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Full-Text Articles in Life Sciences

Inhibition Of Lysine Deacetylase Activity Impacts Formation Of The Vitamin D Receptor Activation Complex, Shannon Dwyer Feb 2025

Inhibition Of Lysine Deacetylase Activity Impacts Formation Of The Vitamin D Receptor Activation Complex, Shannon Dwyer

Theses and Dissertations

The vitamin D endocrine system is responsible for the regulation of many biological processes including bone metabolism, calcium homeostasis, cell proliferation and cell differentiation. Alterations to the vitamin D signaling pathway are associated with several diseases including bone diseases, diabetes, cardiovascular diseases, autoimmune diseases, and cancer. Vitamin D precursors are chemically modified to become the biologically active hormone, calcitriol. Calcitriol binds to the vitamin D receptor (VDR), a member of the nuclear hormone receptor (NHR) superfamily. VDR forms a heterodimer with retinoid X receptor (RXR) and together they bind to promoters containing vitamin D response elements (VDREs) to activate transcription …


An Intensified Cereal Push-Pull System Reduces Pest Infestation And Confers Yield Advantages In High-Value Vegetables, Frank Chidawanyika, Evanson R. Omuse, Lavender O. Agutu, Jimmy O. Pittchar, Dickens Nyagol, Zeyaur R. Khan Feb 2025

An Intensified Cereal Push-Pull System Reduces Pest Infestation And Confers Yield Advantages In High-Value Vegetables, Frank Chidawanyika, Evanson R. Omuse, Lavender O. Agutu, Jimmy O. Pittchar, Dickens Nyagol, Zeyaur R. Khan

All Peer-Reviewed Publications

Crop diversification is associated with ecosystem services that can improve yield. We integrated tomatoes and kales within the cereal push-pull technology (PPT), to form the vegetable integrated push-pull (VIPP), and explored the influence of these cropping systems on pest and disease management, and subsequent yield of the vegetables. Aphids and diamondback moths (DBM), the major pests in kale production, together with grasshoppers were consistently lower in the VIPP plots. Low incidences and damage by leafminers, whiteflies and fruitflies on tomatoes were observed in VIPP plots compared to plots of tomato intercropped with maize (control). The severity of black rot and …


Evaluation Of High Tunnel Tomato Cultivars For Yield And Quality, Wenjing Guan Feb 2025

Evaluation Of High Tunnel Tomato Cultivars For Yield And Quality, Wenjing Guan

Midwest Vegetable Trial Reports

Tomatoes are a highly popular crop in Indiana's local food markets. They are most commonly grown in high tunnels to extend the harvest season and enhance yields. Beyond yield, fruit quality is a key factor for market success. This study evaluated the performance of eight determinant tomato cultivars grown in a high tunnel at the Southwest Purdue Agricultural Center in 2023.


Watershed Land Use Decreases The Nitrogen Limitation Of Benthic Algal Growth In Coastal Wetlands Of A Large Oligotrophic Lake, Matthew J. Cooper, Michele C. Wheeler Feb 2025

Watershed Land Use Decreases The Nitrogen Limitation Of Benthic Algal Growth In Coastal Wetlands Of A Large Oligotrophic Lake, Matthew J. Cooper, Michele C. Wheeler

Open Access Publishing Support Funded Articles

Introduction:

Identifying patterns in the primary limiting nutrients of basal trophic levels such as benthic algae can inform the prediction of potential ecological responses to anthropogenic nutrient loading. In coastal wetlands of the Laurentian Great Lakes, reduced concentrations of reactive nitrogen species such as ammonium and nitrate may limit algal growth, especially when nutrient loading is minimal. However, the response of benthic algae to macronutrient inputs remains understudied, especially in Lake Superior coastal wetlands.

Methods:

We conducted nutrient amendment assays using nutrient diffusing substrate devices in 25 coastal wetlands along the southwestern shore of Lake Superior in the spring, summer, …


S-Methyl-L-Cysteine Sulfoxide And Glucosinolate Levels In Australian-Sourced Brassica Vegetables Before And After Domestic Cooking, Caroline R. Hill, Armaghan Shafaei, Mary C. Boyce, Liezhou Zhong, Lois Balmer, Joshua R. Lewis, Jonathan M. Hodgson, Lauren C. Blekkenhorst Feb 2025

S-Methyl-L-Cysteine Sulfoxide And Glucosinolate Levels In Australian-Sourced Brassica Vegetables Before And After Domestic Cooking, Caroline R. Hill, Armaghan Shafaei, Mary C. Boyce, Liezhou Zhong, Lois Balmer, Joshua R. Lewis, Jonathan M. Hodgson, Lauren C. Blekkenhorst

Research outputs 2022 to 2026

Currently no data exists on S-methyl-L-cysteine sulfoxide (SMCSO) levels in Australian-sourced vegetables and limited data exists for glucosinolates. The effect of various cooking methods on the retention of SMCSO and glucosinolates in Australian-sourced vegetables is also limited. This study measured SMCSO and ten glucosinolates in a selection of Australian-sourced Brassica vegetables, both raw and after steaming. We additionally measured SMCSO and glucosinolate levels in broccoli after microwaving, stir-frying and boiling. SMCSO contributed greater dry weight (0.6–1.9 %) than total glucosinolates combined (0.3–1.2 %) in these raw cruciferous vegetables. SMCSO levels from highest to lowest were: Brussels sprouts > broccoli > red cabbage …


Product Testing: Research Shows Benefits Of Biostimulants May Vary By Variety, Prakriti Nepal, Youping Sun, Milena Oliveira Feb 2025

Product Testing: Research Shows Benefits Of Biostimulants May Vary By Variety, Prakriti Nepal, Youping Sun, Milena Oliveira

Plants, Soils and Climate Student Research

The use of plant biostimulants is showing some promise as a potential means of helping onion crops thrive despite certain environmental challenges, according to new research at Utah State University.


Arkansas Corn And Grain Sorghum Performance Tests 2024, J. F. Carlin, R. D. Bond, J. C. Mccoy, T. C. Swanson Feb 2025

Arkansas Corn And Grain Sorghum Performance Tests 2024, J. F. Carlin, R. D. Bond, J. C. Mccoy, T. C. Swanson

Arkansas Agricultural Experiment Station Research Series

Corn and grain sorghum performance tests are conducted each year in Arkansas by the University of Arkansas System Division of Agriculture. The tests provide information to companies marketing seed within the state and aid the Arkansas Cooperative Extension Service in formulating recommendations for producers.

The 2024 corn performance tests contained 44 hybrids and were conducted at the Northeast Rice Research and Extension Center (NERREC) at Harrisburg, the Lon Mann Cotton Research Station (LMCRS) near Marianna, the Rohwer Research Station (RRS) near Rohwer, and the Rice Research and Extension Center (RREC) near Stuttgart. The 2024 grain sorghum performance tests contained 16 …


Extracellular Vesicles Released By All Patients Contain Hne-Adducted Proteins: Implications Of Collateral Damage., Jenni Ho, Suriyan Sukati, Tamara Taylor, Sherry Carter, Brittany Fuller, Amy Marmo, Caryn Sorge, John D'Orazio, D Allan Butterfield, Subbarao Bondada, Heidi Weiss, Daret K St Clair, Luksana Chaiswing Feb 2025

Extracellular Vesicles Released By All Patients Contain Hne-Adducted Proteins: Implications Of Collateral Damage., Jenni Ho, Suriyan Sukati, Tamara Taylor, Sherry Carter, Brittany Fuller, Amy Marmo, Caryn Sorge, John D'Orazio, D Allan Butterfield, Subbarao Bondada, Heidi Weiss, Daret K St Clair, Luksana Chaiswing

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Off-target neuronal injury is a serious side-effect observed in cancer survivors. It has previously been shown that pediatric acute lymphoblastic leukemia (ALL) survivors have a decline in neurocognition compared to healthy age-matched counterparts. Elevated oxidative stress has been documented to be a mediator in off-target tissue damage in cancer survivors. Early detection of oxidative stress markers may provide an opportunity to prevent off-target tissue damage. Extracellular vesicles (EVs) have surfaced as a potential diagnostic tool due to molecular cargo they contain. We investigated the potential for EVs to be a sensitive indicator of oxidative stress and off-target tissue damage by …


Analysis Of 26 Studies Of The Impact Of Coconut Oil On Lipid Parameters: Beyond Total And Ldl Cholesterol, Mary T. Newport, Fabian M. Dayrit Feb 2025

Analysis Of 26 Studies Of The Impact Of Coconut Oil On Lipid Parameters: Beyond Total And Ldl Cholesterol, Mary T. Newport, Fabian M. Dayrit

Chemistry Faculty Publications

Coconut oil (CNO) is often characterized as an “artery-clogging fat” because it is a predominantly saturated fat that ostensibly raises total cholesterol (TChol) and LDL cholesterol (LDL-C). Whereas previous analyses assessed CNO based on the relative effects on lipid parameters against other fats and oils, this analysis focuses on the effects of CNO itself. Here, we review the literature on CNO and analyze 984 lipid profile data sets from 26 CNO studies conducted over the past 40 years. This analysis shows considerable heterogeneity among CNO studies regarding participant selection, the amount consumed, and the study duration. The analysis reveals that, …


Frequency-Dependent Assembly Processes Determine The Coexistence And Relative Abundance Of Tropical Plant Species, David M. Defilippis, Joseph A. Lamanna, Stefan A. Schnitzer Feb 2025

Frequency-Dependent Assembly Processes Determine The Coexistence And Relative Abundance Of Tropical Plant Species, David M. Defilippis, Joseph A. Lamanna, Stefan A. Schnitzer

Biological Sciences Faculty Research and Publications

Testing the extent to which ecological communities are structured by deterministic (niche-based) assembly processes, resulting in predictable species abundance and composition, is a fundamental goal of ecology. Here we use a 10-year dataset of 55,156 lianas comprising 86 species in an old-growth tropical forest in Panama to test whether community assembly is consistent with niche-based assembly processes. We find that species diversity and community composition was maintained because species conformed to four general requirements of coexistence theory: (1) species have negative conspecific frequency-dependent feedback that control their local population size; (2) species have a stronger negative effect on their own …


Long-Term Agricultural Management Reduces Abundance And Alters Community Structure Of Ground Beetles (Coleoptera: Carabidae), Cynthia M. Fiser, Nathan L. Haan, Douglas A. Landis Feb 2025

Long-Term Agricultural Management Reduces Abundance And Alters Community Structure Of Ground Beetles (Coleoptera: Carabidae), Cynthia M. Fiser, Nathan L. Haan, Douglas A. Landis

Entomology Faculty Publications

Carabid beetles (Coleoptera: Carabidae) are beneficial predators and bioindicators of ground-dwelling arthropod diversity in agricultural landscapes. We studied changes in community composition, activity density, and diversity of carabids in a long-term agroecosystem study spanning 30 years in the US Midwest. We contrasted carabid community metrics measured by pitfall trap sampling in two conservation-oriented treatments in a corn-soybean-wheat rotation. One treatment uses conventional practices but with reduced agrochemical inputs (Reduced Input), while the other has no synthetic inputs (Biologically Based). Since a 1994–95 study on the same site, in 2019 overall carabid activity density had declined a minimum of 58–76 % …


Life Span Policies And Macroeconomic Transition Will Help The 21st-Century Brain Health Revolution In Developing Countries, Cyprian Mostert, Chinedu Momoh, Manasi Kumar, Shehzad Ali, Kendi Muchungi, Gloria Chemutai, Cynthia Smith, Dominic Trepel, Harris Eyre, Lukoye Atwoli Feb 2025

Life Span Policies And Macroeconomic Transition Will Help The 21st-Century Brain Health Revolution In Developing Countries, Cyprian Mostert, Chinedu Momoh, Manasi Kumar, Shehzad Ali, Kendi Muchungi, Gloria Chemutai, Cynthia Smith, Dominic Trepel, Harris Eyre, Lukoye Atwoli

Brain and Mind Institute

In 2022, the World Health Organization (WHO) issued the Intersectoral Global Action Plan for Epilepsy and Other Neurological Disorders for 2022 to 2031, emphasizing important connections between brain health, population well-being, and economic growth. A year later, the WHO followed up with strategic guidelines aimed at enhancing brain health outcomes in developing countries. However, critical gaps remain. Our policy forum paper advocates for policies that target brain health across all stages of life, starting with measures to reduce the consumption of alcohol, sugar, and tobacco. Additionally, we propose the integration of school meal programs and social pension schemes as essential …


Bayesian Population Pharmacokinetic Modeling Of Ondansetron For Neonatal Opioid Withdrawal Syndrome, Kevin Lam, John Mondick, Gary Peltz, Manhong Wu, Walter Kraft Feb 2025

Bayesian Population Pharmacokinetic Modeling Of Ondansetron For Neonatal Opioid Withdrawal Syndrome, Kevin Lam, John Mondick, Gary Peltz, Manhong Wu, Walter Kraft

Department of Pharmacology, Physiology, and Cancer Biology Faculty Papers

Ondansetron is an anti-emetic 5-HT3 receptor antagonist being investigated for treating neonatal opioid withdrawal syndrome (NOWS). Sparse PK data were analyzed from a multicenter, double-blind clinical trial with 98 mother/neonate dyads. Pregnant women with opioid use disorder were randomized to receive either placebo or ondansetron 8 mg intravenously within 4 h of delivery. Neonates born to mothers who were randomized to ondansetron received 0.07 mg/kg orally once every 24 h for up to five doses. Using current PK data, model parameters from a two-compartmental structural model from the literature (i.e., a priori model) were updated with the Metropolis-Hastings Markov-chain Monte …


Lumpy Heterogeneity In Groundwater Service Values And Time Preferences, Kent F. Kovacs, Heather Snell, Brandon R. Mcfadden, Rodolfo M. Nayga Jr. Feb 2025

Lumpy Heterogeneity In Groundwater Service Values And Time Preferences, Kent F. Kovacs, Heather Snell, Brandon R. Mcfadden, Rodolfo M. Nayga Jr.

Agricultural Economics and Agribusiness Faculty Publications and Presentations

From a choice experiment about groundwater management, we consider time preferences and groundwater service values through sociodemographic characteristics using a model called the discounted latent class model. A policy-motivated latent class has a higher income, greater familiarity with an aquifer, and more concern for the risks that groundwater decline poses to people and the environment. A business-as-usual latent class has a conservative ideology. Exponential discounting is preferred, and the discount rate is larger for the policy group than the business-as-usual group. Other models we use include the discounted mixed logit model and the discounted logit-mixed logit model.


Attenuation Of Vitamin E On Haloxyfop-R-Methyl Ester-Induced Hepatic Oxidative Stress In Orally Exposed Rats., Omolara D. Falodun Feb 2025

Attenuation Of Vitamin E On Haloxyfop-R-Methyl Ester-Induced Hepatic Oxidative Stress In Orally Exposed Rats., Omolara D. Falodun

STEM for Success Resources

No abstract provided.


Determinants Of Market Participation Among Milk Producers In Kyrgyzstan, Akhmadjon Nurullaev, Eunchun Park, Bruce L. Ahrendsen, Stijn Speelman, Lawson Connor Feb 2025

Determinants Of Market Participation Among Milk Producers In Kyrgyzstan, Akhmadjon Nurullaev, Eunchun Park, Bruce L. Ahrendsen, Stijn Speelman, Lawson Connor

Agricultural Economics and Agribusiness Faculty Publications and Presentations

Numerous studies have been conducted to comprehend the economic importance of market participation for producers. However, a significant gap remains, particularly in Central Asia. This study assesses market participation drivers for Kyrgyzstan's milk producers using the Life in Kyrgyzstan dataset, encompassing a comprehensive nationwide farm-level survey. Findings reveal that household assets boost market entry and sales volume, whereas distance from markets discourages participation but can increase sales for active sellers, reflecting transportation cost strategies. The insights inform policy interventions benefiting rural small-scale milk producers in Central Asian countries that share similar cultural backgrounds.


Marine Protected Species Identification Guide 2025, Department Of Primary Industries And Regional Development, Western Australia Feb 2025

Marine Protected Species Identification Guide 2025, Department Of Primary Industries And Regional Development, Western Australia

Fisheries Occasional Publications

The Western Australian aquatic environment is home to a number of species that are protected under international agreements and national or state legislation. Many of these species occur in areas where fishing takes place, sometimes resulting in interactions between fishers and protected species. An interaction is considered to be any physical contact between a person, fishing vessel or fishing gear and a protected species, including captures, entanglements and collisions.

This guide has been developed to help you identify some common marine protected species in Western Australia that you may encounter while fishing.

It has been divided into 5 sections:

• …


Coverage Matters: Identifying And Mitigating Sampling Frame Issues In Recreational Fishing Surveys, Steve Taylor, Annica De Groote, Kieran Hyder, Jon Volstad, B W. Hartill, J Foster, R Andrews, Karina L. Ryan Feb 2025

Coverage Matters: Identifying And Mitigating Sampling Frame Issues In Recreational Fishing Surveys, Steve Taylor, Annica De Groote, Kieran Hyder, Jon Volstad, B W. Hartill, J Foster, R Andrews, Karina L. Ryan

Fisheries Research Articles

Surveys play an integral part in monitoring and maintaining sustainable recreational fisheries. For any probabilistic survey, the selection of a sampling frame (e.g., list of individuals or fishers) is an important decision because it influences the ability to provide unbiased estimates of recreational catch and effort. Undercoverage occurs when units of the target population (i.e., the population of interest) are missing from the frame population. This error can undermine the reliability of research advice generated from survey estimates. In this review, we: (i) define six sampling frame configurations that are commonly applied in probabilistic recreational fishing surveys; (ii) synthesise how …


Gdp-Mannose 4,6-Dehydratase Is A Key Driver Of Mycn-Amplified Neuroblastoma Core Fucosylation And Tumorigenesis, Beibei Zhu, Michelle G. Pitts, Michael D. Buoncristiani, Lindsay T. Bryant, Oscar Lopez-Nunez, Juan P. Gurria, Cameron Shedlock, Roberto Ribas, Shannon Keohane, Jinpeng Liu, Chi Wang, Matthew S. Gentry, Nathan R. Shelman, Derek B. Allison, B. Mark Evers, Ramon C. Sun, Eric J. Rellinger Feb 2025

Gdp-Mannose 4,6-Dehydratase Is A Key Driver Of Mycn-Amplified Neuroblastoma Core Fucosylation And Tumorigenesis, Beibei Zhu, Michelle G. Pitts, Michael D. Buoncristiani, Lindsay T. Bryant, Oscar Lopez-Nunez, Juan P. Gurria, Cameron Shedlock, Roberto Ribas, Shannon Keohane, Jinpeng Liu, Chi Wang, Matthew S. Gentry, Nathan R. Shelman, Derek B. Allison, B. Mark Evers, Ramon C. Sun, Eric J. Rellinger

Markey Cancer Center Faculty Publications

MYCN-amplification is a genetic hallmark of ~40% of high-risk neuroblastomas (NBs). Altered glycosylation is a common feature of adult cancer progression, but little is known about how genetic signatures such as MYCN-amplification alter glycosylation profiles. Herein, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) revealed increased core fucosylated glycan abundance within neuroblast-rich regions of human MYCN-amplified NB tumors. GDP-mannose 4,6-dehydratase (GMDS) is responsible for the first-committed and rate-limiting step of de novo GDP-fucose synthesis. High GMDS expression was found to be associated with poor patient survival, advanced stage disease, and MYCN-amplification in human NB tumors. Chromatin immunoprecipitation and promoter reporter assays …


A Microrna-Regulated Transcriptional State Defines Intratumoral Cd8+ T Cells That Respond To Immunotherapy, William W. Tang, Ben Battistone, Kaylyn M. Bauer, Allison M. Weis, Cindy Barba, Muhammad Zaki Hidayatullah Fadlullah, Arevik Ghazaryan, Van B. Tran, Soh-Hyun Lee, Z. Busra Agir, Morgan C. Nelson, Emmanuel Stephen Victor, Amber Thibeaux, Colton Hernandez, Jacob Tantalla, Aik C. Tan, Dinesh Rao, Matthew Williams, Micah J. Drummond, Ellen J. Beswick, June L. Round, H. Atakan Ekiz, Warren P/ Voth, Ryan M. O’Connell Feb 2025

A Microrna-Regulated Transcriptional State Defines Intratumoral Cd8+ T Cells That Respond To Immunotherapy, William W. Tang, Ben Battistone, Kaylyn M. Bauer, Allison M. Weis, Cindy Barba, Muhammad Zaki Hidayatullah Fadlullah, Arevik Ghazaryan, Van B. Tran, Soh-Hyun Lee, Z. Busra Agir, Morgan C. Nelson, Emmanuel Stephen Victor, Amber Thibeaux, Colton Hernandez, Jacob Tantalla, Aik C. Tan, Dinesh Rao, Matthew Williams, Micah J. Drummond, Ellen J. Beswick, June L. Round, H. Atakan Ekiz, Warren P/ Voth, Ryan M. O’Connell

Markey Cancer Center Faculty Publications

The rising incidence of advanced-stage colorectal cancer (CRC) and poor survival outcomes necessitate new and effective therapies. Immune checkpoint inhibitors (ICIs), specifically anti-PD-1 therapy, show promise, yet clinical determinants of a positive response are suboptimal. Here, we identify microRNA-155 (miR-155) as necessary for CD8 + T cell-infiltrated tumors through an unbiased in vivo CRISPR-Cas9 screen identifying functional tumor antigen-specific CD8+ T cell-expressed microRNAs. T cell miR-155 is required for anti-PD-1 responses and for a vital intratumor CD8 + T cell differentiation cascade by repressing Ship-1, inhibiting Tcf-1 and stemness, and subsequently enhancing Cxcr6 expression, anti-tumor immunity, and effector functions. Based …


Staying Sane In The Membrane: Neutral Sphingomyelinase 2 As A Master Regulator Of Plasma Membrane Ceramide, Zainuddin Quadri, Erhard Bieberich Feb 2025

Staying Sane In The Membrane: Neutral Sphingomyelinase 2 As A Master Regulator Of Plasma Membrane Ceramide, Zainuddin Quadri, Erhard Bieberich

Markey Cancer Center Faculty Publications

Ceramide, a key signaling sphingolipid in the plasma membrane, plays a pivotal role in fundamental cellular processes such as adhesion, polarity, and programmed cell death. The generation of plasma membrane ceramide is largely attributed to the activity of two types of sphingomyelinases: neutral sphingomyelinase 2 (nSMase2, Smpd3) and acid sphingomyelinase (aSMase, Smpd1). While many studies have explored ceramide generation following experimental activation of these enzymes, the mechanisms governing basal or steady- state ceramide levels have remained poorly understood. Using an innovative mass spectrometry approach developed in the Canals’ lab, the team has quantified the distinct contributions of nSMase2 and aSMase …


Bilirubin Bioconversion To Urobilin In The Gut-Liver-Kidney Axis: A Biomarker For Insulin Resistance In The Cardiovascular-Kidney-Metabolic (Ckm) Syndrome, Zachary A. Kipp, Olufunto O. Badmus, David E. Stec, Brantley Hall, Terry D. Hinds Jr. Feb 2025

Bilirubin Bioconversion To Urobilin In The Gut-Liver-Kidney Axis: A Biomarker For Insulin Resistance In The Cardiovascular-Kidney-Metabolic (Ckm) Syndrome, Zachary A. Kipp, Olufunto O. Badmus, David E. Stec, Brantley Hall, Terry D. Hinds Jr.

Markey Cancer Center Faculty Publications

The rising rates of obesity worldwide have increased the incidence of cardiovascular disease (CVD), making it the number one cause of death. Higher plasma bilirubin levels have been shown to prevent metabolic dysfunction and CVD. However, reducing levels leads to deleterious outcomes, possibly due to reduced bilirubin half-life that escalates the production of its catabolized product, urobilinogen, produced by gut bacteria and naturally oxidized to urobilin. Recent findings suggest that the involvement of the microbiome catabolism of bilirubin to urobilin and its absorption via the hepatic portal vein contributes to CVD, suggesting a liver-gut axis involvement. We discuss the studies …


Ddo-Adjuvanted Influenza A Virus Nucleoprotein Mrna Vaccine Induces Robust Humoral And Cellular Type 1 Immune Responses And Protects Mice From Challenge, Victoria Gnazzo, Hanaa Saleh, Ítalo A. Castro, Adrianus C. M. Boon, Amelia K. Pinto, James D. Brien, Carolina B. López Feb 2025

Ddo-Adjuvanted Influenza A Virus Nucleoprotein Mrna Vaccine Induces Robust Humoral And Cellular Type 1 Immune Responses And Protects Mice From Challenge, Victoria Gnazzo, Hanaa Saleh, Ítalo A. Castro, Adrianus C. M. Boon, Amelia K. Pinto, James D. Brien, Carolina B. López

Markey Cancer Center Faculty Publications

A challenge in viral vaccine development is to produce vaccines that generate both neutralizing antibodies to prevent infection and cytotoxic CD8+ T-cells that target conserved viral proteins and can eliminate infected cells to control virus spread. mRNA technology offers an opportunity to design vaccines based on conserved CD8-targeting epitopes, but achieving robust antigen-specific CD8+ T-cells remains a challenge. Here, we tested the viral-derived oligonucleotide DDO268 as an adjuvant in the context of a model influenza A virus (IAV) nucleoprotein (NP) mRNA vaccine in C57BL/6 mice. DDO268 when co-packaged with mRNA in lipid nanoparticles is sensed by RIG I-like receptors and …


Gdp-Mannose 4,6-Dehydratase Is A Key Driver Of Mycn- Amplified Neuroblastoma Core Fucosylation And Tumorigenesis, Beibei Zhu, Michelle G. Pitts, Michael D. Buoncristiani, Lindsay T. Bryant, Oscar Lopez-Nunez, Juan P. Gurria, Cameron Shedlock, Roberto Ribas, Shannon Keohane, Jinpeng Liu, Chi Wang, Matthew S. Gentry, Nathan R. Shelman, Derek B. Allison, B. Mark Evers, Ramon C. Sun, Eric J. Rellinger Feb 2025

Gdp-Mannose 4,6-Dehydratase Is A Key Driver Of Mycn- Amplified Neuroblastoma Core Fucosylation And Tumorigenesis, Beibei Zhu, Michelle G. Pitts, Michael D. Buoncristiani, Lindsay T. Bryant, Oscar Lopez-Nunez, Juan P. Gurria, Cameron Shedlock, Roberto Ribas, Shannon Keohane, Jinpeng Liu, Chi Wang, Matthew S. Gentry, Nathan R. Shelman, Derek B. Allison, B. Mark Evers, Ramon C. Sun, Eric J. Rellinger

Markey Cancer Center Faculty Publications

MYCN-amplification is a genetic hallmark of ~40% of high-risk neuroblastomas (NBs). Altered glycosylation is a common feature of adult cancer progression, but little is known about how genetic signatures such as MYCN-amplification alter glycosylation profiles. Herein, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) revealed increased core fucosylated glycan abundance within neuroblast-rich regions of human MYCN-amplified NB tumors. GDP-mannose 4,6-dehydratase (GMDS) is responsible for the first-committed and rate-limiting step of de novo GDP-fucose synthesis. High GMDS expression was found to be associated with poor patient survival, advanced stage disease, and MYCN-amplification in human NB tumors. Chromatin immunoprecipitation and promoter reporter assays …


Evaluating The Generalisability Of Region-Naïve Machine Learning Algorithms For The Identification Of Epilepsy In Low-Resource Settings, Ioana Duta, Symon M. Kariuki, Anthony Ngugi, Angelina Kakooza Mwesige, Honorati Masanja, Daniel M. Mwanga, Seth Owusu-Agyei, Ryan Wagner, J Helen Cross, Josemir W. Sander W. Sander Feb 2025

Evaluating The Generalisability Of Region-Naïve Machine Learning Algorithms For The Identification Of Epilepsy In Low-Resource Settings, Ioana Duta, Symon M. Kariuki, Anthony Ngugi, Angelina Kakooza Mwesige, Honorati Masanja, Daniel M. Mwanga, Seth Owusu-Agyei, Ryan Wagner, J Helen Cross, Josemir W. Sander W. Sander

Population Health, East Africa

Objectives: Approximately 80% of people with epilepsy live in low- and middle-income countries (LMICs), where limited resources and stigma hinder accurate diagnosis and treatment. Clinical machine learning models have demonstrated substantial promise in supporting the diagnostic process in LMICs by aiding in preliminary screening and detection of possible epilepsy cases without relying on specialised or trained personnel. How well these models generalise to naïve regions is, however, underexplored. Here, we use a novel approach to assess the suitability and applicability of such clinical tools to aid screening and diagnosis of active convulsive epilepsy in settings beyond their original training contexts. …


National Ffa Parliamentary Procedure And Conduct Of Chapter Meetings Leadership Development Events Presentation Generator, P. Troy White Feb 2025

National Ffa Parliamentary Procedure And Conduct Of Chapter Meetings Leadership Development Events Presentation Generator, P. Troy White

Department of Agricultural Leadership, Education, and Communication: Faculty Publications

A Microsoft Excel macro-enabled workbook that produces presentation cards for the National FFA Organization's Parliamentary Procedure and Conduct of Chapter Meetings Leadership Development Events.

Each set of cards also creates a judges help sheet for the motions with proper verbiage and the Standard Descriptive Characteristics as listed in Robert's Rules of Order Newly Revised (12th edition).

The workbook uses randomization to create set cards from a list of over 300 possible main motions from a variety of internet sources. The 2024 list of eligible motions for both events was used for the permissible motions.


Brain Transcriptomics Of A Social Challenge And Maternal Aggression In Incubating Female Tree Swallows, Emily J. Levy, Elizabeth M. George, Douglas B. Rusch, Aaron Buechlein, Kimberly A. Rosvall Feb 2025

Brain Transcriptomics Of A Social Challenge And Maternal Aggression In Incubating Female Tree Swallows, Emily J. Levy, Elizabeth M. George, Douglas B. Rusch, Aaron Buechlein, Kimberly A. Rosvall

Biology Faculty Publications

Aggressive behavior is ubiquitous across many contexts, including defense of territories, mates, and offspring. For decades, researchers have detailed the effect of aggressive behavior on physiology, but our understanding of these mechanisms in females lags behind that of males, despite the fact that female aggression is widespread, particularly in the context of maternal defense of eggs or offspring (i.e., maternal aggression). Here, we measured effects of a social challenge on brain gene expression in free-living incubating females. We hypothesized that the social challenge would generate at least one of three transcriptomic effects: (1) sensitizing the brain to otherwise low levels …


Host Traits And Temperature Predict Biogeographical Variation In Seagrass Disease Prevalence, F.R. Schenck, J.K. Baum, K.E. Boyer, J.E. Duffy, F.J. Fodrie, J. Gaeckle, Torrie Hanley, C.M. Hereu, K.A. Hovel, P. Jorgensen, D.L. Martin, N.E. O’Connor, B.J. Peterson, J.J. Stachowicz, A.R. Hughes Feb 2025

Host Traits And Temperature Predict Biogeographical Variation In Seagrass Disease Prevalence, F.R. Schenck, J.K. Baum, K.E. Boyer, J.E. Duffy, F.J. Fodrie, J. Gaeckle, Torrie Hanley, C.M. Hereu, K.A. Hovel, P. Jorgensen, D.L. Martin, N.E. O’Connor, B.J. Peterson, J.J. Stachowicz, A.R. Hughes

Biology Faculty Publications

Diseases are ubiquitous in natural systems, with broad effects across populations, communities and ecosystems. However, the drivers of many diseases remain poorly understood, particularly in marine environments, inhibiting effective conservation and management measures. We examined biogeographical patterns of infection in the foundational seagrass Zostera marina by the parasitic protist Labyrinthula zosterae, the causative agent of seagrass wasting disease, across >20° of latitude in two ocean basins. We then identified and characterized relationships among wasting disease prevalence and a suite of host traits and environmental variables. Host characteristics and transmission dynamics explained most of the variance in prevalence across our survey, …


Factors Influencing The Adoption Of Agroecological Vegetable Cropping Systems By Smallholder Farmers In Tanzania, Essy C. Kirui, Michael M. Kidoido, Komivi S. Akutse, Rosina Wanyama, Simon B. Boni, Thomas Dubois, Fekadu F. Dinssa, Daniel M. Mutyambai Feb 2025

Factors Influencing The Adoption Of Agroecological Vegetable Cropping Systems By Smallholder Farmers In Tanzania, Essy C. Kirui, Michael M. Kidoido, Komivi S. Akutse, Rosina Wanyama, Simon B. Boni, Thomas Dubois, Fekadu F. Dinssa, Daniel M. Mutyambai

All Peer-Reviewed Publications

Vegetable production is vital to smallholder farmers, who often struggle to overcome pests, diseases, and extreme weather. Agroecological cropping systems offer sustainable solutions to these issues but their adoption rates in Tanzania remain low. This study examines the factors influencing smallholder farmers’ adoption of selected agroecological cropping systems for vegetable production in Tanzania, which remains underexplored. Using a multistage sampling technique, cross-sectional data were gathered from 525 crucifer and traditional African vegetable farming households within the Arusha and Kilimanjaro regions. Multivariate probit regression analysis, which accounts for the simultaneous adoption of multiple systems, revealed several significant variables influencing adoption. The …


Climate Effects On Honey Bees Can Be Mitigated By Beekeeping Management In Kenya, Malena Sibaja Leyton, H. Michael G. Lattorff, Nkoba Kiatoko, Fabrice Requier Feb 2025

Climate Effects On Honey Bees Can Be Mitigated By Beekeeping Management In Kenya, Malena Sibaja Leyton, H. Michael G. Lattorff, Nkoba Kiatoko, Fabrice Requier

All Peer-Reviewed Publications

In recent decades, worldwide concerns about the health of honey bees motivated the development of surveys to monitor the colony losses, of which Sub-Saharan Africa has had limited representation. In the context of climate change, understanding how climate affects colony losses has become fundamental, yet literature on this subject is scarce. For the first time, we conducted a survey to estimate the livestock decrease of honey bee colonies in Kenya for the year 2021–2022 to explore the effects of environmental conditions, such as temperature and precipitation, on livestock decrease. We define “livestock decrease” from the beekeeper's perspective, including dead colonies …