Life Sciences Commons^™

Optimizing Ethanol-Water Cosolvent Systems For Green Supercritical Carbon Dioxide Extraction Of Muscadine Grape Pomace Polyphenols, Arda Tuhanioglu, Sumanjot Kaur, Gabriel Laquete De Barros, Safoura Ahmadzadeh, Renee Threlfall, Ali Ubeyitogullari

Food Science Faculty Publications and Presentations

This study evaluated the ethanol-water modified (50%, v/v) supercritical carbon dioxide (SC-CO2) for the extraction of polyphenols from muscadine grape (Vitis rotundifolia Michgx.) pomace and compared with conventional solvent extractions (ethanol-water and HCl-methanol). The process was optimized with a central composite response surface design consisting of three levels of three independent variables: pressure (20-40 MPa), temperature (40-60 degrees C), and cosolvent concentration (5-15%) to maximize three responses: total phenolic content (TPC), total flavonoid content (TFC), and resveratrol yields. The optimal conditions were determined as 20 MPa, 60 degrees C, and 15% cosolvent concentration with TPC, TFC, and resveratrol yields of …

Local Tissue Damage Treatment Of Snake Venom, Suchitra Khunsap

The Thai Journal of Veterinary Medicine

Local tissue damage caused by snake envenomation was a medical problem that caused morbidity and mortality for more than a decade, with no defined treatment guidelines. Nowadays, antivenom is the primary treatment for snakebite, which substantially reduces systemic venom but not significantly reducing local symptoms. We compiled a list of local damage therapies, including antivenom medication, surgery and skin grafting, small molecule medicines, and alternative therapies. Although each was not approved as a standard treatment, it may be a treatment option for tissue damage caused by snake venom. However, antivenoms and most resistant compounds may not be the best strategy …

Ai-Integrated Cross-Disciplinary Liberal Arts Colloquium, Diana S. Perdue, Niloofar Gholamrezaei, Jennifer Krusinger, Shannon Hogan

Teaching Repository of AI-Infused Learning

What began organically as an AI-focused collaboration between faculty in different disciplines along with researchers in the college’s Center for Instructional Innovation, has evolved into college-wide pedagogical workshops and the development of a studio model that affects courses, programs, and paradigms for faculty scholarship.

Lessons from these collaborations inform understandings of how key features of liberal arts education can be supported through intentional AI-integration within a Cross-Disciplinary Liberal Arts Colloquium. More specifically, this colloquium offers recommendations on adapting a collaborative, interdisciplinary, and reflective studio model to in-person, online, and hybrid educational environments facilitated through a Technological Pedagogical Content Knowledge (TPACK) …

Cultural Capital Skill Development In Undergraduate Kinesiology Curriculum, Jenica Alvarez, Sabrina R. Fordham, Elizabeth M. Harrison, Blake Podsen, Jason C. Casey, Rich Pruett

Topics in Exercise Science and Kinesiology

Topics in Exercise Science and Kinesiology Volume 6: Issue 1, Article 2, 2025. Specialization within Kinesiology and related fields over the past 20 years has led to the prioritization of technical skill development. This specialization leaves limited availability of resources for the development of Cultural Capital (CC) skills in undergraduate kinesiology and kinesiology-related curricula. The purpose of this study was to examine how seven Experiential Learning Activities (ELAs) influence CC skill development in an undergraduate kinesiology practicum course. An interpretive qualitative action research design was applied to investigate five senior undergraduate kinesiology students’ experiences. The study concluded: (a) six of …

Metabolic Effects Of R-1,3-Butanediol (Ketone-Iq) During Aerobic And Anaerobic Exercise Bouts, Mathew C. Soto, Andrew J. Jakiel, Latt Mansor, Jenica D. Alvarez, Ashley M. Bruce, Blake Podsen, Sabrina R. Fordham, Parker N. Hyde

Topics in Exercise Science and Kinesiology

Topics in Exercise Science and Kinesiology Volume 6: Issue 1, Article 1, 2025. BACKGROUND: Current evidence demonstrates the efficacy of low carbohydrate/high fat ketogenic diets as it related to exercise. Exogenous ketone supplements have made it possible to ingest ketone molecules, elevating blood b-hydroxybutyrate (BHB) despite consuming carbohydrates (CHO). The purpose of this investigation was to determine both metabolic and performance effects of R-1,3-butanediol (BDO). METHODS: A randomized repeated measures placebo-controlled design was used to compare BDO and placebo (PLA). Each phase of testing utilized two primary testing days: 1) a dose exposure day designed to determine blood metabolite changes …

Evaluation Of Variable Selection Techniques On The Genetic Architecture Of Flowering Time In Maize, Felix Yeboah

Data Science and Data Mining

In this project, we investigate several variable selection procedures to give an overview of how well they perform on a genomic dataset using three different penalized regression approaches. Comparisons between different methods were performed. These methods include Ridge, lasso, and Elastic Net. We utilized 4494 observations with 7389 SNPs gene scores to predict time to male flowering (dtoa). We assessed the performance of these three models in terms of mean square error. Not surprisingly, Lasso and Elastic Net perform better than Ridge Regression. Overall, Elastic Net performed better in predicting the time of male flowering (dtoa).

A Linear Periodized Resistance Training Program Is Effective At Reducing Depressive Symptoms But Not Anxiety In Females: A Pilot, Jason Sawyer, Matthew Mcpherson, Meghan Reuter, Paul A. Cacolice

Biological and Biomedical Sciences Department Faculty Journal Articles

The purpose of this study was to determine whether a periodized resistance training program would influence self-reported depression and anxiety scores in college-aged females. Methods: Eight participants participated in a six-week periodized resistance training program. The participants completed a 3–5 repetition maximum (3–5 RM) for the sumo deadlift (SDL), bench press (BP), barbell back squat (BBS), and standing shoulder press (SSP). These data were used to estimate the 1 repetition maximum (1RM), which in turn was used to develop the periodization program. Following baseline testing, participants participated in two full-body workouts per week for six weeks. Each individual was retested …

Animal Science News (January 28, 2025), Department Of Animal Science

Animal Science Newsletters

No abstract provided.

Long-Term Spatiotemporal Analysis Of Crop Water Supply–Demand Relationship In Response To Climate Change And Vegetation Greening In Sanjiang Plain, China, Chi Xu, Wanchang Zhang, Zhenghui Fu, Hao Chen, Xia Jiang, Shuhang Wang, Bo Zhang, Zhijie Zhang

Environment and Society Faculty Publications

The Sanjiang Plain (SJP) in Northeast China, a crucial black soil region, serves as a quintessential example of a high-intensity agricultural development zone and stands as China's largest commercial grain production base. In the context of global climate change, pronounced global warming and increased vegetation greening are expected to significantly impact the agricultural water resource supply and its alignment with crop water requirements in the SJP. This study assesses how climate change and vegetation greening affect the crop water supply–demand relationship in the SJP, addressing the critical question of whether natural precipitation can sustain regional agricultural development. Using the extensively …

A Morphometric Signature To Identify Ductal Carcinoma In Situ With A Low Risk Of Progression, Marcelo Sobral-Leite, Simon P Castillo, Shiva Vonk, Hendrik A Messal, Xenia Melillo, Noomie Lam, Brandi De Bruijn, Yeman B Hagos, Myrna Van Den Bos, Joyce Sanders, Mathilde Almekinders, Lindy L Visser, Emma J Groen, Petra Kristel, Caner Ercan, Leyla Azarang, Jacco Van Rheenen, E Shelley Hwang, Yinyin Yuan, Renee Menezes, Esther H Lips, Jelle Wesseling

Faculty, Staff and Student Publications

Ductal carcinoma in situ (DCIS) may progress to ipsilateral invasive breast cancer (iIBC), but often never will. Because DCIS is treated as early breast cancer, many women with harmless DCIS face overtreatment. To identify features associated with progression, we developed an artificial intelligence-based DCIS morphometric analysis pipeline (AIDmap) on hematoxylin-eosin-stained (H&E) tissue sections. We analyzed 689 digitized H&Es of pure primary DCIS of which 226 were diagnosed with subsequent iIBC and 463 were not. The distribution of 15 duct morphological measurements was summarized in 55 morphometric variables. A ridge regression classifier with cross validation predicted 5-years-free of iIBC with an …

Optimal Size And Peg Coating Of Gold Nanoparticles For Prolonged Blood Circulation: A Statistical Analysis Of Published Data, Dmitry Nevozhay, Ronald Rauch, Zhongya Wang, Konstantin V Sokolov

Faculty, Staff and Student Publications

This study presents a statistical analysis of how gold nanoparticle (GNP) size and polyethylene glycol (PEG) coating molecular weight (MW) affect the circulation of nanoparticles in blood. We showed a non-linear relationship with interaction between GNP size and PEG MW. The findings revealed a threshold effect, and recommendations for GNP coating are discussed.

Trmt1l-Catalyzed M22g27 On Tyrosine Trna Is Required For Efficient Mrna Translation And Cell Survival Under Oxidative Stress, Sseu-Pei Hwang, Han Liao, Katherine Barondeau, Xinyi Han, Cassandra Herbert, Hunter Mcconie, Amirtha Shekar, Dimitri G Pestov, Patrick A Limbach, Jeffrey T Chang, Catherine Denicourt

Faculty, Staff and Student Publications

tRNA modifications are critical for several aspects of their functions, including decoding, folding, and stability. Using a multifaceted approach encompassing eCLIP-seq and nanopore tRNA-seq, we show that the human tRNA methyltransferase TRMT1L interacts with the component of the Rix1 ribosome biogenesis complex and binds to the 28S rRNA as well as to a subset of tRNAs. Mechanistically, we demonstrate that TRMT1L is responsible for catalyzing N2,N2-dimethylguanosine (m22G) solely at position 27 of tRNA-Tyr-GUA. Surprisingly, TRMT1L depletion also impaired the deposition of 3-(3-amino-3-carboxypropyl) uridine (acp3U) and dihydrouridine on tRNA-Tyr-GUA, Cys-GCA, and Ala-CGC. TRMT1L knockout cells have a marked decrease in tRNA-Tyr-GUA …

Erk Activation Dynamics In Maturing Oocyte Controls Embryonic Nuclear Divisions In Caenorhabditis Elegans, Han Bit Baek, Debabrata Das, Shin-Yu Chen, Hongyuan Li, Swathi Arur

Faculty, Staff and Student Publications

ERK activity oscillates between sustained activation during oocyte formation and transient inactivation during oocyte maturation, fertilization, and early embryogenesis. Consequences of ectopic ERK activity upon oocyte maturation and in early embryogenesis are unknown. We show, in Caenorhabditis elegans, that ectopic ERK activity upon oocyte maturation (metaphase I oocytes) results in embryos with abnormalities in nuclear divisions leading to embryonic death. We uncover that ERK directly phosphorylates Polo-like kinase I (PLK-1), on Serine 404, to inhibit nuclear envelope breakdown (NEBD) in early embryogenesis. The RAS/ERK/PLK-1 pathway poisons zygotic NEBD and inhibits the merging of parental genomes, underlining the importance of turning …

Overcoming Cd226-Related Immune Evasion In Acute Myeloid Leukemia With Cd38 Car-Engineered Nk Cells, Luciana Melo Garcia, Achintyan Gangadharan, Pinaki Banerjee, Ye Li, Andy G X Zeng, Hind Rafei, Paul Lin, Bijender Kumar, Sunil Acharya, May Daher, Luis Muniz-Feliciano, Gary M Deyter, Gabriel Dominguez, Jeong Min Park, Francia Reyes Silva, Ana Karen Nunez Cortes, Rafet Basar, Nadima Uprety, Mayra Shanley, Mecit Kaplan, Enli Liu, Elizabeth J Shpall, Katayoun Rezvani

Faculty, Staff and Student Publications

CD226 plays a vital role in natural killer (NK) cell cytotoxicity, interacting with its ligands CD112 and CD155 to initiate immune synapse formation, primarily through leukocyte function-associated-1 (LFA-1). Our study examined the role of CD226 in NK cell surveillance of acute myeloid leukemia (AML). NK cells in patients with AML had lower expression of CD226. CRISPR-Cas9 deletion of CD226 led to reduced LFA-1 recruitment, poor synapse formation, and decreased NK cell anti-leukemic activity. Engineering NK cells to express a chimeric antigen receptor targeting the AML antigen CD38 (CAR38) could overcome the need for CD226 to establish strong immune synapses. LFA-1 …

Humination Modification: A Green Approach To Improve The Material Properties Of Scots Pine (Pinus Sylvestris L.) Sapwood, Amir Ghavidel, Arantxa Eceiza, Xinfeng Xie, Reza Hosseinpourpia

Michigan Tech Publications

Recently, wood modification with environmentally friendly modification agents has received special attention. To this end, this study was conducted to use humin fractions, in combination with citric acid (CA) and succinic acid (SA), as reaction catalysts for the modification of Scots pine (Pinus sylvestris L.) sapwood. The effects of humination modification were evaluated by means of dimensional stability, static and dynamic mechanical properties, thermal stability, crystalline structure, and biological durability tests on modified samples and compared with the unmodified reference ones. According to the results, the dimensional stability of the huminated samples significantly increased, and this increase with the presence …

Screening Young Adult Cancer Patients For Vocational Rehabilitation Services: A Vocational Rehabilitation Readiness Screening Tool, Rhonda Robert, Clark R Andersen, Kathleen M Murphy, Sandra Medina-George, Melissa Scardaville, Sha-Ron Conley, David Farris, Michael Roth, Vinh Nguyen, Lex Frieden

Faculty, Staff and Student Publications

Purpose: We aimed to develop an online vocational rehabilitation (VR) readiness screening (VRRS) tool for young adults diagnosed with cancer. VR readiness was defined as being physically and cognitively ready to enter or return to work or school.

Methods: We developed an initial VRRS tool informed by previous studies, a scoping review to determine such a tool had not already been developed, and consultation with subject matter experts. We iteratively refined the tool on the basis of four rounds of interviews with young adults with cancer. The refined VRRS was then administered online to patients before they attended scheduled clinic …

Challenges And Opportunities In The Dissemination Of Scientific Content: Insights From Media Professionals In Jordan, Ruba Mustafa Mohammad, Mahmoud Ahmad Al-Rajabi, Hala Nabil Amr

Middle East Journal of Communication Studies

This study explored the challenges and opportunities in disseminating scientific content from the perspective of media experts in Jordan. Using a descriptive methodology and a questionnaire, the research aimed to understand the landscape better. The sample included 343 media experts holding master's degrees or higher in various media fields, along with several journalists actively working in the sector. The study revealed several key findings. Chief among them are the numerous obstacles hindering the dissemination of scientific content in Jordanian media. These include a lack of training for journalists on accessing new scientific information, a general absence of critical thinking skills …

The Degree Of Dependence Of The Jordanian Public On Social Media In Health Awareness Of Obesity Diseases (Survey Study), Jumana Irshiedat, Kamel Khurshid Murad

Middle East Journal of Communication Studies

The article aimed to reveal the dependency of social media platforms in educating the Jordanian public about obesity diseases and explore the cognitive, emotional, and behavioral effects on the Jordanian public. The survey method was adopted; an approach used in descriptive studies, and the questionnaire tool was used to a sample (357) of the Jordanian public in Amman. The study-produced results including the platform most used by the Jordanian public to receive knowledge about health awareness is WhatsApp in the first place, followed by Instagram and then Facebook. The results also showed that the health topics of most interest to …

The Energy Transition In The Jordanian Media, Hanan Al-Shiekh

Middle East Journal of Communication Studies

The study aimed to clarify the role of scientific journalism towards stimulating investment in environmentally friendly renewable energy sources, which is one of the necessary challenges facing the Kingdom of Jordan for economic growth and social development, due to its heavy dependence on energy imports, which reflects negatively on the Jordanian economy. This study is one of the descriptive studies that focuses on describing a specific topic, as it is in the current reality, in terms of its general and detailed characteristics, including variables, elements, relationships, and influences, which are among the most prominent features of scientific journalism in terms …

The Role Of Television In Increasing Knowledge About Health Issues Among Jordanian Citizens, Izzat Mohammad Hijab, Ahmad Oreqat, Hani Al-Badri

Middle East Journal of Communication Studies

This study explores the role of TV health programs in increasing the knowledge of Jordanian citizens about health issues. The study was based on the social survey methodology through a questionnaire distributed to a sample of 200 people from the city of Amman watching Jordan Television (JTV) health programs. The researcher concluded the following; a high watching for health programs, and that the Jordanians watch health programs with their family at home. The study also confirmed that the Jordanian benefits from health programs provided by JTV, and these programs are important and essential in educating citizens in health issues. Finally, …

Spatiotemporal Patterns Of Chlorophyll-A Concentration In A Hypersaline Lake Using High Temporal Resolution Remotely Sensed Imagery, R. Douglas Ramsey, Soren M. Brothers, Melissa Cobo, Wayne A. Wurtsbaugh

Wildland Resources Faculty Publications

The Great Salt Lake (GSL) is the largest saline lake in the Western Hemisphere. It supports billion-dollar industries and recreational activities, and is a vital stopping point for migratory birds. However, little is known about the spatiotemporal variation of phytoplankton biomass in the lake that supports these resources. Spectral reflectance provided by three remote sensing products was compared relative to their relationship with field measurements of chlorophyll a (Chl a). The MODIS product MCD43A4 with a 500 m spatial resolution provided the best overall ability to map the daily distribution of Chl a. The imagery indicated significant spatial …

Role Of An Smc-Like Protein, Recn, In Reca-Mediated Homology Search During Double-Strand Break Repair Via Homologous Recombination, Afroze Abdehaque Chimthanawala

Theses and Dissertations

The most deleterious form of DNA damage is the formation of a double-strand break (DSB). If left unrepaired or repaired incorrectly, DSBs can result in genome rearrangements, mutations, or even cell death. Hence, cells across domains of life have resorted to homologous recombination (HR) for the faithful repair of DSBs. A critical step in homologous recombination is the search for the intact homologous sequence by the break ends, termed ‘homology search’. This process is not well understood in vivo, especially when the break site and intact homologous template are not positioned adjacently.

Spatial reorganization of chromosomes is maintained by structural …

Registration Of Tx3499-Tx3503 Sorghum Germplasm Resistant To Acetyl-Coenzyme A Carboxylase-Inhibiting Herbicides, Daniel Crozier, Muthukumar Bagavathiannan, George L. Hodnett, Jason K. Norsworthy, Kayla A. Beechinor, William L. Rooney

Crop, Soil and Environmental Sciences Faculty Publications and Presentations

The sorghum [Sorghum bicolor (L.) Moench ssp. bicolor] germplasm lines Tx3499-Tx3503 (Reg. no. GP-948-Reg. no. GP-952, PI 706257-PI 706261) were developed by the Texas A&M AgriLife Research sorghum breeding program in 2024. These lines are resistant to acetyl-coenzyme A carboxylase (ACCase)-inhibiting herbicides used for post-emergence control of common grass weeds. Genetic resistance to aryloxyphenoxypropionate and phenylpyrazoline class herbicides was introgressed into Tx3499-Tx3503 from a donor line that contained a single partially dominant gene. These lines represent diverse pedigrees and an array of combinations of grain color, plant color, maintenance of sterility or restoration of fertility to A1 male-sterile cytoplasm, as …

01.27.2025 Ored Connect, Liz Williamson

ORED Newsletter

Data Science for Addiction Research

UM research and related activities as a new presidential administration transition occurs

Chemotherapy In Synergy With Innate Immune Agonists Enhances T Cell Priming For Checkpoint Inhibitor Treatment In Pancreatic Cancer, Nan Niu, Keyu Li, Junke Wang, Vanessa Funes, Birginia Espinoza, Pan Li, Jianxin Wang, Melissa Lyman, Mengni He, Brian Herbst, Michael Wichroski, Ruslan Novosiadly, Sami Shoucair, Yiping Mou, Lei Zheng

Faculty, Staff and Student Publications

Background: The combination of conventional chemotherapy and immune checkpoint inhibitors (ICIs) has been unsuccessful for pancreatic ductal adenocarcinoma (PDAC). Administration of maximum tolerated dose of chemotherapy drugs may have immunosuppressive effects.

Methods: We thus tested, by using the preclinical model of PDACs including the genetically engineered mouse KPC spontaneous pancreatic tumor model and the pancreatic KPC tumor orthotopic implant model, the combinations of synthetic innate immune agonists including STING and NLRP3 agonist, respectively, and ICIs with or without chemotherapy.

Results: We found that innate agonists potentiate the role of chemotherapy in inducing effector T cells and subsequently to prime the …

Development And Validation Of Natural Language Processing Algorithms In The Enact National Electronic Health Record Research Network, Yanshan Wang, Jordan Hilsman, Chenyu Li, Michele Morris, Paul M Heider, Sunyang Fu, Min Ji Kwak, Andrew Wen, Joseph R Applegate, Liwei Wang, Elmer Bernstam, Hongfang Liu, Jack Chang, Daniel R Harris, Alexandria Corbeau, Darren Henderson, John D Osborne, Richard E Kennedy, Nelly-Estefanie Garduno-Rapp, Justin F Rousseau, Chao Yan, You Chen, Mayur B Patel, Tyler J Murphy, Bradley A Malin, Chan Mi Park, Jungwei W Fan, Sunghwan Sohn, Sandeep Pagali, Yifan Peng, Aman Pathak, Yonghui Wu, Zongqi Xia, Salvatore Loguercio, Steven E Reis, Shyam Visweswaran

Faculty, Staff and Student Publications

Electronic health record (EHR) data are a rich and invaluable source of real-world clinical information, enabling detailed insights into patient populations, treatment outcomes, and healthcare practices. The availability of large volumes of EHR data are critical for advancing translational research and developing innovative technologies such as artificial intelligence. The Evolve to Next-Gen Accrual to Clinical Trials (ENACT) network, established in 2015 with funding from the National Center for Advancing Translational Sciences (NCATS), aims to accelerate translational research by democratizing access to EHR data for all Clinical and Translational Science Awards (CTSA) hub investigators. The present ENACT network provides access to …

Immuno-Bioinformatics Study Of Secondary Metabolites From Watercress Nasturtium Officinale As Immunostimulant In Whiteleg Shrimp Litopenaeus Vannamei By Targeting Peroxinectin And Scavenger Receptor Class B, Qurrota A’Yunin, Muhaimin Rifa’I, Maftuch Maftuch, Yoga Dwi Jatmiko, Muhammad Hermawan Widyananda, Triyanto Triyanto, Muhammad Tegar Handrianto

Karbala International Journal of Modern Science

The viral disease affects the whiteleg shrimp (Litopenaeus vannamei) and causes losses. Immunostimulants are recognized as a more environmentally friendly approach to disease management and antimicrobial properties of aquatic organisms. Watercress (Nasturtium officinale) is an aquatic plant species that contains antioxidant properties that can protect the organism from disease and boost the immune system. Shrimp rely heavily on innate immunity to combat infectious agents due to their absence of adaptive immunity. Peroxinectin and scavenger receptor class B (SRB) play essential roles in the shrimp defense system. This research employs a bioinformatic to assess the immunostimulatory potential …

Development And Characterization Of Cd44-Targeted X-Aptamers With Enhanced Binding Affinity For Cancer Therapeutics, Hongyu Wang, Weiguo He, Miguel-Angel Elizondo-Riojas, Xiaobo Zhou, Tae Jin Lee, David G Gorenstein

Faculty, Staff and Student Publications

CD44, a pivotal cell surface molecule, plays a crucial role in many cellular functions, including cell-cell interactions, adhesion, and migration. It serves as a receptor for hyaluronic acid and is involved in lymphocyte activation, recirculation, homing, and hematopoiesis. Moreover, CD44 is a commonly used cancer stem cell marker associated with tumor progression and metastasis. The development of CD44 aptamers that specifically target CD44 can be utilized to target CD44-positive cells, including cancer stem cells, and for drug delivery. Building on the primary sequences of our previously selected thioaptamers (TAs) and observed variations, we developed a bead-based X-aptamer (XA) library by …

Genome-Wide Profiling Of Trna Modifications By Induro-Trnaseq Reveals Coordinated Changes, Yuko Nakano, Howard Gamper, Henri Mcguigan, Sunita Maharjan, Jiatong Li, Zhiyi Sun, Erbay Yigit, Sebastian Grünberg, Keerthana Krishnan, Nan-Sheng Li, Joseph Piccirilli, Ralph Kleiner, Nicole Nichols, Brian Gregory, Ya-Ming Hou

Department of Biochemistry and Molecular Biology Faculty Papers

While all native tRNAs undergo extensive post-transcriptional modifications as a mechanism to regulate gene expression, mapping these modifications remains challenging. The critical barrier is the difficulty of readthrough of modifications by reverse transcriptases (RTs). Here we use Induro-a new group-II intron-encoded RT-to map and quantify genome-wide tRNA modifications in Induro-tRNAseq. We show that Induro progressively increases readthrough over time by selectively overcoming RT stops without altering the misincorporation frequency. In a parallel analysis of Induro vs. a related RT, we provide comparative datasets to facilitate the prediction of each modification. We assess tRNA modifications across five human cell lines and …

Influence Of High Eimeria Tenella Immunization Dosages On Total Oocyst Output And Specific Antibodies Recognition Response In Hybrid Pullets (Gallus Gallus)-A Pilot Study, Marco A. Juarez-Estrada, Guillermo Tellez-Isaias, Victor M. Petrone-Garcia, Amanda Gayosso-Vazquez, Xochitl Hernandez-Velasco, Rogelio A. Alonso-Morales

Political Science Faculty Publications and Presentations

Background: Two high primary-immunization doses of a wild-type E. tenella strain were assessed in healthy pullets (5K versus 10K sporulated oocysts/bird) to understand the effects of coccidia infection. Methods: Acquired immunity was evaluated following primary immunization and two booster doses with the homologous strain. Total oocyst shedding, clinical signs, and viability of every bird/group after each immunization/booster were recorded. Indirect ELISA measured the time course of humoral responses from each immunization group against sporozoite and second-generation merozoite of E. tenella. Antigen pattern recognition on these two asexual zoite stages of E. tenella was analyzed using Western blotting with antibodies from …