Life Sciences Commons^™

Bayesian Population Pharmacokinetic Modeling Of Ondansetron For Neonatal Opioid Withdrawal Syndrome, Kevin Lam, John Mondick, Gary Peltz, Manhong Wu, Walter Kraft

Department of Pharmacology, Physiology, and Cancer Biology Faculty Papers

Ondansetron is an anti-emetic 5-HT3 receptor antagonist being investigated for treating neonatal opioid withdrawal syndrome (NOWS). Sparse PK data were analyzed from a multicenter, double-blind clinical trial with 98 mother/neonate dyads. Pregnant women with opioid use disorder were randomized to receive either placebo or ondansetron 8 mg intravenously within 4 h of delivery. Neonates born to mothers who were randomized to ondansetron received 0.07 mg/kg orally once every 24 h for up to five doses. Using current PK data, model parameters from a two-compartmental structural model from the literature (i.e., a priori model) were updated with the Metropolis-Hastings Markov-chain Monte …

Lumpy Heterogeneity In Groundwater Service Values And Time Preferences, Kent F. Kovacs, Heather Snell, Brandon R. Mcfadden, Rodolfo M. Nayga Jr.

Agricultural Economics and Agribusiness Faculty Publications and Presentations

From a choice experiment about groundwater management, we consider time preferences and groundwater service values through sociodemographic characteristics using a model called the discounted latent class model. A policy-motivated latent class has a higher income, greater familiarity with an aquifer, and more concern for the risks that groundwater decline poses to people and the environment. A business-as-usual latent class has a conservative ideology. Exponential discounting is preferred, and the discount rate is larger for the policy group than the business-as-usual group. Other models we use include the discounted mixed logit model and the discounted logit-mixed logit model.

Attenuation Of Vitamin E On Haloxyfop-R-Methyl Ester-Induced Hepatic Oxidative Stress In Orally Exposed Rats., Omolara D. Falodun

STEM for Success Resources

No abstract provided.

Determinants Of Market Participation Among Milk Producers In Kyrgyzstan, Akhmadjon Nurullaev, Eunchun Park, Bruce L. Ahrendsen, Stijn Speelman, Lawson Connor

Agricultural Economics and Agribusiness Faculty Publications and Presentations

Numerous studies have been conducted to comprehend the economic importance of market participation for producers. However, a significant gap remains, particularly in Central Asia. This study assesses market participation drivers for Kyrgyzstan's milk producers using the Life in Kyrgyzstan dataset, encompassing a comprehensive nationwide farm-level survey. Findings reveal that household assets boost market entry and sales volume, whereas distance from markets discourages participation but can increase sales for active sellers, reflecting transportation cost strategies. The insights inform policy interventions benefiting rural small-scale milk producers in Central Asian countries that share similar cultural backgrounds.

Marine Protected Species Identification Guide 2025, Department Of Primary Industries And Regional Development, Western Australia

Fisheries Occasional Publications

The Western Australian aquatic environment is home to a number of species that are protected under international agreements and national or state legislation. Many of these species occur in areas where fishing takes place, sometimes resulting in interactions between fishers and protected species. An interaction is considered to be any physical contact between a person, fishing vessel or fishing gear and a protected species, including captures, entanglements and collisions.

This guide has been developed to help you identify some common marine protected species in Western Australia that you may encounter while fishing.

It has been divided into 5 sections:

• …

Coverage Matters: Identifying And Mitigating Sampling Frame Issues In Recreational Fishing Surveys, Steve Taylor, Annica De Groote, Kieran Hyder, Jon Volstad, B W. Hartill, J Foster, R Andrews, Karina L. Ryan

Fisheries Research Articles

Surveys play an integral part in monitoring and maintaining sustainable recreational fisheries. For any probabilistic survey, the selection of a sampling frame (e.g., list of individuals or fishers) is an important decision because it influences the ability to provide unbiased estimates of recreational catch and effort. Undercoverage occurs when units of the target population (i.e., the population of interest) are missing from the frame population. This error can undermine the reliability of research advice generated from survey estimates. In this review, we: (i) define six sampling frame configurations that are commonly applied in probabilistic recreational fishing surveys; (ii) synthesise how …

Gdp-Mannose 4,6-Dehydratase Is A Key Driver Of Mycn-Amplified Neuroblastoma Core Fucosylation And Tumorigenesis, Beibei Zhu, Michelle G. Pitts, Michael D. Buoncristiani, Lindsay T. Bryant, Oscar Lopez-Nunez, Juan P. Gurria, Cameron Shedlock, Roberto Ribas, Shannon Keohane, Jinpeng Liu, Chi Wang, Matthew S. Gentry, Nathan R. Shelman, Derek B. Allison, B. Mark Evers, Ramon C. Sun, Eric J. Rellinger

Markey Cancer Center Faculty Publications

MYCN-amplification is a genetic hallmark of ~40% of high-risk neuroblastomas (NBs). Altered glycosylation is a common feature of adult cancer progression, but little is known about how genetic signatures such as MYCN-amplification alter glycosylation profiles. Herein, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) revealed increased core fucosylated glycan abundance within neuroblast-rich regions of human MYCN-amplified NB tumors. GDP-mannose 4,6-dehydratase (GMDS) is responsible for the first-committed and rate-limiting step of de novo GDP-fucose synthesis. High GMDS expression was found to be associated with poor patient survival, advanced stage disease, and MYCN-amplification in human NB tumors. Chromatin immunoprecipitation and promoter reporter assays …

A Microrna-Regulated Transcriptional State Defines Intratumoral Cd8+ T Cells That Respond To Immunotherapy, William W. Tang, Ben Battistone, Kaylyn M. Bauer, Allison M. Weis, Cindy Barba, Muhammad Zaki Hidayatullah Fadlullah, Arevik Ghazaryan, Van B. Tran, Soh-Hyun Lee, Z. Busra Agir, Morgan C. Nelson, Emmanuel Stephen Victor, Amber Thibeaux, Colton Hernandez, Jacob Tantalla, Aik C. Tan, Dinesh Rao, Matthew Williams, Micah J. Drummond, Ellen J. Beswick, June L. Round, H. Atakan Ekiz, Warren P/ Voth, Ryan M. O’Connell

Markey Cancer Center Faculty Publications

The rising incidence of advanced-stage colorectal cancer (CRC) and poor survival outcomes necessitate new and effective therapies. Immune checkpoint inhibitors (ICIs), specifically anti-PD-1 therapy, show promise, yet clinical determinants of a positive response are suboptimal. Here, we identify microRNA-155 (miR-155) as necessary for CD8 + T cell-infiltrated tumors through an unbiased in vivo CRISPR-Cas9 screen identifying functional tumor antigen-specific CD8+ T cell-expressed microRNAs. T cell miR-155 is required for anti-PD-1 responses and for a vital intratumor CD8 + T cell differentiation cascade by repressing Ship-1, inhibiting Tcf-1 and stemness, and subsequently enhancing Cxcr6 expression, anti-tumor immunity, and effector functions. Based …

Staying Sane In The Membrane: Neutral Sphingomyelinase 2 As A Master Regulator Of Plasma Membrane Ceramide, Zainuddin Quadri, Erhard Bieberich

Markey Cancer Center Faculty Publications

Ceramide, a key signaling sphingolipid in the plasma membrane, plays a pivotal role in fundamental cellular processes such as adhesion, polarity, and programmed cell death. The generation of plasma membrane ceramide is largely attributed to the activity of two types of sphingomyelinases: neutral sphingomyelinase 2 (nSMase2, Smpd3) and acid sphingomyelinase (aSMase, Smpd1). While many studies have explored ceramide generation following experimental activation of these enzymes, the mechanisms governing basal or steady- state ceramide levels have remained poorly understood. Using an innovative mass spectrometry approach developed in the Canals’ lab, the team has quantified the distinct contributions of nSMase2 and aSMase …

Bilirubin Bioconversion To Urobilin In The Gut-Liver-Kidney Axis: A Biomarker For Insulin Resistance In The Cardiovascular-Kidney-Metabolic (Ckm) Syndrome, Zachary A. Kipp, Olufunto O. Badmus, David E. Stec, Brantley Hall, Terry D. Hinds Jr.

Markey Cancer Center Faculty Publications

The rising rates of obesity worldwide have increased the incidence of cardiovascular disease (CVD), making it the number one cause of death. Higher plasma bilirubin levels have been shown to prevent metabolic dysfunction and CVD. However, reducing levels leads to deleterious outcomes, possibly due to reduced bilirubin half-life that escalates the production of its catabolized product, urobilinogen, produced by gut bacteria and naturally oxidized to urobilin. Recent findings suggest that the involvement of the microbiome catabolism of bilirubin to urobilin and its absorption via the hepatic portal vein contributes to CVD, suggesting a liver-gut axis involvement. We discuss the studies …

Ddo-Adjuvanted Influenza A Virus Nucleoprotein Mrna Vaccine Induces Robust Humoral And Cellular Type 1 Immune Responses And Protects Mice From Challenge, Victoria Gnazzo, Hanaa Saleh, Ítalo A. Castro, Adrianus C. M. Boon, Amelia K. Pinto, James D. Brien, Carolina B. López

Markey Cancer Center Faculty Publications

A challenge in viral vaccine development is to produce vaccines that generate both neutralizing antibodies to prevent infection and cytotoxic CD8+ T-cells that target conserved viral proteins and can eliminate infected cells to control virus spread. mRNA technology offers an opportunity to design vaccines based on conserved CD8-targeting epitopes, but achieving robust antigen-specific CD8+ T-cells remains a challenge. Here, we tested the viral-derived oligonucleotide DDO268 as an adjuvant in the context of a model influenza A virus (IAV) nucleoprotein (NP) mRNA vaccine in C57BL/6 mice. DDO268 when co-packaged with mRNA in lipid nanoparticles is sensed by RIG I-like receptors and …

Gdp-Mannose 4,6-Dehydratase Is A Key Driver Of Mycn- Amplified Neuroblastoma Core Fucosylation And Tumorigenesis, Beibei Zhu, Michelle G. Pitts, Michael D. Buoncristiani, Lindsay T. Bryant, Oscar Lopez-Nunez, Juan P. Gurria, Cameron Shedlock, Roberto Ribas, Shannon Keohane, Jinpeng Liu, Chi Wang, Matthew S. Gentry, Nathan R. Shelman, Derek B. Allison, B. Mark Evers, Ramon C. Sun, Eric J. Rellinger

Markey Cancer Center Faculty Publications

MYCN-amplification is a genetic hallmark of ~40% of high-risk neuroblastomas (NBs). Altered glycosylation is a common feature of adult cancer progression, but little is known about how genetic signatures such as MYCN-amplification alter glycosylation profiles. Herein, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) revealed increased core fucosylated glycan abundance within neuroblast-rich regions of human MYCN-amplified NB tumors. GDP-mannose 4,6-dehydratase (GMDS) is responsible for the first-committed and rate-limiting step of de novo GDP-fucose synthesis. High GMDS expression was found to be associated with poor patient survival, advanced stage disease, and MYCN-amplification in human NB tumors. Chromatin immunoprecipitation and promoter reporter assays …

Evaluating The Generalisability Of Region-Naïve Machine Learning Algorithms For The Identification Of Epilepsy In Low-Resource Settings, Ioana Duta, Symon M. Kariuki, Anthony Ngugi, Angelina Kakooza Mwesige, Honorati Masanja, Daniel M. Mwanga, Seth Owusu-Agyei, Ryan Wagner, J Helen Cross, Josemir W. Sander W. Sander

Population Health, East Africa

Objectives: Approximately 80% of people with epilepsy live in low- and middle-income countries (LMICs), where limited resources and stigma hinder accurate diagnosis and treatment. Clinical machine learning models have demonstrated substantial promise in supporting the diagnostic process in LMICs by aiding in preliminary screening and detection of possible epilepsy cases without relying on specialised or trained personnel. How well these models generalise to naïve regions is, however, underexplored. Here, we use a novel approach to assess the suitability and applicability of such clinical tools to aid screening and diagnosis of active convulsive epilepsy in settings beyond their original training contexts. …

National Ffa Parliamentary Procedure And Conduct Of Chapter Meetings Leadership Development Events Presentation Generator, P. Troy White

Department of Agricultural Leadership, Education, and Communication: Faculty Publications

A Microsoft Excel macro-enabled workbook that produces presentation cards for the National FFA Organization's Parliamentary Procedure and Conduct of Chapter Meetings Leadership Development Events.

Each set of cards also creates a judges help sheet for the motions with proper verbiage and the Standard Descriptive Characteristics as listed in Robert's Rules of Order Newly Revised (12th edition).

The workbook uses randomization to create set cards from a list of over 300 possible main motions from a variety of internet sources. The 2024 list of eligible motions for both events was used for the permissible motions.

Brain Transcriptomics Of A Social Challenge And Maternal Aggression In Incubating Female Tree Swallows, Emily J. Levy, Elizabeth M. George, Douglas B. Rusch, Aaron Buechlein, Kimberly A. Rosvall

Biology Faculty Publications

Aggressive behavior is ubiquitous across many contexts, including defense of territories, mates, and offspring. For decades, researchers have detailed the effect of aggressive behavior on physiology, but our understanding of these mechanisms in females lags behind that of males, despite the fact that female aggression is widespread, particularly in the context of maternal defense of eggs or offspring (i.e., maternal aggression). Here, we measured effects of a social challenge on brain gene expression in free-living incubating females. We hypothesized that the social challenge would generate at least one of three transcriptomic effects: (1) sensitizing the brain to otherwise low levels …

Host Traits And Temperature Predict Biogeographical Variation In Seagrass Disease Prevalence, F.R. Schenck, J.K. Baum, K.E. Boyer, J.E. Duffy, F.J. Fodrie, J. Gaeckle, Torrie Hanley, C.M. Hereu, K.A. Hovel, P. Jorgensen, D.L. Martin, N.E. O’Connor, B.J. Peterson, J.J. Stachowicz, A.R. Hughes

Biology Faculty Publications

Diseases are ubiquitous in natural systems, with broad effects across populations, communities and ecosystems. However, the drivers of many diseases remain poorly understood, particularly in marine environments, inhibiting effective conservation and management measures. We examined biogeographical patterns of infection in the foundational seagrass Zostera marina by the parasitic protist Labyrinthula zosterae, the causative agent of seagrass wasting disease, across >20° of latitude in two ocean basins. We then identified and characterized relationships among wasting disease prevalence and a suite of host traits and environmental variables. Host characteristics and transmission dynamics explained most of the variance in prevalence across our survey, …

Factors Influencing The Adoption Of Agroecological Vegetable Cropping Systems By Smallholder Farmers In Tanzania, Essy C. Kirui, Michael M. Kidoido, Komivi S. Akutse, Rosina Wanyama, Simon B. Boni, Thomas Dubois, Fekadu F. Dinssa, Daniel M. Mutyambai

All Peer-Reviewed Publications

Vegetable production is vital to smallholder farmers, who often struggle to overcome pests, diseases, and extreme weather. Agroecological cropping systems offer sustainable solutions to these issues but their adoption rates in Tanzania remain low. This study examines the factors influencing smallholder farmers’ adoption of selected agroecological cropping systems for vegetable production in Tanzania, which remains underexplored. Using a multistage sampling technique, cross-sectional data were gathered from 525 crucifer and traditional African vegetable farming households within the Arusha and Kilimanjaro regions. Multivariate probit regression analysis, which accounts for the simultaneous adoption of multiple systems, revealed several significant variables influencing adoption. The …

Climate Effects On Honey Bees Can Be Mitigated By Beekeeping Management In Kenya, Malena Sibaja Leyton, H. Michael G. Lattorff, Nkoba Kiatoko, Fabrice Requier

All Peer-Reviewed Publications

In recent decades, worldwide concerns about the health of honey bees motivated the development of surveys to monitor the colony losses, of which Sub-Saharan Africa has had limited representation. In the context of climate change, understanding how climate affects colony losses has become fundamental, yet literature on this subject is scarce. For the first time, we conducted a survey to estimate the livestock decrease of honey bee colonies in Kenya for the year 2021–2022 to explore the effects of environmental conditions, such as temperature and precipitation, on livestock decrease. We define “livestock decrease” from the beekeeper's perspective, including dead colonies …

The Mc4r Agonist, Setmelanotide, Is Associated With An Improvement In Hypercapnic Chemosensitivity And Weight Loss In Male Mice, Sarah N. Framnes-Deboer, Deanna M. Arble, Athena Rivera

Biological Sciences Faculty Research and Publications

Obesity increases the risk of respiratory diseases that reduce respiratory chemosensitivity, such as Obesity Hypoventilation Syndrome and sleep apnea. Recent evidence suggests that obesity-related changes in the brain, including alterations in melanocortin signaling via the melanocortin-4 receptor (MC4R), may underly altered chemosensitivity. Setmelanotide, an MC4R agonist, causes weight loss in both humans and animal models. However, it is unknown the extent to which setmelanotide affects respiratory chemosensitivity independent of body weight loss. The present study uses diet-induced obese, male C57bl/6 J mice to determine the extent to which acute setmelanotide treatment affects the hypercapnic ventilatory response (HCVR). We find that …

Hydrolysis Of The Acetyl-Coa Allosteric Activator By Staphylococcus Aureus Pyruvate Carboxylase, Amanda J. Laseke, Jeremy R. Lohman, Martin St. Maurice

Biological Sciences Faculty Research and Publications

Pyruvate carboxylase (PC) catalyzes the carboxylation of pyruvate to oxaloacetate which serves as an important anaplerotic reaction to replenish citric acid cycle intermediates. In most organisms, the PC-catalyzed reaction is allosterically activated by acetyl-coenzyme A. It has previously been reported that vertebrate PC can catalyze the hydrolysis of acetyl-CoA, offering a potential means for the enzyme to attenuate its allosteric activation. However, in the years since this initial report, there has been no further investigation of this phenomenon. The allosteric binding site for acetyl-CoA is now well characterized, enabling more detailed studies on acetyl-CoA hydrolysis at the allosteric site. Here, …

An Erp Study Of Attentional Blink And Inhibition Of Return, Caesar Ekya

Dissertations, Theses, and Capstone Projects

Attention can be allocated over time and space and it has long been speculated whether these are a part of the same or different attentional mechanisms. Attentional Blink (AB) and Inhibition of Return (IOR) are cognitive phenomena that are used in experimental settings to study temporal attention (AB) and spatial attention (IOR) respectively. Though known for being representative of different types of attention, they nevertheless display similar characteristics, making them intriguing choices to study the interplay between temporal and spatial attention. For this study we chose a dual-stream rsvp paradigm that would elicit both AB and IOR within the same …

Solvation Thermodynamic Mapping Approaches For Computer-Aided Drug Discovery, Vjay Molino

Dissertations, Theses, and Capstone Projects

The drug discovery process is inherently long and costly, prompting the development and integration of computational tools to mitigate these challenges. One of area explored in computer-aided drug discovery is to understand the role of solvation in protein-drug binding and to develop computational tools and methods that can be integrated in the drug discovery process. Grid Inhomogeneous Solvation Theory (GIST) is a tool that provides a framework for mapping solvation thermodynamiproperties of water molecules on the protein surface.This thesis explores the use of GIST in generating pharmacophore models and elucidates the role of solvation in protein structural fluctuations.

One of …

Evidence For Interactive Effects Of Inhibitory And Prospective Intolerance Of Uncertainty And Stimulus-Level Uncertainty On Threat Safety Discrimination Learning, Kyoko Yazaki

Dissertations, Theses, and Capstone Projects

Fear is a fundamental component of human learning, critical for survival, and extensively studied through Pavlovian conditioning paradigms (LeDoux & Pine, 2016). This thesis examines the interplay between stimulus-level uncertainty and individual differences in intolerance of uncertainty (IU) on threat-safety discrimination learning. Using a within-subject design, participants were conditioned to certain (100% reinforcement), uncertain (50% reinforcement), and safety (0% reinforcement) cues through the pairing of colored visual stimuli with aversive electrical shocks. Discrimination learning was assessed using both implicit (SCR) and explicit (self-reported US expectancy estimates and unpleasantness ratings) measures.

The present study (n = 102) replicated previous findings showing …

Characterizing Malaria Infections In Caribbean Lizards: Parasite Niche Expansion & Host Response, Sarah J. Pangburn

Dissertations, Theses, and Capstone Projects

Malaria is historically one of the most severe parasitic human diseases and is still responsible for over half a million deaths annually. Plasmodium species, the causative agents of malaria, are often characterized by two hallmark features of their asexual stages: the invasion of vertebrate red blood cells and the presence of hemozoin pigment as a byproduct of host hemoglobin digestion. Despite their evolutionary relatedness to mammalian Plasmodium, wildlife malaria parasites are understudied, leaving their vast genetic diversity to be explored. Lizard-infective malaria parasites, for example, include over 100 species of Plasmodium and present a diverse array of phenotypes, some …

Quantified Lives: Data, Bias, And The Cost Of Categorization, Colin F. Geraghty

Dissertations, Theses, and Capstone Projects

This project critically examines how statistical methods and data visualization have historically been used to categorize, rank, and control human populations. By exploring the works of key figures like Francis Galton, Karl Pearson, and R.A. Fisher, it traces the evolution of categorization frameworks from their eugenic roots to modern applications in artificial intelligence and global metrics like the World Happiness Report. Through interactive visualizations and historical analysis, the project invites users to question the legitimacy of these frameworks and reflect on their impact on society. At its core, this work emphasizes the need to move beyond rigid classification systems, challenging …

The Alphaproteobacterial General Stress Response: Investigating Protein Structure, Dynamics, And Regulatory Mechanisms, Danielle Swingle

Dissertations, Theses, and Capstone Projects

Bacteria are relatively simple organisms that face a wide variety of environmental challenges. Their survival is determined by their ability to sense and respond to changes in their surroundings. Alphaproteobacteria commonly accomplish this using the general stress response (GSR). During the first step in this system, a signal is detected by a sensor histidine kinase (HK), modulating autophosphorylation within its kinase domain. HKs involved in the GSR generally belong to the HWE/HisKA2 family, a lesser studied group of HKs with noncanonical features. The signal is propagated when the HK phosphorylates the next protein in the pathway, PhyR. Once activated, PhyR …

Category Learning Reorganizes Internal Representations Of Visual Artworks, Aishwarya Gurung

Dissertations, Theses, and Capstone Projects

How are categories of art internally represented, and how can we capture those representations systematically? Investigating category learning in the context of complex, multidimensional stimuli like visual artworks presents unique challenges due to their inherent variability and perceptual complexity. Artworks engage multiple cognitive processes and are shaped by both sensory-driven mechanisms and top-down influences like prior knowledge, making them uniquely suited for studying how structured categorical knowledge interacts with perceptual and aesthetic judgments. The present study addresses these questions by utilizing machine-learning tools to construct and implement a two-dimensional similarity space of 400 existing artworks painted by two artists from …

The Role Of Secondary And Tertiary Structure In The Cap-Independent Translation Of Fgf-9 And Hif-1-Alpha, Amanda Michelle Whittaker

Dissertations, Theses, and Capstone Projects

Under normoxic conditions, eukaryotes initiate translation of RNA through eIF4E recognition of the 5’ cap. However, under cellular stress, eukaryotic translation must be initiated through a 4E-independent, or “cap-independent” mechanism, involving eukaryotic initiation factor 4G (eIF4G) binding directly to the 5’ untranslated regions (5’ UTR) of the RNA. eIF4G binding then recruits the ribosome to the transcript. While this mechanism is useful for translation of apoptotic transcripts and transcripts involved in cell survival, cap-independent translation is also utilized by oncogenic RNA for tumorigenesis. Previous work by our lab and others has categorized this recruitment and initiation mechanism as either internal-ribosome-entry-site …

Constructing A Parameterized Stimuli Space Suitable To Induce Controlled Changes In Human And Machine Perceptual Systems, Andrew Frankel

Dissertations, Theses, and Capstone Projects

Traditional computer vision applications focus on objective tasks, such as object detection, classification, or segmentation. Much of human experience is inherently subjective, such as our personal response to artwork. Individualized experiences are challenging to replicate in a controlled laboratory environment, as those experiences depend on the unique history and internal models of the observer. This work presents a technique to construct a 2-dimensional parameterized stimuli space suitable to induce controlled changes in the perceptual systems of both human and machine observers. Our contributions are threefold:

1. Using a balanced dataset of 8,800 fine art images spanning 11 art movements and 88 …

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Tracing The Sources Of Nitrogen In Submarine Groundwater Discharge And Primary Producers In The Great South Bay Estuary, Nia H. Rene

Dissertations, Theses, and Capstone Projects

The primary aim of this research was to elucidate the impact of submarine groundwater discharge (SGD) and septic effluent on coastal primary producers in the Great South Bay (GSB). The study focused on three main objectives: assessing the impact of precipitation patterns on SGD, tracing nitrogen sources into the GSB, and determining the impact of these nitrogen sources on coastal primary producers. The research revealed that current precipitation patterns significantly influence SGD across different seasons, highlighting the seasonal variability in groundwater discharge and its implications for nutrient loading in coastal waters. A suite of tracers was employed to identify and …