Life Sciences Commons^™

Starting Vegetable Seeds Indoors: Iii. Germination, Wesley Crump, Taun Beddes, Michael Caron, Milena Oliveira

All Current Publications

This third fact sheet in this series of four guides you through the actual process of starting seeds indoors. The materials you choose may not precisely match those mentioned here. However, the variables and processes discussed are still applicable.

A Genome-Wide Association Study To Detect Candidate Genes Controlling Iron And Manganese Accumulation In Walnut (Juglans Regia L.), Özge Doğanay, Erdal Orman, Bülent Yağmur, Muhammed Bahatti̇n Tanyolaç, Duygu Ateş

Turkish Journal of Agriculture and Forestry

The walnut is one of the important low-glycemic sources of vegetable protein that benefit human health. Iron (Fe) and Manganese (Mn) are two of the walnut’s important nutritional components. This study aimed to identify single-nucleotide polymorphism (SNP) markers associated with the concentrations of Mn and Fe in walnut kernels and the genetic variation in these concentrations. The population was evaluated for two different years using 154 walnut genotypes and genotyping was performed with 16,473 SNP markers. A genome-wide association study was performed on 154 genotypes. A total of five and 15 SNPs were identified in different walnut genotypes, which were …

Impact Of Particle Size On Physical And Functional Characteristics Of Baby Corn Powder, Ubaida Akbar, Prasad Rasane, Md Sabir Ahmed Mondol, Jyoti Singh, Vikas Nanda, Hasan Kiliçgün, Sawinder Kaur

Turkish Journal of Agriculture and Forestry

This study’s objective is to explore the influence of particle size on the characteristics and functionalities of baby corn powder. In food product development and application, particle properties are crucial. Sieve analysis using a series of standardized sieves determined the particle size distribution, ranging from >500 µm to 53 µm. The particles exhibited stability, with an average size of 208.8 nm, a polydispersity index of 0.133, and a zeta potential of –20 mV. Bulk density (BD) and true density (TD) showed an inverse relationship with particle size, with BD ranging from 0.552 to 0.238 gcm–3 for particle sizes >500 to …

Mapping Of Qtls Associated With Frost Tolerance In Olive (Olea Europaea L.), Öznur Çeti̇n, Nurengi̇n Mete, Handan Ölmez, Hati̇ce Gülen

Turkish Journal of Agriculture and Forestry

The olive (Olea europaea L.) is widely distributed due to its high adaptability to different environmental conditions. However,in recent years, sudden extreme temperature decreases and frost damage have caused significant yield and quality losses in some regions.This situation has led to the development of varieties with high frost tolerance, suitable for regions where cold damage may occur.It takes many years to develop varieties using classical breeding methods in olives. Therefore, early selection should be made usingbiotechnological methods. Markers about any trait provide a great advantage, especially in plant species with a very long juvenile sterilityperiod, as they allow for a …

The Role Of Brassinosteroids In Plant Physiological And Molecular Responses To Counter Salt Stress And Ensure Food Security: A Review And Future Perspectives, Muhammad Umair Hassan, Huang Guoqin, Muhammad Nawaz, Adnan Noor Shah, Tahir Abbas Khan, Muhammad Inzamam Ul Haq, Mehmood Ali Noor, Zhou Ping, Liu Qin, Yasser S. Mostafa, Saad A. Alamri, Melekber Sülüşoğlu Durul, Mehmet Ramazan Bozhüyük, Mohamed Hashem

Turkish Journal of Agriculture and Forestry

Excessive salinity poses serious threats to crop productivity and global food security. Salt stress reduces crop growth and final productivity by inducing ionic, osmotic, and oxidative damage. Applying plant growth hormones is thought to be an efficient method of reducing the negative effects of salinity. Brassinosteroids (BRs) are plant hormones that have demonstrated appreciable effects against different abiotic stresses. BRs can mitigate the deleterious impacts of salt stress by improving water and nutrient uptake, membrane stability, antioxidant activities, and osmolyte synthesis while maintaining the hormonal balance. Furthermore, BRs can also improve the metabolic and molecular responses of plants to help …

Chemical Composition And Larvicidal Toxicity Of Ricinus Communis L. (Euphorbiaceae) Extracts Against Larvae Of Thaumetopoea Pityocampa (Denis & Schiffermüller, 1775) (Lepidoptera: Notodontidae), Memi̇ş Kesdek, Şaban Kordali, Selçuk Küçükaydin

Turkish Journal of Agriculture and Forestry

In this study, the toxicities of extracts obtained from the seeds and leaves of castor bean (Ricinus communis L.) (Euphorbiaceae) plant against five larval stages of the pine processionary moth (PPM) (Thaumetopoea pityocampa (Denis & Schiffermüller, 1775) (Lepidoptera: Notodontidae)) were investigated under laboratory conditions. Petroleum ether, acetone, and methanol extracts were prepared at various concentrations (0.25, 0.5, and 1 mg/mL) and sprayed on each larval stage. Each test was performed with three replications and two repetitions. Sterile water + ethanol was used as a negative control and the commercial chemical Kormilin 25 WP (25% diflubenzuron) was used as a positive …

Identification And Functional Characterization Of The Icse1 Gene From Ilex Cornuta, Xiaoxi Zhang, Li Zhu, Weiwei Zhang, Feng Xu, Jiabao Ye, Yongling Liao

Turkish Journal of Agriculture and Forestry

Ilex cornuta (I. cornuta) is a traditional Chinese medicine that is rich in various secondary metabolites with pharmacological activities, among which triterpenoid saponins are one of the main active components. Squalene epoxidase (SE) is a key regulatory enzyme in the biosynthesis of triterpenoids. In this study, we isolated a squalene epoxidase (IcSE1) from I. cornuta and analyzed its molecular characteristics and functions. The results indicated that the total length of the IcSE1 sequence was 1942 bp, which includes a 1638 bp open reading frame (ORF) that encodes 545 amino acids. The theoretical isoelectric point (pI) and molecular weight of the …

Restore The Shore: A Guide To Lakeshore Revegetation In The Great Basin, Jes V. Braun, Rae Robinson, Annie L. Henry, Karin M. Kettenring

All Current Publications

Lakeshore vegetation supports many valuable aspects of lake ecosystems: foraging areas for birds, rearing grounds for fish, nutrient cycling, carbon storage, and aesthetic, cultural, and spiritual values. Yet, the vegetation of many lakeshores has been lost or heavily impacted by humans. What can be done to restore lakeshore vegetation to ensure that lakes remain healthy for future generations? In this guide, we summarize best practices for lakeshore revegetation (i.e., the process of actively reintroducing vegetation, usually native species, to a restoration site through seeding and planting) in the Great Basin.

Trpv4 Dominates High Shear-Induced Initial Traction Response And Long-Term Relaxation Over Piezo1, Mohanish Chandurkar, Manli Yang, Majid Rostami, Sangyoon J. Han

Michigan Tech Publications

Modulation of endothelial traction is critical for the responses of endothelial cells to fluid shear stress (FSS), which has profound implications for vascular health and atherosclerosis. Previously, we demonstrated that under high FSS, endothelial cells rapidly increase traction forces, followed by relaxation, with traction aligning in the flow direction. In contrast, low shear preconditioning induces a modest short-term increase in traction (min), followed by a secondary long-term (>14 hr) rise, with traction/cells aligning perpendicular to the flow. The upstream mechanosensors driving these responses, however, remain unknown. Here, we sought the roles of Piezo1 and TRPV4 ion channels in shear-induced …

Exploring Genetic Diversity: The Inheritance Of Protein And Mineral Contents In Dwarf Common Beans, Ercan Ceyhan, Ayşegül Korkmaz, Amjad Ali̇, Serdar Karadaş, Mustafa Harmankaya, Duran Şi̇mşek, Tolga Karaköy

Turkish Journal of Agriculture and Forestry

Beans are a vital plant-based protein, containing essential minerals that are necessary for human health. Their protein and mineral compositions vary due to factors including cultivar or variety, growth conditions, and processing methods. Global deficiencies in protein and minerals necessitate fortification, supplementation, and dietary diversification. The present study focuses on common bean (Phaseolus vulgaris) cultivars, aiming to understand their genetic variation and inheritance of protein and mineral contents. Five dwarf common bean cultivars (Lida, Beyzade, Ribera, Albeni, and Garrafol) were used in a full-diallel hybridization method and the experiment was conducted in Konya, Türkiye. The diallel analysis assessed both general …

Molecular Screening Of Septoria-Resistant Genes In Historical Turkish Bread Wheat Germplasm Using The Validated Gene Specific Ssr Markers, Amjad Ali, Fati̇h Ölmez, Muhammed Tatar, Parnaz Mortazavi, Muhammad Tanveer Altaf, Emi̇ne Burcu Turgay, Hüsnü Aktaş, Muhammad Azhar Nadeem, Jazib Javed, Jin-Ying Gou, Muhammad Aasim, Abdelfattah A. Dababat, Faheem Shehzad Baloch

Turkish Journal of Agriculture and Forestry

Septoria tritici blotch (STB), caused by Zymoseptoria tritici, poses a significant threat to global wheat production, particularly in Türkiye. Resistance breeding is the most sustainable and effective disease control method. Molecular markers, especially simple sequence repeat (SSR) markers are extensively employed in wheat breeding to enhance the efficacy. The primary objective of this study was to identify Stb resistance genes among 143 historical registered Turkish bread wheat genotypes released as commercial cultivars between 1963 to 2014, using 16 closely linked SSR markers. The findings revealed substantial genetic variation among the screened cultivars, with the Stb3 gene being the most prevalent, …

Enhancing Salt Tolerance And Yield Potential In Cotton: Insights From Physiological Responses, Genetic Variability, And Heterosis, Muhammad Mubashar Zafar, Abdul Razzaq, Zunaira Anwar, Aqsa Ijaz, Muhammad Zahid, Muhammad Shahid Iqbal, Ghulam Farid, Mahmoud F. Seleiman, Rana Qammar Uz Zaman, Abdur Rauf, Xuefei Jiang

Turkish Journal of Agriculture and Forestry

Soil salinity is a growing global concern, necessitating the development of salt-tolerant cotton cultivars to enhance the utilization of salt-affected soils and boost crop productivity. Salt tolerance is regulated by a complex interplay of genetic and physiological factors involving multiple genes. To investigate the genetic basis of salt stress tolerance, eight parental genotypes (four lines and four testers) and their 16 hybrid combinations were evaluated under normal and salt stress conditions (15 dS m⁻¹). Data were collected on various traits including the plant height (PH), number of bolls per plant (NBP), boll weight (BW), lint percentage (LP), seed cotton yield …

Nutritional And Microbiological Properties Of Cheese With Dried Elderberry And Red Grape Pomace, Andreea Dodan, Romina Alina Marc, Crina Carmen Mureşan, Carmen Rodica Pop, Delia Michiu, Miuța Rafila Filip, Roxana Nicoleta Rațu, Florina Stoica, Ioana Cristina Crivei, Alina Narcisa Postolache, Melinda Kovacs, Rodica Maria Sima, Mircea Valentin Muntean

Turkish Journal of Agriculture and Forestry

This study aimed to explore the use of grape byproducts such as red grape pomace and elderberry, in the form of bioactive powders to obtain three-month old cheese assortments. Grape pomace powder (GPP) and elderberry fruit powder (EFP) contain antioxidant components such as phenols, anthocyanins and flavonoids, and compounds characterized by high bioactivity. This study investigated not only the potential of incorporating different percentages (1%, 2%, and 3%) of GPP and EFP in cheese, to increase its nutritional value, but also the changes that occur during the 2 months of ripening. The results indicated that the incorporation of GPP and …

Transcriptome And Metabolome Analysis Of Low-Temperature Storage On Fruit Quality Deterioration Of Mangoes, Hanqing Cong, Ting Huo, Xinran Huang, Yeyuan Chen, Yibo Bai, Haiyan Luo, Xiaona Fu, Rongxiang Wang, Mahmoud F. Seleiman, Tanweer Ahmad, Pingyin Guan, Mohsin Bashir, Muhammad Asif, Fei Qiao

Turkish Journal of Agriculture and Forestry

Mango, a typical respiratory climacteric fruit, is susceptible to a short shelf life and quality deterioration during postharvest ripening. Although low temperature can prolong the storage time significantly, its effects on fruit quality have yet to be elucidated. Through a combination of transcriptome and metabolome analyses, this study aimed to elucidate the effects of low-temperature storage on the biosynthesis of volatile organic compounds (VOCs), flavonoids, sucrose, and the degradation of organic acids in mango. Carbohydrate-related metabolism maintained high activity when stored at 13 °C by upregulating the expression of certain gene members in the neutral invertase (NI), sucrose phosphate synthase …

Understanding The Role Of Thermal Stress In Modulating Growth, Yield, And Quality Attributes Of Tomato At Two Locations, Muhammad Uzair, Rabia Kalsoom, Kotb A. Attia, Irfan Ali, Tanveer Hussain, Asmaa M. Abushady, Haider Ali, Sajid Fiaz, Saima Jameel, Muhammad Usman Hameed, Rashid Iqbal, Muhammad Ramzan Khan

Turkish Journal of Agriculture and Forestry

Tomato is a well-recognized and extensively cultivated crop on a global scale. However, the development of tomato varieties is hindered by adverse environments, limited genetic diversity, and low rates of seed setting. In this study, we evaluated 21 genotypes at two different locations in Pakistan. We analyzed morphological traits including vine length, days to 50% flowering, days to fruit setting initiation, number of clusters per plant (NCP), and number of flowers per cluster (NFC); yield-related traits such as fruit setting percentage per cluster, fruit setting percentage per plant, number of marketable fruits per plant (NMFP), marketable fruit yield per plant …

Phytochemical Analysis And In Vitro And In Vivo Pharmacological Activities Of Cordia Myxa Extracts, Tatheer Fatima, Mirza Imran Shahzad, Adnan Noor Shah, Zhang Yi, Bushra Gillani, Iqra Hussain, Adina Gillani, Zeli̇ha Çi̇ftçi̇, Marcello Locatelli, Ashraf Atef Hatamleh, Munirah Abdullah Al-Dosary

Turkish Journal of Agriculture and Forestry

Cordia myxa is found in tropical regions of Pakistan, where it is commonly known as lasura. This study aimed to assess the preliminary results of phytochemical analysis and the pharmacological activities of C. myxa. After pulverizing the fresh plant, extracts were prepared using different solvents, including aqueous, methanol, ethanol, dichloromethane, ethyl acetate, and n-hexane extracts. These extracts were examined for total phenolic contents using standard biochemical tests and high performance liquid chromatography with a photodiode array. In further evaluations, antioxidant, antibacterial, antiviral, hen’s egg test on chorioallantoic membrane (HETCAM) assay, antipyretic, antiinflammatory, and antidiabetic activities were monitored. Using ferric reducing …

Bioactive Content And Antioxidant Capacity Of Some Plants And Fruits Grown In Türkiye, Gülay Özkan, Fatma Betül Sakarya, Bayram Yurt, Elwira Sieniawska, Yakup Yapar, Esra Çapanoğlu Güven

Turkish Journal of Agriculture and Forestry

Recently, some edible plants and fruits have undergone extensive research to identify potential health effects arising from their valuable nutritional and bioactive properties. The present study aims to investigate the phenolic compounds of nine edible plants and fruits throughout the gastrointestinal digestion model by comparing their total content and antioxidant activity. Of the species studied, Eremurus spectabilis and Gundelia tournefortii had the lowest total phenolic content, whereas Prunus armeniaca showed the highest value of 1026 ± 164 mg GAE/100 g. Except for Prunus armeniaca , the total phenolic contents of all samples were increased following gastric digestion, while the total …

Agronomic Performance Of Fenugreek Genotypes Grown Under Various Sowing And Nitrogen Rates In A Highland Environment, Furkan Çoban, Hakan Özer

Turkish Journal of Agriculture and Forestry

Fenugreek is an important medicinal plant used in food, medicine, and other industries. In recent years, there has been increased interest in the fenugreek plant, with increased support given for fenugreek in Türkiye. However, there is a need for sound recommendations on agronomic practices such as variety selection, plant density, and fertilization due to local differences in the climate and soil structure. This research was conducted to determine the yield and some agricultural characteristics of two fenugreek genotypes (Turkish and Iranian) grown under different nitrogen (N) (0, 20, 40, 60, and 80 kg ha–1) and sowing (20, 40, and 60 …

Author Correction: Orthogonal And Multiplexable Genetic Perturbations With An Engineered Prime Editor And A Diverse Rna Array, Qichen Yuan, Hongzhi Zeng, Tyler C Daniel, Qingzhuo Liu, Yongjie Yang, Emmanuel C Osikpa, Qiaochu Yang, Advaith Peddi, Liliana M Abramson, Boyang Zhang, Yong Xu, Xue Gao

Faculty, Staff and Students Publications

Correction to: Nature Communications 10.1038/s41467-024-55134-9, published online 30 December 2024

In the version of the article initially published, there were errors in Fig. 3d, where in the x-axis labels now reading “GPP-shRNA1, GPP-shRNA2, GPP-shRNA3…,” the second and third labels were duplicates of the first. The figure is amended in the HTML and PDF versions of the article.

Complete Genome Sequence Of A Penicillin-Resistant Fusobacterium Necrophorum Subsp Funduliforme Isolate From A Tonsillitis Patient, Bibek G C, Anders Jensen, Chenggang Wu

Faculty, Staff and Student Publications

We report the complete genome sequence of a penicillin-resistant Fusobacterium necrophorum subsp. funduliforme isolate, AJ79, from a tonsillitis patient. The AJ79 genome consists of a chromosome (2,440,359 bp) and plasmid (9,887 bp), providing insights into the genetic basis of penicillin resistance in F. necrophorum and its implications for treating tonsillitis.

Bbox1 Restrains Tbk1-Mtorc1 Oncogenic Signaling In Clear Cell Renal Cell Carcinoma, Chengheng Liao, Lianxin Hu, Liwei Jia, Jin Zhou, Tao Wang, Kangsan Kim, Hua Zhong, Hongwei Yao, Lei Dong, Lei Guo, Qian Liang, Cheng Zhang, Fangzhou Zhao, Jun Fang, Hongyi Liu, Shina Li, Lin Xu, Jeremy M Simon, Srinivas Malladi, Payal Kapur, James Brugarolas, Ralph J Deberardinis, Qing Zhang

Faculty, Staff and Student Publications

Clear cell renal cell carcinoma (ccRCC), a metabolic disease originating from renal proximal convoluted tubule (PCT) epithelial cells, remains incompletely understood in terms of its initiating signaling events. Here, we identify γ-butyrobetaine hydroxylase 1 (BBOX1), a key enzyme in carnitine synthesis predominantly expressed in PCT cells, as a tumor suppressor in ccRCC. BBOX1 expression is lost during ccRCC malignant transformation, and its restoration reduces cell viability in physiological medium and inhibits xenograft tumor growth. Transcriptomic analyses reveal that BBOX1 suppresses critical metabolic pathways including mTORC1 signaling and glycolysis in ccRCC. Further, we identify TANK-binding kinase 1 (TBK1) as an essential …

Engineered Immunomodulatory Extracellular Vesicles From Epithelial Cells With The Capacity For Stimulation Of Innate And Adaptive Immunity In Cancer And Autoimmunity, Xin Luo, Fernanda G Kugeratski, Dara P Dowlatshahi, Hikaru Sugimoto, Kent A Arian, Yibo Fan, Li Huang, Danielle Wills, Sergio Lilla, Kelly Hodge, Sara R Zanivan, Valerie S Lebleu, Kathleen M Mcandrews, Raghu Kalluri

Faculty, Staff and Student Publications

Extracellular vesicles (EVs) are generated by all cells. Systemic administration of allogenic EVs derived from epithelial and mesenchymal cells have been shown to be safe, despite carrying an array of functional molecules, including thousands of proteins. To address whether epithelial cells derived EVs can be modified to acquire the capacity to induce immune response, we engineered 293T EVs to harbor the immunomodulatory molecules CD80, OX40L and PD-L1. We demonstrated abundant levels of these proteins on the engineered cells and EVs. Functionally, the engineered EVs efficiently elicited positive and negative co-stimulation of human and murine T cells. In the setting of …

Nprl2 Gene Therapy Induces Effective Antitumor Immunity In Kras/Stk11 Mutant Anti-Pd1 Resistant Metastatic Non-Small Cell Lung Cancer (Nsclc) In A Humanized Mouse Model, Ismail M Meraz, Mourad Majidi, Renduo Song, Feng Meng, Lihui Gao, Qi Wang, Jing Wang, Elizabeth J Shpall, Jack A Roth

Faculty, Staff and Student Publications

Expression of NPRL2/TUSC4, a tumor-suppressor gene, is reduced in many cancers including NSCLC. Restoration of NPRL2 induces DNA damage, apoptosis, and cell-cycle arrest. We investigated NPRL2 antitumor immune responses in aPD1R/KRAS/STK11^mt NSCLC in humanized-mice. Humanized-mice were generated by transplanting fresh human cord blood-derived CD34 stem cells into sub-lethally irradiated NSG mice. Lung-metastases were developed from KRAS/STK11^mt/aPD1R A549 cells and treated with NPRL2 w/wo pembrolizumab. NPRL2-treatment reduced lung metastases significantly, whereas pembrolizumab was ineffective. Antitumor effect was greater in humanized than non-humanized-mice. NPRL2 + pembrolizumab was not synergistic in KRAS/STK11^mt/aPD1R tumors but was …

Regression Modeling Of Cumulative Incidence Function For Left-Truncated Right-Censored Competing Risks Data: A Modified Pseudo-Observation Approach, Rong Rong, Jing Ning, Hong Zhu

Faculty, Staff and Student Publications

Statistical methods have been developed for regression modeling of the cumulative incidence function (CIF) given left-truncated right-censored competing risks data. Nevertheless, existing methods typically involve complicated weighted estimating equations or nonparametric conditional likelihood function and often require a restrictive assumption that censoring and/or truncation times are independent of failure time. The pseudo-observation (PO) approach has been used in regression modeling of CIF for right-censored competing risks data under covariate-independent censoring or covariate-dependent censoring. We extend this approach to left-truncated right-censored competing risks data and propose to directly model the CIF based on POs, under general truncation and censoring mechanisms. We …

Repeat Expansion In A Fragile X Model Is Independent Of Double Strand Break Repair Mediated By Pol Θ, Rad52, Rad54 Or Rad54b, Bruce E Hayward, Geum-Yi Kim, Carson J Miller, Cai Mccann, Megan G Lowery, Richard D Wood, Karen Usdin

Faculty, Staff and Student Publications

Microsatellite instability is responsible for the human repeat expansion diseases (REDs). The mutagenic process differs from classical cancer-associated microsatellite instability (MSI) in that it requires the mismatch repair proteins that normally protect against MSI. LIG4, an enzyme essential for non-homologous end-joining (NHEJ), the major pathway for double-strand break repair (DSBR) in mammalian cells, protects against expansion in mouse models. Thus, NHEJ may compete with the expansion pathway for access to a common intermediate. This raises the possibility that expansion involves an NHEJ-independent form of DSBR. Pol θ, a polymerase involved in the theta-mediated end joining (TMEJ) DSBR pathway, has been …

Starting Vegetable Seeds Indoors: Iv. Seedling Culture And Transplanting, Wesley Crump, Taun Beddes, Michael Caron, Milena Oliveira

All Current Publications

The period between seedling emergence and planting outdoors for the growing season is a time of rapid plant growth and development and is critical to long-term production success. In this last fact sheet in this series, we cover the main topics crucial to growing seedlings into quality, usable plants for outdoor production, including air circulation, temperature, light, water, fertilizer, indoor transplanting, hardening off, and transplanting outside.

Exploring The Therapeutic Potential Of Resveratrol In Substance Use Disorders: A Systematic Review Of Its Effects On Kcc2 Expression, Lauren R. Nelson, Ashlyn Hughes, Jory Winward, Natalie Phanco, Andrew Payne Phd

Annual Research Symposium

No abstract provided.

Investigating The Effects Of Resveratrol On Opioid-Induced Hyperalgesia, Bryana Cox, Thien Do, Ashlyn Hughes, Zane Kingsbury, Keara Leroy, Andrew J. Payne

Annual Research Symposium

Opioid-induced hyperalgesia (OIH) is an adverse effect of chronic opioid therapy, characterized by increased sensitivity to pain. This study will seek to determine if using resveratrol supplementation as an indirect enhancer to increase the expression of the KCC2 membrane transporter can alleviate the symptoms of OIH in human subjects.

Use Of Synthetic Water-Soluble Kcc2 To Modulate Chloride Homeostasis In Cultured Dopamine-Neuron-Like Cells, Mason Terry, Andrew Payne, Dario Mizrachi

Annual Research Symposium

No abstract provided.

A Morphological Investigation Of Alcohol-Induced Persister Formation, Isaac Young, Victor Jimenez

Annual Research Symposium

No abstract provided.