequestration value, but their ability to store carbon is dependent on a close balance between carbon fluxes into and out of ecosystems. Nutrient uptake of roots and associated symbiotic communities results in respiratory carbon release, which is projected to increase with warming and represents a substantial uncertainty in earth system modeling. Traits that are functionally related to nutrient uptake can be used to estimate carbon fluxes, but our understanding of the trade-offs that govern root, and especially fungal, traits is limited. Fine roots in surface soils are responsible for the bulk of plant …

Review Of Applications Of Deep Learning In Veterinary Diagnostics And Animal Health, Sam Xiao, Navneet K. Dhand, Zhiyong Wang, Kun Hu, Peter C. Thomson, John K. House, Mehar S. Khatkar

Research outputs 2022 to 2026

Deep learning (DL), a subfield of artificial intelligence (AI), involves the development of algorithms and models that simulate the problem-solving capabilities of the human mind. Sophisticated AI technology has garnered significant attention in recent years in the domain of veterinary medicine. This review provides a comprehensive overview of the research dedicated to leveraging DL for diagnostic purposes within veterinary medicine. Our systematic review approach followed PRISMA guidelines, focusing on the intersection of DL and veterinary medicine, and identified 422 relevant research articles. After exporting titles and abstracts for screening, we narrowed our selection to 39 primary research articles directly applying …