Life Sciences Commons^™

Sewing With Fur, Cory Farnsworth

All Current Publications

Today, trapping is one of the most regulated activities worldwide and is a useful tool in managing excess populations of furbearers and struggling populations of prey animals. Fur is slowly regaining popularity as people recognize it as more ethical, humane, economical, and renewable, but the ability to use fur is becoming a lost art. It is part of our heritage as a people and connects us with the land. Learning to sew fur projects can be fun and, in some cases, quite profitable, as fur sewn into an article will sell far above the price of the pelt itself. If …

(Winter Skate (Leucoraja Ocellata)) Jaw, Michael A. Birnbaum, David Kerstetter

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

No abstract provided.

The Lc3-Interacting Region Of Nbr1 Is A Protein Interaction Hub Enabling Optimal Flux, Brian Joseph North

Dartmouth College Ph.D Dissertations

Autophagy is a highly conserved pathway that maintains cell health by breaking down and recycling cellular components such as protein aggregates or damaged organelles. During autophagy, potentially toxic cargo is enveloped by a newly formed autophagosome and trafficked to the lysosome for degradation. Ubiquitinated protein aggregates, a key target for autophagy, are identified by multiple autophagy receptors. NBR1 is an archetypal autophagy receptor and an excellent model for deciphering the role of the multivalent, heterotypic interactions made by cargo-bound receptors. Using NBR1 as a model, we find that three critical binding partners—ATG8-family proteins, FIP200, and TAX1BP1—each bind to a short …

A Barley Pan-Transcriptome Reveals Layers Of Genotype-Dependent Transcriptional Complexity, Wenbin Guo, Miriam Schreiber, Vanda B. Marosi, Paolo Bagnaresi, Morten Egevang Jorgensen, Katarzyna B. Braune, Ken J. Chalmers, Brett Chapman, Viet Hoang Dang, Christoph Dockter, Anne Fiebig, Geoffrey B. Fincher, Agostino Fricano, John Fuller, Allison Haaning, Georg Haberer, Axel Himmelbach, Murukarthick Jayakodi, Yong Jia, Nadia Kamal, Peter Langridge, Chengdao Li

Grain and Other Field Crops Research Articles

A pan-transcriptome describes the transcriptional and post-transcriptional consequences of genome diversity from multiple individuals within a species. We developed a barley pan-transcriptome using 20 inbred genotypes representing domesticated barley diversity by generating and analyzing short- and long-read RNA-sequencing datasets from multiple tissues. To overcome single reference bias in transcript quantification, we constructed genotype-specific reference transcript datasets (RTDs) and integrated these into a linear pan-genome framework to create a pan-RTD, allowing transcript categorization as core, shell or cloud. Focusing on the core (expressed in all genotypes), we observed significant transcript abundance variation among tissues and between genotypes driven partly by RNA …

Distinct Roles Of Mtorc2 In Excitatory And Inhibitory Neurons In Inflammatory And Neuropathic Pain, Wei He, Xin Ge, Ru-Rong Ji

Faculty, Staff and Student Publications

No abstract provided.

Understanding Pediatric Bipolar Disorder Through The Investigation Of Clinical, Neuroanatomic, Neurophysiological And Neurocognitive Dimensions: A Pilot Study, Alessio Simonetti, Evelina Bernardi, Sherin Kurian, Antonio Restaino, Claudia Calderoni, Emanuela De Chiara, Francesca Bardi, Gabriele Sani, Jair C Soares, Kirti Saxena

Faculty, Staff and Student Publications

Background: Pathophysiological models of pediatric bipolar disorder (PBD) are lacking. Multimodal approaches may provide a comprehensive description of the complex relationship between the brain and behavior. Aim: To assess behavioral, neuropsychological, neurophysiological, and neuroanatomical alterations in youth with PBD.

Methods: Subjects with PBD (n = 23) and healthy controls (HCs, n = 23) underwent (a) clinical assessments encompassing the severity of psychiatric symptoms, (b) neuropsychological evaluation, (c) analyses of event-related potentials (related to the passive viewing of fearful, neutral, and happy faces during electroencephalography recording, and (d) cortical thickness and deep gray matter volume measurement using magnetic …

Deescalation Perils In Inflammatory Breast Cancer, Wendy A Woodward

Faculty, Staff and Student Publications

No abstract provided.

Phase I/Ii Study Of The Aurora Kinase A Inhibitor Alisertib And Pembrolizumab In Refractory, Rb-Deficient Head And Neck Squamous Cell Carcinomas, Faye M Johnson, Madison P O'Hara, Lacin Yapindi, Peixin Jiang, Hai T Tran, Alexandre Reuben, Weihong Xiao, Maura L Gillison, Xiaowen Sun, Alexander Khalaf, J Jack Lee, Jagannadha K Sastry, Soma Ghosh

Faculty, Staff and Student Publications

Purpose: Effective therapy for recurrent head and neck squamous cell carcinoma (HNSCC) that is refractory to chemotherapy and immunotherapy is a considerable need. Aurora kinase A inhibition leads to apoptosis and immunogenic cell death in preclinical models of human papilloma virus (HPV)-driven cancers.

Patients and methods: Alisertib was administered orally twice daily on days 1-7 and pembrolizumab on day 1 of a 21-day cycle to adults with advanced solid tumors (phase I) or with immunotherapy- and platinum-resistant, HPV-positive HNSCC (phase II).

Results: The recommended phase II alisertib dose was 40 mg, which had only the expected toxicity including cytopenia that …

The Landscape Of Ctdna In Appendiceal Adenocarcinoma, Michael G White, Mohammad A Zeineddine, Eleanor A Fallon, Fadl A Zeineddine, Julia Dansby, Saikat Chowdhury, Nicholas Hornstein, Abdelrahman Yousef, Mahmoud Yousef, Neal Bhutiani, Yue Gu, Bryan Kee, Arvind Dasari, Michael J Overman, Kanwal Raghav, Scott Kopetz, Abhineet Uppal, Melissa Taggart, Timothy Newhook, Keith Fournier, Beth Helmink, Leylah M Drusbosky, John Paul Shen

Faculty, Staff and Student Publications

Purpose: Appendiceal adenocarcinoma is a rare malignancy with distinct histopathologic subtypes and a natural history with metastasis primarily limited to the peritoneum. Little is known about the molecular pathogenesis of appendiceal adenocarcinoma relative to common tumors.

Experimental design: We analyzed molecular data for patients within the Guardant Health database with appendix cancer (n = 718). We then identified patients with appendiceal adenocarcinoma at our institution (from October 2004-September 2022) for whom ctDNA mutation profiling (liquid biopsy) was performed (n = 168) and extracted clinicopathologic and outcomes data. Of these 168 patients, 57 also had tissue-based tumor mutational profiling, allowing for …

Antitumor Activity And Biomarker Analysis For Trop2 Antibody-Drug Conjugate Datopotamab Deruxtecan In Patient-Derived Breast Cancer Xenograft Models, Funda Meric-Bernstam, Erkan Yuca, Kurt W Evans, Ming Zhao, Takanori Maejima, Tsuyoshi Karibe, Maria Gabriela Raso, Ximing Tang, Xiaofeng Zheng, Yasmeen Qamar Rizvi, Argun Akcakanat, Stephen M Scott, Bailiang Wang, Lauren A Byers, Debu Tripathy, Daisuke Okajima, Senthil Damodaran

Faculty, Staff and Student Publications

PURPOSE: Datopotamab deruxtecan (Dato-DXd) is a humanized anti-trophoblast cell-surface antigen-2 (TROP2) IgG1 mAb linked to a potent topoisomerase I inhibitor payload (DXd). Dato-DXd has already shown antitumor activity in breast cancer; however, the determinants of response, including the importance of TROP2 expression, remain unclear. We tested the activity of Dato-DXd in a panel of breast cancer patient-derived xenografts (BCX) varying in TROP2 expression.

EXPERIMENTAL DESIGN: The antitumor activity of Dato-DXd and isotype-control-DXd (IgG-DXd) was assessed against 11 BCXs varying in TROP2 expression, 10 representing tumors postneoadjuvant chemotherapy. Pharmacodynamic effects were assessed at 24 and 72 hours. The effects of TROP2 …

Early Tolerance And Late Persistence As Alternative Drug Responses In Cancer, Simona Punzi, Davide Cittaro, Guido Gatti, Gemma Crupi, Oronza A Botrugno, Antonino Alex Cartalemi, Alon Gutfreund, Caterina Oneto, Valentina Giansanti, Chiara Battistini, Giovanni Santacatterina, Lucrezia Patruno, Ilaria Villanti, Martina Palumbo, Daniel J Laverty, Francesca Giannese, Alex Graudenzi, Giulio Caravagna, Marco Antoniotti, Zachary Nagel, Ugo Cavallaro, Luisa Lanfrancone, Timothy A Yap, Giulio Draetta, Nathalie Balaban, Giovanni Tonon

Faculty, Staff and Student Publications

Bacteria withstand antibiotic treatment through three alternative mechanisms: resistance, persistence or tolerance. While resistance and persistence have been described, whether drug-induced tolerance exists in cancer cells remains largely unknown. Here, we show that human cancer cells elicit a tolerant response when exposed to commonly used chemotherapy regimens, propelled by the pervasive activation of autophagy, leading to the comprehensive activation of DNA damage repair pathways. After prolonged drug exposure, such tolerant responses morph into persistence, whereby the increased DNA damage repair is entirely reversed. The central regulator of mitophagy PINK1 drives this reduction in DNA repair via the cytoplasmic relocalization of …

Plant-Nanoparticles Enhance Anti-Pd-L1 Efficacy By Shaping Human Commensal Microbiota Metabolites, Yun Teng, Chao Luo, Xiaolan Qiu, Jingyao Mu, Mukesh K Sriwastva, Qingbo Xu, Minmin Liu, Xin Hu, Fangyi Xu, Lifeng Zhang, Juw Won Park, Jae Yeon Hwang, Maiying Kong, Zhanxu Liu, Xiang Zhang, Raobo Xu, Jun Yan, Michael L Merchant, Craig J Mcclain, Huang-Ge Zhang

Faculty, Staff and Student Publications

Diet has emerged as a key impact factor for gut microbiota function. However, the complexity of dietary components makes it difficult to predict specific outcomes. Here we investigate the impact of plant-derived nanoparticles (PNP) on gut microbiota and metabolites in context of cancer immunotherapy with the humanized gnotobiotic mouse model. Specifically, we show that ginger-derived exosome-like nanoparticle (GELN) preferentially taken up by Lachnospiraceae and Lactobacillaceae mediated by digalactosyldiacylglycerol (DGDG) and glycine, respectively. We further demonstrate that GELN aly-miR159a-3p enhances anti-PD-L1 therapy in melanoma by inhibiting the expression of recipient bacterial phospholipase C (PLC) and increases the accumulation of docosahexaenoic acid …

Safety And Tolerability Of Letetresgene Autoleucel (Gsk3377794): Pilot Studies In Patients With Advanced Non-Small Cell Lung Cancer, Mehmet Altan, Gilberto Lopes, T Jeroen N Hiltermann, Ramaswamy Govindan, Liza C Villaruz, Emiliano Calvo, Martin J Edelman, Muhammad Furqan, Joel Neal, Enriqueta Felip, Jennifer W Carlisle, John V Heymach, Róisín Eilish O'Cearbhaill, Marjorie Zauderer, Michael Chisamore, Ellie Corigliano, Ioanna Eleftheriadou, Stefan Zajic, Ben Jenkins, Sophia Goodison, Sunil Suchindran, Natalia Ramos-Hernandez, Nidale Tarek, Adam J Schoenfeld

Faculty, Staff and Student Publications

Purpose: The study aims to evaluate the safety, tolerability, and antitumor response of letetresgene autoleucel (lete-cel), genetically modified autologous T cells expressing a T-cell receptor specific for New York esophageal squamous cell carcinoma 1 (NY-ESO-1)/LAGE-1a shared epitope, alone or in combination with pembrolizumab, in HLA-A*02-positive (HLA-A*02:01, HLA-A*02:05, and/or HLA-A*02:06) patients with NY-ESO-1- and/or LAGE-1a-positive non-small cell lung cancer.

Patients and methods: Study 208749 was a single-arm study of lete-cel alone. Study 208471 was a multiarm study of lete-cel alone or in combination with pembrolizumab in patients with advanced or recurrent non-small cell lung cancer.

Results: More than 2,500 patients were …

Exploring Single Cell Microbial Protein As A Sustainable Fishmeal Alternative In Yellowtail Kingfish (Seriola Lalandi) Diets: Impacts On Health And Gut Microbiome, Luke Pilmer, Lindsey Woolley, Alan Lymbery, Chinh Dam, Abigail Elizur, Md Javed Foysal, Gavin Partridge

Fisheries Research Articles

Background

With the global expansion of aquaculture and the increasing demand for fish meal, identifying appropriate and sustainable alternative protein sources for aquafeeds has become essential. Single-cell protein (SCP), derived from methanotrophic bacteria, presents a promising alternative by converting methane into protein, potentially addressing both the need for alternative protein sources and reducing industrial greenhouse gas emissions. This study aimed to evaluate the effects of different levels of SCP inclusion (0%, 25%, 50%, and 75% fish meal replacement) on the health, gene expression, and gut microbiome of yellowtail kingfish (YTK, Seriola lalandi) following a 35-day growth trial.

Results

The …

Impacts Of Changing Temperatures On The Water Budget In The Great Salt Lake Basin, Grace Affram, Jihad Othman, Reza Morovati, Saddy Pineda Castellanos, Sajad Khoshnoodmotlagh, Diana Dunn, Braedon Dority, Katherine Osorio Diaz, Cody Ratterman, Wei Zhang

Plants, Soils and Climate Student Research

Quantifying the water budget in the Great Salt Lake (GSL) basin is a nontrivial task, especially under a changing climate that contributes to increasing temperatures and a shift towards more rainfall and less snowfall. This study examines the potential impacts of temperature thresholds on the water budget in the GSL, emphasizing the influence on snowmelt, evapotranspiration (ET), and runoff under varying climate warming scenarios. Current hydrological models such as the Variable Infiltration Capacity (VIC) model use a universal temperature threshold to partition snowfall and rainfall across different regions. Previous studies have argued that there is a wide range of thresholds …

Defining The Role Of Adjuvant Radiotherapy For Biliary Tract Cancers: A Site-Specific Propensity-Matched Analysis, Yongwoo David Seo, Belkacem Acidi, Andrew Newton, Antony Haddad, Yi-Ju Chiang, Rainna Coelho, Timothy E Newhook, Ching-Wei D Tzeng, Yun Shin Chun, Ethan B Ludmir, Eugene J Koay, Milind Javle, Jean Nicolas Vauthey, Hop S Tran Cao

Faculty, Staff and Student Publications

Background: Biliary tract cancers (BTCs) have distinct tumor biology but share a poor prognosis, with a 5-year-survival-rate of 5-19%. Surgical resection is the only potential cure, but recurrences are common. The role of adjuvant radiotherapy (XRT) remains unclear.

Methods: Using the National Cancer Database (2006-2018), we analyzed resected non-metastatic BTCs. Patients who survived beyond 90 days post-surgery were included, while those with R2 resections or neoadjuvant therapy were excluded. Propensity matching was performed based on predictors of adjuvant radiation, age, and sex. Survival outcomes were compared between no adjuvant therapy, chemotherapy alone, and XRT ± chemotherapy.

Results: …

Developing Sustainable Medical Tourism: A Framework In The Perspective Of Developing Countries, Abel Ansporthy Mamboleo Dr.

Journal of Sustainability and Resilience

This study reviewed the literature to develop the framework for sustainable medical tourism in developing countries. According to the findings, the development of a sustainable tourist destination necessitates the involvement of various stakeholders and adherence to sustainability principles. The framework suggests five essential tasks for sustainable medical tourism development. It contributes to the knowledge of sustainable medical tourism by suggesting the framework for analysing a specific situation for the development of a medical tourist destination. It provides a better understanding of sustainable medical tourism operations by integrating medication, travel, hospitality, and tourism. Moreover, it contributes to the sphere of medical …

Woody Plant Reinvasion Shortens The Lifespan Of Grassland Restoration Treatments, Dillon T. Fogarty, Michele Beadle, Craig R. Allen, Christine Bielski, Dirac Twidwell

Biology Student Work

An important question in restoration ecology is whether restored ecological regimes are more vulnerable to transitions back to a degraded state. In woody-invaded grasslands, high-intensity fire can collapse woody plant communities and induce a shift back to a grass-dominated regime. Yet, legacies from woody-dominated regimes often persist and it remains unclear whether restored regimes are at heightened vulnerability to reinvasion. In this study, we utilize a 17-year history of fire-based restoration in Nebraska's Loess Canyons Experimental Landscape to determine whether restored grassland regimes experience faster rates of Juniperus virginiana (eastern redcedar) reinvasion compared to the initial invasion process in adjacent …

Effects Of Initial Foot Position On Neuromuscular And Biomechanical Control During The Stand-To-Sit Movement: Implications For Rehabilitation Strategies, Woohyoung Jeon, Ashley Dalby, Xuanliang Neil Dong, Chung-Hyun Goh

Health & Kinesiology Faculty Publications and Presentations

Background: Initial foot position (IFP) is one of the important movement strategies that influence neuromuscular and biomechanical control during sit-to-stand (STS) movements. Similarly, stand-to-sit (StandTS) is vital in rehabilitation settings for evaluating strength and balance control during descending movements. Understanding how IFP impacts changes in biomechanical and neuromuscular movement control factors during StandTS can provide valuable insights for designing effective rehabilitation programs.

Methods: Twelve healthy young adults participated in this study, examining three symmetric IFPs: (1) REF (reference); (2) wide: each foot was shifted outwards by 30% from REF; (3) TO (toes-out): symmetric toes-out angle of 30° from REF. Kinematic …

Icipe Monthly Digest – Volume 1, Issue 2, February 2025, International Centre Of Insect Physiology And Ecology (Icipe)

icipe Newsletters

No abstract provided.

Attitudes Toward The Continued Protection Of Grizzly Bears Under The Endangered Species Act, John A. Vucetich, Jeremy T. Bruskotter

Michigan Tech Publications

In January 2025, the U.S. Fish & Wildlife Service published a proposed rule to continue protecting grizzly bears in the contiguous United States under the Endangered Species Act (ESA). The proposed rule and guidance for how the public can comment on the proposed rule can be found at this link: https://www.fws.gov/press-release/2025-01/usfws-proposes-update-grizzly-bear-esa-listing-management

In January 2025, we implemented a web-based survey designed to assess the attitudes of residents of the contiguous United States, as they pertain to the appropriateness of continuing protection of grizzly bears under the ESA. A large body of prior research indicates that individuals’ attitudes toward species protections can …

Operationalizing And Measuring Climate Change Adaptation Success, Henry A. Bartelet, Michele L. Barnes, Lalu A.A. Bakti, Graeme S. Cumming

Quantitative Methods and Information Technology Faculty Publications

In a context of rapid global change, understanding whether and how adaptation to climate change can be considered successful has become an important research gap within the climate change adaptation literature. Although definitions of adaptation success have been formulated, it remains unclear how they can be operationalized and tested empirically. To address this gap, we operationalized one of the most prominent definitions of successful adaptation within the academic literature, which describes success as adaptations that support reductions in risk and vulnerability without compromising sustainability. Specifically, drawing on data collected from 209 coral reef tourism operators across 28 locations and eight …

3d Printing Insect Models For K-12 Education, Henry L. Whittemore, Jason Oliver, Karla M. Addesso

Extension Publications

No abstract provided.

An Approach To Estimate Angling Effort, Catch, And Harvest Of Burbot Lota Lota, Collin J. Mitchell

Biology Graduate Theses

Once commonly thought of as a nuisance, Burbot Lota lota are now being frequently targeted by anglers in Minnesota. However, the amount of angling effort directed at Burbot, the distribution of effort, catch, and harvest are unknown. Historically, creel surveys have been used to estimate angling effort, catch, and harvest. Additionally, digital cameras have been used independently and supplementally to estimate angling effort, catch, and harvest. In this study, we estimate angling effort, catch, and harvest of Burbot using creel survey techniques and motion-activated digital cameras on Bad Medicine Lake, Minnesota, while also investigating and exploring the limitations of these …

Tremblings, February 2025, Western Aspen Alliance

Tremblings

Bridging Conservation Science With Native Meaning

James Calabaza, Indigenous Lands Director; Trees, Water & People

Last fall, I had the privilege of collaborating with the Western Aspen Alliance and other partners to carry out a 3-day workshop focused on quaking aspen genetics, fire ecology, and co-stewardship. Held at Rocky Mountain Biological Laboratory (Gothic, Colorado), the workshop brought together academics, NGOs, federal agencies, and Tribal members to share diverse perspectives on aspen's significance and management futures.

Life Among The Sand Grains, Will M. Ballentine, N. G. Roberts, M. K. Yap-Chiongco, K. M. Halanych, K. M. Kocot

Biology Faculty Works

Have you ever played with sand at the beach? Did you know you were also playing with thousands of tiny animals? The sand in the ocean is home to many small creatures called meiofauna, but you can only see them under a magnifying glass or microscope. Meiofauna are so tiny that sand grains seem like boulders to them. Meiofauna are too small to move these boulders, so they wriggle through spaces between them. Living in such tight spaces has led them to evolve long, skinny body shapes like worms. They also have special body parts to grip sand grains and …

Conformation And Membrane Topology Of The N-Terminal Ectodomain Of Influenza A M2 Protein, Kyra C. Roepke , '24, Kathleen P. Howard

Chemistry & Biochemistry Faculty Works

The N-terminal ectodomain of the influenza A M2 protein is a target for universal influenza vaccine development and novel antiviral strategies. Despite the significance of this domain, it is poorly understood and most structural studies of the M2 protein have disregarded the N-terminal ectodomain in their analyses. Here, we report conformational properties and describe insights into the membrane topology of sites along the N-terminal ectodomain. Full-length M2 protein is embedded in lipid bilayer nanodiscs and studied using site-directed spin labeling electron paramagnetic resonance spectroscopy. Results are consistent with a turn in the middle of the ectodomain that changes in proximity …

Development Of The Navigation Guide Evidence-To-Decision Framework For Environmental Health: Version 1.0, Nicholas Chartres, Max T. Aung, Sustan L. Norris, Courtney Cooper, Lia A. Bero, Roger Chou, Devon C. Payne-Sturges, Wendy E. Wagner, Jessica W. Reyes, Lisa M. Askie, Daniel A. Axelrad, Deysi Flores Vigo, Jill E. Johnston, Juleen Lam, Keeve E. Nachman, Eva Rehfuess, Rachel Rothschild, Patrice Sutton, Lauren Zeise, Tracey J. Woodruff

Articles

Environmental exposures, including widespread industrial pollution, impact human health and are amplified in more highly exposed communities. Policy and regulatory frameworks for making decisions and recommendations on interventions to mitigate or prevent exposures tend to narrowly focus on exposure and some health-related data related to risks. Typically, such frameworks do not consider other factors, including essentiality, health equity, and distribution of benefits and costs. Further, decisions and recommendations lack transparency regarding how they were developed. We developed the Navigation Guide Evidence-to-Decision Framework for Environmental Health (E2DFEH) to provide a structured and transparent framework incorporating a range of scientific information and …

Higher Amounts Of Habitual Physical Activity Changes The Relationship Between Hot Flashes And Subclinical Cardiovascular Disease Risk, Sarah Witkowski, Tint Tha Ra Wun, Jo Sophia Brunzelle, Sara Buszkiewicz, Lorna Murphy, Randi L. Garcia, Lynnette Leidy Sievert

Exercise and Sport Studies: Faculty Publications

The menopausal transition is associated with increased risk for cardiovascular disease (CVD). Hot flashes (HF), a cardinal symptom of menopause, have been associated with increased CVD risk, particularly in perimenopausal women. Flow-mediated dilation (FMD) is an indicator of endothelial function and a subclinical CVD risk factor. Lower FMD has been associated with more HF. As moderate to vigorous physical activity (MVPA) is recognized to reduce CVD risk, our goal was to determine whether higher levels of MVPA change the relationship between HF and FMD in perimenopausal women. Healthy perimenopausal women had HF measured objectively using sternal skin conductance for 24 …

Sustainable Poultry Education For Undergraduate Students, A. C. Fanatico, A. Kollanoor Johny, A. Upadhyay, S. B. Bramall

Poultry Science Faculty Publications and Presentations

Sustainable poultry production can help address many critical socio- and environmental issues, including food insecurity, climate crisis, and loss of biodiversity. The education of undergraduate students in poultry science helps prepare a future workforce in poultry production and to develop responsible citizens for global engagement. The University of Connecticut has a poultry project, funded by U.S. Department of Agriculture's Sustainable Agriculture Systems, that researches the sustainability of meat chickens, particularly holistic health and alternatives to antibiotics. The project's education component is led by the Sustainable Development Department at Appalachian State University in partnership with the Universities of Connecticut, Minnesota, …